Methods for effectively regulating lily bulb dormancy

Abstract

The present invention discloses several methods for regulating and controlling the release of lily bulb dormancy, belonging to the field of plant cultivation technology. The dormancy release time is effectively regulated by chemical reagent treatment: 2,3-butanedione monoxime (BDM) and 2-deoxy-D-glucose (DDG) treatment can shorten the dormancy time; N-ethylmaleimide (NEM) treatment can prolong the dormancy time. The method comprises the following steps: (1) selecting bulbs: washing the bulbs and airing them until the surface is dry to obtain dormant bulbs; (2) chemical treatment of the bulbs: punching holes in the base of the bulbs described in (1), immersing them in NEM, BDM, and DDG reagents respectively, and performing chemical treatment in combination with a vacuum pump; (3) storing and cultivating the bulbs: embedding the treated bulbs described in (2) in a matrix, placing them in a cold storage for storage, and then planting them. The present invention can regulate the storage period of lily bulbs by treating them with different chemical reagents according to market demand. The technology is simple, practical, low-cost, and easy to promote.

Description

Technical Field

[0001] The invention belongs to the technical field of plant cultivation, and relates to the field of releasing bulb dormancy, and in particular to several methods for effectively regulating lily bulb dormancy. Background Art

[0002] Lily, a perennial herbaceous plant of the genus Lilium in the family Liliaceae, is one of the four major cultivated fresh cut flowers in China. Lily bulbs exhibit a natural dormancy, the release of which directly impacts developmental processes such as primordium formation, flower bud differentiation, flowering regulation, and peduncle elongation. Therefore, precise control of lily bulb dormancy and research into key storage technologies for lily production are crucial factors in achieving forced cultivation and year-round supply of lily cut flowers.

[0003] Existing methods for shortening the dormancy period of green lilies involve treating with warm water, drying, soaking in thiadipyrrolidone, and placing them in full sunlight. However, these methods involve multiple steps and are complex. Furthermore, methods that shorten the dormancy period of lily bulbs by treating them at low temperatures for extended periods of time are time-consuming and fail to address the actual production and market demands for delaying the release of dormancy. Therefore, finding a method that can easily and quickly regulate lily bulb dormancy and extend or shorten its storage period is urgent. Summary of the Invention

[0004] (1) Technical issues to be solved

[0005] The present invention aims to provide several effective methods for regulating lily dormancy. Lily bulbs were treated with different chemical reagents. After low-temperature storage, it was observed that bulbs treated with 2,3-butanedione 2-monoxime (BDM) and 2-deoxy-D-glucose (DDG) were able to germinate earlier, breaking dormancy and achieving early dormancy release in the lily cultivar 'Siberia'. Furthermore, bulbs treated with N-ethylmaleimide (NEM) showed delayed germination, achieving delayed dormancy release in the lily cultivar 'Siberia'.

[0006] (2) Technical solution

[0007] In a first aspect, the present invention provides several methods for effectively regulating lily bulb dormancy, the methods comprising the following steps:

[0008] (1) Selection of bulbs: Wash the bulbs and air dry them until the surface is dry to obtain dormant bulbs;

[0009] (2) Bulb treatment: Punch holes at the base of the dormant bulbs described in (1), immerse them in NEM, BDM, and DDG reagents respectively, and use a vacuum pump to pump them to a negative pressure state;

[0010] (3) Storage and cultivation of bulbs: The bulbs treated in (2) are embedded in a matrix and placed in a cold storage for storage before planting.

[0011] In the present invention, treatment with NEM, BDM, and DDG chemical reagents can affect the blockage and unclogging of plasmodesmata in lily bulb buds, thereby affecting the transport of nutrients during bulb dormancy. This treatment triggers a series of physiological and biochemical phenomena during dormancy release in lily bulbs, affecting bud germination and growth, thereby regulating the dormancy period of lily bulbs.

[0012] In some preferred embodiments, the drying site is an open area, preferably a solar greenhouse.

[0013] In some preferred embodiments, the puncturing of the base of the bulb is performed using a syringe needle, the puncturing position is the base of the bulb, and the number of punctures is 10 to 15.

[0014] In some preferred embodiments, when the vacuum pump is used to evacuate to negative pressure, the atmospheric pressure in the pump is 0.8 MPa, the treatment time is 15 minutes, and then the air is slowly released for 15 minutes until the atmospheric pressure in the pump reaches the standard atmospheric pressure.

[0015] In some preferred embodiments, the concentration of NEMBDM and DDG is 1 mmol / L.

[0016] In some preferred embodiments, the culture matrix is a mixed matrix of matrix: sawdust = 1:1, and the storage conditions are as follows: temperature: 4°C, duration: 4 to 8 weeks.

[0017] In some preferred embodiments, the diameter of the bulb to be processed is 6 to 7 centimeters.

[0018] In some preferred embodiments, the planting conditions are as follows: temperature: 22 to 27°C, 16 hours of light, and 8 hours of darkness.

[0019] In some preferred embodiments, the lily is the Oriental lily 'Siberia' variety, which has a deep dormancy level and generally requires low temperature treatment at 4°C for 8 weeks to break dormancy.

[0020] (3) Beneficial effects

[0021] In the present invention, treatment with NEM, BDM, and DDG chemical reagents can affect the blockage and unclogging of plasmodesmata in the apical meristem of lily bulbs, thereby affecting the transport of nutrients during bulb dormancy and regulating lily bulb dormancy. This method can advance or delay the release of bulb dormancy based on market demand and cultivation conditions, effectively improving land resource utilization and boosting lily production efficiency. The present method is simple and practical, effectively regulating lily bulb dormancy, while being low-cost, easy to manage, and suitable for large-scale promotion. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for describing the embodiments or the prior art.

[0023] Figure 1 Shows the growth of lily variety 'Siberia' after being treated with different reagents;

[0024] Figure 2 The figure shows the statistics of bud length / bulb length of lily variety 'Siberia' bulbs after treatment with different reagents;

[0025] Figure 3 Shows the germination rate (%) of bulbs of lily variety 'Siberia' after treatment with different reagents;

[0026] Figure 4 Shows the height (cm) of lily cultivar 'Siberia' plants after treatment with different reagents;

[0027] Figure 5 Schematic diagram showing different treatment methods for lily bulbs. DETAILED DESCRIPTION

[0028] The present invention discloses several methods for effectively regulating lily bulb dormancy. Those skilled in the art can refer to the contents of this article and appropriately improve the process parameters to achieve the desired results. It should be noted that all similar substitutions and modifications are obvious to those skilled in the art and are considered to be included in the present invention. The methods and applications of the present invention have been described through preferred embodiments. It is obvious that relevant personnel can modify or appropriately change and combine the methods and applications described herein without departing from the content, spirit and scope of the present invention to implement and apply the technology of the present invention.

[0029] As described above, the present invention provides several methods for quickly relieving lily bulb dormancy in a first aspect, the methods comprising the following steps:

[0030] (1) Selection of bulbs: Wash the bulbs and air dry them until the surface is dry to obtain dormant bulbs;

[0031] (2) Chemical treatment of bulbs: pierce the base of the dormant bulbs described in (1), immerse them in NEM, BDM, and DDG chemical reagents, and pump them to a negative pressure state using a vacuum pump;

[0032] (3) Storage and cultivation of bulbs: The bulbs treated in (2) are embedded in a culture medium and stored in a cold storage before planting.

[0033] The present invention will be further described below in conjunction with the embodiments:

[0034] Example 1 NEM treatment of bulbs

[0035] (1) Obtaining bulbs: For the lily variety 'Siberia', harvest mature dormant bulbs after the aboveground part withers.

[0036] (2) Bulb treatment (NEM treatment): Three groups of bulbs were selected and the experiment was repeated three times. Nine bulbs of uniform size, approximately 6 to 7 cm in diameter, were washed and punctured with a syringe needle at the base of the stem 10 to 15 times. The bulbs were then placed in a vacuum pump and filled with 1 mmol / L NEM solution until the bulbs were completely submerged. The vacuum pump was then tightly closed and the pressure inside the pump was reduced to 0.8 MPa and maintained at this level for 15 minutes. The air was then slowly released over 15 minutes to allow the pressure inside the pump to slowly return to standard atmospheric pressure.

[0037] (3) Storage and planting of bulbs: After drying the treated bulbs, embed them in a culture medium with a matrix: sawdust = 1:1, keep the culture medium moist, store them in a cold storage at 4℃ for 6 weeks, and then plant them in a solar greenhouse for cultivation.

[0038] (4) Regularly observe and count the growth, germination and later plant growth of the bulb buds. The experimental results are shown in Tables 1-4 for Experimental Examples 1-3 and Figure 1-4 shown.

[0039] Example 2 BDM treatment of bulbs

[0040] (1) Obtaining bulbs: For the lily variety 'Siberia', harvest mature dormant bulbs after the aboveground part withers.

[0041] (2) Bulb treatment (BDM treatment): Three groups of bulbs were selected and the experiment was repeated three times. Each group consisted of 9 bulbs of uniform size, approximately 6 to 7 cm in diameter. After washing, 10 to 15 holes were pierced in the base of the stem using a syringe needle. The bulbs were then placed in a vacuum pump and injected with 1 mmol / L BDM until they were completely submerged. The vacuum pump was then tightly closed and the pressure inside the pump was reduced to 0.8 MPa and maintained at this level for 15 minutes. The air was then slowly released over 15 minutes to allow the pressure inside the pump to slowly return to standard atmospheric pressure.

[0042] (3) Storage and planting of bulbs: After drying the treated bulbs, embed them in a culture medium with a matrix: sawdust = 1:1, keep the culture medium moist, store them in a cold storage at 4℃ for 4 weeks, and then plant them in a solar greenhouse for cultivation.

[0043] (4) Regularly observe and count the growth, germination and later plant growth of the bulb buds. The experimental results are shown in Tables 1-4, Experimental Examples 4-6 and Figure 1-4 shown.

[0044] Example 3 DDG treatment of bulbs

[0045] (1) Obtaining bulbs: For the lily variety 'Siberia', harvest mature dormant bulbs after the aboveground part withers.

[0046] (2) Bulb treatment (DDG treatment): Three groups of bulbs were selected and the experiment was repeated three times. Each group consisted of nine bulbs of uniform size, approximately 6 to 7 cm in diameter. After washing, 10 to 15 holes were punctured in the base of the stem using a syringe needle. The bulbs were then placed in a vacuum pump and filled with 1 mmol / L DDG solution until the bulbs were completely submerged. The vacuum pump was then tightly capped and the pressure inside the pump was reduced to 0.8 MPa and maintained at this level for 15 minutes. The air was then slowly released over 15 minutes to allow the pressure inside the pump to slowly return to standard atmospheric pressure.

[0047] (3) Storage and planting of bulbs: After drying the treated bulbs, embed them in a culture medium with a matrix: sawdust = 1:1, keep the culture medium moist, store them in a cold storage at 4℃ for 6 weeks, and then plant them in a solar greenhouse for cultivation.

[0048] (4) Regularly observe and count the growth, germination and later plant growth of the bulb buds. The experimental results are shown in Tables 1-4, Experimental Examples 7-9 and Figure 1-4 shown.

[0049] Comparative example water treatment bulbs

[0050] (1) Obtaining bulbs: For the lily variety 'Siberia', harvest mature dormant bulbs after the aboveground part withers.

[0051] (2) Bulb treatment (water treatment): Three groups of bulbs were selected and the experiment was repeated three times. Each group consisted of 9 bulbs of uniform size, about 6 to 7 cm in diameter. After washing, 10 to 15 holes were pierced in the base of the stem using a syringe needle. The bulbs were then placed in a vacuum pump and filled with clean water until the bulbs were completely submerged. The vacuum pump was then tightly closed and the pressure inside the pump was reduced to 0.8 MPa and maintained at this level for 15 minutes. The air was then slowly released over 15 minutes to allow the pressure inside the pump to slowly return to standard atmospheric pressure.

[0052] (3) Storage and planting of bulbs: After drying the treated bulbs, embed them in a culture medium with a matrix: sawdust = 1:1, keep the culture medium moist, store them in a cold storage at 4℃ for 6 weeks, and then plant them in a solar greenhouse for cultivation.

[0053] (4) Regularly observe and count the growth, germination and later plant growth of the bulb buds. The experimental results are shown in Tables 1-4 for Comparative Examples 1-3 and Figure 1-4 shown.

[0054] Effect Example 1 Statistics and analysis of the growth of lily variety "Siberia" bulbs treated with different reagents before planting

[0055] In Examples 1 to 3 and the comparative example, three bulbs were randomly selected from each group and cut open to observe and count the growth of the bulbs. The results are shown in Table 1 below:

[0056] Table 1. Statistics and analysis of the growth of lily variety "Siberia" bulbs before planting treated with different reagents (bud length / bulb length)

[0057]

[0058] Note: Bud length / bulb length is used as the standard to measure the growth rate of buds. The larger the bud length / bulb length value, the faster the bud grows, and the smaller the bud length / bulb length value, the slower the bud grows.

[0059] Effect Example 2 Statistics on the growth and germination of bulbs of lily variety "Siberia" treated with different reagents

[0060] The remaining 6 bulbs in each group of Examples 1 to 3 and the comparative example were planted and their growth was observed and counted. The results are shown in Tables 2 to 4 below:

[0061] Table 2. Statistics of germination of lily variety "Siberia" bulbs treated with different reagents 4 weeks after planting

[0062]

[0063]

[0064] Note: "0" indicates no germination, "1" indicates germination; bulbs with buds larger than 1.00 cm are considered to have germinated, while bulbs with buds smaller than 1.00 cm are considered to have not germinated.

[0065] Table 3. Growth statistics of lily variety "Siberia" bulbs treated with different reagents 8 weeks after planting (cm)

[0066]

[0067] Table 4. Analysis of bulb growth of lily variety "Siberia" treated with different reagents

[0068]

[0069]

[0070] In summary, the present invention can regulate the release of bulb dormancy by treating lily bulbs with different chemical reagents, effectively and quickly release or prolong the bulb dormancy time, which is of great significance for lilies to adapt to the market and improve market production efficiency.

[0071] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as within the scope of protection of the present invention.

Claims

1. A method for effectively regulating the release of lily bulb dormancy, characterized in that: The method comprises the following steps: (1) Selection of bulbs: Wash the bulbs and air dry them until the surface is dry to obtain dormant bulbs; (2) Treatment of bulbs: Punch holes at the base of the dormant bulbs described in (1), immerse them in 1mmol / L NEM, 1mmol / L BDM, or 1mmol / L DDG reagent, respectively, and use a vacuum pump to pump the bulbs to a negative pressure state. The bulbs treated with BDM or DDG reagent germinate early, thereby achieving early dormancy release of the bulbs, while the bulbs treated with NEM reagent germinate later, thereby achieving delayed dormancy release of the bulbs. (3) Storage and cultivation of bulbs: The bulbs treated in (2) are embedded in a matrix and placed in a cold storage for storage before planting.

2. The method according to claim 1, characterized in that In the step (1), the drying area is an open area.

3. The method according to claim 1, characterized in that In the step (2), the base of the bulb is punctured using a syringe needle, the puncture position is the base of the bulb, and the number of puncture holes is 10 to 15.

4. The method according to claim 1, wherein In the step (2), when the vacuum pump is used to evacuate to negative pressure, the atmospheric pressure in the pump is 0.8 MPa, the processing time is 15 minutes, and then the air is slowly released for 15 minutes until the atmospheric pressure in the pump reaches the standard atmospheric pressure.

5. The method according to any one of claims 1 to 4, characterized in that In step (2), the diameter of the bulbs to be processed is 6 to 7 centimeters.

6. The method according to any one of claims 1 to 5, characterized in that In the step (3), the culture medium is a mixed medium of matrix: sawdust = 1:1, and the storage conditions are as follows: temperature: 4°C, duration: 4 to 8 weeks.

7. The method according to any one of claims 1 to 6, characterized in that In step (3), the planting conditions are as follows: temperature: 22 to 27°C, 16 hours of light, and 8 hours of darkness.

8. The method according to any one of claims 1 to 7, characterized in that The lily is of the 'Siberia' variety.

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