Biochip all-in-one machine

Abstract

The application provides a biochip integrated machine, which comprises a shell and a hybridization part, a reading part and a driving part arranged in the shell. The hybridization part is provided with a liquid containing assembly and a liquid suction and discharge assembly. A reactor in which a biochip is arranged is detachably arranged in the hybridization part. The liquid containing assembly is used for containing liquid. The liquid suction and discharge assembly is connected with the reactor and the liquid in the liquid containing assembly is sucked into the reactor or the liquid in the reactor is discharged by applying pressure to the reactor. The driving part transmits driving force to the reactor to drive the reactor to move between the hybridization reaction area and the reading area. The reading part is used for reading the result of the hybridized biochip in the reading area. The application can integrate the biochip hybridization and the hybridization result reading, automatically complete the biochip hybridization reaction, the result display and acquisition, and has high working efficiency, simple and convenient operation, compact structure, flexible use and long service life.

Description

Technical Field

[0001] This invention belongs to the field of biochip all-in-one machine technology, specifically a biochip all-in-one machine. Background Technology

[0002] I. Biochips and Gene Chips

[0003] Biochips (or bioarrays) are based on the principle of specific interactions between biomolecules, integrating biochemical analysis processes onto the chip surface to achieve high-throughput, rapid detection of DNA, RNA, peptides, proteins, and other biological components. In a narrower sense, a biochip refers to a matrix of biomolecules (oligonucleotides, cDNA, genomic DNA, peptides, antibodies, antigens, etc.) immobilized on solid-phase media such as silicon wafers, glass slides (beads), plastic sheets (beads), gels, and nylon membranes using various methods. Therefore, biochip technology is also known as microarray technology, and the solid-phase matrix containing a large amount of biological information is called a microarray, also known as a biochip. As part of the gene industry, biochips have a very broad application prospect. They can be applied to areas such as new gene discovery, DNA sequencing, disease diagnosis, drug screening, toxicology genomics, crop breeding and selection, environmental monitoring and control, food hygiene supervision, and forensic identification, among others.

[0004] The main characteristics of biochips are high throughput, miniaturization, and automation. The highly integrated, densely packed arrays of thousands of molecular microarrays on a biochip can analyze large quantities of biomolecules in a very short time, enabling rapid and accurate acquisition of biological information from samples. The detection efficiency is hundreds or even thousands of times higher than traditional detection methods. Biochips represent another profound scientific and technological revolution following large-scale integrated circuits. Gene chips are the most mature and commercially available product in biochip technology. Gene chips are developed based on the principle of complementary hybridization of nucleic acid probes. A nucleic acid probe is simply a synthetically produced base sequence. Detectable substances are attached to the probe, and based on the principle of base complementarity, the gene probe is used to identify specific genes in a gene mixture.

[0005] II. Testing Instruments

[0006] Hybridization instruments are widely used analytical instruments in the biological field, employing nucleic acid hybridization technology to detect the presence of known gene sequences in a genome. During use, liquids (hybridization solution, binding solution, sample liquid, etc.) are placed in the hybridization instrument for reaction. The reaction results are determined by the color and state of the reacted sample. Many existing hybridization instruments only provide reaction conditions and requirements; after the reaction, the sample must be manually or mechanically removed for color and state analysis to obtain the detection results, resulting in low detection efficiency. After the reaction, the instrument needs to be cleaned, especially the liquid flow channels, such as the hollow needles fixed inside the hybridization instrument for liquid collection or drainage. Frequent cleaning reduces the detection efficiency of the hybridization instrument and affects its lifespan. Furthermore, it is difficult to thoroughly clean the narrow liquid flow channels, such as the hollow needles, affecting the performance of the hybridization instrument. Summary of the Invention

[0007] To address the aforementioned problems in existing technologies, the purpose of this invention is to provide an integrated biochip machine that combines biochip hybridization and hybridization result reading, automatically completing the biochip hybridization reaction, result display, and acquisition. It boasts high work efficiency, simple and convenient operation, compact structure, flexible use, and long lifespan.

[0008] To achieve the above objectives, the technical solution adopted by the present invention is as follows:

[0009] A biochip integrated device includes a housing and a hybridization section, a reading section, and a drive section disposed within the housing. The hybridization section is provided with a liquid holding assembly and at least one set of liquid suction and discharge assemblies. At least one set of reactors containing biochips is detachably disposed in the hybridization section. The liquid holding assembly is used to hold liquid. Each set of liquid suction and discharge assemblies is connected to a set of reactors. The liquid suction and discharge assemblies apply pressure to the reactor by moving, causing the liquid in the liquid holding assembly to be sucked into the reactor or the liquid in the reactor to be discharged. The drive section transmits drive to the reactor, driving the reactor to move between the hybridization reaction zone and the reading zone. The reading section is used to read the biochip results after hybridization in the reading zone.

[0010] As a further improvement to the above technical solution:

[0011] The reactor has an internal cavity in which the biochip is placed. A needle is fixedly connected to the reactor and is connected to the internal cavity of the reactor. The front and rear panels of the reactor are transparent, and the internal cavity of the reactor can be observed or photographed through the front or rear panels.

[0012] The drive unit transmits drive to all liquid suction and discharge components or all reactors, driving the liquid suction and discharge components to move to apply pressure to the reactor, or to move the reactor between the hybridization reaction zone and the reading zone. The drive unit transmits drive to all liquid suction and discharge components or all reactors at the same time. The drive unit can switch between transmitting drive to liquid suction and discharge components or reactors.

[0013] The liquid suction and discharge assembly includes a piston and a spring. The reactor has two through holes. One end of the piston is located in one through hole or an extension of this through hole, and the other through hole is connected to the needle tube. When the piston moves back and forth, external liquid is sucked into the reactor or liquid in the reactor is discharged. One end of the spring is connected to the piston and the other end is connected to the integrated machine.

[0014] The hybridization unit also has a frame, on which the reactor is detachably mounted. The frame has at least one mounting position for mounting the reactor. The drive unit can be connected to and transmit drive to the frame, or disconnected from the frame. The drive unit drives the frame and the reactor on the frame to move between the hybridization reaction zone and the reading zone. One end of the spring is connected to the piston and the other end is connected to the frame.

[0015] The drive unit is equipped with a drive assembly and a transmission assembly. The drive assembly connects to and transmits drive to the transmission assembly, causing the transmission assembly to move linearly back and forth. The transmission assembly moves to contact and push the piston and compress the spring, or moves to disengage from the piston. The integrated machine also includes a transfer unit, which is mounted on the transmission assembly. The transfer unit includes a connecting rod, which is driven by electromagnetic force to connect or disconnect from the frame.

[0016] The liquid holding assembly contains at least one liquid, and the pipetting assembly is connected to and drives the liquid holding assembly to move.

[0017] The reading unit is equipped with an image acquisition component, which takes pictures of the reactor entering the reading area.

[0018] The hybridization section is also equipped with a linearly movable temperature-controlled heating element. The frame is placed on the temperature-controlled heating element, which heats the reactor on the frame.

[0019] The housing has a power interface and a controller interface. The power-consuming components inside the housing are connected to an external power source through the power interface, and the electrical components inside the housing are electrically connected to an external controller through the controller interface.

[0020] The beneficial effects of this invention are:

[0021] 1) The biochip integrated machine integrates biochip hybridization and hybridization result reading, and can automatically complete the biochip hybridization reaction, display and acquisition of results;

[0022] 2) Multiple reactors can be reacted and the results obtained at one time. The operator only needs to put in the liquid and the reactor, and take out the liquid and the reactor after the reaction is completed. It has high work efficiency, is simple and convenient to operate, and has low requirements for the operator.

[0023] 3) The biochip integrated machine has a compact structure and all components work together, which improves work efficiency and ensures the accuracy of test results;

[0024] 4) The reactor's liquid intake and discharge and the reactor's entry from the hybridization reaction zone into the reading zone are driven by the same motor, eliminating the need for separate motors. This facilitates the compactness and miniaturization of the integrated machine, making it more flexible and convenient to use.

[0025] 5) The reactor and syringe are connected together and installed as a consumable in the integrated machine. After use, they can be directly removed without cleaning the integrated machine, which ensures the performance of the integrated machine and improves its service life. Attached Figure Description

[0026] Figure 1 This is a schematic diagram of the appearance of one embodiment of the present invention.

[0027] Figure 2 This is a schematic diagram of a structure of the present invention with the middle cover plate and the front panel removed.

[0028] Figure 3 yes Figure 2 A structural diagram with handles and shooting auxiliary components removed.

[0029] Figure 4 yes Figure 3 A schematic diagram of the structure without the heat block.

[0030] Figure 5 This is a schematic diagram of the image acquisition component structure according to an embodiment of the present invention.

[0031] Figure 6 This is a schematic diagram of the reactor and syringe structure according to an embodiment of the present invention.

[0032] Figure 7 This is a schematic diagram of the transmission assembly and transfer unit according to an embodiment of the present invention.

[0033] Figure 8 This is a schematic diagram of a sealing strip structure according to an embodiment of the present invention. Detailed Implementation

[0034] The specific embodiments of the present invention will be described in detail below with reference to the accompanying drawings. It should be understood that the specific embodiments described herein are for illustration and explanation only and are not intended to limit the present invention.

[0035] For ease of description, spatial relative terms such as "above," "on top of," "on the upper surface of," "above," etc., are used herein to describe the spatial positional relationship of a device or feature as shown in the figures to other devices or features. It should be understood that spatial relative terms are intended to encompass different orientations in use or operation beyond the orientation of the device as described in the figures. For example, if the device in the figures were inverted, a device described as "above" or "on top of" other devices or structures would subsequently be positioned as "below" or "under" other devices or structures. Thus, the exemplary term "above" can include both "above" and "below." The device may also be positioned in other different ways (rotated 90 degrees or in other orientations), and the spatial relative descriptions used herein will be interpreted accordingly.

[0036] A biochip integrated machine, such as Figures 1-8 As shown, the device includes a housing and a hybridization unit, a transfer unit 8, a drive unit, and a reading unit installed within the housing. The biochip is disposed within a reactor 1', which hybridizes with a liquid in the hybridization reaction zone of the hybridization unit. The transfer unit 8 moves the reactor 1' between the hybridization reaction zone and the reading zone. The reading unit reads the results of the biochip entering the reading zone of the reactor 1'. The drive unit, driven by pressure, draws external liquid into the reactor 1' or discharges liquid from the reactor 1'. The drive unit also drives the transfer unit 8, causing the transfer unit 8 to move the reactor 1' between the hybridization reaction zone and the reading zone.

[0037] The housing includes a lower mounting plate 101, a middle cover plate 102, a rear panel 103, and a front panel 104. These components are connected to form the outer shell of the biochip integrated machine. The lower mounting plate 101 is located at the bottom of the biochip integrated machine. The rear panel 103 and front panel 104 are positioned opposite each other at opposite ends of the lower mounting plate 101. The middle cover plate 102 covers the top surface and two opposite sides of the biochip integrated machine. An upper motherboard 105 for mounting other components is located inside the housing. Preferably, the upper motherboard 105 and the lower mounting plate 101 are arranged parallel and spaced apart. The shape of the upper motherboard 105 can be flexibly designed according to the component arrangement. A power switch and a USB interface are provided on the rear panel 103. The power switch turns the circuit containing the electrical equipment inside the housing on or off. The USB interface can be connected to a computer or other control system to collect data and input control commands.

[0038] The hybridization section includes a liquid-holding assembly 2, a liquid-transfer assembly 3, a frame, a temperature control heating element, a reactor loading and unloading assembly, and at least one set of liquid suction and discharge assemblies. In this embodiment, multiple sets of liquid suction and discharge assemblies are provided.

[0039] The liquid holding assembly 2 is used to hold liquids and includes a test tube tray 201 and a test tube trough 202. The upper surface of the test tube tray 201 has multiple elongated grooves arranged parallel to each other. These grooves are used to hold test tubes containing liquids, and each groove can hold at least one test tube, meaning that multiple liquids can be placed in each groove. The test tube tray 201 is detachably supported on the test tube trough 202.

[0040] The pipetting assembly 3 connects to and drives the liquid-holding assembly 2 to move. Furthermore, the liquid-holding assembly 2 is driven to move reciprocally in a linear motion; during this linear movement, the liquid-holding assembly 2 extends or retracts from the housing. The pipetting assembly 3 is mounted on the lower mounting plate 101. Specifically, the pipetting assembly 3 includes a motor, a pulley drive assembly, and a linear guide rail. The test tube trough 202 is mounted on the linear guide rail, and the motor transmits its drive to the linear guide rail via the pulley drive assembly. The linear guide rail then drives the test tube trough 202 and the test tube tray 201 on the test tube trough 202 to move linearly.

[0041] The linear guide includes a base, a lead screw, and a nut connecting block. The base is fixedly mounted on the lower mounting plate 101, the lead screw is rotatably mounted on the base, and the nut connecting block is screwed to the lead screw. When the lead screw rotates, it drives the nut connecting block to move along the length of the lead screw on the base.

[0042] The first belt drive assembly includes a belt seat and two belt pulleys. The belt seat is fixedly mounted on the lower mounting plate 101. The two belt pulleys are respectively connected to the motor shaft of the first motor and one end of the lead screw. A drive belt is tensioned on the two belt pulleys.

[0043] Reactor 1' has an internal cavity in which the biochip is placed. A needle 2' is connected to reactor 1'. Preferably, reactor 1' and needle 2' are fixedly connected, with one end of needle 2' connected to reactor 1' and the other end extending out. The internal channel of needle 2' communicates with the internal cavity of reactor 1'. The front and rear panels of reactor 1' are transparent and are positioned opposite each other. The internal cavity of reactor 1' can be observed or photographed through either the front or rear panel. During photographing, lighting can be provided from the rear panel, and the image can be taken from the front panel. A through-hole is provided at the upper end of reactor 1', and this through-hole communicates with the internal cavity of reactor 1'.

[0044] The frame includes a reactor frame 402 and a sealing strip 401.

[0045] The reactor frame 402 is used to install and support the reactor 1'. The reactor frame 402 has multiple mounting positions for installing the reactor 1', arranged in a parallel, spaced-apart row. The upper end of the reactor frame 402 also has multiple through holes, which connect to the mounting positions. The reactor 1' is detachably mounted on the mounting position. The reactor frame 402 is detachably mounted on the upper main plate 105, and is located above the liquid-containing assembly 2. After the reactor 1' is installed on the reactor frame 402, the reactor 1' snaps into the mounting position. The internal cavity of the reactor 1', the through holes at the upper end of the reactor 1', and the through holes at the upper end of the reactor frame 402 are sequentially connected. To improve the sealing of the connected channel, a sealing ring can be provided at the connection between the upper end of the reactor 1' and the reactor frame 402. Specifically, the reactor 1' snaps into the mounting position from the side of the reactor frame 402. The transparent front and rear panels of reactor 1' are not obstructed by reactor frame 402, and the corresponding positions of reactor frame 402 are designed to be open for subsequent reading by the reading unit. The open end of the syringe 2' faces downward and extends out of the bottom of reactor frame 402. The lower ends of multiple syringes 2' at multiple mounting positions are respectively connected to multiple grooves on test tube tray 201. Through the operation of the subsequent liquid suction and discharge assembly, the liquid in test tube tray 201 can be drawn into reactor 1' through syringes 2'.

[0046] The sealing strip 401 is detachably connected to the upper end of the reactor frame 402. After connection, the upper end face of the reactor frame 402 and the lower end face of the sealing strip 401 are in contact. The sealing strip 401 includes a strip body 4011 and two horizontal blocks 4012 and 4013 located on the same surface of the strip body 4011. Horizontal blocks 4012 and 4013 are arranged in parallel and spaced apart, with horizontal block 4012 located below horizontal block 4013. Horizontal block 4012 has a row of through holes arranged in parallel and spaced apart, and horizontal block 4013 has a row of through holes arranged in parallel and spaced apart. The through holes on horizontal block 4012 and the through holes on horizontal block 4013 are respectively on the same straight line. The number of through holes on horizontal block 4012, the number of through holes on horizontal block 4013, and the number of mounting positions on the reactor frame 402 are equal. Preferably, the diameter of the through hole on the first horizontal block 4012 is equal to the diameter of the through hole on the second horizontal block 4013. When the sealing strip 401 is connected to the reactor frame 402, the multiple through holes on the first horizontal block 4012 are respectively connected to the multiple through holes at the upper end of the reactor frame 402. To improve the sealing performance of the connected channel, a sealing ring can be provided at the connection between the sealing strip 401 and the reactor frame 402. The strip body 4011 is also provided with a blind hole-shaped connection hole, which, along with the first horizontal block 4012, is located on two sides of the strip body 4011. Preferably, the sealing strip 401 is made of transparent plexiglass.

[0047] A temperature-controlled heating element is used to heat reactor 1', providing the temperature conditions for the reaction in reactor 1'. The temperature-controlled heating element includes a heating block 501, a heating film, wires, and a temperature sensor. The heating block 501 has an internal cavity, with an opening at the top and multiple holes spaced apart at the bottom communicating with the internal cavity. The reactor frame 402 can be inserted into the heating block 501 from the top, while multiple needles 2' on the reactor frame 402 extend out of the heating block 501 after passing through the multiple holes at the bottom. That is, the reactor frame 402 is placed directly in the heating block 501 without needing to connect the two through connecting components. The two ends of the heating block 501 are supported on the upper main plate 105. That is, the reactor frame 402 is not directly supported on the upper main plate 105, but is supported on the upper main plate 105 through the heating block 501. The heating block 501 is covered with a heating film, and wires connect the heating film to an external power source to heat the film. Heat is transferred through the heating block 501 to the liquid in reactor 1'. When reactor frame 402 is placed on the heating block 501, the top of reactor frame 402 is higher than the top of the heating block 501. A temperature sensor is used to detect the temperature of the heating block 501 and sends the detected temperature to the controller.

[0048] The reactor loading / unloading assembly is used to bring the reactor frame 402 out of or back into the housing. The assembly includes a handle 601, two sliders 602, two slide rails 603, and two spring cables 604. The two slide rails 603 are arranged parallel and spaced apart on both sides of the hot block 501, perpendicular to each other. The slide rails 603 are fixedly mounted on the upper main plate 105, and the two sliders 602 can slide linearly along the two slide rails 603 respectively. Both ends of the hot block 501 are connected to and supported on the two sliders 602. Both ends of the handle 601 are connected to and supported on the two sliders 602 or on the two ends of the hot block 501. When the handle 601 is pulled, the hot block 501, reactor frame 402, and sealing strip 401 move linearly via the two sliders 602, causing them to simultaneously extend out of or retract into the housing. The handle 601 is located on the front panel 104.

[0049] To ensure the reactor frame 402 can accurately retract to its initial position and remain stably in place, two spring wires 604 are provided. The two spring wires 604 are arranged in parallel and spaced apart, each connected to one of the two sliders 602. One end of each spring wire 604 is connected to the main board 105, and the other end is connected to one end of a slider 602 or a heating block 501. The spring wires 604 are in a stretched state. When the reactor frame 402 and the heating block 501 are pulled out, the stretched length of the spring wires 604 increases. When the reactor frame 402 and the heating block 501 are retracted to the set position, the heating block 501 or the slider 602 is prevented from retracting further by a limiting component. At this time, the spring wires 604 remain stretched, thus ensuring that the reactor frame 402 and the heating block 501 are stably and reliably positioned in the set position. Preferably, the wires of the temperature-controlled heating element are located inside the spring wires 604, meaning the spring wires 604 serve the dual purpose of energizing the heating block 501 and stabilizing the position of the reactor frame 402.

[0050] The suction / discharge assembly includes a piston 701 and a spring. One end of the piston 701 has a limiting head, the outer diameter of which is larger than the outer diameter of the piston 701. The outer diameter of the piston 701 is not larger than the diameter of the through hole on the first horizontal block 4012 of the sealing strip 401, but the outer diameter of the limiting head of the piston 701 is larger than the diameters of the through holes on both the first horizontal block 4012 and the second horizontal block 4013. The piston 701 can extend into the through hole on the first horizontal block 4012, and the circumferential surface of the piston 701 fits against the wall of the through hole, thereby sealing one end of the through hole on the first horizontal block 4012. One end of piston 701, away from the limiting head, extends into the through hole on horizontal block 4012, while the other end is located between horizontal block 4012 and horizontal block 4013. That is, the limiting head is located between horizontal block 4012 and horizontal block 4013, and the limiting head cannot extend into the through hole of horizontal block 4012 or horizontal block 4013. Multiple pistons 701 of the multiple sets of suction and discharge assemblies extend into multiple through holes on horizontal block 4012, and the multiple pistons 701, the multiple through holes on horizontal block 4012, and the multiple through holes on horizontal block 4013 are all on the same center line. Multiple springs of the multiple sets of suction and discharge assemblies are respectively sleeved on the multiple pistons 701. The springs are located between horizontal block 4012 and horizontal block 4013, sleeved outside the pistons 701. Specifically, one end of the spring is connected to the top of horizontal block 4012, and the other end is connected to the limiting head of piston 701.

[0051] The transfer unit 8 is equipped with a magnet mounting bracket, an electromagnet, a connecting rod, and a return spring. The electromagnet is mounted on the magnet mounting bracket, and the connecting rod is mounted on the magnet mounting bracket via the return spring. One end of the return spring is connected to the connecting rod, and the other end is connected to the magnet mounting bracket. The connecting rod and the lower mounting plate 101 are arranged parallel to each other at intervals. When the electromagnet is energized, it exerts a force on the metal connecting rod, driving the connecting rod to move and insert it into the connecting hole on the pressure strip body 4011 of the sealing pressure strip 401. At the same time, the return spring is stretched. The drive unit drives the magnet mounting bracket to rise and fall, thereby driving the sealing pressure strip 401 and the reactor frame 402 to rise and fall via the connecting rod. After the reactor frame 402 rises, it will detach from the hot block 501. When the electromagnet is de-energized, the connecting rod returns to its original position under the elastic force of the return spring, exiting the sealing pressure strip 401 and disengaging from contact with the sealing pressure strip 401.

[0052] The drive unit includes a drive assembly 9 and a transmission assembly.

[0053] The drive assembly 9 can connect to and drive the liquid suction and discharge assembly to operate, sucking in or discharging liquid from reactor 1'. Simultaneously, the drive assembly 9 can also drive the sealing strip 401 and reactor frame 402 to rise and fall. The drive unit transmits drive to all liquid suction and discharge assemblies or transfer units 8; that is, the drive assembly 9 does not transmit drive to both the liquid suction and discharge assemblies and the transfer units 8 simultaneously, but only drives the liquid suction and discharge assemblies or the transfer units 8 at any given time.

[0054] The drive assembly 9 includes a second motor, a module fixing plate, and a second linear guide rail. The transmission assembly includes a transmission plate 1001 and multiple transmission rods 1002. The module fixing plate is fixedly mounted on the upper main plate 105. The second linear guide rail is mounted on the module fixing plate. The transmission plate 1001 is connected to the second linear guide rail. The second motor transmits drive to the transmission plate 1001 through the second linear guide rail, causing the transmission plate 1001 to rise or fall.

[0055] Multiple transmission rods 1002 are arranged in a parallel row at intervals. One end of each transmission rod 1002 is fixedly connected to the transmission plate 1001, and the other end extends downwards. The number of transmission rods 1002 is the same as the number of pistons 701. The outer diameter of each transmission rod 1002 is no larger than the diameter of the through hole on the second horizontal block 4013. The multiple transmission rods 1002 share a common centerline with the multiple through holes on the second horizontal block 4013. When the transmission plate 1001 descends, the multiple transmission rods 1002 are driven down and pass through the multiple through holes on the second horizontal block 4013, and then contact one end of the limiting head of each piston 701. As the transmission rod 1002 continues to descend, it presses down on the piston 701 it contacts, compressing the spring. When the transmission rod 1002 rises, the piston 701 will be driven to rise by the spring. When the transmission rod 1002 enters the through hole on the second horizontal block 4013, the piston 701 will disengage from the transmission rod 1002 and cannot continue to move upward because the limiting head of the piston 701 cannot enter the through hole on the second horizontal block 4013.

[0056] The magnet mounting bracket of the transfer unit 8 is fixedly installed on the bottom surface of the transmission plate 1001, that is, the transfer unit 8 is driven to rise or fall by the drive assembly 9.

[0057] The image acquisition unit is equipped with an image acquisition component that captures images of the reactor 1' after the reaction and transmits the captured information to the control system electrically connected to it. The image acquisition component includes a camera assembly 1201, a slide rail 1202, a camera drive unit 1203, and an image acquisition auxiliary component. The slide rail 1202 is mounted on the upper main board 105, and its length direction is parallel to the arrangement direction of the reactors 1' on the reactor frame 402. The camera assembly 1201 is slidably mounted on the slide rail 1202 and is used to capture images of the reactors 1'. The camera drive unit 1203 connects to and drives the camera assembly 1201 to move along the slide rail 1202 to capture images of the multiple reactors 1' on the reactor frame 402. The image acquisition auxiliary component includes a backlight, a light shield, etc., and the backlight and light shield provide suitable lighting conditions for image capture. Both ends of the backlight and the light shield are connected to the heat block 501 or the slide rail 603. That is, when the handle 601 is pulled, the backlight and the light shield move synchronously with the heat block 501 and the reactor frame 402.

[0058] All electrical components, including motors, image acquisition components, electromagnet circuit switches, and heating film 501 circuit switches, are electrically connected to the external control system via USB interfaces.

[0059] The method of use and working process of this invention are as follows:

[0060] Step 1: Install reactor 1' onto reactor rack 402.

[0061] First, pull the handle 601. The handle 601 drives the hot block 501 or the slider 602, so that the hot block 501 moves along the slide rail 603 through the slider 602 and moves out of the shell. At the same time, the hot block 501 drives the reactor frame 402 placed on it, the sealing strip 401 connected to the reactor frame 402, and the liquid suction and discharge assembly on the sealing strip 401 to extend synchronously.

[0062] Then, remove the sealing strip 401 and the reactor frame 402 connected to it, and insert multiple reactors 1' into the side of the reactor frame 402 respectively, so that the reactors 1' are stably installed in the mounting position of the reactor frame 402, and the needle tube 2' extends downwards out of the bottom of the reactor frame 402.

[0063] Finally, the reactor frame 402 and sealing strip 401, with reactor 1' installed, are placed inside the hot block 501, with the reactor frame 402 facing downwards. Multiple needles 2' pass through multiple holes at the lower end of the hot block 501 and extend out of the hot block 501. The pull handle 601 is retracted, and the spring cable 604 drives the slider 602, hot block 501, reactor frame 402, and sealing strip 401 back to their initial positions, where they are stably held in place by the tension of the spring cable 604.

[0064] Step 2: Connect the USB port on the rear panel 103 of the biochip integrated machine to a computer or other control system, and turn on the power.

[0065] Step 3: Place the test tubes containing liquid into the test tube tray 201.

[0066] First, the control system starts motor one, which drives the lead screw of linear guide rail one to rotate through the pulley transmission group one. The lead screw drives the nut connecting block to move along its length direction. The nut connecting block drives the test tube trough 202 and test tube trough tray 201 connected to it to move, so that the test tube trough 202 and test tube trough tray 201 extend out of the shell.

[0067] Then, the test tubes containing liquid are placed on the grooves of the test tube tray 201. Preferably, a liquid box is provided that mates with the grooves of the test tube tray 201. The liquid box includes a box body and a test tube rack. The test tube rack is mounted on the box body and has multiple test tubes, each used to hold a different liquid. The test tube rack also has a through hole communicating with the box body, which can also be used to receive waste liquid. The arrangement direction of the multiple test tubes is parallel to the moving direction of the test tube tray 201.

[0068] Step 4: Start motor 1 to drive test tube tank 202 and test tube tank tray 201 to run according to the set program. Reactor 1' simultaneously performs liquid absorption and discharge, and heating block 501 is energized to raise the temperature.

[0069] In this step, the test tube tray 201 moves in a step-by-step manner. The fourth step includes the following steps:

[0070] Step S1: The transfer unit 8 lifts the reactor frame 402 so that the needle 2' does not interfere with the movement of the test tubes on the test tube tray 201.

[0071] First, the circuit containing the electromagnet is turned on. At this time, the connecting hole on the sealing strip 401 is aligned with the connecting rod of the transfer part 8 on the transmission plate 1001, which is in its initial position, and the transmission rod 1002 does not contact the sealing strip 401. After the electromagnet is energized, it generates a force with the connecting rod, and the connecting rod is driven to insert into the connecting hole on the strip body 4011 of the sealing strip 401, keeping the electromagnet energized.

[0072] Then, the second motor is started, and the power of the second motor is transmitted to the transmission plate 1001 through the second linear guide rail. The transmission plate 1001 is driven to rise by the second linear guide rail, which in turn drives the connecting rod on the transmission plate 1001 to rise. The connecting rod drives the sealing strip 401, the reactor frame 402, and the reactor 1' to rise synchronously.

[0073] Step S2: The test tube tray 201 moves until a test tube containing liquid is directly below the syringe 2', at which point the test tube tray 201 stops moving.

[0074] Step S3: The transfer unit 8 drives the reactor frame 402 to descend, so that the needle 2' is inserted into the test tube.

[0075] In this step, the reverse start motor 2 is activated, and the transmission plate 1001 and the connecting rod are driven to descend. The connecting rod drives the sealing strip 401, the reactor frame 402, and the reactor 1' to descend synchronously, so that the reactor frame 402 re-enters and is supported on the hot block 501.

[0076] Step S4: The liquid suction and discharge assembly is activated, and the syringe 2' draws the liquid in the test tube into the internal cavity of the reactor 1'.

[0077] In this step, firstly, the control system starts motor two, transmitting its power to transmission plate 1001 via linear guide rail two. At this time, the circuit connecting the electromagnet is disconnected, and the connecting rod of transfer part 8 is not connected to the sealing strip 401. Transmission plate 1001 is driven down by linear guide rail two. Multiple transmission rods 1002 on transmission plate 1001 extend into and pass through multiple through holes on horizontal block two 4013. When multiple transmission rods 1002 contact multiple pistons 701, the pistons 701 are pressed down and descend along multiple through holes on horizontal block one 4012. At this time, the spring outside the piston 701 is compressed. As the piston 701 descends, the gas in the through hole below the piston 701 on horizontal block one 4012 and in the reactor 1' chamber is discharged outwards.

[0078] Then, motor two is started in reverse, transmitting its power to transmission plate 1001 via linear guide rail two. Transmission plate 1001 is driven upward by linear guide rail two, and piston 701 rises under the force of the spring until its limiting head contacts the bottom of horizontal block two 4013, preventing further upward movement. Since the spring is still compressed at this point, it stably maintains piston 701's current state. During piston 701's ascent, the liquid in the test tube is drawn into the cavity of reactor 1' through syringe 2', similar to the principle of a syringe. Transmission plate 1001 continues to rise to its initial position.

[0079] Step S5: The transfer unit 8 lifts the reactor frame 402 and moves the test tube tray 201. When the opening of the cavity for holding the waste liquid is below the syringe 2', the test tube tray 201 stops moving.

[0080] Step S6: The liquid suction and discharge assembly is activated, and the liquid in the reactor 1' chamber is discharged. The operation of the liquid suction and discharge assembly in this step is the same as in step S4.

[0081] Step S7: Repeat steps S1 to S6 to draw liquid from another test tube into syringe 2'.

[0082] The reaction is complete once reactor 1' has sequentially drawn in the liquids from each test tube. After each liquid draw, reactor 1' completes a process, such as hybridization, washing, or coloring.

[0083] During the reaction, the heating block 501 transfers heat to the reactor 1'. Specifically, the circuit outside the heating block 501 is activated, and the heating film is heated. The heat is transferred through the heating block 501 to the reactor 1' inside the heating block 501, providing the necessary reaction conditions for the liquid in the reactor 1'. During the reaction, the temperature of the heating block 501 is detected by a temperature sensor. When the detected temperature of the heating block 501 is higher than a set range, the controller disconnects the circuit containing the heating film, and the heating block 501 stops heating; when the detected temperature of the heating block 501 is lower than the set range, the controller connects the circuit containing the heating film, and the heating film heats up.

[0084] Step 5: After the reaction is complete, take pictures or videos of each reactor 1' and save them.

[0085] First, the transfer unit 8 lifts the reactor frame 402, exposing the reactor 1' and placing it at the same height as the camera assembly 1201.

[0086] Then, the camera driver 1203 is activated, causing the camera driver 1203 to move the camera assembly 1201 along the slide rail 1202, and to photograph each reactor 1' in sequence.

[0087] Finally, the transfer unit 8 lowers the reactor frame 402.

[0088] Step 6: Remove the liquid box from the test tube tray 201 and dismantle reactor 1'.

[0089] Finally, it is necessary to state that the above embodiments are only used to further illustrate the technical solution of the present invention in detail, and should not be construed as limiting the scope of protection of the present invention. Any non-essential improvements and adjustments made by those skilled in the art based on the above content of the present invention shall fall within the scope of protection of the present invention.

Claims

1. A biochip integrated machine, characterized in that, The device includes a shell and a hybridization unit, a reading unit, and a driving unit disposed within the shell. The hybridization unit is provided with a liquid holding assembly (2) and at least one set of liquid suction and discharge assemblies. At least one set of reactors (1') containing biochips is detachably disposed within the hybridization unit. The reactors (1') have an internal cavity in which the biochips are disposed. A needle (2') is fixedly connected to the reactors (1'), and the needle (2') communicates with the internal cavity of the reactors (1'). The front and rear panels of the reactors (1') are transparent, and the internal cavity of the reactors (1') can be observed or photographed through the front or rear panels. The liquid holding assembly (2) is used to hold liquid. Each set of liquid suction and discharge assemblies is connected to a set of reactors (1'). The liquid suction and discharge assembly includes a piston (701) and a spring. The reactors (1') have two through holes, and one end of the piston (701) is located in one of the through holes or an extension of the through hole. The long hole is connected to another through hole and the needle (2'). When the piston (701) moves back and forth, the external liquid is drawn into the reactor (1') or the liquid in the reactor (1') is discharged. The hybridization part is also equipped with a frame. The reactor (1') is detachably installed on the frame. One end of the spring is connected to the piston (701) and the other end is connected to the frame. The drive unit transmits the drive to all the liquid suction and discharge components or all the reactors (1'), driving the liquid suction and discharge components to move to apply pressure to the reactor (1') or to move the reactor (1') between the hybridization reaction zone and the reading zone. The drive unit transmits the drive to all the liquid suction and discharge components at the same time, or transmits the drive to all the reactors (1') at the same time. The drive unit can switch the drive to the liquid suction and discharge components or the reactor (1'). The reading unit is used to read the results of the biochip after hybridization in the reading zone.

2. The all-in-one machine according to claim 1, characterized in that: The frame is provided with at least one mounting position for installing the reactor (1'). The drive unit can be connected to and transmit drive to the frame, or disconnected from the frame. The drive unit drives the frame and the reactor (1') on the frame to move between the hybridization reaction zone and the reading zone.

3. The all-in-one machine according to claim 2, characterized in that: The drive unit is provided with a drive assembly (9) and a transmission assembly. The drive assembly (9) is connected to and transmits drive to the transmission assembly, causing the transmission assembly to move linearly back and forth. The transmission assembly moves to contact and push the piston (701) and compress the spring, or moves to disengage from contact with the piston (701). The integrated machine also includes a transfer unit (8), which is mounted on the transmission assembly. The transfer unit (8) includes a connecting rod, which is driven by electromagnetic force to connect or disconnect from the frame.

4. The all-in-one machine according to claim 1, characterized in that: The liquid holding assembly (2) holds at least one liquid, and the hybridization part is also provided with a pipetting assembly (3), which is connected to and drives the liquid holding assembly (2) to move.

5. The all-in-one machine according to claim 1, characterized in that: The reading unit is equipped with an image acquisition component, which takes pictures of the reactor (1') entering the reading area.

6. The all-in-one machine according to claim 2, characterized in that: The hybridization section is also equipped with a linearly movable temperature control heating element. The frame is placed on the temperature control heating element, which heats the reactor (1') on the frame placed on it.

7. The all-in-one machine according to claim 1, characterized in that: The housing has a power interface and a controller interface. The power-consuming components inside the housing are connected to an external power source through the power interface, and the electrical components inside the housing are electrically connected to an external controller through the controller interface.

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