A composition containing regenerative medicine material and its preparation method and application

Abstract

The present invention discloses a composition containing regenerative medicine materials, a preparation method and application thereof, wherein the active ingredients of the composition include regenerative medicine materials and Centella asiatica extract, and the weight ratio of the regenerative medicine materials to the Centella asiatica extract is (1-10):1. The preparation method first places the Centella asiatica extract and the regenerative medicine material in a solvent, mixes them evenly, and then dries to volatilize the solvent to obtain the composition, wherein the regenerative medicine material is a regenerative silicon material with a porous structure; the Centella asiatica extract is partially or completely located in the pores of the regenerative silicon material. The composition has a synergistic effect, and has better skin moisturizing effect, sensitive skin repair effect and anti-inflammatory effect.

Description

Technical Field

[0001] The invention relates to the field of skin care, and in particular to a composition containing a regenerative medicine material, a preparation method and an application thereof. Background Art

[0002] Skin is the largest organ in the human body. The tissue on the surface of the body that wraps around the muscles is called skin. The skin is composed of the epidermis, dermis and subcutaneous tissue. The skin is classified into dry skin, neutral skin, oily skin, mixed skin and sensitive skin. Sensitive skin is not a disease, but a sub-healthy state of the skin. Sensitive skin is divided into congenital and acquired. The root cause of acquired sensitive skin is that the skin cells are damaged, which reduces the skin's immunity, and the stratum corneum becomes thinner, resulting in insufficient skin moisture. Ultimately, the skin barrier function is reduced and cannot resist external stimulation of the skin. After being stimulated by the outside world, the skin is prone to hyperactivity, resulting in redness, fever, itching, tingling and other discomforts. Due to the increase in the number of people with sensitive skin in recent years, consumers are also paying more and more attention to sensitive skin and skin-related problems caused by skin sensitivity, making this field a hot spot for research in the cosmetics industry.

[0003] The cause of sensitive skin is not a single factor, and there is uncertainty. The main possible factors that lead to allergic symptoms are as follows: 1) Damage to the skin barrier function. Due to the weakness of the epidermal stratum corneum, the skin barrier function is reduced, and external environmental allergens are more likely to enter, resulting in inflammatory reactions in the skin. 2) Frequent inflammatory reactions lead to further damage to the skin barrier. 3) Overreaction of the subcutaneous nervous system. 4) The living environment is prone to contact with allergens, resulting in damage to the skin's protective layer.

[0004] People with sensitive skin are prone to a variety of skin problems. Acne is a common skin problem. Acne is also called pimples and blackheads. It is a chronic inflammatory disease of the hair follicles and sebaceous glands caused by the combined effects of multiple factors. It is closely related to factors such as bacterial infection, inflammatory response, incomplete cleaning leading to hair follicle blockage and excessive sebum secretion. It is easy to relapse. In severe cases, pigment red marks or concave scars are left after healing. 80% of the population will be troubled by acne at some stage in their lives, especially for teenagers. The psychological and social impact is great. Therefore, acne removal has always been one of the most important research topics in cosmetics.

[0005] To care for sensitive skin, some natural, non-irritating skin care products are needed. Many manufacturers will add some plant extracts with anti-allergic and anti-inflammatory functions to care for sensitive skin and reduce the irritation of the product to the skin. However, not all plant extracts can show the efficacy of the plants themselves. Adding compound plant extracts to products may not achieve the expected results, and may even result in mutual inhibition of efficacy. Effectively exerting the efficacy of plant extracts is an issue that sensitive skin care products need to focus on.

[0006] For skin problems such as sensitive skin acne, the acne removal products currently on the market mainly remove acne through the following methods: exfoliation, antibiotics to inhibit bacteria, comprehensive oil control and glucocorticoids to fight inflammation, but all of them have corresponding problems. Using scrubs and other methods to remove dead skin to solve acne problems can easily damage the skin barrier, making the skin sensitive and easily irritated; blindly using antibiotics will destroy the normal microbial flora of the skin, and excessive oil absorption will cause the moisturizing sebum film on the surface of the skin to be damaged, and the skin will become dry; using hormone ingredients can effectively inhibit inflammatory reactions, but long-term use will produce dependence. The above methods can only remove acne, but have no repair effect on broken sores, and cannot further repair damaged skin. The skin needs to repair itself, so it is of great significance to find a product that can repair damaged skin after acne.

[0007] Centella asiatica (L.) Urban, scientific name, alias: copper coin grass, horse hoof grass, money tooth grass, collapsing bowl, etc., was first recorded in "Shennong's Herbal Classic". "Compendium of Materia Medica" records that the whole plant is used as medicine, with sweet, spicy and cool nature. Its main functions are used for pain relief, blood circulation and blood stasis, skin growth promotion, summer diarrhea, dysentery, refreshing the brain, etc. With the development of medicinal plants, Centella asiatica has gradually been used in cosmetics. Modern science has found that Centella asiatica has the effects of promoting wound healing and inhibiting skin scar formation. However, the characteristics of asiaticoside and other components in Centella asiatica extracts, such as large molecular weight, low water solubility and poor lipophilicity, are not conducive to their diffusion through the stratum corneum, limiting the efficacy and application of Centella asiatica extracts. At the same time, after using Centella asiatica extracts on the skin, it may cause local skin itching, pain, burning, irritation and other side effects. Therefore, how to effectively exert the efficacy of Centella asiatica extracts while reducing its local irritation side effects is of great significance to the application of Centella asiatica in cosmetics. Summary of the invention

[0008] In view of the above-mentioned defects of the prior art, the present invention provides a composition containing regenerative medicine materials with simple ingredients and a preparation method and application thereof. The composition can improve the efficacy of plant extracts, reduce the irritation of the extracts to the skin, and accelerate the recovery of the skin barrier after acne. The composition has the effects of repairing sensitive skin, removing acne, and restoring the skin barrier.

[0009] In order to achieve the technical effect, the technical solution adopted by the present invention is as follows:

[0010] In a first aspect, the present invention provides a composition containing a regenerative medicine material, wherein the active ingredients of the composition include a regenerative medicine material and a Centella asiatica extract, wherein the weight ratio of the regenerative medicine material to the Centella asiatica extract is (1-10):1 (for example: 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1); the regenerative medicine material is a regenerative silicon material having a porous structure; and the Centella asiatica extract is partially or completely located in the pores of the regenerative silicon material.

[0011] In the above composition, as a preferred embodiment, the regenerative medicine material has a porous structure with a specific surface area of ​​200 to 350 m 2 / g(for example: 205m 2 / g, 210m 2 / g, 230m 2 / g, 250m 2 / g, 270m 2 / g, 290m 2 / g, 310m 2 / g, 330m 2 / g, 340m 2 / g, 345m 2 / g).

[0012] In the above composition, as a preferred embodiment, the composition of the regenerative medicine material is expressed by the molar percentage of SiO2, P2O5, and CaO, with a SiO2 content of 28.5-81% (for example: 28.5%, 30%, 40%, 50%, 60%, 70%, 80%, 81%), a P2O5 content of 2.7-14.0% (for example: 2.7%, 3%, 5%, 10%, 12%, 14%), and a CaO content of 16.2-57.5% (for example: 17%, 20%, 25%, 28.5%, 30%, 40%, 50%, 60%, 70%, 80%, 81%).

[0013] In the above composition, as a preferred embodiment, the particle size range of the regenerative medicine material is 50nm-50μm (for example: 60nm, 80nm, 100nm, 200nm, 500nm, 1μm, 10μm, 20μm, 30μm, 40μm, 45μm, 48μm).

[0014] In the above composition, as a preferred embodiment, the regenerative medicine material is RegeSi regenerated silicon.

[0015] The RegeSi regenerated silicon mentioned in the present invention is prepared according to the method in the Chinese patent application publication number: CN111017934A. The above-mentioned RegeSi regenerated silicon includes: a porous structure scaffold formed by the accumulation of nano-scale silicon dioxide particles, and calcium and phosphorus elements uniformly distributed in the scaffold, which is a biologically active calcium phosphosilicate compound; the specific surface area of ​​the regenerated silicon is 200-350m 2 / g; the corresponding commercially available product is regenerated silicon produced by Xingfu Yisheng Regenerative Medicine Technology Co., Ltd.

[0016] In the above composition, as a preferred embodiment, the composition is obtained by the following preparation method: firstly, the Centella asiatica extract and the regenerative medicine material are placed in a solvent, mixed evenly and then dried to volatilize the solvent, thereby obtaining the composition.

[0017] In the above composition, as a preferred embodiment, the Centella asiatica extract includes one or more of asiatica glycoside, madecassoside, asiatic acid, and madecassoside.

[0018] In the above composition, as a preferred embodiment, the solvent is water and / or ethanol.

[0019] In the above composition, as a preferred embodiment, the weight ratio of the regenerative medicine material and the Centella asiatica extract is (1-5):1 (for example: 1.2:1, 1.5:1, 2:1, 3:1, 4:1, 4.5:1, 4.8:1), and more preferably 5:1.

[0020] In the above composition, as a preferred embodiment, the composition is a pharmaceutical composition, a cosmetic, a care product, or a beauty product.

[0021] In the above composition, as a preferred embodiment, the dosage form of the composition includes one or more of lotion, liniment, tincture, oil, ointment, plaster, paste, ironing agent, plaster, film, gel, spray, emulsion, injection, lyophilized powder, aerosol, tablet, and capsule.

[0022] In the above composition, as a preferred embodiment, the composition further contains a pharmaceutically or cosmetically acceptable carrier or excipient.

[0023] In the present invention, when the composition is a cosmetic, skin care product or beauty product, it can be a cream, lotion, paste, ointment, mask, gel or lotion, etc. Cosmetic, skin care product or beauty product bases, auxiliary materials, carriers, additives, etc. known in the prior art can be used, mixed according to conventional methods, and prepared into skin care water, essence, mask, stock solution, ampoule, lotion, face cream, eye cream, etc.

[0024] In a second aspect, the present invention provides a method for preparing the composition described in the first aspect, the method comprising: first placing the Centella asiatica extract and the regenerative medicine material in a solvent, mixing them evenly and then drying them to volatilize the solvent to obtain the composition.

[0025] In the above preparation method, as a preferred embodiment, the method comprises the following steps in sequence:

[0026] (1) dissolving the Centella asiatica extract in a solvent to prepare a Centella asiatica solution;

[0027] (2) dispersing the regenerative medicine material into the Centella asiatica solution and mixing evenly to obtain a mixed solution;

[0028] (3) Drying the mixed solution to obtain the composition.

[0029] By adopting the above method, the Centella asiatica extract is first dissolved in a solvent and evenly dispersed in the solvent to increase the fluidity of the Centella asiatica extract. The regenerative medicine material is then added to evenly disperse the Centella asiatica extract in the solution containing the Centella asiatica extract. The Centella asiatica extract with increased fluidity can enter the porous structure of the regenerative medicine material faster, more and better, so that part or all of the Centella asiatica extract is located in the pores of the regenerative medicine material, which is beneficial to fully exerting the carrying function of the regenerative medicine material, increasing the carrying capacity of the Centella asiatica extract in the regenerative medicine material, and finally obtaining a composition with a high content of Centella asiatica extract.

[0030] In the above preparation method, as a preferred embodiment, the solvent is water and / or ethanol.

[0031] In the above preparation method, as a preferred embodiment, the regenerative medicine material is dispersed into the Centella asiatica solution by ultrasonic treatment and then mixed evenly.

[0032] Ultrasonic mixing is used to increase the movement of active ingredients of extracts such as Centella asiatica glycoside, which is conducive to more active ingredients of the extract entering the porous structure of the regenerative medicine material. However, simple physical mixing, which relies only on dynamics, will result in relatively less active ingredients of Centella asiatica (i.e., Centella asiatica extract) entering the pores of the regenerative medicine material.

[0033] In the above preparation method, as a preferred embodiment, the frequency of the ultrasonic treatment is 10 to 50 kHz (for example: 12 kHz, 15 kHz, 20 kHz, 30 kHz, 40 kHz, 45 kHz, 48 kHz), and more preferably 20 kHz. When the ultrasonic frequency is lower than 10 kHz, it is not conducive to the entry of active ingredients such as Centella asiatica extract into the porous structure of the regenerative medicine material, resulting in a small amount of active substance loading in the composition. When the ultrasonic frequency is higher than 50 kHz, the high frequency destroys the porous structure of the regenerative medicine material, resulting in fewer pores and less active substance loading.

[0034] In the above preparation method, as a preferred embodiment, the ultrasonic treatment time is 2 to 20 minutes (for example: 3 minutes, 5 minutes, 10 minutes, 15 minutes, 18 minutes), and more preferably 10 minutes.

[0035] In the above preparation method, as a preferred embodiment, the mass ratio of the Centella asiatica extract to the solvent is 1:(18-22) (for example: 1:19, 1:20, 1:21), and more preferably 1:20.

[0036] In a third aspect, the present invention provides the use of the composition described in the first aspect or the composition obtained by the preparation method described in the second aspect in the preparation of cosmetics, skin care products, beauty products, or medicines for repairing sensitive skin and / or removing acne and / or repairing the skin barrier.

[0037] In a fourth aspect, the application of regenerative medicine materials in the preparation of cosmetics, skin care products, beauty products, or medicines containing Centella asiatica extract, especially madecassoside; the regenerative medicine material is RegeSi regenerated silicon, and the cosmetics, skin care products or beauty products have at least one of the effects of non-allergenicity, moisturizing, repairing sensitive skin, and anti-inflammatory.

[0038] The inventors unexpectedly discovered that RegeSi regenerated silicon and Centella asiatica extract, especially madecassoside, can reduce the allergenicity of madecassoside when mixed using an appropriate method, and that RegeSi regenerated silicon and madecassoside mixed in a specific proportion can work synergistically to achieve better skin moisturizing effects, sensitive skin repair effects, and anti-inflammatory effects.

[0039] Compared with the prior art, the present invention has at least the following beneficial effects:

[0040] 1. The present invention provides a regenerative medicine material composition, which has the functions of repairing sensitive skin, removing acne, and restoring skin barrier. The composition adopts a compound of regenerative medicine materials and Centella asiatica extract, which greatly reduces the side effects of Centella asiatica extract on the skin, such as itching, pain, burning sensation, irritation, etc.;

[0041] 2. The present invention provides a method for preparing a regenerative medicine material composition. The composition obtained by the preparation method exhibits a synergistic effect of the regenerative medicine material and the Centella asiatica extract, and its efficacy in removing acne and restoring the skin barrier is significantly enhanced;

[0042] 3. The composition provided by the present invention has a simple formula and does not contain additional active ingredients of Western medicine. Since the cost of Centella asiatica extract is relatively high, the material utilization rate of Centella asiatica extract can be fully improved and its usage can be reduced, thereby effectively reducing the cost. In the present invention, through the specific ratio of regenerative medicine materials and Centella asiatica glycoside, the transdermal absorption efficiency of Centella asiatica glycoside is promoted through synergistic effect, and its effect is efficiently exerted, thereby effectively reducing the usage of Centella asiatica glycoside and reducing the cost. DETAILED DESCRIPTION

[0043] In order to make the purpose, technical scheme and advantages of the present invention clearer, the technical scheme in the embodiment of the present invention will be clearly and completely described in conjunction with the embodiments of the present invention. It should be understood by those skilled in the art that the embodiments are only to help understand the present invention and should not be regarded as specific limitations of the present invention. Based on the embodiments in the present invention, all other embodiments obtained by ordinary technicians in the field without making creative work are within the scope of protection of the present invention.

[0044] The embodiments of the present invention are implemented on the premise of the technical solution of the present invention, and detailed implementation methods and processes are given, but the protection scope of the present invention is not limited to the following embodiments. The process parameters of the following embodiments that do not specify specific conditions are usually based on conventional conditions.

[0045] The endpoints and any values ​​of the ranges disclosed in the present invention are not limited to the precise ranges or values, and these ranges or values ​​should be understood to include values ​​close to these ranges or values. For numerical ranges, the endpoint values ​​of each range, the endpoint values ​​of each range and the individual point values, and the individual point values ​​can be combined with each other to obtain one or more new numerical ranges, which should be regarded as specifically disclosed in the present invention.

[0046] In the present invention, unless otherwise specified and / or described, all numerical values ​​involving the amounts of components are "parts by weight" from beginning to end. The process parameters in the following examples without specifying specific conditions are usually based on conventional conditions.

[0047] If no specific techniques or conditions are specified in the examples, the techniques or conditions described in the literature in the field or the product instructions are used. If no manufacturer is specified for the reagents or instruments used, they are all conventional products that can be purchased through regular channels.

[0048] The Centella asiatica extracts listed in the present invention, including asiaticoside, madecassoside, asiatic acid, and madecassoside, all have the characteristics of large molecular weight, low water solubility, and poor lipophilicity, which are not conducive to diffusion through the stratum corneum, and may cause side effects such as itching, pain, burning, and irritation of the local skin when used alone on the skin. Therefore, the composition or composition preparation method of the present invention is suitable for the preparation of a composition containing these Centella asiatica extracts. The following examples are mainly described by taking madecassoside as an example.

[0049] The regenerative medicine materials used in the following examples and comparative examples are all RegeSi regenerated silicon, which is a commercially available product of Beijing Xingfu Yisheng High-tech Co., Ltd. The regenerated silicon product has a porous structure scaffold formed by the accumulation of nano-scale silica particles, and calcium and phosphorus elements uniformly distributed in the scaffold, and is a biologically active calcium phosphosilicate compound.

[0050] Example 1

[0051] This embodiment provides a method for preparing a composition containing a regenerative medicine material, and the specific steps are as follows:

[0052] In parts by weight, 1 part of madecassoside was added to 20 parts of water. After dissolution, 5 parts of RegeSi regenerated silicon were added, ultrasonically treated (20 kHz, 10 min), and dried in an oven at 50° C. to obtain a composition.

[0053] Example 2

[0054] This embodiment provides a method for preparing a composition containing a regenerative medicine material, and the specific steps are as follows:

[0055] In parts by weight, 1 part of madecassoside was added to 20 parts of water. After dissolution, 5 parts of RegeSi regenerated silicon were added, ultrasonic treatment was performed (10 kHz, 10 min), and drying was carried out in an oven at 50° C. to obtain a composition.

[0056] Example 3

[0057] This embodiment provides a method for preparing a composition containing a regenerative medicine material, and the specific steps are as follows:

[0058] In parts by weight, 1 part of madecassoside was added to 20 parts of water. After dissolution, 5 parts of RegeSi regenerated silicon were added, ultrasonically treated (50 kHz, 10 min), and dried in an oven at 50° C. to obtain a composition.

[0059] Example 4

[0060] This embodiment provides a method for preparing a composition containing a regenerative medicine material, and the specific steps are as follows:

[0061] In parts by weight, 1 part of madecassoside was added to 20 parts of water. After dissolution, 1 part of RegeSi was added, and the mixture was ultrasonically treated (20 kHz, 10 min) and dried in an oven at 50° C. to obtain a composition.

[0062] Example 5

[0063] This embodiment provides a method for preparing a composition containing a regenerative medicine material, and the specific steps are as follows:

[0064] In parts by weight, 1 part of madecassoside was added to 20 parts of water. After dissolution, 10 parts of RegeSi regenerated silicon were added, ultrasonically treated (20 kHz, 10 min), and dried in an oven at 50° C. to obtain a composition.

[0065] Example 6

[0066] This embodiment provides an essence milk, and its composition is as follows (mass percentage): 2% of the composition of embodiment 1, and 98% of the essence milk base.

[0067] The composition of the essence milk matrix is ​​as follows (mass percentage):

[0068] Xanthan gum 0.3%, glycerin 3%, potassium lauryl phosphate 1.5%, polydimethylsiloxane 3%, jojoba (SIMMONDSIA CHINENSIS) seed oil 5%, avocado (BUTYROSPERMUM PARKII) fruit butter 2%; polyglyceryl-6 distearate 3%, cetearyl alcohol 2%, mandelic acid 2%, deionized water 78.2%.

[0069] Example 7

[0070] This embodiment provides an essence milk, and its composition is as follows (mass percentage): 2% of the composition of Example 2, and 98% of the essence milk base.

[0071] The essence milk base formula is the same as that in Example 6.

[0072] Example 8

[0073] This embodiment provides an essence milk, and its composition is as follows (mass percentage): 2% of the composition of Example 3 and 98% of the essence milk base.

[0074] The essence milk base formula is the same as that in Example 6.

[0075] Example 9

[0076] This embodiment provides an essence milk, and its composition is as follows (mass percentage): 2% of the composition of Example 4 and 98% of the essence milk base.

[0077] The essence milk base formula is the same as that in Example 6.

[0078] Example 10

[0079] This embodiment provides an essence milk, and its composition is as follows (mass percentage): 2% of the composition of Example 5 and 98% of the essence milk base.

[0080] The essence milk base formula is the same as that in Example 6.

[0081] Embodiment 11

[0082] This embodiment provides a method for preparing a composition containing a regenerative medicine material, and the specific steps are as follows:

[0083] In parts by weight, 1 part of madecassoside and 5 parts of RegeSi were added to 20 parts of water, stirred until uniformly dispersed in the water, and dried in an oven at 50° C. to obtain a composition.

[0084] Example 12

[0085] This embodiment provides a method for preparing a composition containing a regenerative medicine material, and the specific steps are as follows:

[0086] In parts by weight, 1 part of madecassoside and 1 part of RegeSi were added to 20 parts of water, stirred until uniformly dispersed in the water, and dried in an oven at 50° C. to obtain a composition.

[0087] Embodiment 13

[0088] This embodiment provides an essence milk, and its composition is as follows (mass percentage): 2% of the composition of Example 11 and 98% of the essence milk base.

[0089] The essence milk base formula is the same as that in Example 6.

[0090] Embodiment 14

[0091] This embodiment provides an essence milk, and its composition is as follows (mass percentage): 2% of the composition of Example 12, and 98% of the essence milk base.

[0092] The essence milk base formula is the same as that in Example 6.

[0093] Comparative Example 1

[0094] This comparative example provides a method for preparing a composition, and the specific steps are as follows:

[0095] In parts by weight, 1 part of madecassoside was added to 20 parts of water, dissolved, ultrasonically treated (20 kHz, 10 min), and dried in an oven at 50° C. to obtain powder.

[0096] Comparative Example 2

[0097] This comparative example provides a method for preparing a composition, and the specific steps are as follows:

[0098] In parts by weight, 5 parts of RegeSi regenerated silicon were added to 20 parts of water, ultrasonically treated (20 kHz, 10 min), and dried in an oven at 50° C. to obtain powder.

[0099] Comparative Example 3

[0100] This comparative example provides a method for preparing a composition, and the specific steps are as follows:

[0101] In parts by weight, 1 part of madecassoside and 5 parts of RegeSi regenerated silicon are mixed and uniformly mixed to obtain a composition.

[0102] Comparative Example 4

[0103] This comparative example provides an essence milk, and its composition is as follows (mass percentage): 0.33% of the composition of comparative example 1 and 99.67% of the essence milk matrix.

[0104] The essence milk base formula is the same as that in Example 6.

[0105] Comparative Example 5

[0106] This comparative example provides an essence milk, and its composition is as follows (mass percentage): 1.67% of the composition of comparative example 2, and 98.33% of the essence milk matrix.

[0107] The essence milk base formula is the same as that in Example 6.

[0108] Comparative Example 6

[0109] This comparative example provides an essence milk, and its composition is as follows (mass percentage): 2% of the composition of comparative example 3 and 98% of the essence milk base.

[0110] The essence milk base formula is the same as that in Example 6.

[0111] Comparative Example 7

[0112] This comparative example provides an essence milk, which is composed of 100% essence milk base.

[0113] The essence milk base formula is the same as that in Example 6.

[0114] Test Example 1: Sensitization Effect Test

[0115] 100 female guinea pigs were randomly divided into 10 groups according to their weight levels, with 10 guinea pigs in each group, and the compositions prepared in Examples 1-5, Example 11, Example 12 and Comparative Examples 1-3 were applied respectively for sensitization test. The application doses were: Example 1 (2 g), Example 2 (2 g), Example 3 (2 g), Example 4 (2 g), Example 5 (2 g), Example 11 (2 g), Example 12 (2 g), Comparative Example 1 (0.33 g), Comparative Example 2 (1.67 g), Comparative Example 3 (2 g).

[0116] Induction period: About 24 hours before the test, the left side of the guinea pig's back was depilated, and the depilated area was 6cm×6cm. 2% salicylic acid was applied to the depilated area of ​​4cm×4cm on the back of the guinea pig. After the salicylic acid dried, the compositions prepared in Examples 1-5, Example 11, Example 12 and Comparative Examples 1-3 were applied to the depilated area on the back of the guinea pig for administration. After sealing and fixing with non-irritating adhesive tape for 6 hours, the covering was removed and the residual test sample was cleaned. The administration was carried out three times in total, specifically on the 1st, 8th and 15th days. Before each administration, the corresponding position of the administration area of ​​each group of animals was depilated again.

[0117] Provocation period: About 24 hours before the test, the left side of the guinea pig's back was depilated, and the depilated area was 6cm×6cm. 2% salicylic acid was applied to the depilated area of ​​4cm×4cm on the back of the guinea pig. After the salicylic acid dried, the compositions prepared in Examples 1-5, Example 11, Example 12 and Comparative Examples 1-3 were applied to the depilated area on the back of the guinea pig for administration. After sealing and fixing with non-irritating adhesive tape for 6 hours, the covering was removed and the residual test sample was cleaned. The administration was carried out three times in total, specifically on the 1st, 8th and 15th days. Before each administration, the corresponding position of the administration area of ​​each group of animals was depilated again.

[0118] The skin reaction of guinea pigs was observed 24h and 48h after administration during the provocation period. The skin allergic reaction of each animal was scored according to the skin allergic reaction scoring standard (Table 1). The skin reaction score results obtained by averaging the data of each group are shown in Table 2 below.

[0119] Table 1 Scoring criteria for skin allergic reactions

[0120] Patch test reaction Scoring Criteria No significant changes 0 Scattered or macular erythema 1 Moderate confluent erythema 2 Severe erythema and / or edema 3

[0121] Table 2 Results of skin allergic reaction scoring

[0122]

[0123]

[0124] It can be seen from the test results in Table 2 that the skin allergic reaction using the compositions prepared in Examples 1-5, 11, and 12 is significantly lower than that in Comparative Examples 1 and 3, indicating that the compounding of RegeSi regenerated silicon and madecassoside in the compositions prepared in Examples 1-5, 11, and 12 can effectively reduce the irritation of madecassoside (Comparative Example 1 is madecassoside alone);

[0125] Compared with Comparative Example 3 (directly mixing madecassoside and RegeSi regenerated silicon), the composition preparation process in Examples 1-5, 11, and 12 can reduce the irritation of the composition. Through the preparation process of the present invention, the irritation of madecassoside to the skin can be effectively reduced, thereby expanding the application scope of madecassoside in the field of cosmetics.

[0126] Test Example 2 Analysis of Skin Nourishing and Moisturizing Effects

[0127] The skin nourishing and moisturizing effects of the essence milks prepared in Examples 6-10, Examples 13-14, and Comparative Examples 4-7 were evaluated respectively, and the specific method is as follows:

[0128] 110 volunteers were selected, aged between 25 and 60 years old, including 38 males and 72 females; they were randomly divided into 11 groups, with 10 people in each group. 5×5cm was selected at the skin for the experiment, and the dosage of the essence milk in each group was the same, which was 2g / time. Using the German CK skin moisture tester Corneometer CM825, the essence milk prepared in Examples 6-10, Examples 13-14, and Comparative Examples 4-7 was applied to the skin. The skin moisture content of each subject at different periods was measured using a skin moisture tester under constant temperature and constant humidity (21°C and 40% humidity). The skin moisture content before application was measured as T0-initial value, the skin moisture content 30 minutes after application was measured as T1-30min, and the skin moisture content 8 hours after application was measured as T2-8h. The test results are shown in Table 3.

[0129] Table 3 Skin moisture content detection

[0130]

[0131]

[0132] It can be seen from the test results in Table 3 that the skin water content 30 minutes after use of the essence lotions prepared in Examples 6-10, 13 and 14 is significantly improved compared with that of Comparative Examples 4-7, among which Example 6 (comprising the composition prepared in Example 1) has the best effect, indicating that the composition prepared by the method of the present invention can nourish and moisturize the skin, provide the skin with sufficient moisture, repair the skin barrier, and delay skin aging.

[0133] Test Example 3: Sensitive Skin Repair Effect Test

[0134] The essence lotions prepared in Examples 6-10, 13 and 14 and Comparative Examples 4-7 were tested for their repairing effects on sensitive skin.

[0135] 55 subjects with sensitive skin, aged between 25 and 60 years old, were randomly divided into 11 groups, each with 5 subjects. Each group of subjects used the essence milk prepared by Examples 6-10, 13 and 14 and Comparative Examples 4-7, respectively. The subjects applied the test samples on their faces after cleansing their faces every morning and evening, with the usage amount being 2 g / time. The facial image analyzer VISIA was used to take facial images of the subjects before using the test samples and 4 and 8 weeks after using the test samples, and the scores of the red areas on the face were obtained by analysis. The test results are shown in Table 4.

[0136] Note: Face red discrimination value: take the average value of the test results of each group of subjects;

[0137] Table 4 Skin redness distinction values

[0138]

[0139]

[0140] According to the data in Table 4, compared with Comparative Examples 4-7, the facial redness discrimination values ​​of the essence lotions prepared in Examples 6-9 were significantly improved after 4 and 8 weeks, and the facial redness discrimination values ​​of the essence lotions prepared in Examples 10, 13 and 14 were also above 68% after 8 weeks of use, indicating that the composition of the present invention can repair sensitive skin, among which the essence lotion containing the composition of Example 1 (Example 6) has the best effect.

[0141] Test Example 4 Anti-inflammatory Test

[0142] The anti-inflammatory test was performed on the essence milks prepared in Examples 6-10, 13 and 14, and Comparative Examples 4-7.

[0143] The test reagents and test methods are as follows:

[0144] Reagents: Inflammatories, 100% xylene.

[0145] Test method: The essence milk prepared in Examples 6-10, 13 and 14, and Comparative Examples 4-7 were mixed with an inflammatory agent (100% xylene) at a volume ratio of 1:1. 120 mice were selected and randomly divided into 12 groups, 10 mice in each group, 1 blank control group and 11 sample groups. The inflammatory agent (blank control group) or the inflammatory agent containing the sample (test group) was applied to the front and back surfaces of the right ear of the test mouse. The volume of the inflammatory agent was 0.02 mL / mouse. No treatment was performed on the left ear. After 1 hour, the animal was anesthetized and killed, both ears were cut off, and round ear pieces were punched in the same part of the left and right ear pieces with a 9 mm diameter puncher, and weighed. The swelling degree is the weight of the right ear piece of each mouse minus the weight of the left ear piece. The swelling degree of the sample group and the blank control group is statistically processed and the swelling inhibition rate (%) is calculated. The swelling inhibition rate = (the mean difference of the swelling degree of the blank control group - the mean difference of the swelling degree of the test group) / the mean difference of the swelling degree of the blank control group × 100%. The experimental results are shown in Table 5, wherein the P value is the statistical significance of the difference in the swelling degree of each embodiment and comparative example relative to the swelling degree of the blank control group.

[0146] Table 5 Effects of samples on ear swelling in mice

[0147]

[0148]

[0149] As shown in Table 5, the essence emulsions prepared in Examples 6-10, 13 and 14 can effectively reduce the swelling of the mouse ears and have anti-inflammatory effects, among which Example 6 has the best effect. At the same time, the swelling inhibition rates of Examples 6-10, 13 and 14 are significantly better than those of Comparative Examples 4-7 as a whole, indicating that the composition of the present invention has excellent anti-inflammatory effects.

[0150] Test Example 5: Anti-inflammatory and soothing effect test

[0151] The essence lotions prepared in Examples 6-10, 13 and 14, and Comparative Examples 4-7 were respectively subjected to anti-inflammatory and soothing tests on sensitive skin.

[0152] Experimental method: Divided into 1 blank control group and 11 sample groups, human peripheral blood mononuclear cells (PBMCs) were used for in vitro testing. The purchased human peripheral blood mononuclear cells were added to RPMI-1640 cell culture medium containing 10% fetal bovine serum by volume and inoculated into 6-well cell culture plates. The number of cells per well was required to be 5×10 6Then, the essence milk prepared in Examples 6-10, 13 and 14, and Comparative Examples 4-7 were placed in the cell culture fluid of 11 sample groups, and the amount of essence milk added to each group was the same. Then, the cell culture plate was placed in a constant temperature cell culture incubator at 37°C and 5% CO2 by volume. After the cells were cultured for 24 hours with or without samples, inflammation was induced with 1 μg / ml LPS (lipopolysaccharide), and the IL-6 content was determined by enzyme-linked immunosorbent assay, with the blank group as 100%. The results are shown in Table 6;

[0153] Table 6 Effect of samples on the release of inflammatory factor IL-6

[0154]

[0155]

[0156] As shown in Table 5, the experimental results show that, compared with Comparative Examples 4-7, Examples 6-10, 13 and 14 have a good effect on reducing the release of IL-6, and Example 6 has the best effect, which can reduce the release of IL-6 by 88.5%. The above results show that the composition of the present invention has excellent anti-inflammatory and soothing effects.

[0157] Although the present invention has been described in detail above with general descriptions and specific embodiments, it is obvious to those skilled in the art that some modifications or improvements may be made thereto based on the present invention. Therefore, these modifications or improvements made without departing from the spirit of the present invention all fall within the scope of protection claimed by the present invention.

Claims

1. A composition containing a regenerative medicine material, characterized in that: The active ingredients of the composition include regenerative medicine material and Centella asiatica extract, wherein the weight ratio of the regenerative medicine material to the Centella asiatica extract is (1-10):1; the regenerative medicine material is a regenerative silicon material with a porous structure; the Centella asiatica extract is partially or completely located in the pores of the regenerative silicon material; The Centella asiatica extract is madecassoside; The regenerative medicine material is RegeSi regenerated silicon; The composition is obtained by the following preparation method: firstly, the Centella asiatica extract and the regenerative medicine material are placed in a solvent, mixed evenly, and then dried to volatilize the solvent, thereby obtaining the composition.

2. The composition according to claim 1, characterized in that The weight ratio of the regenerative medicine material to the Centella asiatica extract is (1-5):

1.

3. The composition according to claim 1, characterized in that The weight ratio of the regenerative medicine material to the Centella asiatica extract is 5:

1.

4. The composition according to claim 1, characterized in that The composition is a pharmaceutical composition or a cosmetic; and / or: the dosage form of the composition includes one or more of lotion, liniment, tincture, oil, ointment, plaster, paste, ironing agent, plaster, film, gel, spray, emulsion, injection, lyophilized powder, aerosol, tablet, capsule; And / or: the composition further contains a pharmaceutically or cosmetically acceptable carrier or excipient.

5. The composition according to claim 1, characterized in that When the composition is a cosmetic, the specific dosage form is one or more of a cream, an emulsion, a paste, an ointment, a mask, a gel or a lotion.

6. A method for preparing the composition according to any one of claims 1 to 5, characterized in that: The method comprises: firstly placing the Centella asiatica extract and the regenerative medicine material in a solvent, mixing them evenly, and then drying to volatilize the solvent to obtain the composition.

7. The preparation method according to claim 6, characterized in that: The solvent is water and / or ethanol; And / or: the mass ratio of the Centella asiatica extract to the solvent is 1:(18-22); and / or: dispersing the regenerative medicine material and mixing it uniformly by ultrasonic treatment; The frequency of the ultrasonic treatment is 10 to 50 kHz; The ultrasonic treatment time is 2 to 20 minutes.

8. The preparation method according to claim 7, characterized in that: The mass ratio of the Centella asiatica extract to the solvent is 1:

20.

9. The preparation method according to claim 7, characterized in that: The frequency of the ultrasonic treatment is 20 kHz; the time of the ultrasonic treatment is 10 min.

10. Use of the composition according to any one of claims 1 to 5 or the composition prepared by any one of the methods according to claims 6 to 9 in preparing cosmetics or medicines for repairing sensitive skin and / or removing acne and / or repairing skin barrier.

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