A pesticide composition and use thereof

Abstract

The present application relates to synergistic compositions containing a biologically effective amount of compound I and compound II, the compound II is one of abamectin, emamectin benzoate, pymetrozine, chlorfenapyr, methoxyfenozide, tebufenozide, and their methods for controlling pests in agronomic and non-agronomic environments. The compound I has synergistic effect with compound II, which can improve the effect of pest control, reduce the amount of application, reduce the use frequency, and reduce the use cost.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of pesticide combinations, and specifically relates to a combination containing a biologically effective amount of compound I and at least one Glutamate-gated chloride channel (GluCl) allosteric modulator (such as abamectin, emamectin benzoate, etc.), an uncoupler that interferes with the proton gradient affecting oxidative phosphorylation (such as acequinocyl, etc.), a selective homopteran insect feeding blocker (such as pymetrozine, etc.), a ecdysone receptor agonist (such as methoxyfenozide, tebufenozide, etc.) insecticide, and methods thereof for controlling pests in agronomic and non-agronomic environments. BACKGROUND

[0002] In recent years, the occurrence of various crop pests has shown a trend of increasing year by year, and the occurrence area has increased year by year, which has brought great harm to crops, resulting in problems such as crop yield reduction and agricultural production cost increase. In order to improve the control effect on pests, farmers increase the amount of pesticide or randomly mix pesticides. This unscientific use of pesticides not only fails to achieve the synergistic effect, but also leads to problems such as waste of pesticides, excessive pesticide residues, pollution of the environment, and development of pesticide resistance in pests. Therefore, it is urgent to develop a synergistic composition for pests, which can achieve the effects of expanding the range of control objects, reducing the amount of pesticide, and delaying the development of resistance.

[0003] In the actual process of agricultural production, long-term continuous use of the same pesticide will quickly lead to pesticide resistance in pests, resulting in reduced control effect and increased pesticide use, which exacerbates pesticide residues in agricultural products and damage to the ecological environment. Compounding with insecticides of completely different mechanisms is an effective way to delay the development of pesticide resistance in pests, expand the insecticidal spectrum, prolong the service life, and reduce the amount of pesticides used. However, how to compound the pesticides to produce synergistic rather than antagonistic effects between the pesticides is a major difficulty.

[0004] Patent document CN 111909143 A discloses compound I, which has broad-spectrum insecticidal activity and good control effect on pests such as Lepidoptera, Hemiptera, Coleoptera, Thysanoptera, Diptera, and mites. However, it does not disclose how to compound it with other pesticides to achieve the purpose of synergism.

[0005] The structural formula of compound I is as follows,

[0006] SUMMARY

[0007] In order to improve the deficiencies of the prior art, the present application provides a composition comprising compound I and compound II, wherein the compound II is at least one of abamectin, emamectin benzoate, pymetrozine, chlorfenapyr, methoxyfenozide, tebufenozide;

[0008] The compound I has the following structural formula,

[0009]

[0010] According to an embodiment of the present application, the compound I is prepared by the method of Example 1 in the patent document CN 111909143 A, which is incorporated herein by reference in its entirety.

[0011] According to an embodiment of the present application, the mass ratio of the compound I and the compound II is 80-1:1-80.

[0012] According to an embodiment of the present application, the mass ratio of the compound I and the compound II is 50-1:1-50.

[0013] According to a preferred embodiment of the present application, the mass ratio of the compound I and the compound II is 10-1:1-10.

[0014] For example, the mass ratio of the compound I and the compound II is 50:1, 40:1, 30:1, 20:1, 10:1, 5:1, 4:1, 3:1, 2:1, 1:1, 1:2, 1:3, 1:4, 1:5, 1:10, 1:20, 1:30, 1:40 or 1:50.

[0015] According to an embodiment of the present application, the total mass of the compound I and the compound II in the composition is 1-80%, preferably 2-70%, or 5-65%, 10-60%, for example 1%, 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45% or 50%, based on the total weight of the composition.

[0016] According to an embodiment of the present application, the composition can be prepared into a liquid preparation or a solid preparation.

[0017] According to an embodiment of the present application, the content of the effective active ingredient in the preparation varies according to different preparation types, generally, the liquid preparation contains 1-60% of the effective active ingredient by weight, preferably 5-50%; the solid preparation contains 5-80% of the effective active ingredient by weight, preferably 10-70%.

[0018] According to the embodiments of the present application, at least one of deionized water, organic solvent, emulsifier, dispersant, wetting agent, thickening agent, antifoaming agent, stabilizer, binder, disintegrant, anti-freezing agent, anti-caking agent, suspending agent, film forming agent, preservative, colorant, high molecular capsule wall material, pH adjusting agent or filler, etc. is further included in the composition.

[0019] According to the embodiments of the present application, the composition can be diluted by the user before use or used directly. Its preparation can be prepared by mixing one or more of the effective active ingredients with deionized water, organic solvent, emulsifier, dispersant, wetting agent, thickening agent, antifoaming agent, stabilizer, binder, disintegrant, anti-freezing agent, anti-caking agent, suspending agent, film forming agent, preservative, colorant, high molecular capsule wall material, pH adjusting agent or filler, etc. to prepare the composition.

[0020] According to the preparation provided by the present application, the composition can be made into various dosage forms, preferably, the dosage forms include wettable powder, water dispersible granule, suspension, emulsion, suspension seed coating, microcapsule suspension, microcapsule suspension-suspension, emulsion, microemulsion, dispersible liquid, granule.

[0021] According to the embodiments of the present application, when the composition is prepared into wettable powder, it includes the following components and contents: compound I 0.1% to 80%, compound II 0.1% to 75%, dispersant 0% to 10%, wetting agent 0% to 8%, and the rest is filler.

[0022] Preferably, the compound I in the composition is 2% to 70%, 5% to 65%, 10% to 60%, 15% to 50%, etc.

[0023] The compound II is 2% to 70%, 5% to 65%, 10% to 60%, 15% to 50%, etc.

[0024] The dispersant is 1% to 8%, 2% to 7%, 3-6% or 4% to 5%;

[0025] The wetting agent is 1% to 8%, 2% to 7%, 3-6% or 4% to 5%.

[0026] According to the embodiments of the present application, when the composition is prepared into water dispersible granule, it includes the following components and contents: compound I 0.1% to 80%, compound II 0.1% to 75%, dispersant 0% to 10%, wetting agent 0% to 16%, disintegrant 0% to 20%, and the rest is filler.

[0027] Preferably, the compound I in the composition is 2% to 70%, 5% to 65%, 10% to 60%, 15% to 50%, etc.

[0028] Compound II is 2 to 70%, 5 to 65%, 10 to 60%, 15 to 50%, etc.

[0029] The dispersing agent is 1 to 8%, 2 to 7%, 3-6%, or 4 to 5%;

[0030] The wetting agent is 1 to 8%, 2 to 7%, 3-6%, or 4 to 5%;

[0031] The disintegrating agent is 1 to 18%, 2 to 16%, 3-15%, 4 to 12%, 5 to 10%, or 6-8%.

[0032] According to an embodiment of the present application, the composition, when made into a flowable, includes the following components and amounts: Compound I 0.1 to 50%, Compound II 0.1 to 50%, dispersing agent 0 to 5%, wetting agent 0 to 8%, thickening agent 0 to 0.3%, anti-freeze agent 0 to 5%, and the balance deionized water.

[0033] Preferably, Compound I in the composition is 2 to 45%, 5 to 40%, 10 to 35%, 15 to 30%, 20 to 25%, etc.

[0034] Compound II is 2 to 45%, 5 to 40%, 10 to 35%, 15 to 30%; 20 to 25%, etc.

[0035] The dispersing agent is 1 to 4% or 2 to 3%;

[0036] The wetting agent is 1 to 8%, 2 to 7%, 3-6%, or 4 to 5%;

[0037] The thickening agent is 0.1 to 0.25%;

[0038] The anti-freeze agent is 1 to 4% or 2 to 3%.

[0039] According to an embodiment of the present application, the composition, when made into an emulsion, includes the following components and amounts: Compound I 0.1 to 50%, Compound II 0.1 to 50%, organic solvent 0 to 95%, emulsifier 0 to 10%, anti-freeze agent 0 to 5%, anti-foaming agent 0 to 0.2%, thickening agent 0 to 0.5%, and the balance deionized water.

[0040] Preferably, Compound I in the composition is 2 to 45%, 5 to 40%, 10 to 35%, 15 to 30%, 20 to 25%, etc.

[0041] Compound II is 2 to 45%, 5 to 40%, 10 to 35%, 15 to 30%; 20 to 25%, etc.

[0042] The organic solvent is 1-90%, 2-80%, 5-70%, 10-60%, 15-50%; 20-50%, 30-40%, etc.

[0043] The emulsifier is 1-8%, 2-7%, 3-6%, or 4-5%;

[0044] The anti-freezing agent is 1-4% or 2-3%;

[0045] The defoaming agent is 0.05-0.1%, or

[0046] The thickening agent is 0.1-0.4%, 0.2-0.3%.

[0047] According to an embodiment of the present application, when the composition is made into a flowable concentrate, it comprises the following components and contents: Compound I 0.1-50%, Compound II 0.1-50%, dispersing agent 1-12%, wetting agent 1-10%, anti-freezing agent 1-10%, anti-caking agent 0.1-10%, suspending agent 0.1-5%, film-forming agent 1-10%, preservative 0.1-5%, colorant 1-30%, pH regulator 0.1-5%, thickening agent 0.1-8%, and the balance is deionized water.

[0048] Preferably, Compound I in the composition is 2-45%, 5-40%, 10-35%, 15-30%, 20-25%, etc.

[0049] Compound II is 2-45%, 5-40%, 10-35%, 15-30%; 20-25%, etc.

[0050] The dispersing agent is 2-10%, 3-8%, 4-7%, or 5-6%;

[0051] The wetting agent is 1-8%, 2-7%, 3-6%, or 4-5%;

[0052] The anti-freezing agent is 1-8%, 2-7%, 3-6%, or 4-5%;

[0053] The anti-caking agent is 0.5-9%, 1-8%, 2-7%, 3-6%, or 4-5%;

[0054] The suspending agent is 0.2-4%, 0.5-3.5%, 1-3%, 1.5-2.5%, or 1.8-2%;

[0055] The film-forming agent is 2-9%, 3-8%, 4-7%, or 5-6%;

[0056] The preservative is 0.2-4%, 0.5%-3.5%, 1%-3%, 1.5%-2.5%, or 1.8%-2%;

[0057] The colorant is 2-25%, 3%-20%, 5%-15%, 8%-12%, or 9%-10%;

[0058] The pH regulator is 0.1%-5%, 0.2-4%, 0.5%-3.5%, 1%-3%, 1.5%-2.5%, or 1.8%-2%;

[0059] The thickening agent is 0.5%-7, 1%-6%, 2%-5%, or 3%-4%.

[0060] According to an embodiment of the present application, when the composition is made into a microcapsule suspension agent, it comprises the following components and contents: Compound I 0.1%-50%, Compound II 0.1%-50%, high molecular capsule wall material 1-30%, dispersant 2%-10%, organic solvent 1-50%, emulsifier 1%-7%, pH regulator 0.1%-5%, antifoaming agent 0.01%-2%, thickening agent 0.1%-8%, antifreezing agent 0.1%-8%, and the balance is deionized water.

[0061] Preferably, Compound I in the composition is 2%-45%, 5%-40%, 10%-35%, 15%-30%, 20%-25%, etc.

[0062] Compound II is 2%-45%, 5%-40%, 10%-35%, 15%-30%, 20%-25%, etc.

[0063] The high molecular capsule wall material is 2-25%, 5-20%, 6-18%, or 10-15%, etc.

[0064] The dispersant is 3%-8%, 4%-7%, or 5%-6%;

[0065] The organic solvent is 2%-45%, 5%-40%, 10%-35%, 15%-30%, or 20%-25%, etc.

[0066] The emulsifier is 2%-6% or 4%-5%;

[0067] The pH regulator is 0.2-4%, 0.5%-3.5%, 1%-3%, 1.5%-2.5%, or 1.8%-2%;

[0068] The antifoaming agent is 0.05-1.8%, 0.1%-1.5%, 0.5%-1.3%, or 0.8%-1.0%;

[0069] The thickener is 0.2%–7%, 0.5%–6%, 1%–5%, 1.5%–4%, or 2%–3%;

[0070] The antifreeze is 0.5%–7%, 1%–6%, 2%–5%, or 3%–4%.

[0071] According to an embodiment of the present invention, when the composition is prepared into a microcapsule suspension, it comprises the following components and contents: compound I 0.1%–50%, compound II 0.1%–50%, polymeric capsule wall material 1%–12%, dispersant 1%–12%, wetting agent 1%–8%, organic solvent 1%–50%, emulsifier 1%–8%, defoamer 0.01%–2%, thickener 0.1%–8%, pH adjuster 0.1%–5%, antifreeze agent 0.1%–8%, and the balance being deionized water.

[0072] According to an embodiment of the present invention, when the composition is prepared into an emulsifiable concentrate, it comprises the following components and contents: compound I 0.1% to 50%, compound II 0.1% to 50%, emulsifier 0% to 10%, antifreeze 0% to 5%, stabilizer 0% to 0.5%, and the balance being an organic solvent;

[0073] Alternatively, compound I 0.1%–50%, compound II 0.1%–50%, emulsifier 0–12%, stabilizer 0%–0.5%, and the balance being organic solvent.

[0074] According to an embodiment of the present invention, when the composition is prepared into a microemulsion, it comprises the following components and contents: compound I 0.1% to 50%, compound II 0.1% to 50%, organic solvent 1% to 50%, emulsifier 1% to 30%, antifreeze 1% to 10%, stabilizer 0.1% to 5%, and the balance being deionized water.

[0075] According to an embodiment of the present invention, when the composition is prepared into a dispersible liquid, it comprises the following components and contents: compound I 0.1% to 50%, compound II 0.1% to 50%, emulsifier 1% to 30%, antifreeze 1% to 10%, stabilizer 0.1% to 5%, and the balance being an organic solvent.

[0076] According to an embodiment of the present invention, when the composition is made into granules, it comprises the following components and contents: compound I 0.1% to 10%, compound II 0.1% to 10%, dispersant 0% to 5%, wetting agent 0% to 5%, binder 0% to 3%, and the balance being filler.

[0077] According to an embodiment of the present application, the emulsifier can be selected from at least one of sodium lignosulfonate, Agrimul, phenylphenol polyoxyethyl ether phosphate, triphenyl ethyl styryl phenol polyoxyethylene ether phosphate triethanolamine salt, benzyl dimethyl phenol polyoxyethyl ether, sorbitan fatty acid ester polyoxyethylene ether, sorbitan oleate (Span-80), fatty alcohol polyoxyethylene ether, sodium alkyl naphthalene sulfonate, sodium isooctanol succinate sulfonate, nonyl phenol polyoxyethylene ether phosphate, castor oil polyoxyethylene ether phosphate.

[0078] According to an embodiment of the present application, the dispersant can be selected from at least one of glycerin fatty acid polyoxyethylene ether, polyoxyethylene alkyl aryl ether, sodium lignosulfonate, naphthalene sulfonate formaldehyde condensate, fatty alcohol polyoxyethylene ether sulfate, naphthalene sulfonate formaldehyde condensate, naphthalene sulfonate formaldehyde condensate sodium salt, nonyl phenol polyoxyethylene ether, polyoxyethylene lanolin alcohol, alkyl phenol polyoxyethylene ether formaldehyde condensate, fatty alcohol polyoxyethylene ether, fatty alcohol polyoxyethylene ether phosphate, polyoxyethylene sorbitan fatty acid ester, phosphate ester.

[0079] According to an embodiment of the present application, the wetting agent can be selected from at least one of trisiloxane polyoxyethylene ether, sodium N-lauroyl glutamate, sodium dodecyl sulfate, sodium lauroyl sarcosinate, sodium methyl naphthalene sulfonate formaldehyde condensate, castor oil polyoxyethylene ether, triphenyl ethyl phenol polyoxyethylene ether, sodium dodecyl benzene sulfonate, sodium alkyl naphthalene sulfonate, sodium isooctanol succinate sulfonate, polyoxyethylene alkyl aryl ether, fatty alcohol polyether glycerin fatty acid polyoxyethylene ether, fatty alcohol polyoxyethylene ether.

[0080] According to an embodiment of the present application, the binder can be selected from at least one of xanthan gum, starch, urea formaldehyde resin, gelatin, gum arabic, carboxymethyl cellulose, carboxyethyl cellulose, polyvinyl alcohol.

[0081] According to an embodiment of the present application, the disintegrant can be selected from at least one of sodium bicarbonate, ammonium sulfate, sodium sulfate, calcium sulfate, magnesium chloride.

[0082] According to an embodiment of the present application, the thickening agent can be selected from at least one of magnesium aluminum silicate, polyvinyl acetate, xanthan gum, gelatin, gum arabic, polyvinyl alcohol.

[0083] According to an embodiment of the present application, the antifoaming agent can be selected from at least one of silicone oil, n-octanol, silicone, butyl phosphate, isobutyl phosphate, etc.

[0084] According to an embodiment of the present application, the antifreeze agent can be selected from at least one of propylene glycol, ethylene glycol, glycerol, etc.

[0085] According to an embodiment of the present application, the stabilizer can be selected from at least one of triethanolamine, epichlorohydrin, butyl glycidyl ether, triphenyl phosphite, N-soybean oil-based triethylene diamine, dialkyl succinate acetosulfate, etc.

[0086] According to an embodiment of the present application, the filler includes a solid filler and a liquid filler, wherein the solid filler can be selected from at least one of kaolin, attapulgite, diatomite, white carbon, bentonite, montmorillonite, calcium carbonate, talc powder. The liquid filler can be selected from at least one of soybean oil, castor oil, mineral oil.

[0087] According to an embodiment of the present application, the organic solvent can be selected from at least one of ethyl acetate, acetone, isopropyl alcohol, 2,2,2-trifluoroethanol, propylene carbonate, benzene, toluene, xylene, dimethylformamide, dimethyl sulfoxide, dichloromethane, cyclohexane, cyclohexanone, N-methyl pyrrolidone, solvent oil (e.g., 150# solvent oil).

[0088] The present application also provides a use of the above composition for controlling pests in agriculture and non-agriculture.

[0089] According to an embodiment of the present application, the pests are Lepidoptera, Hemiptera, Coleoptera, Thysanoptera, Diptera, mites, etc.

[0090] As an embodiment, the pests are selected from the following: cotton aphids, corn armyworms.

[0091] The present application also provides a method for controlling pests in agriculture or non-agriculture, comprising applying the above composition to plants where pests exist.

[0092] The composition of the present application can be provided in the form of a finished preparation, or in the form of a single agent, which is directly mixed before use, and then diluted with water to the desired concentration, and can be applied to crops in any way, such as spraying, plant root irrigation, smearing, etc. In specific applications, it can also be mixed with other agents such as growth regulators, soil conditioners, herbicides, nematicides, etc.

[0093] The present application also provides a composition for controlling invertebrate pests, which comprises a biologically effective amount of Compound I, Compound II and at least one other component selected from the group consisting of surfactants, solid diluents and liquid diluents, said composition optionally further containing an effective amount of at least one other biologically active compound or active agent, said Compound II being selected from at least one of the following compounds: abamectin, emamectin benzoate, pymetrozine, chlorfenapyr, methoxyfenozide, tebufenozide.

[0094] The present application also provides a method for controlling an invertebrate pest, comprising contacting said invertebrate pest or its environment with a biologically effective amount of the composition of the present application as described above.

[0095] According to an embodiment of the present application, the invertebrate pest is selected from cotton aphid, corn aphid or corn earworm.

[0096] The present application also provides a spray composition comprising the composition of the present application as described above and a propellant.

[0097] The present application also provides a bait composition comprising the composition of the present application as described above; one or more food materials; optionally an attractant; and optionally a wetting agent.

[0098] Advantages of the present application:

[0099] The synergistic composition of the present application has the following advantages:

[0100] 1) synergistic effect, improving the control effect of pests; 2) expanding the insecticidal spectrum, mixed occurrence of field pests, stronger effect on pests; 3) different action mechanisms of the two active ingredients, mixed use can delay the development of pest resistance; 4) reducing the amount of pesticide application, reducing the use frequency and reducing the use cost. DETAILED DESCRIPTION

[0101] The technical solutions of the present application will be further described in detail below in combination with specific examples. It should be understood that the following examples are only illustrative and explanatory of the present application, and should not be interpreted as limiting the scope of protection of the present application. Any technology realized based on the above description of the present application is included in the scope of protection intended by the present application.

[0102] Unless otherwise specified, the raw materials and reagents used in the following examples are commercially available or can be prepared by known methods.

[0103] The present application uses a combination of indoor bioassay and field test to test the insecticidal effect of the composition. It should be noted that any modification, equivalent replacement and improvement within the spirit and principles of the present application should be included in the scope of protection of the present application.

[0104] The compound I used in the following examples was prepared according to the method of Example 1 in the patent document CN 111909143 A.

[0105] The % of each component in the preparation of the following examples, if not specifically stated, refers to the mass percentage content.

[0106] Preparation Example 1: Wettable powder

[0107]

[0108] The above materials are roughly crushed in proportion, mixed uniformly in a mixer, and then air-ground to obtain the finished product.

[0109] Preparation Example 2: Water dispersible granules

[0110]

[0111] The effective active ingredient and various adjuvants are mixed uniformly in proportion to the formula, air-ground to obtain a powder, and then a certain amount of water is added to mix and extrude granulation, dried and sieved to obtain the finished product.

[0112] Preparation Example 3: Suspension concentrate

[0113]

[0114] The effective active ingredient and various adjuvants are mixed uniformly in proportion to the formula, high-speed sheared and sand-milled to obtain the finished product.

[0115] Preparation Example 4: Emulsion

[0116]

[0117] The above raw materials are mixed in proportion to make them dissolve into a uniform oil phase; after passing the inspection, they are measured and packaged to obtain the finished product.

[0118] Preparation Example 5: Water emulsion

[0119]

[0120]

[0121] The above raw materials are added to a batching kettle according to the formula requirements, mixed uniformly by a high-speed shearing machine to obtain a water emulsion, which is measured and packaged after passing the inspection to obtain the finished product.

[0122] Preparation Example 6: Emulsion

[0123]

[0124] The above raw materials are mixed in proportion to make them dissolve into a uniform oil phase; after passing the inspection, they are measured and packaged to obtain the finished product.

[0125] Preparation Example 7: Granules

[0126]

[0127] The components are mixed in proportion according to the formula, crushed, wetted with water, and then fully stirred to obtain the finished product.

[0128] In the preparation example above, compound II is selected from at least one of abamectin, emamectin benzoate, pymetrozine, chlorfenapyr, methoxyfenozide, or tebufenozide.

[0129] II. Examples of Indoor Toxicity Testing

[0130] (1) Tested pests: cotton aphid (Aphis gossypii Glover) and corn armyworm (Mythimnaseparata Walker).

[0131] (2) Measurement method:

[0132] Methods for determining the toxicity of pests

[0133] The toxicity of the pest was determined using cotton aphids as the test material, and the toxicity of compound I, compound II and their mixtures was determined by immersion method.

[0134] Referring to the People's Republic of China Agricultural Industry Standard NY / T 1154.6-2006 "Guidelines for Indoor Bioassay Testing of Pesticides," Part 6: Insecticide Activity Test - Immersion Method, the test agents (including Compound I and Compound II) were first prepared into five concentration gradients using suitable solvents (the type of solvent is such as acetone, methanol, N,N-dimethylformamide, and dimethyl sulfoxide, etc., selected according to their solubility in the sample). 50 (The concentration gradient is set according to the ratio or arithmetic progression), and the compounds I and II to be mixed are respectively calculated according to their LC values. 50 The values ​​are set according to a series of proportions based on mass ratio, and the final mass concentration (referring to the total mass of compound I and compound II) is prepared by different ratios.

[0135] After immersing the target insects in the drug solution for 5-10 seconds, excess solution is absorbed with filter paper, and the insects are transferred to normal rearing conditions. Each treatment is repeated in 4 replicates, with 10-20 insects immersed in each replicate. A corresponding organic solvent treatment without the drug is included as a control.

[0136] The toxicity of the pest was determined using the corn armyworm as the test material, and the toxicity of compound I, compound II and their mixtures was determined by the leaf immersion method.

[0137] Referring to the People's Republic of China Agricultural Industry Standard NY / T 1154.14-2008 "Guidelines for Indoor Bioassay Testing of Pesticides," Insecticides Part 14: Leaf Dipping Method, the test agents (including Compound I and Compound II) were first prepared into five concentration gradients using suitable solvents (the type of solvent, such as acetone, methanol, N,N-dimethylformamide, and dimethyl sulfoxide, etc., selected according to their solubility in the sample). 50The concentration gradient is set according to the geometric or arithmetic progression, and the compound I and the compound II for mixing are respectively prepared according to the LC 50 The series of proportions are set according to the mass proportion, and the final mass concentration (referring to the total mass of the compound I and the compound II) is prepared according to different proportions.

[0138] The leaf discs or leaf segments are immersed in the solution of the agent to be tested, taken out after 10s (the immersion time can be appropriately prolonged or shortened according to the characteristics of the agent), dried and placed in a culture dish containing 1% water agar or a moisture filter paper, and the test insects are introduced, with not less than 10 insects per repeat. Each treatment has not less than 4 repeats, and a treatment without the agent (containing all organic solvents and emulsifiers) is set as a blank control.

[0139] The co-toxicity coefficients (CTC values) of the two agents in different proportions are calculated according to the method of Sun Yunpei. The co-toxicity coefficient (CTC) of the mixed agent ≥120 indicates synergistic effect, the CTC ≤80 indicates antagonistic effect, and 80<CTC<120 indicates additive effect. The co-toxicity coefficient (CTC) of the mixed agent is calculated according to formula (1), formula (2) and formula (3):

[0140]

[0141] In the formula:

[0142] ATI - the measured toxicity index of the mixed agent;

[0143] S - the LC 50 of the standard insecticide, with the unit of milligrams per liter (mg / L);

[0144] M - the LC 50 of the mixed agent, with the unit of milligrams per liter (mg / L)

[0145] TTI = TI A × P A + TI B × P B (2)

[0146] In the formula:

[0147] TTI - the theoretical co-toxicity index of the mixed agent;

[0148] TI A - the toxicity index of the agent A;

[0149] P A - the percentage content of the agent A in the mixed agent, with the unit of percentage (%);

[0150] TI B - the toxicity index of the agent B;

[0151] P BThe percentage content of the B agent in the mixture, in percentage (%).

[0152]

[0153] In the formula:

[0154] CTC - coefficient of toxicity;

[0155] ATI - actual toxicity index of the mixture;

[0156] TTI - theoretical toxicity index of the mixture.

[0157] When different pesticides are mixed, they usually exhibit three types of action, namely, additive action, synergistic action and antagonistic action, but it is impossible to predict which type of action will occur. The composition described in the present application uses compound I and compound II as effective active ingredients, and is illustrated by the biological determination examples.

[0158] Laboratory activity determination test of the insecticide on cotton aphids

[0159] In this test, the insecticidal activity of compound on cotton aphids was determined by the insect immersion method, in order to determine the activity of the insecticidal composition of Example 1 on cotton aphids.

[0160] Example 1: In order to verify the effect of different proportions of compound I and pymetrozine on cotton aphids, the indoor toxicity determination of different proportions of compound I and pymetrozine on cotton aphids was carried out, and the corresponding concentration was prepared according to the weight ratio in the table, and the indoor insecticidal test was carried out, and the test results are as follows:

[0161] Table 1 Toxicity determination results of compound I and pymetrozine single agent and their mixture on cotton aphids

[0162]

[0163]

[0164] From the results in Table 1, it can be seen that the activity of different proportions of compound I and pymetrozine mixed for cotton aphids has obvious synergistic effect.

[0165] Laboratory activity determination test of the insecticide on corn armyworms

[0166] In this test, the insecticidal activity of compound on corn armyworms was determined by the leaf immersion method, in order to determine the activity of the insecticidal composition of Examples 2-6 on corn armyworms.

[0167] Example 2: In order to verify the effect of different proportions of compound I and emamectin benzoate on corn armyworms, the indoor toxicity determination of different proportions of compound I and emamectin benzoate on corn armyworms was carried out, and the corresponding concentration was prepared according to the weight ratio in the table, and the indoor insecticidal test was carried out, and the test results are as follows:

[0168] Table 2 Toxicity test results of compound I and emamectin benzoate single agent and their mixtures on corn earworm

[0169] Active ingredient Ratio Regression equation LC 50 ]]> Co-toxicity coefficient Evaluation Compound I - y = 5.7178 + 2.0073x 0.4389 - - emamectin benzoate - y = 6.5581 + 1.9294x 0.1558 - Compound I: emamectin benzoate 50:1 y = 5.9553 + 1.8220x 0.2990 141.7412 Synergism Compound I: emamectin benzoate 10:1 y = 6.0765 + 1.5206x 0.1959 192.2831 Synergism Compound I: emamectin benzoate 5:1 y = 6.2890 + 1.5848x 0.1537 219.1687 Synergism Compound I: emamectin benzoate 3:1 y = 6.4830 + 1.6914x 0.1328 227.2600 Synergism Compound I: emamectin benzoate 1:1 y = 6.8366 + 1.8013x 0.0956 240.5510 Synergism Compound I: emamectin benzoate 1:3 y = 7.3846 + 2.0332x 0.0672 276.4194 Synergism Compound I: emamectin benzoate 1:5 y = 7.6015 + 2.2582x 0.0705 247.6181 Synergism Compound I: emamectin benzoate 1:10 y = 7.3405 + 2.0374x 0.0710 233.1041 Synergism Compound I: emamectin benzoate 1:50 y = 7.7155 + 2.4681x 0.0794 198.7341 Synergism

[0170] From the results in Table 2, it can be seen that the activity of compound I and emamectin benzoate mixed in different proportions has obvious synergistic effect on corn earworm.

[0171] Example 3: To verify the effect of compound I and buprofezin mixed in different proportions on corn earworm, indoor toxicity test of compound I and buprofezin mixed in different proportions on corn earworm was carried out, and indoor insecticidal test was carried out according to the corresponding concentration prepared according to the weight ratio in the table, and the test results were as follows:

[0172] Table 3 Toxicity test results of compound I and buprofezin single agent and their mixtures on corn earworm

[0173]

[0174]

[0175] From the results in Table 3, it can be seen that the activity of compound I and buprofezin mixed in different proportions has obvious synergistic effect on corn earworm.

[0176] Example 4: To verify the effect of compound I and methoxyfenozide mixed in different proportions on corn earworm, indoor toxicity test of compound I and methoxyfenozide mixed in different proportions on corn earworm was carried out, and indoor insecticidal test was carried out according to the corresponding concentration prepared according to the weight ratio in the table, and the test results were as follows:

[0177] Table 4 Toxicity test results of compound I and methoxyfenozide single agent and their mixtures on corn earworm

[0178]

[0179] From the results in Table 4, it can be seen that the activity of compound I and methoxyfenozide mixed in different proportions has obvious synergistic effect on corn earworm.

[0180] Example 5: To verify the effect of compound I and tebufenozide mixed in different proportions on corn earworm, indoor toxicity test of compound I and tebufenozide mixed in different proportions on corn earworm was carried out, and indoor insecticidal test was carried out according to the corresponding concentration prepared according to the weight ratio in the table, and the test results were as follows:

[0181] Table 5 Toxicity test results of compound I and tebufenozide single agent and their mixtures on corn earworm

[0182]

[0183] From the results of Table 5, it can be seen that the different mixing ratios of compound I and tebufenozide have obvious synergistic effects on the activity against O. nubilalis.

[0184] Example 6: To verify the effects of different mixing ratios of compound I and abamectin on O. nubilalis, indoor toxicity tests of different mixing ratios of compound I and abamectin on O. nubilalis were carried out. The corresponding concentrations were prepared according to the weight ratio in the table, and indoor insecticidal tests were carried out. The test results are as follows:

[0185] Table 6 Toxicity test results of compound I and abamectin single agent and their mixtures on O. nubilalis

[0186]

[0187]

[0188] From the results of Table 6, it can be seen that the different mixing ratios of compound I and abamectin have obvious synergistic effects on the activity against O. nubilalis.

[0189] Three, field efficacy examples

[0190] In order to clarify the effects of compound I and compound II single agent and mixture on cotton aphids and O. nubilalis, multiple efficacy tests were carried out in different regions of China. The preparations were processed according to Preparation Examples 1-7, and the preparation in Table 7 was taken as an example (other test preparations refer to the same preparation example). The specific preparation method is as follows:

[0191] Table 7 Preparation of different mixing ratios of compound I and pymetrozine suspensions

[0192]

[0193] Cotton aphid field efficacy test

[0194] Test method: According to the application method specified in the "People's Republic of China National Standard GB / T 17980.75-2004", when the aphid cotton plant reaches more than 20%, the pesticide is applied.

[0195] Investigation method: According to the investigation method specified in the "People's Republic of China National Standard GB / T 17980.75-2004", after 4 leaf stage (true leaf) of cotton, 5 points were sampled per plot, and 2 plants were fixed per point. Each plant has 1-2 aphid leaves in the middle, and the number of aphids on the fixed leaves is investigated. The initial population is investigated before treatment, and the number of live insects is investigated 1, 3, and 7 days after treatment, and the control effect (control effect) is calculated. The control effect calculation formula is:

[0196]

[0197]

[0198] Field efficacy test of corn borer

[0199] Test method: The application method is according to the method prescribed in the National Standard of the People's Republic of China GB / T 17980.80-2004. The application is performed when the larvae are at the 2nd and 3rd instar.

[0200] Investigation method: The investigation method is according to the method prescribed in the National Standard of the People's Republic of China GB / T 17980.80-2004. 5 points are sampled in each plot, and 1 m 2 , investigation is performed before application, and 1, 3, and 7 days after application, and the control effect is calculated. The control effect calculation formula is:

[0201]

[0202]

[0203] Table 8 Field efficacy test of compound I and pymetrozine on cotton aphids

[0204]

[0205]

[0206] Note: Different letters in the same column represent significant difference at the level of P<0.05 by Duncan's new complex range test, and the same below.

[0207] The above example 7 10% compound I pymetrozine suspension (1:4) is prepared according to the preparation example 3, wherein 10% refers to the total mass percentage of active substances (compound I and pymetrozine), and 1:4 refers to the mass ratio of the two active ingredients compound I and pymetrozine.

[0208] The 10% compound I pymetrozine suspension (1:3) in example 8 is prepared according to the preparation example 3, wherein 10% refers to the total mass percentage of active substances (compound I and pymetrozine), and 1:3 refers to the mass ratio of the two active ingredients compound I and pymetrozine.

[0209] The 10% compound I suspension in comparative example 1 is also prepared according to the preparation example 3, wherein 10% refers to the mass percentage of active substance compound I. The following other examples and comparative examples are also prepared in this way, which will not be described in detail here.

[0210] From the results of the table, in the prevention and treatment of cotton aphids, compound I and pymetrozine complex is significantly higher than single agent in 1 day, 3 days and 7 days control effect of cotton aphids at the same effective ingredient dosage (comparative example 1 and 2 corresponding to example 7-13). Among them, the 7-day control effect can still reach more than 92.81%, and the effective period is longer.

[0211] Table 9 Field efficacy test of compound I and chlorfenapyr complex on corn armyworm

[0212]

[0213]

[0214] From the results of the table, in the prevention and treatment of corn armyworm, compound I and chlorfenapyr complex is significantly higher than single agent in 1 day, 3 days and 7 days control effect of corn armyworm at the same effective ingredient dosage (comparative example 3 and 4 corresponding to example 14-20). Among them, the 7-day control effect can still reach more than 94.47%, and the effective period is longer.

[0215] Table 10 Field efficacy test of compound I and emamectin benzoate on cotton aphids

[0216]

[0217] From the results of the table, in the prevention and treatment of cotton aphids, compound I and emamectin benzoate complex is significantly higher than single agent in 1 day, 3 days and 7 days control effect of cotton aphids at the same effective ingredient dosage (comparative example 4 and 5 corresponding to example 21-27). Among them, the 7-day control effect can still reach more than 92.98%, and the effective period is longer.

[0218] Table 11 Field efficacy test of compound I and emamectin benzoate complex on corn armyworm

[0219]

[0220]

[0221] From the results of the table, in the prevention and treatment of corn armyworm, compound I and emamectin benzoate complex is significantly higher than single agent in 1 day, 3 days and 7 days control effect of corn armyworm at the same effective ingredient dosage (comparative example 6 and 7 corresponding to example 28-34). Among them, the 7-day control effect can still reach more than 95.33%, and the effective period is longer.

[0222] The above describes the embodiments of the present application. However, the present application is not limited to the above-described embodiments. Any modification, equivalent replacement, improvement, and the like made within the spirit and principle of the present application should be included in the scope of protection of the present application.

Claims

1. A composition comprising Compound I and Compound II, characterized in that, said compound II is chlorfenapyr; said compound I has the structure I； said compound I and compound II are in a mass ratio of 50-1:1-50.

2. The composition of claim 1, wherein, said compound I and compound II are in a mass ratio of 10-1:1-10.

3. The composition of claim 1, wherein, said compound I and compound II are in a mass ratio of 50:1, 40:1, 30:1, 20:1, 10:1, 5:1, 4:1, 3:1, 2:1, 1:1, 1:2, 1:3, 1:4, 1:5, 1:10, 1:20, 1:30, 1:40 or 1:

50.

4. The composition according to any one of claims 1 to 3, characterized in that, at least one of deionized water, organic solvent, emulsifier, dispersant, wetting agent, thickening agent, antifoaming agent, stabilizer, binder, disintegrant, anti-freezing agent, anti-caking agent, suspending agent, film forming agent, preservative, colorant, high molecular capsule wall material, pH adjustor or filler is further included in the composition.

5. The composition according to any one of claims 1 to 3, characterized in that, said composition is formulated into a pesticide dosage form, which includes wettable powder, water dispersible granule, suspension concentrate, emulsion in water, suspension seed coating, microcapsule suspension concentrate, microcapsule suspension-suspension concentrate, emulsion in oil, microemulsion, dispersible liquid or granule.

6. Use of the composition of any one of claims 1-5 for controlling insect pests in agriculture or non-agriculture.

7. Use according to claim 6, characterized in that, said insect pests are selected from Lepidoptera, Hemiptera, Coleoptera, Thysanoptera, Diptera, mite pests.

8. A method of controlling pests in agriculture or non-agriculture, characterized by, comprising applying the composition of any one of claims 1-5 to a plant where the insect pests are present.

9. A composition for controlling an invertebrate pest, characterized by comprising the compound of claim 1. which comprises a biologically effective amount of compound I, compound II which is chlorfenapyr, and at least one additional component selected from the group consisting of surfactants, solid diluents and liquid diluents; said compound I has the structure I said compound I and compound II are in a mass ratio of 50-1:1-50.

10. A method for controlling an invertebrate pest, comprising contacting the pest with a composition comprising a compound of the formula (I) as defined in any one of claims 1 to 9. comprising contacting an invertebrate pest or its environment with a biologically effective amount of the composition of any one of claims 1-5.

11. A spray composition comprising the composition of any one of claims 1-5 and a propellant.

12. A bait composition comprising the composition of any one of claims 1-5; and one or more food materials.

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