A composition having the effect of promoting the expression of antioxidant enzymes and its application

Abstract

The present invention relates to the technical field of cosmetics, and discloses a composition having the effect of promoting the expression of antioxidant enzymes and its application. The composition having the effect of promoting the expression of antioxidant enzymes provided by the present invention comprises a turmeric root extract and dimethylmethoxychromanol, wherein the mass ratio of the turmeric root extract to the dimethylmethoxychromanol is (0.5-3):1. The present invention adopts turmeric root extract and dimethylmethoxychromanol to mix according to the mass ratio, and achieves a synergistic effect in promoting the expression of antioxidant enzymes. The composition having the effect of promoting the expression of antioxidant enzymes provided by the present invention is applied to cosmetics and / or skin care products, which can exert obvious skin antioxidant and anti-aging effects at a lower application amount.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to a composition having the function of promoting the expression of antioxidant enzymes and an application thereof. Background Art

[0002] During the metabolism of the body, when an electron of oxygen is taken away, the oxygen atom without a paired electron becomes a free radical. Free radicals are very unstable, highly oxidizing, and have a strong ability to absorb electrons. They need to take electrons from the cell molecules of body tissues to pair themselves. When a cell molecule replaces an electron, it also becomes a free radical and has to snatch electrons from the cell membrane or cell nucleus molecules, thus constantly generating new free radicals. Free radicals can cause protein denaturation and cross-linking, causing many enzymes and hormones in the body to lose their biological activity, reducing the vitality of the body's immune ability, nerve reflex ability, motor ability and other systems, and can also destroy the structure of nucleic acids and cause metabolic disorders in the entire body, ultimately causing the body to become diseased.

[0003] Under the action of enzymes, a considerable portion of the oxygen inhaled from the air is converted into superoxide anions, which are used to eliminate foreign invaders such as viruses and protect the body, while the excess superoxide anions become free radicals that cause human cells to age. The human body can produce endogenous antioxidants to catalyze the decomposition of superoxide anion free radicals, allowing the body to reach a safe and balanced state. However, as we age, the level of endogenous antioxidants produced by the human body will gradually decrease. At this time, the excess superoxide anion free radicals will attack the unsaturated phospholipids on the cell membrane, causing the cells to age prematurely, resulting in pigmentation and the formation of age spots, leading to human aging. Summary of the invention

[0004] The object of the present invention is to provide a composition having the effect of promoting the expression of antioxidant enzymes and its application.

[0005] To solve the above technical problems, the first aspect of the present invention provides a composition having the effect of promoting the expression of antioxidant enzymes, comprising a turmeric root extract and dimethylmethoxychromanol, wherein the mass ratio of the turmeric root extract to the dimethylmethoxychromanol is (0.5-3):1.

[0006] Preferably, in the composition having the efficacy of promoting the expression of antioxidant enzymes provided by the present invention, the mass ratio of the turmeric root extract to the dimethylmethoxychromanol is (1-2):1.

[0007] More preferably, in the composition having the efficacy of promoting the expression of antioxidant enzymes provided by the present invention, the mass ratio of the turmeric root extract to the dimethylmethoxychromanol is 1:1.

[0008] The second aspect of the present invention provides use of the composition having the function of promoting the expression of antioxidant enzymes according to the first aspect of the present invention in cosmetics and / or skin care products.

[0009] In the application provided by the present invention, the cosmetics and / or skin care products are applied to the outer surface of the skin.

[0010] Preferably, in the application provided by the present invention, the cosmetics and / or skin care products are selected from one or more of eye cream, face cream, essence, lotion, and mask essence.

[0011] Preferably, in the application provided by the present invention, the composition having the function of promoting the expression of antioxidant enzymes is dispersed in a matrix raw material as an additive, and the matrix raw material is selected from one or more of an oily raw material, a powdery raw material, and a gum raw material.

[0012] Preferably, in the application provided by the present invention, the cosmetics and / or skin care products further include: one or more of surfactants, flavors and fragrances, pigments, moisturizers, preservatives, antioxidants, ultraviolet absorbers, chelating agents, astringents, penetration aids, and nutritional additives.

[0013] Compared with the prior art, the present invention uses turmeric root extract and dimethylmethoxychromanol to mix according to a specific mass ratio, and achieves a synergistic effect in promoting the expression of antioxidant enzymes. Therefore, the composition with the effect of promoting the expression of antioxidant enzymes provided by the present invention is applied to cosmetics and / or skin care products, which can exert obvious skin antioxidant and anti-aging effects at a lower application amount. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 This is a comparison chart of the effects of blank control group, model group, single drug administration group 1, single drug administration group 2, and experimental group 1 on the activity of Hacat cell SOD induced by UVB;

[0015] Figure 2 This is a comparison chart of the effects of blank control group, model group, single drug administration group 1, single drug administration group 2, and experimental group 2 on the activity of Hacat cell SOD induced by UVB;

[0016] Figure 3 This is a comparison chart of the effects of blank control group, model group, single drug administration group 1, single drug administration group 2, and experimental group 3 on the activity of Hacat cell SOD induced by UVB;

[0017] Figure 4 WB images of the expression of NQO1 protein in Hacat cells induced by UVB in the blank control group, model group, single drug administration group 1, single drug administration group 2, and experimental group 1;

[0018] Figure 5 This is a comparison chart of the effects of blank control group, model group, single drug administration group 1, single drug administration group 2, and experimental group 1 on the expression of NQO1 protein in Hacat cells induced by UVB;

[0019] Figure 6 WB images of the expression of NQO1 protein in Hacat cells induced by UVB in the blank control group, model group, single drug administration group 1, single drug administration group 2, and experimental group 2;

[0020] Figure 7 This is a comparison chart of the effects of blank control group, model group, single drug administration group 1, single drug administration group 2, and experimental group 2 on the expression of NQO1 protein in Hacat cells induced by UVB;

[0021] Figure 8 WB images of the expression of NQO1 protein in Hacat cells induced by UVB in the blank control group, model group, single drug administration group 1, single drug administration group 2, and experimental group 3;

[0022] Fig. 9 This is a comparison chart of the effects of blank control group, model group, single drug administration group 1, single drug administration group 2, and experimental group 3 on UVB-induced Hacat cell NQO1 protein expression. DETAILED DESCRIPTION

[0023] To make the purpose, technical scheme and advantages of the present invention clearer, various embodiments of the present invention will be described in detail below. However, it will be appreciated by those skilled in the art that in various embodiments of the present invention, many technical details are provided to enable readers to better understand the present application. However, even without these technical details and various changes and modifications based on the following embodiments, the technical scheme claimed in the present application can be implemented.

[0024] An embodiment of the present invention provides a composition having the effect of promoting the expression of antioxidant enzymes, comprising a turmeric root extract and dimethylmethoxychromanol, wherein the mass ratio of the turmeric root extract to the dimethylmethoxychromanol is (0.5-3):1.

[0025] In some embodiments of the present invention, a composition having the effect of promoting the expression of antioxidant enzymes is provided, comprising a turmeric root extract and dimethylmethoxychromanol, wherein the mass ratio of the turmeric root extract to the dimethylmethoxychromanol is (1-2):1.

[0026] In some embodiments of the present invention, a composition having the effect of promoting the expression of antioxidant enzymes is provided, comprising a turmeric root extract and dimethylmethoxychromanol, wherein the mass ratio of the turmeric root extract to the dimethylmethoxychromanol is 1:1.

[0027] The composition having the function of promoting the expression of antioxidant enzymes provided by the embodiments of the present invention can be applied to cosmetics and / or skin care products, which are applied to the outer surface of the skin.

[0028] In some embodiments of the present invention, the cosmetics and / or skin care products using the composition having the function of promoting the expression of antioxidant enzymes are selected from one or more of eye cream, face cream, essence, lotion, and mask essence.

[0029] In some embodiments of the present invention, the composition having the function of promoting the expression of antioxidant enzymes is dispersed in a matrix raw material as an additive, and the matrix raw material is selected from one or more of an oily raw material, a powdery raw material, and a gum raw material.

[0030] The oily raw material can be any one or a combination of the following matrix raw materials: soybean oil, olive oil, almond oil, castor oil, peanut oil, cottonseed oil, tea seed oil, jojoba oil, avocado oil, coconut oil, palm oil, cocoa butter, rice bran oil, evening primrose oil, wheat germ oil, corn germ oil, palm wax, wood wax, mink oil, turtle oil, snake oil, egg yolk oil, beef tallow, horse tallow, pig tallow, deer tallow, lecithin, lanolin, beeswax, spermaceti, shellac wax, paraffin, vaseline, microcrystalline wax, ozokerite, squalane, lanolin wax, lanolin alcohol, lanolin Alcohol esters, acetylated lanolin, acetylated lanolin alcohol, lanolin acid, lanolin acid esters, polyoxyethylene lanolin, polyoxyethylene lanolin alcohol ether, polyoxypropylene lanolin alcohol ether, hydrogenated lanolin, alkoxylated hydrogenated lanolin, silicone oil (such as dimethyl silicone oil, octamethyl silicone oil, methylphenyl polysiloxane), polysiloxane-polyoxyalkyl block copolymers, methyl hydrogen silicone oil, Cn fatty acids and esters thereof, Cn fatty alcohols and esters thereof; wherein n≥10, and is preferably 10-24, more preferably 12-20, such as 14, 16, 17, 18.

[0031] The Cn fatty acids include lauric acid, myristic acid, palmitic acid, stearic acid, oleic acid, linoleic acid, erucic acid and capric acid.

[0032] The Cn fatty alcohols include: lauryl alcohol, cetyl alcohol, stearyl alcohol, isostearyl alcohol, oleyl alcohol, and coconut alcohol.

[0033] The Cn fatty acid esters and Cn fatty alcohol esters are such as: isopropyl myristate, myristyl myristate, myristyl lactate, hexadecanol lactate, isopropyl palmitate, palm tree isooctyl ester, butyl stearate, isooctyl stearate, monostearate glyceryl, polyethylene glycol stearate, decyl oleate, caprylic glyceryl, capric glyceryl, and triolein.

[0034] The powdery raw material can be any one or a combination of the following matrix raw materials: mica, magnesium carbonate, titanium dioxide, zinc white, calcium hydroxide, calcium carbonate, kaolin, calcium stearate, zinc stearate, magnesium stearate, talc, silicon dioxide, aluminum hydroxide, calcium pyrophosphate, calcium bicarbonate, and bentonite.

[0035] The oil raw material may be any one or more of plant oil raw materials, animal oil raw materials, mineral oil raw materials, synthetic oil raw materials, and semi-synthetic oil raw materials, such as starch, cyclodextrin, xanthan gum, carrageenan, guar gum, gum arabic, tragacanth gum, agar, shellac, sodium alginate, gelatin, methyl cellulose, ethyl cellulose, sodium carboxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, polycellulose ether quaternary ammonium salt, polyvinyl alcohol, polyvinyl pyrrolidone, sodium polyacrylate, polyoxyethylene, vinyl pyrrolidone / vinyl acetate copolymer, carbomer resin, magnesium aluminum silicate, and C12-15 alcohol benzoate.

[0036] The solvent raw material can be any one or a combination of the following matrix raw materials: water, ethanol, propanol, isopropanol, isobutanol, ethylene glycol, 1,2-propylene glycol, cyclohexane, dichlorodifluoromethane, tetrafluorodichloroethane, ethylene glycol monoethyl ether, diethylene glycol monoethyl ether, acetone, methyl isobutyl ketone, ethyl acetate, butyl acetate, amyl acetate, and dibutyl phthalate.

[0037] In some embodiments of the present invention, the cosmetics and / or skin care products using the composition having the function of promoting the expression of antioxidant enzymes also include: one or more of surfactants, flavors and fragrances, pigments, moisturizers, preservatives, antioxidants, ultraviolet absorbers, chelating agents, astringents, penetration aids, and nutritional additives.

[0038] Wherein, the surfactant can be: 1) anionic surfactant, such as: C12-14 fatty alcohol ammonium sulfate, C12-14 fatty alcohol polyoxyethylene ether carboxylate, dodecyl sulfate diethanolamine salt, C12-14 fatty alcohol polyoxyethylene ether ammonium sulfate, dodecyl sulfate triethanolamine, alcohol ether phthalic acid monoester sodium salt, acylated peptide, lauric acid monoethanolamide sodium sulfate, n-octanol polyoxypropylene ether succinic acid monoester sulfonate, hexadecanol succinic acid monoester sulfonate, sulfosuccinic acid monoester disodium salt, succinic acid monooctadecyl disodium salt, alcohol ether sulfosuccinic acid monoester disodium salt, fatty alcohol polyoxyethylene ether (sodium) sulfosuccinic acid monoester ammonium salt, oleamide sulfosuccinic acid monoester disodium salt, N - acyl glutamate potassium salt, sodium pyrrolidone carboxylate, sodium polyacrylate, fatty alcohol polyoxyethylene ether phosphate, fatty alcohol polyoxyethylene ether phosphate monoester and its salt, fatty alcohol polyoxyethylene ether phosphate monoester ethanolamine salt, alkylphenol polyoxyethylene ether phosphate monoester and its salt, nonylphenol ether phosphate monoester ethanolamine salt, alkyl phosphate ester salt, monolauryl phthalate salt, zinc undecylenate, anionic amino acid surfactant, sodium N-C12-18 acyl glutamate, sodium N-lauroyl L-alanine, monosodium N-stearoyl glutamate, monosodium N-cocoyl glutamate, monosodium N-mixed fatty acyl glutamate, sodium long straight chain alkyl aromatic ether sulfonate TH; 2) nonionic surfactant, Such as: oleyl alcohol polyoxyethylene ether (such as emulsifier VO series), alkylphenol polyoxyethylene ether (such as emulsifier OPE series), stearic acid polyoxyethylene ester, ethylene glycol monostearate, distearate ethylene glycol, polyethylene glycol monostearate, polyethylene glycol distearate, polyethylene glycol dilaurate, distearic acid glyceryl, tristearic acid glyceryl, sucrose stearate, polyoxyethylene glyceryl ether monostearate, N-lauroyl glutamic acid dialkyl alcohol ester, benzoic acid fatty alcohol ester, sorbitol monofatty acid ester (Span, Span, such as Span-20, Span-60, Span-65, Span-80, Span-83, Span-85), polyoxyethylene ether sorbitol monofatty acid ester (Tween, Tween en, such as Tween-20, Tween-40, Tween-60, Tween-61, Tween-80), diethanolamine, triethanolamine, fatty acid monoethanolamide (such as coconut acid monoethanolamide, lauric acid monoethanolamide, palmitic acid monoethanolamide), coconut acid diethanolamide, lauric acid diethanolamide, methyl glucoside stearate, polyoxyethylene methyl glucoside stearate, ethylene glycol glucoside stearate, propylene glycol glucoside stearate, C8-16 alkyl glucoside, glyceryl glucoside stearate, succinic acid lauryl ether monoester, lauryl glyceryl polyoxyethylene ether, stearic acid mannitol anhydride ester, polyoxyethylene mink oil, mink acid methyl ester, mink acid isopropyl;3) Cationic surfactants, such as C12-18 alkyl trialkyl ammonium chloride (such as hexadecyl dimethyl alkyl ammonium chloride, octadecyl trimethyl ammonium chloride, hexadecyl trimethyl ammonium chloride), oleamidopropyl dihydroxypropyl dimethyl ammonium chloride, dihydroxypropyl dimethyl dodecyl ammonium chloride, dimethyl diallyl ammonium chloride / acrylamide copolymer, oleamidopropyl-2,3-dihydroxypropyl dimethyl ammonium chloride, ethyl sulfate oleamidopropyl dimethyl ethyl ammonium, di-C12-18 dimethyl ammonium chloride, polyquaternium-11 conditioner, M-505 polyquaternium-7 conditioner, mink oil amide propylamine-chitosan, mink oil amide propylamine-hydrolyzed protein, cationic protein peptides, guar gum-hydroxypropyl trimethyl Ammonium chloride salt, polycellulose ether quaternary ammonium salt, triethanolamine monooleate, DNP series; 4) zwitterionic surfactants, such as C12-18 alkyl dihydroxyethyl betaine, N-C12-18 alkyl-N-(2-hydroxyethyl)-N-(2-formamidoethyl) ammonium acetate, C12-18 alkoxy hydroxypropyl betaine, cocamidopropylamine oxide, sodium coconut oil isethionate, octadecyl dimethylamine oxide, cocoyl diethanolamine oxide, N-alkyl-β-aminopropionyl diethanolamine, lauroyl propylamine oxide, N-C12-18 acyl glutamic acid, hydroxyethyl decanoate imidazoline betaine, hydroxyethyl myristate imidazoline betaine, hydroxyethyl palmitate imidazoline betaine. ;

[0039] Wherein, the essence and fragrance can be selected from: 1) natural fragrances, such as wormwood oil, eucalyptus oil, star anise oil, white lemon oil, white clove extract, clove oil, white orchid oil, white orchid extract, white orchid leaf oil, cedar oil, peppermint oil, atractylodes stearin, sweet osmanthus extract, orange leaf oil, clove basil oil, large flower jasmine extract, wintergreen oil, angelica oil, angelica extract, lichen acid, sandalwood oil, dahlia flower oil, dahlia leaf oil, hyacinth extract, maple extract, camphor oil, sassafras oil, spikenard oil, osmanthus extract, red tangerine oil, carrot seed oil, castoreum, sunflower oil, yellow orchid oil, patchouli oil, artemisia oil, osmanthus extract, acacia extract, ginger oil, orange oil, peppermint oil, chrysanthemum extract, cumin oil, ambergris, spearmint oil, civet, wintersweet oil, bell Orchid extract, costus root oil, Milan flower oil, rose geranium oil, rose oil, jasmine extract, oyster oil, black red extract, black red oil, lemon eucalyptus oil, lemon oil, seven li extract, nan leaf oil, celery seed oil, nutmeg oil, cinnamon oil, litsea cubeba oil, mink balm tincture, narcissus extract, pine needle oil, tree moss extract, jasmine extract, musk, muskrat balm, sweet orange oil, sandalwood oil, evening Fragrant jade oil, tuberose extract, marigold essential oil, fennel oil, blood cypress oil, citronella oil, geranium oil, carnation oil, perilla oil, clary perilla oil, cyperus oil, bergamot mint oil, vetiver oil, elsholtzia oil, elsholtzia extract, raspberry rose flower oil, raspberry rose flower extract, lavender oil, bay leaf oil, ylang ylang oil, coriander seed oil, fish vanilla oil, rock rose extract, rock cinnamon oil, grapefruit peel oil, iris oil, tobacco flower oil, citron leaf oil, gardenia extract, amorpha oil, violet leaf extract, guaiacol, 4-methylguaiacol, 4-ethylguaiacol, natural vanillin, camphor; 2) synthetic flavors, such as limonene, longifolene, ethyl caryophyllene, isolongifolene, isolongifolene, isolongifolene, bromostyraldehyde, diphenylmethane, diphenyl ether, m-methyl diphenyl ether, eugenol, isoeugenol, ethyl naphthyl methyl ether, ethyl naphthyl ethyl ether, p-methylanisole, isoeugenol benzyl ether, rose oxide, santalin ether, narcissin ether (or jasmine ether), ambroxan, methyl cedar ether, epoxy cedar, erinthal, erinthal N, 3-hexenol, decanol, lauryl alcohol, benzyl alcohol, phenylethyl alcohol, p-methoxybenzyl alcohol, cinnamyl alcohol, dimethylbenzyl alcohol, geraniol, nerol, linalool, rose alcohol, pinene alcohol, citronellol, racemic menthol, menthol, borneol, cedarwood alcohol, cedar alkane, methyl cedarwood ketone, 3,4-dioxymethylenebenzyl alcohol, α-methyl-3,4-dihydromethylenebenzyl propanal, 2-tert-butyl-4-methylcyclohexanol, clary alcohol, santalol, 3-methyl-5-(2',2',3'-trimethylcyclopenten-1'-yl)pentan-2-ol, benzaldehyde, acetophenone, phenylacetic acid, p-methylacetophenone benzophenone, lauryl aldehyde, cinnamaldehyde, cinnamic acid, α-pentyl-β-phenylpropionaldehyde, α-phenylpropionaldehyde, lily aldehyde, lyral, rabbit ear grass aldehyde, 3,4-dioxymethylenebenzaldehyde, 3,7-dimethyl-6-octenal, 3,7-Dimethyl-7-hydroxyoctanal, citral, ethyl-cyclohomocitral, ivinal, citrus aldehyde, 4-hydroxy-3-methoxybenzaldehyde, α-dihydrodamascenone, β-damascenone, ionone, 6-methylionone, nerolione, carvone, piperonyl acetone, raspberry ketone, herb ketone, cis-jasmone, heptaldehyde glycol acetal, phenylacetaldehyde dimethyl acetal, octanal dimethyl acetal, α-amylcinnamaldehyde dimethyl acetal, citral dimethyl acetal, citral diethyl acetal, anisealdehyde dimethyl acetal, mushroom aldehyde, chlorophyll, Hyacinthin, apple ester, gypsum ketal, geranyl formate, citronellyl formate, avermester, leaf acetate, benzyl acetate, phenylethyl acetate, cinnamyl acetate, pine acetate, bornyl acetate, linalyl acetate, geranyl acetate, p-tert-butylcyclohexyl acetate, o-tert-butylcyclohexyl acetate, bergamyl acetate, succinate acetate, avermester acetate, decahydronaphthyl acetate, cedar acetate, geranyl propionate, trichloromethylbenzyl acetate, benzyl butyrate, leaf butyrate, geranyl butyrate, 2-methyl phenyl valeric acid Esters, isobutyric acid p-cresyl ester, 2-nonenoic acid methyl ester, ethyl benzoate, methyl benzoate, benzoic acid leaf alcohol ester, benzyl benzoate, ethyl phenylacetate, phenylacetic acid p-cresyl ester, ethyl cinnamate, butyl salicylate, isobutyl salicylate, isopentyl salicylate, salicylic acid leaf alcohol ester, hexyl salicylate, benzyl salicylate, phenylethyl salicylate, cyclohexyl salicylate, jasmonate, jasmonate, 2-pentylcyclopentanone acetate methyl ester, nerolidin, γ-nonalactone, γ-undecalactone, coumarin, indole, 3-methylindole, 3,7- Dimethyl-2,6-octadienenitrile, p-methoxybenzonitrile, xylene musk, ketone musk, 2,6-dinitro-3-methoxy-4-tert-butyltoluene, 1,1-dimethyl-4-acetyl-6-tert-butylindane, hexamethyltricyclic isochroman musk, 1,1,2,3,3,6-hexamethyl-5-acetylindane, musk-T, musk-M, musk-L, musk-F, 11-oxacyclohexadecanolide, tonaflavone musk; 3) essence, rose essence, jasmine essence, sandalwood essence, white orchid essence.

[0040] Wherein, the pigment can be one or more selected from the following components: zirconium dioxide, lead acetate, silver nitrate, chlorophyll, copper chlorophyll, ferric ferrocyanide, guanine, fast red, food red I, vat red I, carmine, edible bayberry red, edible cherry red, food red 17, amaranth, fast green, food yellow 3, edible lemon yellow, edible Sudan yellow, edible indigo, food blue 2, fluorescent pink, β-carotene, henna, gardenia red pigment, gardenia green pigment, gardenia blue pigment, gardenia yellow pigment, titanium mica pearlescent pigment, red iron oxide, yellow iron oxide, black iron oxide, chromium oxide green, bismuth oxychloride, basic pink, silver vermilion R, quinoline yellow, lithophane, lac red pigment, ultramarine, acid red 87, acid green 25, acid fluorescent yellow, acid orange 7, golden red 53, lithotripsy BK, lithotripsy 2R, and permanent orange.

[0041] Among them, the preservative can be one or a combination selected from the following components: sorbic acid and its salts, propionates, parahydroxybenzoates, benzyl alcohol, benzoic acid and its salts, 5-chloro-2-methyl-4-isothiazoline-3-one, -2-methyl-4-isothiazoline-3-one, kasone, imidazolidinyl urea, dehydroacetic acid and its salts, sodium sulfite, sodium bisulfite, sodium pyrosulfite, 2,2-bromo-2-nitro-1,3-propanediol (Bropol), isoascorbic acid and its salts, kojic acid and its esters, azelaic acid, usnic acid, and resorcinol.

[0042] The antioxidant may be one or a combination of the following components: SOD, butylated hydroxyanisole, gallic acid ester, ascorbic acid and its salts, isoascorbic acid and its salts, sitosterol, rutin, and tocopherol.

[0043] Among them, the moisturizing agent can be one or a combination selected from the following components: 1,2-butylene glycol, sorbitol, xylitol, glycerol, lactate, polyethylene glycol, DL-pyrrolidone carboxylate, D-glucose, kojic acid and its esters, uric acid, orotic acid, pectinic acid, laminin, collagen, trehalose, 12, and cholesterol hydroxystearate.

[0044] Among them, the ultraviolet absorber can be one or a combination selected from the following components: pyridoxine hydrochloride, kojic acid and its esters, rutin, aloin, caffeine, hesperetin, 2-ethylhexyl salicylate, 2-ethylhexyl p-dimethylaminobenzoate, 2-ethylhexyl p-methoxycinnamate, phenyl salicylate, anthranilate, 2-hydroxy-4-methoxybenzophenone, 2-hydroxy-4-methoxybenzophenone-5-sulfonic acid, and 2-hydroxy-4-octyloxybenzophenone.

[0045] Wherein, the chelating agent can be one or a combination of the following components: EDTA disodium salt, bispyrithione zinc, sodium thiosulfate.

[0046] Wherein, the astringent can be one or a combination of the following components: zinc phenol sulfonate, hydroxyaluminum chloride, aluminum sulfate, potassium aluminum sulfate, zinc sulfate, aluminum chloride, zinc chloride, and calamine.

[0047] Wherein, the penetration enhancer can be one or a combination of the following components: dimethyl sulfoxide, 1-n-dodecylazacycloheptan-2-one, and 2-aminoethanol.

[0048] Wherein, the pH adjuster can be one or a combination of the following components: tartaric acid, lactic acid, boric acid, and succinic acid.

[0049] The nutritional additives may be one or a combination of the following components: chitin, chitosan, silk peptide, silk fibroin, lecithin, epidermal growth factor, trehalose, lactose, pearl powder, collagen, collagen hydrolyzate, hyaluronic acid and its salts, egg membrane element, phytosterol, lysozyme chloride, sodium chondroitin sulfate, propolis, propolis, honey, glycyrrhizic acid, glycyrrhetinic acid, γ-linolenic acid, kojic acid and its esters, gibberellic acid, glycine, L-aspartic acid and its salts, L-cysteine ​​and its salts, DL-alanine, L-serine, DL-silk Amine, L-methionine, DL-methionine, L-lysine hydrochloride, L-threonine, inositol, maltol, pantothenate, D-panthenol, retinyl acetate, allantoin, pyridoxine hydrochloride, cortisone, niacinamide, vitamins, estrogen, hematoxylin, uric acid, orotic acid, aloin, azelaic acid, rosin acid, usnic acid, laminin, thymosin, madder, limonene, baicalin, deoxycholic acid, guaiac, hesperidin, tannic acid, selenium disulfide, tea saponin, triethyl citrate, butylated hydroxytoluene, phytic acid, polydimethyldiallylammonium chloride.

[0050] Experimental example

[0051] The instruments, kits, cells and reagents used in the experimental examples can all be purchased through public commercial channels and are commonly used instruments, kits, cells and reagents in the field. The operating procedures of the instruments and kits are carried out according to the instructions of their instructions.

[0052] 1. Purpose of the experiment

[0053] A UVB-induced oxidative damage model of Hacat cells was established, and the SOD detection kit was used to detect the effects of cosmetic raw materials on the expression levels of antioxidant enzymes. Western Blot was used to detect the effects of cosmetic raw materials on the expression levels of NQO1 protein.

[0054] Superoxide dismutase (SOD) is an important antioxidant enzyme in organisms that catalyzes the dismutation of superoxide anions to generate hydrogen peroxide (H2O2) and oxygen (O2). NQO1 oxidoreductase 1 (NQO1) is a homodimeric flavoenzyme that can prevent environmental stressors from causing oxidative damage to DNA. In addition, NQO1 plays an important role in protecting endogenous antioxidants by maintaining the reduced forms of ubiquinone and α-tocopherol quinone.

[0055] 2. Experimental Materials

[0056] Ingredients 1:

[0057] Name: Turmeric root extract; Trade name: Tego Turmerone; Properties: oily; Purchased from Evonik Specialty Chemicals (Shanghai) Co., Ltd.

[0058] Ingredients 2:

[0059] Name: Dimethylmethoxychromanol; Trade name: LIPOCHROMAN; Properties: light brown to brown powder; Purchased from Lubrizol Management (Shanghai) Co., Ltd.

[0060] 3. Main instruments and reagents

[0061] Table 1. Main instruments used in the experiment

[0062]

[0063]

[0064] Table 2. Main reagents used in the experiment

[0065]

[0066] 4. Cell Culture

[0067] Cell type: Hacat immortalized human keratinocytes.

[0068] Source: Cell Bank of Wuhan University, Committee of Type Culture Collection, Chinese Academy of Sciences.

[0069] Cell Type: Adherent cells.

[0070] Culture conditions: 5% CO2, 37°C constant temperature incubator.

[0071] Culture medium: 89% MEM + 10% FBS + 1% double antibody.

[0072] 5. Preparation of test and reference substances

[0073] The test substances and control substances were prepared into stock solutions with DMSO or ddH2O, and then diluted to appropriate concentrations with serum-free culture medium or PBS to form the following experimental groups:

[0074] Table 3 Experimental groups

[0075]

[0076]

[0077] 6. Test methods

[0078] (I) Antioxidant enzyme detection

[0079] 1. Detection steps

[0080] Cells in the exponential growth phase were prepared into single-cell suspensions at a concentration of 1.5 × 10 5Cells were inoculated at a density of 100 cells / mL on a 6-well plate and cultured at 37°C for 24 hours in a medium containing 10% fetal bovine serum to allow the cells to adhere to the wall. The final culture volume was 2 mL. The supernatant was then discarded and serum-free medium or freshly prepared serum-free medium containing different concentrations of the test substance was added for incubation. The final culture volume was 2 mL. After culturing for 12 hours / 16 hours / 24 hours / 32 hours / 48 hours / 72 hours, the cells were irradiated with UVB (50 J / cm 2 , 5min), and after culturing at 37℃ for 16h, the SOD activity was detected using the Biyuntian total SOD activity detection kit.

[0081] The Total Superoxide Dismutase Assay Kit with WST-8 of Bio-Tech is a kit based on the colorimetric reaction of WST-8 to detect the activity of SOD, i.e. superoxide dismutase, in cells, tissues or other samples by colorimetry. The specific operation steps are carried out according to the instructions of the kit.

[0082] 2. Data entry and statistical analysis:

[0083] The data were input into Excel and analyzed by Prism (Version 7) software package, and the data were expressed as mean ± standard error. One-way analysis of variance was performed between the groups, and p < 0.05 was considered statistically significant.

[0084] 3. Experimental results

[0085] Figure 1 This is a comparison chart of the effects of blank control group, model group, single drug administration group 1, single drug administration group 2, and experimental group 1 on the activity of Hacat cell SOD induced by UVB;

[0086] Figure 2 This is a comparison chart of the effects of blank control group, model group, single drug administration group 1, single drug administration group 2, and experimental group 2 on the activity of Hacat cell SOD induced by UVB;

[0087] Figure 3 This is a comparison chart of the effects of the blank control group, model group, single drug administration group 1, single drug administration group 2, and experimental group 3 on UVB-induced Hacat cell SOD activity.

[0088] Figure 1 Middle: n=3, Mean±SEM. “###” indicates p<0.001 compared with the control group, “*” indicates p<0.05 compared with the model group, “**” indicates p<0.01 compared with the model group, and “***” indicates p<0.001 compared with the model group.

[0089] according to Figure 1, the experimental group 1 was administered (calculated by mass, turmeric root extract: lipochroman = 1:1). Compared with the single-administration group 1 (lipochroman alone) and the single-administration group 2 (turmeric root extract alone), the experimental group 1 could significantly increase the SOD of Hacat cells, indicating that the experimental group had a synergistic effect in promoting the expression of antioxidant enzymes.

[0090] according to Figure 2 , experimental group 2 (calculated by mass, turmeric root extract: lipochroman = 2:1) was used for administration. Compared with group 1 (lipochroman alone) and group 2 (turmeric root extract alone), experimental group 2 could also significantly increase the SOD activity of Hacat cells.

[0091] according to Figure 3 , Experimental group 3 was administered (calculated by mass, turmeric root extract: lipochroman = 1:5). Compared with group 1 (lipochroman alone) and group 2 (turmeric root extract alone), experimental group 3 had no significant effect in increasing the SOD activity of Hacat cells.

[0092] (II) Detection of NQO1 protein expression level

[0093] 1. Detection steps

[0094] Cells in the exponential growth phase were prepared into single-cell suspensions at a concentration of 1.5 × 10 5 Cells were inoculated at a density of 100 cells / mL on a 6-well plate and cultured at 37°C for 24 hours in a medium containing 10% fetal bovine serum to allow the cells to adhere to the wall. The final culture volume was 2 mL. The supernatant was then discarded and serum-free medium or freshly prepared serum-free medium containing different concentrations of the test substance was added for incubation. The final culture volume was 2 mL. After culturing for 12 hours / 16 hours / 24 hours / 32 hours / 48 hours / 72 hours, the cells were irradiated with UVB (50 J / cm 2 , 5 min), and after incubation at 37°C for 16 h, the NQO1 protein content was detected by Western Blot.

[0095] Method for extracting total protein: add lysis buffer to a 6-well plate, lyse on ice for 30 minutes, scrape the cells with a cell scraper and transfer to a centrifuge tube. Centrifuge at 4°C, 12,000 rpm for 5 minutes, take the supernatant and place it in a new centrifuge tube, add 5× loading buffer according to the volume of the supernatant, and boil it in boiling water for 10 minutes to denature the protein. After cooling, determine the protein concentration and store it at -80°C.

[0096] BCA method for protein concentration determination: The operation steps are in accordance with the kit instructions. (BCA kit brand: Biosharp)

[0097] Western blot (NQO1 antibody, CST): The protein was loaded for SDS-PAGE gel electrophoresis. After electrophoresis, the protein bands were transferred to the NC membrane, blocked with 5% skim milk, incubated with the primary antibody at 4°C overnight, washed, and incubated with the secondary antibody for 1 hour. Finally, the bands were developed using ECL substrate luminescent solution.

[0098] 2. Data entry and statistical analysis:

[0099] The data were input into Excel and analyzed by Prism (Version 7) software package, and the data were expressed as mean ± standard error. One-way analysis of variance was performed between the groups, and p < 0.05 was considered statistically significant.

[0100] 3. Experimental results

[0101] Figure 4 WB images of NQO1 protein expression in blank control group, model group, single drug administration group 1, single drug administration group 2, and experimental group 1 after administration of UVB-induced Hacat cells;

[0102] Figure 5 This is a comparison chart of the effects of blank control group, model group, single drug administration group 1, single drug administration group 2, and experimental group 1 on the expression of NQO1 protein in Hacat cells induced by UVB;

[0103] Figure 6 WB images of NQO1 protein expression in blank control group, model group, single drug administration group 1, single drug administration group 2, and experimental group 2 after administration of UVB-induced Hacat cells;

[0104] Figure 7 This is a comparison chart of the effects of blank control group, model group, single drug administration group 1, single drug administration group 2, and experimental group 2 on the expression of NQO1 protein in Hacat cells induced by UVB;

[0105] Figure 8 WB images of NQO1 protein expression in blank control group, model group, single drug administration group 1, single drug administration group 2, and experimental group 3 after administration of UVB-induced Hacat cells;

[0106] Fig. 9 This is a comparison chart of the effects of blank control group, model group, single drug administration group 1, single drug administration group 2, and experimental group 3 on UVB-induced Hacat cell NQO1 protein expression.

[0107] Wherein, n=3, Mean±SEM. “#” indicates p<0.05 compared with the control group, “##” indicates p<0.01 compared with the control group, and “*” indicates p<0.05 compared with the model group.

[0108] according to Figure 4 and Figure 5 The experimental group 1 (calculated by mass, turmeric root extract: lipochroman = 1:1) was used for administration. Compared with the single-administration group 1 (lipochroman alone) and the single-administration group 2 (turmeric root extract alone), the experimental group 1 could significantly increase the NQO1 protein expression level of Hacat cells.

[0109] according to Figure 6 and Figure 7 , experimental group 2 was administered (calculated by mass, turmeric root extract: lipochroman = 1:5). Compared with group 1 (lipochroman alone) and group 2 (turmeric root extract alone), experimental group 2 had no significant effect in increasing the expression level of NQO1 protein in Hacat cells.

[0110] according to Figure 8 and Fig. 9 , experimental group 3 was used for administration (by mass: Et-VC: VC-IP: VE: NAC = 2:3:0.5:0.01). Compared with group 1 (lipochroman alone) and group 2 (turmeric root extract alone), experimental group 3 had no significant effect in increasing the NQO1 protein expression level of Hacat cells.

[0111] Those skilled in the art will appreciate that the above-mentioned embodiments are specific examples for implementing the present invention, and in actual applications, various changes may be made thereto in form and detail without departing from the spirit and scope of the present invention.

Claims

1. A composition having the effect of promoting the expression of antioxidant enzymes, characterized in that: The invention is composed of turmeric root extract and dimethylmethoxychromanol, wherein the mass ratio of the turmeric root extract to the dimethylmethoxychromanol is 1:1, and the composition promotes the expression of SOD and NQO1.

2. Use of the composition having the effect of promoting the expression of antioxidant enzymes according to claim 1 in the preparation of cosmetics and / or skin care products.

3. The use according to claim 2, characterized in that: The cosmetic and / or skin care product is applied to the outer surface of the skin.

4. The use according to claim 2, characterized in that: The cosmetics and / or skin care products are selected from one or more of eye cream, face cream, essence, and lotion.

5. The use according to claim 4, characterized in that: The essence is a facial mask essence.

6. The use according to claim 2, characterized in that: The composition having the function of promoting the expression of antioxidant enzymes is dispersed in a matrix raw material as an additive, and the matrix raw material is selected from one or more of an oily raw material, a powdery raw material, and a colloid raw material.

7. The use according to claim 2, characterized in that: The cosmetics and / or skin care products may also include: one or more of surfactants, flavors and fragrances, pigments, moisturizers, preservatives, antioxidants, ultraviolet absorbers, chelating agents, astringents, penetration aids, and nutritional additives.

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