Immuno-microfluidic chip and application thereof

Abstract

The application discloses an immune micro-fluidic chip and application thereof, which comprises a micro-fluidic chip body, a puncture part and a liquid capsule part, wherein the micro-fluidic chip body is provided with a puncture cavity and a reaction cavity, the puncture cavity is communicated with the reaction cavity through a connecting channel 103, the puncture cavity is provided with the puncture part, the puncture cavity is connected with the liquid capsule part, and the liquid capsule part has elasticity; when the liquid capsule part is pressed to contact the puncture part, the puncture part can puncture the liquid capsule part to release the liquid in the liquid capsule part. The immune micro-fluidic chip integrates the bag capsule for storing liquid on the micro-fluidic chip, releases the liquid by using external force, avoids manual adding procedures, simplifies operation steps, reduces reagent pollution, reduces experimental errors caused by manual operation, and is expected to be widely applied in micro-fluidic chip systems driven by external force.

Description

Technical Field

[0001] This application relates to the field of biochemical technology, and in particular to an immune microfluidic chip and its applications. Background Technology

[0002] Microfluidic chips are microanalytical systems that integrate sample pretreatment, mixing, reaction, separation, and detection into one or more chips, replacing traditional laboratory procedures. Microfluidic chips offer advantages such as small sample volumes, simple operation, and the ability to accurately complete the entire process from sample preparation to result display in a short time, effectively overcoming experimental errors caused by manual operations in traditional laboratory work. Therefore, microfluidic chips are increasingly used in fields such as chemical analysis, DNA sequencing, protein analysis, single-cell analysis, single-molecule analysis, food safety, environmental monitoring, and drug screening. For example, current immunoassay microfluidic chips all employ enzyme-catalyzed chemiluminescence detection or enzyme-linked immunosorbent assay (ELISA) methods.

[0003] Traditional microfluidic chip sampling methods typically involve manual sampling or instrument-based reagent needle sampling. Manual sampling is not only cumbersome but also prone to unavoidable reagent contamination, leading to errors in experimental results. More importantly, it cannot achieve in-situ sampling and therefore cannot utilize more advanced direct chemiluminescence. Instrument-based reagent needle sampling requires a complex liquid path system to handle sampling and cleaning, generating waste liquid and introducing contaminants from the reagent needle itself. Summary of the Invention

[0004] Based on this, in order to address at least one of the following problems in traditional technology, manual sample addition is not only cumbersome and may lead to unavoidable reagent contamination, resulting in errors in experimental results, and the inability to achieve in-situ sample addition, thus preventing the use of more advanced direct chemiluminescence, as well as the need for a complex liquid circuit system to meet the requirements of sample addition and cleaning for instrument reagent needles, which not only generates waste liquid but also causes contamination from the reagent needles, it is necessary to provide an immunomicrofluidic chip.

[0005] One embodiment of this application provides an immune microfluidic chip.

[0006] An immunomicrofluidic chip includes a microfluidic chip body, a puncture component, and a liquid sac component. The microfluidic chip body has a puncture chamber and a reaction chamber. The puncture chamber is connected to the reaction chamber through a connecting channel. The puncture component is disposed in the puncture chamber. The liquid sac component is connected to the puncture chamber. The liquid sac component is elastic. When the liquid sac component is pressed until it contacts the puncture component, the puncture component can puncture the liquid sac component to release the liquid inside the liquid sac component.

[0007] In some embodiments, the microfluidic chip body is provided with a plurality of puncture chambers and a plurality of reaction chambers, one or more of the puncture chambers are connected to one or more of the reaction chambers through connecting channels, and each of the puncture chambers is respectively connected to the liquid bladder component.

[0008] In some embodiments, the outer periphery of the fluid bladder component is sealed to the inner wall of the puncture cavity.

[0009] In some embodiments, the liquid bladder component is a plastic liquid bladder, an aluminum foil liquid bladder, or an aluminum composite liquid bladder.

[0010] In some embodiments, the fluid-filled bladder component is fixed to the puncture cavity by means of bonding, laser processing, heat sealing, or ultrasonic welding.

[0011] In some embodiments, the puncture component is a columnar structure.

[0012] In some embodiments, the end of the puncture member facing the fluid sac member has a pointed structure.

[0013] In some embodiments, the puncture component is one or more of the following shapes: needle-shaped, serrated, and cross-shaped.

[0014] In some embodiments, the reaction chamber contains magnetic beads and microspheres to achieve solid-phase deposition of antigens and antibodies, thereby enabling the separation of immune complexes.

[0015] In some embodiments, the reaction chamber has a microporous structure to achieve solid-phase deposition of antigens and antibodies, thereby enabling the separation of immune complexes.

[0016] The aforementioned immunomicrofluidic chips have applications in chemical analysis, DNA sequencing, protein analysis, single-cell analysis, single-molecule analysis, food safety, environmental monitoring, and drug screening.

[0017] The immunomicrofluidic chip of the present invention integrates a storage sac for liquid on the microfluidic chip and releases the liquid by means of external force, avoiding the manual sample addition procedure, simplifying the operation steps, reducing reagent contamination, and reducing experimental errors caused by manual operation. It is expected to be widely used in microfluidic chip systems that use external force as the driving force.

[0018] The columnar structure of the aforementioned immunomicrofluidic chip facilitates the user's puncture of the bladder component. During use, simply press the bladder component gently into the puncture cavity. Once the columnar structure contacts the bladder component, the puncture can be performed, releasing the liquid inside.

[0019] In the aforementioned immunomicrofluidic chip, the number of puncture chambers and the number of reaction chambers can be set according to actual needs. When there are multiple puncture chambers and multiple reaction chambers, the connectivity between them can be configured to achieve connectivity between one puncture chamber and multiple reaction chambers, or between multiple puncture chambers and one reaction chamber, or even between multiple puncture chambers and multiple reaction chambers.

[0020] In the aforementioned immunomicrofluidic chip, the columnar structure facilitates the user's puncture of the bladder component. During use, simply press the bladder component gently toward the puncture cavity. Once the columnar structure contacts the bladder component, the puncture can be performed, releasing the liquid inside the bladder component. Attached Figure Description

[0021] To more clearly illustrate the technical solutions in the embodiments of this application, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the accompanying drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0022] To gain a more complete understanding of this application and its beneficial effects, the following description will be provided in conjunction with the accompanying drawings. In the following description, the same reference numerals denote the same parts.

[0023] Figure 1 This is a schematic diagram of an immune microfluidic chip according to an embodiment of the present invention.

[0024] Explanation of reference numerals in the attached figures

[0025] 10. Immunofluidic chip; 100. Microfluidic chip body; 101. Puncture chamber; 102. Reaction chamber; 103. Connection channel; 200. Puncture component; 300. Fluid sac component. Detailed Implementation

[0026] To make the above-mentioned objects, features, and advantages of the present invention more apparent and understandable, specific embodiments of the present invention will be described in detail below with reference to the accompanying drawings. Many specific details are set forth in the following description to provide a thorough understanding of the present invention. However, the present invention can be practiced in many other ways different from those described herein, and those skilled in the art can make similar modifications without departing from the spirit of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0027] In the description of this invention, it should be understood that the terms "center," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," and "circumferential" indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are used only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this invention.

[0028] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one of that feature. In the description of this invention, "a plurality of" means at least two, such as two, three, etc., unless otherwise explicitly specified.

[0029] In this invention, unless otherwise explicitly specified and limited, the terms "installation," "connection," "linking," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components, unless otherwise explicitly limited. Those skilled in the art can understand the specific meaning of the above terms in this invention according to the specific circumstances.

[0030] In this invention, unless otherwise explicitly specified and limited, "above" or "below" the second feature can mean that the first feature is in direct contact with the second feature, or that the first feature is in indirect contact with the second feature through an intermediate medium. Furthermore, "above," "over," and "on top" of the second feature can mean that the first feature is directly above or diagonally above the second feature, or simply that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature can mean that the first feature is directly below or diagonally below the second feature, or simply that the first feature is at a lower horizontal level than the second feature.

[0031] It should be noted that when an element is referred to as being "fixed to" or "set on" another element, it can be directly on the other element or there may be an intervening element. When an element is considered to be "connected to" another element, it can be directly connected to the other element or there may be an intervening element. The terms "vertical," "horizontal," "upper," "lower," "left," "right," and similar expressions used herein are for illustrative purposes only and do not represent the only possible implementation.

[0032] In the description of this invention, "several" means one or more, "multiple" means two or more, "greater than," "less than," and "exceeding" are understood to exclude the stated number, while "above," "below," and "within" are understood to include the stated number. The use of "first" and "second" in the description is merely for distinguishing technical features and should not be construed as indicating or implying relative importance, or implicitly indicating the number of indicated technical features, or implicitly indicating the order of the indicated technical features.

[0033] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. The terminology used herein in the specification of this invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.

[0034] This application provides an immunomicrofluidic chip 10 to address at least one of the following problems in conventional technologies: manual sample addition is cumbersome and may lead to unavoidable reagent contamination, resulting in experimental errors; it cannot achieve in-situ sample addition and cannot utilize more advanced direct chemiluminescence; and instrument reagent needles require complex liquid circuit systems for sample addition and cleaning, generating waste liquid and causing contamination from the reagent needles. The immunomicrofluidic chip 10 will be described below with reference to the accompanying drawings.

[0035] The immune microfluidic chip 10 provided in this application embodiment is exemplary; please refer to [link to example]. Figure 1 As shown, Figure 1 This is a schematic diagram of the structure of the immunomicrofluidic chip 10 provided in an embodiment of this application. The immunomicrofluidic chip 10 of this application can be used for sample pretreatment, mixing, reaction, separation and detection, and can be applied in fields such as chemical analysis, DNA sequencing, protein analysis, single-cell analysis, single-molecule analysis, food safety, environmental monitoring and drug screening.

[0036] To more clearly illustrate the structure of the immune microfluidic chip 10, the following description of the immune microfluidic chip 10 will be provided in conjunction with the accompanying drawings.

[0037] For example, please refer to Figure 1 As shown, Figure 1 This is a schematic diagram of the structure of the immune microfluidic chip 10 provided in the embodiments of this application.

[0038] An immune microfluidic chip 10 includes a microfluidic chip body 100, a puncture component 200, and a liquid sac component 300. The microfluidic chip body 100 is provided with a puncture chamber 101 and a reaction chamber 102. The puncture chamber 101 is connected to the reaction chamber 102 through a connecting channel 103. The puncture component 200 is disposed in the puncture chamber 101, and the liquid sac component 300 is connected to the puncture chamber 101. The liquid sac component 300 is elastic. When the liquid sac component 300 is pressed until it contacts the puncture component 200, the puncture component 200 can puncture the liquid sac component 300 to release the liquid inside the liquid sac component 300.

[0039] In some embodiments, the microfluidic chip body 100 is provided with a plurality of puncture chambers 101 and a plurality of reaction chambers 102. One or more puncture chambers 101 are connected to one or more reaction chambers 102 through connecting channels 103, and each puncture chamber 101 is connected to a liquid bladder component 300. The above arrangement enables multiple puncture chambers 101 to provide reaction fluid to multiple reaction chambers 102, thereby improving reaction efficiency.

[0040] In some embodiments, the number of puncture chambers 101 can be set according to actual needs, and the number of reaction chambers 102 can also be set according to actual needs. When there are multiple puncture chambers 101 and multiple reaction chambers 102, the communication relationship between the puncture chambers 101 and the reaction chambers 102 can be set according to actual needs. It is possible to achieve communication between one puncture chamber 101 and multiple reaction chambers 102, or multiple puncture chambers 101 and one reaction chamber 102, or even multiple puncture chambers 101 and multiple reaction chambers 102.

[0041] In some embodiments, the outer periphery of the fluid bladder component 300 is sealed to the inner wall of the puncture cavity 101.

[0042] In some embodiments, the liquid bladder component 300 is a plastic liquid bladder, an aluminum foil liquid bladder, or an aluminum composite liquid bladder. That is, the liquid bladder component 300 is made of one of the following materials: plastic, aluminum foil, or aluminum-plastic composite material.

[0043] In some embodiments, the fluid bladder component 300 is fixed to the puncture cavity 101 by means of bonding, laser processing, heat sealing or ultrasonic welding.

[0044] In some embodiments, the puncture component 200 is a columnar structure. The columnar structure facilitates the user's puncture of the fluid-filled bladder component 300. During use, the user only needs to gently press the fluid-filled bladder component 300 towards the puncture cavity 101. Once the columnar structure contacts the fluid-filled bladder component 300, the fluid-filled bladder component 300 can be punctured, releasing the fluid inside the fluid-filled bladder component 300.

[0045] In some embodiments, the end of the puncture member 200 facing the fluid bladder member 300 has a pointed structure.

[0046] In some embodiments, the puncture component 200 is one or more of the following shapes: needle-shaped, serrated, and cross-shaped. For example, in one specific instance, the puncture component 200 is needle-shaped; in another specific instance, the puncture component 200 is serrated; and in yet another specific instance, the puncture component 200 is cross-shaped.

[0047] In some embodiments, the reaction chamber 102 contains magnetic beads and microspheres to achieve solid-phase deposition of antigens and antibodies, thereby separating immune complexes.

[0048] In some embodiments, the reaction chamber 102 has a microporous structure to achieve the solidification of antigens and antibodies and the separation of immune complexes.

[0049] An embodiment of this application also provides the application of the immune microfluidic chip 10.

[0050] The aforementioned immunomicrofluidic chip 10 is used in chemical analysis, DNA sequencing, protein analysis, single-cell analysis, single-molecule analysis, food safety, environmental monitoring, and drug screening.

[0051] Example 1

[0052] This embodiment provides an immune microfluidic chip 10.

[0053] The immunomicrofluidic chip 10 of this embodiment includes a microfluidic chip body 100, a puncture component 200, and a liquid sac component 300. The microfluidic chip body 100 is provided with a puncture chamber 101 and a reaction chamber 102. The puncture chamber 101 is connected to the reaction chamber 102 through a connecting channel 103. The puncture component 200 is provided in the puncture chamber 101. The liquid sac component 300 is connected to the puncture chamber 101. The liquid sac component 300 is elastic. When the liquid sac component 300 is pressed until it contacts the puncture component 200, the puncture component 200 can puncture the liquid sac component 300 to release the liquid in the liquid sac component 300.

[0054] The liquid-filled balloon component 300 is made of plastic. The liquid-filled balloon component 300 is a plastic liquid-filled balloon. The liquid-filled balloon component 300 is fixed to the puncture cavity 101 by adhesive bonding. The outer periphery of the liquid-filled balloon component 300 is sealed to the inner wall of the puncture cavity 101.

[0055] The puncture component 200 has a columnar structure. The end of the puncture component 200 facing the fluid sac component 300 has a pointed structure. The puncture component 200 is needle-shaped.

[0056] The reaction chamber 102 contains magnetic beads and microspheres to achieve the solid-phase separation of antigens and antibodies, thus enabling the separation of immune complexes.

[0057] Example 2

[0058] This embodiment provides a microfluidic chip 10 based on direct chemiluminescence of acridine esters.

[0059] The microfluidic chip 10 based on acrid ester direct chemiluminescence in this embodiment includes a microfluidic chip body 100, a puncture component 200, and a liquid sac component 300. The microfluidic chip body 100 is provided with a puncture cavity 101 and a reaction cavity 102. The puncture cavity 101 is connected to the reaction cavity 102 through a connecting channel 103. The puncture component 200 is provided in the puncture cavity 101, and the liquid sac component 300 is connected to the puncture cavity 101. The liquid sac component 300 is elastic. When the liquid sac component 300 is pressed until it contacts the puncture component 200, the puncture component 200 can puncture the liquid sac component 300 to release the liquid inside the liquid sac component 300.

[0060] The liquid bladder component 300 is made of aluminum foil. The liquid bladder component 300 is an aluminum foil liquid bladder. The liquid bladder component 300 is fixed to the puncture cavity 101 by laser processing. The outer periphery of the liquid bladder component 300 is sealed to the inner wall of the puncture cavity 101.

[0061] The puncture component 200 has a columnar structure. The end of the puncture component 200 facing the fluid sac component 300 has a pointed structure and is serrated.

[0062] The reaction chamber 102 has a microporous structure to achieve the solidification of antigens and antibodies and the separation of immune complexes.

[0063] Example 3

[0064] This embodiment provides a microfluidic chip 10 based on direct chemiluminescence of acridine esters.

[0065] The microfluidic chip 10 based on acrid ester direct chemiluminescence in this embodiment includes a microfluidic chip body 100, a puncture component 200, and a liquid sac component 300. The microfluidic chip body 100 is provided with a puncture cavity 101 and a reaction cavity 102. The puncture cavity 101 is connected to the reaction cavity 102 through a connecting channel 103. The puncture component 200 is provided in the puncture cavity 101, and the liquid sac component 300 is connected to the puncture cavity 101. The liquid sac component 300 is elastic. When the liquid sac component 300 is pressed until it contacts the puncture component 200, the puncture component 200 can puncture the liquid sac component 300 to release the liquid inside the liquid sac component 300.

[0066] The microfluidic chip body 100 is provided with a number of puncture chambers 101 and a number of reaction chambers 102. One or more puncture chambers 101 are connected to one or more reaction chambers 102 through a connecting channel 103. Each puncture chamber 101 is connected to a liquid bladder component 300.

[0067] The liquid bladder component 300 is made of aluminum-plastic composite material. The liquid bladder component 300 is an aluminum composite liquid bladder. The liquid bladder component 300 is fixed to the puncture cavity 101 by ultrasonic welding. The outer periphery of the liquid bladder component 300 is sealed to the inner wall of the puncture cavity 101.

[0068] The puncture component 200 has a columnar structure. The end of the puncture component 200 facing the fluid sac component 300 has a pointed structure. The puncture component 200 is cross-shaped.

[0069] The reaction chamber 102 has a microporous structure to achieve the solidification of antigens and antibodies and the separation of immune complexes.

[0070] Example 4

[0071] This embodiment provides a microfluidic chip 10 based on direct chemiluminescence of acridine esters.

[0072] The microfluidic chip 10 based on acrid ester direct chemiluminescence in this embodiment includes a microfluidic chip body 100, a puncture component 200, and a liquid sac component 300. The microfluidic chip body 100 is provided with a puncture cavity 101 and a reaction cavity 102. The puncture cavity 101 is connected to the reaction cavity 102 through a connecting channel 103. The puncture component 200 is provided in the puncture cavity 101, and the liquid sac component 300 is connected to the puncture cavity 101. The liquid sac component 300 is elastic. When the liquid sac component 300 is pressed until it contacts the puncture component 200, the puncture component 200 can puncture the liquid sac component 300 to release the liquid inside the liquid sac component 300.

[0073] The microfluidic chip body 100 is provided with a puncture chamber 101 and several reaction chambers 102. The multiple puncture chambers 101 are connected to a reaction chamber 102 through a connecting channel 103. Each puncture chamber 101 is connected to a liquid bladder component 300.

[0074] The liquid bladder component 300 is made of aluminum-plastic composite material. The liquid bladder component 300 is an aluminum composite liquid bladder. The liquid bladder component 300 is fixed to the puncture cavity 101 by ultrasonic welding. The outer periphery of the liquid bladder component 300 is sealed to the inner wall of the puncture cavity 101.

[0075] The puncture component 200 has a columnar structure. The end of the puncture component 200 facing the fluid sac component 300 has a pointed structure. The puncture component 200 is cross-shaped.

[0076] The reaction chamber 102 contains magnetic beads and microspheres to achieve the solid-phase separation of antigens and antibodies, thus enabling the separation of immune complexes.

[0077] The reaction chamber 102 has a microporous structure to achieve the solidification of antigens and antibodies and the separation of immune complexes.

[0078] Example 5

[0079] This embodiment provides a microfluidic chip 10 based on direct chemiluminescence of acridine esters.

[0080] The microfluidic chip 10 based on acrid ester direct chemiluminescence in this embodiment includes a microfluidic chip body 100, a puncture component 200, and a liquid sac component 300. The microfluidic chip body 100 is provided with a puncture cavity 101 and a reaction cavity 102. The puncture cavity 101 is connected to the reaction cavity 102 through a connecting channel 103. The puncture component 200 is provided in the puncture cavity 101, and the liquid sac component 300 is connected to the puncture cavity 101. The liquid sac component 300 is elastic. When the liquid sac component 300 is pressed until it contacts the puncture component 200, the puncture component 200 can puncture the liquid sac component 300 to release the liquid inside the liquid sac component 300.

[0081] The microfluidic chip body 100 is provided with a puncture chamber 101 and several reaction chambers 102. The multiple puncture chambers 101 are connected to a reaction chamber 102 through a connecting channel 103. Each puncture chamber 101 is connected to a liquid bladder component 300.

[0082] The liquid bladder component 300 is made of aluminum-plastic composite material. The liquid bladder component 300 is an aluminum composite liquid bladder. The liquid bladder component 300 is fixed to the puncture cavity 101 by ultrasonic welding. The outer periphery of the liquid bladder component 300 is sealed to the inner wall of the puncture cavity 101.

[0083] The puncture component 200 has a columnar structure. The end of the puncture component 200 facing the fluid sac component 300 has a pointed structure. There are two puncture components 200, one of which is cross-shaped and the other is needle-shaped.

[0084] The reaction chamber 102 has a microporous structure to achieve the solidification of antigens and antibodies and the separation of immune complexes.

[0085] In summary, the immunomicrofluidic chip 10 of the present invention integrates a bag for storing liquid onto the microfluidic chip and releases the liquid using external force, avoiding the manual sample addition procedure, simplifying the operation steps, reducing reagent contamination, and reducing experimental errors caused by manual operation. It is expected to be widely used in microfluidic chip systems that use external force as the driving force.

[0086] In the above embodiments, the descriptions of each embodiment have different focuses. For parts not described in detail in a certain embodiment, please refer to the relevant descriptions in other embodiments.

[0087] The technical features of the above embodiments can be combined in any way. For the sake of brevity, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0088] The embodiments described above are merely illustrative of several implementations of the present invention, and while the descriptions are specific and detailed, they should not be construed as limiting the scope of the present invention. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of the present invention, and these modifications and improvements all fall within the scope of protection of the present invention. Therefore, the scope of protection of this patent should be determined by the appended claims.

Claims

1. An immune microfluidic chip, characterized in that, The device includes a microfluidic chip body, a puncture component, and a liquid-filled bladder component. The microfluidic chip body has a puncture chamber and a reaction chamber. The puncture chamber is connected to the reaction chamber via a connecting channel. The reaction chamber has a microporous structure to achieve the solid-phase deposition of antigens and antibodies, thereby separating immune complexes. The puncture component is disposed within the puncture chamber, and the liquid-filled bladder component is connected to the puncture chamber. The liquid-filled bladder component is elastic, and its outer periphery is sealed to the inner wall of the puncture chamber. The liquid-filled bladder component is fixed to the puncture chamber by means of bonding, laser treatment, heat sealing, or ultrasonic welding. When the liquid-filled bladder component is pressed until it contacts the puncture component, the puncture component can puncture the liquid-filled bladder component to release the liquid inside the liquid-filled bladder component.

2. The immune microfluidic chip according to claim 1, characterized in that, The microfluidic chip body is provided with a number of puncture chambers and a number of reaction chambers. One or more of the puncture chambers are connected to one or more of the reaction chambers through connecting channels, and each of the puncture chambers is connected to the liquid bladder component.

3. The immune microfluidic chip according to claim 1, characterized in that, The liquid bladder component is a plastic liquid bladder, an aluminum foil liquid bladder, or an aluminum composite liquid bladder.

4. The immune microfluidic chip according to claim 1, characterized in that, The puncture component has a columnar structure.

5. The immune microfluidic chip according to claim 4, characterized in that, The end of the puncture component facing the fluid sac component has a pointed structure.

6. The immune microfluidic chip according to any one of claims 1-5, characterized in that, The puncture component is one or more of the following shapes: needle-shaped, serrated, and cross-shaped.

7. The immune microfluidic chip according to any one of claims 1-5, characterized in that, The reaction chamber contains magnetic beads and microspheres to achieve the solid-phase deposition of antigens and antibodies, thereby enabling the separation of immune complexes.

8. The application of the immunomicrofluidic chip according to any one of claims 1-7 in chemical analysis, DNA sequencing, protein analysis, single-cell analysis, single-molecule analysis, food safety, environmental monitoring, or drug screening.

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