A Mongolian medicine for dispersing stasis and detoxifying, and its preparation method and application

Abstract

The invention discloses a Mongolian medicine for dispersing silt and detoxifying, and its raw materials include by mass: 8-12 parts of Terminalia chebula, 3-5 parts of Herba Discoloris, 6-10 parts of Aucklandiae Radix, 3-5 parts of Heiyunxiang, 5-7 parts of Acorus calamus, 3-5 parts of Sword Bean, 5-7 parts of Fructus Choerospondiae, 3-5 parts of Gypsum, 8-12 parts of concentrated buffalo horn powder, 3-5 parts of Bear Gall Powder, 1-3 parts of Calculus Bovis, 3-5 parts of Ophiopogonis, 3-5 parts of Radix Aconiti Lateralis, 3-5 parts of Radix Aconiti Lateralis, 3-5 parts of Crocus, 8-12 parts of Radix Aconiti Radix Aconiti Carmichaelii, 4-10 parts of Yeast β-glucan, 0.1-1 parts of β-glucanase, 1-5 parts of Konjac Flos, 5-10 parts of β-cyclodextrin, 1-2 parts of Disintegrant, 1-2 parts of Lubricant, and 1-2 parts of Flavor Corrective. The invention also discloses a preparation method of the Mongolian medicine for dispersing silt and detoxifying. The invention also discloses application of the Mongolian medicine for dispersing blood stasis and detoxifying in preparing medicine for treating sore throat.

Description

Technical Field

[0001] The invention relates to the technical field of Mongolian medicines, and in particular to a Mongolian medicine for dispersing blood stasis and detoxifying, and a preparation method and application thereof. Background Art

[0002] With the acceleration of modern life and seasonal changes, various bacteria, viruses and some physical and chemical factors can cause inflammation of the oral cavity and pharynx, such as acute and chronic pharyngitis, laryngitis, tonsillitis, oral inflammation and other common clinical infectious diseases. These diseases often cause patients to have dry and itchy pharynx, sore throat, and difficulty breathing, which have a certain impact on our lives and work.

[0003] For the treatment of pharyngitis, most of the clinical treatments are drug treatments, and Western medicine treatments mostly use anti-inflammatory drugs as the main treatment. At present, Western medicine treatment can only temporarily control the condition. Taking too many anti-inflammatory drugs, antibiotics or sulfonamides will cause the body's yin and yang to lose balance, the immune function is weakened, and the resistance is reduced, causing the condition to recur and be difficult to cure.

[0004] Traditional Chinese medicine can be used to clear away heat and detoxify, regulate qi and resolve phlegm. Traditional Chinese medicine shows greater advantages in treatment and has great potential for the complete treatment of this chronic disease. With the continuous improvement of living standards, people are paying more and more attention to the health care of their body functions. The pursuit of a good life, study and work status has become a necessity in life. Therefore, the development of safe, convenient and long-term use of throat clearing products for the prevention and treatment of oral and pharyngeal inflammation, removal of bad breath and relief of throat discomfort has broad market prospects.

[0005] Mongolian medicine believes that every disease is caused by the imbalance of the three roots of the human body due to external factors such as diet and daily life. The treatment aims to adjust the levels of the three roots of the human body to maintain a relative balance, and adopts a dialectical treatment method. Traditional Mongolian medicine is widely used in various clinical departments and has a unique effect. It has long-term experience and a long history. Mongolian medicine resources are rich, with no risk of drug resistance and drug residues, and low toxicity. The lozenges made of Mongolian medicine for pharyngitis have excellent market prospects. Summary of the invention

[0006] The purpose of the present invention is to solve the shortcomings of the prior art and to propose a Mongolian medicine for dispersing blood stasis and detoxifying, and a preparation method and application thereof.

[0007] A Mongolian medicine for dispersing blood stasis and detoxifying. The raw materials thereof include, by mass, 8-12 parts of terminalia chebula, 3-5 parts of herba discoloris, 6-10 parts of costusroot, 3-5 parts of osmanthus fragrans, 5-7 parts of calamus, 3-5 parts of sword bean, 5-7 parts of jujube, 3-5 parts of gypsum, 8-12 parts of concentrated buffalo horn powder, 3-5 parts of bear gall powder, 1-3 parts of bezoar, 3-5 parts of truncatum, 3-5 parts of rhizoma aconiti kusnezoffii, 3-5 parts of saffron, 8-12 parts of processed aconite, 4-10 parts of yeast beta-glucan, 0.1-1 parts of beta-glucanase, 1-5 parts of konjac flour, 5-10 parts of beta-cyclodextrin, 1-2 parts of disintegrant, 1-2 parts of lubricant and 1-2 parts of flavor corrective.

[0008] Preferably, the disintegrant is at least one of starch, dextrin, sucrose, lactose, mannitol and sorbitol.

[0009] Preferably, the flavoring agent is at least one of sucralose, aspartame, citric acid, menthol, and mint essence.

[0010] Preferably, the lubricant is at least one of magnesium stearate, calcium stearate and talc.

[0011] The preparation method of the Mongolian medicine for dispersing blood stasis and detoxifying comprises the following steps:

[0012] S1, add terminalia chebula, herba discolor, costusroot, radix atractylodes, calamus, sword bean, jujube, gypsum, buffalo horn concentrated powder, bear bile powder, bezoar, aconiti lobata, saffron and aconite prepared into water and boil, concentrate the extract at 50-60°C to obtain a concentrated solution; add medicinal ethanol to the concentrated solution until the ethanol mass fraction of the system reaches 60-70%, stir and let stand for 10-15h, filter to obtain a prefabricated material;

[0013] S2. Add yeast β-glucan to sodium hydrogen phosphate-citrate buffer, stir at 90-100° C. for 5-15 min, cool to 40-50° C., add β-glucanase for enzymolysis for 2-6 h, inactivate, centrifuge, add konjac flour, β-cyclodextrin, and prefabricated material to the supernatant, mix evenly, dry, add disintegrant, lubricant, and flavoring agent thereto, mix evenly, dry under reduced pressure, mix and granulate, and tablet to obtain Mongolian medicine for dispersing blood stasis and detoxification.

[0014] Preferably, in S1, the specific operation of decoction is as follows: add terminalia chebula, herbaceous scutellaria, costusroot, osmanthus fragrans, calamus tataricus, sword bean, jujube, gypsum, buffalo horn concentrated powder, bear bile powder, bezoar, aconite, saffron and processed aconite into water and decoct twice, each time for 1.5-2.5h, each time with a water to medicinal material mass ratio of 5.5-6.5:1, and then combine the extracts.

[0015] Preferably, in S1, the relative density of the concentrated liquid is 1.10-1.15.

[0016] Preferably, in S2, konjac flour, β-cyclodextrin and prefabricated materials are added to the supernatant, mixed evenly, and then dried at 50-60° C. to a water content of 4-7 wt %.

[0017] Preferably, in S2, the reduced pressure drying temperature is 40-50°C.

[0018] The application of the above-mentioned Mongolian medicine for dispersing blood stasis and detoxifying in the preparation of medicines for treating sore throat.

[0019] The technical effects of the present invention are as follows:

[0020] The yeast beta-glucan used in the present invention has excellent effects of enhancing immunity, anti-oxidation and healing wounds, and has significant biological activity. However, the strong hydrogen bonding between yeast beta-glucan molecules makes it insoluble in water. Therefore, the present invention uses beta-glucanase to enzymolyze the yeast beta-glucan, so as to promote the helical structure therein to unwind, and the surface contains a large number of active hydroxyl groups, which are then combined with beta-cyclodextrin to form a dynamic three-dimensional network structure in the system, which can not only make the drug stay in the pharyngeal lesion site for a long time, but also can continuously exert the drug effect, effectively avoid the first-pass effect of the liver, have high bioavailability, greatly enhance the stability of the tablet, and have good temperature and humidity resistance.

[0021] The applicant has found through experiments that when enzymatically hydrolyzed yeast β-glucan is not added, the product can reduce the content of pro-inflammatory cytokines and effectively inhibit the production of inflammatory mediators, but it has almost no effect on increasing anti-inflammatory cytokines, and the stability of the tablets is greatly reduced; while the addition of enzymatically hydrolyzed yeast β-glucan and the compounding with Mongolian medicine can not only greatly reduce the content of IL-1β, which is not the case without the addition of enzymatically hydrolyzed yeast β-glucan, but also effectively increase the content of IL-10. The present application can be achieved by simultaneously inhibiting the production of inflammatory cytokines and enhancing the production of anti-inflammatory cytokines.

[0022] Compared with existing drugs for treating pharyngitis, the present invention can achieve the goal of treating both the symptoms and the root causes, has a definite curative effect, can avoid the drawbacks of drug resistance, toxic side effects and dysbacteriosis caused by continuous use of antibiotics in Western medicine antibacterial and anti-inflammatory therapy, and has high safety. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 This is a comparison chart of the tensile strength of the Mongolian medicine for dispersing blood stasis and detoxifying obtained in Example 5 and Comparative Examples 1-2 after being stored at different temperatures.

[0024] Figure 2 This is a comparison chart of the tensile strength of the Mongolian medicine for dispersing blood stasis and detoxifying obtained in Example 5 and Comparative Examples 1-2 after being stored at different humidity levels.

[0025] Figure 3It is a comparison chart of IL-1α and IL-6 contents in the control group, model group, aspirin group, Example 5 group, Comparative Example 1 group and Comparative Example 2 group.

[0026] Figure 4 It is a comparison chart of IL-1β and IL-10 contents in the control group, model group, aspirin group, Example 5 group, Comparative Example 1 group and Comparative Example 2 group. DETAILED DESCRIPTION

[0027] The present invention will be further explained below in conjunction with specific embodiments. Example 1

[0028] A Mongolian medicine for dispersing blood stasis and detoxifying, the raw materials of which include: 8g of Terminalia chebula, 3g of Herba Discoloris, 6g of Aucklandia lappa, 3g of Rhizoma Cibotii, 5g of Acorus calamus, 3g of Sword bean, 5g of Ziziphus jujuba, 3g of Gypsum, 8g of concentrated powder of buffalo horn, 3g of bear bile powder, 1g of in vitro cultured bezoar, 3g of Rhizoma Cibotii, 3g of Rhizoma Cibotii, 3g of Saffron, 8g of processed Aconitum, 4g of yeast β-glucan, 0.1g of β-glucanase, 1g of konjac flour, 5g of β-cyclodextrin, 1g of starch, 1g of calcium stearate and 1g of citric acid.

[0029] The preparation method of the Mongolian medicine for dispersing blood stasis and detoxifying comprises the following steps:

[0030] S1. Add Terminalia chebula, Herba Pogostemonis Discolor, Radix Aucklandiae Radix, Rhizoma Cibotii, Rhizoma Acoruscae, Radix jackfruit, Gypsum, Concentrated powder of buffalo horn, bear bile powder, in vitro cultured bezoar, Rhizoma Cibotii, Rhizoma Aconiti Lateralis, Stigma Croci and Radix Aconiti Radix prepared into water and decoct for 2 times, each time for 1.5 hours, each time with a water to medicinal material mass ratio of 5.5:1, and then combine the extracts; concentrate the extracts at 50°C to obtain a concentrate with a relative density of 1.10; add 95% medicinal ethanol to the concentrate until the ethanol mass fraction of the system is 60%, stir and let stand for 10 hours, and filter to obtain a prefabricated material;

[0031] S2, adding yeast β-glucan to 30g of sodium hydrogen phosphate-citrate buffer with a pH value of 4.5-5, stirring at 90°C for 5min, cooling to 40°C, adding β-glucanase for enzymolysis for 2h, inactivating, centrifuging, adding konjac flour, β-cyclodextrin, and prefabricated material to the supernatant and mixing evenly, drying at 50°C to a water content of 4wt%, adding starch, calcium stearate, and citric acid thereto and mixing evenly, drying under reduced pressure at 40°C to constant weight, mixing and granulating to obtain Mongolian medicine for dispersing silt and detoxifying. Example 2

[0032] A Mongolian medicine for dispersing blood stasis and detoxifying, the raw materials of which include: 12g of Terminalia chebula, 5g of Herba Discoloris, 10g of Aucklandia lappa, 5g of Rhizoma Cibotii, 7g of Acorus calamus, 5g of Sword bean, 7g of Ziziphus jujuba, 5g of Gypsum, 12g of concentrated powder of buffalo horn, 5g of bear bile powder, 3g of in vitro cultured bezoar, 5g of Rhizoma Cibotii, 5g of Rhizoma Cibotii, 5g of Crocus sativus, 12g of processed Aconitum kusnezoffii, 10g of yeast β-glucan, 1g of β-glucanase, 5g of konjac flour, 10g of β-cyclodextrin, 2g of sucrose, 2g of magnesium stearate and 2g of menthol.

[0033] The preparation method of the Mongolian medicine for dispersing blood stasis and detoxifying comprises the following steps:

[0034] S1. Add terminalia chebula, herba discolor, costusroot, radix aucklandiae, calamus tataricus, sword bean, jujube, gypsum, buffalo horn concentrated powder, bear bile powder, in vitro cultured bezoar, aconiti lobata, saffron and processed aconite into water and decoct twice, each time for 2.5 hours, each time with a water to medicinal material mass ratio of 6.5:1, and then combine the extracts; concentrate the extracts at 60°C to obtain a concentrate with a relative density of 1.15; add 95% medicinal ethanol to the concentrate until the ethanol mass fraction of the system is 70%, stir and let stand for 15 hours, and filter to obtain a prefabricated material;

[0035] S2, yeast β-glucan is added to 50g sodium hydrogen phosphate-citrate buffer with a pH value of 4.5-5, stirred at 100°C for 15min, cooled to 50°C, β-glucanase is added for enzymolysis for 6h, inactivated, centrifuged, konjac flour, β-cyclodextrin and prefabricated material are added to the supernatant and mixed evenly, dried at 60°C to a water content of 7wt%, sucrose, magnesium stearate and menthol are added thereto and mixed evenly, dried under reduced pressure at 50°C to constant weight, mixed granulated and tableted to obtain Mongolian medicine for dispersing silt and detoxifying. Example 3

[0036] A Mongolian medicine for dispersing blood stasis and detoxifying, the raw materials of which include: 9g of Terminalia chebula, 4.5g of Herba Discoloris, 7g of Aucklandia lappa, 4.5g of Rhizoma Cibotii, 5.5g of Acorus calamus, 4.5g of Sword bean, 5.5g of Ziziphus jujuba, 4.5g of Gypsum, 9g of concentrated buffalo horn powder, 4.5g of bear bile powder, 1.5g of in vitro cultured bezoar, 4.5g of Rhizoma Cibotii, 3.5g of Rhizoma Cibotii, 4.5g of Crocus sativus, 9g of processed Aconitum kusnezoffii, 8g of yeast β-glucan, 0.3g of β-glucanase, 4g of konjac flour, 7g of β-cyclodextrin, 1.7g of mannitol, 1.2g of calcium stearate and 1.8g of aspartame.

[0037] The preparation method of the Mongolian medicine for dispersing blood stasis and detoxifying comprises the following steps:

[0038] S1. Add terminalia chebula, herba discolor, costusroot, radix aucklandiae, calamus tataricus, sword bean, jujube, gypsum, buffalo horn concentrated powder, bear bile powder, in vitro cultured bezoar, aconiti lobata, saffron and prepared aconite into water and decoct twice, each time for 1.8 hours, each time with a water to medicinal material mass ratio of 6.2:1, and then combine the extracts; concentrate the extracts at 53°C to obtain a concentrate with a relative density of 1.13; add 95% medicinal ethanol to the concentrate until the ethanol mass fraction of the system is 64%, stir and let stand for 14 hours, and filter to obtain a prefabricated material;

[0039] S2, yeast β-glucan is added to 35g sodium hydrogen phosphate-citrate buffer with a pH value of 4.5-5, stirred at 97°C for 8min, cooled to 46°C, β-glucanase is added for enzymolysis for 3h, inactivated, centrifuged, konjac flour, β-cyclodextrin and prefabricated material are added to the supernatant and mixed evenly, dried at 57°C to a water content of 5wt%, mannitol, calcium stearate and aspartame are added thereto and mixed evenly, dried under reduced pressure at 48°C to constant weight, mixed and granulated and tableted to obtain Mongolian medicine for dispersing silt and detoxifying. Example 4

[0040] A Mongolian medicine for dispersing blood stasis and detoxifying, the raw materials of which include: 11g of Terminalia chebula, 3.5g of Herba Discoloris, 9g of Aucklandiae Radix, 3.5g of Rhizoma Cibotii, 6.5g of Acorus calamus, 3.5g of Sword Bean, 6.5g of Ziziphus jujuba, 3.5g of Gypsum, 11g of concentrated buffalo horn powder, 3.5g of bear bile powder, 2.5g of in vitro cultured bezoar, 3.5g of Rhizoma Cibotii, 4.5g of Rhizoma Cibotii, 3.5g of Crocus sativus, 11g of processed Aconitum kusnezoffii, 6g of yeast β-glucan, 0.7g of β-glucanase, 2g of konjac flour, 9g of β-cyclodextrin, 1.3g of lactose, 1.8g of magnesium stearate and 1.2g of mint essence.

[0041] The preparation method of the Mongolian medicine for dispersing blood stasis and detoxifying comprises the following steps:

[0042] S1. Add terminalia chebula, herba discolor, costusroot, radix aucklandiae, calamus tataricus, sword bean, jujube, gypsum, buffalo horn concentrated powder, bear bile powder, in vitro cultured bezoar, aconiti lobata, saffron and prepared aconite into water and decoct twice, each time for 2.2 hours, each time with a water to medicinal material mass ratio of 5.8:1, and then combine the extracts; concentrate the extracts at 57°C to obtain a concentrate with a relative density of 1.11; add 95% medicinal ethanol to the concentrate until the ethanol mass fraction of the system is 66%, stir and let stand for 12 hours, and filter to obtain a prefabricated material;

[0043] S2. Add yeast β-glucan to 45g sodium dihydrogen phosphate-citric acid buffer with a pH value of 4.5-5, stir at 93°C for 12min, cool to 44°C, add β-glucanase for enzymolysis for 5h, inactivate, centrifuge, add konjac flour, β-cyclodextrin, and prefabricated material to the supernatant, mix evenly, dry at 53°C to a water content of 6wt%, add lactose, magnesium stearate, and mint essence thereto, mix evenly, dry under reduced pressure at 42°C to constant weight, mix and granulate to obtain Mongolian medicine for dispersing silt and detoxifying. Example 5

[0044] A Mongolian medicine for dispersing blood stasis and detoxifying, the raw materials of which include: 10g of Terminalia chebula, 4g of Herba Discoloris, 8g of Aucklandia lappa, 4g of Rhizoma Cibotii, 6g of Acorus calamus, 4g of Sword bean, 6g of Ziziphus jujuba, 4g of Gypsum, 10g of concentrated buffalo horn powder, 4g of bear bile powder, 2g of in vitro cultured bezoar, 4g of Rhizoma Cibotii, 4g of Rhizoma Cibotii, 4g of Crocus sativus, 10g of processed Aconitum kusnezoffii, 7g of yeast β-glucan, 0.5g of β-glucanase, 3g of konjac flour, 8g of β-cyclodextrin, 1.5g of dextrin, 1.5g of magnesium stearate and 1.5g of sucralose.

[0045] The preparation method of the Mongolian medicine for dispersing blood stasis and detoxifying comprises the following steps:

[0046] S1. Add Terminalia chebula, Herba Pogostemonis Discolor, Radix Aucklandiae Radix, Rhizoma Cibotii, Rhizoma Acoruscae, Radix jackfruit, Gypsum, Concentrated powder of buffalo horn, bear bile powder, in vitro cultured bezoar, Rhizoma Cibotii, Rhizoma Aconiti Kusnezoffii, Saffron and Radix Aconiti Kusnezoffii into water and decoct twice, each time for 2 hours, each time with a water to medicinal material mass ratio of 6:1, and then combine the extracts; concentrate the extracts at 55°C to obtain a concentrate with a relative density of 1.12; add 95% medicinal ethanol to the concentrate until the ethanol mass fraction of the system is 65%, stir and let stand for 13 hours, and filter to obtain a prefabricated material;

[0047] S2, adding yeast β-glucan to 40g sodium hydrogen phosphate-citrate buffer with a pH value of 4.5-5, stirring at 95°C for 10min, cooling to 45°C, adding β-glucanase for enzymolysis for 4h, inactivating, centrifuging, adding konjac flour, β-cyclodextrin, and prefabricated material to the supernatant, mixing evenly, drying at 55°C to a water content of 5.5wt%, adding dextrin, magnesium stearate, and sucralose thereto, mixing evenly, drying under reduced pressure at 45°C to constant weight, mixing and granulating to obtain Mongolian medicine for dispersing silt and detoxifying.

[0048] Comparative Example 1

[0049] A Mongolian medicine for dispersing blood stasis and detoxifying, the raw materials of which include: 10g of Terminalia chebula, 4g of Herba Discoloris, 8g of Aucklandiae Radix, 4g of Rhizoma Cibotii, 6g of Acorus calamus, 4g of Sword Bean, 6g of Ziziphus jujuba, 4g of Gypsum, 10g of concentrated buffalo horn powder, 4g of bear bile powder, 2g of in vitro cultured bezoar, 4g of Rhizoma Cibotii, 4g of Rhizoma Cibotii, 4g of Crocus sativus, 10g of processed Aconitum kusnezoffii, 7g of microcrystalline cellulose, 0.5g of beta-glucanase, 3g of konjac flour, 8g of beta-cyclodextrin, 1.5g of dextrin, 1.5g of magnesium stearate and 1.5g of sucralose.

[0050] The preparation method of the Mongolian medicine for dispersing blood stasis and detoxifying comprises the following steps:

[0051] S1. Add Terminalia chebula, Herba Pogostemonis Discolor, Radix Aucklandiae Radix, Rhizoma Cibotii, Rhizoma Acoruscae, Radix jackfruit, Gypsum, Concentrated powder of buffalo horn, bear bile powder, in vitro cultured bezoar, Rhizoma Cibotii, Rhizoma Aconiti Kusnezoffii, Saffron and Radix Aconiti Kusnezoffii into water and decoct twice, each time for 2 hours, each time with a water to medicinal material mass ratio of 6:1, and then combine the extracts; concentrate the extracts at 55°C to obtain a concentrate with a relative density of 1.12; add 95% medicinal ethanol to the concentrate until the ethanol mass fraction of the system is 65%, stir and let stand for 13 hours, and filter to obtain a prefabricated material;

[0052] S2, add microcrystalline cellulose to 40g sodium dihydrogen phosphate-citrate buffer with a pH value of 4.5-5, stir at 95°C for 10min, cool to 45°C, add β-glucanase for enzymolysis for 4h, inactivate, centrifuge, add konjac flour, β-cyclodextrin, and prefabricated material to the supernatant, mix evenly, dry at 55°C to a water content of 5.5wt%, add dextrin, magnesium stearate, and sucralose thereto, mix evenly, dry under reduced pressure at 45°C to constant weight, mix and granulate to obtain Mongolian medicine for dispersing silt and detoxifying.

[0053] Comparative Example 2

[0054] A Mongolian medicine for dispersing blood stasis and detoxifying, the raw materials of which include: 10g of Terminalia chebula, 4g of Herba Discoloris, 8g of Radix Aucklandiae, 4g of Rhizoma Cibotii, 6g of Acorus calamus, 4g of Sword Bean, 6g of Ziziphus jujuba, 4g of Gypsum, 10g of concentrated powder of buffalo horn, 4g of bear bile powder, 2g of in vitro cultured bezoar, 4g of Rhizoma Cibotii, 4g of Rhizoma Cibotii, 4g of Crocus sativus, 10g of processed Aconitum kusnezoffii, 3g of konjac flour, 8g of beta-cyclodextrin, 1.5g of dextrin, 1.5g of magnesium stearate and 1.5g of sucralose.

[0055] The preparation method of the Mongolian medicine for dispersing blood stasis and detoxifying comprises the following steps:

[0056] S1. Add Terminalia chebula, Herba Pogostemonis Discolor, Radix Aucklandiae Radix, Rhizoma Cibotii, Rhizoma Acoruscae, Radix jackfruit, Gypsum, Concentrated powder of buffalo horn, bear bile powder, in vitro cultured bezoar, Rhizoma Cibotii, Rhizoma Aconiti Kusnezoffii, Saffron and Radix Aconiti Kusnezoffii into water and decoct twice, each time for 2 hours, each time with a water to medicinal material mass ratio of 6:1, and then combine the extracts; concentrate the extracts at 55°C to obtain a concentrate with a relative density of 1.12; add 95% medicinal ethanol to the concentrate until the ethanol mass fraction of the system is 65%, stir and let stand for 13 hours, and filter to obtain a prefabricated material;

[0057] S2, konjac flour, β-cyclodextrin, and prefabricated material are added into water and mixed evenly, dried at 55° C. to a water content of 5.5wt%, dextrin, magnesium stearate, and sucralose are added thereto and mixed evenly, dried under reduced pressure at 45° C. to constant weight, mixed and granulated and tableted to obtain Mongolian medicine for dispersing silt and detoxifying.

[0058] Tensile strength test

[0059] The tablets to be tested were cut into strips of 15×20 mm and their tensile strength was measured using a TX-XT Plus texture analyzer. The test speed was 1 mm / s and three parallel measurements were performed. The tensile strength was calculated as follows:

[0060] Tensile strength = maximum tensile force / (sample width × sample thickness)

[0061] The unit of tensile strength is MPa, the unit of maximum tensile force is N, the unit of sample width is mm, and the unit of sample thickness is mm.

[0062] The Mongolian medicine for dispersing silt and detoxifying obtained in Example 5 and Comparative Examples 1-2 was stored at different temperatures (4°C, 25°C, 40°C) and a relative humidity of 13% for 4 days, and the tensile strength was measured. Figure 1 shown.

[0063] The Mongolian medicine for dispersing silt and detoxifying obtained in Example 5 and Comparative Examples 1-2 was stored at 25° C. and different relative humidity conditions (13%, 53%, 80%) for 4 days, and the tensile strength was measured. Figure 2 shown.

[0064] Depend on Figure 1 and Figure 2 It can be seen that the tensile strength of the Mongolian medicine for dispersing silt and detoxifying obtained in Example 5 is the highest under different storage conditions. The applicant believes that this is because the present invention uses β-glucanase to enzymolyze yeast β-glucan, which promotes the helical structure to unwind, and the surface contains a large number of active hydroxyl groups, which are then combined with β-cyclodextrin to form a dynamic three-dimensional network structure in the system, which greatly enhances the stability of the tablet and has good temperature and humidity resistance.

[0065] B / C mice (20±2g, half male and half female, SPF grade) were used as test subjects and were first fed with basic feed. Then they were randomly divided into 6 groups, namely control group, model group, aspirin group, Example 5 group, Comparative Example 1 group and Comparative Example 2 group. Among them, the aspirin group was gavaged with aspirin (0.27g / kg), the Example 5 group, Comparative Example 1 group and Comparative Example 2 group were gavaged with the suspension (1.17 g / kg) of the Mongolian medicine for dispersing stasis and detoxification obtained in Example 5 and Comparative Example 1-2, respectively, and the model group was gavaged with the same volume of deionized water. The drug was administered continuously for 7 days. Except for the control group, each group of mice was intraperitoneally injected with 0.67% glacial acetic acid solution 0.2mL per mouse 40min after administration to create a peritonitis model group.

[0066] Blood was collected from the orbital venous plexus 20 minutes after the injection of glacial acetic acid, and the serum was separated and the contents of IL-1α, IL-1β, IL-6, and IL-10 in the serum were determined using an enzyme marker. Figure 3 and Figure 4 shown.

[0067] Depend on Figure 3 and Figure 4 It can be seen that compared with the model group, the aspirin group, the Example 5 group, the Comparative Example 1 group and the Comparative Example 2 group can significantly reduce the content of pro-inflammatory cytokines (IL-1α and IL-6), but the aspirin group, the Comparative Example 1 group and the Comparative Example 2 group have significant differences in the effects on the content of IL-1β and IL-10. However, the Example 5 group can significantly reduce the content of pro-inflammatory cytokines (IL-1α, IL-1β and IL-6) and increase the content of anti-inflammatory cytokines (IL-10), confirming that the Mongolian medicine for dispersing stasis and detoxification obtained in this application can simultaneously inhibit the generation of inflammatory cytokines and enhance anti-inflammatory cytokines, thereby having an effective anti-inflammatory effect.

[0068] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A Mongolian medicine for dispersing blood stasis and detoxifying, characterized in that: The raw materials include, by mass: 8-12 parts of terminalia chebula, 3-5 parts of herba discoloris, 6-10 parts of costusroot, 3-5 parts of osmanthus sinensis, 5-7 parts of calamus, 3-5 parts of sword bean, 5-7 parts of jujube, 3-5 parts of gypsum, 8-12 parts of buffalo horn concentrated powder, 3-5 parts of bear bile powder, 1-3 parts of bezoar, 3-5 parts of truncatum, 3-5 parts of saffron, 3-5 parts of saffron, 8-12 parts of processed aconite, 4-10 parts of yeast β-glucan, 0.1-1 parts of β-glucanase, 1-5 parts of konjac flour, 5-10 parts of β-cyclodextrin, 1-2 parts of disintegrant, 1-2 parts of lubricant and 1-2 parts of flavoring agent; Prepared by the following steps: S1, add terminalia chebula, herba discolor, costusroot, radix atractylodes, calamus, sword bean, jujube, gypsum, buffalo horn concentrated powder, bear bile powder, bezoar, aconiti lobata, saffron and aconite prepared into water and boil, concentrate the extract at 50-60°C to obtain a concentrated solution; add medicinal ethanol to the concentrated solution until the ethanol mass fraction of the system reaches 60-70%, stir and let stand for 10-15h, filter to obtain a prefabricated material; S2. Add yeast β-glucan to sodium hydrogen phosphate-citrate buffer, stir at 90-100° C. for 5-15 min, cool to 40-50° C., add β-glucanase for enzymolysis for 2-6 h, inactivate, centrifuge, add konjac flour, β-cyclodextrin, and prefabricated material to the supernatant, mix evenly, dry, add disintegrant, lubricant, and flavoring agent thereto, mix evenly, dry under reduced pressure, mix and granulate, and tablet to obtain Mongolian medicine for dispersing blood stasis and detoxification.

2. The Mongolian medicine for dispersing stasis and detoxifying according to claim 1, characterized in that: The disintegrant is at least one of starch, dextrin, sucrose, lactose, mannitol and sorbitol.

3. The Mongolian medicine for dispersing stasis and detoxifying according to claim 1, characterized in that: The flavor corrector is at least one of sucralose, aspartame, citric acid, menthol, and mint essence.

4. The Mongolian medicine for dispersing stasis and detoxifying according to claim 1, characterized in that: The lubricant is at least one of magnesium stearate, calcium stearate and talc.

5. A method for preparing the Mongolian medicine for dispersing stasis and detoxifying as claimed in any one of claims 1 to 4, characterized in that: The steps include: S1, add terminalia chebula, herba discolor, costusroot, radix atractylodes, calamus, sword bean, jujube, gypsum, buffalo horn concentrated powder, bear bile powder, bezoar, aconiti lobata, saffron and aconite prepared into water and boil, concentrate the extract at 50-60°C to obtain a concentrated solution; add medicinal ethanol to the concentrated solution until the ethanol mass fraction of the system reaches 60-70%, stir and let stand for 10-15h, filter to obtain a prefabricated material; S2. Add yeast β-glucan to sodium hydrogen phosphate-citrate buffer, stir at 90-100° C. for 5-15 min, cool to 40-50° C., add β-glucanase for enzymolysis for 2-6 h, inactivate, centrifuge, add konjac flour, β-cyclodextrin, and prefabricated material to the supernatant, mix evenly, dry, add disintegrant, lubricant, and flavoring agent thereto, mix evenly, dry under reduced pressure, mix and granulate, and tablet to obtain Mongolian medicine for dispersing blood stasis and detoxification.

6. The method for preparing the Mongolian medicine for dispersing silt and detoxifying according to claim 5, characterized in that: In S1, the specific operation of decoction is as follows: add terminalia chebula, herbaceous scutellaria, costusroot, osmanthus fragrans, calamus tatarinowii, sword bean, jujube, gypsum, buffalo horn concentrate powder, bear bile powder, bezoar, aconiti lobata, saffron and processed aconite into water and decoct twice, each time for 1.5-2.5h, each time with a water to medicinal material mass ratio of 5.5-6.5:1, and then combine the extracts.

7. The method for preparing the Mongolian medicine for dispersing silt and detoxifying according to claim 5, characterized in that: In S1, the relative density of the concentrate is 1.10-1.

15.

8. The method for preparing the Mongolian medicine for dispersing silt and detoxifying according to claim 5, characterized in that: In S2, konjac flour, β-cyclodextrin and prefabricated materials are added to the supernatant, mixed evenly, and then dried at 50-60° C. to a water content of 4-7 wt %.

9. The method for preparing the Mongolian medicine for dispersing silt and detoxifying according to claim 5, characterized in that: In S2, the reduced pressure drying temperature is 40-50°C.

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