An experimental setup for a rat and mouse model of radiation-induced bladder injury.

Abstract

This invention provides an experimental apparatus for a rat or mouse radiation-induced bladder injury model, comprising a mounting base, a gas delivery tube, an experimental chamber, and a fixation device. The gas delivery tube is used to deliver anesthetic gas and is rotatably mounted on the mounting base; specifically, the gas delivery tube is longitudinally mounted on the mounting base. The experimental chamber is connected to the gas delivery tube, thus allowing the experimental chamber to rotate on the mounting base along with the gas delivery tube. The experimental chamber is sealable, and its inner cavity communicates with the gas delivery tube. The upper end of the experimental chamber has multiple first holes for X-ray penetration, and the outer periphery of the experimental chamber has multiple openable and closable second holes. This invention allows for the simultaneous construction of bladder injury models in multiple rats or mice, and the fixation of a single rat or mouse only requires one attempt, thus saving considerable time.

Description

Technical Field

[0001] This invention relates to the field of experimental equipment technology, specifically to an experimental apparatus for a rat and mouse model of radiation-induced bladder injury. Background Technology

[0002] In existing technologies, the first step in constructing a radiation-induced bladder injury model in rats or mice is to anesthetize them and then fix them in place to facilitate subsequent shaving of the abdominal area. The second step involves fixing the rats or mice in a supine position and irradiating their pelvic cavity with X-rays. The third step involves observing the rats' or mice's food and water intake, body weight, and survival rate, and studying bladder function through urinary behavior patterns. Constructing a radiation-induced bladder injury model in a single rat or mouse takes several minutes, and typically, the above procedures need to be repeated with more rats or mice in a single experiment, which is quite time-consuming. Summary of the Invention

[0003] To address the shortcomings of existing technologies, this invention provides an experimental apparatus for a rat and mouse model of radiation-induced bladder injury, thereby reducing time wastage.

[0004] This invention provides an experimental device for a rat and mouse radiation-induced bladder injury model, comprising: a mounting base; a gas delivery tube rotatably mounted on the mounting base for delivering anesthetic gas; an experimental chamber connected to the gas delivery tube, the inner cavity of the experimental chamber communicating with the gas delivery tube, the upper end of the experimental chamber having multiple first holes for X-rays to pass through, and the outer periphery of the experimental chamber having multiple openable and closable second holes; and a fixing device, multiple of which are disposed within the experimental chamber for fixing rats or mice, and for transporting rats or mice into and out of the experimental chamber through corresponding second holes, wherein the fixing device can be positioned below a live imaging device when transporting rats or mice outside the experimental chamber.

[0005] Preferably, the fixing device includes: a rotating shaft arranged longitudinally and rotatably disposed within the experimental chamber; a connecting seat connected to the upper end of the rotating shaft; a first locking plate slidably disposed laterally on the connecting seat; two rows of locking rods arranged horizontally, the two rows of locking rods being respectively connected to both sides of the first locking plate; and a second locking plate connected to the connecting seat; the first locking plate, the locking rods, and the second locking plate form a space for locking rats and mice, and two adjacent locking rods form a slit to accommodate the feet of rats or mice.

[0006] Preferably, the connecting seat includes: a first seat body connected to the upper end of the rotating shaft; and a second seat body rotatably connected to the side of the first seat body; the first locking plate is laterally slidably disposed on the second seat body, and the second locking plate is fixedly connected to the second seat body.

[0007] Preferably, the fixing device further includes a support plate, one end of which is slidably disposed on the second base body along the longitudinal direction, and the first locking plate has a third hole extending through the length direction, the third hole being slidably engaged with the support plate, and the first locking plate has a first notch at the end away from the second base body.

[0008] Preferably, the outer wall of the second seat is provided with two parallel locking surfaces. The fixing device further includes: a support rod, arranged longitudinally and connected to the upper end of the first seat; a stop block, connected to the upper end of the support rod; a locking plate, which is sleeved on the support rod through a through hole, and whose lower end can abut against the locking surface of the second seat; and a spring, which is sleeved on the support rod and whose two ends abut against the stop block and the locking plate, respectively.

[0009] Preferably, the fixing device further includes a fixing rod slidably mounted on the locking rod, and there are at least two fixing rods located on both sides of the first locking plate, wherein the center line and sliding direction of the fixing rod are perpendicular to the center line of the locking rod.

[0010] Preferably, the experimental chamber includes: a bottom plate connected to the gas supply pipe; an annular side plate whose lower end is connected to the bottom plate and has a second hole thereon; and a top plate connected to the upper end of the annular side plate and has a first hole thereon; the rotating shaft is rotatably mounted on the bottom plate.

[0011] Preferably, it further includes: a first arc-shaped plate, which is slidably installed in the experimental chamber and is arranged in a one-to-one correspondence with the second hole, and has a first guide hole on it and a rack on its inner side wall; and a gear, which is sleeved in a one-to-one correspondence with each of the rotating shafts and meshes with the rack on the first arc-shaped plate.

[0012] Preferably, it further includes: a second arc-shaped plate, which is slidably disposed on the first arc-shaped plate along an arc-shaped trajectory, and has a through second guide hole thereon; and an elastic element disposed on the first arc-shaped plate, so that the second arc-shaped plate tends to be held on the first arc-shaped plate; and a second guide rod is connected to the end of the locking rod away from the first locking plate in the same row.

[0013] Preferably, it further includes: a reference rod, connected to the outer wall of the annular side plate, corresponding one-to-one with the second hole, and located above the second hole; and a partition, connected to the gas supply pipe, of which multiple partitions are provided; a fixing device is provided between two adjacent partitions, the upper end of the gas supply pipe is connected to the top plate, and a through-hole is provided on the side wall of the gas supply pipe corresponding to each fixing device.

[0014] Compared with the prior art, the present invention has the following beneficial effects:

[0015] In this invention, multiple rat or mouse bladder injury models can be constructed simultaneously, and the fixation of a single rat or mouse only needs to be performed once, thus saving a lot of time. Attached Figure Description

[0016] To more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the accompanying drawings used in the description of the specific embodiments or the prior art will be briefly introduced below. In all the drawings, similar elements or parts are generally identified by similar reference numerals. In the drawings, the elements or parts are not necessarily drawn to scale.

[0017] Figure 1 This is a perspective view of an experimental apparatus for a rat and mouse radiation-induced bladder injury model according to an embodiment of the present invention;

[0018] Figure 2 for Figure 1 A three-dimensional view of the experimental chamber;

[0019] Figure 3 for Figure 2 Another 3D image;

[0020] Figure 4 for Figure 2 A three-dimensional view of the fixing device in the middle;

[0021] Figure 5 for Figure 4 Another 3D view (without a second locking plate);

[0022] Figure 6 for Figure 5 Another 3D image;

[0023] Figure 7 for Figure 2 The three-dimensional view at point S (the first and second arc plates cooperate with each other).

[0024] Figure label:

[0025] 10. Mounting base;

[0026] 20. Gas supply pipe; 21. Gas outlet;

[0027] 30. Experimental chamber; 31. First hole; 32. Second hole; 33. Magnet; 34. Handle; 35. Base plate; 36. Annular side plate; 37. Top plate;

[0028] 40. Fixing device; 41. Rotating shaft; 42. Connecting seat; 421. First seat body; 422. Second seat body; 423. Locking surface; 43. First locking plate; 431. First notch; 44. Second locking plate; 45. Locking rod; 451. Fixing rod; 452. Second guide rod; 46. Support plate; 47. Support rod; 471. Spring; 48. Stop block; 49. Locking plate;

[0029] 50. X-ray irradiator;

[0030] 60. Live imaging device;

[0031] 70. First arc-shaped plate; 701. First guide hole; 71. Gear; 72. Second arc-shaped plate; 721. Second guide hole; 73. Elastic element; 74. First guide rod; 75. Guide sleeve;

[0032] 80. Reference rod; 81. Partition. Detailed Implementation

[0033] The embodiments of the technical solution of the present invention will now be described in detail with reference to the accompanying drawings. These embodiments are merely illustrative of the technical solution of the present invention and are therefore intended to limit the scope of protection of the present invention.

[0034] It should be noted that, unless otherwise stated, the technical or scientific terms used in this application should have the ordinary meaning as understood by one of ordinary skill in the art to which this invention pertains.

[0035] In the description of this application, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc., indicating the orientation or positional relationship are based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and are not intended to indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of the present invention.

[0036] Furthermore, the terms "first," "second," etc., are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. In the description of this invention, "a plurality of" means two or more, unless otherwise explicitly defined.

[0037] In this application, unless otherwise expressly specified and limited, the terms "installation," "connection," "linking," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this invention according to the specific circumstances.

[0038] In this application, unless otherwise expressly specified and limited, "above" or "below" the second feature can mean that the first feature is in direct contact with the second feature, or that the first feature is in indirect contact with the second feature through an intermediate medium. Furthermore, "above," "on top of," and "over" the second feature can mean that the first feature is directly above or diagonally above the second feature, or simply that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature can mean that the first feature is directly below or diagonally below the second feature, or simply that the first feature is at a lower horizontal level than the second feature.

[0039] See Figures 1 to 7 This embodiment provides an experimental device for a rat and mouse radiation bladder injury model, including a mounting base 10, an air supply tube 20, an experimental chamber 30, and a fixing device 40.

[0040] The gas delivery tube 20 is used to deliver anesthetic gas. The gas delivery tube 20 is rotatably mounted on the mounting base 10; specifically, it is longitudinally mounted on the mounting base 10. The experimental chamber 30 is connected to the gas delivery tube 20, thus the experimental chamber 30 can rotate on the mounting base 10 along with the gas delivery tube 20. The experimental chamber 30 can be sealed, and its inner cavity communicates with the gas delivery tube 20. Multiple first holes 31 for X-rays to pass through are provided at the upper end of the experimental chamber 30. Specifically, a sealing glass is installed inside each first hole 31. Multiple openable and closable second holes 32 are provided on the outer periphery of the experimental chamber 30. Multiple fixing devices 40 are provided inside the experimental chamber 30. These fixing devices 40 are used to fix rats or mice and can transport them into and out of the experimental chamber 30 through the corresponding second holes 32. When transporting rats or mice outside the experimental chamber 30, they can be positioned below the live imaging device 60. The X-ray irradiator 50, which emits X-rays, can be set up independently or mounted on the mounting base 10, located above the experimental chamber 30, and emits X-rays onto the experimental chamber 30. The live imaging device 60 can be set up independently or mounted on the mounting base 10, located on one side above the experimental chamber 30.

[0041] In this embodiment, multiple rat or mouse bladder injury models are constructed simultaneously. First, near-infrared small molecule dyes are instilled into the rats or mice. Each rat or mouse is then secured using a different fixation device 40 and transported to the experimental chamber 30. Then, anesthetic gas is introduced into the experimental chamber 30 via a gas inlet tube 20 to simultaneously anesthetize multiple rats or mice. The fixation device 40 then transports the rats or mice out of the experimental chamber 30 through the second hole 32. After shaving the abdominal hair of the rats or mice, the bladder position of the rats or mice below is determined using a live imaging device 60. Since the experimental chamber 30 is rotatable, only one live imaging device 60 is needed. After determining the bladder position of the rats or mice using the live imaging device 60, the position of the rats or mice on the fixation device 40 is finely adjusted. When the fixation device 40 transports the rats or mice into the experimental chamber 30, the bladder position of the rats or mice is precisely below the first hole 31. X-rays pass through the first hole 31 and directly strike the bladder of the rats or mice, causing damage. Traditional rat or mouse radiation-induced bladder injury models require anesthesia, fixation, shaving, transfer to an X-ray irradiator 50, fixation again, continuous anesthesia, and radiation irradiation. This process must be repeated multiple times if multiple rats or mice are being tested. Our device, however, only requires a single fixation of the rat or mouse using the fixation device 40, saving time. Furthermore, it allows for simultaneous anesthesia and X-ray irradiation of multiple rats or mice, saving only a fraction of the time compared to traditional methods. For example, if four fixation devices 40 are used, simultaneously fixing four rats or mice would reduce the time required to approximately one-quarter of the traditional method. In addition, perfusing near-infrared small molecules into the rats or mice, followed by using a live imaging device 60 to pinpoint the bladder location, and then precise X-ray irradiation, avoids damage to other tissues in the pelvic cavity, thus improving experimental accuracy. The size of the experimental chamber can be customized for rats or mice with varying body sizes.

[0042] In one embodiment, the fixing device 40 includes: a rotating shaft 41, a connecting seat 42, a first locking plate 43, a locking rod 45, and a second locking plate 44.

[0043] The rotating shaft 41 is arranged longitudinally and rotatably installed inside the experimental chamber 30. The connecting seat 42 is connected to the upper end of the rotating shaft 41. The first locking plate 43 is slidably installed on the connecting seat 42 laterally. Two rows of locking rods 45 are arranged horizontally, and the two rows of locking rods 45 are respectively connected to the two sides of the first locking plate 43, with the locking rods 45 in the same row being parallel to each other. The second locking plate 44 is connected to the connecting seat 42. The first locking plate 43, the locking rods 45 and the second locking plate 44 form a space for locking rats or mice, and two adjacent locking rods 45 form a slit to accommodate the feet of rats or mice. In addition, the rotating shaft 41 can be connected to an external motor, or it can pass through the bottom of the experimental chamber 30 and connect to a handle 34. A magnet 33 is provided on the lower surface of the outer side of the experimental chamber 30 corresponding to each handle 34. The handle 34 is made of iron. The rotating shaft 41 is driven to rotate by the handle 34, and the rotating shaft 41 is locked by the magnet 33.

[0044] In this embodiment, as the rotating shaft 41 rotates, the first locking plate 43, locking rod 45, and second locking plate 44 located inside the experimental chamber 30 rotate out of the experimental chamber 30 around the rotating shaft 41, passing through the opened second hole 32. The first locking plate 43 and second locking plate 44 are initially arranged vertically. During operation, the first locking plate 43 is first slid out, and a rat or mouse is placed on it, with its limbs positioned between the locking rods on both sides. Then, the first locking plate 43 is pushed back above or below the second locking plate 44, preventing the rat or mouse from getting up and thus securing it. The operation is simple and quick. The rat or mouse's abdomen can usually be suspended outside the first locking plate 43 to facilitate subsequent shaving and in vivo imaging to locate the bladder. Furthermore, the first locking plate 43 is usually positioned above the second locking plate 44, with the locking rod 45 tilted downwards, so that the rat or mouse is in a supine position when secured, eliminating the need to turn it over later. After the first locking plate 43 and the second locking plate 44 are rotated by the pivot 41, they are placed in the experimental chamber 30. The rats or mice are located in the middle of the experimental chamber 30. Multiple rats or mice are located in the middle of the experimental chamber 30, and the distance between them is small, so that the coverage area of ​​the X-rays above can be adjusted to be very small, thereby saving energy and making the setup reasonable.

[0045] In one embodiment, the connector 42 includes a first seat body 421 and a second seat body 422.

[0046] The first seat 421 is connected to the upper end of the rotating shaft 41, and the second seat 422 is rotatably connected to the side of the first seat 421. Specifically, a hole is opened on one side of the first seat 421, and a coaxial bearing is installed in the hole. A connecting rod is connected to the side of the second seat 422, and the connecting rod is connected to the inner ring of the bearing in the first seat 421.

[0047] The first locking plate 43 is slidably disposed on the second seat 422, and the second locking plate 44 is fixedly connected to the second seat 422.

[0048] In this embodiment, the second seat 422 can rotate relative to the first seat 421, thereby allowing the vertical positions of the first locking plate 43 and the second locking plate 44 to be changed. During operation, the experimenter typically holds the rat or mouse by its back. Placing the rat or mouse in a supine position on the first locking plate 43 is inconvenient. Therefore, by rotating the second seat 422, the first locking plate 43 is positioned below the second locking plate 44 (e.g., ...). Figure 4 Then, pull out the first locking plate 43 and place the rat or mouse upright on it. The rat or mouse's limbs are naturally restrained by the locking rods. Then, push the first locking plate 43 under the second locking plate 44, preventing the rat or mouse from getting up and thus fixing it in place. Rotate the shaft 41, and the rat or mouse enters the experimental chamber 30 through the second hole 32 and is anesthetized by the anesthetic gas output from the gas delivery tube 20. After anesthetizing the rat or mouse, rotate it out of the experimental chamber 30 and rotate the second seat 422 so that the rat or mouse is in a supine position. Then, administer air to it and perform subsequent hair removal and bladder positioning operations. It should be noted that at this time, the first locking plate 43 and the second locking plate 44 are still partially inside the experimental chamber 30, as are the rat or mouse's head, thus keeping the rat or mouse in a stable anesthetized state. This is quite convenient for operators who are not skilled in anesthesia.

[0049] In one embodiment, the fixing device 40 further includes a support plate 46, one end of which is slidably mounted on the second base 422 along the longitudinal direction. Specifically, a groove is formed in the second base 422, and guide rails are respectively provided on the inner walls of the groove along the longitudinal direction on both sides. The two ends of the support plate 46 are respectively connected to the guide rails on the same side. A third hole is formed in the first locking plate 43 along the length direction, and the third hole is slidably engaged with the support plate 46. A first notch 431 is formed at the end of the first locking plate 43 away from the second base 422. In addition, the support plate 46 and the first locking plate 43 can be directly locked by structure, for example, by forming a through hole on one side of the first locking plate 43 and providing a screw for threaded connection with the hole on the first locking plate 43. The screw can abut against the support plate 46, that is, tightening the screw can lock the support plate 46 and the first locking plate 43.

[0050] In this embodiment, the first locking plate 43 slides on the support plate 46 through the third hole, thereby approaching or moving away from the second seat 422. The abdomen of the rat or mouse is located precisely at the first notch 431, which eliminates the need to suspend the abdomen of the rat or mouse when placing it, making the setup more reasonable. The support plate 46 can slide up and down on the second seat 422. Before anesthesia, the rat or mouse is placed upright on the first locking plate 43. After anesthesia, the second seat 422 is rotated 180 degrees, and the rat or mouse is supported by the second locking plate 44. The first seat 421 slides and presses against the rat or mouse on the second locking plate 44, thereby simply fixing the rat or mouse and preventing it from shaking during anesthesia, which would affect subsequent hair removal and bladder positioning.

[0051] In one embodiment, the outer wall of the second seat 422 is provided with two parallel locking surfaces 423. Specifically, the cross-section of the second seat 422 is a circle. The fixing device 40 also includes a support rod 47, a stop block 48, a locking plate 49, and a spring 471.

[0052] The support rod 47 is arranged longitudinally and connected to the upper end of the first seat 421. The stop block 48 is connected to the upper end of the support rod 47. The locking plate 49 is sleeved on the support rod 47 through a through hole, and the lower end of the locking plate 49 abuts against the locking surface 423 of the second seat 422. The spring 471 is sleeved on the support rod 47, and the two ends of the spring 471 abut against the stop block 48 and the locking plate 49 respectively.

[0053] In this embodiment, the rotation of the second seat 422 can change the posture of the rat or mouse. To ensure the stable posture of the rat or mouse for subsequent bladder positioning and X-ray irradiation, a locking plate 49 is abutted against the outer wall of the second seat 422. Preferably, the second seat 422 is cylindrical in shape, with a plane (locking surface 423) on each of its two outer walls. When the locking surface 423 on the second seat 422 abuts against the locking plate 49, the second seat 422 will not easily rotate, thereby keeping the first locking plate 43 and the second locking plate 44 in their original positions, so that the rat or mouse on it can be stably in a supine or prone position.

[0054] In one embodiment, the fixing device 40 further includes a fixing rod 451 slidably mounted on the locking rod 45. There are at least two fixing rods 451, which are located on both sides of the first locking plate 43. Preferably, there are four fixing rods 451, corresponding to the limbs of a rat or mouse. The center line and sliding direction of the fixing rod 451 are perpendicular to the center line of the locking rod 45.

[0055] In this embodiment, the rat or mouse lies prone on the first locking plate 37, with its limbs positioned between the locking bars 45 on both sides. At the same time, the rat or mouse's limbs are also resting on the fixing bar 451. When the second seat 422 rotates, the rat or mouse assumes a supine position. At this time, the rat or mouse's limbs need to be spread out as much as possible. The fixing bar 451 has a certain weight, and under the gravity of the fixing bar 451, the rat or mouse's limbs are spread out, which facilitates subsequent hair removal, bladder positioning, and X-ray irradiation.

[0056] In one embodiment, the experimental chamber 30 includes a bottom plate 35, an annular side plate 36, and a top plate 37.

[0057] The base plate 35 is connected to the gas supply pipe 20. The lower end of the annular side plate 36 is connected to the base plate 35. A second hole 32 is opened on the upper part of the annular side plate 36. The top plate 37 is connected to the upper end of the annular side plate 36, and a first hole 31 is opened on it. The rotating shaft 41 is rotatably mounted on the base plate 35. The top plate 37 is a lead plate, and X-rays can only irradiate the rats or mice in the experimental chamber 30 through the first hole 31, avoiding damage to other parts of the rats or mice.

[0058] In one embodiment, it further includes a first arcuate plate 70 and a gear 71.

[0059] A first arc-shaped plate 70 is slidably installed inside the experimental chamber 30, corresponding one-to-one with the second hole 32. It has a first guide hole 701 and a rack on its inner sidewall. Specifically, the first arc-shaped plate 70 and the annular side plate 36 have the same curvature. Furthermore, an elastic sealing strip is provided on the outer sidewall of the first arc-shaped plate 70, surrounding the first guide hole 701 and fitting against the inner wall of the annular side plate 36. Gears 71 are sleeved one-to-one with each rotating shaft 41, meshing with the rack on the first arc-shaped plate 70. Specifically, guide rails are connected to both the upper and lower sides of the first arc-shaped plate 70, and these guide rails are connected to the inner wall or bottom of the experimental chamber 30.

[0060] In this embodiment, the rotation of the shaft 41 drives the gear 71 to rotate, which in turn drives the first arc-shaped plate 70 to slide. Initially, the first guide hole 701 and the second hole 32 on the first arc-shaped plate 70 are misaligned. Combined with the effect of the elastic sealing strip, this ensures a good seal in the experimental chamber 30. As the first arc-shaped plate 70 slides, the first guide hole 701 and the second hole 32 partially overlap. Simultaneously, the first locking plate 43 and the second locking plate 44 also protrude through the first guide hole 701 and the second hole 32 outside the experimental chamber 30. The main function of the first arc-shaped plate 70 is to reduce the size of the airflow channel inside and outside the experimental chamber 30, thereby preventing a large amount of anesthetic gas from leaking out and being wasted, while also ensuring a stable and continuous anesthetic effect in rats or mice. This device can be used inside a fume hood. It should be noted that researchers often anesthetize rats or mice using a regular box, and the opening and closing of this box can also cause anesthetic gas leakage. Therefore, a small amount of anesthetic gas leakage is normal.

[0061] In one embodiment, it further includes: a second arcuate plate 72 and an elastic element 73.

[0062] The second arc-shaped plate 72 is slidably mounted on the first arc-shaped plate 70 along an arc-shaped trajectory. It has a through second guide hole 721. The second arc-shaped plate 72 and the first arc-shaped plate 70 have the same curvature, and their sliding trajectories are parallel. Specifically, the first arc-shaped plate 70 has two parallel first guide rods 74. The upper and lower ends of the second arc-shaped plate 72 are respectively connected to guide sleeves 75, which slidably fit on the first guide rods 74 on the same side, allowing the second arc-shaped plate 72 to slide on the first arc-shaped plate 70. An elastic element 73 is provided on the first arc-shaped plate 70, giving the second arc-shaped plate 72 a tendency to remain on the first arc-shaped plate 70. The elastic element 73 preferably consists of four springs 471, which are respectively fitted on the upper and lower first guide rods 74, applying elastic force to the middle second arc-shaped plate 72 from both sides. A second guide rod 452 is connected to the end of the locking rod 45 furthest from the first locking plate 43.

[0063] In this embodiment, when the rotating shaft 41 rotates, the first locking plate 43 and the second locking plate 44 rotate outside the experimental chamber 30. During this process, the first arc-shaped plate 70 rotates to the second hole 32, and the first guide hole 701 gradually coincides with the second hole 32. The first guide hole 701 is smaller than the second hole 32. In order to further reduce the channel size for airflow inside and outside the experimental chamber 30, the second arc-shaped plate 72 can adapt to the position of the first locking plate 43 and the second locking plate 44 under the action of the spring 471. That is, when the first locking plate 43 and the second locking plate 44 rotate out from the second guide hole 721, the second guide rod 452 on the locking rod 45 will inevitably abut against the inner wall of the second guide hole 721. The second arc-shaped block can slide to make way, thereby allowing the size of the second guide hole 721 to be designed to be smaller, thereby reducing the airflow inside and outside the experimental chamber 30, avoiding the leakage of a large amount of anesthetic gas, and reducing waste.

[0064] In one embodiment, a reference rod 80 and a partition 81 are also included.

[0065] Reference rod 80 is connected to the outer wall of annular side plate 36, and reference rod 80 is set one-to-one with second hole 32, located above second hole 32. Partition plate 81 is connected to air supply pipe 20, and multiple partition plates are provided. A fixing device 40 is set between two adjacent partition plates 81. The upper end of air supply pipe 20 is connected to top plate 37, and a through air outlet 21 is opened on the side wall of air supply pipe 20 corresponding to each fixing device 40.

[0066] In this embodiment, after anesthetizing the rat or mouse, it rotates out of the experimental chamber 30, and the second seat 422 is rotated so that the rat or mouse is in a supine position. After shaving the fur on the abdomen of the rat or mouse through the first notch 431, the in vivo imaging device 60 is turned on. It can be seen that the near-infrared small molecule aggregation area is the bladder of the rat or mouse. By adjusting the position of the first locking plate 43 on the support plate 46 and by manually moving the rat or mouse slightly laterally on the first locking plate 43, the bladder of the rat or mouse is positioned below the end of the reference rod 80. It can be seen that the bladder of the rat or mouse is completely blocked by the end of the reference rod 80. Subsequently, the rotating shaft 41 is rotated so that after the rat or mouse enters the experimental chamber 30, the bladder of the rat or mouse is directly opposite the first hole 31. This eliminates the need for manual marking and improves efficiency.

[0067] Numerous specific details are set forth in this specification. However, it will be understood that embodiments of the invention may be practiced without these specific details. In some instances, well-known methods, structures, and techniques have not been shown in detail so as not to obscure the understanding of this specification.

[0068] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some or all of the technical features therein. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention, and they should all be covered within the scope of the claims and specification of the present invention.

Claims

1. An experimental apparatus for a rat and mouse model of radiation-induced bladder injury, characterized in that, include: Mounting bracket (10); The gas delivery tube (20) is rotatably mounted on the mounting base (10) and is used to deliver anesthetic gas; An experimental chamber (30) is connected to the gas supply pipe (20). The inner cavity of the experimental chamber (30) is connected to the gas supply pipe (20). The upper end of the experimental chamber (30) has multiple first holes (31) for X-rays to pass through. The outer periphery of the experimental chamber (30) has multiple openable and closable second holes (32). The fixation device (40) is provided in multiple locations within the experimental chamber (30) for fixing rats or mice. It can transport rats or mice into and out of the experimental chamber (30) through the corresponding second hole (32). When the fixation device (40) transports rats or mice outside the experimental chamber (30), it can be located below the live imaging device (60). The fixing device (40) includes: A rotating shaft (41) is arranged longitudinally and rotatably disposed within the experimental chamber (30); The connecting seat (42) is connected to the upper end of the rotating shaft (41); The first locking plate (43) is slidably disposed on the connecting seat (42); The locking levers (45) are arranged in two rows, with both rows of locking levers (45) at the same level, and the two rows of locking levers (45) are respectively connected to both sides of the first locking plate (43); and The second locking plate (44) is connected to the connecting seat (42); The first locking plate (43), the locking rod (45) and the second locking plate (44) form a space for locking a rat or mouse, and two adjacent locking rods (45) form a slit to accommodate the feet of the rat or mouse.

2. The experimental apparatus for a rat / mouse radiation-induced bladder injury model as described in claim 1, characterized in that, The connector (42) includes: The first seat (421) is connected to the upper end of the rotating shaft (41); and The second seat (422) is rotatably connected to the side of the first seat (421); The first locking plate (43) is slidably disposed on the second seat (422), and the second locking plate (44) is fixedly connected to the second seat (422).

3. The experimental apparatus for a rat / mouse radiation-induced bladder injury model as described in claim 2, characterized in that, The fixing device (40) further includes a support plate (46), one end of which is slidably disposed on the second seat (422) along the longitudinal direction. The first locking plate (43) has a third hole extending through the length direction, which is slidably engaged with the support plate (46). The first locking plate (43) has a first notch (431) at the end away from the second seat (422).

4. The experimental apparatus for a rat / mouse radiation-induced bladder injury model as described in claim 3, characterized in that, The outer wall of the second base (422) is provided with two parallel locking surfaces (423), and the fixing device (40) further includes: The support rod (47) is arranged longitudinally and connected to the upper end of the first base (421); The stop block (48) is connected to the upper end of the support rod (47); A locking plate (49) is fitted onto the support rod (47) through a through hole, and its lower end abuts against the locking surface (423) of the second seat (422); and Spring (471) is sleeved on the support rod (47), and its two ends abut against the stop block (48) and the locking plate (49) respectively.

5. The experimental apparatus for a rat / mouse radiation-induced bladder injury model as described in any one of claims 2-4, characterized in that, The fixing device (40) further includes a fixing rod (451) slidably mounted on the locking rod (45). There are at least two fixing rods (451) and they are located on both sides of the first locking plate (43). The center line and sliding direction of the fixing rod (451) are perpendicular to the center line of the locking rod (45).

6. The experimental apparatus for a rat / mouse radiation-induced bladder injury model as described in claim 5, characterized in that, The experimental chamber (30) includes: The base plate (35) is connected to the gas pipeline (20); An annular side plate (36), the lower end of which is connected to the bottom plate (35), and the second hole (32) is formed thereon; and The top plate (37) is connected to the upper end of the annular side plate (36), and the first hole (31) is opened on it. The rotating shaft (41) is rotatably mounted on the base plate (35).

7. The experimental apparatus for a rat and mouse radiation-induced bladder injury model as described in claim 6, characterized in that, Also includes: A first arc-shaped plate (70) is slidably installed inside the experimental chamber (30), corresponding one-to-one with the second hole (32), and has a first guide hole (701) on it, with a rack on its inner sidewall; and The gear (71) is sleeved one-to-one with each of the said shafts (41) and meshes with the rack on the first arc plate (70).

8. The experimental apparatus for a rat and mouse radiation-induced bladder injury model as described in claim 7, characterized in that, Also includes: A second arc-shaped plate (72) is slidably disposed on the first arc-shaped plate (70) along an arc-shaped trajectory, and a through second guide hole (721) is provided thereon; and An elastic element (73) is provided on the first arc-shaped plate (70) to give the second arc-shaped plate (72) a tendency to remain on the first arc-shaped plate (70); A second guide rod (452) is connected to the end of the locking rod (45) that is away from the first locking plate (43).

9. The experimental apparatus for a rat and mouse radiation-induced bladder injury model as described in claim 8, characterized in that, Also includes: Reference rod (80), connected to the outer wall of the annular side plate (36), is configured to correspond one-to-one with the second hole (32), and is located above the second hole (32); and A partition (81) is connected to the gas transmission pipe (20), and multiple partitions are provided; A fixing device (40) is set between two adjacent partitions (81). The upper end of the gas pipe (20) is connected to the top plate (37). A through-hole (21) is opened on the side wall of the gas pipe (20) corresponding to each fixing device (40).

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