Four-season traditional Chinese medicine tea and preparation method thereof

Abstract

The present application relates to the technical field of medicinal and edible products, in particular to a four-season traditional Chinese medicine tea and a preparation method thereof. The raw materials of the four-season traditional Chinese medicine tea include chrysanthemum, Chinese wolfberry, white peony root extract, marsh marigold, longan aril, lily, embelia laevigata extract, poria cocos, ophiopogon japonicus, orange red, fat sea, acanthopanax extract, astragalus polysaccharide, dried ginger, cyperus rotundus extract, polygonatum, cornus officinalis, ginseng, jujube and licorice polysaccharide. The medicinal tea used in different seasons can play different effects, such as improving immunity, calming the heart and soothing the nerves, drying dampness and reducing phlegm, improving constipation, etc. When used in combination, the climate of four seasons and the changes of human body with four seasons can play a better role in regulating the functions of the body.

Description

Technical Field

[0001] This invention relates to the field of food and medicine homology products, specifically to a traditional Chinese medicine tea for all seasons and its preparation method. Background Technology

[0002] Medicinal tea is a special liquid or solid beverage made by adding food or medicine to tea leaves, possessing certain therapeutic effects. It is also a common application of medicinal and edible herbs. In the general public's perception, medicinal tea containing medicinal and edible herbs can more effectively improve health compared to ordinary tea and flower tea, making it increasingly popular. However, while medicinal and edible herbs are generally safe and reliable, improper consumption can still lead to discomfort due to inappropriate processing methods, formulation, and timing of administration. Furthermore, these herbs are often mild in nature, and the dosage used for tea brewing cannot reach the dosage used in medicine, thus failing to achieve the desired effect. Extracting active ingredients is also challenging because the components of medicinal herbs are usually complex, and the extracted components may not achieve the desired effect. Additionally, many medicinal herbs have unpleasant smells and tastes, which can be unpleasant to the eye. Improving these with flavoring agents, whether natural (sweetened) or chemically synthesized (sugar-free), is detrimental to health. All of the above reasons are detrimental to the promotion of Chinese herbal tea. Summary of the Invention

[0003] To address the aforementioned technical problems in the prior art, this invention provides a seasonal herbal tea and its preparation method. The ingredients of this seasonal herbal tea are rationally formulated, and it has the effects of enhancing immunity, calming the mind and soothing the nerves, relieving dampness and resolving phlegm, and improving constipation.

[0004] To achieve the above-mentioned objectives, the present invention adopts the following technical solution:

[0005] In a first aspect, the present invention provides a seasonal Chinese herbal tea, including spring herbal tea, summer herbal tea, autumn herbal tea, and winter herbal tea, wherein:

[0006] The spring herbal tea comprises the following ingredients in the indicated weight proportions: 2-4 parts chrysanthemum, 1-3 parts wolfberry, 1-3 parts white peony extract, 1-3 parts golden lotus, and 1-3 parts licorice polysaccharide.

[0007] The summer herbal tea comprises the following ingredients in the indicated weight proportions: 2-4 parts longan pulp, 2-4 parts lily bulb, 1-3 parts light bamboo leaf extract, 2-4 parts poria cocos, and 1-3 parts licorice polysaccharide.

[0008] The autumn herbal tea comprises the following ingredients in the indicated weight proportions: 2-4 parts Ophiopogon japonicus, 1-3 parts Citrus reticulata peel, 2-4 parts Sterculia lychnophora, 1-3 parts Acanthopanax senticosus extract, and 1-3 parts Astragalus polysaccharide.

[0009] The winter herbal tea comprises the following ingredients in the indicated weight proportions: 4-6 parts dried ginger, 1-3 parts Cyperus rotundus extract, 5-7 parts Polygonatum sibiricum, 4-6 parts Cornus officinalis, 1-3 parts ginseng, 9-11 parts jujube, and 1-3 parts licorice polysaccharide.

[0010] Among the aforementioned herbal teas for the four seasons:

[0011] Chrysanthemum: bitter and sweet in taste, slightly cold in nature; enters the liver and lung meridians; good at clearing wind-heat evil, calming the liver and improving eyesight, and detoxifying;

[0012] Goji berries: sweet in taste, neutral in nature; enters the liver, kidney, and lung meridians; nourishes yin and assists yang, mainly for liver and kidney yin deficiency;

[0013] White peony root: It is pungent and bitter in taste, and warm in nature; it enters the liver and spleen meridians; it nourishes blood and astringes yin, softens the liver and relieves pain, and calms liver yang.

[0014] Golden lotus: bitter in taste, slightly cold in nature; enters the lung and stomach meridians; clears heat and detoxifies, benefits the liver, and clears the lungs;

[0015] Licorice: sweet in taste and neutral in nature; enters the heart, lung, spleen, and stomach meridians; tonifies the middle qi, clears heat and detoxifies, relieves spasms and pain, and harmonizes the effects of other herbs;

[0016] Longan pulp: sweet in taste and warm in nature; enters the heart and spleen meridians; nourishes the heart and spleen, and benefits qi and blood;

[0017] Lily: Sweet in taste, cold in nature; enters the lung and heart meridians; nourishes yin and moistens the lungs, clears the heart and calms the mind;

[0018] Lophatherum gracile: sweet and bland in taste, cold in nature; enters the heart, spleen, and stomach meridians; clears heat and relieves irritability, nourishes yin and generates fluids;

[0019] Poria cocos: sweet and bland in taste, neutral in nature; enters the heart, spleen, and kidney meridians; promotes diuresis and eliminates dampness, calms the mind, and strengthens the spleen;

[0020] Ophiopogon japonicus: sweet and slightly bitter in taste, slightly cold in nature; enters the lung, heart, and stomach meridians; moistens the lungs and nourishes yin, benefits the stomach and promotes the production of body fluids, clears the heart and relieves irritability;

[0021] Tangerine peel: It has a pungent and bitter taste and is warm in nature; it enters the spleen and lung meridians; it regulates qi, harmonizes the middle jiao, dries dampness, resolves phlegm, and promotes digestion;

[0022] Malva nut: sweet in taste, cold in nature; enters the lung and stomach meridians; clears heat, moistens the lungs, benefits the throat, and detoxifies;

[0023] Acanthopanax senticosus: pungent, slightly bitter, warm; enters the spleen, kidney, and heart meridians; invigorates qi and strengthens the spleen, tonifies the kidneys and calms the mind;

[0024] Astragalus: sweet in taste and slightly warm in nature; enters the spleen and lung meridians; strengthens the spleen and replenishes the middle jiao, raises yang and lifts prolapse, benefits the defensive qi and consolidates the exterior, promotes diuresis, and promotes tissue regeneration and detoxification.

[0025] Dried ginger: pungent in flavor and hot in nature; enters the heart, spleen, stomach, and lung meridians; restores yang and unblocks the meridians, warms the lungs and resolves phlegm, warms the meridians and stops bleeding;

[0026] Cyperus rotundus: It has a pungent, slightly bitter, and slightly sweet taste, and is neutral in nature; it enters the liver, spleen, and triple burner meridians; it soothes the liver, relieves depression, regulates qi, regulates menstruation, and relieves pain;

[0027] Polygonatum: It enters the spleen, lung, and kidney meridians; it moistens the lungs and nourishes yin, replenishes qi and benefits the spleen, and nourishes the kidneys and replenishes essence.

[0028] Cornus officinalis: enters the liver and kidney meridians; tonifies the liver and kidneys, astringes essence and stops sweating, nourishes yin and heart;

[0029] Ginseng: Enters the spleen and lung meridians; greatly replenishes vital energy, nourishes the lungs and spleen, promotes the production of body fluids and quenches thirst, calms the mind and improves intelligence;

[0030] Jujube: It enters the spleen meridian; it benefits the spleen and promotes the production of body fluids, and invigorates the stomach and replenishes qi.

[0031] Spring is the season when liver qi is dominant, making it easy to soothe the liver and clear heat. At the same time, spring is also a high-incidence season for allergies, as environmental allergens can easily trigger various allergic reactions in the body. This invention, through the compatibility of the various ingredients in the above-mentioned spring herbal tea, can regulate immunity and reduce various allergic symptoms caused by excessive reactions to allergens, thereby reducing the incidence or alleviating symptoms of skin allergies, seasonal asthma, etc.

[0032] Summer's high temperatures and abundant rainfall can easily cause irritability and restlessness. High humidity makes it difficult for moisture to dissipate, disrupting the body's temperature regulation and water-electrolyte metabolism. It can also negatively impact the hypothalamus's mood regulation center, leading to emotional instability. Furthermore, people often use air conditioning and cold drinks to cool down in summer, but these methods can cause dampness to accumulate, damaging the spleen and stomach and causing loss of appetite. Cold drinks, usually high in sugar, further burden the spleen and stomach. This invention, through the combination of ingredients in the aforementioned summer herbal tea, can calm the mind and spirit, reduce dampness, and enhance appetite.

[0033] Autumn's dry climate easily leads to dry mouth, throat, and skin. Furthermore, the large temperature difference between day and night and the fluctuating temperatures in autumn make one susceptible to colds. The autumn herbal tea provided by this invention, through the harmonious combination of its ingredients, can dry dampness and resolve phlegm, clear the lungs and soothe the throat, while simultaneously tonifying the spleen and replenishing qi, thus enhancing immunity.

[0034] In winter, low temperatures can easily cause cold hands and feet in people with insufficient Qi and blood, or those experiencing Qi stagnation and blood stasis. This can lead them to spend more time in heated rooms and consume more high-calorie, high-fat foods. This can cause discomfort such as internal heat and constipation, and the large temperature difference between indoors and outdoors can also lead to illness. This invention, through the combination of various ingredients in the aforementioned winter herbal tea, can enhance the body's Yang Qi, promote blood circulation, and improve the symptoms of cold hands and feet. Simultaneously, it can alleviate symptoms of internal heat and constipation caused by dry environments, and enhance the body's vital energy, reducing the risk of illness.

[0035] The above-mentioned seasonal herbal teas can exert certain effects when used alone. When used in combination, they can be combined with the climate of the four seasons and the changes in the human body with the seasons to exert a better effect on regulating bodily functions.

[0036] Preferably, the spring herbal tea comprises the following ingredients in the indicated weight proportions: 2.5-3.5 parts chrysanthemum, 1.5-2.5 parts wolfberry, 1.5-2.5 parts white peony extract, 1.5-2.5 parts golden lotus, and 1.5-2.5 parts licorice polysaccharide.

[0037] Preferably, the spring herbal tea comprises the following ingredients in the indicated weight proportions: 3 parts chrysanthemum, 2 parts wolfberry, 2 parts white peony extract, 2 parts golden lotus, and 2 parts licorice polysaccharide.

[0038] Preferably, the summer herbal tea comprises the following ingredients in the indicated weight proportions: 2.5-3.5 parts longan pulp, 2.5-3.5 parts lily bulb, 1.5-2.5 parts bamboo leaf extract, 2.5-3.5 parts poria cocos, and 1.5-2.5 parts licorice polysaccharide.

[0039] Preferably, the summer herbal tea comprises the following ingredients in the indicated weight proportions: 3 parts longan pulp, 3 parts lily bulb, 2 parts bamboo leaf extract, 3 parts poria cocos, and 2 parts licorice polysaccharide.

[0040] Preferably, the autumn herbal tea comprises the following ingredients in the indicated weight proportions: 2.5-3.5 parts of Ophiopogon japonicus, 1.5-2.5 parts of Citrus reticulata peel, 2.5-3.5 parts of Sterculia lychnophora, 1.5-2.5 parts of Acanthopanax senticosus extract, and 1.5-2.5 parts of Astragalus polysaccharide.

[0041] Preferably, the autumn herbal tea comprises the following ingredients in the indicated weight proportions: 3 parts Ophiopogon japonicus, 2 parts Citrus reticulata peel, 3 parts Sterculia lychnophora, 2 parts Acanthopanax senticosus extract, and 2 parts Astragalus polysaccharide.

[0042] Preferably, the winter medicinal tea comprises the following ingredients in the indicated weight proportions: 4.5-5.5 parts dried ginger, 1.5-2.5 parts Cyperus rotundus extract, 5.5-6.5 parts Polygonatum sibiricum, 4.5-5.5 parts Cornus officinalis, 1.5-2.5 parts ginseng, 9.5-10.5 parts jujube, and 1.5-2.5 parts licorice polysaccharide.

[0043] Preferably, the winter herbal tea comprises the following ingredients in the indicated weight proportions: 5 parts dried ginger, 2 parts Cyperus rotundus extract, 6 parts Polygonatum sibiricum, 5 parts Cornus officinalis, 2 parts ginseng, 10 parts jujube, and 2 parts licorice polysaccharide.

[0044] In conjunction with the first aspect, the preparation method of the white peony extract in the spring medicinal tea is as follows: white peony is ultrasonically extracted with ethanol for 0.5 to 1 hour, followed by solid-liquid separation to obtain ethanol-extracted white peony residue. The obtained residue is then refluxed with 0.1 to 0.3 mol / L sodium carbonate aqueous solution for extraction. The obtained extract is then concentrated and dried to obtain the white peony extract.

[0045] Preferably, the reflux extraction is performed 2 to 3 times, each time for 30 to 45 minutes. After extraction, the mixture is filtered, and the filtrates are combined to obtain the above-mentioned extract.

[0046] In conjunction with the first aspect, the preparation method of the light bamboo leaf extract in the summer medicinal tea is as follows: light bamboo leaves are extracted by reflux with 0.05-0.15 mol / L hydrochloric acid aqueous solution, and the resulting extract is concentrated and dried to obtain the light bamboo leaf extract.

[0047] Preferably, the reflux extraction is performed 2 to 3 times, each time for 30 to 45 minutes. After extraction, the mixture is filtered, and the filtrates are combined to obtain the above-mentioned extract.

[0048] In conjunction with the first aspect, the preparation method of the Acanthopanax senticosus extract in the autumn medicinal tea is as follows: Acanthopanax senticosus is extracted by reflux with 0.05-0.15 mol / L hydrochloric acid aqueous solution, the resulting extract is concentrated and dried to obtain Acanthopanax senticosus aqueous extract and Acanthopanax senticosus residue; the Acanthopanax senticosus residue is extracted by reflux with 75%-85% v / v ethanol, the resulting extract is concentrated and dried to obtain Acanthopanax senticosus alcoholic extract; the Acanthopanax senticosus aqueous extract and Acanthopanax senticosus alcoholic extract are mixed to obtain the Acanthopanax senticosus extract.

[0049] Preferably, the reflux extraction is performed 2 to 3 times, each time for 30 to 45 minutes. After extraction, the mixture is filtered, and the filtrates are combined to obtain the above-mentioned extract.

[0050] In conjunction with the first aspect, the preparation method of the Astragalus polysaccharide in the autumn medicinal tea is as follows: Astragalus is extracted by reflux with water, the resulting extract is concentrated, ethanol is added to the concentration of ethanol to 55% to 65% v / v, the supernatant is separated after standing and precipitation, and the resulting supernatant is concentrated and dried to obtain the Astragalus polysaccharide.

[0051] Preferably, the reflux extraction is performed 3 to 5 times, each time for 60 to 90 minutes. After extraction, the mixture is filtered, and the filtrates are combined to obtain the above-mentioned extract.

[0052] In conjunction with the first aspect, the preparation method of the Cyperus rotundus extract in the winter medicinal tea is as follows: Cyperus rotundus is extracted with 85% to 100% v / v ethanol by ultrasonic extraction, and the resulting extract is concentrated and dried to obtain the Cyperus rotundus extract.

[0053] Preferably, the ultrasonic extraction power is 600-800W, the extraction temperature is 40-60℃, the extraction is performed 2-3 times, and each extraction lasts 60-90 minutes. After extraction, the mixture is filtered, and the filtrates are combined to obtain the above-mentioned extract.

[0054] In conjunction with the first aspect, the preparation method of the licorice polysaccharide is as follows: licorice is extracted with water by ultrasonic extraction to obtain ultrasonic extract and licorice residue; the obtained licorice residue is then extracted with water by reflux to obtain reflux extract; the ultrasonic extract and reflux extract are combined, concentrated, and ethanol is added to an ethanol concentration of 75% to 85% v / v. After standing and precipitation, the supernatant is separated, and the obtained supernatant is concentrated and dried to obtain the licorice polysaccharide.

[0055] Preferably, the ultrasonic extraction power is 600-800W, the extraction temperature is 50-60℃, the extraction is performed 3-4 times, each time for 60-90 minutes, and the filtrates are combined after each extraction; the reflux extraction is performed 3-4 times, each time for 30-60 minutes, and the filtrates are combined after each extraction.

[0056] Secondly, the present invention also provides a method for preparing a traditional Chinese medicine tea for all seasons, specifically including the following operations:

[0057] Weigh out each ingredient in the above-mentioned spring medicinal tea according to the specified weight proportions, crush and mix them, and place them in a tea bag to obtain the spring medicinal tea.

[0058] Weigh out each ingredient in the above-mentioned summer medicinal tea according to the specified weight proportions, crush and mix them, and place them in a tea bag to obtain the summer medicinal tea.

[0059] Weigh out each ingredient in the above-mentioned autumn medicinal tea according to the specified weight proportions, crush and mix them, and place them in a tea bag to obtain the autumn medicinal tea.

[0060] Weigh out each ingredient in the above-mentioned winter medicinal tea according to the specified weight proportions, crush and mix them, and place them in a tea bag to obtain the winter medicinal tea.

[0061] By combining and packaging the spring herbal tea, summer herbal tea, autumn herbal tea, and winter herbal tea, the four-season Chinese herbal tea is obtained. Detailed Implementation

[0062] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to specific embodiments. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the invention.

[0063] Herbal teas are a common way to utilize Chinese medicinal herbs that are both food and medicine. They improve health by leveraging the effects of compounded herbs. These herbs are still considered traditional Chinese medicine, and their ideal effects only occur when properly combined and suited to the individual's condition. Therefore, rational combination is essential. These herbs are generally mild in nature, and herbal teas, often brewed, contain very few medicinal components, making it difficult to achieve the full medicinal effect they would have as a medicine. Furthermore, the complexity of Chinese medicinal components means that extracts will vary depending on the extraction method, resulting in different and unpredictable effects. Therefore, extraction methods cannot be chosen arbitrarily. Finally, Chinese medicinal herbs often have unpleasant odors and tastes; adding flavoring agents to improve these would contradict the pursuit of health.

[0064] To obtain a traditional Chinese medicine tea that can effectively improve the body's health and has a pleasant aroma, this invention provides a four-season traditional Chinese medicine tea, including spring, summer, autumn, and winter teas. The spring tea comprises the following ingredients in parts by weight: 2-4 parts chrysanthemum, 1-3 parts goji berries, 1-3 parts white peony extract, 1-3 parts golden lotus, and 1-3 parts licorice polysaccharide. The summer tea comprises the following ingredients in parts by weight: 2-4 parts longan pulp, 2-4 parts lily bulb... The autumn herbal tea contains 1-3 parts of Lophatherum gracile extract, 2-4 parts of Poria cocos, and 1-3 parts of licorice polysaccharide. The winter herbal tea contains 4-6 parts of dried ginger, 1-3 parts of Cyperus rotundus extract, 5-7 parts of Polygonatum sibiricum, 4-6 parts of Cornus officinalis, 1-3 parts of ginseng, 9-11 parts of jujube, and 1-3 parts of licorice polysaccharide. The ingredients in this seasonal herbal tea, such as wolfberry and white peony root, are all food-grade medicinal materials and are safe for the body. Based on the characteristics of different seasons and the human body's response to the seasons and the physical conditions that are prone to occur, this invention, through the combination of various ingredients in the above-mentioned medicinal teas, can regulate immunity, improve allergy symptoms, reduce dampness, improve appetite, dry dampness and resolve phlegm, and relieve constipation in all four seasons, thereby improving the user's physical condition and enhancing their quality of life.

[0065] In this embodiment, the preferred proportions of the ingredients in the spring herbal tea are: 2.5-3.5 parts chrysanthemum, 1.5-2.5 parts goji berries, 1.5-2.5 parts white peony extract, 1.5-2.5 parts golden lotus, and 1.5-2.5 parts licorice polysaccharide. A more preferred proportion is: 3 parts chrysanthemum, 2 parts goji berries, 2 parts white peony extract, 2 parts golden lotus, and 2 parts licorice polysaccharide.

[0066] In this embodiment, the preferred proportions of the ingredients in the summer herbal tea are: 2.5-3.5 parts longan pulp, 2.5-3.5 parts lily bulb, 1.5-2.5 parts bamboo leaf extract, 2.5-3.5 parts poria cocos, and 1.5-2.5 parts licorice polysaccharide. A more preferred proportion is: 3 parts longan pulp, 3 parts lily bulb, 2 parts bamboo leaf extract, 3 parts poria cocos, and 2 parts licorice polysaccharide.

[0067] In this embodiment of the application, the preferred proportions of the raw materials in the autumn herbal tea are: 2.5-3.5 parts of Ophiopogon japonicus, 1.5-2.5 parts of Citrus reticulata peel, 2.5-3.5 parts of Sterculia lychnophora, 1.5-2.5 parts of Acanthopanax senticosus extract, and 1.5-2.5 parts of Astragalus membranaceus polysaccharide. A more preferred proportion is: 3 parts of Ophiopogon japonicus, 2 parts of Citrus reticulata peel, 3 parts of Sterculia lychnophora, 2 parts of Acanthopanax senticosus extract, and 2 parts of Astragalus membranaceus polysaccharide.

[0068] In this embodiment, the preferred proportions of the ingredients in the winter herbal tea are: 4.5-5.5 parts dried ginger, 1.5-2.5 parts Cyperus rotundus extract, 5.5-6.5 parts Polygonatum sibiricum, 4.5-5.5 parts Cornus officinalis, 1.5-2.5 parts ginseng, 9.5-10.5 parts jujube, and 1.5-2.5 parts licorice polysaccharide. A more preferred proportion is: 5 parts dried ginger, 2 parts Cyperus rotundus extract, 6 parts Polygonatum sibiricum, 5 parts Cornus officinalis, 2 parts ginseng, 10 parts jujube, and 2 parts licorice polysaccharide.

[0069] To better improve the body's health level and taste, in this embodiment of the application, the preparation method of the white peony extract is as follows: white peony is ultrasonically extracted with ethanol for 0.5 to 1 hour, followed by solid-liquid separation to obtain ethanol-extracted white peony residue. The obtained residue is then refluxed with 0.1 to 0.3 mol / L sodium carbonate aqueous solution for extraction. The obtained extract is then concentrated and dried to obtain the extract.

[0070] The preparation method of Lophatherum gracile leaf extract is as follows: Lophatherum gracile leaves are extracted by reflux with 0.05-0.15 mol / L hydrochloric acid aqueous solution, and the resulting extract is concentrated and dried to obtain the extract.

[0071] The preparation method of Acanthopanax senticosus extract is as follows: Acanthopanax senticosus is extracted by reflux with 0.05-0.15 mol / L hydrochloric acid aqueous solution, the resulting extract is concentrated and dried to obtain Acanthopanax senticosus aqueous extract and Acanthopanax senticosus residue; Acanthopanax senticosus residue is extracted by reflux with 75%-85% v / v ethanol, the resulting extract is concentrated and dried to obtain Acanthopanax senticosus ethanol extract; the Acanthopanax senticosus aqueous extract and Acanthopanax senticosus ethanol extract are mixed to obtain the final product.

[0072] The preparation method of Astragalus polysaccharide is as follows: Astragalus is extracted by reflux with water to obtain reflux extract, which is concentrated and ethanol is added to the concentration of ethanol to 55% to 65% v / v. After standing and precipitation, the supernatant is separated, and the obtained supernatant is concentrated and dried to obtain the polysaccharide.

[0073] The preparation method of Cyperus rotundus extract is as follows: Cyperus rotundus is extracted with 85% to 100% v / v ethanol by ultrasonic extraction, and the resulting extract is concentrated and dried to obtain the extract.

[0074] The preparation method of licorice polysaccharide is as follows: licorice is extracted with water by ultrasonic extraction to obtain ultrasonic extract and licorice residue; the obtained licorice residue is then extracted with water by reflux to obtain reflux extract; the ultrasonic extract and reflux extract are combined, concentrated, and ethanol is added to an ethanol concentration of 75% to 85% v / v. After standing and precipitation, the supernatant is separated, and the obtained supernatant is concentrated and dried to obtain the licorice polysaccharide.

[0075] This invention combines the effects of improving the body's health with the aroma and flavor of medicinal tea, and provides a method for preparing the aforementioned medicinal tea, specifically including the following steps:

[0076] Weigh out the ingredients for the spring, summer, autumn, and winter medicinal teas according to the specified weight proportions, crush and mix them, and place them in tea bags to obtain the seasonal medicinal teas. Combine and package the seasonal medicinal teas to obtain the four-season Chinese medicinal tea.

[0077] The embodiments of the present invention will be further described below with reference to several examples, and some comparative experiments and animal experiments are listed to demonstrate the effectiveness of the present invention in many aspects.

[0078] The chrysanthemum, wolfberry, white peony root, licorice root, longan pulp, lily bulb, light bamboo leaf, poria cocos, ophiopogon japonicus, tangerine peel, sterculia lychnophora, eleutherococcus senticosus, dried ginger, cyperus rotundus, polygonatum sibiricum, cornus officinalis, ginseng, and jujube in the following examples all meet the quality standards of the Chinese Pharmacopoeia (2020 edition), and the golden lotus flower meets the Hubei Provincial Standards for the Quality of Traditional Chinese Medicine (2018 edition).

[0079] Examples 1-5

[0080] Embodiments 1-5 of this invention provide a traditional Chinese medicine tea for all four seasons. The raw materials for each embodiment are shown in Table 1:

[0081] Table 1. Raw material ratios for Examples 1-5

[0082]

[0083]

[0084] The preparation methods of the white peony extract, light bamboo leaf extract, Acanthopanax senticosus extract, astragalus polysaccharide, cyperus extract, and licorice polysaccharide in Examples 1-5 above are as follows:

[0085] White peony extract: White peony is ultrasonically extracted with 5 times the amount of ethanol for 0.5-1h, followed by solid-liquid separation to obtain ethanol-extracted white peony residue. The residue is then refluxed with 5 times the amount of 0.1-0.3mol / L sodium carbonate aqueous solution for 2-3 times, each time for 30-45min. The resulting extract is then concentrated and dried to obtain the final product.

[0086] Lophatherum gracile leaf extract: Lophatherum gracile leaves were refluxed with 4 times the amount of 0.05-0.15 mol / L hydrochloric acid aqueous solution 2-3 times, each time for 30-45 min. The resulting extract was concentrated and dried to obtain the extract.

[0087] Acanthopanax senticosus extract: Acanthopanax senticosus is refluxed with 4 times the volume of 0.05-0.15 mol / L hydrochloric acid aqueous solution for 2-3 times, 30-45 min each time. The resulting extract is concentrated and dried to obtain the aqueous extract of Acanthopanax senticosus and the residue of Acanthopanax senticosus. The residue of Acanthopanax senticosus is refluxed with 4 times the volume of 75%-85% v / v ethanol for 2-3 times, 30-45 min each time. The resulting extract is concentrated and dried to obtain the ethanolic extract of Acanthopanax senticosus. The aqueous extract and the ethanolic extract of Acanthopanax senticosus are mixed to obtain the final product.

[0088] Astragalus polysaccharide: Extract astragalus by reflux with 10 times the amount of water, 3 to 5 times, 60 to 90 minutes each time. Concentrate the obtained extract, add ethanol to the concentration of 55% to 65% v / v, let stand (24 h) to precipitate, separate the supernatant, concentrate and dry the obtained supernatant to obtain the polysaccharide.

[0089] Cyperus rotundus extract: Cyperus rotundus is extracted with 6 times the amount of 85% to 100% v / v ethanol at 600 to 800 W power and ultrasonically extracted at 40 to 60 °C for 2 to 3 times, each time for 60 to 90 min. The resulting extract is concentrated and dried to obtain the extract.

[0090] Licorice extract: Licorice was ultrasonically extracted with 12 times its volume of water at 600-800W and 50-60℃ for 3-4 times, 60-90 min each time. After each extraction, the extract was filtered and the filtrates were combined to obtain ultrasonic extract and licorice residue. The licorice residue was then refluxed with 12 times its volume of water for 3-4 times, 30-60 min each time to obtain reflux extract. The ultrasonic extract and reflux extract were combined, concentrated, and ethanol was added to a concentration of 75%-85% v / v. After standing (24 h) for precipitation, the supernatant was separated. The supernatant was concentrated and dried to obtain the final extract.

[0091] The specific parameters are shown in Table 2:

[0092] Table 2 shows the specific parameters for preparing each extract or polysaccharide in Examples 1-5.

[0093]

[0094]

[0095] The preparation methods for Examples 1 to 5 above are as follows:

[0096] Weigh out the ingredients for the above-mentioned spring, summer, autumn, and winter medicinal teas according to the specified weight proportions, crush and mix them, place them in tea bags, and you will get the medicinal teas for each season. After combining and packaging, you will get the Chinese medicinal tea for all four seasons.

[0097] Comparative Example 1

[0098] This comparative example provides a spring herbal tea: based on Example 1, the golden lotus in the spring herbal tea is replaced with honeysuckle, and the other ingredients and preparation method are the same as in Example 1.

[0099] Comparative Example 2

[0100] This comparative example provides a spring herbal tea: Based on Example 1, the preparation method of the white peony extract in the spring herbal tea is changed to: white peony is extracted twice by reflux with 0.2 mol / L sodium carbonate aqueous solution, the first time for 45 min and the second time for 30 min. The resulting extract is concentrated and dried to obtain the tea.

[0101] The other components and preparation methods are the same as in Example 1.

[0102] Comparative Example 3

[0103] This comparative example provides a summer herbal tea: based on Example 1, the Poria cocos in the summer herbal tea is replaced with Coix seed, and the other ingredients and preparation method are the same as in Example 1.

[0104] Comparative Example 4

[0105] This comparative example provides a summer herbal tea: Based on Example 1, the preparation method of the bamboo leaf extract in the summer herbal tea is changed to: bamboo leaves are extracted twice by reflux with aqueous solution, the first time for 45 minutes and the second time for 30 minutes, and the resulting extract is concentrated and dried to obtain the tea.

[0106] The other components and preparation methods are the same as in Example 1.

[0107] Comparative Example 5

[0108] This comparative example provides an autumn herbal tea: based on Example 1, the malva nut in the autumn herbal tea is replaced with raw licorice, and the other ingredients and preparation method are the same as in Example 1.

[0109] Comparative Example 6

[0110] This comparative example provides an autumn herbal tea: Based on Example 1, the preparation method of Acanthopanax senticosus extract in the autumn herbal tea is changed to: Acanthopanax senticosus residue is extracted by reflux with 4 times the amount of 75% to 85% v / v ethanol, and the resulting extract is concentrated and dried to obtain the final product.

[0111] The other components and preparation methods are the same as in Example 1.

[0112] Comparative Example 7

[0113] This comparative example provides a winter herbal tea: based on Example 1, the Cyperus rotundus extract in the winter herbal tea is replaced with Curcuma longa extract, the extraction method is the same, and other components and preparation methods are the same as in Example 1.

[0114] Comparative Example 8

[0115] This comparative example provides a winter medicinal tea: based on Example 1, the preparation method of Cyperus rotundus extract in the winter medicinal tea is changed to: extracting Cyperus rotundus with water using ultrasound, concentrating and drying the resulting extract to obtain the tea.

[0116] Example 1

[0117] This effect study examines the effects of spring herbal teas from various embodiments and comparative examples on immunity.

[0118] 1. Materials and Methods

[0119] 1.1 Materials

[0120] Take 10g each of the spring herbal tea from Example 1 and the spring herbal teas from Comparative Examples 1 and 2, soak them in 100mL of distilled water at 80-90℃ for 30min, filter, and use the filtrate as the test solution for the dosage group in Example 1 and the test solutions for Comparative Examples 1 and 2 (concentration based on raw material, 0.1g / ml). Take another 5g of the spring herbal tea from Example 1, soak it in 100mL of distilled water at 80-90℃ for 30min, filter, and use the filtrate as the test solution for the low-dose group in Example 1 (concentration based on raw material, 0.05g / ml). Take another 15g of the spring herbal tea from Example 1, soak it in 100mL of distilled water at 80-90℃ for 30min, filter, and use the filtrate as the test solution for the high-dose group in Example 1 (concentration based on raw material, 0.15g / ml).

[0121] The experimental animals were healthy male Kunming mice, weighing 18–22 g. They were acclimatized for one week under environmental conditions of 20–24 °C and 50–65% humidity before subsequent experiments were conducted under the same conditions.

[0122] 1.2 Methods

[0123] Mice were randomly divided into four groups: the low-dose group (Example 1), the medium-dose group (Example 1), the high-dose group (Example 1), Comparative Example 1, Comparative Example 2, the model group, and the blank control group, with 10 mice in each group. Except for the blank group, all mice were intraperitoneally injected with cyclophosphamide solution (10 mg / kg / day) once daily for three consecutive days. Drug administration was then initiated, with intraperitoneal injections of cyclophosphamide solution (10 mg / kg / day) every 7 days during the drug administration period. The blank group and the model group were administered distilled water by gavage. The low-dose group, the medium-dose group, the high-dose group, Comparative Example 1, and Comparative Example 2 were administered the corresponding test solutions by gavage. The gavage volume for each group was 10 ml / kg, administered once daily for 30 consecutive days. After 30 days of continuous gavage, various indicators were tested according to the relevant provisions of the "Detection Method for Enhanced Immune Function" in the "Technical Specifications for Inspection and Evaluation of Health Foods (2003 Edition)". During the experiment, all mice were given standard mouse feed and distilled water, with free access to food.

[0124] 2. Results

[0125] SPSS 18.0 was used to perform analysis of variance on the experimental data. The results are expressed as mean ± standard deviation (X±s). The t-test was used, and P<0.05 was considered statistically significant.

[0126] 2.1 Immune organ / body weight ratio

[0127] After the experiment, the mice were weighed and euthanized. The thymus and spleen were weighed, and the organ / body weight ratio was calculated. The results are shown in Table 3.

[0128] Table 3. Immune Organ / Body Weight Ratio

[0129] Group n Spleen / body weight (%) Thymus / body weight (%) Blank group 10 <![CDATA[0.432±0.052 b ]]> <![CDATA[0.356±0.029 b ]]> Model group 10 <![CDATA[0.337±0.039 a ]]> <![CDATA[0.216±0.043 a ]]> Example 1 Low-dose group 10 <![CDATA[0.487±0.041 ab ]]> <![CDATA[0.402±0.051 ab ]]> In Example 1, the dosage group 10 <![CDATA[0.509±0.029 ab ]]> <![CDATA[0.417±0.034 ab ]]> Example 1 High-dose group 10 <![CDATA[0.518±0.037 ab ]]> <![CDATA[0.428±0.037 ab ]]> Comparative Example 1 10 <![CDATA[0.472±0.042 abc ]]> <![CDATA[0.404±0.045 abc ]]> Comparative Example 2 10 <![CDATA[0.483±0.057 abc ]]> <![CDATA[0.401±0.036 abc ]]>

[0130] Note: a indicates comparison with the blank group, P<0.05; b indicates comparison with the model group, P<0.05; c indicates comparison with the dosage group in Example 1, P<0.05.

[0131] 2.2 Delayed-type hypersensitivity (DTH)

[0132] Five days before the end of the experiment, the skin of the mouse abdomen was shaved in an area of ​​about 3×3cm. 50μl of DNFB solution was applied evenly to sensitize the mouse. Five days later, 10μl of DNFB solution was applied evenly to both sides of the mouse's right ear. The mouse was sacrificed by cervical spine 24 hours later. The left and right auricles were cut off, and 2mm diameter ear pieces were taken and weighed. The results are shown in Table 4.

[0133] Table 4. DTH Experimental Results

[0134]

[0135]

[0136] Note: a indicates comparison with the blank group, P<0.05; b indicates comparison with the model group, P<0.05; c indicates comparison with the dosage group in Example 1, P<0.05.

[0137] 2.3 Splenic lymphocyte transformation

[0138] Spleens were collected from euthanized mice and a cell suspension was prepared, with the cell concentration adjusted to approximately 3 × 10⁻⁶. 6 Lymphocytes were counted at 1 / ml using the MTT assay. The difference in optical density between the wells with and without ConA was used to assess lymphocyte proliferation. The results are shown in Table 5.

[0139] Table 5 Cell transformation and proliferation capacity

[0140]

[0141] Note: a indicates comparison with the blank group, P<0.05; b indicates comparison with the model group, P<0.05; c indicates comparison with the dosage group in Example 1, P<0.05.

[0142] 2.4 NK cell activity

[0143] Take mouse spleen cell suspension and adjust the cell concentration to 2×10⁻⁶. 7 YAC-1 cells were selected at a concentration of 4 × 10⁶ cells / ml as effector cells; well-grown YAC-1 cells were collected and the cell concentration was adjusted to 4 × 10⁶ cells / ml. 5 NK cell activity was measured by using cells per ml as target cells and performing the LDH assay.

[0144] NK cell activity (%) = (OD 反应孔 -OD 自然释放孔 ) / (OD 最大释放孔 -OA 自然释放孔 ()×100%. The results are shown in Table 6.

[0145] Table 6 NK cell activity

[0146] Group n NK cell activity (%) Blank group 10 <![CDATA[32.32±0.018 b ]]> Model group 10 <![CDATA[26.26±0.023 a <!-- 11 -->]]> Example 1 Low-dose group 10 <![CDATA[37.10±0.032 ab ]]> In Example 1, the dosage group 10 <![CDATA[45.26±0.050 ab ]]> Example 1 High-dose group 10 <![CDATA[49.57±0.049 ab ]]> Comparative Example 1 10 <![CDATA[36.72±0.026 abc ]]> Comparative Example 2 10 <![CDATA[36.14±0.019 abc ]]>

[0147] Note: a indicates comparison with the blank group, P<0.05; b indicates comparison with the model group, P<0.05; c indicates comparison with the dosage group in Example 1, P<0.05.

[0148] As can be seen from the above results, the spring herbal tea obtained in Example 1 of the present invention can effectively enhance the body's immunity, and this effect is better than that of Comparative Example 1 and Comparative Example 2.

[0149] Take 10g of each of the spring herbal teas from Examples 2-5 and soak them in 100mL of distilled water at 80-90℃ for 30min. Filter the solution and use the filtrate as the test solution for each example (concentration based on raw material, 0.1g / ml). Detect the effect of the spring herbal teas from Examples 2-5 on immunity according to the above method. The results are shown in Tables 7-10.

[0150] Table 7. Immune organ / body weight ratio in mice of Examples 2-5

[0151] Group n Spleen / body weight (%) Thymus / body weight (%) Blank group 10 <![CDATA[0.419±0.047 b ]]> <![CDATA[0.352±0.033 b ]]> Model group 10 <![CDATA[0.329±0.039 a ]]> <![CDATA[0.219±0.038 a ]]> Example 2 group 10 <![CDATA[0.495±0.023 ab ]]> <![CDATA[0.405±0.043 ab ]]> Example 3 Group 10 <![CDATA[0.501±0.031 ab ]]> <![CDATA[0.403±0.031 ab ]]> Example 4 group 10 <![CDATA[0.487±0.026 ab ]]> <![CDATA[0.412±0.035 ab ]]> Example 5 group 10 <![CDATA[0.488±0.019 ab ]]> <![CDATA[0.407±0.029 ab ]]>

[0152] Note: a indicates comparison with the blank group, P<0.05; b indicates comparison with the model group, P<0.05.

[0153] Table 8 Results of DTH experiments in mice in Examples 2-5

[0154] Group n Weight difference between left and right earpieces (mg) Blank group 10 <![CDATA[7.05±2.25 b ]]> Model group 10 <![CDATA[16.07±3.26 a ]]> Example 2 group 10 <![CDATA[9.78±2.01 ab ]]> Example 3 Group 10 <![CDATA[9.79±2.52 ab ]]> Example 4 group 10 <![CDATA[9.72±2.37 ab ]]> Example 5 group 10 <![CDATA[9.75±3.01 ab ]]>

[0155] Note: a indicates comparison with the blank group, P<0.05; b indicates comparison with the model group, P<0.05.

[0156] Table 9. Cell transformation and proliferation capacity of mouse groups 2-5 in Examples 2-5

[0157]

[0158] Note: a indicates comparison with the blank group, P<0.05; b indicates comparison with the model group, P<0.05.

[0159] Table 10. NK cell activity in mice from Examples 2-5

[0160] Group n NK cell activity (%) Blank group 10 <![CDATA[31.97±0.024 b ]]> Model group 10 <![CDATA[25.77±0.032 a ]]> Example 2 group 10 <![CDATA[44.62±0.037 ab ]]> Example 3 Group 10 <![CDATA[43.72±0.047 ab ]]> Example 4 group 10 <![CDATA[44.79±0.042 ab ]]> Example 5 group 10 <![CDATA[44.35±0.039 ab ]]>

[0161] Note: a indicates comparison with the blank group, P<0.05; b indicates comparison with the model group, P<0.05.

[0162] As can be seen from the above results, the spring herbal teas obtained in Examples 2 to 5 of the present invention can also effectively enhance the body's immunity.

[0163] Example 2

[0164] This effect study examines the anti-allergic effects of the spring herbal teas in the various embodiments and comparative examples.

[0165] 1. Materials and Methods

[0166] 1.1 Materials

[0167] Take 10g each of the spring herbal tea from Example 1 and the spring herbal teas from Comparative Examples 1 and 2, soak them in 100mL of distilled water at 80-90℃ for 30min, filter, and use the filtrate as the test solution for Example 1 and the test solutions for Comparative Examples 1 and 2 (concentration based on raw materials, 0.1g / ml).

[0168] The experimental animals were healthy male Kunming mice, weighing 18–22 g. They were acclimatized for one week under environmental conditions of 20–24 °C and 50–65% humidity before subsequent experiments were conducted under the same conditions.

[0169] 1.2 Methods

[0170] Experimental mice were randomly divided into four groups: Example 1, Comparative Example 1, Comparative Example 2, Model Group, and Blank Control Group, with 10 mice in each group. After weighing and blood collection from the tail artery, the blank control group and model group were administered distilled water by gavage. Mice in Example 1, Comparative Example 1, and Comparative Example 2 groups were administered the corresponding test solutions by gavage. The gavage volume for each group was 10 ml / kg, administered once daily. On days 8, 22, 29, and 36 after the start of gavage, mice in Example 1, Comparative Example 1, Comparative Example 2, and Model Groups were intraperitoneally injected with a PBS solution containing ovalbumin (400 μg / ml ovalbumin and 40 mg / ml aluminum hydroxide), with each mouse receiving 0.1 ml per injection. Throughout the experiment, all mice were fed standard mouse feed and distilled water, with free access to food and water.

[0171] Seven days after the last intraperitoneal injection, the patient was weighed, and blood was drawn from behind the eyeball to measure the serum IgE concentration.

[0172] 2. Results

[0173] SPSS 18.0 was used to perform analysis of variance on the experimental data. The results are expressed as mean ± standard deviation (X±s). The t-test was used, and P<0.05 was considered statistically significant.

[0174] The results are shown in Table 11.

[0175] Table 11. Mouse serum IgE (ng / ml)

[0176]

[0177]

[0178] Note: a indicates comparison with the blank group, P<0.05; b indicates comparison with the model group, P<0.05; c indicates comparison with Example 1 group, P<0.05.

[0179] As can be seen from the above results, the spring herbal tea obtained in Example 1 of the present invention can reduce IgE levels, thereby reducing allergic reactions in the body, and this effect is better than that of Comparative Example 1 and Comparative Example 2.

[0180] Take 10g of each of the spring herbal teas from Examples 2-5 and soak them in 100mL of distilled water at 80-90℃ for 30min. Filter the solution and use the filtrate as the test solution for each example (concentration based on raw material, 0.1g / ml). Test the anti-allergic effects of the spring herbal teas from Examples 2-5 according to the above method. The results are shown in Table 12.

[0181] Table 12 Serum IgE (ng / ml) in mice from Examples 2-5

[0182] Group n Before administration After administration Blank group 10 3.22±1.29 <![CDATA[12.08±7.32 b ]]> Model group 10 3.45±1.03 <![CDATA[53.64±4.92 a ]]> Example 2 group 10 3.23±0.82 <![CDATA[24.89±8.23 ab ]]> Example 3 Group 10 3.41±0.67 <![CDATA[25.32±5.32 ab ]]> Example 4 group 10 3.35±1.56 <![CDATA[25.77±6.53 ab ]]> Example 5 group 10 3.36±0.71 <![CDATA[26.43±7.32 ab ]]>

[0183] Note: a indicates comparison with the blank group, P<0.05; b indicates comparison with the model group, P<0.05.

[0184] As can be seen from the above results, the spring herbal teas obtained in Examples 2 to 5 of the present invention also have anti-allergic effects.

[0185] Example 3

[0186] This effect study examines the effects of the summer herbal teas in the various embodiments and comparative examples on improving humidity levels.

[0187] 1. Materials and Methods

[0188] 1.1 Materials

[0189] Take 10g each of the summer herbal tea from Example 1 and the summer herbal teas from Comparative Examples 3 and 4, and soak them in 100mL of distilled water at 80-90℃ for 30min. Filter the solution and use the filtrate as the test solution for the dosage group in Example 1 and the test solutions for Comparative Examples 3 and 4 (concentration based on raw material, 0.1g / ml). Separately, take 5g of the summer herbal tea from Example 1, soak it in 100mL of distilled water at 80-90℃ for 30min, and filter the solution. Use the filtrate as the test solution for the low-dose group in Example 1 (concentration based on raw material, 0.05g / ml). Separately, take 15g of the summer herbal tea from Example 1, soak it in 100mL of distilled water at 80-90℃ for 30min, and filter the solution. Use the filtrate as the test solution for the high-dose group in Example 1 (concentration based on raw material, 0.15g / ml).

[0190] The experimental animals were healthy male Kunming mice, weighing 18–22g. They were acclimatized for one week under environmental conditions of 20–24℃ and 50–65% humidity before the subsequent experiments were completed.

[0191] 1.2 Methods

[0192] Experimental mice were randomly divided into four groups: the low-dose group (Example 1), the medium-dose group (Example 1), the high-dose group (Example 1), Comparative Example 3, Comparative Example 4, the model group, and the blank control group, with 10 mice in each group. After weighing, the temperature of the enclosures for the mice in the low-dose group (Example 1), the medium-dose group (Example 1), the high-dose group (Example 1), Comparative Example 3, Comparative Example 4, and the model group was increased to 35±1℃, and the humidity was increased to 80-90%. The drinking water was changed to 20% sugar water, and the feed was changed to high-protein feed. The mice in the blank control group continued to receive normal mouse feed and distilled water. All mice had free access to food. After 6 weeks, the blank control group and the model group were administered distilled water by gavage, while the mice in the low-dose group (Example 1), the medium-dose group (Example 1), the high-dose group (Example 1), Comparative Example 3, and Comparative Example 4 were administered the corresponding test solutions by gavage. The gavage volume for each group was 10 ml / kg, administered once daily for 30 consecutive days. During the drug administration period, all mice continued to receive their respective diets and had free access to food.

[0193] 2. Results

[0194] SPSS 18.0 was used to perform analysis of variance on the experimental data. The results are expressed as mean ± standard deviation (X±s). The t-test was used, and P<0.05 was considered statistically significant.

[0195] Before administration by gavage, except for the control group, the mice in the other groups showed lethargy, fatigue, dull fur, dark yellow fur around the tail and anus, and tail dragging during defecation. Their food and water intake were also significantly reduced compared to the control group. The body weight and average food intake of the mice in each group on the day before administration and on the day after administration (30 days) are shown in Table 13.

[0196] Table 13 Body weight and average food intake

[0197]

[0198]

[0199] Note: a indicates comparison with the blank group, P<0.05; b indicates comparison with the model group, P<0.05; c indicates comparison with the dosage group in Example 1, P<0.05.

[0200] In Example 1, the mental state and coat condition of mice in all groups and in Comparative Examples 3 and 4 were significantly restored. In Example 1, the mice in the medium-dose group and the high-dose group were basically restored to the same level as the blank group.

[0201] The results above show that the food intake of the model group mice was significantly lower than that of the control group, and their weight still decreased even when their diet contained sugars that easily lead to weight gain. The decrease in food intake and weight of the model group mice may also be related to their depressed mood and decreased spleen and stomach function caused by damp heat. The summer herbal tea obtained in Example 1 of this invention can promote appetite in mice, improve various symptoms caused by damp heat, and gradually restore their weight to normal, and this effect is significantly better than that of Comparative Examples 3 and 4.

[0202] Take 10g of each of the summer herbal teas from Examples 2-5 and soak them in 100mL of distilled water at 80-90℃ for 30min. Filter the solution and use the filtrate as the test solution for each example (concentration based on raw material, 0.1g / ml). Test the effect of the summer herbal teas from Examples 2-5 on improving humidity using the above method. The results are shown in Table 14.

[0203] Table 14. Body weight and average food intake of mice in groups 2-5 of Examples 14

[0204]

[0205] Note: a indicates comparison with the blank group, P<0.05; b indicates comparison with the model group, P<0.05.

[0206] As can be seen from the above results, the summer medicinal teas obtained in Examples 2 to 5 of the present invention also have the effect of improving humidity.

[0207] Example of effect 4

[0208] This effect study examines the expectorant effects of the autumn herbal teas in the various embodiments and comparative examples.

[0209] 1. Materials and Methods

[0210] 1.1 Materials

[0211] Take 10g each of the autumn herbal tea from Example 1 and the autumn herbal teas from Comparative Examples 5 and 6, and soak them in 100mL of distilled water at 80-90℃ for 30min. Filter the solution and use the filtrate as the test solution for the dosage group in Example 1 and the test solutions for Comparative Examples 5 and 6 (concentration based on raw material, 0.1g / ml). Separately, take 5g of the autumn herbal tea from Example 1, soak it in 100mL of distilled water at 80-90℃ for 30min, and filter the solution. Use the filtrate as the test solution for the low-dose group in Example 1 (concentration based on raw material, 0.05g / ml). Separately, take 15g of the autumn herbal tea from Example 1, soak it in 100mL of distilled water at 80-90℃ for 30min, and filter the solution. Use the filtrate as the test solution for the high-dose group in Example 1 (concentration based on raw material, 0.15g / ml).

[0212] The experimental animals were healthy male Kunming mice, weighing 18–22g. They were acclimatized for one week under environmental conditions of 20–24℃ and 20–30% humidity before subsequent experiments were conducted under the same conditions.

[0213] 1.2 Methods

[0214] Experimental mice were randomly divided into four groups: the low-dose group (Example 1), the medium-dose group (Example 1), the high-dose group (Example 1), comparative group 5, comparative group 6, and a blank control group, with 10 mice in each group. The blank control group was administered distilled water by gavage. Mice in the low-dose group (Example 1), the medium-dose group (Example 1), the high-dose group (Example 1), comparative group 5, and comparative group 6 were administered the corresponding test solutions by gavage. The gavage volume for each group was 10 ml / kg. Gavage was performed once a day for 30 consecutive days. Four hours after the last gavage, 5% phenol red solution (0.1 ml / 10 g body weight) was injected intraperitoneally. Thirty minutes later, the mice were euthanized by cervical dislocation. The trachea was separated from the neck and repeatedly lavaged three times with 5% NaHCO3 solution. The lavage fluids were combined, centrifuged, and the OD value of the supernatant at 546 nm was measured. The amount of phenol red in the supernatant was calculated using the standard curve method.

[0215] 2. Results

[0216] SPSS 18.0 was used to perform analysis of variance on the experimental data. The results are expressed as mean ± standard deviation (X±s). The t-test was used, and P<0.05 was considered statistically significant.

[0217] The results are shown in Table 15.

[0218] Table 15 OD values ​​and phenol red excretion

[0219] Group n OD value Phenol red excretion (μg / ml) Blank group 10 0.129±0.0181 0.072±0.0031 Example 1 Low-dose group 10 <![CDATA[0.156±0.0243 a ]]> <![CDATA[0.075±0.0049 a ]]> In Example 1, the dosage group 10 <![CDATA[0.162±0.0352 a ]]> <![CDATA[0.078±0.0059 a ]]> Example 1 High-dose group 10 <![CDATA[0.168±0.0213 a ]]> <![CDATA[0.081±0.0062 a ]]> Comparative Example 1 10 <![CDATA[0.139±0.0521 a ]]> <![CDATA[0.074±0.0051 ab ]]> Comparative Example 2 10 <![CDATA[0.142±0.0193 a ]]> <![CDATA[0.074±0.0069 ab ]]>

[0220] Note: a indicates comparison with the blank group, P<0.05; b indicates comparison with the dosage group in Example 1, P<0.05.

[0221] As can be seen from the above results, the autumn herbal tea obtained in Example 1 of the present invention can promote the excretion of phenol red from the bronchi, indicating that the autumn herbal tea has an expectorant effect, and this effect is better than that of comparative examples 5 and 6.

[0222] Take 10g of each of the autumn herbal teas from Examples 2-5 and soak them in 100mL of distilled water at 80-90℃ for 30min. Filter the solution and use the filtrate as the test solution for each example (concentration based on raw material, 0.1g / ml). Test the expectorant effect of the autumn herbal teas from Examples 2-5 according to the above method. The results are shown in Table 16.

[0223] Table 16 OD values ​​and phenol red excretion of mice in groups 2-5 of Examples

[0224] Group n OD value Phenol red excretion (μg / ml) Blank group 10 0.127±0.0162 0.072±0.0048 Example 2 group 10 <![CDATA[0.160±0.0191 a ]]> <![CDATA[0.077±0.0064 a ]]> Example 3 Group 10 <![CDATA[0.159±0.0216 a ]]> <![CDATA[0.077±0.0032 a ]]> Example 4 group 10 <![CDATA[0.155±0.03943 a ]]> <![CDATA[0.076±0.0063 a ]]> Example 5 group 10 <![CDATA[0.157±0.0376 a ]]> <![CDATA[0.077±0.0071 a ]]>

[0225] Note: 'a' indicates comparison with the blank group, P<0.05.

[0226] Example 5

[0227] This effect study examines the effects of the winter herbal teas in the various embodiments and comparative examples on improving constipation.

[0228] 1. Materials and Methods

[0229] 1.1 Materials

[0230] Take 10g each of the winter herbal tea from Example 1 and the winter herbal teas from Comparative Examples 7 and 8, and soak them in 100mL of distilled water at 80-90℃ for 30min. Filter the solution and use the filtrate as the test solution for the dosage group in Example 1 and the test solutions for Comparative Examples 7 and 8 (concentration based on raw material, 0.1g / ml). Separately, take 5g of the winter herbal tea from Example 1, soak it in 100mL of distilled water at 80-90℃ for 30min, and filter the solution. Use the filtrate as the test solution for the low-dose group in Example 1 (concentration based on raw material, 0.05g / ml). Separately, take 15g of the winter herbal tea from Example 1, soak it in 100mL of distilled water at 80-90℃ for 30min, and filter the solution. Use the filtrate as the test solution for the high-dose group in Example 1 (concentration based on raw material, 0.15g / ml).

[0231] The experimental animals were healthy male Kunming mice, weighing 18–22g. They were acclimatized for one week under environmental conditions of 20–24℃ and 20–30% humidity before subsequent experiments were conducted under the same conditions.

[0232] 1.2 Methods

[0233] Experimental mice were randomly divided into four groups: the low-dose group (Example 1), the medium-dose group (Example 1), the high-dose group (Example 1), comparative group 7, comparative group 8, the model group, and the blank control group, with 10 mice in each group. Except for the blank group, all mice were administered loperamide hydrochloride solution (10 mg / kg) by gavage for 16 consecutive days, once a day, at a dose of 0.1 ml / 10 g body weight. The blank group was administered distilled water by gavage. Mice in the low-dose group (Example 1), the medium-dose group (Example 1), the high-dose group (Example 1), comparative group 7, comparative group 8, and the model group were administered the corresponding test solutions by gavage, with a gavage volume of 10 ml / kg for each group. After 30 minutes, each mouse was administered 0.2 ml of ink by gavage. The time of excretion of the first black feces after ink administration was recorded. Feces excreted by each mouse within 8 hours were collected, and the number of feces, wet weight, and dry weight (dried at 60°C) were recorded. The fecal moisture content was calculated. During the experiment, all mice were given standard mouse feed and distilled water, with free access to food.

[0234] 2. Results

[0235] SPSS 18.0 was used to perform analysis of variance on the experimental data. The results are expressed as mean ± standard deviation (X ± s). The t-test was used, and P < 0.05 was considered statistically significant. The results are shown in Table 17.

[0236] Table 17. Defecation and fecal condition of mice

[0237]

[0238]

[0239] Note: a indicates comparison with the blank group, P<0.05; b indicates comparison with the model group, P<0.05; c indicates comparison with the dosage group in Example 1, P<0.05.

[0240] As can be seen from the above results, the winter herbal tea obtained in Example 1 of the present invention can effectively promote defecation in mice, shorten defecation time, and increase fecal water content, and the above effects are better than those of Comparative Example 7 and Comparative Example 8.

[0241] Take 10g of each of the winter herbal teas from Examples 2-5 and soak them in 100mL of distilled water at 80-90℃ for 30min. Filter the solution and use the filtrate as the test solution for each example (concentration based on raw material, 0.1g / ml). Test the effect of the winter herbal teas from Examples 2-5 on improving constipation according to the above method. The results are shown in Table 18.

[0242] Table 18. Defecation and fecal condition of mice

[0243]

[0244] Note: a indicates comparison with the blank group, P<0.05; b indicates comparison with the model group, P<0.05.

[0245] As can be seen from the above results, the winter medicinal teas obtained in Examples 2 to 5 of the present invention also have the effect of improving constipation.

[0246] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions or improvements made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A type of herbal tea suitable for all four seasons, characterized in that, It consists of spring medicinal tea, summer medicinal tea, autumn medicinal tea, and winter medicinal tea, among which: The spring herbal tea is composed of the following ingredients in the indicated weight proportions: 2-4 parts chrysanthemum, 1-3 parts wolfberry, 1-3 parts white peony extract, 1-3 parts golden lotus, and 1-3 parts licorice polysaccharide; The summer herbal tea is composed of the following ingredients in the indicated weight proportions: 2-4 parts longan pulp, 2-4 parts lily bulb, 1-3 parts light bamboo leaf extract, 2-4 parts poria cocos, and 1-3 parts licorice polysaccharide. The autumn herbal tea is composed of the following ingredients in the indicated weight proportions: 2-4 parts Ophiopogon japonicus, 1-3 parts Citrus reticulata peel, 2-4 parts Sterculia lychnophora, 1-3 parts Acanthopanax senticosus extract, and 1-3 parts Astragalus polysaccharide. The winter herbal tea is composed of the following ingredients in the indicated weight proportions: 4-6 parts dried ginger, 1-3 parts Cyperus rotundus extract, 5-7 parts Polygonatum sibiricum, 4-6 parts Cornus officinalis, 1-3 parts ginseng, 9-11 parts jujube, and 1-3 parts licorice polysaccharide. The preparation method of the white peony extract is as follows: white peony is ultrasonically extracted with ethanol for 0.5-1h, followed by solid-liquid separation to obtain ethanol-extracted white peony residue. The obtained residue is refluxed with 0.1-0.3mol / L sodium carbonate aqueous solution for extraction. The obtained extract is concentrated and dried to obtain the white peony extract. The preparation method of the licorice polysaccharide is as follows: licorice is extracted with water by ultrasonic extraction to obtain ultrasonic extract and licorice residue; the obtained licorice residue is then extracted with water by reflux to obtain reflux extract; the ultrasonic extract and reflux extract are combined, concentrated, and ethanol is added to an ethanol concentration of 75%~85% v / v. After standing and precipitation, the supernatant is separated, and the obtained supernatant is concentrated and dried to obtain the licorice polysaccharide. The preparation method of the Lophatherum gracile extract is as follows: Lophatherum gracile is extracted by reflux with 0.05~0.15mol / L hydrochloric acid aqueous solution, and the resulting extract is concentrated and dried to obtain the Lophatherum gracile extract. The preparation method of the Acanthopanax senticosus extract is as follows: Acanthopanax senticosus is extracted by reflux with 0.05~0.15mol / L hydrochloric acid aqueous solution, the resulting extract is concentrated and dried to obtain Acanthopanax senticosus aqueous extract and Acanthopanax senticosus residue; the Acanthopanax senticosus residue is extracted by reflux with 75%~85%v / v ethanol, the resulting extract is concentrated and dried to obtain Acanthopanax senticosus ethanol extract; the Acanthopanax senticosus aqueous extract and Acanthopanax senticosus ethanol extract are mixed to obtain the Acanthopanax senticosus extract. The preparation method of the Astragalus polysaccharide is as follows: Astragalus is extracted by reflux with water, the resulting extract is concentrated, ethanol is added to the concentration of ethanol to 55%~65% v / v, the supernatant is separated after standing and precipitation, the supernatant is concentrated and dried to obtain the Astragalus polysaccharide. The preparation method of the Cyperus rotundus extract is as follows: Cyperus rotundus is extracted with 85%~100% v / v ethanol by ultrasonic extraction, and the resulting extract is concentrated and dried to obtain the Cyperus rotundus extract.

2. The seasonal Chinese herbal tea according to claim 1, characterized in that, The spring herbal tea is composed of the following ingredients in the indicated weight proportions: 2.5-3.5 parts chrysanthemum, 1.5-2.5 parts goji berries, 1.5-2.5 parts white peony extract, 1.5-2.5 parts golden lotus, and 1.5-2.5 parts licorice polysaccharide; The summer herbal tea is composed of the following ingredients in the indicated weight proportions: 2.5-3.5 parts longan pulp, 2.5-3.5 parts lily bulb, 1.5-2.5 parts bamboo leaf extract, 2.5-3.5 parts poria cocos, and 1.5-2.5 parts licorice polysaccharide; The autumn herbal tea is composed of the following ingredients in the indicated weight proportions: 2.5-3.5 parts of Ophiopogon japonicus, 1.5-2.5 parts of Citrus reticulata peel, 2.5-3.5 parts of Sterculia lychnophora, 1.5-2.5 parts of Acanthopanax senticosus extract, and 1.5-2.5 parts of Astragalus polysaccharide; The winter herbal tea is composed of the following ingredients in the indicated weight proportions: 4.5-5.5 parts dried ginger, 1.5-2.5 parts Cyperus rotundus extract, 5.5-6.5 parts Polygonatum sibiricum, 4.5-5.5 parts Cornus officinalis, 1.5-2.5 parts ginseng, 9.5-10.5 parts jujube, and 1.5-2.5 parts licorice polysaccharide.

3. The seasonal Chinese herbal tea according to claim 2, characterized in that, The spring herbal tea is composed of the following ingredients in the indicated weight proportions: 3 parts chrysanthemum, 2 parts wolfberry, 2 parts white peony extract, 2 parts golden lotus, and 2 parts licorice polysaccharide. The summer herbal tea is composed of the following ingredients in the indicated weight proportions: 3 parts longan pulp, 3 parts lily bulb, 2 parts bamboo leaf extract, 3 parts poria cocos, and 2 parts licorice polysaccharide. The autumn herbal tea is composed of the following ingredients in the indicated weight proportions: 3 parts Ophiopogon japonicus, 2 parts Citrus reticulata peel, 3 parts Sterculia lychnophora, 2 parts Acanthopanax senticosus extract, and 2 parts Astragalus polysaccharide. The winter herbal tea is composed of the following ingredients in the indicated weight proportions: 5 parts dried ginger, 2 parts Cyperus rotundus extract, 6 parts Polygonatum sibiricum, 5 parts Cornus officinalis, 2 parts ginseng, 10 parts jujube, and 2 parts licorice polysaccharide.

4. A method for preparing a traditional Chinese medicine tea suitable for all four seasons, characterized in that, Specifically, the following operations are included: Weigh each ingredient of the spring herbal tea in any one of claims 1 to 3 according to the weight ratio, crush and mix them, and place them in a tea bag to obtain the spring herbal tea. Weigh each ingredient of the summer medicinal tea in any one of claims 1 to 3 according to the weight ratio, crush and mix them, and place them in a tea bag to obtain the summer medicinal tea. Weigh each ingredient of the autumn medicinal tea in any one of claims 1 to 3 according to the weight ratio, crush and mix them, and place them in a tea bag to obtain the autumn medicinal tea. Weigh each ingredient of the winter medicinal tea in any one of claims 1 to 3 according to the weight ratio, crush and mix them, and place them in a tea bag to obtain the winter medicinal tea. By combining and packaging the spring herbal tea, summer herbal tea, autumn herbal tea, and winter herbal tea, the four-season Chinese herbal tea is obtained.

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