Microfluidic control system and method of use

Abstract

The application discloses a micro-fluid control system and a use method, and belongs to the technical field of micron-level multiphase dispersion system preparation. The micro-fluid control system comprises a micro-fluid control chip, a plurality of micro-fluid capillary channels are arranged on the micro-fluid control chip, and the micro-fluid capillary channels are used for preparing droplets or bubbles; a continuous phase capillary channel is arranged on the micro-fluid control chip, and the continuous phase capillary channel is used for circulating a continuous phase medium; a micro-injection pump is communicated with the micro-fluid capillary channel, and the micro-injection pump is used for injecting a dispersed phase fluid; and a conventional injection pump is communicated with the continuous phase capillary channel, and the conventional injection pump is used for injecting a continuous phase fluid. The micro-fluid control system can flexibly, conveniently and effectively prepare various multiphase dispersion systems and realize control on physical and chemical properties of the dispersion systems, and solves the problem that multiphase dispersion system preparation is limited in the prior art.

Description

Technical Field

[0001] This invention relates to the field of micron-scale multiphase dispersion system preparation technology, and in particular to a microfluidic control system and its usage method. Background Technology

[0002] The statements in this section merely refer to the background art related to this invention and do not necessarily constitute prior art.

[0003] Dispersed systems are widely present in various aspects of industrial production and human life. The stability of two-phase or even multi-phase dispersed systems is crucial for production activities in fields such as food, pharmaceuticals, cosmetics, and petroleum.

[0004] The theoretical study of the stability of dispersed systems mainly focuses on thermodynamics and kinetics. The DLVO theory, based on kinetic interactions, posits that the stability of colloidal systems primarily depends on the strength of van der Waals forces and double-layer repulsion. With the development of modern experimental measurement techniques, particularly the successful application of atomic force microscopy (AFM) in microscopic mechanical measurements, this theory has been increasingly validated by experimental data and continuously refined. Later research has shown that, in addition to van der Waals and double-layer forces, other structuring forces also significantly influence the stability of dispersed systems; these forces are generally referred to as non-DLVO forces. AFM's mechanical measurement techniques can then be used to measure, analyze, and characterize these microscopic forces.

[0005] Currently, due to technological limitations, the measurement of microscopic interactions between dispersed phases in dispersed systems is mainly conducted on oil droplets and bubbles in aqueous solutions, as well as oil droplets / bubbles and solid particles or walls. However, the measurement of interactions between oil droplets and bubbles in aqueous solutions, water droplets and bubbles where oil is a continuous phase, and two droplets or bubbles with different physicochemical properties is relatively scarce.

[0006] Traditional techniques for preparing micron-sized droplets and bubbles through atomization and ultrasonic oscillation can only produce a single dispersed phase and cannot simultaneously produce two different dispersed phases. Furthermore, the size of the dispersed phase is uncontrollable and unreproducible, and the wettability requirements of the substrate are stringent, which greatly limits related research. Summary of the Invention

[0007] To address the shortcomings of existing technologies, this invention provides a microfluidic control system and its usage method, which can more flexibly, conveniently and effectively prepare various multiphase dispersion systems and achieve control over the physicochemical properties of the dispersion systems. This facilitates the measurement, analysis and characterization of microscopic forces in multiphase dispersion systems using atomic force microscopy (AFM) mechanical measurement technology.

[0008] In a first aspect, the present invention provides a microfluidic control system;

[0009] A microfluidic control system comprises a microfluidic control chip, wherein a plurality of microfluidic capillary channels are arranged on the microfluidic control chip, and a continuous phase capillary channel is arranged on the microfluidic control chip; the microfluidic capillary channels are communicated with microsyringe pumps, and the continuous phase capillary channel is communicated with a conventional syringe pump;

[0010] The microsyringe pumps inject a dispersed phase fluid into the microfluidic capillary channels until the microfluidic capillary channels are filled, and the conventional syringe pump injects a continuous phase fluid into the continuous phase capillary channel until the continuous phase fluid overflows from above the microfluidic control chip to form a continuous phase environment; the injection of the dispersed phase fluid by the microsyringe pumps is continued until the dispersed phase fluid overflows through the microfluidic capillary channels to form droplets or bubbles.

[0011] The size of the droplets or bubbles is controlled by the driving rate and driving stroke of the microsyringe pumps.

[0012] Further, the inner diameter of the microfluidic capillary channels is 8-12 μm, and the inner diameter of the continuous phase capillary channel is 480-520 μm.

[0013] Further, the microsyringe pump comprises a pump body and a microsyringe, the output end of the pump body is communicated with the input end of the microsyringe, and the output end of the microsyringe is communicated with the microfluidic capillary channel through a capillary tube.

[0014] Further, the conventional syringe pump comprises a pump body and a syringe, the output end of the pump body is communicated with the input end of the syringe, and the output end of the syringe is communicated with the continuous phase capillary channel through a capillary tube.

[0015] Further, the continuous phase capillary channel comprises a continuous phase inlet capillary channel and a continuous phase outlet capillary channel, the continuous phase inlet capillary channel and the continuous phase outlet capillary channel are of the same structure and are symmetrically arranged on the microfluidic control chip along the longitudinal center line of the microfluidic control chip.

[0016] In a second aspect, the present application provides a use method of the above microfluidic control system.

[0017] The use method of the microfluidic control system comprises the following steps:

[0018] S1, adjusting the driving rate and stroke, using the microsyringe pump to inject a dispersed phase fluid into a microfluidic capillary channel so that the dispersed phase fluid fills the microfluidic capillary channel;

[0019] S2, adjusting the driving rate and stroke, using a microsyringe pump to inject another dispersed phase fluid into another microfluidic capillary channel, so that the dispersed phase fluid fills the microfluidic capillary channel;

[0020] S3, using a conventional syringe pump to inject a continuous phase fluid into a continuous phase capillary channel, so that the continuous phase fluid fills the continuous phase capillary channel and then overflows from above the microfluidic control chip to form a continuous phase environment;

[0021] S4, continue to inject the dispersed phase fluid, so that the dispersed phase fluid overflows through the microfluidic capillary channel to form droplets or bubbles, and the diameter of the droplets or bubbles is 50-200 microns.

[0022] Further, the S3 is specifically:

[0023] Close the continuous phase outlet capillary channel on the microfluidic control chip, adjust the driving rate and injection volume of the conventional syringe pump, and use the conventional syringe pump to inject a continuous phase fluid into a continuous phase inlet capillary channel, so that the continuous phase fluid fills the continuous phase inlet channel and then overflows from above the microfluidic control chip to form a continuous phase environment.

[0024] Preferably, it further comprises S5;

[0025] S5, open the continuous phase outlet channel on the microfluidic control chip, and continue to inject the continuous phase fluid using the conventional syringe pump, so that the continuous phase fluid flows out through the continuous phase outlet channel.

[0026] Further, the continuous phase fluid is a liquid or a gas, and the dispersed phase fluid is a liquid or a gas.

[0027] The continuous phase fluid and the dispersed phase fluid are not mutually soluble.

[0028] Further, the injection volume of the dispersed phase fluid is 0.0001-0.005 microliters.

[0029] Compared with the prior art, the beneficial effects of the present application are:

[0030] 1. The technical scheme provided by the present application can accurately control the size of droplets or bubbles by setting the driving rate and stroke of the microsyringe pump according to the preparation requirements of the micron-sized multi-phase dispersion system, and the size of the droplets or bubbles can be repeated in different experiments.

[0031] 2. The technical scheme provided by the present application has two microfluidic channels, and can prepare droplets and bubbles of different media or the same medium but different physical and chemical properties, and realize the measurement of micro-forces of two-phase or even multi-phase systems.

[0032] 3、The technical scheme provided by the application has a continuous phase inlet capillary channel and a continuous phase outlet capillary channel, can realize dynamic control of the physical and chemical properties of the continuous phase medium, and can also be used to study the dynamic interaction force between the dispersed media in the micro flow field.

[0033] 4、The technical scheme provided by the application can realize the fixing of droplets and bubbles and the capturing of probes without wetting modification treatment on the surface of the micro fluid capillary channel. BRIEF DESCRIPTION OF DRAWINGS

[0034] The drawings constituting a part of the specification of the application are used to provide further understanding of the application, the illustrative embodiments of the application and the description thereof are used to explain the application, and do not constitute improper limitation on the application.

[0035] Fig. 1 The structural schematic diagram of the micro fluid control chip provided for the embodiment of the application is shown in the figure.

[0036] Fig. 2 The structural schematic diagram of the micro fluid control system provided for the embodiment of the application is shown in the figure.

[0037] Among them, 1 is a continuous phase inlet capillary channel; 2 is a continuous phase outlet capillary channel; 3 is a first micro fluid capillary channel; 4 is a second micro fluid capillary channel; 5 is a conventional injection pump; 6 is a micro injection pump; 7 is a micro fluid control chip. DETAILED DESCRIPTION

[0038] It should be pointed out that the following detailed description is exemplary and is intended to provide further description of the application. Unless otherwise specified, all technical and scientific terms used in the application have the same meaning as generally understood by those skilled in the art to which the application belongs.

[0039] The embodiments in the application and the features in the embodiments can be combined with each other without conflict.

[0040] In combination Figs. 1-2 The application provides a micro fluid control system for preparing a micron-level multi-phase dispersed system; the micro fluid control system comprises a micro fluid control chip 7, two micro fluid capillary channels are formed in the micro fluid control chip 7, and the micro fluid capillary channels are used for preparing droplets or bubbles; in order to facilitate the differentiation of the two micro fluid capillary channels in the following, they are respectively named as a first micro fluid capillary channel 3 and a second micro fluid capillary channel 4; the first micro fluid capillary channel 3 and the second micro fluid capillary channel 4 are of the same structure, are of an L-shaped structure, and are horizontally symmetrically arranged along the transverse center line of the micro fluid control chip 7.

[0041] The microfluidic control chip 7 is provided with a continuous phase inlet capillary channel 1 and a continuous phase outlet capillary channel 2 for continuous phase medium circulation, the continuous phase inlet capillary channel 1 and the continuous phase outlet capillary channel 2 are of the same structure, are of L-shaped structure, and are symmetrically arranged horizontally along the longitudinal center line of the microfluidic control chip 7.

[0042] The microfluidic capillary channel is communicated with a micro-injection pump 6, the micro-injection pump 6 is used for injecting a dispersed phase fluid; the micro-injection pump 6 comprises a first pump body and a micro-injector, the output end of the first pump body is communicated with the input end of the micro-injector, and the output end of the micro-injector is communicated with the microfluidic capillary channel through a capillary tube with an inner diameter of 10 μm.

[0043] The continuous phase inlet capillary channel 1 is communicated with a conventional injection pump 5, and the conventional injection pump 5 is used for injecting a continuous phase fluid. The conventional injection pump 5 comprises a second pump body and an injector, the output end of the second pump body is communicated with the input end of the injector, and the output end of the injector is communicated with the continuous phase inlet capillary channel 1 through a capillary tube with an inner diameter of 500 μm.

[0044] The micro-injection pump 6 communicated with the first microfluidic capillary channel 3 injects the dispersed phase fluid into the microfluidic capillary channel at a preset driving rate and stroke until the dispersed phase fluid fills the microfluidic capillary channel, and the micro-injection pump 6 communicated with the second microfluidic capillary channel 4 injects another dispersed phase fluid into the second microfluidic capillary channel 4 at a preset driving rate and stroke until the dispersed phase fluid fills the microfluidic capillary channel; at this time, the conventional injection pump 5 injects the continuous phase fluid into the continuous phase capillary channel at a preset driving rate and stroke until the continuous phase fluid overflows from above the microfluidic control chip 7 to form a continuous phase environment; at this time, the injection of the dispersed phase fluid by the micro-injection pump 6 is continued until the dispersed phase fluid overflows through the microfluidic capillary channel to form droplets or bubbles.

[0045] In order to adapt to the measurement requirements of the atomic force microscope, the length of the microfluidic control chip 7 is designed to be 11 mm, the width is designed to be 11 mm, and the thickness is designed to be 2 mm; in order to ensure that the size of the prepared droplets and bubbles is within a reasonable range and is convenient for grabbing and fixing, the inner diameter of the microfluidic capillary channel is set to be 10 μm, and the inner diameter of the continuous phase inlet capillary channel 1 and the continuous phase outlet capillary channel 2 is set to be 500 μm; in order to ensure that the fluid can smoothly pass through the microfluidic channel and reduce the frictional resistance, so as to be applicable to the fluid with high viscosity and realize the precise control of the size of the droplets and bubbles, the length of the horizontal part of the microfluidic capillary channel is set to be 5.3 mm, the height of the vertical part is set to be 1 mm, the length of the horizontal part of the continuous phase inlet capillary channel 1 and the continuous phase outlet capillary channel 2 is set to be 4 mm, and the height of the vertical part is set to be 1 mm.

[0046] The application also provides a method for using the microfluid control system, comprising the following steps:

[0047] S1, adjusting the driving rate and stroke, using the microsyringe pump 6 to inject one kind of dispersed phase fluid into the first microfluid capillary channel 3, so that the dispersed phase fluid fills the microfluid capillary channel;

[0048] S2, adjusting the driving rate and stroke, using the microsyringe pump 6 to inject another kind of dispersed phase fluid into the second microfluid capillary channel 4, so that the dispersed phase fluid fills the microfluid capillary channel;

[0049] S3, using the conventional syringe pump 5 to inject the continuous phase fluid into the continuous phase capillary channel, so that the continuous phase fluid fills the continuous phase capillary channel and then overflows from above the microfluid control chip 7, forming a continuous phase environment;

[0050] S4, continuing to inject the dispersed phase fluid, so that the dispersed phase fluid overflows through the microfluid capillary channel to form droplets or bubbles, the diameter of the droplets or bubbles being 50-200 μm, so that the bubbles or droplets can be suitable for atomic force microscope mechanical measurement.

[0051] Wherein, the driving rate and injection volume of the microsyringe pump 6 are set so that one kind of dispersed phase fluid overflows through the first microfluid capillary channel 3 of the microfluid control chip 7 to form droplets or bubbles with a diameter of 50-200 μm; the driving rate and injection volume of the microsyringe pump 6 are set so that another kind of dispersed phase fluid overflows through the second microfluid capillary channel 4 of the microfluid control chip 7 to form droplets or bubbles with a diameter of 50-200 μm.

[0052] Further, S3 is specifically:

[0053] S3, adjusting the driving rate and stroke of the conventional syringe pump 5, using the conventional syringe pump 5 to inject the continuous phase fluid into the continuous phase inlet capillary channel 1, so that the continuous phase fluid fills the continuous phase inlet channel and then overflows from above the microfluid control chip 7, forming a continuous phase environment.

[0054] Further, it also comprises S5;

[0055] S5, opening the continuous phase outlet channel of the microfluid control chip 7, and continuing to use the conventional syringe pump 5 to inject the continuous phase fluid, so that the continuous phase fluid flows out through the continuous phase outlet channel.

[0056] Further, the continuous phase fluid is a liquid or a gas, and the dispersed phase fluid is a liquid or a gas;

[0057] The continuous phase fluid and the dispersed phase fluid are mutually insoluble.

[0058] Further, the injection volume of the dispersed phase fluid is 0.0001 μL to 0.005 μL.

[0059] The above method can be used to prepare water droplets and water droplets, oil droplets and oil droplets, water droplets and oil droplets, water droplets and particles, oil droplets and particles in gas phase, oil droplets and oil droplets, bubbles and bubbles, oil droplets and bubbles, oil droplets and particles, bubbles and particles in water phase, water droplets and water droplets, bubbles and bubbles, water droplets and bubbles, water droplets and particles, bubbles and particles in oil phase, etc.

[0060] The technical solutions of the present application are described below with specific examples, but the protection scope of the present application is not limited by the following examples.

[0061] Example 1

[0062] The present example provides a method for using a microfluidic control system, which comprises the following steps:

[0063] S1: The microsyringe of the microsyringe pump 6 in communication with the first microfluidic capillary channel 3 is filled with a dispersed phase fluid, and the driving rate of the microsyringe pump 6 is set so that the dispersed phase fluid fills the entire capillary and the first microfluidic capillary channel 3 of the microfluidic control chip 7.

[0064] S2: The microsyringe of the microsyringe pump 6 in communication with the second microfluidic capillary channel 4 is filled with another dispersed phase fluid, and the driving rate of the microsyringe pump 6 is set so that the other dispersed phase fluid fills the entire capillary and the second microfluidic capillary channel 4 of the microfluidic control chip 7.

[0065] The dispersed phase fluids in S1 and S2 can be fluids of different physical and chemical properties (including temperature, viscosity, interfacial tension, etc.) but of the same kind, so that the method is also applicable to the preparation of two microdroplets or microbubbles of the same kind with different physical and chemical properties.

[0066] S3: The syringe of the conventional syringe pump 5 is filled with a continuous phase fluid, and the driving rate of the conventional syringe pump 5 is set, and the continuous phase outlet capillary channel 2 of the microfluidic control chip 7 is closed, so that the continuous phase fluid fills the entire capillary and the continuous phase outlet capillary channel 2 of the microfluidic control chip 7 and then overflows from above the microfluidic control chip 7 to form a liquid or gas phase environment of the continuous phase.

[0067] S4: The driving rate and injection volume of the microsyringe pump 6 in communication with the first microfluidic capillary channel 3 are set so that the dispersed phase fluid overflows through the first microfluidic capillary channel 3 of the microfluidic control chip 7 to form droplets or bubbles with a diameter of 50 μm to 200 μm.

[0068] The size of the dispersed phase droplets or bubbles is controlled by setting the injection volume of the microsyringe pump 6.

[0069] S5. Set the driving speed and injection volume of the micro-injection pump 6 connected to the second microfluidic capillary channel 4, so that another dispersed phase fluid overflows through the second microfluidic capillary channel 4 of the microfluidic control chip 7 to form droplets or bubbles with a diameter of 50μm to 200μm.

[0070] The size of the dispersed phase droplets or bubbles can be controlled by setting the injection volume of the micro-injection pump 6, thereby forming dispersed phase monomers of the desired size or a fixed size.

[0071] Furthermore, if it is necessary to displace the continuous phase medium, it also includes S6;

[0072] S6. Open the continuous phase outlet capillary channel 2 of the microfluidic control chip 7, set the driving rate and injection volume of the conventional injection pump 5, so that the continuous phase fluid flows out through the continuous phase outlet capillary channel 2 of the microfluidic control chip 7. This can realize the displacement and flow control of the continuous phase medium, thereby realizing the change of the physicochemical properties of the continuous phase or for analyzing the effect of a constant flow field on dispersed phase droplets or bubbles.

[0073] The rate of continuous phase fluid displacement and the flow field distribution are controlled by setting the driving rate of conventional injection pump 5. If there is no need to displace the continuous phase medium, S6 can be omitted.

[0074] Example 2

[0075] Taking the preparation of n-tetradecane oil droplets and bubbles in water as an example, the usage method of the microfluidic control system is explained in detail. The specific steps are as follows:

[0076] S1. The microsyringe of the microinjection pump 6, which is connected to the first microfluidic capillary channel 3, is filled with n-tetradecane. The driving rate of the microinjection pump 6 is set to 0.001 μL / min, driving the microsyringe so that the n-tetradecane liquid fills the entire capillary and the first microfluidic capillary channel 3 of the microfluidic control chip 7.

[0077] To ensure precise droplet size control, the drive rate of the micro-injection pump 6 should be as low as possible; this parameter is set to the minimum accuracy of the micro-injection pump 6.

[0078] S2. The microsyringe of the microinjection pump 6, which is connected to the second microfluidic capillary channel 4, is filled with air. The driving rate of the microinjection pump 6 is set to 0.001 μL / min, driving the microsyringe so that the air fills the entire capillary and the second microfluidic capillary channel 4 of the microfluidic control chip 7.

[0079] S3, fill the syringe of the conventional syringe pump 5 with deionized water, set the driving rate of the conventional syringe pump 5 as 0.1 mL / min, close the continuous phase outlet capillary channel 2 of the microfluidic control chip 7, drive the syringe so that the deionized water fills the whole capillary and the continuous phase outlet capillary channel 2 of the microfluidic control chip 7 and then overflows from above the chip, forming a water droplet on the chip and covering the first microfluidic channel and the second microfluidic channel.

[0080] S4, set the driving rate of the microsyringe pump 6 communicating with the first microfluidic capillary channel 3 as 0.001 μL / min, and the total injection volume as 0.0005 μL, so that the n-tetradecane forms an oil droplet with a diameter of about 50 μm after overflowing from the first microfluidic capillary channel 3 of the microfluidic control chip 7.

[0081] S5, set the driving rate of the microsyringe pump 6 communicating with the second microfluidic capillary channel 4 as 0.001 μL / min, and the total injection volume as 0.0002 μL, so that the air forms an air bubble with a diameter of about 37 μm after overflowing from the second microfluidic capillary channel 4 of the microfluidic control chip 7.

[0082] As above, the multiphase dispersion system of oil droplets and air bubbles in water can be prepared.

[0083] Further, if the water phase environment is to be changed by displacement, the following steps can be implemented:

[0084] S6, replace the liquid in the syringe with a new aqueous solution, and ensure that no air bubble is formed in the capillary connected with the syringe, open the continuous phase outlet capillary channel 2 of the microfluidic control chip 7, set the driving rate of the conventional syringe pump 5 as 0.1 mL / min and the injection volume as 1 mL, so that the aqueous solution flows out of the continuous phase outlet capillary channel 2 of the microfluidic control chip 7, and the displacement of the aqueous solution can be realized.

[0085] In order to reduce the disturbance of the continuous phase displacement process to the droplets, the driving rate of the conventional syringe pump 5 should be as small as possible, and this parameter is set as the minimum accuracy of the driving of the conventional syringe pump 5.

[0086] The description of each of the above embodiments has its own emphasis, and the parts not described in detail in a certain embodiment can be referred to the relevant description of other embodiments.

[0087] The above only describes the preferred embodiments of the present application and is not used to limit the present application. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application should be included in the protection scope of the present application.

Claims

1. A method of using a microfluidic control system, characterized in that, A microfluidic control system is employed, which includes a microfluidic control chip. The microfluidic control chip is provided with multiple microfluidic capillary channels and a continuous phase capillary channel. The microfluidic capillary channels are connected to a micro-injection pump, and the continuous phase capillary channel is connected to a conventional injection pump. The method of using the microfluidic control system includes the following steps: S1. Adjust the drive speed and stroke, and use a micro-injection pump to inject a dispersed phase fluid into a microfluidic capillary channel so that the dispersed phase fluid fills the microfluidic capillary channel. S2. Adjust the drive speed and stroke, and use a micro-injection pump to inject another dispersed phase fluid into another microfluidic capillary channel so that the dispersed phase fluid fills the microfluidic capillary channel. S3. Use a conventional injection pump to inject the continuous phase fluid into the continuous phase capillary channel, so that the continuous phase fluid fills the continuous phase capillary channel and overflows from the top of the microfluidic control chip to form a continuous phase environment. S4. Continue injecting the dispersed phase fluid, so that the dispersed phase fluid overflows through the microfluidic capillary channel and forms droplets or bubbles with a diameter of 50μm~200μm.

2. The method of using the microfluidic control system as described in claim 1, characterized in that, The inner diameter of the microfluidic capillary channel is 8μm~12μm, and the inner diameter of the continuous phase capillary channel is 480μm~520μm.

3. The method of using the microfluidic control system as described in claim 1, characterized in that, The micro-injection pump includes a pump body and a micro-injector. The output end of the pump body is connected to the input end of the micro-injector, and the output end of the micro-injector is connected to the microfluidic capillary channel through a capillary tube.

4. The method of using the microfluidic control system as described in claim 1, characterized in that, The conventional injection pump includes a pump body and a syringe. The output end of the pump body is connected to the input end of the syringe, and the output end of the syringe is connected to the continuous phase capillary channel through a capillary tube.

5. The method of using the microfluidic control system as described in claim 1, characterized in that, The continuous phase capillary channel includes a continuous phase inlet capillary channel and a continuous phase outlet capillary channel. The continuous phase inlet capillary channel and the continuous phase outlet capillary channel have the same structure and are symmetrically arranged horizontally on the microfluidic control chip along the longitudinal centerline.

6. The method of using the microfluidic control system as described in claim 1, characterized in that, Specifically, S3 is: Close the continuous phase outlet capillary channel on the microfluidic control chip, adjust the drive rate and injection volume of the conventional injection pump, and use the conventional injection pump to inject the continuous phase fluid into the continuous phase inlet capillary channel. After the continuous phase fluid fills the continuous phase inlet channel, it overflows from the top of the microfluidic control chip, forming a continuous phase environment.

7. The method of using the microfluidic control system as described in claim 6, characterized in that, It also includes S5; S5. Open the continuous phase outlet channel on the microfluidic control chip and continue to inject the continuous phase fluid using a conventional syringe pump, so that the continuous phase fluid flows out through the continuous phase outlet channel.

8. The method of using the microfluidic control system as described in claim 1, characterized in that, The continuous phase fluid is a liquid or a gas, and the dispersed phase fluid is a liquid or a gas; The continuous phase fluid and the dispersed phase fluid are immiscible.

9. The method of using the microfluidic control system as described in claim 1, characterized in that, The injection volume of the dispersed phase fluid is 0.0001 μL to 0.005 μL.

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