Cosmetic composition comprising a mixed extract of ivy, plantain, mallow and okra and a method for its preparation

Abstract

The present invention relates to a cosmetic composition for achieving anti-inflammatory and moisturizing effects by containing a mixed extract of mistletoe, plantain, mallow, and okra as an effective ingredient, and a preparation method thereof.

Description

TECHNICAL FIELD

[0001] The present application relates to a cosmetic composition and a method of preparing the same, and more particularly, to a cosmetic composition comprising a mixed extract of ivy, plantain, mallow, and okra as an active ingredient, which achieves anti-inflammatory and moisturizing effects, and a method of preparing the same. BACKGROUND

[0002] Skin is generally divided into three layers of epidermis, dermis, and subcutaneous fat tissue from the outside, and has a function of protecting the human body from physical and chemical stimuli from the outside environment, and particularly, the skin controls about 65 to 70% of water contained in the human body to evaporate to the outside.

[0003] Among them, the epidermis is divided into the horny layer, the granular layer, the prickle layer, and the basal layer from the outside, and the horny layer of the epidermis contains about 10 to 20% of water, which exists at the outermost side of the human body to inhibit the evaporation of water from the body while blocking the excessive penetration of external substances (Journal of Investigative Dermatology, J. Invest. Dermatol. 80 (Suppl.), 44-49. 1983).

[0004] A high concentration of natural moisturizing factor (NMF) as a water-soluble component exists in the cells constituting such a horny layer, which helps the skin to exhibit elasticity and maintain proper moisture (Journal of Investigative Dermatology, J. Invest. Dermatol. 54, 24-31, 1970).

[0005] However, due to changes in the environment or lifestyle, various stresses and environmental pollution in social life, frequent face washing based on cosmetic habits, and natural aging of the skin as age increases, and the like, the water in the horny layer is reduced and causes the skin to become dry, the skin surface to become rough, the skin to lose luster and appear dull, and the like, and thus the importance of the skin moisturizing problem is increasing.

[0006] In this regard, various cosmetic raw materials for the purpose of improving skin moisturizing are being developed, and from the recent external environment, the use amount of animal raw materials has sharply decreased due to the decrease in the preference of consumers for animal raw materials and the uproar over mad cow disease, and raw materials derived from natural plants are attracting attention.

[0007] On the other hand, inflammation, which is one of the innate immunity, is a homeostatic response that protects and maintains our body from viral or bacterial infection. However, it is well known that excessive acute inflammation and chronic inflammatory responses are major causes of cancer, diabetes, and vascular-related diseases, which are the three major causes of death. Specifically, it is well known that metabolic diseases, vascular-related diseases, autoimmune diseases, nervous system diseases, musculoskeletal system diseases, and cancer, etc. are caused by various pathological processes (generation of free radicals, generation of inflammatory cytokines, generation of chemotactic factors, etc.) caused by persistent inflammatory states. Therefore, if the inflammatory response can be properly and effectively controlled and treated, it is believed that the diseases as the above major causes of death can be sufficiently reduced. In this regard, there is a need for a substance that can safely and effectively inhibit the inflammatory response.

[0008] To solve this problem, the existing technology mainly attempts to solve the problem as described above by a chemical ingredient. However, there is a problem that cosmetics using a chemical ingredient can cause side effects and are not sustainable in ecology. Therefore, research is being conducted on cosmetic raw materials that combine various natural plants.

[0009] Prior Art Documents

[0010] Patent Documents

[0011] Patent Document 0001: Korean Patent No. 10-2348707 (Publication Date: January 6, 2022), "Cosmetic or pharmaceutical composition for skin elasticity, wrinkle improvement, moisturizing, or anti-inflammation containing docetaxel or a pharmaceutically acceptable salt thereof" SUMMARY

[0012] Technical Problem to be Solved

[0013] To solve the problem as described above, the present application aims to provide a cosmetic composition containing a mixed extract of ivy, plantain, mallow, and okra as an active ingredient, which achieves anti-inflammatory and moisturizing effects by containing a mixed extract of ivy, plantain, mallow, and okra as an active ingredient, and a method for preparing the same.

[0014] Technical Solution to Solve the Problem

[0015] To achieve the object as described above, the present application provides a cosmetic composition containing a mixed extract of ivy, plantain, mallow, and okra as an active ingredient, characterized by comprising a mixed extract extracted from a mixture of ivy, plantain, mallow, and okra.

[0016] Furthermore, the present invention is characterized in that the above mixture is mixed with mistletoe, plantain, mallow and okra in a weight ratio of 0.1-2.5:0.1-2.5:0.1-2.5:0.1-2.5 respectively.

[0017] Furthermore, the present invention is characterized in that the above-mentioned mixed extract is extracted using any one of the following extraction solvents selected from the group consisting of purified water, anhydrous or aqueous lower alcohols having a carbon number of 1 to 4, acetone, ethyl acetate, butyl acetate, and 1,3-butanediol.

[0018] Furthermore, the present invention is characterized in that, after cooling the above-mentioned mixed extract to room temperature, it is filtered with a filter cloth and concentrated under reduced pressure.

[0019] Furthermore, the present invention provides a method for preparing a cosmetic composition comprising a mixed extract of mistletoe, plantain, mallow, and okra as an active ingredient, comprising: a mixture preparation step S10, wherein mistletoe, plantain, mallow, and okra are mixed to prepare a mixture; an extraction step S20, wherein the mixture prepared in the mixture preparation step S10 is mixed with any one of the following extraction solvents selected from the group consisting of purified water, anhydrous or aqueous lower alcohols having a carbon number of 1 to 4, acetone, ethyl acetate, butyl acetate, and 1,3-butanediol to extract the mixed extract; and a filtration and concentration step S30, wherein the mixed extract in the extraction step S20 is cooled, filtered, and concentrated under reduced pressure.

[0020] Furthermore, in the extraction step S20 described above, the mixture prepared in the mixture preparation step S10 and the extraction solvent are mixed at a weight ratio of 0.1 to 2.0: 10.0 to 30.0, and extracted at a temperature of 80 to 100°C for 2 to 6 hours.

[0021] Furthermore, in the above-mentioned filtration and concentration step S30, the mixed extract from the above-mentioned extraction step S20 is cooled at room temperature, and then filtered with a 200-600 mesh filter cloth and concentrated under reduced pressure.

[0022] Beneficial effects of the invention

[0023] The cosmetic composition prepared according to the method of the present invention contains a mixed extract of mistletoe, plantain, mallow and okra as an active ingredient, and achieves anti-inflammatory and moisturizing effects by including the mixed extract of mistletoe, plantain, mallow and okra as an active ingredient. Attached Figure Description

[0024] Figure 1 The flowchart illustrates the preparation process of a cosmetic composition comprising a mixed extract of mistletoe, plantain, mallow, and okra as an active ingredient according to the present invention.

[0025] Figure 2A bar chart for the anti-inflammatory activity evaluation regarding Preparation Example 1 to Preparation Example 5.

[0026] Figure 3 A bar chart for the anti-inflammatory activity evaluation regarding Preparation Example 1 to Preparation Example 5.

[0027] Figure 4 A bar chart for the collagen synthesis promotion efficacy regarding Preparation Example 1 to Preparation Example 5.

[0028] Figure 5 A bar chart for the skin moisture improvement effect regarding Example 1 and Comparative Example 1 to Comparative Example 4.

[0029] Figure 6 A bar chart for the transepidermal water loss regarding Example 1 and Comparative Example 1 to Comparative Example 4.

[0030] Explanation of reference numerals

[0031] S10: mixture preparation step

[0032] S20: extraction step

[0033] S30: filtration and concentration step DETAILED DESCRIPTION

[0034] The following detailed description of the present application is an embodiment in which the present application can be implemented, and will be described with reference to the accompanying drawings, which are shown as examples of the corresponding embodiment. The following will be described in detail so that those skilled in the art can fully implement the present application. It should be understood that although various embodiments of the present application are different from each other, they do not need to be mutually exclusive. For example, the specific shape, structure, and characteristics described herein with respect to one embodiment can be embodied in other embodiments without departing from the spirit and scope of the present application. Also, it should be understood that the position or arrangement of individual structural elements within each embodiment described can be modified without departing from the spirit and scope of the present application.

[0035] Therefore, the following detailed description is not intended to be limiting in nature, and if detailed description is obtained, the scope of the present application is limited only to all ranges equivalent to the content claimed by the claims thereof and the attached claims. Similar reference numerals in the drawings represent the same or similar functions at multiple levels.

[0036] The terms used in the present application are selected, as far as possible, from general terms that are widely used at present in consideration of functions in the present application, but can be changed along with the intention or precedent of those skilled in the art, appearance of new technology, etc. Also, in specific cases, there are terms arbitrarily selected by the applicant, which are detailed in the specification part of the corresponding application. Therefore, the terms used in the present application should be defined based on the meaning of the terms and the overall contents of the present application, not the name of the terms themselves.

[0037] In the present application, when a certain part "comprises" a certain structural element, unless otherwise specifically recited, it means that other structural elements can also be included, not excluding other structural elements.

[0038] Hereinafter, a cosmetic composition containing a mixed extract of mistletoe, plantain, mallow, and cosmos as an effective ingredient and a method of preparing the same will be described in detail.

[0039] The cosmetic composition of the present application is characterized by containing a mixed extract of mistletoe, plantain, mallow, and cosmos as an effective ingredient.

[0040] The above-mentioned mistletoe (Viscum album var. lutescens) is a parasitic evergreen shrub of the family Loranthaceae in the order Santalales of dicotyledon, which parasitizes on oak, alder, chestnut, and catalpa. It grows in a round shape like a bird's nest, and also has a diameter of up to 1 m. The leaves are opposite, thick leathery, and lanceolate, and have no petiole. The branches are cylindrical and yellow-green, and the internodes of the twigs are 3 to 6 cm long.

[0041] The above-mentioned plantain (Plantago asiatica) is a perennial plant of the family Plantaginaceae in the order Plantaginales of dicotyledon, which grows in grasslands, roadsides, or open spaces. It has no stem, and the leaves are clustered from the root, are oval or egg-shaped, are 4 to 15 cm long, and are 3 to 8 cm wide. The 5 parallel leaf veins are clear, and the edges have wavy serrations. The petiole length is similar to that of the blade, and the base is enlarged into a sheath.

[0042] The above-mentioned mallow (Malva sylvestris var. mauritiana) is a biennial plant of the family Malvaceae in the order Malvales of dicotyledon, also known as Malva sylvestris. It is originally from Asia, and is 60 to 90 cm high. The leaves are alternate, are round but are divided into 5 to 9 lobes, and have small serrations on the edges. The leaf base is heart-shaped.

[0043] The above-mentioned Hibiscus esculentus is a dicotyledonous plant of the Malvales order of the Malvaceae family, and is a perennial plant native to northeast Africa, and has been cultivated as a vegetable in Egypt since about 2000 years ago. The stem is erect and grows to a height of 50 to 200 cm. The leaves are alternate, petioled, cordate, and pinnately divided into 3 to 9 lobes with serrated edges. The flowers are yellow and bloom from summer to autumn, and are open at night and closed the next morning, similar to cotton. The part that encloses the seeds grows rapidly, and thus can be harvested the next night.

[0044] Preferably, the composition of the present application comprises a mixed extract obtained by extracting a mixture of Torix, Plantago, Malva, and Hibiscus esculentus mixed at a weight ratio of 0.1 to 2.5:0.1 to 2.5:0.1 to 2.5:0.1 to 2.5, and more specifically, preferably, Torix, Plantago, Malva, and Hibiscus esculentus are mixed at a weight ratio of 1:1:1:1 and extracted.

[0045] In this case, it is suitable to extract the mixture of Torix, Plantago, Malva, and Hibiscus esculentus with any one selected from the group consisting of purified water, anhydrous or hydrous lower alcohols having a carbon number of 1 to 4, acetone, ethyl acetate, butyl acetate, and 1,3-butanediol as an extraction solvent, and more specifically, preferably, purified water.

[0046] On the other hand, in the composition of the present application, the mixture of Torix, Plantago, Malva, and Hibiscus esculentus can be extracted with an extraction solvent mixed at a weight ratio of 0.1 to 2.0:10.0 to 30.0, and more specifically, most preferably, the mixture of Torix, Plantago, Malva, and Hibiscus esculentus is mixed with an extraction solvent at a weight ratio of 1.0:20.0 and extracted.

[0047] Also, in the composition of the present application, when the mixture of Torix, Plantago, Malva, and Hibiscus esculentus is extracted with an extraction solvent, it is preferably extracted at a temperature of 80 to 100°C for 2 to 6 hours, and more specifically, most preferably, the mixture of Torix, Plantago, Malva, and Hibiscus esculentus can be mixed with an extraction solvent and extracted at a temperature of 90°C for 4 hours.

[0048] On the other hand, preferably, the composition of the present application extracts the mixture of Torix, Plantago, Malva, and Hibiscus esculentus with an extraction solvent, and after cooling to room temperature, filters it with a filter cloth having a mesh of 200 to 600 and concentrates it under reduced pressure.

[0049] The composition of the present application can be obtained by mixing the mixture of the mistletoe, the plantain, the mallow, and the sunhemp in the weight ratio of 0.1 to 2.5:0.1 to 2.5:0.1 to 2.5:0.1 to 2.5 with any one extraction solvent selected from the group consisting of purified water, anhydrous or hydrous lower alcohol having a carbon number of 1 to 4, acetone, ethyl acetate, butyl acetate, and 1,3-butanediol, and extracting at a temperature of 80 to 100°C for 2 to 6 hours, filtering with a filter cloth of 200 to 600 meshes after cooling to room temperature, and concentrating under reduced pressure.

[0050] Most suitably, the mixture of the mistletoe, the plantain, the mallow, and the sunhemp in the weight ratio of 1:1:1:1 is mixed with the purified water in the weight ratio of 1:20, and extracted at a temperature of 90°C for 4 hours, filtered with a filter cloth of 400 meshes after cooling to room temperature, and concentrated under reduced pressure.

[0051] In addition to the composition of the present application, the present application includes ingredients generally used in cosmetic compositions, for example, general adjuvants such as antioxidants, stabilizers, co-solvents, vitamins, pigments, colorants, and fragrances, and carriers.

[0052] The cosmetic composition of the present application can be manufactured in all dosage forms generally manufactured in the art, for example, can be manufactured in a solution, a suspension, an emulsion, a paste, a gel, a cream, a liquid, a powder, a soap, a surfactant-containing cleanser, an oil, a powdery foundation, an emulsion-type foundation, a wax-type foundation, a spray, and the like, but is not limited thereto. In more detail, a softening toner, a nourishing toner, a nourishing cream, a massage cream, an essence, a pack, an eye cream, a makeup remover, a cleansing foam, a makeup remover, a pack, a spray, or a powder dosage form can be manufactured.

[0053] In the case where the dosage form of the present application is a paste, a cream, or a gel, one or more selected from among animal oil, vegetable oil, wax, paraffin, starch, tragacanth gum, cellulose derivatives, polyethylene glycol, silicone, bentonite, silica, talc, or zinc oxide can be used as a carrier ingredient.

[0054] In the case where the dosage form of the present application is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder can be used as a carrier ingredient, and especially in the case of a spray, a propellant such as chlorofluorocarbon, propane / butane, or dimethyl ether can be additionally included.

[0055] In the case where the dosage form of the present application is a solution or an emulsion, a solvent, a solubilizing agent or an emulsifying agent is used as a carrier ingredient, and for example, one or more selected from the group consisting of water, ethanol, isopropyl alcohol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, butylene glycol, 1,3-butylene glycol oil, polyoxyethylene hydrogenated castor oil, glycerol, glycerin, fatty ester, phenoxyethanol, triethanolamine, polyethylene glycol, beeswax, polysorbate 60, sorbitan sesquioleate, paraffin, sorbitan stearate, lipophilic glyceryl monostearate, stearic acid, glyceryl stearate / polyethylene glycol 400 stearate, carboxy polymer, sitosterol, polyglyceryl 2-oleate, ceramide, cholesterol, Steareth-4, dicetyl phosphate, macadamia nut oil, carboxyvinyl polymer, xanthan gum, or sorbitan fatty acid ester can be used.

[0056] In the case where the dosage form of the present application is a suspension, one or more selected from the group consisting of a liquid diluent such as water, ethanol, glycerol, butylene glycol or propylene glycol; a suspension such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester; microcrystalline cellulose, hydroxyethyl cellulose, sodium hyaluronate, phenoxyethanol, aluminum metahydroxide, bentonite, stearic acid, cetyl alcohol, glyceryl monostearate, polyoxyethylene sorbitan monostearate, sorbitan sesquioleate, glyceryl monostearate / glyceryl stearate / polyoxyethylene stearate, wax, paraffin, squalane, caprylic / capric triglyceride, carboxyvinyl polymer, triethanolamine, agar, or tragacanth can be used as a carrier ingredient.

[0057] In the case where the dosage form of the present application is a surfactant-containing cleanser, one or more selected from the group consisting of fatty alcohol sulfate, fatty alcohol ether sulfate, sulfosuccinic monoester, isethionate, imidazoline derivative, methyl taurate, sarcosinate, fatty acid amide ether sulfate, cocamidopropyl betaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester can be used as a carrier ingredient.

[0058] Also, the use efficacy of the cosmetic composition of one embodiment of the present application can be appropriately adjusted according to the skin state and condition of the individual, and external environmental conditions.

[0059] Hereinafter, a method of preparing the cosmetic composition of the present application containing the mixed extract of ivy, plantain, mallow, and okra as an effective ingredient will be described in detail.

[0060] The preparation method of the cosmetic composition of the present application including the mixed extract of mistletoe, plantain, mallow, and sunflower as an effective ingredient includes: a mixture preparation step S10 of preparing a mixture by mixing mistletoe, plantain, mallow, and sunflower; an extraction step S20 of extracting a mixed extract by mixing the mixture prepared in the mixture preparation step S10 with any one extraction solvent selected from the group consisting of purified water, anhydrous or aqueous lower alcohol having a carbon number of 1 to 4, acetone, ethyl acetate, butyl acetate, and 1,3-butanediol; and a filtration and concentration step S30 of cooling, filtering, and concentrating under reduced pressure the mixed extract in the extraction step S20.

[0061] First, the mixture preparation step S10 is performed.

[0062] In the mixture preparation step S10, the mixture is prepared by mixing mistletoe, plantain, mallow, and sunflower.

[0063] Preferably, the mixture in the mixture preparation step S10 is mixed with mistletoe, plantain, mallow, and sunflower at a weight ratio of 0.1 to 2.5: 0.1 to 2.5: 0.1 to 2.5: 0.1 to 2.5, respectively.

[0064] More specifically, preferably, the mixture in the mixture preparation step S10 is mixed with mistletoe, plantain, mallow, and sunflower at a weight ratio of 1: 1: 1: 1, respectively.

[0065] Then, the extraction step S20 is performed.

[0066] In the extraction step S20, the mixture prepared in the mixture preparation step S10 is mixed with any one extraction solvent selected from the group consisting of purified water, anhydrous or aqueous lower alcohol having a carbon number of 1 to 4, acetone, ethyl acetate, butyl acetate, and 1,3-butanediol, and is extracted to prepare a mixed extract.

[0067] In the extraction step S20, the mixture prepared in the mixture preparation step S10 is mixed with any one extraction solvent selected from the group consisting of purified water, anhydrous or aqueous lower alcohol having a carbon number of 1 to 4, acetone, ethyl acetate, butyl acetate, and 1,3-butanediol at a weight ratio of 0.1 to 2.0: 10.0 to 30.0, and is extracted at a temperature of 80 to 100°C for 2 to 6 hours.

[0068] More specifically, preferably, in the extraction step S20, the mixture prepared in the mixture preparation step S10 is mixed with purified water at a weight ratio of 1:20, and is extracted at a temperature of 90°C for 4 hours.

[0069] Then, the filtration and concentration step S30 is performed.

[0070] In the filtration and concentration step S30, the mixed extract of the extraction step S20 is cooled, filtered, and concentrated under reduced pressure.

[0071] In the filtration and concentration step S30, the mixed extract of the extraction step S20 is cooled at room temperature, then filtered with a filter cloth of 200 to 600 mesh and concentrated under reduced pressure.

[0072] More specifically, preferably, in the filtration and concentration step S30, the mixed extract of the extraction step S20 is cooled at room temperature, then filtered with a filter cloth of 400 mesh and concentrated under reduced pressure.

[0073] Hereinafter, the effects of the cosmetic composition of the present application will be explained in detail through the following Preparation Examples, Examples, Comparative Examples, and Experimental Examples.

[0074] Preparation Example 1. Preparation of the mixed extract of the present application

[0075] A mixture of the mistletoe, the plantain, the mallow, and the zucchini mixed in a weight ratio of 1:1:1:1 was mixed with purified water in a weight ratio of 1:20, and extracted at a temperature of 90°C for 4 hours, and after cooling to room temperature, filtered with a filter cloth of 400 mesh and concentrated under reduced pressure.

[0076] Preparation Example 2. Preparation of the extract of the mistletoe alone

[0077] The mistletoe was mixed with purified water in a weight ratio of 1:20, and extracted at a temperature of 90°C for 4 hours, and after cooling to room temperature, filtered with a filter cloth of 400 mesh and concentrated under reduced pressure.

[0078] Preparation Example 3. Preparation of the extract of the plantain alone

[0079] The plantain was mixed with purified water in a weight ratio of 1:20, and extracted at a temperature of 90°C for 4 hours, and after cooling to room temperature, filtered with a filter cloth of 400 mesh and concentrated under reduced pressure.

[0080] Preparation Example 4. Preparation of the extract of the mallow alone

[0081] The mallow was mixed with purified water in a weight ratio of 1:20, and extracted at a temperature of 90°C for 4 hours, and after cooling to room temperature, filtered with a filter cloth of 400 mesh and concentrated under reduced pressure.

[0082] Preparation Example 5. Preparation of the extract of the zucchini alone

[0083] The zucchini was mixed with purified water in a weight ratio of 1:20, and extracted at a temperature of 90°C for 4 hours, and after cooling to room temperature, filtered with a filter cloth of 400 mesh and concentrated under reduced pressure.

[0084] Example 1 and Comparative Examples 1 to 4. Preparation of dosage forms using the preparations of Preparation Examples 1 to 5

[0085] As shown in Table 1 below, Example 1 and Comparative Examples 1 to 4 were prepared by formulating the preparations of Preparation Examples 1 to 5 described above.

[0086] Table 1

[0087]

[0088]

[0089] Experimental Example 1. Anti-inflammatory activity evaluation (1) - Nitric Oxide inhibition assay

[0090] The anti-inflammatory activity of the samples obtained from the above Preparation Examples 1 to 5 was measured. After suspending the samples obtained from the above Preparation Examples 1 to 5 in purified water to form different concentrations (0.1%, 0.5%, 1%, 3%, 5%), the anti-inflammatory activity was measured by the following method.

[0091] 1) Experimental method

[0092] Raw 264.7 cells, which are mouse macrophages, were inoculated into a 6-well plate at a concentration of 3.5 x 10 5 cells / mL and cultured for 24 hours. Thereafter, the cell culture medium containing the sample prepared in Preparation Examples 1 to 5 was replaced in each well. 1 μg of lipopolysaccharide (LPS) was treated as a stimulant and cultured for 24 hours. 100 μL of the supernatant was taken out and transferred to a 96-well plate, 100 μL of Griess solution was added and reacted at room temperature for 10 minutes, and then the absorbance was measured at 540 nm to determine the nitric oxide (NO) inhibition effect, the results of which are shown in Table 2 below.

[0093] Table 2

[0094]

[0095]

[0096] The experimental results, as shown in Table 2 and Figure 2 , can know that the nitric oxide (NO) inhibition effect of the different treatment concentrations of Preparation Example 1 containing the mixed extract of the present application is the most excellent compared to Preparation Examples 2 to 5.

[0097] Experimental Example 2. Anti-inflammatory activity evaluation (2) - β-Hexosaminidase release

[0098] The anti-inflammatory activity of the immune cells of Preparation Examples 1 to 5 above was measured.

[0099] 1) Experimental method

[0100] The RBL-2H3 cell line was cultured in a Dulbeccos' modified Eagle's medium (hereinafter DMEM) containing 1% fetal bovine serum and L-glutamine at a temperature of 37°C in a 5% carbon dioxide (CO2) incubator, and the adherent cells were suspended and isolated by using a Trypsin-ethylenediaminetetraacetic acid (EDTA) solution and recovered for use in the experiment.

[0101] After the RBL-2H3 cell line was activated with an active factor (dinitrophenol-conjugated human serum albumin (DNP-HSA)-specific IgE), the activated cells were treated with an antigen (DNP-HSA) at 50 ng / mL, and each extract was treated at an amount of 5 μg / mL, 25 μg / mL, and 125 μg / mL and cultured for 60 minutes and 120 minutes. Thereafter, the amount of β-hexosaminidase released into the culture medium was measured by an enzyme-linked immunosorbent assay (ELISA) reader.

[0102] Table 3

[0103]

[0104]

[0105] The experimental results, as shown in Table 3 and Figure 3 It can be seen that the β-hexosaminidase release inhibitory effect of Preparation Example 1 containing the mixed extract of the present application is the most excellent compared to Preparation Examples 2 to 5.

[0106] Experimental Example 3. Collagen synthesis promoting effect - Collagen synthesis

[0107] The collagen production promoting effect of Preparation Examples 1 to 5 above was measured.

[0108] 1) Experimental method

[0109] After human dermal fibroblasts (Lonza, Swiss) were sub-cultured in DMEM containing 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin, they were inoculated into a 96-well cell culture plate and cultured for 24 hours at 37°C in a 5% carbon dioxide (CO2) atmosphere. Thereafter, after replacing the medium with serum-free medium, the diluted samples were treated with the cells and the supernatant was recovered after culturing for 24 hours, and the amount of collagen synthesis was measured by a procollagen type I C-peptide PIPEIA kit (Takara, Japan). 20 μL of the supernatant in each well was mixed with 100 μL of an antibody-POD conjugate solution dissolved at room temperature to react for 3 hours. Thereafter, after washing 3 times with 500 μL of phosphate-buffered saline, 100 μL of a color reagent was treated. After reacting for 15 minutes, the same amount of 1N sulfuric acid (Daejung, Korea) was added and reacted, and the absorbance was measured at 450 nm by a microplate reader (VersaMax TM , USA).

[0110] 2) Experimental results

[0111] The collagen production promotion rate was calculated by the following mathematical formula 1, and is shown in Table 4 below.

[0112] Mathematical formula 1

[0113]

[0114] Table 4

[0115]

[0116]

[0117] As shown in Table 4 and Figure 4 , it can be seen that the collagen synthesis promotion rate of the different treatment concentrations of Preparation Example 1 containing the mixed extract of the present application is the most excellent compared to Preparation Examples 2 to 5.

[0118] Experimental Example 4. Confirmation of skin effect

[0119] 1) Experimental methods

[0120] The cosmetic compositions prepared in Example 1 and Comparative Examples 1 to 4 were applied twice a day for four consecutive weeks to both sides and the front of the face of 30 test subjects (men and women aged 20-45 years). The improvement in skin hydration was measured before application, two weeks after application, and four weeks after application using a skin moisture meter (Corneometer CM 820, Courage+Khazaka, Germany). The unit was any unit (Arbitrary Unit, AU) provided by the instrument. The higher the measured value, the higher the moisture content of the skin surface.

[0121] 2) Experimental Results

[0122] The experimental results on skin hydration improvement are shown in Table 5.

[0123] Table 5

[0124]

[0125] The experimental results are shown in Table 5 and Figure 5 As shown, it can be seen that, compared with Comparative Examples 1 to 4, Example 1, which contains the mixed extract of the present invention, has the best skin moisturizing improvement effect.

[0126] Experimental Example 5. Confirmation of the rate of inhibition of transdermal water loss

[0127] 1) Experimental methods

[0128] The cosmetic compositions prepared in Example 1 and Comparative Examples 1 to 4 were used to designate the upper arm area of ​​30 test subjects (men and women aged 20-45 years) as the test site, and the area was marked with a horizontal and vertical dimension of 2×2cm. 2 Transdermal water loss was measured after applying the cosmetic composition to the test sites. The sites were thoroughly massaged and transdermal water loss was measured using a skin moisture loss tester (TEWAMETER TM210, C+K eletronic GmhH., Germany). The percentage increase in moisture content was measured at 30 minutes, 1 day, 4 days, and 8 days after application, using the initial moisture content at 0 hours as a baseline, to evaluate the short-term moisturizing effect. During measurement, the probe was held vertically in contact with the skin for 60 seconds, and the inhibition rate of transdermal water loss was measured three times consecutively. Measurements were conducted under identical conditions in a temperature and humidity controlled chamber; higher values ​​indicate less moisture loss.

[0129] Table 6

[0130]

[0131] The experimental results, as shown in Table 6 and Figure 6 Table 7, can know that the transdermal water loss inhibition rate of Example 1 containing the mixed extract of the present application is the best compared with Comparative Examples 1 to 4.

[0132] Dosage Form Example 1. Preparation of a toner dosage form

[0133] The prescription of the toner using the mixed extract of the present application is shown in Table 7 below.

[0134] Table 7

[0135]

[0136]

[0137] Dosage Form Example 2. Preparation of an emulsion dosage form

[0138] The prescription of the emulsion using the mixed extract of the present application is shown in Table 8 below.

[0139] Table 8

[0140]

[0141] Dosage Form Example 3. Preparation of a cream dosage form

[0142] The prescription of the cream using the mixed extract of the present application is shown in Table 9 below, for example.

[0143] Table 9

[0144]

[0145] Although the present application has been described with reference to the accompanying drawings, it is merely an example of various embodiments including the gist of the present application, and its purpose is to make it easy for those skilled in the art to implement, and it is obvious that the present application is not limited to the above-described embodiments. Therefore, the scope of protection of the present application should be interpreted according to the appended claims, and all technical ideas within the equivalent scope formed by alteration, replacement, substitution, etc. within the scope of the gist of the present application. Also, it is clear that, in order to more clearly explain the structure, a part of the structure in the drawings is exaggerated or reduced than the actual.

Claims

1. A cosmetic composition comprising a mixture of extracts of mistletoe, plantain, mallow, and okra as active ingredients, characterized in that, The mixture comprises a mixed extract obtained by mixing a mixture of mistletoe, plantain, mallow, and okra in a weight ratio of 0.1–2.5:0.1–2.5:0.1–2.5:0.1–2.5 with an extraction solvent in a weight ratio of 0.1–2.0:10.0–30.0, and then extracting at a temperature of 80–100°C for 2–6 hours, wherein the mixed extract is extracted with an extraction solvent selected from the group consisting of purified water and anhydrous or aqueous lower alcohols having a carbon number of 1 to 4.

2. The cosmetic composition according to claim 1, comprising a mixed extract of mistletoe, plantain, mallow, and okra as an active ingredient, is characterized in that, After cooling the above mixed extract to room temperature, it was filtered with a filter cloth and concentrated under reduced pressure.

3. A method for preparing a cosmetic composition comprising a mixed extract of mistletoe, plantain, mallow, and okra as an active ingredient, characterized in that, include: In the mixture preparation step (S10), mistletoe, plantain, mallow and okra are mixed in a weight ratio of 0.1-2.5:0.1-2.5:0.1-2.5:0.1-2.5 to prepare a mixture. In the extraction step (S20), the mixture prepared in the above-mentioned mixture preparation step (S10) is mixed with any one of the following extraction solvents selected from the group consisting of purified water and anhydrous or aqueous lower alcohols having 1 to 4 carbon atoms at a weight ratio of 0.1 to 2.0: 10.0 to 30.0, and then extracted at a temperature of 80 to 100°C for 2 to 6 hours to extract the mixed extract. as well as In the filtration and concentration step (S30), the mixed extract from the extraction step (S20) is cooled, filtered, and concentrated under reduced pressure.

4. The method for preparing a cosmetic composition comprising a mixed extract of mistletoe, plantain, mallow, and okra as an active ingredient, as described in claim 3, is characterized in that... In the above filtration and concentration step (S30), the mixed extract from the above extraction step (S20) is cooled at room temperature, then filtered with a 200-600 mesh filter cloth and concentrated under reduced pressure.

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