Biological experiment device and high and low temperature control method

Abstract

The application discloses a biological experiment device and a high-low temperature control method. The biological experiment device comprises a base, a temperature adjusting assembly, a first box assembly and a second box assembly which are sequentially stacked. The base is arranged at the bottom and has a first through hole and a second through hole which are communicated with the outside. The temperature adjusting assembly comprises a refrigeration part and a heating part which are communicated with each other. The first through hole, the heating part and the first box assembly are sequentially communicated. The second through hole, the refrigeration part and the second box assembly are sequentially communicated. The biological experiment device is provided with the base, the temperature adjusting assembly, the first box assembly and the second box assembly which are sequentially stacked. The refrigeration and the heating are integrated on the same device, the high-low temperature areas are separated, the energy utilization rate is improved, the overall operation complexity of the experiment is reduced, and the operation cost is reduced.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of biological experiment equipment, in particular to a biological experiment device and a high and low temperature control method. BACKGROUND

[0002] Biological experiment refers to a process of observing and studying the structure and life activity phenomenon of biological body which is not easy to observe under normal circumstances, by using certain instruments, materials and drugs, under specific environmental conditions, and through scientific methods. In biological experiments, in order to meet the experimental requirements of different temperatures, multiple equipment and instruments are usually arranged in a laboratory.

[0003] However, some of the multiple equipment and instruments need to absorb heat from the environment, and some need to release waste heat from the environment, resulting in low overall energy utilization. Especially in an environment with large temperature difference, the energy required by the equipment and instruments will be more, which will further lead to higher operating cost and greater energy waste. In addition, in the prior art, many equipment and instruments are scattered, and the same experiment needs to be transferred between different equipment and instruments, which not only increases the complexity of operation, but also increases the uncertainty risk of the experiment. SUMMARY

[0004] Therefore, the present application provides a biological experiment device and a high and low temperature control method to solve the problems of low overall energy utilization, complex overall operation of experiment and high operating cost caused by using multiple biological experiment equipment and instruments in the prior art.

[0005] The present application provides a biological experiment device and a high and low temperature control method, the biological experiment device comprises a base, a temperature adjusting assembly, a first box assembly and a second box assembly which are sequentially stacked, the base is arranged at the bottom and has a first through hole and a second through hole which are communicated with the outside, the temperature adjusting assembly comprises a refrigeration part and a heating part which are communicated with each other.

[0006] The first through hole, the heating part and the first box assembly are sequentially communicated, and the second through hole, the refrigeration part and the second box assembly are sequentially communicated.

[0007] Optionally, the biological experiment device comprises a ventilation connecting pipe, the ventilation connecting pipe is communicated with the refrigeration part and the second box assembly.

[0008] Optionally, the heating part comprises a first heat exchanger and a first exhaust pipe, the first heat exchanger is located in the heating part, and the first exhaust pipe is arranged outside the heating part and communicated with the outside of the biological experiment device.

[0009] A first collecting pipe is arranged between the refrigeration part and the heating part.

[0010] The refrigeration part, the first collecting pipe, the first heat exchanger and the first exhaust pipe are in communication with each other.

[0011] Optionally, the first air inlet and the first air outlet are arranged at the bottom and the top of the heating part respectively, the third through hole is arranged at the bottom of the first box assembly, and the first air inlet, the first air outlet and the third through hole are in communication with each other.

[0012] Optionally, the first recovery pipe is further arranged at the top of the heating part, the fourth through hole is arranged at the bottom of the first box assembly, and the first recovery pipe is communicated with the fourth through hole.

[0013] Optionally, the second box assembly comprises a frame member, the frame member is internally formed with a first cavity and a second cavity which are spaced from each other, the first cavity is communicated with the air connection pipe, and a filter part is arranged in the first cavity, and the second cavity is arranged with an automatic device for protein folding.

[0014] Optionally, the fifth through hole is arranged at the sidewall of the first cavity, the air connection pipe is connected to the fifth through hole, the second air outlet is arranged at the bottom of the first cavity, and the second air outlet is communicated with the second cavity.

[0015] Optionally, the filter part comprises a first filter member and a second filter member, the first filter member is connected to the fifth through hole, and the second filter member is arranged at the second air outlet.

[0016] Optionally, a plurality of threaded through holes are arranged at the bottom of the second cavity, and the threaded through holes are arranged with the automatic device.

[0017] Optionally, the third air outlet is arranged at the bottom of the periphery of the frame member, and the third air outlet is communicated with the air outlet of the threaded through hole.

[0018] The application further provides a high and low temperature control method of the biological experiment device, comprising:

[0019] The first natural wind entering the second through hole is delivered to the refrigeration part, the refrigeration part performs refrigeration treatment on the first natural wind to form first hot air and first cold air;

[0020] The first cold air enters the second box assembly, is filtered in the second box assembly, and forms first clean cold air for use in the second box assembly;

[0021] The second natural wind entering the first through hole is delivered to the heating part, and the heating part performs heating treatment on the second natural wind to form second hot air.

[0022] The second hot air enters the first box assembly, the first box assembly generates second cold air after use, and the second cold air enters the heating part for circulation;

[0023] The first hot air enters the heating part through the waste heat recovery pipe, the heating part is heat exchanged to form third cold air, and the third cold air is discharged out of the biological experiment device.

[0024] The beneficial effects of the present application are: different from the prior art, the present application sets up a biological experiment device including a base, a temperature adjusting assembly, a first box assembly and a second box assembly stacked in sequence, so that the first box assembly for high temperature and the second box assembly for low temperature are integrated on the same equipment, and the high and low temperature areas are separated, and experiments with two different temperature requirements can be carried out at the same time; secondly, the present application sets up a first through hole, a heating part and a first box assembly in sequence, so that the biological experiment device can realize the heating function, and provide heat for the first box assembly for high temperature; in addition, the present application sets up a second through hole, a refrigeration part and a second box assembly in sequence, so that the biological experiment device can realize the refrigeration function, and provide cold air for the first box assembly for low temperature; in addition, the present application sets up a refrigeration part and a heating part in communication with each other, so that the waste heat inside the biological experiment device can be utilized mutually, and the environment is not discharged with too much waste heat, which can improve the energy utilization rate, reduce environmental pollution, facilitate maintenance and management, and increase the flexibility of experiments.

[0025] It should be understood that the above general description and the following detailed description are only exemplary and explanatory, but not limiting the present application. BRIEF DESCRIPTION OF DRAWINGS

[0026] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings needed in the embodiment description will be briefly introduced. Obviously, the drawings in the following description are only some embodiments of the present application, and other drawings can be obtained by those skilled in the art without creative labor.

[0027] Figure 1 is a structural schematic view of the biological experiment device of the present application;

[0028] Figure 2 is a structural schematic view of the front of the temperature adjusting assembly of the present application;

[0029] Figure 3 is a structural schematic view of the back of the temperature adjusting assembly of the present application;

[0030] Figure 4 is a structural schematic view of the second box assembly of the present application;

[0031] Figure 5is a structural schematic diagram of the front of the biological experiment device of the application;

[0032] Figure 6 is a structural schematic diagram of the back of the biological experiment device of the application;

[0033] Figure 7 is a refrigeration part principle diagram of the biological experiment device of the application;

[0034] Figure 8 is a heating part principle diagram of the biological experiment device of the application;

[0035] Figure 9 is a running principle diagram of the biological experiment device of the application;

[0036] Figure 10 is a flow chart of the high and low temperature control method of the biological experiment device of the application.

[0037] In the figure, the reference signs are as follows: 100, base; 110, first through hole; 120, second through hole; 200, temperature adjusting assembly; 240, frame; 210, refrigeration part; 211, second air inlet; 212, fourth air outlet; 213, cold air connecting pipe; 214, first compressor; 215, first condenser; 216, first liquid storage bottle; 217, first expansion valve; 218, first evaporator; 219, first fan; 241, second fan; 220, heating part; 221, first exhaust pipe; 222, first air inlet; 223, first air supply pipe; 224, first recovery pipe; 225, second compressor; 226, second condenser; 227, second liquid storage bottle; 228, second expansion valve; 229, second evaporator; 251, third fan; 252, fourth fan; 253, second dryer; 254, second filter; 255, first heat exchanger; 256, second heat exchanger; 257, temperature and humidity sensor; 230, first collection pipe; 300, first box assembly; 310, third through hole; 320, fourth through hole; 400, second box assembly; 410, frame piece; 411, threaded through hole; 412, third air outlet; 413, multi-layer heat insulation glass; 414, box door; 420, first cavity; 421, fifth through hole; 422, second air outlet; 423, top plate; 430, filtering part; 431, first filtering piece; 432, second filtering piece; 440, second cavity; 500, air connection pipe. DETAILED DESCRIPTION

[0038] In order for those skilled in the art to better understand the technical solutions of the present application, the biological experiment device and the high and low temperature control method provided by the present application are further described in detail below in combination with the drawings and specific embodiments. It can be understood that the described embodiments are only some of the embodiments of the present application, not all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative labor fall within the scope of protection of the present application.

[0039] The terms "first", "second", and the like in the present application are used to distinguish different objects, not to describe a specific order. In addition, the terms "include" and "have" and any variations thereof are intended to cover non-exclusive inclusion. For example, a process, method, system, product or device that includes a series of steps or units is not limited to the listed steps or units, but can optionally include steps or units not listed, or can optionally include other steps or units inherent to the process, method, product or device.

[0040] Biomedical experiments need to control high temperature, normal temperature and low temperature environment throughout the experiment, in order to be applied in different experimental steps, some experimental steps need to be carried out in low temperature environment, some experimental steps need to be carried out in high temperature environment, in order to meet the experimental requirements.

[0041] For example, PCR reaction: PCR reaction needs to be carried out multiple times to separate DNA strands and catalyze reaction, and at the same time, DNA annealing and enzyme reaction buffer need to be carried out at low temperature.

[0042] DNA / RNA electrophoresis: DNA / RNA electrophoresis needs to store and process samples at low temperature to prevent RNA / DNA molecular degradation; in the process of electrophoretic separation, high temperature electrophoretic buffer needs to be used to increase the migration speed of DNA / RNA molecules.

[0043] Protein separation and purification: in the process of purification, the stability of protein needs to be maintained at low temperature, and at the same time, high temperature needs to be used in some steps to catalyze reaction or remove impurities.

[0044] Cell growth: cell growth experiment needs to culture cells at constant temperature. For example, cells need to be grown in a constant temperature incubator at 37℃. But there are also cells that need to be treated at low temperature, such as cryopreservation or low temperature stress experiment.

[0045] Therefore, in biomedical laboratories, incubators are usually set between 4℃ and 65℃ to accommodate the preservation needs of different kinds of biological products and chemical reagents. It is necessary to equip devices that can operate normally under low-temperature and high-temperature environments, such as PCR instruments, low-temperature refrigerators, high-temperature incubators, etc. In addition, various biological experiments such as cell culture and cell survival rate determination also require the use of biological experimental devices with multiple environmental temperatures, such as incubators, thermostats, drying ovens, etc., to control the experimental environment and ensure the accuracy and repeatability of experimental results.

[0046] The present application provides a biological experimental device and a high and low temperature control method, taking cell culture requiring high temperature and protein folding requiring low temperature as examples, which are not limited to cell culture and protein folding, and describes the biological experimental device and the high and low temperature control method to solve the problems of low overall energy utilization, complex overall operation, and high running cost caused by the use of multiple biological experimental equipment and instruments.

[0047] Please refer to Figures 1 to 10 , Figure 1 is a structural schematic diagram of the biological experimental device of the present application; Figure 2 is a structural schematic diagram of the front of the temperature adjusting assembly of the present application; Figure 3 is a structural schematic diagram of the back of the temperature adjusting assembly of the present application; Figure 4 is a structural schematic diagram of the second box assembly of the present application; Figure 5 is a structural schematic diagram of the front of the biological experimental device of the present application; Figure 6 is a structural schematic diagram of the back of the biological experimental device of the present application; Figure 7 is a principle diagram of the refrigeration part of the biological experimental device of the present application; Figure 8 is a principle diagram of the heating part of the biological experimental device of the present application; Figure 9 is a running principle diagram of the biological experimental device of the present application; Figure 10 is a flowchart of the high and low temperature control method of the biological experimental device of the present application.

[0048] In an embodiment, as Figures 1 to 6As shown, the biological experiment device can be used for cell culture and protein folding. The biological experiment device can include a base 100, a temperature regulating assembly 200, a first box assembly 300, and a second box assembly 400 arranged in sequence. The base 100 can be arranged at the bottom and have a first through hole 110 and a second through hole 120 that can be connected to the outside. The first through hole 110 and the second through hole 120 can respectively introduce the first natural wind and the second natural wind to the heating part 220 and the cooling part 210. The temperature regulating assembly 200 can include the cooling part 210 and the heating part 220 that are connected to each other. The first hot air generated by the cooling part 210 can be transmitted to the heating part 220 for recycling. The first through hole 110, the heating part 220, and the first box assembly 300 can be sequentially connected to form a heating channel. The second through hole 120, the cooling part 210, and the second box assembly 400 can be sequentially connected to form a cooling channel.

[0049] In the embodiments of the present application, by arranging the base 100, the temperature regulating assembly 200, the first box assembly 300, and the second box assembly 400 in sequence, the base 100 is arranged at the bottom to form the biological experiment device. The first box assembly 300 is used for high-temperature cell culture, and the second box assembly 400 is used for low-temperature protein folding. The biological experiment device has high and low temperature functions and can perform two different temperature experiments on the same device. Secondly, the cooling part 210 is connected to the heating part 220, so that the waste heat generated by the cooling part 210 can be transmitted to the heating part 220 for heating, increasing the recycling rate of energy and promoting the green environment. In addition, the first through hole 110, the heating part 220, and the first box assembly 300 are sequentially connected, and the second through hole 120, the cooling part 210, and the second box assembly 400 are sequentially connected. The biological experiment device can realize cooling and heating functions, separate high and low temperature areas, and perform two different temperature experiments at the same time. The first box assembly 300 and the second box assembly 400 operate independently relative to the thermostat, and the temperature between them can be accurately controlled. The thermostat can realize multiple temperature fields and can be used for different temperature experiments to ensure the accuracy and repeatability of the experimental results.

[0050] The cooling part 210 can set the control temperature range to 4-25℃, and the suitable temperature for protein folding can be selected according to the need. According to the cell culture experiment, the heating part 220 can set the control temperature range to 25-65℃, and the most suitable cell culture experiment temperature can be selected according to the type of cells and bacteria and the experimental requirements.

[0051] In some embodiments, as Figure 2 and Figure 3As shown, the heating part 220 can include a first heat exchanger 255, which can be located inside the heating part 220, and a first exhaust pipe 221, which can be arranged outside the heating part 220 and can be connected to the outside of the biological experiment device. The first collection pipe 230 can be arranged between the cooling part 210 and the heating part 220, and the cooling part 210, the first collection pipe 230, the first heat exchanger 255, and the first exhaust pipe 221 can be in communication with each other. Specifically, the first collection pipe 230 can be arranged as a waste heat collection pipe. The waste heat discharged from the cooling part 210 is not directly discharged to the outside of the device, but is transported to the heating part 220 through the waste heat collection pipe for waste heat utilization. After passing through the first heat exchanger 255, the waste heat is converted into low-temperature air and is discharged from the first exhaust pipe 221.

[0052] Optionally, a plurality of grooves are arranged on the side of the base 100 facing the bottom, which facilitates the natural wind to enter the first through hole 110 and the second through hole 120. The base 100 can be a vibration isolation base 100, which is a multi-axis vibration isolation base 100, which can reduce the vibration influence of the outside on the experiment platform to meet the vibration isolation requirements of precise biological experiments, and can also reduce the internal vibration when the temperature adjusting assembly 200 is working.

[0053] Optionally, the first exhaust pipe 221 can extend to the second through hole 120. The low-temperature air discharged from the first exhaust pipe 221 can enter the cooling part 210 again through the second through hole 120 along with the natural wind, thereby reducing the energy consumption of the cooling part 210 and saving energy.

[0054] In some embodiments, as shown in the drawings, Figure 1 As shown, the biological experiment device can include a ventilation connection pipe 500, which can be connected to the cooling part 210 and the second box assembly 400. The low-temperature cold air generated by the cooling part 210 can be transmitted to the second box assembly 400 through the ventilation connection pipe 500. The ventilation connection pipe 500 can be provided with a sterilization device to sterilize the low-temperature cold air entering the second box assembly 400, which is beneficial to protein folding in protein expression biological experiments.

[0055] The cooling part 210 delivers cold air to the second box assembly 400. The waste heat discharged by the cooling part 210 is collected and utilized by the heating part 220. After heat exchange treatment, the waste heat is cooled to low-temperature air and is discharged to the outside environment, thereby reducing the thermal pollution to the environment. Based on the principle of air energy heat pump, the heating part 220 collects the waste heat energy discharged by the cooling part 210 to reduce energy consumption, and uses electric energy to accurately control the temperature of the output hot air. The heating part 220 uses air circulation to ensure that the temperature of the first box assembly 300 is constant, that is, to deliver hot air to the first box assembly 300 and recover low-temperature air from the first box assembly 300 for heating.

[0056] Optionally, the bottom of the refrigeration part 210 is provided with a second air inlet 211, and the side of the refrigeration part 210 away from the heating part 220 is provided with a fourth air outlet 212. A cold air connecting pipe 213 is arranged between the refrigeration part 210 and the fourth air outlet 212. Natural wind enters the second air inlet 211 from the second through hole 120, and then enters the refrigeration part 210 to generate cold air which is discharged from the cold air connecting pipe 213 and the fourth air outlet 212 in turn, enters the air connection pipe 500, and enters the second box body assembly 400.

[0057] Optionally, the temperature adjusting assembly 200 further comprises a frame 240, the frame 240 is arranged around the refrigeration part 210 and the heating part 220, and the refrigeration part 210 and the heating part 220 are fixed on the frame 240. The temperature adjusting assembly 200 is connected with the first box body assembly 300 and the base 100, and the frame 240 can be used to protect the refrigeration part 210 and the heating part 220. The second air inlet 211 of the refrigeration part 210 is arranged at the bottom, and ambient wind is sucked in from the second air inlet 211. The cold wind output by the refrigeration part 210 is delivered to the fourth air outlet 212 through the cold air connecting pipe 213, and is delivered to the air connection pipe 500 through the fourth air outlet 212.

[0058] In some embodiments, the working principle of the refrigeration part 210 is as follows Figure 7As shown, the refrigeration part 210 further comprises a first compressor 214, a first condenser 215, a first liquid storage bottle 216, a first expansion valve 217, a first evaporator 218, a first fan 219 and a second fan 241. In the refrigeration process, the first compressor 214 absorbs low-temperature and low-pressure gaseous refrigerant from the first evaporator 218 to increase the temperature and pressure of the refrigerant, and convert the refrigerant into high-temperature and high-pressure gas. Then the refrigerant flows into the first condenser 215 to release heat, and the temperature is higher than the ambient temperature, so the heat needs to be dissipated. The second fan 241 operates to absorb low-temperature ambient air from the second air inlet 211 to cool the first condenser 215, and the ambient air after heat exchange becomes high-temperature waste heat air and is discharged to the first collection pipe 230. On the other hand, the high-temperature and high-pressure gaseous refrigerant is cooled by the first condenser 215 and becomes high-temperature and high-pressure liquid, and then the refrigerant flows into the first liquid storage bottle 216 to remove water and filter impurities, and the first liquid storage bottle 216 can also maintain the stability of the refrigerant flow. The high-temperature and high-pressure liquid refrigerant flowing out of the first liquid storage bottle 216 is injected through the first expansion valve 217, and at this time the refrigerant is rapidly cooled and decompressed, and is expanded into low-temperature and low-pressure mist. Then the low-temperature and low-pressure mist refrigerant enters the first evaporator 218, and the pressure is further reduced due to the sudden increase of the space of the evaporator, and the temperature and pressure of the refrigerant are further reduced. The first fan 219 operates to absorb ambient air and heat exchanges with the first evaporator 218, and since the temperature of the first evaporator 218 is much lower than the ambient temperature, it can quickly cool the ambient air to cold air, which is then blown and transmitted to the fourth air outlet 212 by the first fan 219. The low-temperature and low-pressure mist refrigerant is changed into low-temperature and low-pressure gas after heat exchange with the ambient air in the first evaporator 218, and continuously circulates through the driving of the compressor to achieve continuous refrigeration.

[0059] In some embodiments, the bottom and top of the heating part 220 can be respectively provided with a first air inlet 222 and a first air supply pipe 223, and the bottom of the first box assembly 300 can be provided with a third through hole 310. The first air inlet 222 can be communicated with the first through hole 110, and the first through hole 110, the first air inlet 222, the first air supply pipe 223 and the third through hole 310 can be communicated with each other. Natural wind enters from the first through hole 110, enters the heating part 220 through the first air inlet 222, and is transmitted out through the first air supply pipe 223 after the heating in the heating part 220 is completed, and enters the first box assembly 300 through the third through hole 310 for high-temperature cell culture.

[0060] In some embodiments, the heating part 220 can be further provided with a first recovery pipe 224 at the top thereof, and the first box assembly 300 can be provided with a fourth through hole 320 at the bottom thereof. When the inside of the first box assembly 300 is surrounded by hot air, the cold air inside the first box assembly 300 can be discharged through the fourth through hole 320, and the first recovery pipe 224 can be connected to the fourth through hole 320. The first recovery pipe 224 recovers the hot air discharged from the first box assembly 300 and re-enters the heating part 220 for recycling.

[0061] Optionally, the first air supply pipe 223 can extend to the top end inside the first box assembly 300 through the third through hole 310, so that the first air supply pipe 223 gradually penetrates the hot air from top to bottom, making the flow of gas inside the first box assembly 300 more gentle and the temperature more uniform, which is helpful for cell culture.

[0062] Optionally, the first recovery pipe 224 can be a short pipe that can be connected to the fourth through hole 320. When the upper part of the first box assembly 300 is filled with hot air and only a little cold air is left at the bottom, the first recovery pipe 224 can also discharge the cold air, thereby increasing the uniformity of the gas temperature inside the first box assembly 300.

[0063] The first air inlet 222 of the heating part 220 is used to inhale ambient air. The hot air generated by the heating part 220 is delivered to the first box assembly 300 by the first air supply pipe 223, and the low-temperature backflow air in the box is recovered from the air outlet of the first box assembly 300 by the first recovery pipe 224. During the heating process, the heating part 220 absorbs waste heat by performing a heat exchange treatment on the waste heat air inhaled from the first collection pipe 230. After the heat exchange treatment, the waste heat air is cooled to low-temperature air and then discharged to the external environment through the first exhaust pipe 221.

[0064] In some embodiments, the working principle of the heating part 220 is as shown in Figure 8 The refrigeration part 210 further includes a second compressor 225, a second condenser 226, a second liquid storage bottle 227, a second expansion valve 228, a second evaporator 229, a third fan 251, a fourth fan 252, a second dryer 253, and a second filter 254 connected in sequence. The second condenser 226 is in the second heat exchanger 256. The second filter 254 and the second dryer 253 can ensure a clean and dry environment for the first box assembly 300.

[0065] In the heating process, the second compressor 225 absorbs the low-temperature and low-pressure gaseous heating agent from the second evaporator 229 to increase the temperature and pressure of the heating agent, so as to convert the heating agent into high-temperature and high-pressure gas. Subsequently, the heating agent flows into the second condenser 226 to release heat, at which time the temperature is higher than the ambient temperature, and the heat can be used to heat the outside. The fourth fan 252 operates to absorb the low-temperature air flowing back to the first cabinet assembly 300 from the first recovery pipe 224, and the air first passes through a second dryer 253 to perform dehumidification, and then passes through the second heat exchanger 256 in which the second condenser 226 is located to increase the temperature, and then passes through a second filter 254 to filter impurities, and finally is discharged to the first air supply pipe 223 in the form of clean hot air. On the other hand, the high-temperature and high-pressure gaseous heating agent is cooled by the second condenser 226 to become high-temperature and high-pressure liquid, and then flows into the second liquid storage bottle 227 to remove water and filter impurities, and the second liquid storage bottle 227 can also maintain the stability of the flow of the heating agent. The high-temperature and high-pressure liquid heating agent flowing out of the second liquid storage bottle 227 is sprayed by the second expansion valve 228, at which time the heating agent is rapidly cooled and depressurized, and is expanded and vaporized to become low-temperature and low-pressure mist. Subsequently, the low-temperature and low-pressure mist of the heating agent enters the second evaporator 229, and the pressure is reduced due to the sudden increase in the space of the evaporator, and the pressure and temperature of the heating agent are further reduced. At this time, the low-temperature heating agent is a load for the heating part 220, and the lower the temperature, the higher the energy consumption of the heating part 220 required to consume, so it is necessary to find ways to increase the temperature of the heating agent flowing out of the second evaporator 229. In order to increase the temperature of the heating agent flowing out of the second evaporator 229, the third fan 251 operates to absorb the high-temperature air recovered from the waste heat from the first collection pipe 230, and the high-temperature air increases the temperature of the heating agent in the first heat exchanger 255, and at the same time, the high-temperature air is cooled to low-temperature air and discharged to the first exhaust pipe 221. Through waste heat recovery, the energy consumption requirement of the heating part 220 is reduced, and the waste heat pollution discharged to the outside environment is also reduced.

[0066] In some embodiments, the working principle of the temperature adjustment assembly 200 is as shown in FIG. 2B. Figure 9As shown, in operation, the refrigeration part 210 inhales ambient air from the first air inlet 222, and sends the cold air from the fourth air outlet 212 to the fifth through-hole 421 of the first cabinet assembly 300. Before entering the experimental area of the second cabinet assembly 400, the cold air is filtered and stabilized by the first filter, the first static pressure cavity 420 and the second filter 254, and then is discharged to the outside environment through the third air outlet 412. The waste heat air discharged by the refrigeration part 210 in the working process is transported to the first collection pipe 230 of the heating part 220 through the waste heat recovery pipe for waste heat utilization. The waste heat air after the first heat exchanger 255 is cooled to low-temperature air and is discharged to the outside environment through the first exhaust pipe 221 of the heating part 220. The heating part 220 also needs to inhale ambient air from the first air inlet 222 in the working process, and then send dry and clean hot air to the third through-hole 310 of the second cabinet assembly 400, and the low-temperature air discharged by the second cabinet assembly 400 is returned to the first recovery pipe 224 through the fourth through-hole 320.

[0067] Optionally, in order to realize the integrated precision temperature and humidity control of the biological experiment device, temperature and humidity sensors 257 can be installed at the first air inlet 222 of the refrigeration part 210, the first exhaust pipe 221 of the heating part 220, the experimental area in the second cabinet assembly 400, and the first cabinet assembly 300, respectively.

[0068] In some embodiments, as shown in FIG. 1, Figure 4 As shown, the second cabinet assembly 400 can include a frame member 410, and the frame member 410 can form a first cavity 420 and a second cavity 440 spaced from each other inside. The first cavity 420 can be communicated with the air connection pipe 500, and the first cavity 420 can be provided with a filter part 430. The second cavity 440 can place an automated device for protein folding. The cold air enters the first cavity 420 through the air connection pipe 500, is filtered and purified in the first cavity 420, and then enters the second cavity 440, which is suitable for protein folding experiments on the automated device in the second cavity 440. The first cavity 420 is a static pressure tank of the filtering system, which not only has the function of filtering and purifying the cold air to ensure the cleanliness of the incoming air, but also can stabilize the airflow and reduce the noise, so that the airflow entering the first cavity 420 is smoother and softer into the second cavity 440.

[0069] The first cavity 420 can be provided with a top plate 423 at the top, and the top plate 423 is used to close the first cavity 420, so that the first cavity 420 is a static pressure tank of the filtering system.

[0070] In some embodiments, the first cavity 420 can be provided with a fifth through hole 421, the ventilation connecting pipe 500 can be connected to the fifth through hole 421, the first cavity 420 can be provided with a second air outlet 422, the second air outlet 422 can be communicated with the second cavity 440, wherein the ventilation connecting pipe 500, the fifth through hole 421, the first cavity 420 and the second air outlet 422 form an air flow channel to enable cold air to enter the second cavity 440.

[0071] In some embodiments, the filter part 430 can include a first filter 431 and a second filter 432, the first filter 431 can be connected to the fifth through hole 421, the first filter 431 can be provided as a high-efficiency filter to preliminarily filter the cold air flow entering from the fifth through hole 421, the second filter 432 can be provided at the second air outlet 422 to filter the air flow in the first cavity 420, the second filter 432 can be provided as a filtering diffusion net to perform secondary filtering on the air flow and make the air flow entering the second cavity 440 more smooth, the first filter 431, the second filter 432 and the first cavity 420 form a low-temperature air filtering system.

[0072] Optionally, the second air outlet 422 can be provided as a plurality of second air outlets, and the second filter 432 can also be provided as a plurality of second filters, the plurality of second filters 432 can make the secondary filtering of the air flow more efficient. Specifically, the second air outlet 422 and the second filter 432 can each be provided as four second air outlets and four second filters to improve the smoothness of the secondary filtered air flow.

[0073] In some embodiments, the bottom of the second cavity 440 can be provided with a plurality of threaded through holes 411, the plurality of threaded through holes 411 can not only discharge the waste air flow in the second cavity 440 out of the device, but also be used to place automatic equipment.

[0074] Optionally, the bottom of the second cavity 440 can also be provided as a honeycomb plate to enable ventilation and installation of automatic instrument equipment.

[0075] In some embodiments, the bottom of the outer periphery of the frame 410 can be provided with a baffle, the baffle can be provided with a third air outlet 412, the third air outlet 412 can be communicated with the air outlet of the threaded through hole 411, when the frame 410 is square, the baffle at the bottom of the periphery of the frame 410 can be provided with a long-strip-shaped third air outlet 412, the third air outlet 412 can be provided as a long strip, and the third air outlet 412 can be used to discharge the air flow in the second cavity 440 out of the device.

[0076] Specifically, the cold wind in the ventilation connecting pipe 500 first needs to pass through the filtering of the high-efficiency filter before entering the first cavity 420 from the fifth through hole 421, and then the air flow is accumulated in the first cavity 420, and then the wind in the first cavity 420 passes through the filtering and scattering net at the second air outlet 422 for filtering and rectifying again, and then the air-conditioning wind after twice filtering and flow stabilization enters the experimental area in the second cavity 440, and finally passes through the threaded holes on the honeycomb plate and is discharged through the third air outlet 412.

[0077] The frame member 410 can be made of metal, and the periphery of the frame member 410 can be provided with transparent multilayer heat insulation glass 413. The multilayer heat insulation glass 413 can be provided in four pieces, and the four pieces of multilayer heat insulation glass 413 surround and enclose the frame member 410, so that the periphery of the second cavity 440 is enclosed. The multilayer heat insulation glass 413 can not only keep warm the space in the second cavity 440, but also facilitate observation of the internal experiment by the experimenters.

[0078] Optionally, the side of the frame member 410 opposite to the fifth through hole 421 is provided with a box door 414 that can be opened and closed. The box door 414 facilitates the placement of automatic experimental equipment and experimental apparatus in the second cavity 440, so as to better perform the protein folding experiment.

[0079] Optionally, a partition plate (not shown in the figure) can be arranged between the first box assembly 300 and the second box assembly 400. The partition plate can be filled with heat insulation cotton, and can isolate the temperature of the first box assembly 300 and the second box assembly 400, so as to avoid mutual influence between the first box assembly 300 and the second box assembly 400.

[0080] Optionally, the first box assembly 300 and the second box assembly 400 can be divided into multiple experimental sub-zones, so that each experimental sub-zone can independently perform an experiment. The first box assembly 300 and the second box assembly 400 can simultaneously perform multiple cell culture and protein folding, and can simultaneously perform multiple biological experiments.

[0081] Optionally, the biological experiment device can further be provided with an automatic transmission device. The automatic transmission device can be arranged between the first box assembly 300 and the second box assembly 400. The automatic transmission device can automatically move the experimental products in the first box assembly 300 and the second box assembly 400 when the experiment reaches a set time, and automatically perform the next step of the experiment, thereby avoiding manual movement and pollution to the experimental products.

[0082] It can be understood that the second box assembly 400 has sufficient space and mounting interface to provide a low temperature or room temperature experimental environment (for example, 16°-25°, the temperature range can be set according to requirements), and the second box assembly 400 has a larger cavity space, which can conveniently configure various protein folding automation instrument equipment and other biological experiment automation experimental instrument, and is equipped with transparent heat insulation glass, so that the experimenter can observe the experimental situation and perform necessary manual operation. In addition, by installing different automation equipment, each biological experiment device can form a biological experiment island, and multiple biological experiment islands for different experimental purposes can meet the cluster use of modern biological laboratories.

[0083] It can be understood that the second box assembly 400 and the first box assembly 300 of the biological experiment device are relatively independent, and at the same time, low temperature or room temperature experiments and high temperature experiments can be performed. In addition, the second box assembly 400 has a large space and is provided with a honeycomb plate for conveniently mounting various automation instruments, so that different instruments can be installed inside according to requirements to perform automatic experimental operation. In addition, the waste heat discharged by the biological experiment device during refrigeration can be recycled for heating, which not only reduces the energy consumption of the heating part 220, but also reduces the waste heat pollution discharged by the platform, thereby reducing the air conditioning load of the laboratory environment. The internal space of the biological experiment device forms an independent controllable constant temperature, clean and vibration isolation condition, and by arranging multiple biological experiment devices in a laboratory space, multiple differentiated and high environmental standard biological experiments can be performed in a common laboratory space.

[0084] As shown in Figure 10 The application also provides a high and low temperature control method of the biological experiment device, which comprises the following steps:

[0085] Step S100: The first natural wind entering the second through hole 120 is delivered to the refrigeration part 210, and the refrigeration part 210 performs refrigeration treatment on the first natural wind to form first hot air and first cold air;

[0086] Step S200: The first cold air enters the second box assembly 400 and forms first clean cold air after filtration in the second box assembly 400, which is used in the second box assembly 400;

[0087] Step S300: The second natural wind entering the first through hole 110 is delivered to the heating part 220, and the heating part 220 performs heating treatment on the second natural wind to form second hot air;

[0088] Step S400: The second hot air enters the first box assembly 300, and the first box assembly 300 generates second cold air after use, and the second cold air enters the heating part 220 for recycling;

[0089] Step S500: The first hot air enters the heating part 220 through the waste heat recovery pipe, and the third cold air is formed after the heating part 220 is heat exchanged and treated, and the third cold air is discharged outside the biological experimental device.

[0090] Specifically, the first natural wind entering the second through hole 120 is delivered to the refrigeration part 210, and the refrigeration part 210 performs refrigeration treatment on the first natural wind to form the first hot air and the first cold air. The first cold air enters the second box assembly 400, and the first clean cold air is formed after being filtered in the second box assembly 400 for use inside the second box assembly 400. The first hot air enters the heating part 220 through the waste heat recovery pipe, and the third cold air is formed after the heating part 220 is heat exchanged and treated, and the third cold air is discharged outside the biological experimental device. The waste heat generated by the refrigeration part 210 is treated to increase the utilization rate of energy. That is, when the refrigeration part 210 delivers cold air to the second box assembly 400, the waste heat discharged by the refrigeration part 210 is collected and utilized by the heating part 220. After heat exchange, the waste heat is cooled to low-temperature air and then discharged to the external environment, reducing the thermal pollution to the environment. The second natural wind entering the first through hole 110 is delivered to the heating part 220, and the heating part 220 performs heating treatment on the second natural wind to form the second hot air. The second hot air enters the first box assembly 300, and the second cold air is generated after being used by the first box assembly 300, and the second cold air enters the heating part 220 for recycling. That is, based on the principle of air energy heat pump, the heating part 220 reduces energy consumption by collecting the waste heat energy discharged by the refrigeration part 210, and precisely controls the temperature of the output hot air by using electric energy. On the other hand, the temperature of the first box assembly 300 is kept constant by using the air circulation method, and the hot air is delivered to the first box assembly 300, and the low-temperature air is recovered from the first box assembly 300 for heating.

[0091] The method designs an energy-saving system. The waste heat discharged by the refrigeration part 210 of the second box assembly 400 is collected and utilized for heating the heating part 220 for use in the first box assembly 300, which reduces the waste heat discharge of the refrigeration part 210 to the experimental environment and reduces the energy consumption of the heating part 220, achieving efficient use of energy. The second box assembly 400 and the first box assembly 300 are integrated, and the temperatures of the two boxes are independently controlled, which can meet the requirements of experiments under multiple temperature conditions at the same time, and the structure is compact. In addition, the second box assembly 400 has sufficient space and installation interfaces, and various automatic experimental instruments can be conveniently installed. By installing different automatic equipment in the second box assembly 400, each biological experimental device forms a biological experimental island, and multiple biological experimental islands with different functions can meet the cluster use of modern biological laboratories. Since each biological experimental island hardly discharges waste heat to the environment, even if multiple experimental equipment are arranged in the same experimental space, they will not affect each other and will not affect the air conditioning load of the external environment.

[0092] The application sets the biological experiment device to include the base 100, the temperature adjusting assembly 200, the first box assembly 300 and the second box assembly 400 which are stacked in sequence. The base 100 is arranged at the bottom of the biological experiment device, and the first through hole 110 and the second through hole 120 which are communicated with the outside are arranged at the bottom. The temperature adjusting assembly 200 is arranged to include the refrigeration part 210 and the heating part 220 which are communicated with each other, so that the heat generated by the refrigeration part 210 can be transmitted to the heating part 220 for recycling, increasing the energy utilization rate. The first through hole 110, the heating part 220 and the first box assembly 300 are sequentially communicated, so that the natural wind generates hot wind through the heating part 220 and enters the first box assembly 300 for use. The second through hole 120, the refrigeration part 210 and the second box assembly 400 are sequentially communicated, so that the natural wind generates cold wind through the refrigeration part 210 and enters the second box assembly 400 for use. The biological experiment device has the refrigeration and heating functions. The heating part 220 can also recycle the heat generated by the refrigeration part 210, improving the overall energy utilization rate. The same equipment can be used for high-temperature cell culture and low-temperature protein folding, reducing the complexity of the overall operation and the running cost. Secondly, the heating part 220 includes the first heat exchanger 255 and the first exhaust pipe 221. The first collection pipe 230 is arranged between the refrigeration part 210 and the heating part 220. The refrigeration part 210, the first collection pipe 230, the first heat exchanger 255 and the first exhaust pipe 221 can be communicated with each other. The waste heat of the refrigeration part 210 is transported to the heating part 220 through the waste heat collection pipe for waste heat utilization. After passing through the first heat exchanger 255, the waste heat is converted into low-temperature wind and is transmitted out from the first exhaust pipe 221. In addition, the first filter 431 is used for preliminarily filtering the cold air flow entering from the fifth through hole 421, and the second filter 432 is used for filtering the air flow in the first cavity 420, so that the air flow can be filtered twice and the air flow entering the second cavity 440 is smoother.

[0093] It should be noted that the various optional embodiments introduced in the embodiments of the application can be combined with each other to be implemented, or can be implemented independently, and the embodiments of the application are not limited thereto.

[0094] In the description of the application, it needs to be understood that the terms "upper", "lower", "left", "right", etc. indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the application and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a particular orientation, and a particular orientation configuration and operation. Therefore, it cannot be understood as a limitation on the application. In addition, "first", "second" are only for the purpose of description, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features limited by "first", "second" can explicitly or implicitly include one or more features. In the description of the application, unless otherwise specified, the meaning of "multiple" is two or more.

[0095] In the description of the application, it needs to be understood that unless otherwise explicitly specified and limited, the terms "mounting", "connecting", "connecting" and the like should be understood in a broad sense, for example, it can be fixedly connected, or it can be detachably connected, or integrally connected; it can be mechanically connected, or it can be electrically connected; it can be directly connected, or it can be indirectly connected through an intermediate medium, or it can be the communication inside two elements. For those skilled in the art, the specific meaning of the above terms in the application can be understood according to the specific circumstances.

[0096] The above embodiments are described with reference to the accompanying drawings, and other different forms and embodiments are also possible without departing from the principles of the application, so the application should not be construed as being limited to the embodiments presented herein. Rather, these embodiments are provided to make the application perfect and complete, and to convey the scope of the application to those skilled in the art. In the drawings, the sizes and relative sizes of components may be exaggerated for clarity. The terms used herein are only based on the purpose of describing specific embodiments, and are not intended to be limiting. The terms "comprise" and / or "include" are used in the specification to indicate the presence of the described features, integers, components and / or components, but do not exclude the presence or addition of one or more other features, integers, components, components and / or groups thereof. Unless otherwise indicated, when stated, the numerical range includes the upper and lower limits of the range and any sub-range therebetween.

[0097] The above only describes some embodiments of the application, and does not limit the protection scope of the application, and any equivalent device or equivalent process transformation using the contents of the application specification and drawings, or directly or indirectly used in other related technical fields, are also included in the patent protection scope of the application.

Claims

1. A biological experiment apparatus characterized by comprising: The biological experiment device comprises a base, a temperature adjusting assembly, a first box assembly and a second box assembly which are stacked in sequence; The base is arranged at the bottom and has a first through hole and a second through hole which are communicated with the outside, and the temperature adjusting assembly comprises a refrigeration part and a heating part which are communicated with each other; The first through hole, the heating part and the first box assembly are communicated in sequence, and the second through hole, the refrigeration part and the second box assembly are communicated in sequence; The heating part comprises a first heat exchanger and a first exhaust pipe, the first heat exchanger is arranged in the heating part, and the first exhaust pipe is arranged outside the heating part and is communicated with the outside of the biological experiment device; A first collecting pipe is arranged between the refrigeration part and the heating part; The first collecting pipe is arranged as a waste heat collecting pipe; The refrigeration part, the first collecting pipe, the first heat exchanger and the first exhaust pipe are communicated with each other; The first exhaust pipe extends to the second through hole, and the low-temperature air discharged from the first exhaust pipe enters the refrigeration part again through the second through hole along the natural wind; A first recycling pipe is further arranged at the top of the heating part, a fourth through hole is arranged at the bottom of the first box assembly, and the first recycling pipe is communicated with the fourth through hole; The biological experiment device comprises a ventilation connecting pipe which is communicated with the refrigeration part and the second box assembly; A first air inlet and a first air supply pipe are arranged at the bottom and the top of the heating part respectively, a third through hole is arranged at the bottom of the first box assembly, and the first air inlet, the first air supply pipe and the third through hole are communicated with each other; The first air supply pipe extends to the top end inside the first box assembly through the third through hole; The second box assembly comprises a frame, and a first cavity and a second cavity which are spaced apart are formed in the frame, the first cavity is communicated with the ventilation connecting pipe, a filter part is arranged in the first cavity, and the second cavity is used for placing an automatic device for protein folding; A top plate is further arranged at the top of the first cavity, and the top plate is used for closing the first cavity so that the first cavity is a static pressure box of a filtering system.

2. The biological experiment apparatus according to claim 1, wherein A fifth through hole is arranged on the side wall of the first cavity, the ventilation connecting pipe is connected to the fifth through hole, a second air outlet is arranged at the bottom of the first cavity, and the second air outlet is communicated with the second cavity.

3. The biological experiment apparatus according to claim 2, wherein The filter part comprises a first filter part and a second filter part, the first filter part is connected to the fifth through hole, and the second filter part is arranged at the second air outlet.

4. The biological experiment apparatus according to claim 2, wherein A plurality of threaded through holes are arranged at the bottom of the second cavity, and the threaded through holes are used for placing the automatic device.

5. A high and low temperature control method of a biological experiment device, characterized by, The method for the biological experiment device as claimed in any one of claims 1-4 comprises: The first natural air entering the second through hole is delivered to the refrigeration part, the refrigeration part performs refrigeration treatment on the first natural air to form first hot air and first cold air; The first cold air enters the second box assembly, is filtered in the second box assembly, and forms first clean cold air which is used in the second box assembly; The first natural wind entering the first through hole is transported to a heating part, the heating part performs heating treatment on the second natural wind to form second hot air; The second hot air enters a first box assembly, the first box assembly generates second cold air after use, and the second cold air enters the heating part for recycling; The first hot air enters the heating part through a waste heat recovery pipe, the heating part performs heat exchange treatment to form third cold air, and the third cold air is discharged out of the biological experimental device.

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