A method for rapid detection of Po nuclides in rat lung tissue 210 A method for rapid detection of Po nuclides in rat lung tissue

Abstract

The application relates to a method for detecting Po in rat lung tissue 210 The method for rapidly detecting Po nucleuses comprises the following steps: preparing a self-deposition liquid, self-depositing alpha nucleuses in the self-deposition liquid on a silver sheet, measuring alpha nucleus counts recovered through the self-deposition method, obtaining the alpha nucleus counts recovered through the self-deposition method, respectively calculating the alpha nucleus counts recovered through the self-deposition method, calculating the Po nucleuses in the self-deposition liquid, calculating the alpha nucleus counts in the self-deposition liquid, calculating the recovery rate, calculating the activity of the Po nucleuses in the self-deposition liquid, and calculating the activity of the Po nucleuses in the rat lung tissue. 209 The method for rapidly detecting Po nucleuses comprises the following steps: preparing a self-deposition liquid, self-depositing alpha nucleuses in the self-deposition liquid on a silver sheet, measuring alpha nucleus counts recovered through the self-deposition method, obtaining the alpha nucleus counts recovered through the self-deposition method, respectively calculating the alpha nucleus counts recovered through the self-deposition method, calculating the Po nucleuses in the self-deposition liquid, calculating the alpha nucleus counts in the self-deposition liquid, calculating the recovery rate, calculating the activity of the Po nucleuses in the self-deposition liquid, and calculating the activity of the Po nucleuses in the rat lung tissue. 210 The method for rapidly detecting Po nucleuses comprises the following steps: preparing a self-deposition liquid, self-depositing alpha nucleuses in the self-deposition liquid on a silver sheet, measuring alpha nucleus counts recovered through the self-deposition method, obtaining the alpha nucleus counts recovered through the self-deposition method, respectively calculating the alpha nucleus counts recovered through the self-deposition method, calculating the Po nucleuses in the self-deposition liquid, calculating the alpha nucleus counts in the self-deposition liquid, calculating the recovery rate, calculating the activity of the Po nucleuses in the self-deposition liquid, and calculating the activity of the Po nucleuses in the rat lung tissue. 210 The method for rapidly detecting Po nucleuses comprises the following steps: preparing a self-deposition liquid, self-depositing alpha nucleuses in the self-deposition liquid on a silver sheet, measuring alpha nucleus counts recovered through the self-deposition method, obtaining the alpha nucleus counts recovered through the self-deposition method, respectively calculating the alpha nucleus counts recovered through the self-deposition method, calculating the Po nucleuses in the self-deposition liquid, calculating the alpha nucleus counts in the self-deposition liquid, calculating the recovery rate, calculating the activity of the Po nucleuses in the self-deposition liquid, and calculating the activity of the Po nucleuses in the rat lung tissue. 210 The method for rapidly detecting Po nucleuses comprises the following steps:

Description

Technical Field

[0001] The present invention belongs to the technical field of radioactive material analysis and measurement, and specifically relates to a method for 210 Rapid detection method for Po nuclide. Background Art

[0002] Smoking is one of the high-risk factors for lung cancer.

[0003] Epidemiological studies have confirmed that smoking can cause a variety of diseases, including lung cancer, chronic bronchitis, emphysema, arteriosclerosis, diabetes, heart disease, kidney disease, and cerebrovascular accidents. For a long time, research on the etiology of lung cancer has focused solely on the carcinogenicity of chemical factors, such as benzopyrene and nicotine, while rarely examining radioactive carcinogens, particularly the relationship between radionuclides in cigarettes and lung cancer.

[0004] Cigarettes are rich in radon progeny at a high level, including polonium ( 210 Po) is considered to be the main component of radionuclides in cigarettes. 210 Po will release alpha particles during the decay process and become stable 206 Pb, alpha particles lose energy quickly and have the weakest penetrating power among all ionizing radiations. They can be blocked by human skin or a piece of paper. Therefore, as long as they are not swallowed, inhaled or injected into the human body, they have little impact. It is precisely because of the high energy, short range, strong radiobiological effects and cytotoxicity of alpha particles that they can be combined with tumor-targeted carriers to construct tumor-targeted radiotherapy drugs. They have good application prospects in the internal radiotherapy of scattered cancers and micrometastatic cancers, such as 211 At 213 Bi, 212 Bi, 225 Ac, 223 The radionuclides such as Ra have entered clinical trials. 223 Ra and 213 Bi.

[0005] However, smokers inhale smoke into their lungs during smoking. 210 Po slowly accumulates in the lung tissue and continuously releases alpha rays, continuously causing internal irradiation to the lung tissue. Alpha rays can affect the human immune function. The direct and indirect effects of alpha particles will damage the DNA of normal cells and even cause cancer. This is one of the important mechanisms by which smoking causes cancer. According to foreign research reports, smoking 30 cigarettes a day is equivalent to taking a chest X-ray once; for those who smoke a pack and a half of cigarettes a day, the amount of radiation absorbed by the lungs in a year is equivalent to receiving 300 chest X-rays; the amount of radiation inhaled by a cigarette is equivalent to receiving 300 chest X-rays. 210 Po, the high-intensity α-rays it produces are almost equivalent to the total amount of natural radiation a person receives within 24 hours.210 Study on Po internal radiation dose. Summary of the Invention

[0006] In view of the defects in the prior art, the present invention aims to provide a method for 210 The rapid detection method for Po nuclides uses the method to achieve an α nuclide recovery rate of over 70% for the lung tissues of smoking rats of different genders, which can provide a cause analysis for smoking-induced lung cancer and provide a detection basis for the metabolism of α nuclides in the body and the development of nuclear medicine in the future.

[0007] In order to achieve the above purpose, the technical solution adopted by the present invention is: a rat lung tissue 210 A method for rapid detection of Po nuclides, comprising the following steps:

[0008] S1. Preparation of autodeposition solution: using concentrated nitric acid, hydrogen peroxide, perchloric acid, concentrated hydrochloric acid to digest the added 209 Po standard solution was used to infect rat lung tissue and 209 After digestion of rat lung tissues exposed to the Po standard solution, hydrochloric acid, hydroxylamine hydrochloride and ascorbic acid were added to prepare the autoprecipitation solution;

[0009] S2, autodeposition: the α nuclides in the autodeposition liquid are autodeposited on the silver sheet;

[0010] S3, wash and dry the silver sheet, measure the α nuclide count recovered by self-deposition, and obtain the recovered α nuclide counts by self-deposition. 209 Po nuclide counting, recovery by autodeposition 210 Po nuclide counting;

[0011] S4, calculate recovery rate: using the self-deposition recovery 209 Po nuclide count divided by 209 Count the α nuclides in the Po standard solution and obtain the recovery rate;

[0012] S5. Calculate the lung tissue of the infected rats 210 Activity of Po nuclide: Using the self-deposition recovery 210 The Po nuclide count was divided by the recovery rate to obtain the number of nuclide counts in the lung tissue of the infected rats. 210 Activity of Po nuclide.

[0013] Further, the concentrated nitric acid, hydrogen peroxide, perchloric acid and concentrated hydrochloric acid are used to digest the added 209 The specific steps of exposing rat lung tissue to the Po standard solution include the following:

[0014] S11, concentrated nitric acid digestion: freeze-dry the lung tissue of the infected rat and grind it into a powder sample. Accurately weigh a certain amount of the powder sample and put it into the first beaker. Add a specified amount of 209 Po standard solution, a set amount of concentrated nitric acid, and the first beaker was left overnight;

[0015] S12, hydrogen peroxide digestion: heating the first beaker at 130-150° C. for 25-35 minutes, cooling for a certain period of time, adding hydrogen peroxide solution to the first beaker, and heating the first beaker at 90-110° C. for 5-15 minutes;

[0016] S13, filtering: After the first beaker is cooled, filtering is performed, and the filtered filtrate is retained in the second beaker;

[0017] S14, perchloric acid digestion: evaporate the second beaker at 100-120°C to dryness, add 0.5-1.5 mL of perchloric acid, and evaporate at 100-120°C until no white smoke is emitted;

[0018] S15. Digestion with concentrated hydrochloric acid: add 1-3 mL of concentrated hydrochloric acid to the second beaker containing the filtrate, and evaporate to dryness at 100-120°C.

[0019] Furthermore, after the filtering step in step S13, the following specific steps are also included:

[0020] S131, transferring the filter residue from the filtration step back into the first beaker, adding the set amount of concentrated nitric acid and letting it stand for 2.5 to 3.5 hours;

[0021] S132, second filtration: repeat step S12, and perform a second filtration after the sample is cooled, and retain the filtrate of the second filtration in the second beaker and combine it with the filtrate of the first filtration.

[0022] Furthermore, after the concentrated hydrochloric acid digestion step in step S15, the following specific steps are also included:

[0023] Repeat step S15: add 1-3 mL of concentrated hydrochloric acid to the second beaker and evaporate to dryness at 100-120°C.

[0024] Furthermore, the step of adding hydrochloric acid, hydroxylamine hydrochloride, and ascorbic acid to prepare the autodeposition solution in step S1 includes the following specific steps:

[0025] Add 0.5 mol·L -1 40-60 mL of hydrochloric acid, hydroxylamine hydrochloride solution, and ascorbic acid were added, and the second beaker was shaken to prepare the autodeposition solution.

[0026] Furthermore, in step S2, one side of the silver sheet is sprayed with paint, and the other side of the silver sheet is polished to serve as a self-deposition surface of the silver sheet, and then rinsed with deionized water and placed on a bracket provided in the second beaker;

[0027] During the self-deposition process, the silver sheet is placed on a support provided in the second beaker with the self-deposition surface facing upward.

[0028] Furthermore, in the autodeposition process of step S2, the autodeposition temperature is controlled at 95° C. and the autodeposition time is 2.5 h.

[0029] Furthermore, in step S3, the method of washing and drying the silver sheet is:

[0030] The removed silver sheet was rinsed with deionized water, then soaked in anhydrous ethanol for a set time and then rinsed with deionized water. The silver sheet was then placed in a culture dish with the self-deposition side facing downward, and the culture dish was placed in an oven to dry.

[0031] Furthermore, in step S3, the method for measuring the count of alpha nuclides recovered by self-deposition is:

[0032] The silver sheet was placed on an α spectrometer with the self-deposition side facing upwards and measured for 48 hours to obtain the α nuclide counts recovered by self-deposition and the α nuclide counts recovered by self-deposition were obtained respectively. 209 Po nuclide counting, recovery by autodeposition 210 Po nuclide counting.

[0033] The beneficial effects of the present invention are: using a rat lung tissue 210 The rapid detection method of Po nuclides can be achieved by preparing a self-deposition liquid, wherein the α nuclides in the self-deposition liquid are self-deposited on a silver sheet, and the counts of the α nuclides recovered by the self-deposition method are measured to obtain the recovered α nuclides by the self-deposition method. 209 Po nuclide counting, recovery by autodeposition 210 Po radionuclide count, recovery rate, and the number of radionuclides in the lung tissue of the infected rats 210 The activity of Po nuclide in the lung tissue of the infected rats was achieved through the following steps. 210 The recovery rate of Po nuclides is greater than 70%, which proves that the method provided by the present invention has good detection efficiency and high sensitivity. Moreover, the method provided by the present invention sets reasonable conditions for the preparation of the autodeposition liquid and the autodeposition conditions. It only needs to place the obtained autodeposition silver sheet on the α spectrometer for 48 hours to obtain the recovered Po nuclides through autodeposition. 209 Po nuclide counting, recovery by autodeposition 210Po nuclide counting significantly shortens the time required to measure alpha nuclides recovered through self-deposition using existing methods. This method quantitatively analyzes alpha nuclides in rat lung tissue after exposure to the poison, resulting in a rapid and effective analytical test method. This method can provide insights into the causes of smoking-induced lung cancer and provide a basis for future research into the in vivo metabolism of alpha nuclides and the development of nuclear medicines. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] Figure 1 The present invention is a rat lung tissue 210 Schematic diagram of the process for rapid detection of Po nuclides. DETAILED DESCRIPTION

[0035] The technical solutions in the embodiments of the present invention will be further clearly and completely described below in conjunction with the drawings and examples. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0036] like Figure 1 As shown, the embodiment of the present invention provides a rat lung tissue 210 A method for rapid detection of Po nuclides, comprising the following steps:

[0037] S1. Preparation of autodeposition solution: using concentrated nitric acid, hydrogen peroxide, perchloric acid, concentrated hydrochloric acid to digest the added 209 Po standard solution was used to infect rat lung tissue and 209 After digestion of rat lung tissues exposed to the Po standard solution, hydrochloric acid, hydroxylamine hydrochloride and ascorbic acid were added to prepare the autoprecipitation solution;

[0038] Optionally, the concentrated nitric acid, hydrogen peroxide, perchloric acid, and concentrated hydrochloric acid are used to digest the added 209 The specific steps of exposing rat lung tissue to the Po standard solution include the following:

[0039] S11, concentrated nitric acid digestion: freeze-dry the lung tissue of the infected rat and grind it into a powder sample. Accurately weigh a certain amount of the powder sample and put it into the first beaker. Add a specified amount of 209 Po standard solution, a set amount of concentrated nitric acid, and leave the first beaker overnight.

[0040] Specifically, about 1g of rat lung tissue infected with the poison was freeze-dried and ground into a powder sample. 0.5g of the powder sample was accurately weighed and placed in a first beaker. 209 Add 0.2 mL of Po standard solution and 10 mL of concentrated nitric acid, and leave the first beaker overnight.

[0041] The concentration of the concentrated HNO3 is greater than 8 mol / L; under the condition of concentrated HNO3, the protein in the rat lung tissue is destroyed and separated.

[0042] S12, hydrogen peroxide digestion: on the next day, heat the first beaker on a 140°C hot plate for 30 minutes, cool for 10 minutes, add 3 mL of hydrogen peroxide solution after the sample cools slightly, and heat on a 100°C hot plate for 10 minutes;

[0043] Specifically, the mass percentage of H2O2 in the hydrogen peroxide solution is 30%; H2O2 can decompose colored substances in rat lung tissue to clarify the sample.

[0044] S13, first filtration: After the sample in the first beaker has cooled for 30 minutes, perform the first filtration, and retain the filtrate of the first filtration in the second beaker;

[0045] Considering that there may be encapsulated 209 Po, therefore, after the first filtration in step S13, a second filtration is performed, and the filtrate of the second filtration is combined with the filtrate of the first filtration; the specific steps are as follows:

[0046] S131, transferring the filter residue from the first filtration back to the first beaker, adding the set amount of concentrated nitric acid and letting it stand for 3 hours;

[0047] S132, second filtration: place the first beaker on a 140°C hot plate and heat for 30 minutes, cool for 10 minutes, add 3 mL of hydrogen peroxide solution after the sample cools slightly, and place the beaker on a 100°C hot plate and heat for 10 minutes (repeat step S12);

[0048] After the sample has cooled for 30 minutes, it is filtered for the second time, and the filtrate from the second filtration is retained in the second beaker and combined with the filtrate from the first filtration.

[0049] S14. Perchloric acid digestion: Place the second beaker containing all the filtrate in a 110°C sand bath and evaporate until nearly dry. Add 1 mL of perchloric acid and evaporate in a 110°C sand bath until no white smoke is emitted.

[0050] Specifically, the mass percentage of the perchloric acid is 72%; the perchloric acid combined with a 110° C. sand bath can be used to decompose rat lung tissue and other reducing substances remaining in the filtrate of the sample, and then evaporate to remove the decomposition products.

[0051] S15, concentrated hydrochloric acid digestion: add 2 mL of concentrated hydrochloric acid to the second beaker containing the filtrate, and evaporate to near dryness in a sand bath at 110°C;

[0052] Then, step S15 was repeated to add 2 mL of concentrated hydrochloric acid to the second beaker, and evaporated to near dryness in a sand bath at 110° C.

[0053] Specifically, the mass percentage of the concentrated hydrochloric acid is 37%, which is used to further digest the residual rat lung tissue.

[0054] Optionally, the step of adding hydrochloric acid, hydroxylamine hydrochloride, and ascorbic acid to prepare the autodeposition solution in step S1 includes the following specific steps:

[0055] Add 50 mL of 0.5 mol·L -1 hydrochloric acid, 3 drops of hydroxylamine hydrochloride solution, 2 g of ascorbic acid, and gently shake for 3 minutes to prepare an autoprecipitation solution.

[0056] Specifically, the mass percentage of the hydroxylamine hydrochloride solution is 50%.

[0057] The hydroxylamine hydrochloride solution and ascorbic acid can be used to remove possible residual perchloric acid and other oxidizing substances that may remain in the sample; and adjust the valence of Po ions in the autodeposition solution to tetravalent.

[0058] S2, autodeposition: α nuclides in the autodeposition liquid are self-deposited on the silver sheet;

[0059] 210 The principle of Po nuclide self-deposition on the silver sheet: the reduction electrode potential is a measure of the ability of the electrode to be reduced. The larger the reduction electrode potential value, the stronger the ability of the electrode to be reduced. Since the standard reduction electrode potential of silver is 0.7996V and the standard reduction electrode potential of Po is 0.76V, but there are almost no silver ions in the self-deposition solution, the electrode potential of silver is lower than the electrode potential of Po, so that the Po cations in the self-deposition solution can self-deposit on the silver sheet; at the same time, since the standard reduction electrode potential of silver is high enough, higher than other impurity ions in the self-deposition solution, other impurity ions will not self-deposit on the silver sheet during the self-deposition process. Therefore, it can be considered that the α nuclide self-deposited on the silver sheet is 209 Po nuclide and 210 Po nuclide.

[0060] Specifically, one side of the silver sheet is sprayed with red paint, and the other side of the silver sheet is polished with sandpaper and rinsed with deionized water; the polished side of the silver sheet (i.e., the self-deposition side of the silver sheet) is placed upward on a bracket set in the second beaker, a stirring magnet is placed in the second beaker, and the second beaker is placed on a constant temperature magnetic stirrer for self-deposition.

[0061] The self-deposition temperature during the self-deposition process is controlled at 95° C., and the self-deposition time is 2.5 h.

[0062] The silver sheet is 0.2mm thick high-purity silver sheet (impurity content <10ppm).

[0063] S3, washing and drying the silver sheet, and measuring the alpha nuclide count recovered by autodeposition; comprising the following specific steps:

[0064] Wash and dry the silver sheet: After removing the silver sheet, rinse it with deionized water, then soak it in anhydrous ethanol for 30 minutes and rinse it with deionized water again. Then, place the silver sheet in a Petri dish with the silver sheet deposited surface facing down. Label the Petri dish and dry it in a 120°C oven for 2 hours.

[0065] Measure the α nuclide counts recovered by self-deposition: Place the cooled silver sheet (self-deposition side facing up) on the α spectrometer and measure for 48 hours to obtain the α nuclide counts recovered by self-deposition. 209 Po nuclide counting, recovery by autodeposition 210 Po nuclide counting.

[0066] The alpha spectrometer is an American ORTEC Alpha Suite single-channel to eight-channel alpha spectrometer.

[0067] S4, calculate recovery rate: using the self-deposition recovery 209 Po nuclide count divided by 209 The α nuclides in the Po standard solution are counted and the recovery rate is obtained, that is, 210 Po nuclide recovery rate;

[0068] S5. Calculate the lung tissue of the infected rats 210 Activity of Po nuclide: Using the self-deposition recovery 210 The Po nuclide count divided by the recovery rate can be obtained to obtain the number of Po nuclides in the lung tissue of the infected rats. 210 Activity of Po nuclide.

[0069] Verification Example

[0070] To verify the reliability and accuracy of the method provided in this embodiment, lung tissue from two groups of rats exposed to passive smoking for one year was collected. Each group of rats included six female rats (numbered F101-F106 and F201-F206) and six male rats (numbered M107-F112 and M207-F212). Both groups of rats were exposed to passive smoking for one year.

[0071] Each number of rat lung tissue exposed to the poison was tested by the method provided in this embodiment, and the recovery rate, the concentration of the rat lung tissue exposed to the poison were obtained. 210 The activity of Po nuclides is shown in Table 1 below.

[0072] Table 1 Recovery rate and lung tissue of infected rats 210 Activity of Po nuclide

[0073]

[0074]

[0075] As can be seen from Table 1, the method provided in this embodiment is to prepare an autodeposition liquid, wherein the α nuclides in the autodeposition liquid are self-deposited on the silver sheet, and the α nuclides recovered by the autodeposition method are measured to obtain the recovered α nuclides by the autodeposition method. 209 Po nuclide counting, recovery by autodeposition 210 Po radionuclide count, recovery rate, and the number of radionuclides in the lung tissue of the infected rats 210 The activity of Po nuclide was obtained from rat lung tissue 210 The recovery rates of Po nuclides are all greater than 70%, which proves that the method provided by this embodiment has good detection efficiency and high sensitivity.

[0076] In addition, the method provided in this embodiment provides reasonable conditions for the preparation of the self-deposition liquid and the self-deposition conditions. It is only necessary to place the obtained self-deposition silver sheet on the α spectrometer for measurement for 48 hours to obtain the self-deposition recovered silver. 209 Po nuclide counting, recovery by autodeposition 210 The time required to count Po nuclides is significantly shorter than that of existing methods for measuring the counts of α nuclides recovered by self-deposition.

[0077] The methods described herein are not limited to the specific embodiments described. The above embodiments are merely illustrative of the present invention, and the present invention may also be implemented in other specific ways or in other specific forms without departing from the gist or essential characteristics of the present invention. Therefore, the embodiments described herein are to be considered in all respects as illustrative and not restrictive. The scope of the present invention is to be determined by the appended claims, and any variations that are equivalent to the intent and scope of the claims are intended to be within the scope of the present invention.

Claims

1. A rat lung tissue 210 The method for rapid detection of Po nuclides is characterized by: The method comprises the following steps: S1. Preparation of autodeposition solution: using concentrated nitric acid, hydrogen peroxide, perchloric acid, concentrated hydrochloric acid to digest the added 209 Po standard solution was used to infect rat lung tissue and 209 After digestion of rat lung tissues exposed to the Po standard solution, hydrochloric acid, hydroxylamine hydrochloride and ascorbic acid were added to prepare the autoprecipitation solution; S2, autodeposition: the α nuclides in the autodeposition liquid are autodeposited on the silver sheet; S3, wash and dry the silver sheet, measure the α nuclide count recovered by self-deposition, and obtain the recovered α nuclide counts by self-deposition. 209 Po nuclide counting, recovery by autodeposition 210 Po nuclide counting; S4, calculate recovery rate: using the self-deposition recovery 209 Po nuclide count divided by 209 Count the α nuclides in the Po standard solution and obtain the recovery rate; S5. Calculate the lung tissue of the infected rats 210 Activity of Po nuclide: Using the self-deposition recovery 210 The Po nuclide count was divided by the recovery rate to obtain the number of nuclide counts in the lung tissue of the infected rats. 210 Activity of Po nuclide.

2. A rat lung tissue according to claim 1 210 The method for rapid detection of Po nuclides is characterized by: In step S1, concentrated nitric acid, hydrogen peroxide, perchloric acid and concentrated hydrochloric acid are used to digest the 209 The specific steps of exposing rat lung tissue to the Po standard solution include the following: S11, concentrated nitric acid digestion: freeze-dry the lung tissue of the infected rat and grind it into a powder sample. Accurately weigh a certain amount of the powder sample and put it into the first beaker. Add a specified amount of 209 Po standard solution, a set amount of concentrated nitric acid, and the first beaker was left overnight; S12, hydrogen peroxide digestion: heating the first beaker at 130-150° C. for 25-35 minutes, cooling for a certain period of time, adding hydrogen peroxide solution to the first beaker, and heating the first beaker at 90-110° C. for 5-15 minutes; S13, filtering: After the first beaker is cooled, filtering is performed, and the filtered filtrate is retained in the second beaker; S14, perchloric acid digestion: evaporate the second beaker at 100-120°C to dryness, add 0.5-1.5 mL of perchloric acid, and evaporate at 100-120°C until no white smoke is emitted; S15. Digestion with concentrated hydrochloric acid: add 1-3 mL of concentrated hydrochloric acid to the second beaker containing the filtrate, and evaporate to dryness at 100-120°C.

3. A rat lung tissue according to claim 2 210 The method for rapid detection of Po nuclides is characterized by: After the filtering step in step S13, the following specific steps are also included: S131, transferring the filter residue from the filtration step back into the first beaker, adding the set amount of concentrated nitric acid and letting it stand for 2.5 to 3.5 hours; S132, second filtration: repeat step S12, and perform a second filtration after the sample is cooled, and retain the filtrate of the second filtration in the second beaker and combine it with the filtrate of the first filtration.

4. A rat lung tissue according to claim 3 210 The method for rapid detection of Po nuclides is characterized by: After the concentrated hydrochloric acid digestion step in step S15, the following specific steps are also included: Repeat step S15: add 1-3 mL of concentrated hydrochloric acid to the second beaker and evaporate to dryness at 100-120°C.

5. A rat lung tissue according to claim 2 210 The method for rapid detection of Po nuclides is characterized by: The step S1 of adding hydrochloric acid, hydroxylamine hydrochloride, and ascorbic acid to prepare the autodeposition solution comprises the following specific steps: Add 0.5 mol·L -1 40-60 mL of hydrochloric acid, hydroxylamine hydrochloride solution, and ascorbic acid were added, and the second beaker was shaken to prepare the autodeposition solution.

6. A rat lung tissue according to claim 2 210 The method for rapid detection of Po nuclides is characterized by: In step S2, one side of the silver sheet is sprayed with paint, and the other side of the silver sheet is polished to serve as a self-deposition surface of the silver sheet, and then rinsed with deionized water and placed on a bracket provided in the second beaker; During the self-deposition process, the silver sheet is placed on a support provided in the second beaker with the self-deposition surface facing upward.

7. A rat lung tissue according to claim 6 210 The method for rapid detection of Po nuclides is characterized by: In the autodeposition process of step S2, the autodeposition temperature is controlled at 95° C. and the autodeposition time is 2.5 h.

8. A rat lung tissue according to claim 6 210 The method for rapid detection of Po nuclides is characterized by: In step S3, the method of washing and drying the silver sheet is: The removed silver sheet was rinsed with deionized water, then soaked in anhydrous ethanol for a set time and then rinsed with deionized water. The silver sheet was then placed in a culture dish with the self-deposition side facing downward, and the culture dish was placed in an oven to dry.

9. A rat lung tissue according to claim 8 210 The method for rapid detection of Po nuclides is characterized by: In step S3, the method for measuring the count of alpha nuclides recovered by self-deposition is: The silver sheet was placed on an α spectrometer with the self-deposition side facing upwards and measured for 48 hours to obtain the α nuclide counts recovered by self-deposition and the α nuclide counts recovered by self-deposition were obtained respectively. 209 Po nuclide counting, recovery by autodeposition 210 Po nuclide counting.

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