A method for identifying gene duplication and functional differentiation in Rosaceae genera.
By analyzing MAPK family proteins in roses and Arabidopsis thaliana, constructing phylogenetic trees, and combining them with PCR detection, the problem of identifying gene replication and functional differentiation in Rosaceae plants was solved. It was found that the ANP3 gene replicates and functionally differentiates in Rosaceae, providing a basis for quality optimization.
Patent Information
- Application Number
- CN202310510670.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2023-05-08
- Publication Date
- 2026-06-30
- Estimated Expiration
- 2043-05-08
AI Technical Summary
The lack of effective methods to identify gene duplication and functional differentiation in Rosaceae plants has hampered molecular approaches for quality optimization of these plants.
By analyzing MAPK family proteins in rose and Arabidopsis thaliana, we identified HT1, ANP3, Raf42, and Raf24 proteins that are doubled and functionally differentiated in rose. We then extended these proteins to other Rosaceae species using bioinformatics and molecular biology techniques, constructed a ML phylogenetic tree, and visualized it. We also analyzed cis-acting elements on promoters by detecting gene expression changes using real-time quantitative PCR.
This study enabled the rapid identification of replicated and functionally differentiated genes in Rosaceae plants, revealing that the ANP3 gene replicates and exhibits functional differentiation throughout the Rosaceae family, providing a foundation for further research on quality optimization of Rosaceae plants.