A method for constructing a s. cerevisiae for producing squalene

By co-locating FPPS and SQS enzymes in Saccharomyces cerevisiae, replacing the HMG1 gene with tHMG1, knocking out OPI3 and CHO2, and expressing the PLIN3 gene, the problems of low catalytic efficiency and insufficient storage in squalene production by Saccharomyces cerevisiae were solved, achieving efficient squalene production and storage.

CN116463230BActive Publication Date: 2026-07-10WANHUA CHEM GRP CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
WANHUA CHEM GRP CO LTD
Filing Date
2022-01-11
Publication Date
2026-07-10

AI Technical Summary

Technical Problem

Existing brewing yeast methods for producing squalene suffer from problems such as low enzyme catalytic efficiency, insufficient squalene storage capacity, and high cytotoxicity.

Method used

By genetically modifying farnesyl pyrophosphate synthase (FPPS) and squalene synthase (SQS) to co-localize them to the surface of lipid droplets, replacing the HMG1 gene with a truncated tHMG1, knocking out the OPI3 and CHO2 genes, and expressing the exogenous perilipin 3 gene PLIN3, the squalene storage capacity of Saccharomyces cerevisiae was improved.

Benefits of technology

It significantly increased the squalene production of Saccharin yeast by 6 times, reaching 0.78 g/L, and reduced its toxicity to host cells.

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Abstract

The application discloses a construction method of saccharomyces cerevisiae for producing squalene. The recombinant saccharomyces cerevisiae disclosed by the application co-locates farnesyl pyrophosphate synthetase (FPPS) and squalene synthetase (SQS) on the surface of a lipid droplet, replaces an HMG1 gene with a truncated tHMG1 gene, knocks out OPI3 and CHO2 genes, and expresses an exogenous PLIN3 gene. The application co-locates the key enzymes FPPS and SQS for synthesizing squalene on the surface of a lipid droplet of saccharomyces cerevisiae by using a molecular localization technology, shortens the spatial distance of the enzymes involved in continuous catalysis, stores squalene by using a lipid droplet, reduces the toxicity of squalene to a host cell, and further adjusts the size and quantity of the lipid droplet, so that the yield of squalene is increased by 6 times.
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