A kind of fermentation filtrate and its preparation method and application

Abstract

The invention discloses a fermentation filtrate and a preparation method and application thereof, belonging to the technical field of daily chemical products. The preparation method of the invention uses Aspergillus brasiliensis as a fermentation microorganism, and ferments olive oil, which is known as "liquid gold", under a special process. The fermentation degree of macromolecular substances (triglycerides) in the obtained fermentation filtrate is high and the types of active ingredients are rich, and the fermentation filtrate has significant anti-wrinkle, skin tightening and anti-oxidation effects.

Description

Technical Field

[0001] The invention relates to the technical field of daily chemical products, and in particular to a fermentation filtrate and a preparation method and application thereof. Background Art

[0002] There are many types of plant oils used in cosmetics because these natural substances can combine multiple functions such as anti-oxidation, anti-inflammatory, moisturizing and hydrating. The theoretical skin care performance is far better than synthetic chemicals. However, these plant oils are not effective in actual application. The main reason is that most of the active ingredients in plant oils are large molecular substances (triglycerides), which not only make them feel greasy when they come into contact with the skin, but also difficult to spread quickly and be absorbed by the skin, making it difficult to achieve good skin care function.

[0003] Based on this, the existing technology uses fermented products of plant oils as a substitute, using common microorganisms such as bifidobacteria, yeast, and lactobacillus to ferment so that the macromolecules in the plant oils are converted into small molecules. However, the performance of products prepared by different fermentation raw materials and different fermentation processes varies greatly, and some fermented products are even less effective than the original plant oils used alone. Summary of the invention

[0004] Based on the defects of the prior art, the purpose of the present invention is to provide a method for preparing a fermentation filtrate. In this method, Aspergillus brasiliensis is used as a fermentation microorganism, and olive oil, which has the title of "liquid gold", is fermented under a special process. The fermentation degree of macromolecular substances (triglycerides) in the obtained fermentation filtrate is high and the variety of active ingredients is rich, and it has significant anti-wrinkle, skin firming and antioxidant effects.

[0005] In order to achieve the above object, the technical solution adopted by the present invention is:

[0006] A method for preparing a fermentation filtrate comprises the following steps:

[0007] (1) diluting the domesticated Aspergillus brasiliensis to obtain an Aspergillus brasiliensis liquid;

[0008] (2) inoculating a Brazilian Aspergillus liquid into a fermentation substrate, and fermenting at 20 to 30° C. for 36 to 48 hours, separating the resulting mixture, and obtaining a lower filtrate as the fermentation filtrate; the fermentation substrate contains 3 to 10 wt % of emulsified olive oil, and the inoculation amount of the Brazilian Aspergillus liquid is 1 to 3 wt %.

[0009] Olive oil contains a large number of unsaturated oleic acids. Although these oleic acids have excellent skin care functions, they are difficult to achieve the expected effect by direct external application on the skin without any treatment. In the prior art, fermentation microorganisms are used to ferment and degrade them. However, due to the different types of fermentation microorganisms and fermentation processes, the components of the secondary products prepared are also different. In particular, the products after fermentation include aqueous products and oily products. The aqueous products are used to prepare water-phase skin care products, such as lotions, essences, etc., while the oily products are used to prepare oil-phase skin care products, such as creams, etc. However, in general, the skin care functionality of oily products is higher, and the effect of the fermentation filtrate as an aqueous product when used in water-based cosmetics is often not ideal. Therefore, in the technical solution of the present invention, the inventor focuses on improving the functional effect of the fermentation filtrate obtained after the fermentation of olive oil, using Aspergillus braziliensis as the fermentation microorganism, and using emulsified olive oil instead of pure olive oil. Under specific process conditions, Aspergillus braziliensis can be efficiently fermented and obtain a fermentation filtrate with high active ingredients, so that the product has obvious anti-wrinkle, firming and antioxidant effects compared with the olive oil raw material.

[0010] At the same time, the inventors found that the optimal fermentation conditions need to be determined according to the fermentation products. In order to ensure the activity and content of active substances in the fermentation filtrate, the inventors determined the "optimal fermentation temperature and optimal fermentation inoculation amount" corresponding to the fermentation filtrate. The results showed that when the fermentation temperature is too low or the inoculation amount is too small, the total lipase activity in Aspergillus braziliensis is not high. When the fermentation temperature of Aspergillus braziliensis is higher than 30°C, or the inoculation amount is greater than 3wt%, the overall skin care effect of the fermentation filtrate begins to decrease.

[0011] Preferably, the raw materials for preparing the emulsified olive oil include olive oil, polyvinyl alcohol and water.

[0012] More preferably, the raw materials for preparing the emulsified olive oil include olive oil and an aqueous solution of polyvinyl alcohol, the mass ratio of the two is 1:(1-2), and the mass concentration of the aqueous solution of polyvinyl alcohol is 1-3wt%.

[0013] More preferably, the preparation method of the emulsified olive oil is: the components are mixed evenly, and then stirred and dispersed at a rate of 5000 to 8000 rpm for 1 to 2 minutes to obtain the emulsified olive oil.

[0014] If the macromolecular substances (triglycerides) in olive oil are fermented directly, it is difficult to enrich the fermentation conversion products in the fermentation filtrate, and it is difficult to achieve the expected skin care effect. However, the inventors have found that when emulsified olive oil is fermented, the fermentation efficiency of Aspergillus brasiliensis can be effectively improved, and the content of macromolecular conversion substances in the fermentation filtrate is higher.

[0015] Preferably, the domestication comprises the following steps:

[0016] The fermentation substrate is added to Aspergillus brasiliensis for primary acclimation; then the mixture is eluted, and part of the elution product is inoculated into another fermentation substrate for secondary acclimation to obtain Aspergillus brasiliensis liquid.

[0017] More preferably, during the secondary acclimation, the volume ratio of the elution product to the fermentation substrate is (0.5:9.5) to (1.5:8.5).

[0018] Preferably, the fermentation substrate in step (2) comprises the following components by weight: 0.4-0.8 wt% glucose, 1-1.5 wt% peptone, 3-10 wt% olive oil, 0.02-0.1 wt% MgSO 4 , 0.1~0.25wt%K 2 HPO 4 and 0.04-0.05wt% CaCl 2 .

[0019] More preferably, the method for preparing the fermentation substrate comprises the following steps: mixing the components and adjusting the pH of the resulting mixture to 5.5-6.5, followed by heating and sterilization to obtain the fermentation substrate.

[0020] Preferably, the inoculation amount of the Aspergillus brasiliensis liquid is 2-3 wt % (ie, the mass content of the Aspergillus brasiliensis liquid after the Aspergillus brasiliensis liquid is inoculated into the fermentation substrate).

[0021] The lipase activity of Aspergillus brasiliensis in the Aspergillus brasiliensis liquid is affected by the inoculation amount and fermentation conditions. After testing, it was found that when the inoculation amount was selected to be 2-3wt% (especially 3wt%), the lipase activity produced by Aspergillus brasiliensis was higher and more stable, and it could maintain high activity after 48 hours of fermentation, and the fermentation filtrate with the best functional components was obtained.

[0022] Another object of the present invention is to provide a fermentation filtrate prepared by the method for preparing the fermentation filtrate.

[0023] The present invention mainly focuses on the efficacy of aqueous products prepared by fermentation with olive oil as raw material. By replacing pure olive oil with emulsified olive oil, the fermentation filtrate prepared under specific strains and processes is rich in highly active fermentation conversion substances, and has better skin care efficacy than existing products of the same type or products prepared with pure olive oil as fermentation raw material.

[0024] Another object of the present invention is to provide use of the fermentation filtrate in preparing cosmetics.

[0025] A lotion comprises the following components in parts by weight based on 100 parts: 8-12 parts of the fermentation filtrate of the present invention, 0.2-1 part of 1,2-hexanediol, 0.2-1 part of p-hydroxyacetophenone and the balance of water.

[0026] The lotion of the present invention contains fermentation filtrate prepared by a specific process, which can effectively promote the synthesis of type I collagen in the skin after contacting the skin, thereby achieving skin firming, repairing and anti-wrinkle effects. The antioxidant ingredients contained in it can also effectively remove DPPH and ABTS in skin cells, playing an antioxidant effect.

[0027] The beneficial effect of the present invention is that the present invention provides a method for preparing a fermentation filtrate, in which Aspergillus brasiliensis is used as a fermentation microorganism, and olive oil is fermented under a special process. The fermentation degree of macromolecular substances (triglycerides) in the obtained fermentation filtrate is high and the types of active ingredients are rich, and the fermentation filtrate has significant anti-wrinkle, skin tightening and antioxidant effects. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 The HPLC chromatograms of the products of Example 1 (emulsified) and Comparative Example 1 (non-emulsified) in Effect Example 1 of the present invention are shown;

[0029] Figure 2 This is a graph showing the test results of the ability of each test product in Effect Example 2 of the present invention to promote the synthesis of type I collagen in human skin fibroblasts.

[0030] Figure 3 This is a graph showing the antioxidant test results of each test product in Effect Example 3 of the present invention. DETAILED DESCRIPTION

[0031] In order to better illustrate the purpose, technical scheme and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments and comparative examples, the purpose is to understand the content of the present invention in detail, rather than to limit the present invention. All other embodiments obtained by those of ordinary skill in the art without making creative work are within the scope of protection of the present invention. The experimental reagents, raw materials and instruments designed by the present invention and comparative examples are all commonly used ordinary reagents, raw materials and instruments unless otherwise specified. The olive oil described in each embodiment and comparative example is a product produced by NORTHSTAR, UK, and Brazilian Aspergillus is produced by Guangdong Huankai Microbiological Technology Co., Ltd. FSCC114001.

[0032] Example 1

[0033] The method for preparing a fermentation filtrate of the present invention comprises the following steps:

[0034] (1) preparing a fermentation substrate, the fermentation substrate comprising 0.5 wt% glucose, 1.2 wt% peptone, 5 wt% emulsified olive oil, 0.05 wt% MgSO 4 、0.1wt%K 2 HPO 4 、0.05wt%CaCl2 and the balance of water;

[0035] The preparation method of the fermentation substrate is as follows: the components are mixed and the pH is adjusted to 6, followed by heating to 121° C. for sterilization;

[0036] The preparation method of the emulsified olive oil is as follows: olive oil and 2 wt% polyvinyl alcohol solution are mixed in a mass ratio of 1:1.5, and dispersed in a blender at a speed of 6000 rpm for 2 minutes to obtain emulsified olive oil;

[0037] (2) taking part of the fermentation substrate to domesticate Aspergillus brasiliensis to obtain Aspergillus brasiliensis liquid;

[0038] The acclimation step comprises: adding 10 mL of fermentation substrate to the activated Aspergillus brasiliensis in a slant test tube for acclimation, followed by elution and inoculation of 1 mL of the eluted product into another test tube containing 9 mL of the fermentation substrate, mixing and shaking to obtain a Aspergillus brasiliensis liquid;

[0039] (3) inoculating the Aspergillus brasiliensis liquid into the remaining fermentation medium at an inoculum rate of 3 wt % for fermentation, filtering the lower filtrate to obtain a fermentation filtrate; a shaker was used for treatment during the fermentation process, the shaker speed was 250 r / min, the fermentation temperature was set to 30° C., and the fermentation time was 48 h.

[0040] Example 2

[0041] The method for preparing a fermentation filtrate of the present invention is different from that of Example 1 only in that the fermentation substrate comprises the following components by weight: 0.4-0.8 wt% glucose, 1-1.5 wt% peptone, 3-10 wt% olive oil, 0.02-0.1 wt% MgSO 4 , 0.1~0.25wt%K 2 HPO 4 and 0.04-0.05wt% CaCl 2 .

[0042] Comparative Example 1

[0043] A method for preparing a fermentation filtrate, which differs from Example 1 only in that the emulsified olive oil in the fermentation substrate is replaced by a non-emulsified olive oil mixture. The specific preparation method is: olive oil and 2wt% polyvinyl alcohol solution are mixed in a mass ratio of 1:1.5, and manually stirred and dispersed to obtain an olive oil mixture.

[0044] Comparative Example 2

[0045] A method for preparing a fermentation filtrate, which is different from Example 1 only in that the emulsified olive oil in the fermentation substrate is replaced by emulsified rosehip oil. The specific preparation method is: rosehip oil (produced by Granasur SA) and 2wt% polyvinyl alcohol solution are mixed in a mass ratio of 1:1.5, and dispersed in a blender at a speed of 6000 rpm for 2 minutes to obtain emulsified rosehip oil.

[0046] Comparative Example 3

[0047] A method for preparing a fermentation filtrate, the only difference from Example 1 is that the Aspergillus brasiliensis is replaced by Bacillus subtilis GDMCC1.181 produced by Guangdong Provincial Microbial Culture Collection Center.

[0048] Comparative Example 4

[0049] A method for preparing a fermentation filtrate, the only difference from Example 1 is that the Aspergillus brasiliensis is replaced by Lactobacillus plantarum GDMCC1.140 produced by Guangdong Provincial Microbiological Culture Collection Center.

[0050] Comparative Example 5

[0051] A method for preparing a fermentation filtrate, the only difference from Example 1 is that the inoculation amount of the Aspergillus brasiliensis liquid is 5wt%.

[0052] Comparative Example 6

[0053] A method for preparing a fermentation filtrate, the only difference from Example 1 is that the fermentation temperature in step (3) is 35°C.

[0054] Effect Example 1

[0055] In order to verify the conversion efficiency of the macromolecular substances in the fermentation filtrate of the present invention, the product obtained by the preparation method described in Example 1 and the product obtained by the preparation method described in Comparative Example 1 were simultaneously subjected to high performance liquid chromatography analysis, and the corresponding parameters were:

[0056] Chromatographic column: Agilent 5TC-C18(2)250*4.6mm;

[0057] Column temperature: 30°C;

[0058] Detection wavelength: 210nm;

[0059] Mobile phase: acetonitrile:isopropanol = 56:44;

[0060] Flow rate: 1 mL / min;

[0061] Injection volume: 5uL;

[0062] Initial column pressure: 121±10bar.

[0063] The results are as follows Figure 1 As shown, it can be seen that compared with the product described in Comparative Example 1, the content of small molecule substances in the product of Example 1 is higher, indicating that during the fermentation process, the lipase in Aspergillus brasiliensis has a higher response to the emulsified olive oil.

[0064] Effect Example 2

[0065] In order to verify the application effect of the fermentation filtrate of the present invention, the fermentation filtrate obtained in Example 1 was used to prepare lotion according to the following formula:

[0066] 10 parts of fermentation filtrate, 0.5 parts of 1,2-hexanediol, 0.4 parts of p-hydroxyacetophenone, and 89.1 parts of deionized water.

[0067] At the same time, several control groups were set up as follows:

[0068] (1) Control group 1: a lotion prepared according to the same formula, but the fermentation filtrate was replaced by a product prepared by the method of comparative example 1;

[0069] (2) Control group 2: a lotion prepared according to the same formula, but the fermentation filtrate was replaced by the product prepared by the method of comparative example 2;

[0070] (3) Control group 3: a lotion prepared according to the same formula, but the fermentation filtrate was replaced by the product prepared by the method of comparative example 3;

[0071] (4) Control group 4: a lotion prepared according to the same formula, but the fermentation filtrate was replaced by the product prepared by the method of comparative example 4;

[0072] (5) Control group 5: a lotion prepared according to the same formula, but the fermentation filtrate was replaced by the product prepared by the method of comparative example 5;

[0073] (6) Control group 6: a lotion prepared according to the same formula, but the fermentation filtrate was replaced by the product prepared by the method of comparative example 6;

[0074] (7) Control group 7: a lotion prepared according to the same recipe, but the fermentation filtrate was replaced by the filtrate of the bifid yeast fermentation product produced by Modern Biolande Biotechnology (Jiangsu) Co., Ltd.;

[0075] (8) Control group 8: a lotion prepared according to the same recipe, but the fermentation filtrate was replaced by the bifida yeast fermentation product lysate produced by Modern Biolande Biotechnology (Jiangsu) Co., Ltd.;

[0076] The essence water prepared from the product of Example 1 and the products of control groups 1 to 8 were tested for their ability to promote the synthesis of type I collagen in human skin fibroblasts. The specific method is as follows:

[0077] Cell digestion and inoculation: Human skin fibroblasts purchased from the market were digested with trypsin / EDTA. When most of the cells became round and in suspension, serum-containing DMEM medium with a volume of about 2 times that of trypsin was added to terminate the digestion, and the cells were collected in a centrifuge tube and centrifuged at 1000 rpm for 5 minutes. After centrifugation, the supernatant was discarded, and cell culture medium was added to the centrifuge tube for resuspending, and the cell concentration was adjusted to the inoculation density (the degree of confluence reached 45% to 60% 24 hours after inoculation), and then inoculated into a 96-well plate, 100 μL per well, and placed in a CO 2 Culture in an incubator for 24 h ± 2 h.

[0078] Administration: Discard the medium in the 96-well plate, dilute the test substance to 1% with cell culture medium and add it to the test substance wells, add culture medium containing 100 ng / mL TGFβ1 to the positive control wells, and add cell culture medium to the blank control wells, 100 μL per well. After administration, place the 96-well plate in a CO 2 Culture in an incubator for 24 h ± 2 h.

[0079] ELISA detection: After the culture is completed, the cell culture supernatant in each well is collected and detected according to the instruction manual of the human type I collagen enzyme-linked immunosorbent assay kit.

[0080] Calculation of type I collagen content: Use professional curve making software to calculate the regression equation of the standard curve using the concentration of the standard in the kit and the OD450 value, substitute the OD450 value of the sample into the equation, and calculate the type I collagen content of the sample. The average value of the three replicate wells in each group is used as the final type I collagen result.

[0081] The calculation formula for the up-regulation rate of type I collagen is: up-regulation rate (%) = (T / C-1) × 100%, where: T is the average value of type I collagen content in the test substance; C is the average value of type I collagen content in the blank control.

[0082] Test results such as Figure 2 As shown, the NC group and the PC group are also included in this figure.

[0083] from Figure 2It can be seen that the fermentation filtrate of the present invention can effectively promote the synthesis of type I collagen in human skin fibroblasts when applied to the skin. The upregulation rate of type I collagen compared to the control group is as high as nearly 400%, which can effectively achieve the effect of firming the skin and anti-wrinkle. However, the control group products prepared by the comparative products did not meet expectations. The fermentation filtrate products prepared by unemulsified olive oil or emulsified rosehip oil did not have obvious effects in promoting type I collagen, and the fermentation filtrate prepared by other common types of fermentation microorganisms and existing commercial fermentations also did not have this biological activity. Although the control group products 5 and 6 were prepared with the same fermentation raw materials, the process conditions during the preparation process were not ideal, and the resulting fermentation filtrates also did not achieve the expected skin firming and anti-wrinkle effects.

[0084] Furthermore, each product was subjected to an antioxidant test, and the test method was as follows:

[0085] DPPH free radical scavenging method:

[0086] Preparation of the sample solution to be tested: Weigh 10.0 mg of the sample, dissolve it in distilled water, make it up to 1.0 mL, mix it thoroughly, and prepare a 10.0 mg / mL mother solution. Dilute the mother solution with distilled water to different multiples to obtain sample solutions of different concentrations.

[0087] Preparation of glutathione solution: Weigh 10.0 mg of L-reduced glutathione, dilute to 1.0 mL with distilled water, mix thoroughly, and prepare a 10.0 mg / mL mother solution. Dilute the mother solution with distilled water to different multiples to obtain glutathione solutions of different concentrations.

[0088] Determination:

[0089] Control group: Take 3 test tubes, numbered 1, 2, and 3, add 3.0mL DPPH solution and 1.0mL glutathione solution to test tube 1 as the test group (As); add 1.0mL glutathione solution and 3.0 anhydrous ethanol solution to test tube 2 as the control group (Ac); add 3.0mL DPPH solution and 1.0mL sample solvent solution to test tube 3 as the blank group (Ab). After fully mixing, react at room temperature in the dark for 30 minutes, and measure the absorbance value with a UV spectrophotometer at a wavelength of 517nm (sample solvent zero calibration).

[0090] Sample group: The glutathione solution was replaced by the sample solution to be tested, and other operations were the same as the control group.

[0091] Experimental data processing:

[0092] P=[1-(A s -A c ) / Ab]×100%

[0093] Where: P—clearance rate; A s —The absorbance of the mixture of the test solution and the DPPH solution; A c —The absorbance of the mixture of the test solution and the anhydrous ethanol solution; Ab—The absorbance of the mixture of the DPPH solution and the sample solvent solution.

[0094] ABTS+ free radical scavenging method:

[0095] Preparation of sample solution: Weigh 10.0 mg of sample, dissolve it in distilled water and dilute to 1.0 mL, mix thoroughly to prepare a 10.0 mg / mL stock solution. Dilute the stock solution to different multiples with 95% ethanol to obtain solutions of different concentrations.

[0096] Preparation of glutathione solution: Weigh 10.0 mg of L-reduced glutathione, dilute to 1.0 mL with distilled water, mix thoroughly to prepare a 10.0 mg / mL mother solution. Dilute the mother solution to different multiples with 95% ethanol to obtain different concentrations of test solutions.

[0097] Determination:

[0098] Control group: Take 2 test tubes, numbered 1 and 2, add 3.6mL ABTS solution and 0.4mL glutathione solution to test tube 1 as the experimental group (A s ); add 3.6mL ABTS solution and 0.4mL sample solvent solution into test tube No. 2 as blank group (A b ); after being fully mixed, react at room temperature in the dark for 5 minutes, and measure the absorbance value with an ultraviolet spectrophotometer at a wavelength of 734 nm (sample solvent zero calibration).

[0099] Sample group: The glutathione solution was replaced by the sample solution to be tested, and other operations were the same as the control group.

[0100] Experimental data processing: P = (A b -A s ) / Ab×100%

[0101] Where: P—clearance rate; A b —Absorbance of the mixture of ABTS solution and sample solvent solution; A s —The absorbance of the mixture of the test solution and ABTS solution.

[0102] Test results such as Figure 3 shown.

[0103] It can be seen that the lotion product prepared by the fermentation filtrate of the present invention has a uniformly high scavenging ability for ABTS and DPPH, which is much better than the control groups 7 and 8 prepared by the existing commercial products of the same type. In contrast, although the fermentation filtrate prepared by non-emulsified olive oil and emulsified rosehip oil also has a certain antioxidant effect, the effect is not as good as that of the product in Example 1. At the same time, the antioxidant effect of the products prepared by other microbial fermentation is also poor.

[0104] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present invention.

Claims

1. A method for preparing a fermentation filtrate, characterized in that: The following steps are involved: (1) diluting the domesticated Aspergillus brasiliensis to obtain Aspergillus brasiliensis liquid; Aspergillus brasiliensis is FSCC114001 produced by Guangdong Huankai Microbiological Technology Co., Ltd.; (2) inoculating a Brazilian Aspergillus liquid into a fermentation substrate, and fermenting at 20 to 30° C. for 36 to 48 hours, separating the resulting mixture, and obtaining a lower filtrate as the fermentation filtrate; the fermentation substrate contains 3 to 10 wt % of emulsified olive oil, and the inoculation amount of the Brazilian Aspergillus liquid is 1 to 3 wt %.

2. The method for preparing the fermentation filtrate according to claim 1, characterized in that: The raw materials for preparing the emulsified olive oil include olive oil, polyvinyl alcohol and water.

3. The method for preparing the fermentation filtrate according to claim 2, characterized in that: The raw materials for preparing the emulsified olive oil include olive oil and an aqueous solution of polyvinyl alcohol, the mass ratio of the two is 1:(1-2), and the mass concentration of the aqueous solution of polyvinyl alcohol is 1-3wt%.

4. The method for preparing the fermentation filtrate according to claim 3, characterized in that: The preparation method of the emulsified olive oil is as follows: the components are mixed uniformly, and then stirred and dispersed at a speed of 5000 to 8000 rpm for 1 to 2 minutes to obtain the emulsified olive oil.

5. The method for preparing the fermentation filtrate according to claim 1, characterized in that: The domestication The following steps are involved: The fermentation substrate is added to Aspergillus brasiliensis for primary acclimation; then the mixture is eluted, and part of the elution product is inoculated into another fermentation substrate for secondary acclimation to obtain Aspergillus brasiliensis liquid.

6. The method for preparing the fermentation filtrate according to claim 5, characterized in that: During the secondary acclimation, the volume ratio of the eluted product to the fermentation substrate is (0.5:9.5) to (1.5:8.5).

7. The method for preparing the fermentation filtrate according to claim 1, characterized in that: The fermentation substrate in step (2) includes the following components in mass content: 0.4-0.8wt% glucose, 1-1.5wt% peptone, 3-10wt% emulsified olive oil, 0.02-0.1wt% MgSO4, 0.1-0.25wt% K2HPO4 and 0.04-0.05wt% CaCl2.

8. The fermentation filtrate prepared by the method for preparing the fermentation filtrate according to any one of claims 1 to 7.

9. Use of the fermentation filtrate according to claim 8 in the preparation of cosmetics.

10. A lotion, characterized in that: The composition comprises the following components in parts by weight based on 100 parts: 8 to 12 parts of the fermentation filtrate according to claim 8, 0.2 to 1 part of 1,2-hexanediol, 0.2 to 1 part of p-hydroxyacetophenone and the balance of water.

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