A nucleic acid detection assay kit and methods of use thereof

Abstract

The application provides a nucleic acid detection analysis kit and a use method thereof, and relates to the technical field of biological detection, and comprises a central shaft with a central flow channel, a suction module is arranged in the central flow channel, and a groove is further arranged on the central shaft; a main box is arranged around the circumference of the central shaft, and is sequentially provided with a lysis cavity, a combination cavity, a rinsing cavity, a drying cavity, an elution cavity and a distribution channel, the main box is sleeved outside the central shaft and can rotate around the shaft, so that the distribution channel and the cavities are selectively connected with the central flow channel, the top of each cavity is closed in an initial state, and the main box is sealingly connected with the central shaft; and a base is provided with a magnetic suction module for storing magnetic beads in the groove. The application solves the problem that the sample pretreatment is still needed in the prior art, the operation is complex, and cross contamination exists in the sample transfer process, which reduces the detection accuracy of the sample.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of biological detection, in particular to a nucleic acid detection analysis kit and a use method thereof. BACKGROUND

[0002] Nucleic acid detection is widely used in disease detection, biological analysis, biological medicine and other fields. The detection process includes sampling of samples, sample pretreatment (sample lysis, washing, drying, elution), nucleic acid extraction, reagent preparation, amplification, etc. In the nucleic acid detection process, the pretreated sample is transferred to the PCR reaction tube for detection.

[0003] At present, the above steps of nucleic acid detection are mainly manually operated, or part of the process is completed with the help of semi-automatic instruments, but there is still a need for sample pretreatment, the operation is complex, and there is a cross contamination risk in the sample transfer process, which will reduce the precision of sample detection. SUMMARY

[0004] In view of the problems in the prior art, the first object of the present application is to provide a nucleic acid detection analysis kit, which solves the problem that the precision of sample detection is reduced due to the need for sample pretreatment, complex operation and cross contamination risk in the sample transfer process in the prior art.

[0005] According to an embodiment of the present application, a nucleic acid detection analysis kit comprises:

[0006] A central shaft having a central flow channel, wherein a suction module is arranged in the central flow channel, and a groove is further arranged on the central shaft;

[0007] A main box, wherein a lysis cavity, a combination cavity, a rinsing cavity, a drying cavity, an elution cavity and a distribution channel are sequentially arranged around the circumference of the central shaft, the main box is sleeved outside the central shaft and can rotate around the shaft, so that the distribution channel and each cavity are selectively connected with the central flow channel, and the top of each cavity is closed in the initial state, and the main box is sealingly connected with the central shaft;

[0008] And a base, wherein a magnetic suction module is arranged to receive magnetic beads into the groove.

[0009] Compared with the prior art, the present application has the following advantages:

[0010] In one aspect, since the kit integrates nucleic acid extraction, reagent preparation and the like in one kit, without sample pretreatment, when using, the sample is directly injected into the lysis cavity, and by gradually rotating the main box, the sample pretreatment, nucleic acid extraction, reagent preparation, amplification and the like can be realized, realizing sample in and result out, and molecular full-automatic process, which is convenient to operate; on the other hand, the whole detection process is in a closed kit, which prevents multiple cross contamination and reagent leakage, avoids the risk of nucleic acid aerosol, sample and reagent leakage.

[0011] Further, the center of the main box is provided with a vertical through mounting hole, and the bottom of the main box is provided with a platform;

[0012] The center shaft comprises:

[0013] A shaft body in a hollow cylindrical shape, the shaft body passes through the mounting hole;

[0014] And a pressure plate fixed on the bottom of the shaft body, the top end surface of the pressure plate is attached to the bottom end surface of the platform, and a sealing gasket is pressed therebetween, a radial channel is provided on the pressure plate and communicates with the inner cavity of the shaft body, the other end of the radial channel is selectively communicated with the distribution channel and each cavity, and a vertical through gas permeation hole is provided on the top end of the pressure plate.

[0015] Further, the bottom of the main box is fixed with a drainage column for clamping a detection tube, and the distribution channel comprises:

[0016] A reaction liquid flow channel, one end of which is communicated with the center flow channel, and the other end of the reaction liquid flow channel is communicated with the drainage column;

[0017] And a gas permeation channel, one end of which is communicated with the drainage column, and the other end of the gas permeation channel is communicated with the gas permeation hole when one end of the reaction liquid flow channel is communicated with the center flow channel.

[0018] Further, the main box has a sink, the distribution channel is provided with a plurality of distribution channels and is arranged on the sink, and the top of each distribution channel is open and sealed by a film.

[0019] Further, the radial channel is provided on the bottom end surface of the pressure plate, and further comprises a center pressure plate fixed on the bottom end surface of the pressure plate and used to seal the radial channel.

[0020] Further, the pressure plate is provided with a clamping groove, the base is in a cylindrical shape and is sleeved outside the main box, and a blocking strip fixed on the base is clamped in the clamping groove.

[0021] Further, the top end of the shaft body protrudes out of the main box and is provided with a clamping hole, and further comprises a gland provided with a buckle clamped in the clamping hole.

[0022] Further, the main box is further provided with a ventilation hole, and the central flow channel is communicated with the ventilation hole in an initial state.

[0023] Further, the main box is further provided with a ventilation hole, and the central flow channel is communicated with the ventilation hole in an initial state.

[0024] In view of the deficiencies in the prior art, a second object of the present application is to provide a use method of a nucleic acid detection analysis kit, which solves the problem of reduced sample detection accuracy due to the need for sample pretreatment, complex operation and cross-contamination risk in the sample transfer process in the prior art.

[0025] According to an embodiment of the present application, a use method of a nucleic acid detection analysis kit uses a nucleic acid detection analysis kit for detection operation, and the base is further provided with a vibration module for mixing and a heating module for heating;

[0026] The sample is injected into the lysis cavity, and after mixing and heating reaction, the magnetic beads in the lysis cavity are adsorbed into the groove;

[0027] Fix the central shaft, rotate the main box clockwise, align the binding cavity with the groove, mix the magnetic beads in the groove, and after the mixing of the magnetic beads in the binding cavity, the magnetic beads are adsorbed into the groove;

[0028] Fix the central shaft, rotate the main box clockwise, align the binding cavity with the groove, mix the magnetic beads in the groove, and after the mixing of the magnetic beads in the binding cavity, the magnetic beads are adsorbed into the groove;

[0029] Fix the central shaft, rotate the main box clockwise, align the binding cavity with the groove, mix the magnetic beads in the groove, and after the mixing of the magnetic beads in the binding cavity, the magnetic beads are adsorbed into the groove;

[0030] Fix the central shaft, rotate the main box clockwise, align the binding cavity with the groove, mix the magnetic beads in the groove, and after the mixing of the magnetic beads in the binding cavity, the magnetic beads are adsorbed into the groove;

[0031] After drying, fix the central shaft, rotate the main box clockwise, align the binding cavity with the groove, mix the magnetic beads in the groove, and after the mixing of the magnetic beads in the binding cavity, the magnetic beads are adsorbed into the groove;

[0032] Fix the central shaft, rotate the main box clockwise, align the binding cavity with the groove, mix the magnetic beads in the groove, and after the mixing of the magnetic beads in the binding cavity, the magnetic beads are adsorbed into the groove;

[0033] Fix the center shaft, rotate the main box clockwise, make one of the distribution flow channels communicate with the center flow channel, inject the eluent in the shaft body cavity into the detection tube through the distribution flow channel by the suction module, and inject the eluent into each detection tube in turn;

[0034] Fix the center shaft, rotate the main box counterclockwise, make the dry cavity communicate with the center flow channel, and inject the excess eluent in the shaft body cavity into the dry cavity through the suction module;

[0035] Fix the center shaft, rotate the main box clockwise, make the paraffin oil cavity communicate with the center flow channel, and suck the paraffin oil into the shaft body cavity through the suction module;

[0036] Fix the center shaft, rotate the main box clockwise, make the distribution channel communicate with the center flow channel, and inject the paraffin oil in the shaft body cavity into the detection tube through the suction module, and inject the paraffin oil into each detection tube in turn;

[0037] Fix the center shaft, rotate the main box clockwise, make the cracking cavity communicate with the center flow channel.

[0038] Compared with the prior art, the present application has the following beneficial effects:

[0039] 1. Suitable for different samples, no sample pretreatment, direct sample addition, simple operation.

[0040] 2. Nucleic acid extraction, reagent configuration, etc. are integrated in one kit, realizing sample in and result out, molecular full automation process, convenient operation;

[0041] 3. The whole detection process is in a sealed kit, preventing multiple cross contamination and reagent leakage, avoiding nucleic acid aerosol, sample and reagent leakage risk. BRIEF DESCRIPTION OF DRAWINGS

[0042] Figure 1 It is a schematic diagram of the overall structure of the embodiment of the present application;

[0043] Figure 2 It is a schematic diagram of the internal structure of Figure 1 ;

[0044] Figure 3 It is a schematic diagram of the center shaft structure in Figure 1 ;

[0045] Figure 4 It is a schematic diagram of the structure under the angle of view from below Figure 3 ;

[0046] Figure 5 It is a schematic diagram of the main box structure in Figure 1 ;

[0047] Figure 6 It is a schematic diagram of the structure under the angle of view from below of Figure 5 ;

[0048] Figure 7 Fig. 1 is a perspective view of the nucleic acid detection analysis kit according to the present application. Figure 5 Fig. 2 is a top view of the nucleic acid detection analysis kit according to the present application.

[0049] In the above figures:

[0050] 101, shaft body; 102, pressure plate; 1021, radial channel; 1022, air vent; 1023, clamping slot; 1024, suction hole; 1025, groove;

[0051] 2, suction module;

[0052] 3, main box; 301, platform; 302, sink; 303, air vent;

[0053] 4, lysis cavity; 5, binding cavity; 6, rinsing cavity; 7, drying cavity; 8, elution cavity;

[0054] 9, liquid separation channel; 901, reaction liquid flow channel; 902, air permeable channel;

[0055] 10, base; 11, sealing gasket; 12, drainage column; 13, detection tube; 14, center tablet; 15, gland; 16, paraffin oil cavity. DETAILED DESCRIPTION

[0056] The technical solutions in the present application will be further described below in combination with the drawings and examples.

[0057] A nucleic acid detection analysis kit, an embodiment of the present application is proposed, as shown in Figure 1 , Figure 2 and Figure 7 , comprising: a central shaft, a main box 3 and a base 10, wherein,

[0058] Specifically refer to Figure 3 and Figure 4, the central shaft has a central flow channel, the suction module 2 is arranged in the central flow channel, and the central shaft is further provided with a groove 1025; for the convenience of understanding, the specific structure of a central shaft is described herein, the central shaft comprises a shaft body 101 and a pressure plate 102, the shaft body 101 is in a hollow cylindrical shape, and the suction module 2, for example, a piston rod, is arranged in the inside of the shaft body 101; the top end surface of the pressure plate 102 is fixedly arranged at the bottom of the shaft body 101, the top end surface of the pressure plate 102 is provided with the downwardly recessed groove 1025 for accommodating the magnetic beads, and the side, away from the shaft body 101, of the pressure plate 102 is provided with a radial channel 1021 which is in communication with the cavity of the shaft body 101, the radial channel 1021 and the cavity of the shaft body 101 together form the central flow channel, the end, away from the shaft body 101, of the radial channel 1021 extends upwardly and penetrates through the pressure plate 102 to form a suction hole 1024, the suction hole 1024 is selectively in communication with the distribution channel 9 and each cavity, and the top end of the pressure plate 102 is provided with a vertical gas permeable hole 1022;

[0059] Specifically refer to Figure 5 and Figure 6 , the main box 3 is in a cylindrical shape, the center of the main box 3 is provided with a vertical installation hole which is matched with the outer diameter of the shaft body 101 in diameter and through which the shaft body 101 penetrates, the bottom of the main box 3 is provided with a platform 301, and the main box 3 is sequentially provided with a lysis cavity 4, a combination cavity 5, a rinsing cavity 6, a drying cavity 7, an elution cavity 8 and a distribution channel 9 around the circumferential direction of the central shaft, of course, the above cavities are previously added with corresponding lysis liquid, rinsing liquid, drying agent, eluent and the like, and the lysis cavity 4 is further added with magnetic beads; wherein, each cavity is vertically penetrated, the distribution channel 9 is provided with a plurality of distribution channels 9, four distribution channels 9 are shown in the drawings of the embodiment, of course, the number is not limited and can be increased or decreased according to actual needs; the main box 3 is sleeved on the central shaft and can rotate around the shaft, so that the distribution channel 9 and each cavity are selectively in communication with the suction hole 1024 of the central flow channel, and the top of each cavity is closed in the initial state (that is, the top of each cavity is closed, and the sample needs to be injected by puncturing the cover film on the top of the lysis cavity 4 during use, which will be described in detail later); the main box 3 is sealingly connected with the central shaft, and in addition, the main box 3 is further provided with a gas vent hole 303, in the initial state, the central flow channel is in communication with the gas vent hole 303, so that the piston in the central shaft can move up and down.

[0060] The base 10 is in a cylindrical shape, the base 10 is provided with a magnetic attraction module, the magnetic attraction module can be an existing electromagnet device, and the magnetic attraction module is located at the bottom of the pressure plate 102 to attract the magnetic beads into the groove 1025 as needed.

[0061] With the above scheme, on the one hand, since the kit integrates nucleic acid extraction and reagent preparation, etc. in one kit, without sample pretreatment, when used, the sample is directly injected into the lysis cavity 4, and the sample pretreatment, nucleic acid extraction, reagent preparation, amplification, etc. can be realized by gradually rotating the main box 3, realizing sample in and result out, and molecular full automation process, which is convenient to operate; on the other hand, the whole detection process is in a closed kit, which prevents multiple cross contamination and reagent leakage, and avoids the risk of nucleic acid aerosol, sample and reagent leakage.

[0062] In another embodiment of the present application, based on the above embodiment, a structure of one of the liquid separation channels 9 is given, as shown in Figure 5 and Figure 6 Specifically, the bottom of the main box 3 is fixedly provided with a cylindrical drainage column 12, the outer diameter of which matches the inner diameter of the detection tube 13 so as to facilitate the clamping of the detection tube 13 on the drainage column 12; the liquid separation channel 9 comprises a reaction liquid flow channel 901 and an air permeation channel 902, wherein,

[0063] One end of the reaction liquid flow channel 901 is on the same circumference as the suction hole 1024, so as to ensure that the main box 3 can be communicated with the central flow channel after rotation, and the other end of the reaction liquid flow channel 901 is communicated with the drainage column 12;

[0064] One end of the air permeation channel 902 is communicated with the drainage column 12, and the other end of the air permeation channel 902 is communicated with the air permeation hole 1022 when one end of the reaction liquid flow channel 901 is communicated with the central flow channel; during liquid separation, the piston rod moves downward, so that the eluent in the shaft body 101 cavity is sequentially injected into the liquid separation tube through the reaction liquid flow channel 901, the drainage column 12 and finally into the liquid separation tube, and at the same time, the air in the liquid separation tube is extruded upward, passes through the air permeation channel 902 and is finally discharged from the air permeation hole 1022, and the liquid separation process is simple and convenient.

[0065] In another embodiment of the present application, based on the above embodiment, the setting mode of the liquid separation channel 9 is further optimized, as shown in Figure 5 Specifically, a part of the main box 3 is recessed downward to form a sunken table 302, and each liquid separation channel 9 is arranged on the sunken table 302, and each liquid separation channel 9 can be formed by being recessed downward, and in practice, a layer of film is covered on the top of the liquid separation channel 9, so as to seal the liquid separation channel 9, which is convenient for forming and manufacturing.

[0066] In another embodiment of the present application, based on the above embodiment, a setting mode of the radial channel 1021 is proposed, as shown in Figure 2 and Figure 4As shown, the radial channel 1021 is formed by concave on the bottom of the pressure disc 102, and the ring side of the pressure disc 102 extends downward, and correspondingly, the center pressing plate 14 is embedded on the bottom of the pressure disc 102 to seal the bottom of the radial channel 1021, so that the liquid only flows along the radial channel 1021 without leakage, and the structure of the radial channel 1021 is simple and convenient to manufacture and process.

[0067] In another embodiment of the present application, on the basis of the above-mentioned embodiment, a connecting mode of the pressure disc 102 and the base 10 is provided, as shown in Figure 1 and Figure 3 As shown, the ring side of the pressure disc 102 is provided with a clamping groove 1023, the base 10 is sleeved on the outer wall of the main box 3, and the base 10 is fixed with a blocking strip clamped in the clamping groove 1023, so that the blocking strip limits the rotation of the pressure disc 102 when the main box 3 rotates, and the center shaft remains stationary; in addition, the inner wall of the base 10 is in contact with the outer wall of the main box 3, which guides and limits the main box 3, and guarantees the coaxiality of the main box 3, the base 10 and the center shaft.

[0068] In another embodiment of the present application, on the basis of the above-mentioned embodiment, a sealing connecting mode of the center shaft and the main box 3 is provided, as shown in Figure 1 and Figure 3 As shown, the top end of the shaft body 101 protrudes from the main box 3 and is provided with a clamping hole, the clamping hole is provided with a plurality of clamping holes and is distributed on the ring side of the shaft body 101, and the inner wall of the gland 15 is provided with a clamping buckle which can be clamped into the clamping hole, the gland 15 is sleeved on the shaft body 101 and is pressed downward, so that the clamping buckle is clamped into the clamping hole to limit the upward and downward movement of the center shaft, and the sealing gasket 11 located between the platform 301 and the pressure disc 102 is guaranteed to be in a compressed state, the structure is simple and convenient to install; of course, the locking mode of the gland 15 and the shaft body 101 is not limited to the above-mentioned mode, for example, the outer wall of the shaft body 101 is provided with external threads, and the inner wall of the gland 15 is provided with internal threads, so as to realize the threaded connection of the gland 15 and the shaft body 101.

[0069] In another embodiment of the present application, on the basis of the above-mentioned embodiment, the structure of the kit is further optimized, as shown in Figure 1 As shown, it further comprises a paraffin oil cavity 16 which vertically penetrates and the bottom of which is connected with the suction hole 1024 of the center flow channel, the paraffin oil cavity 16 and the elution cavity 8 are located on the same circumference, and the paraffin oil cavity 16 is located on the side of the elution cavity 8 away from the drying cavity 7; the cavity is pre-provided with paraffin oil.

[0070] The present application further provides a using method of the nucleic acid detection and analysis kit, which utilizes the above-mentioned nucleic acid detection and analysis kit for detection operation, the base 10 is further provided with an oscillation module (such as an existing ultrasonic oscillator) for mixing and a heating module (also an existing technology, which will not be described here) for heating.

[0071] Pierce the film on the top of the lysis cavity 4, inject the sample into the lysis cavity 4, start the oscillation module and the heating module, after mixing and heating, start the magnetic attraction module to attract the magnetic beads in the lysis cavity 4 into the groove 1025;

[0072] Fix the central shaft, rotate the main box 3 clockwise, so that the combination cavity 5 is aligned with the groove 1025, start the oscillation module to mix the magnetic beads in the groove 1025, after mixing and reacting in the combination cavity 5, start the magnetic attraction module to attract the magnetic beads into the groove 1025;

[0073] Fix the central shaft, rotate the main box 3 clockwise, so that the rinse cavity 6 is aligned with the groove 1025, start the oscillation module to mix the magnetic beads in the groove 1025, after mixing and rinsing in the rinse cavity 6, start the magnetic attraction module to attract the magnetic beads into the groove 1025;

[0074] Fix the central shaft, rotate the main box 3 clockwise, so that the other rinse cavity 6 is aligned with the groove 1025, start the oscillation module to mix the magnetic beads in the groove 1025, after mixing and rinsing in the rinse cavity 6, start the magnetic attraction module to attract the magnetic beads into the groove 1025;

[0075] Fix the central shaft, rotate the main box 3 clockwise, so that the dry cavity 7 is aligned with the groove 1025, after the magnetic beads in the groove 1025 are dried, proceed to the next step;

[0076] After drying, fix the central shaft, rotate the main box 3 clockwise, so that the elution cavity 8 is aligned with the groove 1025, start the oscillation module to mix the magnetic beads in the groove 1025, after mixing and eluting in the elution cavity 8, start the magnetic attraction module to attract the magnetic beads into the groove 1025;

[0077] Fix the central shaft, rotate the main box 3 clockwise, so that the elution cavity 8 is connected to the suction hole 1024 of the central flow channel, pull the piston rod upwards to suck the eluent in the elution cavity 8 into the cavity of the shaft body 101;

[0078] Fix the central shaft, rotate the main box 3 clockwise, so that one of the distribution flow channels is connected to the suction hole 1024 of the central flow channel, push the piston rod downwards to inject the eluent in the cavity of the shaft body 101 into the detection tube 13 through the distribution flow channel (the injection amount is preferably 10-15 microliters), in this embodiment, there are four distribution flow channels, repeat the step to sequentially inject the eluent into each detection tube 13;

[0079] Fix the central shaft, rotate the main box 3 counterclockwise, so that the dry cavity 7 is connected to the suction hole 1024 of the central flow channel, push the piston rod downwards to inject the excess eluent in the cavity of the shaft body 101 into the dry cavity 7.

[0080] Fix the center axis, clockwise rotation of the main box 3, let paraffin oil cavity 16 connected to the suction hole 1024 center flow channel, upwardly pull the piston rod suction paraffin oil to the shaft body 101 cavity;

[0081] Fix the center axis, clockwise rotation of the main box 3, let paraffin oil cavity 16 connected to the suction hole 1024 center flow channel, upwardly pull the piston rod suction paraffin oil to the shaft body 101 cavity;

[0082] Fix the center axis, clockwise rotation of the main box 3, let paraffin oil cavity 16 connected to the suction hole 1024 center flow channel, upwardly pull the piston rod suction paraffin oil to the shaft body 101 cavity;

[0083] Finally, it is pointed out that the above examples are used to illustrate the technical solutions of the present application rather than limit it. Although the present application has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present application can be modified or replaced equivalently without departing from the purpose and scope of the present application, which should be covered in the scope of the claims of the present application.

Claims

1. A nucleic acid detection and analysis kit, characterized in that, include: A central shaft having a central flow channel, wherein a suction module (2) is disposed in the central flow channel, and a groove (1025) is also provided on the central shaft. The main box (3) has a pyrolysis cavity (4), a binding cavity (5), a rinsing cavity (6), a drying cavity (7), an elution cavity (8), and a liquid distribution channel (9) arranged in sequence around the central axis. The main box (3) is fitted outside the central axis and can rotate around the axis so that the liquid distribution channel (9) and each cavity can be connected to the central flow channel. In the initial state, the top of each cavity is sealed and the main box (3) is sealed to the central axis. The base (10) is equipped with a magnetic module for storing the magnetic beads in the groove (1025), and the base (10) is also provided with an oscillation module for mixing and a heating module for heating. The main box (3) has a vertical through mounting hole in the center, and the bottom of the main box (3) has a platform (301). The central axis includes: A shaft (101) is a hollow cylinder, and the shaft (101) passes through the mounting hole; The pressure plate (102) is fixed on the bottom of the shaft (101). The top surface of the pressure plate (102) is in contact with the bottom surface of the platform (301) and a sealing gasket (11) is pressed between them. The pressure plate (102) is provided with a radial channel (1021) that communicates with the inner cavity of the shaft (101). The other end of the radial channel (1021) can be selectively connected to the liquid distribution channel (9) and each cavity. The top of the pressure plate (102) is provided with a vertically penetrating vent hole (1022). The bottom of the main box (3) is fixed with a drainage column (12) for engaging the detection tube (13), and the liquid distribution channel (9) includes: The reaction liquid flow channel (901) has one end connected to the central flow channel and the other end connected to the guide column (12). The ventilation channel (902) is connected at one end to the drainage column (12). When one end of the reaction liquid flow channel (901) is connected to the central flow channel, the other end of the ventilation channel (902) is connected to the ventilation hole (1022).

2. The nucleic acid detection and analysis kit as described in claim 1, characterized in that, The main box (3) has a settling platform (302), and the liquid distribution channel (9) is provided in multiple ways and is set on the settling platform (302). The top of each liquid distribution channel (9) is open and sealed by a membrane.

3. A nucleic acid detection and analysis kit as described in any one of claims 1 to 2, characterized in that, The radial channel (1021) is disposed on the bottom end face of the pressure plate (102), and also includes a central pressure plate (14), which is fixed on the bottom end face of the pressure plate (102) and used to seal the radial channel (1021).

4. A nucleic acid detection and analysis kit as described in any one of claims 1 to 2, characterized in that, The pressure plate (102) is provided with a slot (1023), the base (10) is cylindrical and is sleeved outside the main box (3), and a stop bar is fixed on the base (10) and engaged in the slot (1023).

5. A nucleic acid detection and analysis kit as described in any one of claims 1 to 2, characterized in that, The top end of the shaft (101) extends out of the main box (3) and has a locking hole. It also includes a pressure cap (15) with a buckle that can be inserted into the locking hole.

6. A nucleic acid detection and analysis kit as described in any one of claims 1 to 2, characterized in that, The main box (3) is also provided with a vent (303). In the initial state, the central flow channel is connected to the vent (303).

7. A nucleic acid detection and analysis kit as described in any one of claims 1 to 2, characterized in that, It also includes a paraffin oil cavity (16) which can be connected to the central flow channel. The paraffin oil cavity (16) and the elution cavity (8) are on the same circumference, and the paraffin oil cavity (16) is located on the side of the elution cavity (8) away from the drying cavity (7).

8. A method for using a nucleic acid detection and analysis kit, characterized in that, The detection operation was performed using the nucleic acid detection and analysis kit as described in claim 7; The sample is injected into the pyrolysis cavity (4), and after mixing and heating reaction, the magnetic beads in the pyrolysis cavity (4) are adsorbed into the groove (1025); Fix the central axis and rotate the main box (3) clockwise so that the bonding cavity (5) is aligned with the groove (1025). Mix the magnetic beads in the groove (1025). After the mixed magnetic beads react in the bonding cavity (5), the magnetic beads are then adsorbed into the groove (1025). Fix the central axis and rotate the main box (3) clockwise so that the rinsing cavity (6) is aligned with the groove (1025). Mix the magnetic beads in the groove (1025). After the mixed magnetic beads are rinsed in the rinsing cavity (6), the magnetic beads are attracted into the groove (1025). Fix the central axis and rotate the main box (3) clockwise so that the other rinsing cavity (6) is aligned with the groove (1025). Mix the magnetic beads in the groove (1025). After the mixed magnetic beads are rinsed in the rinsing cavity (6), the magnetic beads are attracted into the groove (1025). Fix the central axis and rotate the main box (3) clockwise to align the drying cavity (7) with the groove (1025) and wait for the magnetic beads in the groove (1025) to dry. After drying, fix the central shaft and rotate the main box (3) clockwise so that the elution chamber (8) is aligned with the groove (1025). Mix the magnetic beads in the groove (1025). After the mixed magnetic beads are eluted in the elution chamber (8), the magnetic beads are adsorbed into the groove (1025). Fix the central shaft and rotate the main box (3) clockwise to connect the elution cavity (8) with the central flow channel. The eluent in the elution cavity (8) is then drawn into the cavity of the shaft body (101) through the suction module (2). Fix the central shaft and rotate the main box (3) clockwise so that one of the liquid distribution channels connects to the central channel. The eluent in the cavity of the shaft (101) is injected into the detection tube (13) through the liquid distribution channel via the suction module (2). The eluent is injected into each detection tube (13) in sequence. Fix the central shaft and rotate the main box (3) counterclockwise to connect the drying cavity (7) with the central flow channel. Use the suction module (2) to inject the excess eluent in the shaft body (101) cavity into the drying cavity (7). Fix the central shaft and rotate the main box (3) clockwise to connect the paraffin oil cavity (16) with the central flow channel, and draw the paraffin oil into the shaft body (101) cavity through the suction module (2); Fix the central shaft and rotate the main box (3) clockwise to connect the liquid distribution channel (9) with the central flow channel, so that the suction module (2) injects the paraffin oil in the cavity of the shaft (101) into the detection tube (13), and injects the paraffin oil into each detection tube (13) in sequence. Fix the central axis and rotate the main box (3) clockwise to connect the pyrolysis cavity (4) to the central flow channel.

Images