Application of LuPDH-E1 beta 1 gene in regulating plant fatty acid synthesis and salt and drought tolerance

By overexpressing the LuPDH-E1β1 gene in plants, the problem of unclear PDH-E1β1 function was solved, and the effects of improving fatty acid synthesis and salt and drought resistance were achieved, filling the gap in the application of genetic engineering and having important breeding significance.

CN116479036BActive Publication Date: 2026-07-24NORTHWEST A & F UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
NORTHWEST A & F UNIV
Filing Date
2023-04-06
Publication Date
2026-07-24

AI Technical Summary

Technical Problem

In the prior art, the function of the β subunit of the E1 component of the plastosome pyruvate dehydrogenase complex (PDH-E1β1) is unclear, which limits its application in genetic engineering.

Method used

By overexpressing the LuPDH-E1β1 gene in plants, we can regulate plant fatty acid synthesis and salt and drought tolerance, thereby increasing the amount of fatty acid synthesis and salt and drought tolerance in plants.

Benefits of technology

Overexpression of the LuPDH-E1β1 gene can increase the amount of fatty acid synthesis in plants, improve the fatty acid content of flax, enhance the salt and drought resistance of plants, elucidate the molecular regulatory mechanism of enzyme activity in seed oil metabolism, and cultivate new flax varieties with high resistance.

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Abstract

The application provides application of a LuPDH-E1beta1 gene in regulating plant fatty acid synthesis, and after overexpression of the LuPDH-E1beta1 gene in plants, the amount of plant fatty acid synthesis can be increased. The application also provides application of the LuPDH-E1beta1 gene in regulating plant salt tolerance and drought resistance, and after overexpression of the LuPDH-E1beta1 gene in plants, the salt tolerance and drought resistance of a PDH-E1beta1 gene deletion plant can be increased. Through exploration of the function of the LuPDH-E1beta1 gene in plant seed oil metabolism and response to abiotic stress, the application of the LuPDH-E1beta1 gene in regulating plant fatty acid synthesis and salt tolerance and drought resistance is given, and a blank in the application of the LuPDH-E1beta1 gene in genetic engineering is filled.
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