Sulfur autotrophic denitrification active microbial carrier and its application

Abstract

The present invention belongs to the field of pollution control technology and discloses a sulfur autotrophic denitrification active microbial carrier and its application. The microbial carrier of the present invention comprises 45 parts of sulfur powder, 15 parts of calcium carbonate, 10-30 parts of polybutylene succinate (PBS), 25 parts of water glass, 5 parts of sodium bicarbonate, and 5 parts of an autotrophic denitrifying agent. The autotrophic denitrifying agent is a mixture of two self-selected bacteria, wherein SZG-SAD-001 has the deposit number: CCTCC NO: M 2022806, and SZG-SAD-002 has the deposit number: CCTCC NO: M 2022805. A high molecular weight slow-release carbon source is added to the carrier to stably release COD, thereby combining autotrophic and heterotrophic denitrification for synergistic denitrification. Simultaneously adding the Mizu no Kuni autotrophic denitrifying agent allows for rapid startup in anaerobic / anoxic environments, eliminating the need for the long acclimatization period required for inoculated sludge, and achieving stable denitrification efficiency.

Description

Technical Field

[0001] The invention belongs to the technical field of pollution control, and in particular relates to a sulfur autotrophic denitrification active microbial carrier and application thereof. Background Art

[0002] Nitrate removal from water is a redox process. Heterotrophic denitrifying bacteria require organic carbon as a carbon source during the nitrate conversion process. However, when organic carbon sources are scarce in wastewater, organic compounds such as methanol or simple organic matter must be added to the wastewater to complete denitrification, and the dosage is difficult to control. Current research on this issue, both domestically and internationally, focuses on methods that utilize elemental sulfur to reduce nitrate. This method can be used under anoxic or anaerobic conditions using elemental sulfur as an electron donor and nitrate as an electron acceptor. Sulfur-autotrophic denitrifying bacteria are particularly advantageous in treating nitrate-containing wastewater because they do not require an external carbon source, thus avoiding secondary pollution, and produce less sludge, thus reducing operating costs, allowing for deep denitrification.

[0003] At present, most sulfur autotrophic denitrification in China mainly uses sulfur powder or pyrite, limestone, siderite, etc. to make carriers or fillers under high-temperature melting conditions. There are many related reports and patents. The application of slow-release carbon sources in sulfur autotrophic carriers has also been studied. The existing slow-release carbon sources are generally divided into three categories: natural materials based on cellulose, artificially synthesized biodegradable polymer materials, and new materials obtained by modifying natural materials. Cellulose is a relatively early material used as a carbon source. Its sources are wide, such as licorice, reed, sugarcane bagasse, corn cobs, straw, rice husks, raw cotton, sawdust, and rotten wood [1][2]. [CN 111362405 B] invented a material for autotrophic and heterotrophic synergistic biological denitrification by using agricultural and forestry organic matter through a special heat treatment process. As for artificially synthesized biodegradable polymers, polyesters and polyhydrocarbons are currently the most studied [3][4]. Their molecular chains contain ester groups, amide groups, and other groups that are easily decomposed by microorganisms, which are used by microorganisms for denitrification. Li Peng et al. [5] conducted a study on denitrification and denitrification using polyhydroxyalkanoates (PHAs). New materials obtained by modifying natural materials, such as starch, chitin and other natural materials, can be used to synthesize solid carbon source materials for use in denitrification water treatment. Currently, the most research is on solid carbon sources made by modifying starch [6][7]. Zhang DY et al. [8] used a blend of starch and polyvinyl alcohol (PVA) as a solid slow-release carbon source for denitrification experiments. Artificially synthesized biodegradable polymers are hydrophobic, and the amount of carbon released during the denitrification process can be well controlled, but they are relatively expensive.

[0004] In order to solve the problems of pH reduction, limited denitrification efficiency, insufficient alkalinity, etc. during sulfur autotrophic deep denitrification, it can be combined with a high molecular slow-release carbon source to provide nutrition for heterotrophic denitrifying microorganisms, so as to achieve autotrophic and heterotrophic synergistic denitrification to make up for their respective deficiencies and improve denitrification efficiency.

[0005] Various components have a great influence on the activity of denitrifying bacteria. At present, there is no active biological carrier product in China that can directly combine denitrifying bacteria with carriers without further treatment. Therefore, based on the above background, it is necessary to develop sulfur autotrophic denitrification active biological carriers for deep denitrification of tailwater treatment, which has important practical significance.

[0006] [1] Wang Jia, Li Xue, Pan Tao, et al. Analysis of recycled water reuse strategies in Beijing [J]. Water and Wastewater, 2013. 39: 208-213

[0007] [2] Shao Liu, Xu Zuxin, Jin Wei, et al. Optimization of solid carbon sources for denitrification of agricultural wastes [J]. China Environmental Science, 2011, 31(5): 748-754.

[0008] [3] Su Tong, Fan Zheng. Removal of nitrate from groundwater using polyhydroxyalkanoates as solid carbon source [J]. Northern Environment, 2011, (6): 138-141.

[0009] [4]Zhiqiang Shen,Yuexi Zhou,Jun Hu,et al.WangbDenitrificationperformance and microbial diversity in a packed-bed bioreactor using biodegradable polymer as carbon source and biofilm support[J].Journal ofHazardous Materials,2013,431-438.

[0010] [5] Li Peng, Tang Lei, Zuo Jian'e, et al. Study on deep denitrification of urban secondary effluent using PHAs as solid carbon source [J]. China Environmental Science, 2014, 34(2): 331-336.

[0011] [6] Li P., Zuo JE, Xing W., et al. Starch / polyvinyl alcohol blended materials used as solid carbon source fortertiary denitrification ofsecondary effluent. Journal of Environmental Sciences, 2013, 25(10): 1972-1979.

[0012] [7] Shen Zhiqiang, Wu Weizhong, Yang Chunping, et al. Study on using starch-based blends as solid carbon sources for denitrification [J]. Environmental Science, 2012, 30(5): 1609-1613.

[0013] [8]Zhang DY, Zhang Summary of the Invention

[0014] The present invention aims to provide a sulfur autotrophic denitrifying active microbial carrier, which contains the self-screened sulfur autotrophic denitrifying bacteria SZG-SAD-001 and SZG-SAD-002, and also contains a slow-release carbon source as an electron donor, without the need for additional nutrients and bacteria, to convert NO3- - N is reduced to N2 and removed.

[0015] Another object of the present invention is to provide an application of a sulfur autotrophic denitrification active microbial carrier in sewage treatment.

[0016] In order to achieve the above object, the present invention adopts the following technical measures:

[0017] A sulfur autotrophic denitrifying active microorganism carrier comprises the following components, in parts by weight:

[0018] 45 parts of sulfur powder, 15 parts of calcium carbonate, 10-30 parts of polybutylene succinate (PBS), 25 parts of water glass, 5 parts of sodium bicarbonate, and 5 parts of autotrophic denitrifying bacteria agent; the autotrophic denitrifying bacteria agent is prepared by mixing SZG-SAD-001 and SZG-SAD-002 in a volume ratio of 2:8 to 10:0, and the effective bacterial concentration of the two bacteria is the same. After addition, the effective bacterial concentration in every 100 grams of sulfur autotrophic denitrifying active microbial carrier is 1*10 8 -10 9 CFU, the deposit number of SZG-SAD-001 is: CCTCC NO: M 2022806, the deposit number of SZG-SAD-002 is: CCTCC NO: M 2022805, and the autotrophic denitrifying bacterial agent is a liquid bacterial agent.

[0019] The microbial carrier described above is characterized in that the volume ratio of SZG-SAD-001 and SZG-SAD-002 is 2:8 to 8:2, with the optimal ratio being 8:2.

[0020] The above-mentioned microbial carrier is characterized in that the effective bacteria concentration in every 100 grams of sulfur autotrophic denitrification active microbial carrier is 5*10 8 CFU.

[0021] The protection scope of the present invention also includes: application of the above-mentioned sulfur autotrophic denitrification active microbial carrier in sewage treatment.

[0022] The use of the above-mentioned sulfur autotrophic denitrifying active microbial carrier in reducing the nitrate or nitrite content in water has the following advantages compared with the prior art:

[0023] In existing technologies, sulfur autotrophic carriers are primarily processed through high-temperature melt granulation. Sulfur melts above 120°C, generating sulfur vapor, which impacts the environment. Furthermore, sulfur is flammable and explosive, posing a certain risk. Granulation at room temperature can avoid this problem. High-temperature melt granulation of conventional sulfur carriers affects nutrient dissolution, making long-term operation unreliable. Adding a high-molecular-weight slow-release carbon source to the carrier steadily releases COD, combining autotrophic and heterotrophic denitrification for synergistic denitrification. Simultaneously incorporating an autotrophic denitrifying bacterial agent from the Water Kingdom allows for rapid startup in anaerobic / anoxic environments, eliminating the need for the long acclimatization period required for inoculated sludge and ensuring stable denitrification efficiency.

[0024] The present invention independently screened two sulfur autotrophic denitrifying bacteria. Compared with other existing denitrifying bacteria, the bacterial agent provided by the present invention is more suitable for use with a carrier, thereby achieving a better nitrogen removal effect. DETAILED DESCRIPTION

[0025] The present invention is further illustrated below with reference to several examples. The experimental methods used in the following examples are conventional methods unless otherwise specified. Materials and reagents used in the following examples are commercially available unless otherwise specified. The sulfur powder used in the examples has a sulfur content of no less than 90%, and all materials are ground to a 100-mesh size.

[0026] The autotrophic denitrification liquid bacterial agent of the present invention is Castellaniella denitrificansi SZG-SAD-002 (preservation number: CCTCC NO: M 2022805) and Sulfurimonas sp. SZG-SAD-001 (preservation number: CCTCC NO: M 2022806), and both bacteria have sulfur autotrophic denitrification function.

[0027] Example 1:

[0028] Obtaining sulfur autotrophic denitrifying bacteria SZG-SAD-001 and SZG-SAD-002

[0029] Fresh sludge from the anaerobic system of a pig farm was taken, and suspensions of different samples were prepared respectively. The suspensions were inoculated into enrichment culture medium at a ratio of 5% v / v. The composition of the culture medium was 0.5g NH4Cl, 0.4g MgSO4·7H2O, 2g KH2PO4, 5g Na2S2O3·5H2O, 2g KNO3, 1g NaHCO3, and 0.01g FeSO4·7H2O. The volume was made up to 1L with distilled water, and the pH was adjusted to 7.0. The suspensions were sealed and cultured in a constant temperature incubator at 28°C. The culture conditions were observed every day, and ammonia nitrogen, nitrate, nitrite, total nitrogen, and pH were measured every four days. After TN dropped to a certain level and did not change, the culture was continued for 4 to 6 times.

[0030] A solid selective culture medium for Thiobacillus denitrificans is prepared by adding 2% agar powder to a liquid culture medium formula, streaking a plate with the final enriched liquid culture medium, allowing single colonies to grow on the plate, selecting the best-growing colonies for culture and then streaking again for isolation, repeatedly isolating and screening until microscopic observation confirms that pure strains of the same morphology are obtained. The pure strains are inoculated onto slant culture medium and stored at 4°C for future use. The selected strains are then identified. Finally, two strains with sulfur autotrophic denitrification capabilities were isolated: Salfurimonas sp. SZG-SAD-001 (Accession No.: CCTCC NO: M 2022806) and Castellaniella denitrificansi SZG-SAD-002 (Accession No.: CCTCC NO: M 2022805).

[0031] Salfurimonas sp. SZG-SAD-001 was deposited in the China Center for Type Culture Collection on June 7, 2022, with the classification name: Salfurimonas sp. SZG-SAD-001, deposit number: CCTCC NO: M 2022806, address: Wuhan University, Wuhan, China. In the present invention, the strain is referred to as SZG-SAD-001.

[0032] Castellaniella denitrificansi SZG-SAD-002 was deposited in the China Center for Type Culture Collection on June 7, 2022, with the classification name: Castellaniella denitrificansi SZG-SAD-002, deposit number: CCTCC NO: M 2022805, address: Wuhan University, Wuhan, China. In the present invention, this strain is referred to as SZG-SAD-002.

[0033] The two strains were inoculated into the culture medium at an inoculum volume of 10% v / v. The pH of the culture medium was adjusted to 7.0-7.2, sterilized at 121°C for 30 min, and cultured at 30°C for 24 h. The concentration of the culture medium of SZG-SAD-001 was greater than or equal to 10 8 CFU / ml, the concentration in the culture medium of SZG-SAD-002 is greater than or equal to 10 8 CFU / ml, and concentrated for use in the following examples.

[0034] The culture medium comprises the following components in parts by weight: 1000 parts of deionized water, 6 parts of sodium thiosulfate pentahydrate, 3 parts of potassium nitrate, 2 parts of sodium bicarbonate, 2 parts of potassium dihydrogen phosphate, 1 part of magnesium chloride hexahydrate, and 0.02 parts of ferrous sulfate heptahydrate.

[0035] Example 2:

[0036] Preparation of sulfur autotrophic denitrification active microbial carrier:

[0037] In this example, the remaining components were the same, and different slow-release carbon sources were changed to determine the optimal slow-release carbon source. All carriers were prepared according to the following method:

[0038] Weigh the materials, mix them evenly, stir and mix them evenly, add appropriate amount of water, add autotrophic denitrifying bacteria agent, shape them, and then crush them into irregular particles with a particle size of 3-8mm. Curing is carried out at room temperature and 100% saturated humidity for more than 15 days.

[0039] In this embodiment, the autotrophic denitrifying agent is made by mixing SZG-SAD-001 and SZG-SAD-002 in a volume ratio of 1:1, and the effective bacterial concentration of the two bacteria is the same. After addition, the effective bacterial concentration in every 100 grams of sulfur autotrophic denitrifying active microbial carrier is 5*10 8 CFU.

[0040] The following parts are all by weight.

[0041] Carrier PLC:

[0042] 45 parts of sulfur powder, 15 parts of calcium carbonate, 15 parts of slow-release carbon source polycaprolactone (PLC), 25 parts of water glass, 5 parts of sodium bicarbonate, and 5 parts of autotrophic denitrifying bacteria agent.

[0043] Carrier PHA:

[0044] 45 parts of sulfur powder, 15 parts of calcium carbonate, 15 parts of slow-release carbon source polyhydroxyalkanoate (PHA), 25 parts of water glass, 5 parts of sodium bicarbonate, and 5 parts of autotrophic denitrifying bacteria agent.

[0045] Carrier PLA:

[0046] 45 parts of sulfur powder, 15 parts of calcium carbonate, 15 parts of slow-release carbon source polylactic acid (PLA), 25 parts of water glass, 5 parts of sodium bicarbonate, and 5 parts of autotrophic denitrifying bacteria agent.

[0047] Carrier PBS:

[0048] 45 parts of sulfur powder, 15 parts of calcium carbonate, 15 parts of slow-release carbon source polybutylene succinate (PBS), 25 parts of water glass, 5 parts of sodium bicarbonate, and 5 parts of autotrophic denitrifying bacteria agent.

[0049] Carrier PHBV:

[0050] 15 parts of calcium carbonate, 15 parts of polyhydroxybutyrate and valerate (PHBV), 25 parts of water glass, 5 parts of sodium bicarbonate, and 5 parts of autotrophic denitrifying bacteria agent.

[0051] Example 3:

[0052] Preparation of carriers with different addition amounts of PBS. The following parts are all by weight.

[0053] Preparation of carrier PBS-1:

[0054] 45 parts of sulfur powder, 15 parts of calcium carbonate, 20 parts of polybutylene succinate (PBS), 25 parts of water glass, 5 parts of sodium bicarbonate, and 5 parts of autotrophic denitrifying bacteria agent.

[0055] Preparation of carrier PBS-2:

[0056] 45 parts of sulfur powder, 15 parts of calcium carbonate, 25 parts of polybutylene succinate (PBS), 25 parts of water glass, 5 parts of sodium bicarbonate, and 5 parts of autotrophic denitrifying bacteria agent.

[0057] Preparation of carrier PBS-3:

[0058] 45 parts of sulfur powder, 15 parts of calcium carbonate, 30 parts of polybutylene succinate (PBS), 25 parts of water glass, 5 parts of sodium bicarbonate, and 5 parts of autotrophic denitrifying bacteria agent.

[0059] Preparation of carrier PBS-4:

[0060] 45 parts of sulfur powder, 15 parts of calcium carbonate, 15 parts of polybutylene succinate (PBS), 25 parts of water glass, 5 parts of sodium bicarbonate, and 5 parts of autotrophic denitrifying bacteria agent.

[0061] Preparation of carrier PBS-5:

[0062] 45 parts of sulfur powder, 15 parts of calcium carbonate, 10 parts of polybutylene succinate (PBS), 25 parts of water glass, 5 parts of sodium bicarbonate, and 5 parts of autotrophic denitrifying bacteria agent.

[0063] The preparation method of this embodiment is the same as that of Example 2. In this embodiment, the autotrophic denitrifying agent is prepared by mixing SZG-SAD-001 and SZG-SAD-002 in a volume ratio of 1:1, and the effective bacterial concentration of the two bacteria is the same. After addition, the effective bacterial concentration in every 100 grams of sulfur autotrophic denitrifying active microbial carrier is 5*10 8 CFU.

[0064] Example 4:

[0065] Preparation of a Sulfur Autotrophic Denitrifying Active Microorganism Carrier Control Group (or simply referred to as a Carrier Control Group in the Examples of the Present Invention)

[0066] Preparation of vehicle control group (using agricultural straw powder as carbon source):

[0067] 45 parts of sulfur powder, 15 parts of calcium carbonate, 15 parts of straw powder, 25 parts of water glass, 5 parts of sodium bicarbonate, and 5 parts of autotrophic denitrifying bacteria agent.

[0068] The preparation method of this embodiment is the same as that of Example 2. In this embodiment, the autotrophic denitrifying agent is prepared by mixing SZG-SAD-001 and SZG-SAD-002 in a volume ratio of 1:1, and the effective bacterial concentration of the two bacteria is the same. After addition, the effective bacterial concentration in every 100 grams of sulfur autotrophic denitrifying active microbial carrier is 5*10 8 CFU.

[0069] Example 5:

[0070] Effect of sulfur autotrophic denitrification active microbial carrier in simulated wastewater:

[0071] The microbial carriers (ie fillers) prepared in Examples 2 to 4 were respectively loaded into column-type water treatment reactors.

[0072] In this embodiment, the filler is loaded into 11 columns in total. The column reactor has a diameter of 8 cm, a loading height of 42 cm, a loading volume of 1.2 L, and a filling rate of about 60 parts.

[0073] Run in the following manner, configure the simulated wastewater KNO30.144g / L, KH2PO40.004g / L, that is, NO3 - It is 20mg / L and TP is 1mg / L.

[0074] Fill the device with simulated wastewater, close the water inlet and outlet, and wait until the nitrate drops by 50% before conducting the water inlet and outlet test, which means normal operation;

[0075] Experimental condition 1: influent total nitrogen concentration 20 mg / L, influent dissolved oxygen concentration 5-6 mg / L, residence time 8 h.

[0076] Experimental condition 2: influent total nitrogen concentration 20 mg / L, influent dissolved oxygen concentration 5-6 mg / L, residence time 4 h.

[0077] Experimental condition 3: influent total nitrogen concentration 20 mg / L, influent dissolved oxygen concentration 5-6 mg / L, residence time 2 h.

[0078] Experimental condition 4: influent total nitrogen concentration 20 mg / L, influent dissolved oxygen concentration 5-6 mg / L, residence time 1 h.

[0079] The operating results under different experimental conditions are shown in the following table:

[0080] Table 1 Operational results of different embodiments under different experimental conditions (unit nitrate residual mg / L)

[0081]

[0082]

[0083] From the above experimental results, we can know that:

[0084] 1. In the present invention, the components are uniformly stirred and then granulated to obtain a stable sulfur autotrophic denitrification biologically active carrier material, especially a high molecular weight slow-release carbon source PBS carrier material. Under the same residence time, the residual nitrate concentration is lower and the denitrification rate is higher.

[0085] 2. According to the results in Table 1, the removal effects of carriers PBS-1 to PBS-5 were better than those of other microbial carriers. Among them, carrier PBS-4 had the best effect in removing nitrate. When the residence time was ≥4h, no nitrate remained, achieving a deep denitrification effect.

[0086] Example 6:

[0087] Effects of different ratios of SZG-SAD-001 and SZG-SAD-002 on the start-up time of sulfur autotrophic denitrification

[0088] Based on the component formula of carrier PBS-4, the ratio of SZG-SAD-001 to SZG-SAD-002 in its autotrophic denitrifying bacteria was changed. The volume ratios of SZG-SAD-001 and SZG-SAD-002 fermentation broth were 0:10, 2:8, 5:5, 8:2, and 10:0, respectively. The remaining components and ratios were the same as those of carrier PBS-4. The prepared microbial carriers were loaded into column-type water treatment reactors.

[0089] In this embodiment, the filler is loaded into 5 columns in total. The column reactor has a diameter of 8 cm, a loading height of 42 cm, a loading volume of 1.2 L, and a filling rate of about 60 parts.

[0090] Configure simulated wastewater KNO30.144g / L, KH2PO40.004g / L, that is, NO3 - is 20mg / L and TP is 1mg / L.

[0091] Fill the device with simulated wastewater, close the water inlet and outlet, and test the time required for nitrate to drop to 50% under different ratios of the two bacteria, that is, the start-up time.

[0092] As shown in Table 2, when the fermentation liquid volume ratio is 8:2, the startup is fastest, the nitrate removal effect is best, and there is no salt accumulation.

[0093] Table 2 Startup results of SZG-SAD-001 and SZG-SAD-002 fermentation broth at different volume ratios

[0094]

[0095] Therefore, the optimal ratio of microbial carrier is:

[0096] 45 parts of sulfur powder, 15 parts of calcium carbonate, 15 parts of polybutylene succinate (PBS), 25 parts of water glass, 5 parts of sodium bicarbonate, and 5 parts of autotrophic denitrifying bacteria agent. The volume ratio of SZG-SAD-001 and SZG-SAD-002 is 8:2, and the effective bacterial concentration of the two bacteria is the same. After addition, the effective bacterial concentration in every 100 grams of sulfur autotrophic denitrifying active microbial carrier is 5*10 8 CFU, used in the following examples.

[0097] Example 7:

[0098] Comparison of the effects of autotrophic denitrifying bacteria prepared by combining SZG-SAD-001 and SZG-SAD-002 with other denitrifying bacteria

[0099] The optimal microbial carrier prepared in Example 6 was loaded into a column-type water treatment reactor.

[0100] The control group consisted of BioPower110Plus composite denitrifying bacteria and BioPower112Plus denitrifying bacteria (products of Wuhan Shuizhiguo Environmental Protection Technology Co., Ltd.) with denitrification ability. That is, the autotrophic denitrifying bacteria in the optimal microbial carrier were replaced with BioPower110Plus composite denitrifying bacteria or BioPower112Plus denitrifying bacteria, respectively, and the effective bacterial concentration in the carrier was controlled at the same time. The weight of the bacterial agent was the same as that in the optimal microbial carrier.

[0101] In this embodiment, the filler is loaded into three columns in total. The column reactor has a diameter of 8 cm, a loading height of 42 cm, a loading volume of 1.2 L, and a filling rate of about 60 parts.

[0102] Run in the following manner, configure the simulated wastewater KNO30.144g / L, KH2PO40.004g / L, that is, NO3 - The concentration of nitrate was 20 mg / L, and the concentration of TP was 1 mg / L. A 50% decrease in nitrate was considered a successful culture. The inlet and outlet water HRT was set to 4 hours. After stable operation, the effluent data was recorded as shown in Table 3. This indicates that even with the same denitrification capacity, the composite bacterial agent of the present invention, combined with a microbial carrier composed of other components, achieved a faster startup speed and better nitrate and nitrite removal rates.

[0103] Table 3 Comparison of SZG-SAD-001: SZG-SAD-002, BioPower110Plus, and BioPower112Plus

[0104]

[0105] Example 8:

[0106] Effects of different ratios of SZG-SAD-001 and BioPower110Plus on nitrate removal

[0107] The autotrophic denitrifying bacteria in the optimal microbial carrier were replaced with SZG-SAD-001 and BioPower110Plus, and the effective bacterial concentration in the carrier was controlled. The weight of the bacteria was the same as that in the optimal microbial carrier, so that the effective bacterial concentration in every 100 grams of the prepared microbial carrier was 5*10 8 CFU.

[0108] The volume ratios of SZG-SAD-001 and BioPower110Plus fermentation broth were 0:10, 2:8, 5:5, 8:2, and 10:0, respectively. The effective bacterial concentrations of SZG-SAD-001 and BioPower110Plus were the same.

[0109] In this embodiment, the filler is loaded into 5 columns in total. The column reactor has a diameter of 8 cm, a loading height of 42 cm, a loading volume of 1.2 L, and a filling rate of about 60 parts.

[0110] Run in the following manner, configure the simulated wastewater KNO30.144g / L, KH2PO40.004g / L, that is, NO3 - The concentration of nitrate was 20 mg / L, and the TP was 1 mg / L. A 50% decrease in nitric acid was considered a successful culture. The inlet and outlet HRT was set at 4 hours. After stable operation, the effluent data was recorded as shown in Table 4. It can be seen that the combination of the autotrophic denitrifying bacteria SZG-SAD-001 and BioPower110Plus was less effective, with nitrite accumulation, when using the carrier PBS-4 (45 parts sulfur powder, 20 parts calcium carbonate, 15 parts slow-release carbon source polybutylene succinate (PBS), 25 parts water glass, and 5 parts sodium bicarbonate).

[0111] Table 4 Effect of different ratios of SZG-SAD-001 and BioPower110Plus on nitrate removal

[0112]

[0113] The above is only a specific implementation method of the present application. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present application. These improvements and modifications should also be regarded as the scope of protection of the present application.

Claims

1. A sulfur autotrophic denitrifying active microorganism carrier, comprising the following components, in parts by weight: 45 parts of sulfur powder, 15 parts of calcium carbonate, 15 parts of polybutylene succinate, 25 parts of water glass, 5 parts of sodium bicarbonate, and 5 parts of autotrophic denitrifying bacteria agent; The autotrophic denitrifying bacteria agent is Sulfurimonas sp. SZG-SAD-001 and Castellaniella denitrificansi SZG-SAD-002 was mixed in a volume ratio of 8:

2. The effective bacterial concentration of the two bacteria was the same. After addition, the effective bacterial concentration in every 100 grams of sulfur autotrophic denitrification active microbial carrier was 1*10 8 -10 9 CFU; The deposit number of SZG-SAD-001 is CCTCC NO: M 2022806, and the deposit number of SZG-SAD-002 is CCTCC NO: M 2022805; The autotrophic denitrifying bacterial agent is a liquid bacterial agent.

2. The microbial carrier according to claim 1, characterized in that: The effective bacterial concentration in every 100g of sulfur autotrophic denitrification active microbial carrier is 5*10 8 CFU.

3. Use of the microbial carrier according to claim 1 in sewage treatment.

4. Use of the microbial carrier according to claim 1 in reducing the nitrate or nitrite content in water.

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