A whitening composition and a skin care product

Abstract

The application discloses a whitening composition and a skin care product, the whitening composition is prepared by mixing 4-butyl resorcinol, glabridin and glycyrrhizol according to a molar ratio of (3-5):1:1, and the skin care product contains the whitening composition; the three active components of the whitening composition synergistically act on different pathways to inhibit the activity of tyrosinase and reduce the content of melanin, multiple whitening synergies are realized, the glabridin and the glycyrrhizol can reduce the irritability of the 4-butyl resorcinol, and can be used as raw materials of the whitening skin care product.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of skin care products, and particularly relates to a whitening composition and a skin care product. BACKGROUND

[0002] In modern society, people's life rhythm is fast, and life pressure is big, plus environmental impact, skin often appears various problems, which needs to rely on skin care products to repair the skin. Whitening has been the main function of consumers' demand for skin care products, and white and clean skin is more and more praised by most women, and the concept of white covering a hundred ugliness makes consumers demand higher for whitening products.

[0003] The existing whitening skin care product adds single whitening active ingredient, such as only adding 4-butyl resorcinol or glabridin, 4-butyl resorcinol has certain irritation to the skin when used, and the whitening effect of single whitening component is poor. SUMMARY

[0004] The purpose of the present application is to solve the problems in the background art, and to provide a whitening composition and a skin care product.

[0005] To achieve the above purpose, the present application provides a whitening composition, which is mixed by 4-butyl resorcinol, glabridin and licorice chalcone A in a molar ratio of (3-5):1:1.

[0006] As a preferred, the whitening composition is mixed by 4-butyl resorcinol, glabridin and licorice chalcone A in a molar ratio of 5:1:1.

[0007] As a preferred, the whitening composition is mixed by 50uM 4-butyl resorcinol, 10uM glabridin and 10uM licorice chalcone A.

[0008] The present application also provides a skin care product containing the above-mentioned whitening composition, which includes but is not limited to essence, cream.

[0009] The efficacy of each component of the whitening composition: 4-butyl resorcinol can inhibit the production of melanin, and inhibit the activity of tyrosinase and TRP-1, inhibit the production of melanin by directly inhibiting the activity of tyrosinase and B16 melanoma cells, and does not cause any cytotoxicity; glabridin has a strong tyrosinase inhibitory effect, reduces the production of melanin by reducing the secretion of MSH (melanocyte stimulating hormone), in addition, its biological activities include antioxidant, anti-inflammatory, neuroprotective, anti-atherosclerotic, energy metabolism regulating effects, and also include anti-tumor, anti-nephritis and other effects; glychalon A is a flavonoid compound extracted from glabrous licorice, which has antibacterial, anti-inflammatory, anti-allergic and anti-photoaging pharmacological effects, and can inhibit the activity of tyrosinase, reduce the production of melanin by reducing the level of PGE2 (inflammatory factor).

[0010] The schematic diagram of part of the efficacy mechanism of the whitening composition is described in the reference literature Christina Wasmeier, Alistair N Hume, Giulia Bolasco, Miguel C Seabra. Melanosomes at a glance. J Cell Sci. 2008 Dec 15; 121 (Pt 24): 3995-9 in the art.

[0011] Advantages of the present application: the present application mixes 4-butyl resorcinol, glabridin and glychalon A in a specific ratio, the three active ingredients synergistically inhibit the activity of tyrosinase and reduce the content of melanin from different pathways, realize multiple whitening and synergistic effect, glabridin and glychalon A can reduce the irritation of 4-butyl resorcinol, and can be used as raw materials for whitening skin care products.

[0012] The features and advantages of the present application will be described in detail in conjunction with the embodiments and the accompanying drawings. BRIEF DESCRIPTION OF DRAWINGS

[0013] Figure 1 is the efficacy mechanism schematic diagram of the whitening composition of the embodiments of the present application. DETAILED DESCRIPTION

[0014] In order to make the purpose, technical scheme and advantages of the embodiments of the present application more clear, the technical scheme of the embodiments of the present application will be described clearly and completely in conjunction with the drawings in the embodiments of the present application.

[0015] Example 1

[0016] Reference Figure 1The present embodiment provides a whitening composition, which is formed by mixing 4-butylresorcinol, glabridin and licochalcone A at a molar ratio of 5:1:1, preferably, the whitening composition is formed by mixing 50 μM 4-butylresorcinol, 10 μM glabridin and 10 μM licochalcone A.

[0017] The present embodiment also provides a skin care product containing the above whitening composition, which is a whitening serum.

[0018] Example 2

[0019] Referring to Figure 1 The present embodiment provides a whitening composition, which is formed by mixing 4-butylresorcinol, glabridin and licochalcone A at a molar ratio of 4:1:1, preferably, the whitening composition is formed by mixing 40 μM 4-butylresorcinol, 10 μM glabridin and 10 μM licochalcone A.

[0020] The present embodiment also provides a skin care product containing the above whitening composition, which is a whitening serum.

[0021] Example 3

[0022] Referring to Figure 1 The present embodiment provides a whitening composition, which is formed by mixing 4-butylresorcinol, glabridin and licochalcone A at a molar ratio of 3:1:1, preferably, the whitening composition is formed by mixing 30 μM 4-butylresorcinol, 10 μM glabridin and 10 μM licochalcone A.

[0023] The present embodiment also provides a skin care product containing the above whitening composition, which is a whitening serum.

[0024] Comparative Example 1

[0025] The present embodiment provides a whitening composition, which is formed by mixing 4-butylresorcinol, glabridin and licochalcone A at a molar ratio of 2:1:1, preferably, the whitening composition is formed by mixing 20 μM 4-butylresorcinol, 10 μM glabridin and 10 μM licochalcone A.

[0026] Comparative Example 2

[0027] The present embodiment provides a whitening composition, which is formed by mixing 4-butylresorcinol, glabridin and licochalcone A at a molar ratio of 1:1:1, preferably, the whitening composition is formed by mixing 10 μM 4-butylresorcinol, 10 μM glabridin and 10 μM licochalcone A.

[0028] Comparative Example 3

[0029] The present embodiment provides a whitening composition, which is mixed by 4-butylresorcinol, glabridin and licochalcone A in a molar mass ratio of 10:1:1, preferably, the whitening composition is mixed by 100 μM 4-butylresorcinol, 10 μM glabridin and 10 μM licochalcone A.

[0030] I. Test on whitening efficacy of different whitening drugs

[0031] The MNT1 cells were cultured in the DMEM medium added with 20% serum and double antibodies, and placed in the culture dish of the cell culture box (37℃, 5% CO2 environment). The MNT1 cells were divided into 11 groups and placed in the same environment for culture. When the cell density reached 60%, the same amount of different whitening ingredients were added to each group (the whitening ingredients and concentration ratio added to each group are shown in Table 1 below). After 48 hours of culture, the cells in each group were trypsinized, centrifuged at 800 rpm for 5 minutes, resuspended by adding 200 μL of 20 mM Tris-HCl (pH 7.0) 0.1% Triton-X100, and lysed on ice for 30 min. Each lysate was divided into two parts. The first part was added with an equal volume of 1 mg / mL L-DOPA, and incubated at 37℃ for 4 h. The absorbance value was detected at 490 nm wavelength to detect the tyrosinase activity. The second part was added with RIPA lysis buffer, and centrifuged at 12000 rpm for 10 min at 4℃. The supernatant was used for BCA experiment for quantification, and the total protein concentration was detected. The precipitate was resuspended with 1M NaOH, and reacted at 50℃ for 1 h. The absorbance value was detected at 490 nm wavelength to detect the melanin content. During the experiment, the tyrosinase activity and the melanin content were corrected by the protein concentration. The cells in the blank control group were not added with whitening ingredients or treated with deionized water. The whitening ingredients were dissolved in DMSO and then added. Each whitening ingredient in Table 1 was purchased from the existing market. The specific test results of the tyrosinase activity and the melanin content of the cells in each group are shown in Table 1 below.

[0032] Table 1 Test table of tyrosinase activity and melanin content of MNT1 cells treated with different whitening ingredients

[0033]

[0034]

[0035] As shown in Table 1, the test results of tyrosinase activity of each group of cells are as follows: compared with the blank control group, the addition of 377, glabridin, licochalcone A and 4-butylresorcinol can significantly reduce the activity of tyrosinase. The test results of reducing tyrosinase activity are as follows: 377> licochalcone A> glabridin> 4-butylresorcinol, under the same concentration conditions, 4-butylresorcinol is not good for inhibiting the activity of tyrosinase. When 10 μM 4-butylresorcinol is combined with 10 μM licochalcone A, compared with the blank control group, the activity of tyrosinase can be significantly reduced, but compared with 377, the inhibitory effect is still not good; on this basis, 10 uM glabridin is added, and it is found that the inhibitory effect is not much different. When the concentration of 4-butylresorcinol reaches 50 μM, combined with 10 uM glabridin and 10 uM licochalcone A, the inhibitory efficiency is significantly increased, and is better than all the control groups. The whitening composition compounded according to the present application has an inhibitory efficiency of tyrosinase which is about 1.5 times higher than that of 4-butylresorcinol with the same concentration.

[0036] The above results show that when 4-butylresorcinol, glabridin and licochalcone A are used in combination, they can better inhibit the activity of tyrosinase than single whitening ingredient or double whitening ingredient, and when they are combined in the concentration ratio of 50 μM 4-butylresorcinol+10 μM glabridin+10 μM licochalcone A, the effect of inhibiting the activity of tyrosinase is the most significant. As shown in Table 1, the test results of melanin content of each group of cells are as follows: compared with the blank control, the addition of 377, glabridin, licochalcone A and 4-butylresorcinol can significantly reduce the melanin content of MNT1 cells. The test results of reducing melanin content are as follows: glabridin> 377> licochalcone A> 4-butylresorcinol, 4-butylresorcinol is not good for reducing the melanin content of MNT1 cells. When 10 μM 4-butylresorcinol is combined with 10 μM licochalcone A, compared with the blank control group, the melanin content of MNT 1 cells can be significantly reduced, but compared with 377, the effect is still not good; on this basis, 10 uM glabridin is added, and it is found that the effect of reducing the melanin content of MNT 1 cells is slightly improved. The melanin content of MNT 1 cells shows a downward trend with the increase of the concentration of 4-butylresorcinol in the composition (i.e. 4-butylresorcinol+10 μM glabridin+10 μM licochalcone). The whitening composition compounded according to the present application has an inhibitory efficiency of melanin production which is about 1.24 times higher than that of 4-butylresorcinol with the same concentration.

[0037] The above results show that when the three whitening ingredients 4-butylresorcinol, glabridin and licochalcone A are used in combination, the effect of reducing the MNT1 melanocytes is better than that of single whitening ingredient or double whitening ingredients, and when the concentration of 4-butylresorcinol reaches 50 μM, the effect of reducing melanin content is more obvious, which is better than all the control groups. It can be seen that when 50 μM 4-butylresorcinol + 10 μM glabridin + 10 μM licochalcone A are used in combination, the effect of reducing melanin content is the most significant.

[0038] From the above experiment, it can be known that the different proportions of the three ingredients 4-butylresorcinol, glabridin and licochalcone A in the whitening composition have a certain influence on the inhibition effect of tyrosinase activity and melanin content of cells, and when the whitening composition is compounded, too high or too low proportion of 4-butylresorcinol makes the effect of inhibiting the activity of tyrosinase and reducing the melanin content when the whitening composition is used cannot reach the best. While the whitening composition of the present application mixes 4-butylresorcinol, glabridin and licochalcone A according to a specific proportion, the three whitening active ingredients synergistically inhibit the activity of tyrosinase from different pathways, maximally reduce the tyrosinase activity and melanin content of cells, realize multiple whitening and synergistic effect, glabridin and licochalcone A can reduce the irritability of 4-butylresorcinol, so that the whitening composition is more mild when used, and can be used as raw material of whitening skincare products, and the concentration ratio of the three whitening active ingredients.

[0039] II. Whitening efficacy test of the whitening composition

[0040] The melanin (MNT1) cells were cultured in DMEM medium with 20% serum and double antibodies, and placed in a culture dish in a cell culture box (37°C, 5% CO2 environment). The MNT1 cells were divided into 5 groups and placed in the same environment for culture. When the cell density reached 60%, different whitening components were added to each group in the same amount. The whitening components and concentrations added to each group are shown in Table 2 below. After 48 hours of culture, the cells in each group were trypsinized and centrifuged at 800 rpm for 5 minutes. 200 μL of 20 mM Tris-HCl (pH 7.0) 0.1% Triton-X100 was added for resuspension, and the cells were lysed on ice for 30 min. The lysate in each group was divided into two parts. The first part was added with an equal volume of 1 mg / mL L-DOPA, and incubated at 37°C for 4 h. The absorbance value was detected at 490 nm wavelength, and the tyrosinase activity was detected. The second part was added with RIPA lysis buffer, and centrifuged at 12000 rpm for 10 min at 4°C. The supernatant was used for BCA experiment for quantification, and the total protein concentration was detected. The precipitate was resuspended with 1M NaOH, and reacted at 50°C for 1 h. The absorbance value was measured at 490 nm wavelength, and the melanin content was detected. During the experiment, the tyrosinase activity and the melanin content were corrected with the protein concentration. The cells in the blank control group were not treated with whitening components or treated with deionized water. The whitening components were dissolved in DMSO and then added. The whitening components in Table 2 were purchased from the existing market. The specific test results of the tyrosinase activity and the melanin content of the cells in each group are shown in Table 2 below.

[0041] Table 2 Test table of tyrosinase activity and melanin content of MNT1 cells treated with different whitening components

[0042]

[0043]

[0044] As shown in Table 2, the whitening composition composed of 100 μM 4-butylresorcinol + 10 μM glabriden + 10 μM glabrone has no obvious synergistic effect on inhibiting the tyrosinase activity and the generation of melanin compared with single whitening components such as 100 μM 4-butylresorcinol or 10 μM glabriden or glabrone.

[0045] The above examples are illustrative of the present application and are not limiting thereof. Any simple transformation of the present application also belongs to the protection scope of the present application.

Claims

1. A whitening composition characterized in that: The whitening composition is mixed by 4-butylresorcinol, glabridin and licochalcone A in a molar ratio of 5:1:

1.

2. The whitening composition of claim 1, wherein: The whitening composition is mixed by 50 μM 4-butylresorcinol, 10 μM glabridin and 10 μM licochalcone A.

3. A skin care product containing the whitening composition of claim 1 or 2.

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