Application of Chinese wolfberry fruit puree in preparation of anti-aging preparation

Abstract

The invention relates to the technical field of health-care food, in particular to application of Chinese wolfberry fruit puree in preparation of an anti-aging preparation. The invention provides application of Chinese wolfberry fruit puree in preparation of an anti-aging preparation. The Chinese wolfberry fruit puree comprises Chinese wolfberry fruits and Chinese wolfberry fruit seed oil extracted from Chinese wolfberry fruit seeds. According to the present invention, the Chinese wolfberry primary pulp can significantly enhance the muscle strength of mice, improve the movement coordination degree and the balance force of the mice, and achieve the effect better than the effect of the single use of the same dose of the Chinese wolfberry aqueous solution, such that the Chinese wolfberry primary pulp has the good anti-aging effect;

Description

Technical Field

[0001] This invention relates to the field of health food technology, and in particular to the application of wolfberry pulp in the preparation of anti-aging agents. Background Technology

[0002] Biologically, aging is a spontaneous and inevitable process that occurs naturally over time. It is a complex natural phenomenon characterized by structural degeneration, functional decline, and reduced adaptability and resistance. Physiologically, aging is viewed as the individual's developmental history, beginning with fertilization and continuing into old age. Pathologically, aging results from stress and strain, injury and infection, weakened immune responses, nutritional imbalances, metabolic disorders, and the accumulation of neglect and misuse of drugs. In short, aging is the inevitable outcome of the combined effects of many pathological, physiological, and psychological processes; it is a process of loss and degeneration of the body's constituent substances, tissue structure, and physiological functions. With an aging population, the incidence of age-related chronic diseases, such as Parkinson's disease (PD), Alzheimer's disease (AD), and malignant tumors, continues to rise, seriously affecting human health and quality of life. Therefore, slowing down the aging process has become a major focus in the exploration of the mysteries of life.

[0003] Goji berries are a plant used both as food and medicine. They are sweet and neutral in nature, and enter the liver and kidney meridians. They nourish the liver and kidneys, benefit essence and improve eyesight, and have high medicinal value. Goji berries are rich in carotene, vitamins A, B1, B2, C, and essential nutrients for eye health such as calcium and iron, earning them the nickname "bright eye berries." Furthermore, the betaine they contain can inhibit fat deposition in liver cells and promote liver cell regeneration, thus protecting the liver. Patent (CN202411303649) discloses a traditional Chinese medicine composition containing goji berries that can prolong telomeres in aged mice or delay their shortening, helping to slow the aging process and extend lifespan.

[0004] The wolfberry puree is exclusively developed by the Science and Technology Innovation Research Institute under Gansu Pharmaceutical Group. It is made from fresh wolfberries and wolfberry seed oil in an appropriate ratio, and is a dual-purpose food and medicine product. It has the effects of supplementing nutrition and nourishing the liver and kidneys, and can be used for conditions such as liver and kidney yin deficiency and insufficient essence and blood. This invention discloses a new use for wolfberry puree. Summary of the Invention

[0005] The purpose of this invention is to provide a new use for wolfberry juice in the preparation of anti-aging agents.

[0006] To achieve the above-mentioned objectives, the present invention provides the following technical solution: This invention provides the application of wolfberry puree in the preparation of anti-aging agents, wherein the components of the wolfberry puree include wolfberry and wolfberry seed oil extracted from wolfberry seeds.

[0007] Preferably, the wolfberry pulp also includes food- and / or pharmaceutically acceptable excipients.

[0008] Preferably, the dosage form of the preparation includes tablets, sprays, granules, capsules, oral liquids, and injections.

[0009] Beneficial effects: This invention provides a novel use of wolfberry juice in the preparation of anti-aging agents. Specifically, the aging is an doxorubicin-induced aging model. The wolfberry juice can significantly enhance the muscle strength of mice and improve their motor coordination and balance, achieving better results than using the same dose of wolfberry aqueous solution alone. This indicates that wolfberry juice has a better anti-aging effect and is expected to be used in clinical applications. Attached Figure Description

[0010] Figure 1 These are the results of a mouse grip strength test. Figure 2 These are the results of the mouse pole climbing test; Figure 3 These are the test results from a rotating bar fatigue tester. Detailed Implementation

[0011] This invention provides the application of wolfberry puree in the preparation of anti-aging agents, wherein the components of the wolfberry puree include wolfberry and wolfberry seed oil extracted from wolfberry seeds.

[0012] In this invention, the wolfberry pulp also includes food- and / or pharmaceutically acceptable excipients.

[0013] In this invention, the dosage forms of the preparation include tablets, sprays, granules, capsules, oral liquids, and injections.

[0014] The technical solutions provided by the present invention will be described in detail below with reference to the embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0015] Example 1

[0016] Twenty-four clean-grade male Kunming mice (four weeks old, weighing 22±2g, from the Experimental Animal Building of Lanzhou University) were acclimatized for one week under conditions of 20-25℃ and a regular 12h / 12h day / night cycle. Then, they were randomly divided into four groups: control group (Control), model control group (Model), wolfberry group (M+GQ), and wolfberry juice group (M+GQJ), with six mice in each group. Mice in the control group and model control group were fed physiological saline; 3% wolfberry aqueous solution was diluted with water at a volume ratio of 1:20 in the drinking water group; wolfberry puree group was diluted with wolfberry puree with water at a volume ratio of 1:20 in the drinking water group; mice were allowed free access to water. The preparation method of the 3% wolfberry aqueous solution is as follows: place wolfberry slices in a 37℃ oven to dry, grind them into powder with a grinder, and then dissolve them with physiological saline to prepare a 3% wolfberry aqueous solution (prepare and use immediately); wolfberry puree (purchased from Gansu Pharmaceutical Group Co., Ltd.) was developed by the Science and Technology Innovation Research Institute under Gansu Pharmaceutical Group. The components of wolfberry puree include wolfberry and wolfberry seed oil extracted from wolfberry seeds; After one week of feeding, except for the blank control group mice, the other groups of mice were intraperitoneally injected with doxorubicin (purchased from Shanghai Mairui Biochemical Technology Co., Ltd., CAS: 25316-40-9) at a dose of 3 mg / kg, once every three days, for a total of 5 injections. The blank control group mice were injected with the same dose of physiological saline. Except for the day of doxorubicin injection, the mice were fed normally with 3% wolfberry aqueous solution and wolfberry juice every day. The blank control group and model group mice had free access to water until the last dose of doxorubicin was administered.

[0017] Behavioral tests were performed on mice after they were injected with doxorubicin, focusing on motor and cognitive aspects: (1) Grip test Following the fourth dose of doxorubicin, all mice underwent a grip strength test: Mice were placed at the measurement location of the grip strength meter, trained to grasp the measuring grid with all four limbs, and their maximum reading at the moment of force application was recorded; this reading represented the animal's grip strength. Three, but not consecutive, measurements were taken, and the average was calculated to obtain more stable and reliable data for assessing the mice's muscle strength. The results are as follows: Figure 1 As shown; The results showed that, compared with the blank control group, the model group mice had weakened muscle strength, consistent with the doxorubicin-induced aging model, indicating that the model was successfully constructed. p <0.001). Compared with the model group, both wolfberry juice and wolfberry significantly enhanced the muscle strength of mice. p <0.05); and the effect of wolfberry juice in improving the grip strength and muscle strength of mice is better than that of wolfberry alone; wolfberry juice has the effect of reversing the muscle weakness induced by doxorubicin in aging mouse models.

[0018] (2) Pole climbing test

[0019] The pole climbing test is an essential tool for studying animal movement and balance. It is a simple, non-invasive method that aims to assess the limb movement or muscle function of rodents. After the fifth dose of doxorubicin, all mice underwent a pole-climbing test. During the test, the mice were placed at the top of the pole with their heads down, and the time it took for the mice to climb from the top to the bottom (with all four paws on the ground) was recorded. Each mouse was tested three times, but not consecutively. The results are as follows: Figure 2 As shown; The results showed that, compared with the blank control group, the time taken for mice in the model group to climb from the top of the pole to the bottom was significantly longer. p <0.01 indicates that doxorubicin-induced aging mice exhibited slower reaction times, reduced limb coordination, and weakened motor function, indicating successful model establishment. Compared to the model group, both wolfberry juice and wolfberry improved motor function and limb coordination in aging mice and shortened pole-climbing time. However, the time taken for mice in the wolfberry juice group to climb the pole was significantly shorter than that in the model group. p <0.05), wolfberry juice can improve motor coordination and limb flexibility in aging mice, and the effect is better than using wolfberry alone.

[0020] (3) Rotary bar fatigue test

[0021] The swivel test is used to assess the motor coordination and balance of rodents: the animal must maintain balance on a rotating bar, and the instrument records the time required for the animal to fall from the bar at different speeds or at continuously accelerating speeds, the speed of the bar when it falls, and the distance the animal travels. After the fifth dose of doxorubicin, all mice underwent a rotarod fatigue test. One mouse was placed in each lane, and after all mice on the bar were kept balanced, the start button was pressed to begin the test. The bar initially rotated at a constant speed of 5 rpm, gradually accelerating from 5 rpm to 30 rpm over 60 seconds. The time it took for each mouse to fall off the bar was recorded. Each mouse was tested three times, but not consecutively. The results are as follows: Figure 3 As shown; The results showed that, compared with the blank control group, the model group mice had a shorter time spent on the rotating rod, but both wolfberry juice and wolfberry could prolong the time the mice stayed on the rotating rod.

[0022] As can be seen from the above embodiments, the present invention provides the application of wolfberry juice in the preparation of anti-aging agents.

[0023] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. The application of wolfberry puree in the preparation of anti-aging agents, wherein the components of the wolfberry puree include wolfberry and wolfberry seed oil extracted from wolfberry seeds.

2. The application according to claim 1, characterized in that, The goji berry juice also includes food- and / or pharmaceutically acceptable excipients.

3. The application according to claim 1, characterized in that, The dosage forms of the preparations include tablets, sprays, granules, capsules, oral liquids, and injections.

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