A method for improving the activity of southern Chinese cypress seeds and promoting their germination

Abstract

The present invention discloses a method for improving the activity of seeds of southern China and promoting their germination, comprising the following steps: (1) seed activity detection: exploring the correlation between the content of soluble sugar, lipid and protein in seeds and seed activity and establishing a linear regression formula to obtain seed activity; (2) seed activity treatment: treating the low-activity southern China seeds with high-voltage direct current electrostatic treatment, and then performing wet balance treatment with steam liquid, hydration treatment liquid and convective drying treatment for multiple cycles. The method of the present invention treats the low-activity seeds of southern China with high-voltage direct current electrostatic treatment and wet balance treatment with steam liquid, hydration treatment liquid and convective drying for multiple cycles, thereby improving the activity of southern China seeds, promoting the germination of southern China and shortening the germination time.

Description

Technical Field

[0001] The invention relates to the technical field of plant seed treatment, in particular to a method for improving the activity of southern cypress seeds and promoting their germination. Background Art

[0002] The southern Chinese yew is a perennial evergreen tree of the genus Elaeagnus, a tree species endemic to China. It is mainly distributed in southeastern Yunnan, Guizhou, northwestern and eastern Guangxi, northern Guangdong, southern Hunan, southwestern Jiangxi, and southwestern Zhejiang. The southern Chinese yew has an elegant and beautiful tree shape and can be used as a garden greening tree species. The bark of the southern Chinese yew has medicinal effects of clearing rashes, reducing swelling, and setting bones. The southern Chinese yew has high economic value, but due to the low renewal rate of the southern Chinese yew population, it has become an endangered species and has been included in the "Red Data of Chinese Plants" and the "Atlas of Rare and Endangered Plants in Guangdong Province". Population renewal is related to the number of highly active seeds in the seed bank, seed germination rate, and low seedling renewal rate. By observing the seed rain in the natural forest of Jiangnan Oilseed Fir in Yachang Forest Farm, Baise City, Guangxi from 2018 to 2021, the high-vitality seeds in 2018-2021 were 40.53%, 50.33%, 49.66%, and 48.31%, respectively. Moreover, these highly vigorous seeds will gradually lose their seed vitality with the passage of storage time. Before the arrival of seed rain in the second year, the vitality of all seeds will drop to 0. Jiangnan Oilseed Fir seeds have problems such as few highly active seeds and rapid decline in seed activity, resulting in short storage time. Improving seed activity and delaying the decline in seed activity will promote the germination of Jiangnan Oilseed Fir seeds, thereby increasing the population size of Jiangnan Oilseed Fir and improving the current low renewal of Jiangnan Oilseed Fir population.

[0003] The information disclosed in this background technology section is only intended to enhance the understanding of the overall background of the invention and should not be regarded as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to a person skilled in the art. Summary of the invention

[0004] The purpose of the present invention is to provide a method for improving the activity of southern Chinese cypress seeds and promoting their germination, so as to overcome the problems of rapid decline in the activity of southern Chinese cypress seeds and small number of highly active seeds, promote the germination of southern Chinese cypress seeds and thus increase the population of southern Chinese cypress.

[0005] To achieve the above object, the present invention provides a method for improving the activity of Lutetia chinensis seeds and promoting their germination, which is characterized by comprising the following steps:

[0006] (1) Seed activity detection: The correlation between the content of soluble sugar, lipid and protein in the seeds of Ludwigia truncatum and the seed activity was explored, and a linear regression formula between the content of soluble sugar, lipid and protein and the seed activity was established. The content of soluble sugar, lipid and protein was substituted into the linear regression formula to obtain the seed activity of Ludwigia truncatum. When the number of Ludwigia truncatum seeds with low activity in the sample accounted for ≥60% of the total number of samples, all seeds were treated to improve the seed activity, wherein:

[0007] The linear regression formula of the content of soluble sugar, lipid, protein and seed activity is: seed activity = 16.945 + 0.912 * soluble sugar + 1.390 * lipid + 1.334 * protein, the model R square value is 0.925, and the model R square value indicates that soluble sugar, lipid, protein can explain 92.5% of the change in seed activity.

[0008] The low-activity seeds of the southern Chinese cypress refer to seeds of the southern Chinese cypress whose activity is less than 60 obtained by substituting the contents of soluble sugar, lipid and protein obtained by testing into the linear regression formula;

[0009] (2) Seed activity treatment: treating the low-activity southern cypress seeds with high-voltage direct current electrostatic treatment, and then performing a wet balance treatment with a steam liquid, hydration with a hydration treatment liquid, and convection drying treatment for multiple cycles, wherein the steam liquid composition includes humic acid, chitin, and sodium silicate, and the hydration treatment liquid is anhydrous acetone dissolved with cysteine, potassium iodide, p-hydroxybenzoic acid, oxalic acid, malic acid, and cross-linked sodium polyacrylate;

[0010] Preferably, in the above technical solution, the concentration range of the steam liquid in step (2) is 50-65 ml humic acid, 8-14 g chitosan, and 17-21 g sodium silicate.

[0011] Preferably, in the above technical solution, the 0-5 cm withered materials under the southern Chinese fir forest are mechanically broken, soaked and decomposed with cellulose hydrolase and pectin hydrolase, and then filtered to obtain the product; wherein,

[0012] The withered substance: cellulose hydrolase: pectin hydrolase = 76-83:10-14:7-10.

[0013] Preferably, in the above technical solution, the hydration treatment liquid components in step (2) include one or more of anhydrous acetone, cysteine, potassium iodide, p-hydroxybenzoic acid, oxalic acid, malic acid, and cross-linked sodium polyacrylate.

[0014] Preferably, in the above technical solution, the method for obtaining the hydration treatment solution in step (2) is to use 150-220 ml of anhydrous acetone to dissolve 20-30 ml of cysteine, 18-23 ml of potassium iodide, 6-11 ml of p-hydroxybenzoic acid, 4-8 ml of oxalic acid, 12-16 ml of malic acid, and 50-64 ml of cross-linked sodium polyacrylate.

[0015] Preferably, in the above technical solution, the high-voltage direct current electrostatic treatment in step (2) is to place the low-activity seeds in an electrostatic field with a corona circle diameter of 0.4-0.7 mm to generate a high-voltage direct current of 150-320 KV / m for 6-12 minutes.

[0016] Preferably, in the above technical solution, the wet balance treatment method in step (2) is to treat the low-activity seeds after high-voltage direct current electrostatic treatment with steam liquid at a temperature of 18-24° C. for 24-48 hours.

[0017] Preferably, in the above technical solution, the hydration treatment method in step (2) is to treat the low-activity seeds after wet balance treatment with a hydration treatment solution at a temperature of 38-45° C. for 30-50 minutes.

[0018] Preferably, in the above technical solution, the convection drying method in step (2) is to use hot air flow to quickly dry the low-activity seeds after hydration treatment to a moisture content of 13.09-15.4g in a convection manner.

[0019] Preferably, in the above technical solution, the number of cycles of the wet balance treatment-hydration-convection drying treatment in step (2) is 2-4 times.

[0020] Compared with the prior art, the present invention has the following beneficial effects:

[0021] (1) The method of the present invention improves the seed activity of the seeds of the southern Chinese yew tree in a targeted manner by sampling and testing the seed activity of the seeds of the southern Chinese yew tree, and then causes changes in the internal structure and metabolic process of the low-activity southern Chinese yew tree seeds through high-voltage direct current electrostatic treatment, wet balance treatment-hydration-convection drying treatment, changes in the integrity and metabolic process of the membrane, and partially repairs the integrity and damage of the membrane, thereby improving the activity of the seeds of the southern Chinese yew tree.

[0022] (2) The humic acid in the steam liquid used for wet balance treatment is obtained from the decomposed litter of the Chinese fir forest. The litter of the Chinese fir forest contains microorganisms that stimulate the activity of Chinese fir seeds and substances produced by microbial decomposition. The components of the hydration treatment liquid have the function of controlling the free radical chain reaction of seeds and can prolong the life of seeds in a non-hydrated state. The hot air flow dries the seeds by convection and contacts the seeds by convection, so that part of the free water in the seeds is converted into bound water, thereby improving the stress resistance of the Chinese fir seeds.

[0023] (3) After the dead wood is mechanically broken up, the cellulose hydrolase and the gelatin hydrolase accelerate the decomposition of the dead wood and make the dead wood decompose more thoroughly, resulting in less humic acid bacteria and more nutrients. DETAILED DESCRIPTION

[0024] The specific implementation modes of the present invention are described in detail below in conjunction with specific embodiments, but it should be understood that the protection scope of the present invention is not limited by the specific implementation modes.

[0025] To explore the relationship between the content of soluble sugar, lipid and protein in seeds and seed activity:

[0026] Since the germination speed of seeds is a way to reflect the activity of seeds, 200 seeds of Chinese fir were selected for germination test. The seeds with fast germination speed were selected, and the soluble sugar content in the seeds was determined by the anthrone method, the protein content in the seeds was determined by the Coomassie Brilliant Blue method, and the lipid content was obtained by Soxhlet extraction.

[0027] Table 1 Linear regression analysis results (n=20)

[0028]

[0029] Dependent variable: Seed viability

[0030] *p<0.05 **p<0.01

[0031] As shown in Table 1, the correlation formula between the content of soluble sugar, lipid and protein and the activity of seeds of Chinese fir is: seed activity = 16.945 + 0.912 * soluble sugar + 1.390 * lipid + 1.334 * protein. The model R square value is 0.925, which means that soluble sugar, lipid and protein can explain 92.5% of the variation of seed activity. Therefore, the seed activity can be indirectly obtained by detecting the soluble sugar content, lipid content and protein content in Chinese fir seeds.

[0032] Example 1

[0033] A method for improving the activity of southern Chinese cypress seeds and promoting their germination, comprising the following steps:

[0034] (1) Seed activity test: 100 seeds of the southern Chinese cypress were randomly selected from the seed pile to test the contents of soluble sugar, lipid and protein in the seeds. The seed activity of the cypress was obtained by substituting them into the seed activity formula. The number of seeds with low seed activity in the obtained samples accounted for 76% of the total number of samples.

[0035] (2) Seed activity treatment: Low-activity seeds of Elaeagnus chinensis were placed in an electrostatic field with a corona circle diameter of 0.7 mm, and treated in a high-voltage direct current of 320 kV / m generated by the electrostatic field for 6 minutes. 152 g of 1 cm thick dead material under the Elaeagnus chinensis forest was mechanically crushed, and 28 g of cellulose hydrolase and 20 g of pectin hydrolase were added to soak and compost, and then filtered to obtain humic acid. The steam liquid for wet balance treatment was made of 200 g of humic acid, 14 g of chitin, and 21 g of sodium silicate. The seeds of Elaeagnus chinensis were wet balanced with the steam liquid at 18°C ​​for 48 hours. The hydration treatment solution required for the hydration treatment is prepared by dissolving 30 ml of cysteine, 23 ml of potassium iodide, 11 ml of p-hydroxybenzoic acid, 8 ml of oxalic acid, 16 ml of malic acid, and 64 ml of cross-linked sodium polyacrylate in 220 ml of anhydrous acetone. The hydration treatment solution is heated to 40°C and the low-activity seeds obtained by the wet balance treatment are treated for 40 minutes. The low-activity seeds after the hydration treatment are quickly dried to a water content of 13.09 g by convection using a 65°C hot air flow. The wet balance treatment-hydration-convection drying treatment is repeated 4 times.

[0036] Example 2

[0037] A method for improving the activity of southern Chinese cypress seeds and promoting their germination, comprising the following steps:

[0038] (1) Seed activity test: 100 seeds of the southern Chinese cypress were randomly selected from the seed pile to test the contents of soluble sugar, lipid and protein in the seeds. The seed activity of the cypress was obtained by substituting them into the seed activity formula. The number of seeds with low seed activity in the obtained samples accounted for 85% of the total number of samples.

[0039] (2) Seed activity treatment: Low-activity seeds of Elaeagnus chinensis were placed in an electrostatic field with a corona circle diameter of 0.6 mm, and the seeds were treated in a high-voltage direct current of 250 kV / m generated by the electrostatic field for 8 minutes. 156 g of 2 cm thick dead materials under the Elaeagnus chinensis forest were mechanically crushed, and 24 g of cellulose hydrolase and 20 g of pectin hydrolase were added to soak and compost, and then filtered to obtain humic acid. The steam liquid for wet balance treatment was made of 200 g of humic acid, 11 g of chitin, and 19 g of sodium silicate. The steam liquid was used to wet balance the Elaeagnus chinensis seeds at 20°C for 36 hours. The hydration treatment solution required for hydration treatment is prepared by dissolving 25 ml of cysteine, 21 ml of potassium iodide, 9 ml of p-hydroxybenzoic acid, 6 ml of oxalic acid, 14 ml of malic acid, and 59 ml of cross-linked sodium polyacrylate in 200 ml of anhydrous acetone. The hydration treatment solution is heated to 43°C and the low-activity seeds obtained by wet balance treatment are treated for 45 minutes. The low-activity seeds after hydration treatment are quickly dried to a moisture content of 13.4 g by convection using a 50°C hot air flow. The wet balance treatment-hydration-convection drying treatment is repeated 4 times.

[0040] Example 3

[0041] A method for improving the activity of southern Chinese cypress seeds and promoting their germination, comprising the following steps:

[0042] (1) Seed activity test: 100 seeds of the southern Chinese cypress were randomly selected from the seed pile to test the contents of soluble sugar, lipid and protein in the seeds. The activity of the seeds was obtained by substituting them into the seed activity formula. The number of seeds with low seed activity in the obtained samples accounted for 82% of the total number of samples.

[0043] (2) Seed activity treatment: Low-activity seeds of Elaeagnus chinensis were placed in an electrostatic field with a corona circle diameter of 0.5 mm. The seeds were treated in a high-voltage direct current of 200 kV / m generated by the electrostatic field for 10 minutes. 166 g of 3 cm thick dead materials under the Elaeagnus chinensis forest were mechanically crushed, and 20 g of cellulose hydrolase and 14 g of pectin hydrolase were added to the dead materials. Humic acid was obtained by filtering after soaking and composting. The steam liquid for wet balance treatment was made of 200 g of humic acid, 8 g of chitin, and 17 g of sodium silicate. The seeds of Elaeagnus chinensis were wet balanced with the steam liquid at 24°C for 24 hours. The hydration treatment solution required for hydration treatment is prepared by dissolving 20ml of cysteine, 18ml of potassium iodide, 6ml of p-hydroxybenzoic acid, 4ml of oxalic acid, 12ml of malic acid, and 55ml of cross-linked sodium polyacrylate in 180ml of anhydrous acetone. The hydration treatment solution is heated to 45°C and the low-activity seeds obtained by wet balance treatment are treated for 30 minutes. The low-activity seeds after hydration treatment are quickly dried to a moisture content of 14.4g by convection using a 55°C hot air flow. The wet balance treatment-hydration-convection drying treatment is repeated 3 times.

[0044] Example 4

[0045] A method for improving the activity of southern Chinese cypress seeds and promoting their germination, comprising the following steps:

[0046] (1) Seed activity test: 100 seeds of the southern Chinese cypress were randomly selected from the seed pile to test the contents of soluble sugar, lipid and protein in the seeds. The activity of the seeds was obtained by substituting them into the seed activity formula. The number of seeds with low seed activity in the obtained samples accounted for 89% of the total number of samples.

[0047] (2) Seed activity treatment: Low-activity seeds of Elaeagnus truncatula were placed in an electrostatic field with a corona circle diameter of 0.4 mm. The seeds were treated for 12 minutes in a high-voltage direct current of 150 kV / m generated by the electrostatic field. 120 g of 4 cm thick dead material under the Elaeagnus truncatula forest was mechanically crushed. 18 g of cellulose hydrolase and 12 g of pectin hydrolase were added to the dead material, which was soaked and composted, and then filtered to obtain humic acid. The steam liquid for wet balance treatment was made of 150 g of humic acid, 14 g of chitin, and 21 g of sodium silicate. The steam liquid was used to wet balance the Elaeagnus truncatula seeds at 24°C for 24 hours. The hydration treatment solution required for hydration treatment is prepared by dissolving 20ml of cysteine, 18ml of potassium iodide, 6ml of p-hydroxybenzoic acid, 4ml of oxalic acid, 12ml of malic acid, and 53ml of cross-linked sodium polyacrylate in 150ml of anhydrous acetone. The hydration treatment solution is heated to 38°C and the low-activity seeds obtained by wet balance treatment are treated for 30 minutes. The low-activity seeds after hydration treatment are quickly dried to a moisture content of 14.4g by convection using a 55°C hot air flow. The wet balance treatment-hydration-convection drying treatment is repeated 3 times.

[0048] Example 5

[0049] A method for improving the activity of southern Chinese cypress seeds and promoting their germination, comprising the following steps:

[0050] (1) Seed activity test: 100 seeds of the southern Chinese cypress were randomly selected from the seed pile to test the contents of soluble sugar, lipid and protein in the seeds. The activity of the seeds was obtained by substituting them into the seed activity formula. The number of seeds with low seed activity in the obtained samples accounted for 89% of the total number of samples.

[0051] (2) Seed activity treatment: Low-activity seeds of Elaeagnus truncatula were placed in an electrostatic field with a corona circle diameter of 0.7 mm. The seeds were treated in a high-voltage direct current of 320 kV / m generated by the electrostatic field for 6 minutes. 124.5 g of 5 cm thick dead material under the Elaeagnus truncatula forest was mechanically crushed, and 15 g of cellulose hydrolase and 10.5 g of pectin hydrolase were added to the dead material, which was soaked and composted, and then filtered to obtain humic acid. The steam liquid for wet balance treatment was made of 150 g of humic acid, 11 g of chitin, and 19 g of sodium silicate. The steam liquid was used to wet balance the Elaeagnus truncatula seeds at 20°C for 36 hours. The hydration treatment solution required for hydration treatment is prepared by dissolving 30 ml of cysteine, 23 ml of potassium iodide, 11 ml of p-hydroxybenzoic acid, 8 ml of oxalic acid, 16 ml of malic acid, and 50 ml of cross-linked sodium polyacrylate in 200 ml of anhydrous acetone. The hydration treatment solution is heated to 38°C and the low-activity seeds obtained by wet balance treatment are treated for 35 minutes. The low-activity seeds after hydration treatment are quickly dried to a moisture content of 15.4 g by convection using a 60°C hot air flow. The wet balance treatment-hydration-convection drying treatment is repeated 3 times.

[0052] Example 6

[0053] A method for improving the activity of southern Chinese cypress seeds and promoting their germination, comprising the following steps:

[0054] (1) Seed activity test: 100 seeds of the southern Chinese cypress were randomly selected from the seed pile to test the contents of soluble sugar, lipid and protein in the seeds. The seed activity of the cypress was obtained by substituting them into the seed activity formula. The number of seeds with low seed activity in the obtained samples accounted for 79% of the total number of samples.

[0055] (2) Seed activity treatment: Low-activity seeds of Elaeagnus chinensis were placed in an electrostatic field with a corona circle diameter of 0.4 mm. The seeds were treated for 12 minutes in a high-voltage direct current of 150 kV / m generated by the electrostatic field. 114 g of 5 cm thick dead material under the Elaeagnus chinensis forest was mechanically crushed. 21 g of cellulose hydrolase and 15 g of pectin hydrolase were added to the dead material, which was soaked and composted, and then filtered to obtain humic acid. The steam liquid for wet balance treatment was made of 150 g of humic acid, 8 g of chitin, and 17 g of sodium silicate. The steam liquid was used to wet balance the Elaeagnus chinensis seeds at 18°C ​​for 48 hours. The hydration treatment solution required for hydration treatment is prepared by dissolving 25 ml of cysteine, 21 ml of potassium iodide, 9 ml of p-hydroxybenzoic acid, 6 ml of oxalic acid, 14 ml of malic acid, and 59 ml of cross-linked sodium polyacrylate in 180 ml of anhydrous acetone. The hydration treatment solution is heated to 38°C and the low-activity seeds obtained by wet balance treatment are treated for 35 minutes. The low-activity seeds after hydration treatment are quickly dried to a moisture content of 15.4 g by convection using a 65°C hot air flow. The wet balance treatment-hydration-convection drying treatment is repeated 3 times.

[0056] Comparative Example 1

[0057] It is basically the same as Example 1, except that the composition of the vapor liquid in the wet balance treatment in step (2) is different.

[0058] (2) Seed activity treatment: Low-activity seeds of southern Chinese yew were placed in an electrostatic field with a corona diameter of 0.7 mm, and treated in a high-voltage direct current of 320 kV / m generated by the electrostatic field for 6 minutes. The steam liquid for wet balance treatment was made of 14 g chitin and 21 g sodium silicate, and the seeds were wet balanced for 48 hours using the steam liquid at 18°C. The hydration treatment liquid required for hydration treatment was prepared by dissolving 30 ml cysteine, 23 ml potassium iodide, 11 ml p-hydroxybenzoic acid, 8 ml oxalic acid, 16 ml malic acid, and 64 ml cross-linked sodium polyacrylate in 220 ml anhydrous acetone. The hydration treatment liquid was heated to 40°C and treated for 40 minutes with the low-activity seeds obtained by wet balance treatment. The low-activity seeds after hydration treatment were quickly dried to a water content of 13.09 g by convection using a 65°C hot air flow. Four cycles of wet balance treatment-hydration-convection drying were performed.

[0059] Comparative Example 2

[0060] The method is basically the same as Example 1, except that the proportions of the components of the hydration treatment liquid in step (2) are different.

[0061] (2) Seed activity treatment: Low-activity seeds of Elaeagnus chinensis were placed in an electrostatic field with a corona circle diameter of 0.7 mm. The seeds were treated in a high-voltage direct current of 320 kV / m generated by the electrostatic field for 6 minutes. 152 g of 1 cm thick fallen leaves under the Elaeagnus chinensis forest were mechanically crushed and filtered to obtain humic acid after decomposition. The steam liquid for wet balance treatment was made of 200 g humic acid, 14 g chitin, and 21 g sodium silicate. The steam liquid was used to wet balance the Elaeagnus chinensis seeds at 18°C ​​for 48 hours. The hydration treatment solution required for hydration treatment was prepared by dissolving 23 ml of potassium iodide, 11 ml of p-hydroxybenzoic acid, 8 ml of oxalic acid and 16 ml of malic acid in 220 ml of anhydrous acetone. The hydration treatment solution was heated to 40°C and the low-activity seeds obtained by wet balance treatment were treated for 40 minutes. The low-activity seeds after hydration treatment were quickly dried to a moisture content of 13.09 g by convection using a 65°C hot air flow. The wet balance treatment-hydration-convection drying treatment was repeated 4 times.

[0062] Comparative Example 3

[0063] The method is basically the same as Example 1, except that the step of improving seed activity in step (2) is different.

[0064] (2) Seed activity treatment: The hydration treatment solution required for the hydration treatment was prepared by dissolving 30 ml of cysteine, 23 ml of potassium iodide, 11 ml of p-hydroxybenzoic acid, 8 ml of oxalic acid, 16 ml of malic acid, and 64 ml of cross-linked sodium polyacrylate in 220 ml of anhydrous acetone. The hydration treatment solution was heated to 40°C and treated for 40 min for the low-activity seeds obtained by the moisture balance treatment. The hydrated low-activity seeds were rapidly dried to a moisture content of 13.09 g by convection using a hot air flow at 85°C. The hydration-convection drying treatment was repeated four times.

[0065] Comparative Example 4

[0066] Example 1 is basically the same, except that the high-voltage electrostatic field treatment in step (2) is different.

[0067] (2) Seed activity treatment: Low-activity seeds of Elaeagnus truncatula were placed in an electrostatic field with a corona circle diameter of 0.9 mm. The seeds were treated with a high-voltage direct current of 400 kV / m generated by the electrostatic field for 6 minutes. 152 g of 1 cm thick fallen leaves under the Elaeagnus truncatula forest were mechanically crushed, and 28 g of cellulose hydrolase and 20 g of pectin hydrolase were added to soak and compost, and then filtered to obtain humic acid. The steam liquid for wet balance treatment was made of 200 g of humic acid, 14 g of chitin, and 21 g of sodium silicate. The steam liquid was used to wet balance the Elaeagnus truncatula seeds at 18°C ​​for 48 hours. The hydration treatment solution required for hydration treatment is prepared by dissolving 30 ml of cysteine, 23 ml of potassium iodide, 11 ml of p-hydroxybenzoic acid, 8 ml of oxalic acid, 16 ml of malic acid, and 64 ml of cross-linked sodium polyacrylate in 220 ml of anhydrous acetone. The hydration treatment solution is heated to 40°C and the low-activity seeds obtained by wet balance treatment are treated for 40 minutes. The low-activity seeds after hydration treatment are quickly dried to a moisture content of 13.09 g by convection using a 65°C hot air flow. The wet balance treatment-hydration-convection drying treatment is repeated 4 times.

[0068] Comparative Example 5

[0069] The method is basically the same as that in Example 1, the difference is that the composition of the liquid medicine used for seed activity treatment in step (2) is different.

[0070] (2) Seed activity treatment: Low-activity seeds of Elaeagnus truncatula were placed in an electrostatic field with a corona circle diameter of 0.7 mm, and treated in a high-voltage direct current of 320 kV / m generated by the electrostatic field for 6 min. 152 g of 1 cm thick dead material under the Elaeagnus truncatula forest was mechanically crushed, and 28 g of cellulose hydrolase and 20 g of pectin hydrolase were added to soak and compost, and then filtered to obtain humic acid. The steam liquid for wet balance treatment was made of 200 g of humic acid, 21 g of chitin, and 26 g of sodium silicate. The steam liquid was used to wet balance the Elaeagnus truncatula seeds at 18°C ​​for 48 h. The hydration treatment solution required for hydration treatment is prepared by dissolving 10 ml of cysteine, 13 ml of potassium iodide, 2 ml of p-hydroxybenzoic acid, 1 ml of oxalic acid, 16 ml of malic acid, and 64 ml of cross-linked sodium polyacrylate in 100 ml of anhydrous acetone. The hydration treatment solution is heated to 40°C and the low-activity seeds obtained by wet balance treatment are treated for 40 minutes. The low-activity seeds after hydration treatment are quickly dried to a moisture content of 13.09 g by convection using a 35°C hot air flow. The wet balance treatment-hydration-convection drying treatment is repeated 4 times.

[0071] After the seed activity treatment was completed, random sampling was performed to detect the changes in soluble sugar, lipid, protein content, exudation and conductivity of Examples 1-6 and Comparative Examples 1-5. The results are shown in Table 2.

[0072] Table 2 Detection of soluble sugar, lipid, protein content, extravasation and conductivity changes

[0073]

[0074] As shown in Table 2, using the method of the present invention for improving the activity of Lucerne chinensis seeds by treating low-activity Lucerne chinensis seeds, compared with Comparative Examples 1-5, the contents of soluble sugar, lipid and protein in the obtained seeds were significantly higher than those in the Comparative Examples, and the exosmosis and conductivity of each component were lower than those in the Comparative Examples, indicating that the treatment method of the present invention causes changes in the internal structure and metabolic process of the seeds, changes in the integrity and metabolic process of the membrane, and partially repairs the integrity and damage of the membrane.

[0075] 100 seeds of the southern yew obtained by the treatment of Examples 1-6 and Comparative Examples 1-5 were taken for germination test respectively, and the test was repeated in three groups to observe and record the germination of the southern yew and its vegetative growth after one year. The results are shown in Table 2 below.

[0076] Table 3 Seed germination and vegetative growth

[0077]

[0078] As shown in Table 3, the method of the present invention for improving the activity of the seeds of the southern Chinese cypress by treating the seeds of the southern Chinese cypress with low activity, compared with Comparative Examples 1-5, the germination rate of the seeds of the southern Chinese cypress is relatively high, and the germination time is shortened, which has a slight impact on its subsequent vegetative growth. This shows that the method of the present invention for improving the activity of the seeds can effectively promote the germination of the seeds of the southern Chinese cypress.

[0079] It should be noted that the foregoing description of specific exemplary embodiments of the present invention is for the purpose of illustration and illustration. These descriptions are not intended to limit the present invention to the precise form disclosed, and it is clear that many changes and variations can be made based on the above teachings. The purpose of selecting and describing exemplary embodiments is to explain the specific principles of the present invention and its practical application, so that those skilled in the art can realize and utilize various different exemplary embodiments of the present invention and various different selections and changes. The scope of the present invention is intended to be limited by the claims and their equivalents.

Claims

1. A method for improving the activity of seeds of Lutsea cubeba and promoting their germination, characterized in that: The method comprises the following steps: S1, seed activity detection: explore the correlation between the content of soluble sugar, lipid and protein in the seeds of southern Chinese cypress and seed activity, establish a linear regression formula between the content of soluble sugar, lipid and protein and seed activity, substitute the content of soluble sugar, lipid and protein into the linear regression formula to obtain the seed activity of southern Chinese cypress. When the number of seeds with low activity of southern Chinese cypress in the sample accounts for ≥60% of the total number of samples, all seeds are treated to improve seed activity, among which, The linear regression formula of the content of soluble sugar, lipid, protein and seed activity is: seed activity = 16.945 + 0.912*soluble sugar + 1.390*lipid + 1.334*protein, The low-activity seeds of the southern Chinese cypress refer to seeds of the southern Chinese cypress whose activity is less than 60 obtained by substituting the contents of soluble sugar, lipid and protein obtained by testing into the linear regression formula; S2, seed activity treatment: treating the low-activity seeds of the Chinese fir with high-voltage direct current electrostatic treatment, and then performing wet balance treatment with steam liquid, hydration treatment with hydration treatment liquid, and convection drying treatment. After multiple cycles of wet balance treatment-hydration treatment-convection drying treatment, high-activity seeds of the Chinese fir are obtained. The steam liquid composition includes humic acid, chitin, and sodium silicate. The hydration treatment liquid is anhydrous acetone dissolved cysteine, potassium iodide, p-hydroxybenzoic acid, oxalic acid, malic acid, and cross-linked sodium polyacrylate. The high-voltage direct current electrostatic treatment is to place the low-activity seeds in an electrostatic field with a corona diameter of 0.4-0.7 mm, and the electrostatic field generates a high-voltage direct current of 150-320 KV / m to treat the low-activity seeds for 6-12 minutes; The steam liquid is made of 150-200g humic acid, 8-14g chitosan, and 17-21g sodium silicate. The humic acid is obtained by mechanically breaking up 1-5cm of dead leaves under the southern Chinese fir forest, adding cellulose hydrolase and pectin hydrolase, soaking and composting according to the ratio of dead leaves: cellulose hydrolase: pectin hydrolase = 76-83:10-14:7-10, and then filtering. The hydration treatment solution uses 150-220 ml of anhydrous acetone to dissolve 20-30 ml of cysteine, 18-23 ml of potassium iodide, 6-11 ml of p-hydroxybenzoic acid, 4-8 ml of oxalic acid, 12-16 ml of malic acid, and 50-64 ml of cross-linked sodium polyacrylate.

2. The method for improving the activity of seeds of Lucerne chinensis and promoting their germination according to claim 1, characterized in that: The wet balance treatment described in S2 is to treat the low-activity seeds after high-voltage direct current electrostatic treatment with steam liquid at a temperature of 18-24°C for 24-48 hours.

3. The method for improving the activity of seeds of Lucerne chinensis and promoting their germination according to claim 1, characterized in that: S2 The hydration treatment method is to heat the hydration treatment liquid to a temperature of 38-45°C and treat the low-activity seeds after the wet balance treatment for 30-50 minutes.

4. The method for improving the activity of seeds of Lutsea cubeba and promoting their germination according to claim 1, characterized in that: The convection drying method described in S2 is to use a 40-60° C. hot air flow to quickly dry the low-activity seeds after hydration treatment in a convection manner.

5. The method for improving the activity of seeds of Lutsea cubeba and promoting their germination according to claim 1, characterized in that: The multiple cycles described in S2 refer to 2-4 cycles.

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