An electrochemical detection device and method for vitamin E

Abstract

This invention relates to the field of electrochemical detection technology for vitamin E, specifically to an electrochemical detection device and method for vitamin E. The method includes: utilizing a detection system composed of a glassy carbon electrode, a saturated calomel electrode, and a platinum wire electrode; performing a series of optimizations and screenings to determine the SWV electrochemical detection method; and electrode pretreatment: using 0.05 μm alumina powder to perform circular polishing on the glassy carbon electrode in both directions for 3-4 minutes, followed by ultrasonic cleaning with anhydrous ethanol and pure water for three minutes each time. Finally, the electrode is scanned using the SWV method in a prepared buffer solution until it stabilizes. The beneficial effects are: this invention is convenient and simple to operate, has a simple formulation that greatly optimizes the detectable limit, has low manufacturing cost and is readily available, and requires no electrode modification; the lowest detectable concentration is far below the minimum normal range of vitamin E in human blood.

Description

Technical Field

[0001] This invention relates to the field of electrochemical detection technology for vitamin E, specifically to an electrochemical detection device and method for vitamin E. Background Technology

[0002] Vitamin E is a fat-soluble vitamin, and its hydrolysis product is tocopherol.

[0003] For the detection of vitamins in the human body, there are currently some methods such as microbial detection, ultraviolet spectrophotometry, fluorescence method, gas chromatography, high performance liquid chromatography, and electrochemical method;

[0004] However, the above-mentioned detection methods are complicated to operate and not easy to master. Furthermore, existing mixed solution devices require measuring an appropriate amount of solution and pouring it into a mixing container before mixing the various solutions. As a result, some solution remains on the inner wall of the container, causing the actual amount of solution mixed to differ from the required amount, thus affecting the accuracy of the experiment. Summary of the Invention

[0005] The purpose of this invention is to provide an electrochemical detection device and method for vitamin E, so as to solve the problems mentioned in the background art.

[0006] To achieve the above objectives, the present invention provides the following technical solution: an electrochemical detection device and method for vitamin E, characterized in that it includes: utilizing a detection system composed of a glassy carbon electrode, a saturated calomel electrode, and a platinum wire electrode, and performing a series of optimization screenings to determine the SWV electrochemical detection method; electrode pretreatment: the glassy carbon electrode is polished in a circular pattern using 0.05 μm alumina powder for 3-4 minutes, then ultrasonically cleaned with anhydrous ethanol and pure water for three minutes in sequence, and finally scanned using the SWV method in a prepared buffer solution until the electrode is stable; SWV electrochemical parameters: potential range -1-1V, potential increment 0.004-0.05V, amplitude 0.01-0.1V, frequency 30-250Hz, settling time 0-30s, enrichment voltage 0.01-0.3V, enrichment time 30-200s; buffer solution formulation: one or two of potassium dihydrogen phosphate buffer solution, potassium hydrogen phosphate buffer solution, or citrate buffer solution mixed with anhydrous ethanol. Mix in a ratio of 0.1:9.9-9:1; add 0.05mol / L-0.5mol / L of dihydrogen phosphate and dihydrogen phosphate, which can be potassium dihydrogen phosphate, sodium dihydrogen phosphate, dipotassium hydrogen phosphate, or disodium hydrogen phosphate, respectively. Any mixture of these can form dihydrogen phosphate buffer, dihydrogen phosphate buffer, potassium phosphate buffer, or sodium phosphate buffer. The citrate buffer solution is formulated by mixing citric acid solution and sodium citrate solution in any molar ratio using a mixing device. Anhydrous ethanol can be benzene, toluene, pentane, hexane, chlorobenzene, dimethylformamide, propylene oxide, etc.; acetone, acetonitrile, pyridine, or phenol.

[0007] Preferably, the mixing device includes a support rod, a rotating rod is provided on the surface of the support rod, the rotating rod is inserted into the surface of the support rod and can rotate on the surface of the support rod, a sleeve ring is fixed at the end of the rotating rod, a connecting rod is inserted into the inside of the sleeve ring, a measuring bottle is fixed at the end of the connecting rod, an annular half tube is fixed at the top of the measuring bottle, an inclined plate is provided inside the measuring bottle, a second support rod is fixed inside the measuring bottle, a screw is provided between multiple sets of second support rods, a blocking block is fixed at the bottom of the screw, and a through hole is opened on the surface of the inclined plate.

[0008] Preferably, the surface of the support rod is provided with a first sliding groove, and a lifting rod is inserted into the first sliding groove. The width of the first sliding groove is greater than the width of the lifting rod, and the support rod can move in the first sliding groove. The surface of the lifting rod is provided with a rotating ring, which can rotate on the surface of the lifting rod. There are two sets of rotating rings. A first stabilizing rod is fixed on the surface of one set of rotating rings, and a second stabilizing rod is fixed on the surface of the other set of rotating rings.

[0009] Preferably, hooks are fixed to the ends of the first and second stabilizer rods, and the hooks can be locked onto the surface of the rotating rod. A first extension plate is fixed to the surface of the lifting rod, and a first elastic sheet is provided on the surface of the first extension plate. There are two sets of the first elastic sheets, and the two sets of the first elastic sheets can be locked onto the surface of the measuring bottle.

[0010] Preferably, a second extension plate is fixed to the surface of the lifting rod, and a second elastic sheet is fixed to the surface of the second extension plate. There are two sets of the second elastic sheets, and the two sets of second elastic sheets can be locked onto the surface of the mixing bottle. The mixing bottle is fixed to the bottom of the connecting rod, and the interior of the connecting rod communicates with the interior of the mixing bottle and the interior of the measuring bottle.

[0011] Preferably, the bottom of the second extension plate is provided with a horizontal plate, and the surface of the horizontal plate is provided with a rotating plate. The rotating plate can rotate at the bottom of the horizontal plate, and the end of the rotating plate is connected to a motor, which drives the rotating plate to rotate.

[0012] Preferably, the surface of the rotating plate is fixed with a first tooth, and multiple sets of the first tooth are provided. The first tooth is distributed on half of the surface of the rotating plate and rotates with the rotating plate. The surface of the horizontal plate is provided with a second sliding groove.

[0013] Preferably, a movable block is inserted into the second slide groove, the movable block can slide in the second slide groove, and a spring is provided in the second slide groove, one end of the spring is connected to the surface of the movable block, and the other end is connected to the inner wall of the second slide groove.

[0014] Preferably, the surface of the movable block is provided with a second tooth, and multiple sets of the second tooth are provided, which mesh with the first tooth on the surface of the rotating plate.

[0015] Preferably, a support ring is fixed to the end of the second support rod, a screw is screwed onto the surface of the support ring, and an annular half-tube is provided at the end of the measuring bottle.

[0016] Compared with the prior art, the beneficial effects of the present invention are: the present invention is convenient and simple to operate, the formula is simple and can greatly optimize the detectable limit, the manufacturing cost is low and readily available, the electrode does not require any modification, and the lowest detectable concentration is far lower than the minimum value of the normal range of vitamin E in human blood. When mixing solutions, different solutions are poured into the measuring bottle, the screw is turned, the blockage block no longer blocks the through hole, allowing the solution in the measuring bottle to enter the mixing bottle. The lifting rod is moved so that the hook is hooked on the surface of the rotating rod, the second elastic plate is locked on the surface of the mixing bottle, and the rotating plate rotates. Then, the first tooth engages with the second tooth to drive the moving block to move. The spring is compressed, and the spring pushes the moving block, causing the moving block to move in the second slide groove. This causes the rotating rod to vibrate, resulting in the vibration of the mixing bottle, which fully mixes the solution in the mixing bottle. Tightening the screw causes the blocking block to block the through hole, and the solution on the surface of the inclined plate flows into the annular half-tube. Rotating the rotating rod causes the solution in the mixing bottle to enter the measuring bottle, completely mixing the solution on the inner wall of the measuring bottle. The first elastic plate is stuck on the surface of the measuring bottle, causing the measuring bottle to rotate and completely mixing the solution to be mixed. Attached Figure Description

[0017] Figure 1 This is a schematic diagram showing the corresponding serial number of VE measured at 50 μmol / L with different electrochemical parameters on the horizontal axis and the average peak current value corresponding to the horizontal axis on the vertical axis.

[0018] Figure 2 This is a schematic diagram of the present invention, where the horizontal axis represents the corresponding serial number of VE measured at 20 μmol / L with different electrochemical parameters, and the vertical axis represents the average peak current value corresponding to the horizontal axis.

[0019] Figure 3 This is a schematic diagram showing the corresponding serial numbers of VE at 50 μmol / L measured with different concentration buffer formulations in this invention, and the average peak current value corresponding to the horizontal axis.

[0020] Figure 4 This is a schematic diagram showing the corresponding serial numbers of VE at 20 μmol / L measured with different concentration buffer formulations in this invention, and the average peak current value corresponding to the horizontal axis.

[0021] Figure 5 This is a three-dimensional structural diagram of the mixing device of the present invention;

[0022] Figure 6 This is a schematic diagram of the three-dimensional structure of the measuring bottle of the present invention;

[0023] Figure 7 This is a schematic diagram of the cross-sectional structure of the measuring bottle of the present invention;

[0024] Figure 8 For the present invention Figure 5Enlarged schematic diagram of the structure at point A in the middle;

[0025] Figure 9 This is a bottom-view three-dimensional structural diagram of the mixing device of the present invention;

[0026] Figure 10 For the present invention Figure 9 Enlarged schematic diagram of the structure at point B.

[0027] In the diagram: 1. Support rod; 2. Connecting rod; 3. Measuring bottle; 4. Sleeve ring; 5. Rotating rod; 6. Screw; 7. Second support rod; 8. Support ring; 9. Annular half-tube; 10. Inclined plate; 11. Through hole; 12. Blocking block; 13. First stabilizing rod; 14. Hook; 15. Second stabilizing rod; 16. First slide groove; 17. Lifting rod; 18. Rotating ring; 19. First elastic plate; 20. First extension plate; 21. Second extension plate; 22. Second elastic plate; 23. Rotating plate; 24. First tooth; 25. Second tooth; 26. Moving block; 27. Spring; 28. Second slide groove. Detailed Implementation

[0028] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the technical solutions of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0029] Example 1

[0030] The mixing device includes a support rod 1, a rotating rod 5 on the surface of the support rod 1, the rotating rod 5 being inserted into the surface of the support rod 1 and capable of rotating on the surface of the support rod 1, a sleeve ring 4 fixed to the end of the rotating rod 5, a connecting rod 2 inserted inside the sleeve ring 4, a measuring bottle 3 fixed to the end of the connecting rod 2, an annular half-tube 9 fixed to the top of the measuring bottle 3, an inclined plate 10 inside the measuring bottle 3, a second support rod 7 fixed inside the measuring bottle 3, a screw 6 between multiple sets of second support rods 7, a blocking block 12 fixed to the bottom of the screw 6, a through hole 11 on the surface of the inclined plate 10, a first groove 16 on the surface of the support rod 1, a lifting rod 17 inserted into the first groove 16, the width of the first groove 16 being greater than the width of the lifting rod 17, and the support rod 1 being able to move within the first groove 16, a rotating ring 18 on the surface of the lifting rod 17, the rotating ring 18 being able to move within the first groove 16, and a rotating ring 18 on the surface of the lifting rod 17. The surface of the lowering rod 17 rotates. The rotating ring 18 has two sets. One set of rotating ring 18 has a first stabilizing rod 13 fixed to its surface, and the other set of rotating ring 18 has a second stabilizing rod 15 fixed to its surface. The ends of the first stabilizing rod 13 and the second stabilizing rod 15 are fixed with hooks 14, which can be locked onto the surface of the rotating rod 5. The surface of the lifting rod 17 has a first extension plate 20 fixed to its surface. The surface of the first extension plate 20 is provided with a first elastic sheet 19. There are two sets of the first elastic sheet 19, which can be locked onto the surface of the measuring bottle 3. The surface of the lifting rod 17 has a second extension plate 21 fixed to its surface. The surface of the second extension plate 21 has a second elastic sheet 22 fixed to its surface. There are two sets of the second elastic sheet 22, which can be locked onto the surface of the mixing bottle. The mixing bottle is fixed to the bottom of the connecting rod 2, and the interior of the connecting rod 2 communicates with the interior of the mixing bottle and the interior of the measuring bottle 3.

[0031] Example 2

[0032] The bottom of the second extension plate 21 is provided with a horizontal plate, and a rotating plate 23 is provided on the surface of the horizontal plate. The rotating plate 23 can rotate at the bottom of the horizontal plate. A motor is connected to the end of the rotating plate 23, and the motor drives the rotating plate 23 to rotate. The surface of the rotating plate 23 is fixed with first teeth 24. Multiple sets of first teeth 24 are provided and distributed on half of the surface of the rotating plate 23. The first teeth 24 rotate with the rotating plate 23. The surface of the horizontal plate is provided with a second sliding groove 28, and a moving block 2 is inserted into the second sliding groove 28. 6. The movable block 26 can slide in the second slide groove 28. A spring 27 is provided in the second slide groove 28. One end of the spring 27 is connected to the surface of the movable block 26, and the other end is connected to the inner wall of the second slide groove 28. The surface of the movable block 26 is provided with second teeth 25. Multiple sets of second teeth 25 are provided. The second teeth 25 mesh with the first teeth 24 on the surface of the rotating plate 23. The end of the second support rod 7 is fixed with a support ring 8. The screw 6 is screwed onto the surface of the support ring 8. The end of the measuring bottle 3 is provided with an annular half tube 9.

[0033] Different DPV electrochemical parameters:

[0034] Electrochemical parameters: potential range -1 to 1V, settling time 2s, enrichment voltage 0.05V, enrichment time 150s;

[0035] Buffer formulation: Potassium dihydrogen phosphate buffer solution and dipotassium hydrogen phosphate buffer solution are mixed with anhydrous ethanol at a ratio of 1:1; the amount added is 0.1 mol / L.

[0036] Examples of different electrochemical parameters

[0037]

[0038] The results of testing 50umol / L of vitamin C are as follows:

[0039]

[0040] The results of testing 20 μmol / L of vitamin C are as follows:

[0041]

[0042] Different buffer formulations:

[0043] Electrochemical parameters: potential range -1 to 1V, potential increment 0.004V, amplitude 0.5V, frequency 150Hz, settling time 2s, enrichment voltage 0.05V, enrichment time 150s.

[0044] The results of testing 50 μmol / L vitamin E with different buffer formulations are as follows:

[0045]

[0046] The results of testing 20 μmol / L vitamin E with different buffer formulations are as follows:

[0047]

[0048] (1) The following conditions shall be adopted

[0049] 1) Electrochemical parameters: potential range -1 to 1V, potential increment 0.004V, amplitude 0.5V, frequency 150Hz, rest time 2s, enrichment voltage 0.05V, enrichment time 150s.

[0050] 2) Buffer formulation: Potassium dihydrogen phosphate buffer solution and dipotassium hydrogen phosphate buffer solution are mixed with anhydrous ethanol at a ratio of 1:1; the amount added is 0.1 mol / L.

[0051] 3) Different concentrations of vitamin E were tested as follows:

[0052]

[0053] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. An electrochemical detection device for vitamin E, characterized in that, include: A support rod (1) is provided with a rotating rod (5) on its surface. The rotating rod (5) is inserted into the surface of the support rod (1) and can rotate on the surface of the support rod (1). A sleeve ring (4) is fixed at the end of the rotating rod (5). A connecting rod (2) is inserted into the inside of the sleeve ring (4). A measuring bottle (3) is fixed at the end of the connecting rod (2). An annular half tube (9) is fixed at the top of the measuring bottle (3). An inclined plate (10) is provided inside the measuring bottle (3). A second support rod (7) is fixed inside the measuring bottle (3). A screw (6) is provided between multiple sets of second support rods (7). A blocking block (12) is fixed at the bottom of the screw (6). A through hole (11) is opened on the surface of the inclined plate (10). A first groove (16) is provided on the surface of the support rod (1), and a lifting rod (17) is inserted into the first groove (16). The width of the first groove (16) is greater than the width of the lifting rod (17), and the support rod (1) can move in the first groove (16). A rotating ring (18) is provided on the surface of the lifting rod (17). The rotating ring (18) can rotate on the surface of the lifting rod (17). There are two sets of rotating rings (18). A first stabilizing rod (13) is fixed on the surface of one set of rotating rings (18), and a second stabilizing rod (15) is fixed on the surface of the other set of rotating rings (18). Hooks (14) are fixed at the ends of the first stabilizing rod (13) and the hooks (14) can be locked on the surface of the rotating rod (5) for lifting. A first extension plate (20) is fixed to the surface of the rod (17). A first elastic sheet (19) is provided on the surface of the first extension plate (20). There are two sets of the first elastic sheets (19). The two sets of the first elastic sheets (19) can be locked onto the surface of the measuring bottle (3). A second extension plate (21) is fixed to the surface of the lifting rod (17). A second elastic sheet (22) is fixed to the surface of the second extension plate (21). There are two sets of the second elastic sheets (22). The two sets of the second elastic sheets (22) can be locked onto the surface of the mixing bottle. The mixing bottle is fixed to the bottom of the connecting rod (2). The inside of the connecting rod (2) is connected to the inside of the mixing bottle. The inside of the connecting rod (2) is connected to the inside of the measuring bottle (3). A horizontal plate is provided at the bottom of the second extension plate (21). A rotating plate (23) is provided on the surface of the horizontal plate. The rotating plate (23) can rotate at the bottom of the horizontal plate. The end of the rotating plate (23) is connected to a motor, which drives the rotating plate (23) to rotate. The surface of the rotating plate (23) is fixed with a first tooth (24). There are multiple sets of first teeth (24). The first teeth (24) are distributed on half of the surface of the rotating plate (23). The first teeth (24) rotate with the rotating plate (23). A second sliding groove (28) is provided on the surface of the horizontal plate. A moving block (26) is inserted into the second sliding groove (28). The moving block (26) can slide in the second sliding groove (28). A spring (27) is provided in the second sliding groove (28). One end of the spring (27) is connected to the surface of the moving block (26).The other end is connected to the inner wall of the second chute (28). The surface of the moving block (26) is provided with second teeth (25). Multiple sets of second teeth (25) are provided. The second teeth (25) mesh with the first teeth (24) on the surface of the rotating plate (23). The end of the second support rod (7) is fixed with a support ring (8). The screw (6) is screwed onto the surface of the support ring (8). The end of the measuring bottle (3) is provided with an annular half-tube (9).

2. A detection method for vitamin E using the electrochemical detection device described in claim 1, characterized in that: This study utilizes a detection system comprised of glassy carbon electrodes, saturated calomel electrodes, and platinum wire electrodes. A series of optimizations and screenings were conducted to develop an SWV electrochemical detection method. Electrode pretreatment involved grinding the glassy carbon electrode in a circular motion with 0.05 μm alumina powder for 3-4 minutes, followed by ultrasonic cleaning with anhydrous ethanol and pure water for three minutes each. The electrode was then scanned using the SWV method in a prepared buffer solution until it stabilized. SWV electrochemical parameters were as follows: potential range -1 to 1 V, potential increment 0.004 to 0.05 V, amplitude 0.01 to 0.1 V, frequency 30 to 250 Hz, settling time 0 to 30 s, enrichment voltage 0.01 to 0.3 V, and enrichment time 30 to 200 s. The buffer solution consisted of one or two of the following: potassium dihydrogen phosphate buffer, dipotassium hydrogen phosphate buffer, or citrate buffer, mixed with anhydrous ethanol. Alcohols are mixed in a ratio of 0.1:9.9-9:1; the amount added is 0.05mol / L-0.5mol / L. Dihydrogen phosphate and dihydrogen phosphate are potassium dihydrogen phosphate, sodium dihydrogen phosphate, dipotassium hydrogen phosphate, and disodium hydrogen phosphate, respectively. They are mixed in any way to form dihydrogen phosphate buffer, dihydrogen phosphate buffer, potassium phosphate buffer, and sodium phosphate buffer. The citrate buffer solution is formulated by mixing citric acid solution and sodium citrate solution in any molar ratio using a mixing device. The organic reagents include anhydrous ethanol (benzene, toluene, pentane, hexane, chlorobenzene, dimethylformamide, propylene oxide), acetone, acetonitrile, pyridine, and phenol.

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