A highly airtight streaked culture dish loading box
By designing a high-closed scribing petri dish loading box, using snap connections and magnetic scribing heads, combined with elastic baffles and channel structures, the problems of sample contamination and cumbersome operation during microbial culture are solved, and efficient and safe contactless scribing operation is achieved.
Patent Information
- Application Number
- CN202310258143.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2023-03-14
- Publication Date
- 2025-08-29
- Estimated Expiration
- 2043-03-14
AI Technical Summary
In the prior art, the culture medium and samples are easily contaminated during the microbial culture process, and the operation is cumbersome, which is not suitable for batch automation, and the marking process lacks effective internal and external environmental isolation.
A high-enclosed scribing petri dish loading box is designed, including a base, a lid and functional parts, using snap connections and magnetic scribing heads, combining elastic baffles and channel structures to realize non-contact scribing and provide a fully enclosed operating environment.
It improves the efficiency and safety of the marking process, prevents sample and culture medium contamination, simplifies the operation process, is suitable for use in automated equipment, and improves production efficiency.
Smart Images

Figure CN116280651B_ABST
Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of microbial culture equipment, and in particular relates to a highly airtight lined culture dish loading box. Background Art
[0002] In activities such as microbial culture testing, it is necessary to use culture media to culture microorganisms and proliferate them for testing and research. The culture medium is placed in a culture dish, and the biological sample is dipped into an inoculation loop and then a line path is drawn on the surface of the culture medium to distribute the sample to the surface of the culture medium. In most cases, the biological sample needs to be pretreated with a specific agent, then formed into a dispersed suspension, and finally streaked. Since the standard culture dish is only a cylindrical box and does not have the function of directly completing the above-mentioned multiple procedures, it is necessary to cooperate with multiple sample containers and preparation tools to transfer, prepare, and streak materials. This process causes the culture medium and samples to be exposed to the environment multiple times, increasing the probability of contamination of the sample and culture medium. At the same time, the cumbersome operation is not conducive to the realization of batch automated culture. Summary of the Invention
[0003] The purpose of the present invention is to provide a highly sealed marking culture dish loading box suitable for non-contact marking operations, which can effectively ensure the isolation effect of the internal and external environments during the marking process, improve the efficiency and safety of the marking process, and ensure the effectiveness of the culture process.
[0004] To achieve the above objectives, the present invention adopts the following technical solutions.
[0005] A highly airtight streaked culture dish loading box, comprising a base 1, a culture dish 2, a box cover 3, and a functional part 4;
[0006] A lower receiving groove 1a is provided on the top of the base 1, and the shape of the lower receiving groove 1a is consistent with the shape of the culture dish 2;
[0007] The front side of the base 1 and the rear side of the functional component 4 are provided with mutually cooperating snap-fit connection structures, and the functional component 4 is fixed to the front side of the base 1 through the snap-fit connection structure;
[0008] The box cover 3 is buckled on the top of the base 1 and the functional part 4. The bottom of the box cover 3 is provided with an upper receiving groove 3a. The shape of the upper receiving groove 3a is consistent with the shape of the culture dish 2. The front side of the upper receiving groove 3a is provided with an inner entrance 3b;
[0009] Elastic blocking pieces 6 are provided on both sides of the inner inlet and outlet 3b; the outer sides of the two elastic blocking pieces 6 are connected to the box cover 3, and the inner sides are close to the center of the inner inlet and outlet 3b and cover the inner inlet and outlet 3b; the lower receiving groove 1a and the upper receiving groove 3a are arranged opposite each other and enclose a culture dish receiving chamber, and the culture dish 2 is arranged in the culture dish receiving chamber;
[0010] The functional part 4 is provided with a collection hole 4a, and a magnetic marking head 5 is provided in the collection hole 4a;
[0011] An external inlet and outlet 4b communicating with the collection hole 4a is provided on the front side of the functional component 4, and the external inlet and outlet 4b is directly connected with the internal inlet and outlet 3b.
[0012] As a further improvement or preferred embodiment of the aforementioned highly sealed lined culture dish loading box, a filling hole 4c is further provided on the functional part 4, and a channel connected to the collection hole 4a is provided at the bottom of the filling hole 4c; a supplementary hole 3c connected to the filling hole 4c is provided on the box cover 3.
[0013] A further improvement or preferred embodiment of the aforementioned high-sealing lined culture dish loading box is that the bottom of the channel connecting the filling hole 4c and the collection hole 4a is a slope, and the higher side of the slope is located at the bottom of the filling hole 4c, and the lower side is located at the bottom of the collection hole 4a.
[0014] In a further improvement or preferred embodiment of the aforementioned highly airtight streaked culture dish loading box, the functional component 4 is composed of an upper shell 40 and a lower shell 41 connected by snap fasteners;
[0015] The top of the lower shell 41 is open, and transverse clips 410 for plugging with the base 1 are provided on both sides of the lower shell 41. The channel connecting the filling hole 4c and the collection hole 4a is surrounded by the inner wall of the lower shell 41;
[0016] The communicating groove 401 and the filling hole 4c are formed by the local downward depression of the upper shell 40, and the collecting hole 4a is formed by the downward depression of the bottom structure of the communicating groove 401; the bottoms of the collecting hole 4a and the filling hole 4c are connected with the inner cavity of the lower shell 41, and the external inlet and outlet 4b are dug out from the rear side wall of the communicating groove 401.
[0017] As a further improvement or preferred embodiment of the aforementioned highly sealed lined culture dish loading box, vertical clips 402 are provided on both sides of the lower end surface of the upper shell 40 , and corresponding hole groove structures that can be snapped with the vertical clips 402 are provided on the lower shell 41 .
[0018] In a further improvement or preferred embodiment of the aforementioned highly airtight streaked culture dish loading box, the elastic blocking piece 6 is made of PVC material and fixed to both sides of the inner inlet and outlet 3b by adhesive.
[0019] Its beneficial effects are:
[0020] The highly sealed streaking culture dish loading box of the present application can be used to construct a fixing, supporting and protective isolation structure for traditional culture dishes, so as to facilitate the use of various types of automated streaking equipment. Without the need to change the structure of the standard culture dish equipment, the fixing effect of the culture dish during the streaking process is improved, and at the same time, a structural basis is provided for streaking operations in a non-contact fully enclosed environment. In combination with an external device equipped with a magnetic operating rod, a fully enclosed streaking operation can be achieved. Compared with the existing structure, the loading box structure is more compact, more convenient to use, and the sample filling and loading process is more efficient. At the same time, the entry and exit method of the streaking head is simpler, and the entry and exit resistance is smaller, which is conducive to further improving the production efficiency of the automatic streaking equipment and preventing problems such as the streaking head falling due to insufficient magnetic force due to excessive resistance. BRIEF DESCRIPTION OF THE DRAWINGS
[0021] Figure 1 This is a front view of a highly sealed streaked culture dish loading box;
[0022] Figure 2 It is the assembly of the high-sealed streaked culture dish loading box Figure 1 ;
[0023] Figure 3 It is the assembly of the high-sealed streaked culture dish loading box Figure 2 ;
[0024] Figure 4 This is a front view of the high-sealing streaked culture dish loading box (open);
[0025] Figure 5 is an oblique view of a high-sealing streaked culture dish loading box (open);
[0026] Figure 6 It is a schematic diagram of the internal structure of the functional parts;
[0027] Figure 7 1 is a structural diagram of the upper shell;
[0028] Figure 8 1 is a schematic structural diagram of the lower shell;
[0029] The reference numerals include:
[0030] Base 1, lower accommodating slot 1a, culture dish 2, box cover 3, upper accommodating slot 3a, internal inlet and outlet 3b, replenishing hole 3c, functional part 4, collection hole 4a, external inlet and outlet 4b, filling hole 4c, upper shell 40, connecting slot 401, vertical card strip 402, lower shell 41, horizontal card strip 410, magnetic marking head 5, elastic baffle 6. DETAILED DESCRIPTION
[0031] The present invention is described in detail below with reference to specific embodiments.
[0032] The present application discloses a highly sealed streaking culture dish loading box, which is mainly used to fix and support traditional culture dishes, improve the sealing performance of the inside and outside of the culture dishes during the streaking process, and prevent the sample and culture medium from being contaminated.
[0033] Its main structure is as follows Figures 1 to 5 As shown, it includes a base 1 for supporting and positioning a culture dish 2, a culture dish for holding culture medium, a box cover 3 for protection and isolation, and a functional part 4 for facilitating streaking and sample dispersion; to ensure the safety of biological materials, the base 1, the box cover 3 and the functional part 4 should be processed and manufactured using materials and processes that meet the requirements of the corresponding health and technical specifications.
[0034] In actual use, the base 1 needs to cooperate with various automatic or manual marking equipment to support and position the culture dish and prevent it from moving during the marking process. Therefore, a lower receiving groove 1a is provided on the top of the base 1. The shape of the lower receiving groove 1a is consistent with the shape of the culture dish 2;
[0035] When in use, the culture dish is placed in the lower receiving groove 1a. In actual production, the base 1 preferably adopts a plastic shell structure and a double-layer shell. The inner shell surrounds the lower receiving groove 1a, and the outer shell can be provided with a support or connection structure with the marking equipment and other structures, while isolating and protecting the inner shell.
[0036] In order to facilitate connection and fixation, mutually cooperating snap-fit connection structures are provided on the front side of the base 1 and the rear side of the functional component 4. The functional component 4 is fixed to the front side of the base 1 through the snap-fit connection structure.
[0037] The bayonet connection method is simple and efficient, and can realize the rapid assembly of the base 1 and the functional part 4. In actual use, the commonly used bayonet connection method can be selected according to the front and back docking method of the two.
[0038] The cover 3 is mainly used for protection and isolation and as part of the marking control structure. The cover 3 is buckled on the top of the base 1 and the functional component 4. The bottom of the cover 3 is provided with an upper receiving groove 3a. The shape of the upper receiving groove 3a is consistent with the shape of the culture dish 2. The front side of the upper receiving groove 3a is provided with an inner inlet and outlet 3b; elastic blocking pieces 6 are respectively provided on both sides of the inner inlet and outlet 3b.
[0039] The outer sides of the two elastic blocking pieces 6 are connected to the box cover 3, and the inner sides are close to the center of the inner inlet and outlet 3b and cover the inner inlet and outlet 3b; the lower receiving groove 1a and the upper receiving groove 3a are arranged opposite each other and enclose a culture dish receiving cavity, and the culture dish 2 is arranged in the culture dish receiving cavity;
[0040] The functional part 4 is provided with a collection hole 4a, in which a magnetic marking head 5 is provided. The front side of the functional part 4 is provided with an external inlet and outlet 4b communicating with the collection hole 4a, and the external inlet and outlet 4b is directly connected with the internal inlet and outlet 3b.
[0041] In actual use, the marking head located in the collection hole 4a can be controlled to move by magnetic attraction, and enter the culture dish after passing through the external inlet and outlet 4b and the internal inlet and outlet 3b to perform the marking operation. During the operation of the magnetic attraction structure, there is no need to directly open the box cover.
[0042] Two elastic baffles form the isolation structure between the internal space of the culture dish and the external marking head access channel. The elastic baffle structure is simple and does not require complex assembly methods. In actual implementation, the elastic baffle 6 can be made of PVC material and fixed to both sides of the internal inlet and outlet 3b by adhesive. When the marking head is driven by magnetic force, the elastic baffle will be compressed and deformed to open, allowing the marking head to enter and exit smoothly. After the marking head leaves, the elastic baffle returns to its original shape, thereby achieving automatic protection and isolation. The use of elastic baffle 6 can effectively maintain the isolation effect between the culture dish and the external environment. The elastic baffle 6 has a simple structure and is easy to operate and use, effectively ensuring the smoothness of the marking process.
[0043] The collection hole 4a is used to make the marking head contact with the sample or process the sample. In order to better achieve the sealing effect and facilitate the replenishment of samples or reagents, a filling hole 4c is also provided on the functional part 4. The bottom of the filling hole 4c is provided with a channel connected to the collection hole 4a; samples or reagents can be added through the filling hole and enter the collection hole through the channel, so that the marking head inside the collection hole can contact and carry the sample. In this way, there is no need to open or disassemble the functional part for operation, and the channel is used to achieve relative closed isolation of the collection hole. A replenishment hole 3c connected to the filling hole 4c is provided on the box cover 3 for easy operation.
[0044] In order to improve the efficiency of samples and reagents reaching the collection hole and prevent them from staying in the filling hole after filling, as a preferred solution, the bottom of the channel connecting the filling hole 4c and the collection hole 4a is a slope, and the higher side of the slope is located at the bottom of the filling hole 4c, and the lower side is located at the bottom of the collection hole 4a, so that the sample or reagent can automatically flow into the collection hole by utilizing the fluid's own weight.
[0045] The functional part 4 can be manufactured as a whole or adopt other structures as a supporting structure of the culture dish. It should be as light as possible and easy to assemble and disassemble. Therefore, as a preferred solution, in this embodiment, the functional part 4 is composed of an upper shell 40 and a lower shell 41 connected by a snap fastener;
[0046] The top of the lower shell 41 is open, and transverse clips 410 for plugging with the base 1 are provided on both sides of the lower shell 41. The channel connecting the filling hole 4c and the collection hole 4a is surrounded by the inner wall of the lower shell 41;
[0047] The communicating groove 401 and the filling hole 4c are formed by the local downward depression of the upper shell 40, and the collecting hole 4a is formed by the downward depression of the bottom structure of the communicating groove 401; the bottoms of the collecting hole 4a and the filling hole 4c are connected with the inner cavity of the lower shell 41, and the external inlet and outlet 4b are dug out from the rear side wall of the communicating groove 401.
[0048] The panel-shell splicing structure is simpler and more compact, allowing for easy design and fabrication of internal channels and a lighter structure. The panel-shell adapts to the plug-in structure, making assembly and disassembly easy. When necessary, the internal channel status and information can be directly observed, facilitating analysis and testing during the culture process. To facilitate connection, vertical clips 402 are provided on both sides of the lower end surface of the upper shell 40. Corresponding slots are provided on the lower shell 41 to engage with the vertical clips 402. The clips and slots provide a flexible connection between the two. Once connected, the upper shell is held down by the lid, further ensuring overall structural stability.
[0049] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the essence and scope of the technical solutions of the present invention.
Claims
1. A highly airtight streaked culture dish loading box, characterized in that: It includes a base (1), a culture dish (2), a box cover (3), and a functional part (4); A lower receiving groove (1a) is provided on the top of the base (1), and the shape of the lower receiving groove (1a) is consistent with that of the culture dish (2); The front side of the base (1) and the rear side of the functional component (4) are provided with mutually cooperating snap-fit connection structures, and the functional component (4) is fixed to the front side of the base (1) via the snap-fit connection structure; The box cover (3) is buckled on the top of the base (1) and the functional component (4); the bottom of the box cover (3) is provided with an upper receiving groove (3a); the shape of the upper receiving groove (3a) is consistent with the shape of the culture dish (2); the front side of the upper receiving groove (3a) is provided with an inner inlet and outlet (3b); Elastic blocking pieces (6) are respectively provided on both sides of the inner inlet and outlet (3b); the outer sides of the two elastic blocking pieces (6) are connected to the box cover (3), and the inner sides are close to the center of the inner inlet and outlet (3b) and block the inner inlet and outlet (3b); the lower accommodating groove (1a) and the upper accommodating groove (3a) are arranged opposite to each other and enclose a culture dish accommodating cavity, and the culture dish (2) is arranged in the culture dish accommodating cavity; The functional part (4) is provided with a collection hole (4a), and a magnetic marking head (5) is provided in the collection hole (4a); an external inlet and outlet (4b) communicating with the collection hole (4a) is provided on the front side of the functional part (4), and the external inlet and outlet (4b) is directly connected to the internal inlet and outlet (3b).
2. The highly sealed streaked culture dish loading box according to claim 1, characterized in that: The functional part (4) is also provided with a filling hole (4c), and a channel connected to the collection hole (4a) is provided at the bottom of the filling hole (4c); and the box cover (3) is provided with a supplementary hole (3c) connected to the filling hole (4c).
3. The highly sealed streaked culture dish loading box according to claim 2, characterized in that: The bottom of the channel connecting the filling hole (4c) and the collection hole (4a) is an inclined surface, with the higher side of the inclined surface located at the bottom of the filling hole (4c) and the lower side located at the bottom of the collection hole (4a).
4. The highly sealed streaked culture dish loading box according to claim 3, characterized in that: The functional part (4) is composed of an upper shell (40) and a lower shell (41) connected by a snap fastener; The top of the lower shell (41) is open, and transverse clamping strips (410) for plugging with the base (1) are provided on both sides of the lower shell (41), and a channel connecting the filling hole (4c) and the collection hole (4a) is surrounded by the inner wall of the lower shell (41); The upper shell (40) is partially recessed downward to form a connecting groove (401) and a filling hole (4c), and the collection hole (4a) is formed by a downward recess of the bottom structure of the connecting groove (401). The bottoms of the collection hole (4a) and the filling hole (4c) are in communication with the inner cavity of the lower shell (41), and the external inlet and outlet (4b) are dug out from the rear side wall of the connecting groove (401).
5. The highly sealed streaked culture dish loading box according to claim 4, characterized in that: Vertical clamping strips (402) are provided on both sides of the lower end surface of the upper shell (40), and corresponding hole groove structures capable of being clamped with the vertical clamping strips (402) are provided on the lower shell (41).
6. The highly sealed streaked culture dish loading box according to claim 1, characterized in that: The elastic blocking piece (6) is made of PVC material and is fixed to both sides of the inner inlet and outlet (3b) by adhesive.
Citation Information
Patent Citations
Anti-pollution streak culture box
CN113881547A
Culture dish positioning assembly of automatic streak culture equipment
CN114525199A