Oral and nasal inhalation self-administration device and method of use

Abstract

The present invention discloses an oral and nasal inhalation self-medication device and a method for use. The device comprises an aerosol nebulizer containing liquid medicine, an aerosol buffer chamber, a controller, multiple chambers, and multiple drug delivery devices. The chamber comprises multiple nose contact devices, which include a nose contact block, a photoelectric sensor, an air extraction channel, and an air intake channel. The nose contact block is provided with a hollow cavity, and the air extraction channel and the air intake channel are both connected to the hollow cavity. The photoelectric sensor includes a receiving end and a transmitting end. The drug delivery device comprises an air extraction pump, a three-way valve, and an exhaust gas filter. When an animal's nose is inserted into one of the hollow cavities, aerosolized medicine is provided to the animal. After the set aerosolized medicine supply time is completed, the aerosolized medicine enters the exhaust gas filter through a third interface. The oral and nasal inhalation self-medication device and method for use provided by the present invention better simulate the oral and nasal inhalation of drugs by animals, thereby improving the accuracy and experimental efficiency of the results of animal drug addiction experiments.

Description

Technical Field

[0001] The present invention relates to the technical field of animal addiction behavior, and more specifically, to an oral and nasal inhalation self-medication device and a method of use. Background Art

[0002] Self-administration experiments are based on the principle of operant conditioning. After completing a pre-set action program, experimental animals are given a certain amount of medication, which acts as a reward and reinforces their behavior. The self-administration model is a classic animal model that reflects the user's active drug-seeking and drug-taking behavior. It can be used to investigate drug-taking motivation and compulsive drug-taking behavior. Self-administration can be achieved through almost all clinical routes of administration, with intravenous administration being the most commonly used, followed by intracranial, oral, and inhalation administration. Intravenous administration closely mimics the way opioids are used in humans.

[0003] However, there is still a large number of addictive substances whose common method of intake is oral intake and absorption in the respiratory tract or lungs. Intravenous self-administration lacks the process of drug absorption into the blood in the respiratory tract or lungs, and cannot well simulate the intake method of this type of substance. In addition, due to the complexity of the jugular vein catheterization surgery, the difficulty of subsequent care, and the difficulty of the overall experiment, it is very easy to cause data interruption and sample size loss during the experiment. There are currently cases of aerosol administration, but there are still many problems: First, the current aerosol self-administration is through systemic exposure, which has a certain impact on the skin, eyes, ears and other parts of the animal. The drug can also be absorbed through the skin and mucous membranes and other parts, which cannot better simulate oral and nasal inhalation, thereby reducing the accuracy of the results of animal drug addiction experiments; second, the efficiency of the experiment needs to be further improved. Summary of the Invention

[0004] In view of this, the purpose of the present invention is to propose an oral and nasal inhalation self-administration device and a method of use to better simulate the oral and nasal inhalation of drugs in animals, improve the accuracy of animal drug addiction test results, and improve experimental efficiency.

[0005] Based on the above-mentioned purpose, the present invention provides an oral and nasal inhalation type self-administration device, comprising an aerosol nebulizer containing liquid medicine, an aerosol buffer chamber, a controller, a plurality of chambers for animal activities and a plurality of drug delivery devices arranged in a one-to-one correspondence with the plurality of chambers, wherein: the chamber comprises a plurality of nose touch devices, and the plurality of nose touch devices are respectively detachably and sealedly mounted on the side walls of the chamber, the nose touch device comprises a nose contact block, a photoelectric sensor, an air extraction channel and an air intake channel, a hollow cavity is provided in the nose contact block, the air extraction channel and the air intake channel are both connected to the hollow cavity, and the air extraction channel is located above the air intake channel, the photoelectric sensor comprises a receiving end and a transmitting end, the receiving end and the transmitting end are respectively mounted on two opposite ends of the nose contact block, and infrared rays are arranged between the transmitting end and the receiving end, and the photoelectric sensor is communicatively connected to the controller; the medicine The animal conveying device includes an air suction pump, a three-way valve, and an exhaust gas filter. The air suction pump is connected to the air suction pipe. The three-way valve includes a first interface, a second interface and a third interface. The first interface is connected to the aerosol buffer chamber, the second interface is connected to the air intake pipe, and the third interface is connected to the exhaust gas filter. The three-way valve is communicatively connected to the controller; one end of the aerosol nebulizer is connected to the aerosol buffer chamber, and the other end of the aerosol nebulizer is used to be connected to compressed air; when the animal's nose is inserted into one of the hollow cavities, the animal's nose blocks the propagation path of the infrared ray. At this time, the first interface and the second interface are connected to provide aerosol medicine to the animal; when the set aerosol medicine supply time is completed, the first interface is connected to the third interface, and the aerosol medicine enters the exhaust gas filter after passing through the third interface.

[0006] Optionally, a camera is installed on the top of the chamber to record the behavior of the animal in the chamber.

[0007] Optionally, it also includes multiple sound generators, multiple warning lights and multiple cage lights corresponding to the multiple chambers one by one, the sound generators, the warning lights and the cage lights are all installed in the chambers, and the multiple warning lights are respectively installed on one side of the corresponding chambers, and the sound generators, the warning lights and the cage lights are all electrically connected to the controller.

[0008] Optionally, the chamber further includes a plurality of conductive metal rods, which are electrically connected to the controller. The plurality of conductive metal rods are laid flat above the bottom of the chamber, leaving a gap between the rods and the bottom of the chamber. A detachable collection box is provided in the gap for collecting the feces and urine of the animal.

[0009] Optionally, an optical fiber compatible slot is further provided in the nasal cavity block, and a probe is installed on the head of the animal, and the probe is used for communication connection with the optical fiber recording system.

[0010] Optionally, it further includes a plurality of sound insulation boxes arranged in one-to-one correspondence with the plurality of chambers, the sound insulation boxes are installed outside the chambers, and an environmental noise fan is installed on the sound insulation boxes.

[0011] Optionally, there are four chambers, and four nose touch devices are installed in each chamber. In each chamber, two nose touch devices are installed on the side walls of the chamber that are opposite to each other.

[0012] Optionally, the chamber is a transparent acrylic chamber.

[0013] Based on the same inventive concept, the present invention also provides a method for using an oral and nasal inhalation self-medication device, and uses the oral and nasal inhalation self-medication device described in any of the above technical solutions to conduct experiments on animals, including the following steps: Step 1, there are multiple chambers, and the air inlet channel on one of the nose touch devices in the chamber is connected and installed on the first interface, and the corresponding air exhaust channel is connected and installed on the air exhaust pump; Step 2, multiple animals are placed in the multiple chambers accordingly, and the animals are allowed to adapt for 1mi n and then start training; step three, in any of the chambers, when the animal's nose is inserted into the hollow cavity connected to the air pump, the animal's nose blocks the propagation path of the infrared rays, and at this time the first interface and the second interface are connected to provide aerosolized medicine to the animal. After the set aerosolized medicine supply time is completed, the first interface is connected to the third interface, and the aerosolized medicine directly enters the exhaust gas filter after passing through the third interface; when the animal's nose is inserted into the hollow cavity not connected to the air pump, the animal does not obtain the aerosolized medicine, and the aerosolized medicine directly enters the exhaust gas filter after passing through the first interface and the third interface; multiple animals conduct the above experiment in multiple chambers at the same time.

[0014] The present invention provides an oral and nasal inhalation self-medication device and a method of use. The oral and nasal inhalation self-medication device includes an aerosol nebulizer containing liquid medicine, an aerosol buffer chamber, a controller, multiple chambers for animal activities, and multiple drug delivery devices arranged in a one-to-one correspondence with the multiple chambers. The nose contact device includes a nose contact block, a photoelectric sensor, an air extraction channel and an air intake channel. A hollow cavity is provided in the nose contact block. First, the air intake channel on one of the nose contact devices in the chamber is connected and installed on the first interface, and the corresponding air extraction channel is connected and installed on the air extraction pump; then, multiple animals are placed in the multiple chambers accordingly. When the animal's nose is inserted into the hollow cavity connected to the air extraction pump, the animal's nose blocks the air inlet channel. The propagation path of infrared rays, at this time, the first interface and the second interface are connected to provide aerosolized medicine to the animal. When the set aerosolized medicine supply time is completed, the first interface is connected to the third interface, and the aerosolized medicine directly enters the exhaust gas filter after passing through the third interface; when the animal's nose is inserted into the hollow cavity that is not connected to the air pump, the animal does not obtain the aerosolized medicine, and the aerosolized medicine directly enters the exhaust gas filter after passing through the first interface and the third interface, which better simulates the animal's oral and nasal inhalation of the drug; multiple animals conduct the above experiment in multiple chambers at the same time, ensuring the consistency of the chamber air supply, reducing the experimental error caused by inconsistent air supply, improving the accuracy of the animal's drug addiction test results, and improving the experimental efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] The preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings, which will help to understand the objects and advantages of the present invention.

[0016] Figure 1 This is a schematic structural diagram of an oronasal inhalation self-medication device according to one embodiment of the present invention;

[0017] Figure 2 A schematic diagram of the structure and connections of a chamber in an oronasal inhalation self-medication device according to one embodiment of the present invention;

[0018] Figure 3 This is a schematic structural diagram of a nose contact device in an oronasal inhalation self-medication device according to one embodiment of the present invention;

[0019] Figure 4 A cross-sectional view of the structure of the nose contact device in the oral and nasal inhalation self-medication device according to one embodiment of the present invention;

[0020] Figure 5 A flowchart of a method for using an oronasal inhalation self-medication device according to an embodiment of the present invention;

[0021] Figure 6 This is a flow chart of the experimental procedures for using the oral and nasal inhalation self-medication device according to one embodiment of the present invention.

[0022] Description of reference numerals:

[0023] 1: Aerosol nebulizer; 2: Aerosol buffer chamber; 3: Chamber; 4: Drug delivery device; 5: Nasal contact device; 6: Nasal contact block; 7: Photoelectric sensor; 8: Exhaust channel; 9: Air inlet channel; 10: Hollow cavity; 11: Exhaust pump; 12: Three-way valve; 13: Exhaust filter; 14: Camera; 15: Cage light; 16: Warning light; 17: Conductive metal rod; 18: Fiber optic compatible slot; 19: Ambient noise fan; 20: Soundproof box. DETAILED DESCRIPTION

[0024] The present invention will be described in detail below with reference to the embodiments. Identical components are denoted by the same reference numerals. It should be noted that the terms "front," "rear," "left," "right," "upper," and "lower" used in the following description refer to directions in the accompanying drawings, and the terms "inner" and "outer" refer to directions toward or away from the geometric center of a particular component, respectively.

[0025] like Figures 1 to 4 As shown, the oral and nasal inhalation self-administration device provided by the present invention includes an aerosol nebulizer 1 containing liquid medicine, an aerosol buffer chamber 2, a controller, a plurality of chambers 3 for animal activities and a plurality of drug delivery devices 4 arranged in a one-to-one correspondence with the plurality of chambers 3, wherein: the chamber 3 includes a plurality of nose touch devices 5, and the plurality of nose touch devices 5 are respectively detachably and sealedly mounted on the side walls of the chamber 3, the nose touch devices 5 include a nose contact block 6, a photoelectric sensor 7, an air extraction channel 8 and an air intake channel 9, a hollow cavity 10 is provided in the nose contact block 6, the air extraction channel 8 and the air intake channel 9 are both connected to the hollow cavity 10, and the air extraction channel 8 is located above the air intake channel 9, the photoelectric sensor 7 includes a receiving end and a transmitting end, the receiving end and the transmitting end are respectively mounted on two opposite ends of the nose contact block 6, and infrared rays are arranged between the transmitting end and the receiving end, and the photoelectric sensor 7 is connected to the controller Communication connection; the drug delivery device 4 includes an air pump 11, a three-way valve 12, and an exhaust gas filter 13. The air pump 11 is connected to the air extraction pipeline. The three-way valve 12 includes a first interface, a second interface and a third interface. The first interface is connected to the aerosol buffer chamber 2, the second interface is connected to the air intake pipeline, and the third interface is connected to the exhaust gas filter 13. The three-way valve 12 is communicatively connected to the controller; one end of the aerosol nebulizer 1 is connected to the aerosol buffer chamber 2, and the other end of the aerosol nebulizer 1 is used to be connected to the compressed air; when the animal's nose is inserted into one of the hollow cavities 10, the animal's nose blocks the propagation path of the infrared ray. At this time, the first interface and the second interface are connected to provide aerosol medicine to the animal; when the set aerosol medicine supply time is completed, the first interface is connected to the third interface, and the aerosol medicine enters the exhaust gas filter 13 after the third interface.

[0026] The oral and nasal inhalation self-medication device provided by the present invention includes an aerosol nebulizer 1 containing liquid medicine, an aerosol buffer chamber 2, a controller, a plurality of chambers 3 for animal activities, and a plurality of drug delivery devices 4 arranged in a one-to-one correspondence with the plurality of chambers 3. The nose contact device 5 includes a nose contact block 6, a photoelectric sensor 7, an air extraction channel 8 and an air intake channel 9. A hollow cavity 10 is provided in the nose contact block 6. First, the air intake channel 9 on one of the nose contact devices 5 in the chamber 3 is connected and installed on the first interface, and the corresponding air extraction channel 8 is connected and installed on the air extraction pump 11; then, multiple animals are placed in the multiple chambers 3 accordingly. When the nose of the animal is stretched into the hollow cavity 10 connected to the air extraction pump 11, the nose of the animal blocks the red blood cell. The propagation path of the external line, at this time the first interface and the second interface are connected to provide the animal with aerosolized medicine. After the set aerosolized medicine supply time is completed, the first interface is connected to the third interface, and the aerosolized medicine directly enters the exhaust gas filter 13 after passing through the third interface; when the animal's nose is inserted into the hollow cavity 10 that is not connected to the air pump 11, the animal does not obtain the aerosolized medicine, and the aerosolized medicine directly enters the exhaust gas filter 13 after passing through the first interface and the third interface, which better simulates the animal's oral and nasal inhalation of the drug; multiple animals carry out the above-mentioned experiment in multiple chambers 3 at the same time, ensuring the consistency of the air supply in the chamber 3, reducing the experimental error caused by inconsistent air supply, improving the accuracy of the animal's drug addiction test results, and improving the experimental efficiency.

[0027] like Figure 2 As shown, a camera 14 is installed on the top of the chamber 3 to record the behavior of the animal in the chamber 3. In this embodiment, the camera 14 can record the behavior of the animal in the chamber 3 more completely, so as to better study the behavior of the animal, thereby improving the ease of use of the oral and nasal inhalation self-medication device.

[0028] like Figure 2 As shown, the device further includes multiple sound generators, multiple indicator lights 16, and multiple cage lights 15 corresponding to the multiple chambers 3. The sound generators, indicator lights 16, and cage lights 15 are all installed within the chambers 3, with the multiple indicator lights 16 being installed on one side of the corresponding chambers 3. The sound generators, indicator lights 16, and cage lights 15 are all electrically connected to a controller. In this embodiment, the cage lights 15 provide illumination for the animal's movements within the chambers 3. When the animal enters the chamber 3, the sound generator emits a sound to alert the animal to enter the chamber 3. When the animal's nose penetrates the hollow cavity 10, the indicator light 16 illuminates, making it easier to intuitively identify the animal's position within the chamber 3 and improving the ease of use of the oral and nasal inhalation self-medication device.

[0029] like Figure 2The chamber 3 shown also includes multiple conductive metal rods 17, which are electrically connected to the controller. These rods 17 are laid flat above the bottom of the chamber 3, leaving a gap between them. A removable collection box is located within this gap for collecting animal feces and urine. In this embodiment, the controller energizes the conductive metal rods 17, applying an electric current to the soles of the animal's feet. The animal's drug-seeking behavior is then observed to determine whether it is suppressed, thereby determining the animal's drug addiction. This increases the experimental diversity of oral and nasal inhalation self-administration devices.

[0030] like Figure 4 As shown, a fiber-optic compatible slot 18 is also provided within the nose contact block 6. A probe is mounted on the animal's head for communication with the fiber-optic recording system. In this embodiment, the fiber-optic compatible slot 18 prevents the probe from contacting the nose contact block 6, thereby improving the ease of use of the oral and nasal inhalation self-medication device.

[0031] like Figure 1 As shown, it also includes multiple soundproof boxes 20 arranged in a one-to-one correspondence with the multiple chambers 3. The soundproof boxes 20 are installed outside the chambers 3, and an environmental noise fan 19 is installed on the soundproof boxes 20. It should be noted that the material of the soundproof boxes 20 is wooden boards, and the inner wall of the soundproof boxes 20 is covered with rough EVA foam, thereby isolating and absorbing external noise, while soundproofing and creating a dark environment; the environmental noise fan 19 can provide mild environmental white noise and ventilation. In this embodiment, the soundproof box 20 isolates and absorbs external noise, thereby avoiding the impact of external noise on animals and improving the accuracy of the experimental results of the oral and nasal inhalation self-medication device.

[0032] like Figure 1 and Figure 2 As shown, there are four chambers 3, each of which is equipped with four nasal contact devices 5. In each chamber 3, two nasal contact devices 5 are installed on opposite side walls of the chamber 3. In this embodiment, there are two nasal contact devices 5 on the left and right sides of the chamber 3. One of the nasal contact devices 5 can be connected to the aerosolized drug, while the other three are not connected to the aerosolized drug. This increases the difficulty for the animal to obtain the drug, further observes the effect of the drug on the animal's drug-seeking behavior, and improves the ease of use of the nasal inhalation self-medication device.

[0033] In one embodiment of the present invention, the chamber 3 is a transparent acrylic chamber 3, which facilitates observation of the behavior of the animal in the chamber 3 and improves the ease of use of the nasal inhalation self-medication device.

[0034] like Figures 1 to 5As shown, based on the same inventive concept, an embodiment of the present invention further provides a method for using an oral and nasal inhalation self-medication device, and an experiment is conducted on animals using the oral and nasal inhalation self-medication device of any of the aforementioned embodiments, comprising the following steps: Step 1, there are multiple chambers 3, the air inlet channel 9 on one of the nose contact devices 5 in the chamber 3 is connected and installed on the first interface, and the corresponding air extraction channel 8 is connected and installed on the air extraction pump 11; Step 2, multiple animals are placed in the multiple chambers 3 accordingly, and the animals are allowed to adapt for 1 minute. n and then start training; step three, in any chamber 3, when the animal's nose is inserted into the hollow cavity 10 connected to the air pump 11, the animal's nose blocks the propagation path of the infrared rays, and at this time the first interface and the second interface are connected to provide aerosolized medicine to the animal. When the set aerosolized medicine supply time is completed, the first interface is connected to the third interface, and the aerosolized medicine directly enters the exhaust gas filter 13 after passing through the third interface; when the animal's nose is inserted into the hollow cavity 10 not connected to the air pump 11, the animal does not obtain the aerosolized medicine, and the aerosolized medicine directly enters the exhaust gas filter 13 after passing through the first interface and the third interface; multiple animals perform the above experiment simultaneously in multiple chambers 3.

[0035] The present invention provides an oral and nasal inhalation self-medication device and a method of use. The oral and nasal inhalation self-medication device includes an aerosol nebulizer 1 containing liquid medicine, an aerosol buffer chamber 2, a controller, multiple chambers 3 for animal activities, and multiple drug delivery devices 4 arranged in a one-to-one correspondence with the multiple chambers 3. The nose contact device 5 includes a nose contact block 6, a photoelectric sensor 7, an air extraction channel 8 and an air intake channel 9. A hollow cavity 10 is provided in the nose contact block 6. First, the air intake channel 9 on one of the nose contact devices 5 in the chamber 3 is connected and installed on the first interface, and the corresponding air extraction channel 8 is connected and installed on the air extraction pump 11; then, multiple animals are placed in the multiple chambers 3 accordingly. When the animal's nose is inserted into the hollow cavity 10 connected to the air extraction pump 11, the animal The nose of the animal blocks the propagation path of the infrared rays. At this time, the first interface and the second interface are connected to provide the animal with aerosolized medicine. After the set aerosolized medicine supply time is completed, the first interface is connected to the third interface, and the aerosolized medicine directly enters the exhaust gas filter 13 after passing through the third interface; when the nose of the animal is stretched into the hollow cavity 10 that is not connected to the air pump 11, the animal does not obtain the aerosolized medicine, and the aerosolized medicine directly enters the exhaust gas filter 13 after passing through the first interface and the third interface, which better simulates the inhalation of drugs by the animal's mouth and nose; multiple animals carry out the above experiment in multiple chambers 3 at the same time, which ensures the consistency of the air supply in the chamber 3, reduces the experimental error caused by inconsistent air supply, improves the accuracy of the results of the animal's drug addiction experiment, and improves the experimental efficiency.

[0036] Pre-experimental preparation procedures: First, fill the aerosol concentrate of the drug under study into the aerosol nebulizer 1; then, start the aerosol nebulizer 1. After the aerosol evenly fills the entire system, check the operation and connectivity of each chamber 3, simulate animal nose contact, and observe the operation of aerosol delivery, signal prompt response of the auxiliary prompt device, and waste gas recovery.

[0037] Example 1

[0038] After checking each device to ensure proper function, you can select a training program or create your own. Each chamber 3 is equipped with four nose-touch devices 5, with nose-touch device A connected to the aerosol line. When programming, select the nose-touch device and cage light 15 based on the experimental needs. In this example, nose-touch device B is set as the active nose-touch device and nose-touch device A as the inactive nose-touch device. Training is performed using a fixed frequency 1 (FR1) program. During the non-interval period, the animal receives a single touch from device B and a single aerosol delivery. Touching device A results in no response.

[0039] Experimental procedure:

[0040] First, the animal is gently placed in the animal activity room. After connecting the optical fiber of the optical fiber recording system, the doors of the chamber 3 and the soundproof box 20 are closed, and the camera 14 is started to record the video. After the animal has adapted for 1 minute, training begins.

[0041] Then, when the training program is started, the cage light 15 is always on, the sound generator makes a sound and keeps on lighting the training start prompt for 2 seconds, that is, the cage light 15 in the chamber 3 is turned on and is always on, the sound generator produces a buzzing sound for 2 seconds, the three-way valve 12 is opened, and the state enters state 1 after 3 seconds of aerosol delivery.

[0042] After the animal touches the effective nose touch, it enters state two. At this time, the cage light 15 is turned off, and the corresponding nose touch prompt light 16 lights up for 5 seconds, and the sound lasts for 1 second, which is used to strengthen the connection between lights, sounds, etc. The three-way valve 12 is opened, and the atomized medicine is sprayed out from the hollow cavity 10, and the animal inhales it in situ for 5 seconds. At the same time, the waste gas recovery pump is turned on to recycle the waste gas inhaled by the animal. After the end of state two, it enters state three, which is the interval period. During the interval period, the animal touches the nose touch again without any reaction. After the 30s interval period, it returns to state one. After the animal touches it again, it will enter state two again, and so on.

[0043] If the animal touched an invalid nose touch, the system did not respond, but the number and duration of touches were recorded.

[0044] The first phase of training ends in two situations: one is when the training program has completed the time set, and the other is when the training program has completed the number of dosing times set. Then, you can move on to the second phase of training, or end the program. The interval time and the total number of dosing times are limited to prevent animals from being injured by excessive inhalation.

[0045] The above experimental procedure can be simplified as follows Figure 6 As shown in the flowchart, during the experiment, the controller records the animal's nose-touching behavior in chamber 3, including data such as the number of effective nose-touches, the number of ineffective nose-touches, the timing of nose-touches, and the number of drug administrations. If the fluctuation in the number of aerosol administrations for three consecutive days is less than 20%, the animal has established stable aerosol self-administration behavior. During the experiment, the fiber optic recording system can be used to observe the levels of substances such as dopamine in the animal's brain in real time, and the video recordings can be combined with animal behavior analysis. After the experiment, the video can also be imported into animal behavior analysis software for simple animal behavior analysis.

[0046] After the experiment, remove the animals from chamber 3 and observe and record their condition. Clean chamber 3, remove the bottom tray, clean any feces and urine, rinse with clean water, and wipe clean with alcohol. Wipe the bottom metal connecting rod and nose contact hole with alcohol. If necessary, replace nose contact device 5 to prevent residual odor from the previous batch of animals from affecting the next batch. Observe the remaining drug solution to determine if additional solution or replacement is needed. Once clean, the next batch of animals can be trained.

[0047] Example 2

[0048] In addition to the in situ drug delivery procedure, non-in situ drug delivery can be designed. When editing the experimental program, three nasal contact holes, B, C, and D, can be selected simultaneously. B serves as the aerosol delivery channel, D is the effective nasal contact hole, and C is the ineffective nasal contact hole. When the animal touches nasal contact hole D, photoelectric sensor 7 transmits a signal to drug delivery device 4, controlling the release of aerosolized drug at contact hole B. Touching nasal contact hole C results in no reaction. Only when the animal touches the nasal contact hole at D can it successfully obtain drug at contact hole B, thereby observing the reinforcing effect of the drug.

[0049] After the experimental program is edited, the specific operation is consistent with Example 1. Considering that mice may not be able to accurately find the nose contact device 5 corresponding to the aerosolized drug in the initial stage of non-in situ drug administration, a 7-day whole-body exposure can be performed first, that is, the exhaust gas extraction pump 11 is turned off to allow the aerosolized drug to reach the animal activity chamber 3 directly. After the seven-day whole-body exposure, the mice may be in a state of addiction. Then, the exhaust pump 11 is turned on, and the exhaust pump 11 is not completely removed at first. The exhaust intensity of the exhaust pump 11 is gradually increased each day until the aerosolized drug cannot enter the animal activity chamber at all.

[0050] Example 3

[0051] In addition to studying the reinforcing effects of specific drugs after aerosol administration, this oral and nasal inhalation self-administration device can also be connected to an external smoking machine, which can be used instead of the atomizer to study the addictiveness of cigarette smoke. The specific steps are the same as those in Example 1, except that the original atomizer is replaced with an automatic smoking machine, and different cigarette products are smoked to release cigarette smoke, thereby evaluating the cigarette smoke.

[0052] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. An oral and nasal inhalation self-administration device, characterized in that: The invention comprises an aerosol nebulizer containing liquid medicine, an aerosol buffer chamber, a controller, a plurality of chambers for animal activities, and a plurality of drug delivery devices arranged in a one-to-one correspondence with the plurality of chambers, wherein: The chamber includes a plurality of nose contact devices, which are respectively detachably and sealedly mounted on the side walls of the chamber. The nose contact devices include a nose contact block, a photoelectric sensor, an air extraction channel, and an air intake channel. A hollow cavity is provided in the nose contact block, and both the air extraction channel and the air intake channel are connected to the hollow cavity, and the air extraction channel is located above the air intake channel. The photoelectric sensor includes a receiving end and a transmitting end, and the receiving end and the transmitting end are respectively mounted on opposite ends of the nose contact block, and infrared rays are provided between the transmitting end and the receiving end. The photoelectric sensor is communicatively connected to the controller. The drug delivery device includes an air pump, a three-way valve, and an exhaust gas filter. The air pump is connected to the air extraction channel. The three-way valve includes a first interface, a second interface, and a third interface. The first interface is connected to the aerosol buffer chamber, the second interface is connected to the air inlet channel, and the third interface is connected to the exhaust gas filter. The three-way valve is in communication with the controller. One end of the aerosol nebulizer is connected to the aerosol buffer chamber, and the other end of the aerosol nebulizer is used to communicate with compressed air; When the animal's nose is inserted into one of the hollow cavities, the animal's nose blocks the propagation path of the infrared rays, and the first interface and the second interface are connected to provide aerosolized medicine to the animal. After the set aerosolized medicine supply time is completed, the first interface is connected to the third interface, and the aerosolized medicine enters the exhaust filter after passing through the third interface. The system further includes a plurality of sound generators, a plurality of warning lights, and a plurality of cage lights corresponding to the plurality of chambers, wherein the sound generators, the warning lights, and the cage lights are all installed in the chambers, and the plurality of warning lights are respectively installed on one side of the corresponding chambers, and the sound generators, the warning lights, and the cage lights are all electrically connected to the controller; The chamber also includes a plurality of conductive metal rods, which are electrically connected to the controller. The plurality of conductive metal rods are laid flat above the bottom of the chamber, leaving a gap between the plurality of conductive metal rods and the bottom of the chamber. A detachable collection box is provided in the gap for collecting the feces and urine of the animal.

2. The oral and nasal inhalation self-administration device according to claim 1, characterized in that A camera is installed on the top of the chamber to record the behavior of the animal in the chamber.

3. The oral and nasal inhalation self-administration device according to claim 1, characterized in that An optical fiber compatible slot is also provided in the nose contact block, and a probe is installed on the head of the animal, and the probe is used for communication connection with the optical fiber recording system.

4. The oral and nasal inhalation self-administration device according to claim 1, characterized in that It also includes a plurality of sound insulation boxes arranged in one-to-one correspondence with the plurality of chambers. The sound insulation boxes are installed outside the chambers, and an environmental noise fan is installed on the sound insulation boxes.

5. The oral and nasal inhalation self-administration device according to claim 1, characterized in that There are four chambers, and four nose touch devices are installed in each chamber. In each chamber, two nose touch devices are installed on the side walls that are opposite to each other.

6. The oral and nasal inhalation self-administration device according to claim 1, characterized in that The chamber is a transparent acrylic chamber.

7. A method for using an oral and nasal inhalation self-administration device, characterized in that: The method of using the oral and nasal inhalation self-administration device according to any one of claims 1 to 6 to conduct an experiment on animals comprises the following steps: Step 1: There are multiple chambers, and the air inlet channel on one of the nose touch devices in the chamber is connected and installed on the first interface, and the corresponding air exhaust channel is connected and installed on the air exhaust pump; Step 2: Place multiple animals into the chambers accordingly, and start training after the animals have adapted for 1 minute; Step 3. In any of the chambers, when the animal's nose is inserted into the hollow cavity connected to the air pump, the animal's nose blocks the propagation path of the infrared rays. At this time, the first interface and the second interface are connected to provide aerosolized medicine to the animal. After the set aerosolized medicine supply time is completed, the first interface is connected to the third interface, and the aerosolized medicine directly enters the exhaust gas filter after passing through the third interface; when the animal's nose is inserted into the hollow cavity not connected to the air pump, the animal does not obtain aerosolized medicine, and the aerosolized medicine directly enters the exhaust gas filter after passing through the first interface and the third interface; multiple animals conduct the above experiment simultaneously in multiple chambers.

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