Compositions and methods for assay measurement

TEA-based compositions in ECL assays address the challenge of distinguishing bound and unbound labels without washing, enhancing signal ratios and reducing surfactant dependency, thus improving assay performance and versatility.

JP7875821B2Active Publication Date: 2026-06-18MESO SCALE TECH LLC
3 Cites 0 Cited by

Patent Information

Authority / Receiving Office
JP · JP
Patent Type
Patents
Current Assignee / Owner
MESO SCALE TECH LLC
Filing Date
2021-06-30
Publication Date
2026-06-18

AI Technical Summary

⚠Technical Problem

Existing ECL-based assays on solid surfaces require a washing step to distinguish between bound and unbound ECL labels, which can be cumbersome and lead to high background signals, especially in low-affinity interactions and assays involving sensitive analytes.

⚗Method used

Compositions comprising triethanolamine (TEA) and ionic components, with a pH of 7.0 to 8.0 and minimal buffering, effectively distinguish between bound and unbound ECL labels, allowing for 'no-wash' assay formats and maintaining consistent ECL generation across different surfactant conditions.

🎯Benefits of technology

The TEA-based compositions enhance the ratio of bound-to-unbound ECL signals, reduce the need for surfactants like TRITON® X-100, and improve assay performance, particularly in low-affinity interactions and assays with sensitive analytes, while maintaining consistent ECL production.

✦ Generated by Eureka AI based on patent content.

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The present invention relates to novel compositions comprising an electrochemiluminescence (ECL) coreactant. In embodiments, the compositions further comprise an ionic component, a surfactant, or a combination thereof. In embodiments, the ECL coreactant is triethanolamine (TEA), tert-butyldiethanolamine (tBDEA), methyldibutylethanolamine (MDEA), 3-[bis-(2-hydroxyethyl)-amino]-propane-1-sulfonic acid (DEA-PS), or a combination thereof. Methods of using the compositions and kits containing the compositions are also provided herein.
Need to check novelty before this filing date? Find Prior Art