A magnetic separation, cleaning and mixing system for chemiluminescence immunoassay

Abstract

The application discloses a magnetic separation, cleaning and mixing system which can be used for chemiluminescence immunoassay and relates to the technical field of automatic biochemical analysis. The magnetic separation, cleaning and mixing system comprises a lower bottom plate, an upper bottom plate arranged above the lower bottom plate, a rotating shaft rotatably installed with the upper bottom plate, a disc body rotatably installed with the rotating shaft and installed on the upper bottom plate, and a magnetic separation assembly comprising three magnets, wherein the three magnets are fixedly installed on the disc body and arranged along the radial direction of the disc body, the gap between adjacent two magnets is a first channel and a second channel respectively, the radius of the first channel is larger than that of the second channel, and a rotating disc is installed on the rotating shaft and is provided with a plurality of first placing holes and second placing holes.

Description

Technical Field

[0001] This invention relates to the field of automated biochemical analysis technology, and more specifically, to a magnetic separation, cleaning, and mixing system that can be used for chemiluminescence immunoassay. Background Technology

[0002] Chemiluminescent immunoassay, also known as chemiluminescent immunoassay, is an immunoassay method that directly labels antigens or antibodies with chemiluminescent agents. A chemiluminescent immunoassay analyzer consists of two parts: an immunoreaction system and a chemiluminescent analysis system. The chemiluminescent analysis system utilizes the catalysis of a catalyst and the oxidation of an oxidant to form an excited-state intermediate. When this excited-state intermediate returns to its stable ground state, it emits photons, and the photon yield is measured using a luminescence signal measuring instrument. The immunoreaction system directly labels the luminescent substance (generated as an excited-state intermediate under the excitation of a reactant) onto the antigen (chemiluminescent immunoassay) or antibody (immunochemiluminescent immunoassay), or an enzyme acts on the luminescent substrate.

[0003] Magnetic separation cleaning is a commonly used structure in chemiluminescence immunoassay, which aims to separate magnetic microparticle complexes carrying the analyte from other reaction solutions. However, most magnetic separation cleaning in existing chemiluminescence immunoassay analyzers uses single-turn, single-sided adsorption, which has poor adsorption effect and low efficiency. Summary of the Invention

[0004] The purpose of this invention is to provide a magnetic separation, cleaning, and mixing system that can be used for chemiluminescence immunoassay, thereby solving the technical problems of poor adsorption effect and low efficiency of the magnetic separation and cleaning mechanism in existing chemiluminescence immunoassay analyzers.

[0005] This invention is achieved through the following technical solution:

[0006] A magnetic separation, washing, and mixing system for use in chemiluminescent immunoassay includes:

[0007] Bottom plate;

[0008] The upper base plate is positioned above the lower base plate;

[0009] A rotating shaft passes through the upper base plate and is rotatably mounted to the upper base plate;

[0010] The disc body is fixedly mounted on the upper base plate and rotatably mounted with the rotating shaft;

[0011] A magnetic separation assembly includes three magnets, all of which are arc-shaped. The three magnets are fixedly mounted on the disk body and arranged along the radial direction of the disk body. The three magnets are spaced apart, and the gap between two adjacent magnets is a first channel and a second channel, respectively. The radius of the first channel is larger than the radius of the second channel.

[0012] A turntable is fixedly mounted on the rotating shaft and located above the disc body. The turntable has multiple first placement holes and second placement holes. The annulus containing the axes of the multiple first placement holes is located within the annulus of the first channel, and the annulus containing the axes of the multiple second placement holes is located within the annulus of the second channel.

[0013] A heating element is installed on the outer wall of the plate body and is electrically connected to a power source.

[0014] Furthermore, the number of magnetic separation components is four sets, the four sets of magnetic separation components are arranged at intervals, and the four sets of magnetic separation components are arranged in an arc shape with the central axis of the disk as the center.

[0015] Furthermore, it also includes:

[0016] The lifting mechanism is fixedly installed on the upper base plate.

[0017] A frame is installed at the output end of the lifting mechanism, which is used to drive the frame to slide up and down.

[0018] A waste liquid needle assembly is mounted on the frame. The waste liquid needle assembly includes two waste liquid needles, the axes of which are located on the annulus containing the first channel and the annulus containing the second channel, respectively.

[0019] Furthermore, there are four sets of waste liquid needles, all of which are installed on the frame and located on one side of the four sets of magnetic separation components.

[0020] Furthermore, it also includes:

[0021] The second mounting plate is fixedly mounted on the disk body. The second mounting plate has a plurality of first arc-shaped holes and second arc-shaped holes. The radius of the first arc-shaped hole is the same as the radius of the first channel, and the radius of the second arc-shaped hole is the same as the radius of the second channel.

[0022] A guide sleeve is fixedly installed on the second mounting plate. Each waste liquid needle is provided with a corresponding guide sleeve. A through hole is opened at the center of the top of each guide sleeve. The axis of each through hole is on the same straight line as the axis of the corresponding waste liquid needle.

[0023] Furthermore, it also includes:

[0024] An adjusting screw is installed at one end of the frame near the lifting mechanism, and the adjusting screw is used to adjust the levelness of the frame.

[0025] Furthermore, it also includes a liquid addition assembly, which comprises:

[0026] The base is mounted on the second mounting plate and is located on one side of the guide sleeve;

[0027] The first liquid filling tube has a first circular hole on its base, the axis of which is located on the annulus where the first channel is located, and the first liquid filling tube is installed in the first circular hole.

[0028] The second liquid filling tube has a second circular hole on its base, the axis of which is located on the annulus where the second channel is located, and the second liquid filling tube is installed inside the second circular hole.

[0029] Furthermore, it also includes a dual mixing component, the dual mixing component comprising:

[0030] A first mounting plate is mounted on the lower base plate;

[0031] The mixing motors are two in number, and both mixing motors are slidably mounted on the first mounting plate.

[0032] The cup holders are of two types, and the two cup holders are respectively mounted on the rotating shafts of the two mixing motors. The two cup holders are a first cup holder and a second cup holder. The axis of the first cup holder is on the same straight line as the axis of the first circular hole, and the axis of the second cup holder is on the same straight line as the axis of the second circular hole.

[0033] A lead screw motor is mounted on the first mounting plate and located below the mixing motor. The lead screw motor is used to drive the mixing motor to move up and down.

[0034] Furthermore, the dual mixing assembly also includes:

[0035] A guide rail is installed on the upper part of the first mounting plate;

[0036] A connecting plate is slidably mounted on the guide rail, the output shaft of the lead screw motor is mounted on the lower part of the connecting plate, and the two mixing motors are mounted on the upper part of the connecting plate;

[0037] The first screw, there are two first screws, and the two first screws are respectively installed on the same side of the two cup holders;

[0038] Two springs are provided, with one end of each spring respectively mounted on one of the two first screws.

[0039] The other ends of the two springs are respectively mounted on the outer walls of the two cup holders.

[0040] Furthermore, the number of liquid dispensing components is four sets, and the four sets of liquid dispensing components are respectively located on one side of the four sets of waste liquid needles. The four sets of liquid dispensing components are the first liquid dispensing component, the second liquid dispensing component, the third liquid dispensing component, and the fourth liquid dispensing component.

[0041] The number of dual mixing components is three, and the three dual mixing components are arranged sequentially below the second liquid addition component, the third liquid addition component and the fourth liquid addition component;

[0042] The axis of each first cup holder is on the same straight line as the axis of the corresponding first circular hole, and the axis of each second cup holder is on the same straight line as the axis of the corresponding second circular hole.

[0043] Compared with the prior art, the present invention has the following beneficial effects:

[0044] The magnetic separation cleaning and mixing system for chemiluminescence immunoassay provided by this invention includes a lower base plate, an upper base plate, a rotating shaft, a disk, a magnetic separation assembly, a turntable, and a heating element. The upper base plate is positioned above the lower base plate. The rotating shaft passes through the upper base plate and is rotatably mounted thereto. The disk is fixedly mounted on the upper base plate and rotatably mounted thereto. The magnetic separation assembly includes three magnets, all of which are arc-shaped and fixedly mounted on the disk, arranged radially along the disk. The three magnets are spaced apart, with the gap between two adjacent magnets being a first channel and a second channel, respectively. The radius of the first channel is larger than the radius of the second channel. The turntable is fixedly mounted on the rotating shaft and positioned above the disk. The turntable has multiple first placement holes and second placement holes. The radius of the circle containing the axis of the multiple first placement holes is equal to the radius of the first channel, and the radius of the circle containing the axis of the multiple second placement holes is equal to the radius of the second channel. The heating element is mounted on the outer wall of the disk and is electrically connected to a power source.

[0045] With the above structure, when magnetic separation is required, the circuit between the heating element and the power supply is first connected to raise the temperature inside the disk to 37 degrees Celsius, allowing for better reaction. Then, the sample to be separated is placed into the first and second placement holes on the turntable through the reaction cup. The rotating shaft is then driven to rotate, causing the turntable mounted on the shaft to rotate as well. The turntable carries the reaction cup to the first and second channels, where magnetic particles are adsorbed by magnets. After adsorption, the magnetic particles are located on the side walls of the reaction cup under the influence of the magnetic field. The turntable continues to rotate, carrying the reaction cup to the next position for further processing. Because both sides of the first and second channels are magnets, double-sided adsorption is achieved, reducing the magnetic bead loss rate and enhancing the adsorption effect. Furthermore, the multiple placement holes and multiple channels allow for simultaneous adsorption of multiple reaction cups, improving adsorption efficiency. Therefore, by using three magnets, the magnetic separation, cleaning, and mixing system for chemiluminescence immunoassay provided by this invention achieves double-loop, double-sided adsorption, enhancing the adsorption effect and improving adsorption efficiency. Attached Figure Description

[0046] The accompanying drawings, which are included to provide a further understanding of embodiments of the invention and form part of this application, do not constitute a limitation thereof. In the drawings:

[0047] Figure 1 This is a schematic diagram of the structure of a magnetic separation, cleaning, and mixing system for chemiluminescent immunoassay provided in an embodiment of the present invention;

[0048] Figure 2 This is another structural schematic diagram of a magnetic separation, cleaning, and mixing system for chemiluminescent immunoassay provided in an embodiment of the present invention;

[0049] Figure 3 A cross-sectional view of a magnetic separation, cleaning, and mixing system for chemiluminescent immunoassay provided in an embodiment of the present invention;

[0050] Figure 4 This is a schematic diagram of the structure of the magnetic adsorption component provided in an embodiment of the present invention;

[0051] Figure 5 A schematic diagram of the installation structure of the frame, lifting mechanism, waste liquid needle and guide sleeve provided in an embodiment of the present invention;

[0052] Figure 6 This is a schematic diagram of the liquid addition assembly provided in an embodiment of the present invention;

[0053] Figure 7 This is a schematic diagram of the structure of the dual mixing component provided in an embodiment of the present invention.

[0054] The attached diagram shows the markings and corresponding component names:

[0055] 1-Lower base plate, 2-Upper base plate, 3-Rotating shaft, 4-Disc body, 5-Magnet, 51-First channel, 52-Second channel, 6-Turntable, 61-First placement hole, 62-Second placement hole, 63-Heating element, 7-Frame, 8-Lifting mechanism, 9-Waste liquid needle, 10-Second mounting plate, 101-First arc-shaped hole, 102-Second arc-shaped hole, 20-Guide sleeve, 30-Adjusting screw, 40-First liquid addition assembly, 401-Base, 402-First liquid addition tube, 403-Second liquid addition tube, 50-Dual mixing assembly, 501-First mounting plate, 502-Mixing motor, 503-Cup holder, 504-Screw motor, 505-Guide rail, 506-Connecting plate, 507-First screw, 508-Spring, 60-Second liquid addition assembly, 70-Third liquid addition assembly, 80-Fourth liquid addition assembly. Detailed Implementation

[0056] To make the objectives, technical solutions, and advantages of the present invention clearer, the present invention will be further described in detail below with reference to the embodiments and accompanying drawings. The illustrative embodiments and descriptions of the present invention are only used to explain the present invention and are not intended to limit the present invention.

[0057] It should be noted that when a component is referred to as being "fixed to" or "set on" another component, it can be directly or indirectly attached to that other component. When a component is referred to as being "connected to" another component, it can be directly or indirectly connected to that other component.

[0058] It should be understood that the terms "length", "width", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on the present invention.

[0059] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of this invention, "a plurality of" means two or more, unless otherwise explicitly specified.

[0060] Example

[0061] This invention provides a magnetic separation, cleaning, and mixing system for chemiluminescence immunoassay, solving the technical problems of poor adsorption and low efficiency in the magnetic separation and cleaning mechanisms of existing chemiluminescence immunoassay analyzers. The magnetic separation, cleaning, and mixing system for chemiluminescence immunoassay includes a lower base plate 1, an upper base plate 2, a rotating shaft 3, a disk 4, a magnetic separation assembly, a rotating disk 6, and a heating element 63, wherein:

[0062] The bottom plate 1 is a circular structural component.

[0063] The upper base plate 2 is positioned above the lower base plate 1. Specifically, four support columns are fixedly installed between the upper base plate 2 and the lower base plate 1, and the four support columns are located at the four vertices of the same rectangle.

[0064] The rotating shaft 3 passes through the upper base plate 2 and is rotatably mounted to the upper base plate 2. Specifically, the rotating shaft 3 is a stepped shaft. A first mounting hole is provided at the center of the top wall of the upper base plate 2. One end of the rotating shaft 3 extends out of the upper base plate 2 through the first mounting hole, and the shoulder of the rotating shaft 3 abuts against the top wall of the upper base plate 2. A pulley is connected to the end of the rotating shaft 3 near the lower base plate 1. A first drive motor is mounted on the lower base plate 1. The first drive motor is used to drive the pulley.

[0065] The disc body 4 is fixedly mounted on the upper base plate 2 and rotatably mounted with the rotating shaft 3. Specifically, the center of the inner bottom wall of the disc body 4 is...

[0066] A second mounting hole is provided, through which the disc body 4 is rotatably mounted on the rotating shaft 3. More preferably, a bearing is installed between the disc body 4 and the rotating shaft 3, so that when the rotating shaft 3 rotates, the friction between the rotating shaft 3 and the disc body 4 is reduced, which is more conducive to the rotation of the rotating shaft 3. The magnetic separation assembly includes three magnets 5, all of which are arc-shaped and fixedly mounted on a disk 4, arranged radially along the disk 4. The radii of the three magnets 5 increase sequentially in the radial direction away from the rotating shaft 3. The three magnets 5 are spaced apart, with the gaps between adjacent magnets 5 forming a first channel 51 and a second channel 52, respectively. The radius of the first channel 51 is larger than the radius of the second channel 52. Thus, the first channel 51 and the second channel 52 formed by the three spaced magnets 5 enable simultaneous magnetic separation of multiple reaction cups, improving efficiency and making the structure of the magnetic separation, cleaning, and mixing system for chemiluminescence immunoassay provided by this invention more compact and smaller in size. Furthermore, during magnetic separation, both sides of the reaction cup are adorned with magnets 5, achieving bilateral adsorption of the reaction cup, enhancing the adsorption effect, and reducing the magnetic bead loss rate.

[0067] The turntable 6 is fixedly mounted on the rotating shaft 3 and located above the disc body 4. The turntable 6 has multiple first placement holes 61 and second placement holes 62. The annulus containing the axes of the multiple first placement holes 61 is located within the annulus of the first channel 51, and the annulus containing the axes of the multiple second placement holes 62 is located within the annulus of the second channel 52. Optionally, the multiple first placement holes 61 and the multiple second placement holes 62 are arranged in an arc shape. The rotating shaft 3 drives the turntable 6 to rotate, thereby causing the multiple reaction cups placed in the multiple first placement holes 61 and the multiple second placement holes 62 to rotate together, which facilitates the reaction.

[0068] The heating element 63 is installed on the outer wall of the disk body 4 and is electrically connected to the power supply. Optionally, the heating element 63 is a heating film. In this way, by setting the heating film, the temperature inside the disk body 4 is raised to 37 degrees Celsius before magnetic separation is performed. The appropriate temperature further improves the magnetic separation effect.

[0069] With the above structure, when magnetic separation is required, the circuit between the heating element 63 and the power supply is first connected, causing the temperature inside the disk 4 to rise to thirty-seven degrees Celsius, allowing for better reaction. Then, the sample to be separated is placed through the reaction cup into the first placement hole 61 and the second placement hole 62 on the turntable 6. The rotating shaft 3 is then driven to rotate, causing the turntable 6 mounted on the shaft 3 to rotate as well. The turntable 6 carries the reaction cup into the first channel 51 and the second channel 52, where the magnet 5 adsorbs the magnetic particles. After adsorption, the magnetic particles are located under the influence of the magnetic field. On the side walls of both sides of the reaction cup, the turntable 6 continues to rotate, bringing the reaction cup to the next position for further processing. Because both sides of the first channel 51 and the second channel 52 are magnets 5, the purpose of double-sided adsorption is achieved, reducing the magnetic bead loss rate and enhancing the adsorption effect. Furthermore, due to the setting of multiple placement holes and multiple channels, multiple reaction cups can be adsorbed simultaneously, improving the adsorption efficiency. Therefore, through the setting of three magnets 5, the magnetic separation cleaning and mixing system for chemiluminescence immunoassay provided by this invention achieves double-circle double-sided adsorption, enhancing the adsorption effect and improving the adsorption efficiency.

[0070] An optional implementation of this embodiment is as follows: the number of magnetic separation components is four sets, the four sets of magnetic separation components are arranged at intervals, and the four sets of magnetic separation components are arranged in an arc shape with the central axis of the disk body 4 as the center. In this way, by setting multiple sets of magnetic separation components, the sample is magnetically separated multiple times, thereby enhancing the magnetic separation effect.

[0071] An optional implementation of this embodiment is as follows, further comprising a frame 7, a lifting mechanism 8, and a waste liquid needle assembly, wherein:

[0072] The lifting mechanism 8 is fixedly installed on the upper base plate 2.

[0073] The frame 7 is installed at the output end of the lifting mechanism 8, which is used to drive the frame 7 to slide up and down.

[0074] Specifically, a first placement plate is fixedly installed on the upper base plate 2. A second drive motor is installed on the bottom wall of the first placement plate. A first slider is installed on the telescopic end of the second drive motor. Two first slide rails are installed on the side wall of the first placement plate. Two slide grooves adapted to the first slide rails are opened on the side of the first slider near the first slide rails. The first slider is slidably installed on the two first slide rails. The frame 7 includes an outer part, a connecting part, and an installation part. The outer part is a square block, and the installation part is an arc-shaped plate. The arc-shaped plate is fixedly connected to the square block through the connecting part. It should be noted that the arc-shaped plate, the connecting part, and the square block can be connected together by welding, bonding, or hot melting. Of course, they can also be integrally formed. The square block is detachably fixedly installed on the top of the slider. In this way, by driving the second drive motor, the output shaft of the second drive motor is extended and retracted, thereby driving the first slider installed on the second drive motor to move up and down on the slide rails, and thus driving the frame 7 installed on the first slider to move up and down.

[0075] The waste liquid needle assembly is mounted on the frame 7. The assembly includes two waste liquid needles 9, with their axes located on the annulus containing the first channel 51 and the second channel 52, respectively. Optionally, a spring 508 is provided between the waste liquid needle 9 and the frame 7. This spring 508 ensures that the bottom of the waste liquid needle 9 contacts the bottom of the reaction vessel. Furthermore, when the frame 7 descends excessively, the spring 508 acts as a buffer, effectively reducing the likelihood of damage to the waste liquid needle 9.

[0076] Optionally, each waste liquid needle 9 has a liquid inlet groove at its bottom to reduce the chance of remaining reaction liquid at the bottom of the reaction cup.

[0077] Optionally, it also includes an adjusting screw 30, wherein:

[0078] The adjusting screw 30 is installed at one end of the frame 7 near the lifting mechanism 8, that is, the adjusting screw 30 is installed on the square plate. The adjusting screw 30 is used to adjust the level of the frame 7. In this way, when the frame 7 is tilted due to external objects, the adjusting screw 30 can be used to adjust the frame 7 to be completely level, so that all the waste liquid needles 9 installed on the frame 7 are located in the same horizontal plane.

[0079] Optionally, there are four waste liquid needle sets, all of which are installed on the frame 7 and located on one side of the four magnetic separation components. In this way, by setting up multiple waste liquid needle sets corresponding to multiple magnetic separation components, waste liquid can be aspirated again after each magnetic separation of the sample.

[0080] An optional implementation of this embodiment is as follows: it further includes a second mounting plate 10 and a guide sleeve 20, wherein:

[0081] The second mounting plate 10 is fixedly mounted on the disc body 4. The second mounting plate 10 has a plurality of first arc-shaped holes 101 and second arc-shaped holes 102. The radius of the first arc-shaped hole 101 is the same as the radius of the first channel 51, and the radius of the second arc-shaped hole 102 is the same as the radius of the second channel 52. In this way, the arrangement of the first arc-shaped hole 101 and the second arc-shaped hole 102 makes it easy to place the reaction cup into the first placement hole 61 and the second placement hole 62.

[0082] The guide sleeve 20 is fixedly installed on the second mounting plate 10. Each waste liquid needle 9 is provided with a corresponding guide sleeve 20. Each guide sleeve 20 has a through hole at the center of its top end. The axis of each through hole is on the same straight line as the axis of its corresponding waste liquid needle 9. In this way, by setting the guide sleeve 20, when the waste liquid needle 9 penetrates into the bottom of the reaction cup to draw up the waste liquid, it is located in the center of the reaction cup, which prevents the waste liquid needle 9 from approaching the side wall of the reaction cup and sucking up the magnetic beads, thus increasing the magnetic bead loss rate.

[0083] An optional implementation of this embodiment is as follows: it further includes a liquid adding component, which includes a base 401, a first liquid adding pipe 402, and a second liquid adding pipe 403, wherein:

[0084] The base 401 is mounted on the second mounting plate 10 and is located on one side of the guide sleeve 20.

[0085] The base 401 has a first circular hole, the axis of which is located on the annulus where the first channel 51 is located, and the first liquid adding tube 402 is installed in the first circular hole.

[0086] A second circular hole is provided on the base 401, and the axis of the second circular hole is located on the ring where the second channel 52 is located. The second liquid adding tube 403 is installed in the second circular hole.

[0087] With the above structure, after the magnetic separation and waste liquid absorption are completed, the turntable 6 rotates and moves the reaction cup to the bottom of the base 401. The first liquid addition pipe 402 located directly above the first channel 51 and the second liquid addition pipe 403 located directly above the second channel 52 can add cleaning liquid to the reaction cup located in the first channel 51 and the second channel 52 respectively, so as to prevent the cleaning liquid from leaking out.

[0088] An optional implementation of this embodiment is as follows: it further includes a dual mixing component 50, which includes a first mounting plate 501, a mixing motor 502, a cup holder 503, and a lead screw motor 504, wherein:

[0089] The first mounting plate 501 is mounted on the lower base plate 1.

[0090] There are two mixing motors 502, both of which are slidably mounted on the first mounting plate 501. It should be noted that the two mixing motors 502 are electrically connected to the power supply through lines, and each of these two lines is equipped with a switch for turning the power on and off. The switch can be a wireless switch or a wired switch. The two switches are used to control the power on and off of the two mixing motors 502, so as to achieve different speeds for the different mixing motors 502.

[0091] There are two cup holders 503, each mounted on a shaft 3 of a mixing motor 502. These two cup holders are designated as the first and second cup holders, respectively. The axis of the first cup holder is collinear with the axis of the first circular hole, and the axis of the second cup holder is collinear with the axis of the second circular hole. When the turntable 6 moves the reaction cups directly above the two cup holders 503, it drives the two mixing motors 502 upwards until the two reaction cups are placed into the two cup holders 503. After the first and second inlet pipes are inserted into the two reaction cups respectively, the cleaning solution is added. After enough cleaning solution is added, the two mixing motors 502 are driven to move downwards until the first and second inlet pipes leave the two reaction cups. Then, the two mixing motors 502 are driven to start shaking to mix the cleaning solution with the magnetic beads. After thorough mixing, the two mixing motors 502 are driven to move downwards again until the two reaction cups are placed back into the first placement hole 61 and the second placement hole 62, so that the turntable 6 can carry the two reaction cups to the next set of magnetic separation components for the next magnetic separation.

[0092] The lead screw motor 504 is mounted on the first mounting plate 501 and located below the mixing motor 502. The lead screw motor 504 is used to drive the mixing motor 502 to move up and down. By driving the two mixing motors 502 to move up and down through the lead screw motor 504, it is not only convenient to control the displacement distance of the mixing motors 502, but the lead screw motor 504 also has a self-locking function, so that the two mixing motors 502 can be stably stopped at a certain height.

[0093] One embodiment of this invention is as follows: the dual mixing assembly 50 further includes a guide rail 505, a connecting plate 506, a first screw 507, and a spring 508, wherein:

[0094] The guide rail 505 is installed on the upper part of the first mounting plate 501.

[0095] The connecting plate 506 is slidably mounted on the guide rail 505. The output shaft of the lead screw motor 504 is mounted on the lower part of the connecting plate 506, and both mixing motors 502 are mounted on the upper part of the connecting plate 506. In this way, the connecting plate 506 is designed to facilitate the simultaneous driving of the two mixing motors 502 to move together, and to facilitate the control of the two mixing motors 502 to move the same distance, so that the bottom walls of the two cup holders 503 are located on the same plane.

[0096] There are two first screws 507, and the two first screws 507 are respectively installed on the same side of the two cup holders 503.

[0097] There are also two springs 508. One end of each spring 508 is installed on one of the two first screws 507, and the other end of each spring 508 is installed on the outer side wall of each of the two cup holders 503. In this way, the springs 508 are designed so that the cup holders 503 are pulled back by the springs 508 after rotating a certain angle, forming repeated swaying and shaking to enhance the mixing effect.

[0098] One implementation of this embodiment is as follows: There are four sets of liquid addition components. The four sets of liquid addition components are located on one side of the four sets of waste liquid needles. The four sets of liquid addition components are the first liquid addition component 40, the second liquid addition component 60, the third liquid addition component 70 and the fourth liquid addition component 80.

[0099] There are three dual mixing components 50, which are arranged sequentially below the second liquid addition component 60, the third liquid addition component 70 and the fourth liquid addition component 80.

[0100] The axis of each first cup holder is on the same straight line as the axis of the corresponding first circular hole, and the axis of each second cup holder is on the same straight line as the axis of the corresponding second circular hole.

[0101] With the above structure, no cleaning solution is needed during the first magnetic separation, so no mixing is required. The dual mixing component 50 is not installed below the first liquid addition component 40. After the first magnetic separation, the turntable 6 moves the reaction cup to below the first waste liquid needle group to extract the first waste liquid. Then, the turntable 6 moves the reaction cup to below the second liquid addition component 60 to add cleaning solution. Then, the dual mixing component 50 is driven to mix the solution. Then, the next magnetic separation is performed. This cycle is repeated four times to perform four magnetic separations on the sample, thus enhancing the cleaning effect.

[0102] The above specific embodiments further illustrate the purpose, technical solution, and beneficial effects of the present invention. It should be understood that the above description is only a specific embodiment of the present invention and is not intended to limit the scope of protection of the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. A magnetic separation, cleaning, and mixing system for use in chemiluminescence immunoassay, characterized in that, include: Bottom plate (1); The upper base plate (2) is disposed above the lower base plate (1); A rotating shaft (3) passes through the upper base plate (2) and is rotatably mounted with the upper base plate (2); The disc body (4) is fixedly installed on the upper base plate (2) and rotatably mounted with the rotating shaft (3); A magnetic separation assembly includes three magnets (5), all three magnets (5) are arc-shaped, all three magnets (5) are fixedly mounted on the disk body (4) and arranged along the radial direction of the disk body (4), the three magnets (5) are spaced apart, and the gap between two adjacent magnets (5) is a first channel (51) and a second channel (52) respectively, the radius of the first channel (51) is larger than the radius of the second channel (52); A turntable (6) is fixedly installed on the rotating shaft (3) and located above the disc body (4). The turntable (6) has a plurality of first placement holes (61) and second placement holes (62). The annulus containing the axes of the plurality of first placement holes (61) is located within the annulus of the first channel (51), and the annulus containing the axes of the plurality of second placement holes (62) is located within the annulus of the second channel (52). A heating element (63) is installed on the outer side wall of the plate body (4) and is electrically connected to a power source.

2. The magnetic separation, cleaning, and mixing system for chemiluminescent immunoassay according to claim 1, characterized in that, The number of magnetic separation components is four sets, and the four sets of magnetic separation components are arranged at intervals, and the four sets of magnetic separation components are arranged in an arc shape with the central axis of the disk body (4) as the center.

3. The magnetic separation, cleaning, and mixing system for chemiluminescent immunoassay according to claim 1, characterized in that, Also includes: The lifting mechanism (8) is fixedly installed on the upper base plate (2); A frame (7) is installed at the output end of a lifting mechanism (8), which is used to drive the frame (7) to slide up and down. Waste liquid needle assembly, which is installed on the frame (7), includes two waste liquid needles (9), the axes of the two waste liquid needles (9) are respectively located on the ring where the first channel (51) is located and on the ring where the second channel (52) is located.

4. The magnetic separation, cleaning, and mixing system for chemiluminescence immunoassay according to claim 3, characterized in that, The number of waste liquid needle groups is four, and all four waste liquid needle groups are installed on the frame (7) and are located on one side of the four magnetic separation components respectively.

5. The magnetic separation, cleaning, and mixing system for chemiluminescent immunoassay according to claim 4, characterized in that, Also includes: The second mounting plate (10) is fixedly mounted on the disk body (4). The second mounting plate (10) has a plurality of first arc-shaped holes (101) and second arc-shaped holes (102). The radius of the first arc-shaped hole (101) is the same as the radius of the first channel (51), and the radius of the second arc-shaped hole (102) is the same as the radius of the second channel (52). Guide sleeve (20) is fixedly installed on the second mounting plate (10). Each waste liquid needle (9) is provided with a corresponding guide sleeve (20). A through hole is opened at the center of the top of each guide sleeve (20). The axis of each through hole is on the same straight line as the axis of the corresponding waste liquid needle (9).

6. The magnetic separation, cleaning, and mixing system for chemiluminescence immunoassay according to claim 5, characterized in that, Also includes: An adjusting screw (30) is installed on one end of the frame (7) near the lifting mechanism (8). The adjusting screw (30) is used to adjust the levelness of the frame (7).

7. The magnetic separation, cleaning, and mixing system for chemiluminescence immunoassay according to claim 5, characterized in that, It also includes a liquid addition assembly, which comprises: The base (401) is mounted on the second mounting plate (10) and located on one side of the guide sleeve (20); The first liquid filling tube (402) has a first circular hole on the base (401), the axis of the first circular hole is located on the annulus where the first channel (51) is located, and the first liquid filling tube (402) is installed in the first circular hole; The second liquid filling tube (403) has a second circular hole on the base (401), the axis of the second circular hole is located on the ring where the second channel (52) is located, and the second liquid filling tube (403) is installed in the second circular hole.

8. The magnetic separation, cleaning, and mixing system for chemiluminescence immunoassay according to claim 7, characterized in that, It also includes a dual mixing assembly (50), the dual mixing assembly (50) comprising: The first mounting plate (501) is mounted on the lower base plate (1); Two mixing motors (502) are provided, and both mixing motors (502) are slidably mounted on the first mounting plate (501). Cup holder (503), there are two cup holders (503), the two cup holders (503) are respectively installed on the rotating shaft (3) of the two mixing motors (502), the two cup holders (503) are respectively the first cup holder and the second cup holder, the axis of the first cup holder and the axis of the first round hole are on the same straight line, and the axis of the second cup holder and the axis of the second round hole are on the same straight line; A lead screw motor (504) is mounted on the first mounting plate (501) and located below the mixing motor (502). The lead screw motor (504) is used to drive the mixing motor (502) to move up and down.

9. The magnetic separation, cleaning, and mixing system for chemiluminescent immunoassay according to claim 8, characterized in that, The dual mixing assembly (50) further includes: The guide rail (505) is installed on the upper part of the first mounting plate (501); A connecting plate (506) is slidably mounted on the guide rail (505), the output shaft of the lead screw motor (504) is mounted on the lower part of the connecting plate (506), and both mixing motors (502) are mounted on the upper part of the connecting plate (506); The first screw (507) is two in number, and the two first screws (507) are respectively installed on the same side of the two cup holders (503); Spring (508), there are two springs (508), one end of the two springs (508) is respectively mounted on the two first screws (507), and the other end of the two springs (508) is respectively mounted on the outer side wall of the two cup holders (503).

10. The magnetic separation, cleaning, and mixing system for chemiluminescent immunoassay according to any one of claims 1-9, characterized in that, There are four sets of liquid dosing components, which are located on one side of the four sets of waste liquid needles. The four sets of liquid dosing components are the first liquid dosing component (40), the second liquid dosing component (60), the third liquid dosing component (70), and the fourth liquid dosing component (80). There are three dual mixing components (50), which are arranged below the second liquid addition component (60), the third liquid addition component (70) and the fourth liquid addition component (80) in sequence; The axis of each first cup holder is on the same straight line as the axis of the corresponding first circular hole, and the axis of each second cup holder is on the same straight line as the axis of the corresponding second circular hole.

Images