Mouthwash containing natural plant essential oil composition and preparation method thereof

Abstract

The present invention discloses a mouthwash containing a natural plant essential oil composition and a preparation method thereof, and relates to the technical field of oral care products. The mouthwash contains a natural plant essential oil composition, which is composed of a compound essential oil and a nonionic surfactant. The compound essential oil is composed of peppermint essential oil, eucalyptus essential oil, ginger essential oil, methyl salicylate, lemon essential oil and Melaleuca alternifolia oil; the nonionic surfactant is composed of poloxamer 407, polysorbate 20 and PEG 60 hydrogenated castor oil. The present invention realizes the introduction of oil-soluble active substances into aqueous mouthwashes by synergistically enhancing the effect of compound essential oils by wrapping them with nonionic surfactants. On the one hand, the excellent transdermal absorbability of natural plant essential oils is utilized to achieve antibacterial, anti-inflammatory and antioxidant effects. On the other hand, a safe and non-toxic nonionic surfactant is introduced to promote the uniform dispersion of oil-soluble substances in water.

Description

Technical Field

[0001] The present invention relates to the technical field of oral care products, in particular to a mouthwash containing a natural plant essential oil composition and a preparation method thereof. Background Art

[0002] Mouthwash can reach areas of the mouth that are often overlooked when brushing, such as the gaps between teeth, the tongue coating, and the oral mucosa, achieving a comprehensive oral cleansing effect. In Europe and the United States, the mouthwash market is almost on par with toothpaste, but in my country, mouthwash still occupies a relatively small share. After continuous market education and penetration, for example, some restaurants and hotels have gradually introduced mouthwash to the public, which has subtly changed people's oral care habits. Daily use of mouthwash is more convenient for people to communicate and have close contact. Currently, research on the compositions in mouthwashes has mostly focused on anti-caries compositions containing fluoride, antibacterial compositions containing cations or biguanides, and anti-sensitivity compositions containing ingredients such as potassium nitrate. However, the use of these types of mouthwashes has certain limitations, and it is necessary to consult a dentist before use. There is also a related patent CN106176315B that conducts related research on compound essential oils. Because plant essential oils are all naturally derived, have good permeability, have a certain impact on plaque biofilms, and have good antibacterial properties, the use of plant essential oils is a new direction in mouthwash research. Currently studied plant essential oil mouthwashes mostly use a high alcohol system. Patent CN104784240B proposes the use of alcohol to assist in solubilization to achieve its dissolution in aqueous mouthwashes. However, studies have shown that high alcohol concentrations are highly irritating to both the mouth and the skin. Introducing plant essential oils on this basis will aggravate the damage to the oral mucosa and is not conducive to wound healing in patients with oral ulcers. Summary of the Invention

[0003] To address the solubility issues of plant essential oils in mouthwashes while maintaining both efficacy and mildness, the present invention provides a mouthwash containing a natural plant essential oil composition and a preparation method thereof. Specifically, the present invention includes the following technical solutions:

[0004] In a first aspect, a mouthwash containing a natural plant essential oil composition is provided. The mouthwash contains the natural plant essential oil composition, wherein the natural plant essential oil composition is composed of a compound essential oil and a non-ionic surfactant, wherein the compound essential oil is composed of peppermint essential oil, eucalyptus essential oil, ginger essential oil, methyl salicylate, lemon essential oil, and Melaleuca alternifolia oil; and the non-ionic surfactant is composed of poloxamer 407, polysorbate 20, and PEG-60 hydrogenated castor oil.

[0005] Among them, peppermint essential oil has a good penetration-promoting effect and has a good affinity with the lipid bilayer of the stratum corneum, thereby promoting the transdermal absorption of drugs; eucalyptus essential oil is a mixture mainly composed of terpenes, which can reduce the expression of pro-inflammatory factors and alleviate the inflammatory response by interfering with the signal pathway of the inflammatory mechanism; the main volatile components of ginger essential oil include curcumin, cyclohexene, bisabolene, etc. The terpene chemical components have a certain antibacterial effect, change the permeability of the cell membrane, and promote cell death; methyl salicylate destroys the lipid layer structure of the bacterial cell membrane, allowing key ions to leak from the membrane, which can not only effectively inhibit the growth of bacteria in the oral cavity, but also has an excellent preservative effect on the product itself; lemon essential oil is an aromatic oil extracted from lemon peel. It reduces bacterial adhesion by inhibiting the formation of bacterial biofilms, and reduces acid production by inhibiting the growth of Streptococcus salivarius, thereby maintaining the pH environment of the oral cavity; Melaleuca alternifolia oil has a strong inhibitory effect on fungi such as Aspergillus niger.

[0006] Furthermore, by mass percentage, the compound essential oil accounts for 0.3%-1.0% of the mouthwash, and the nonionic surfactant accounts for 1%-5% of the mouthwash.

[0007] Preferably, by mass percentage, the compound essential oil accounts for 0.37%-0.95% of the mouthwash, and the nonionic surfactant accounts for 1.7%-4.8% of the mouthwash.

[0008] Furthermore, by mass percentage, the peppermint essential oil accounts for 0.1%-0.3% of the mouthwash, the eucalyptus essential oil accounts for 0.05%-0.1% of the mouthwash, the ginger essential oil accounts for 0.05%-0.1% of the mouthwash, the methyl salicylate accounts for 0.05%-0.1% of the mouthwash, the lemon essential oil accounts for 0.1%-0.3% of the mouthwash, and the Melaleuca alternifolia oil accounts for 0.02%-0.05% of the mouthwash.

[0009] Furthermore, by mass percentage, the poloxamer 407 accounts for 0.5%-2.0% of the mouthwash, polysorbate-20 accounts for 1.0%-2.0% of the mouthwash, and PEG-60 hydrogenated castor oil accounts for 0.2%-0.8% of the mouthwash.

[0010] Furthermore, the mouthwash also includes a sweetener and a pH adjuster.

[0011] Furthermore, the mouthwash also includes glycerin, xylitol and deionized water.

[0012] Furthermore, the sweetener is one or more of saccharin sodium, aspartame, acesulfame potassium, and sucralose.

[0013] Furthermore, the pH regulator is one or more of citric acid and sodium citrate.

[0014] In a second aspect, a method for preparing a mouthwash containing the natural plant essential oil composition as described in the first aspect is provided, comprising the following steps:

[0015] S1. Add Poloxamer 407 and deionized water to an emulsifier, heat to 50-55°C, stir until completely dissolved, and then cool to 35-38°C;

[0016] S2. After fully stirring and solubilizing the compound essential oil, polysorbate 20, and PEG-60 hydrogenated castor oil to obtain a solution, the solution is added to an emulsifying pot and stirred evenly. Then, glycerin, xylitol, a sweetener, and a pH adjuster are added to the emulsifying pot to adjust the pH to 5.5-6.5. After stirring evenly, the material is discharged.

[0017] Preferably, the droplet size of the solution obtained in step S2 is less than 100 nm.

[0018] The present invention realizes introducing oil-soluble active substance in water-based mouthwash by the mode of wrapping compound essential oil synergistic enhancement by nonionic surfactant, utilizes the splendid transdermal absorbability of natural plant essential oil on the one hand, realizes antibacterial, anti-inflammatory and antioxidant effect, and stimulates skin mucosa to produce cold reflex by natural plant essential oil, brings refreshing feeling.Introduce safe and nontoxic nonionic surfactant on the other hand, impel oil-soluble substance to be evenly dispersed in water, also utilize its emulsification solubilization and foaming performance to comprehensively clean the deep throat of oral cavity, tooth root, tooth gap, tongue coating and tongue back groove line etc., the oral cavity area that brushing is difficult to touch.The mouthwash prepared by this technology has significant effect in removing oral odor, reducing oral bacteria, suppressing bacterial plaque biofilm, fresh breath, and the mouthwash prepared by the present invention is alcohol-free in addition, safer, and is applicable to the oral care of all populations. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following briefly introduces the drawings required for use in the description of the embodiments. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0020] Figure 1 The present invention is a flow chart of a method for preparing a mouthwash containing a natural plant essential oil composition;

[0021] Figure 2 This is a microscopic image of droplets of a mouthwash containing a natural plant essential oil composition according to Example 2 of the present invention;

[0022] Figure 3This is a microscopic image of droplets of a mouthwash containing a natural plant essential oil composition in Comparative Example 9 of the present invention;

[0023] Figure 4 This is a comparison of the appearance of the solution of Example 2 of the present invention and the mouthwash containing the natural plant essential oil composition of Comparative Example 9. DETAILED DESCRIPTION

[0024] The following is a clear and complete description of the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative efforts are within the scope of protection of the present invention.

[0025] It will be understood that when used in this specification and the appended claims, the terms “comprises” and “comprising” indicate the presence of described features, integers, steps, operations, elements and / or components, but do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components and / or groups thereof.

[0026] It should also be understood that the terminology used in this specification is for the purpose of describing particular embodiments only and is not intended to limit the present invention. As used in the specification and appended claims, the singular forms "a," "an," and "the" are intended to include the plural forms unless the context clearly indicates otherwise.

[0027] It should be further understood that the term "and / or" used in the present description and the appended claims refers to and includes any and all possible combinations of one or more of the associated listed items.

[0028] In order to more fully understand the technical content of the present invention, the technical solution of the present invention is further introduced and illustrated in conjunction with specific embodiments below.

[0029] The preparation method flow chart of mouthwash containing natural plant essential oil composition is as follows Figure 1 The preparation method of the mouthwash containing the natural plant essential oil composition comprises the following steps:

[0030] S1. Add poloxamer 407 and deionized water to an emulsifier, heat to 50-55°C, stir until completely dissolved, and then cool to 35-38°C;

[0031] S2. After fully stirring and solubilizing the compound essential oil, polysorbate 20, and PEG-60 hydrogenated castor oil to obtain a solution, the solution is added to an emulsifying pot and stirred evenly. Then, glycerin, xylitol, a sweetener, and a pH adjuster are added to the emulsifying pot to adjust the pH to 5.5-6.5. After stirring evenly, the material is discharged.

[0032] Preferably, the droplet size of the solution obtained in step S2 is less than 100 nm.

[0033] The preparation method of the mouthwash containing the natural plant essential oil composition described in the embodiments of the present invention and the comparative examples comprises the following steps:

[0034] S1. Add Poloxamer 407 and deionized water to an emulsifier, heat to 50°C, stir until completely dissolved, and then cool to 38°C.

[0035] S2. After fully stirring and solubilizing the compound essential oil, polysorbate 20, and PEG-60 hydrogenated castor oil to obtain a solution, the solution is added to an emulsifying pot and stirred evenly. Then, glycerin, xylitol, a sweetener, and a pH adjuster are added to the emulsifying pot, and the pH is adjusted to 6. After stirring evenly, the material is discharged.

[0036] The droplet size of the solution obtained in step S2 is less than 100 nm. The mouthwash containing the natural plant essential oil composition of Example 1 of the present invention is prepared according to the corresponding raw material components and mass percentage contents in Table 1.

[0037] Table 1 Mouthwash formula containing natural plant essential oil composition of Example 1

[0038]

[0039]

[0040] 1. Selection based on sweetener type

[0041] In addition to its significant efficacy, mouthwash should also be comfortable. Therefore, sweeteners should be introduced to mask the unpleasant taste of other ingredients, giving the product a sweet and pleasant flavor. The present invention conducts an in-depth analysis of the sweetness and safety characteristics of currently popular artificial sweeteners, as shown in Table 2:

[0042] Table 2 Comparative analysis of sweetener related properties

[0043]

[0044] From the analysis results in Table 2, it can be seen that the present invention can select at least one of the above sweeteners to adjust the sweetness, so that the taste and flavor of the mouthwash are fuller.

[0045] 2. Influence of compound essential oil types

[0046] The mouthwashes containing the natural plant essential oil compositions of Example 1 and Comparative Examples 1-6 of the present invention were prepared according to the corresponding raw material components and mass percentage contents in Table 3.

[0047] Table 3 Mouthwash formula containing natural plant essential oil composition of Example 1 and Comparative Examples 1-6

[0048]

[0049]

[0050] Wherein, compared with Example 1, Comparative Example 1 has the difference that lemon essential oil is lacking, and all other conditions are the same.Comparative Example 2 has the difference that ginger essential oil and lemon essential oil are lacking, and all other conditions are the same.Comparative Example 3 has the difference that eucalyptus essential oil, ginger essential oil and lemon essential oil are lacking, and all other conditions are the same.Comparative Example 4 has the difference that peppermint essential oil, ginger essential oil and lemon essential oil are lacking, and all other conditions are the same.Comparative Example 5 has the difference that peppermint essential oil, eucalyptus essential oil and lemon essential oil are lacking, and all other conditions are the same.Comparative Example 6 has the difference that Melaleuca alternifolia oil is lacking, and all other conditions are the same.

[0051] The mouthwashes containing the natural plant essential oil compositions of Example 1 and Comparative Examples 1-6 were subjected to taste and odor tests. The test results are shown in Table 4 below:

[0052] Table 4 Test results of mouthfeel and odor of mouthwash containing natural plant essential oil composition of Example 1 and Comparative Examples 1-6

[0053]

[0054] As can be seen from the test results in Table 4, the mouthwash containing the natural plant essential oil composition of Example 1 of the present invention has a rich taste, a pleasant smell, and a distinct sense of layering. However, the mouthwash containing the natural plant essential oil composition lacking one component of the compound essential oil (Comparative Example 1, Comparative Example 6) or multiple components (Comparative Examples 2-5) has a poor taste and smell test result, and the mouthwash containing the natural plant essential oil composition lacking multiple components is less effective than the mouthwash containing the natural plant essential oil composition lacking a single component.

[0055] The antibacterial effect of the mouthwash containing the natural plant essential oil composition of Example 1 and Comparative Example 6 was tested. The antibacterial effect test was performed according to 2.1.11.3 of the "Technical Specification for Disinfection" (2002). The test results are shown in Table 5 below:

[0056] Table 5 Antibacterial test results of mouthwash containing natural plant essential oil composition of Example 1 and Comparative Example 6

[0057]

[0058] From the test results in Table 5, it can be concluded that Melaleuca alternifolia oil has a significant improvement in the antibacterial effect. Therefore, the present invention selects peppermint essential oil, eucalyptus essential oil, ginger essential oil, methyl salicylate, lemon essential oil and Melaleuca alternifolia oil as the compound essential oil.

[0059] 3. Selection based on the type and content of nonionic surfactants

[0060] Dissolving compound essential oils in water and ensuring transparency is technically difficult. It is necessary to form a thermodynamically stable micelle solution through the solubilization of surfactants so that the droplet size in the solution is less than 100nm. Therefore, it is necessary to explore surfactants with suitable HLB values. Non-ionic surfactants have good compatibility with aqueous solutions due to their suitable HLB values. For example, PEG-N hydrogenated castor oil, polysorbates, and poloxamers are often used to solubilize oily substances. The HLB value of PEG-40 hydrogenated castor oil is 13-14, and the LD 50 5g / kg (mouse, intravenous injection), PEG-60 hydrogenated castor oil has an HLB value of 15-17, LD 50 5g / kg (mouse, intravenous injection), the HLB value of polysorbate-20 is 16.7, LD 50 37g / kg (rat, oral), the HLB value of polysorbate-80 is 15.0, LD 50 25g / kg (mouse, oral), Poloxamer 407 has an HLB value of 18-23 and is non-toxic and non-irritating. Therefore, the present invention uses nonionic surfactants: PEG-60 hydrogenated castor oil, polysorbate 20 and Poloxamer 407 to achieve the encapsulation of the compound essential oil in a transparent aqueous system.

[0061] The mouthwashes containing the natural plant essential oil compositions of Example 2 and Comparative Examples 7-9 of the present invention were prepared according to the corresponding raw material components and mass percentage contents in Table 6.

[0062] Table 6 Mouthwash formula containing natural plant essential oil composition of Example 2 and Comparative Examples 7-9

[0063]

[0064]

[0065] The appearance of the mouthwashes containing the natural plant essential oil compositions of Example 2 and Comparative Examples 7-9 was observed, and the results are shown in Table 7 below:

[0066] Table 7 Appearance of the mouthwash containing the natural plant essential oil composition of Example 2 and Comparative Examples 7-9

[0067] project Example 2 Comparative Example 7 Comparative Example 8 Comparative Example 9 Appearance status transparent transparent Slightly turbid turbid

[0068] Figure 2 This is a microscopic image of droplets of a mouthwash containing a natural plant essential oil composition according to Example 2 of the present invention; Figure 3 This is a microscopic image of droplets of the mouthwash containing a natural plant essential oil composition in Comparative Example 9 of the present invention. Figure 4 The following is a comparison of the appearance of the solution of Example 2 of the present invention and the mouthwash containing the natural plant essential oil composition of Comparative Example 9. Figure 2 and Figure 4 It can be seen that the mouthwash containing the natural plant essential oil composition in Example 2 of the present invention has no obvious oil droplets under a microscope, and the product appearance is a uniform transparent liquid, so it is judged that the droplet size in the solution is less than 100nm. Figure 3 and Figure 4 It can be seen that the mouthwash containing the natural plant essential oil composition of Comparative Example 9 of the present invention exhibits uneven droplet size distribution under a microscope, with some droplet sizes exceeding 100 nm. The product also exhibits significant whiteness. The appearance of the mouthwash containing the natural plant essential oil composition in Table 7 demonstrates that the mouthwash containing the natural plant essential oil composition of Example 2, which utilizes poloxamer 407, polysorbate 20, and PEG-60 hydrogenated castor oil as nonionic surfactants, can achieve droplet sizes in the solution of less than 100 nm, resulting in a uniform, stable, and transparent mouthwash. In contrast, the mouthwash containing the natural plant essential oil composition of Comparative Example 8, which lacks polysorbate 20 as a nonionic surfactant, exhibits a slightly turbid appearance. The mouthwash prepared in Comparative Example 9 using only Poloxamer 407 as a nonionic surfactant has a turbid appearance. This is because Poloxamer 407 has a larger HLB value, is more hydrophilic, and is less lipophilic, and therefore exhibits weaker solubilization, resulting in a larger particle size of the prepared mouthwash. However, Poloxamer 407 has good low-temperature freeze-thaw resistance, which helps the mouthwash maintain good fluidity at low temperatures, and therefore plays an important role in the composition.

[0069] The mouthwashes containing the natural plant essential oil compositions of Example 2 and Comparative Examples 10-13 of the present invention were prepared according to the corresponding raw material components and mass percentage contents in Table 8.

[0070] Table 8 Mouthwash formula containing natural plant essential oil composition of Example 2 and Comparative Examples 10-13

[0071]

[0072] The appearance of the mouthwashes containing the natural plant essential oil compositions of Example 2 and Comparative Examples 10-13 was observed, and the results are shown in Table 9 below:

[0073] Table 9 Appearance of the mouthwash containing the natural plant essential oil composition of Example 2 and Comparative Examples 10-13

[0074] project Example 2 Comparative Example 10 Comparative Example 11 Comparative Example 12 Comparative Example 13 Appearance status transparent Slightly turbid Slightly turbid Slightly turbid Slightly turbid

[0075] The results of the appearance of mouthwashes containing natural plant essential oil compositions in Table 9 show that the mouthwash containing a natural plant essential oil composition prepared without PEG-60 hydrogenated castor oil as a nonionic surfactant (Comparative Example 10) or the mouthwash containing a natural plant essential oil composition prepared in which polysorbate 20, poloxamer 407, and PEG-60 hydrogenated castor oil in the nonionic surfactant are not within the scope of the present invention all appear slightly turbid.

[0076] The mouthwash containing the natural plant essential oil composition prepared based on Comparative Example 7 in the absence of PEG-60 hydrogenated castor oil as a nonionic surfactant and the mouthwash containing the natural plant essential oil composition of Example 2 both have a transparent appearance. The mouthwash containing the natural plant essential oil composition of Example 2 and Comparative Example 7 was subjected to a centrifugation test. The centrifugation test conditions were 2000 r / min for 30 minutes, and then the appearance of the mouthwash was observed. The test results are shown in Figure 10 below:

[0077] Table 10 Centrifugation test results of mouthwash containing natural plant essential oil composition of Example 2 and Comparative Example 7

[0078] project Example 2 Comparative Example 7 Centrifuge test Uniform without stratification or precipitation Upper floating oil

[0079] As can be seen from the test results in Table 10, the mouthwash containing the natural plant essential oil composition prepared in Comparative Example 7, which lacks PEG-60 hydrogenated castor oil as a nonionic surfactant, failed the centrifugation test.

[0080] 4. High and low stability test of mouthwash containing natural plant essential oil composition

[0081] The mouthwash containing the natural plant essential oil composition of Example 2 was subjected to high and low stability tests, and the appearance, odor and pH of the mouthwash were tested respectively. The high and low temperature stability test conditions and results of the mouthwash containing the natural plant essential oil composition of Example 2 are shown in Table 11 below:

[0082] Table 11 Test conditions and results of high and low temperature stability of mouthwash containing natural plant essential oil composition in Example 2

[0083]

[0084] The test results in Table 11 show that after 12 weeks of high and low temperature stability testing, the appearance, odor, and pH of the mouthwash containing the natural plant essential oil composition of Example 2 were normal. This shows that the mouthwash containing the natural plant essential oil composition of the present invention has good stability.

[0085] 5. Antiseptic Performance Test of Mouthwash Containing Natural Plant Essential Oil Composition

[0086] The antiseptic performance of the mouthwash containing the natural plant essential oil composition of Example 2 was tested, wherein the tested microorganisms mainly included bacteria, yeast, and Aspergillus niger.

[0087] The bacterial suspension of the test sample shall be prepared in accordance with 2.1.1.2.3 of the Technical Specification for Disinfection (2002), and the fungal suspension shall be prepared in accordance with 2.1.1.9.3 of the Technical Specification for Disinfection (2002). The following test methods shall be used for sample testing:

[0088] After weighing the above samples, add 0.3 mL of mixed bacterial suspension and 0.3 mL of mixed fungal suspension, respectively. Store the samples at room temperature (22.5±2.5°C) and test the colony count in the samples on days 7, 14, 21, and 28 after inoculation. Specifically, on days 7, 14, 21, and 28 after inoculation, select 1 mL of each of the inoculated bacterial and fungal samples, add 9 mL of PBS, and mix thoroughly. Aspirate the sample and count the viable colonies. For samples with a log reduction of 3 or greater on day 7, perform a second bacterial addition test on day 14, following the above method.

[0089] The method for determining the antiseptic performance test results is as follows: a mouthwash containing a natural plant essential oil composition is inoculated with bacteria once and tested for colony counts at 0 hours and 7 days. If the decrease in the count is greater than 2, the mouthwash passes the first stage of the test. Then, a mouthwash containing a natural plant essential oil composition is inoculated with bacteria twice and tested for colony counts at 0 hours, 7 days, and 14 days. If the count is greater than 3, the mouthwash passes the second stage of the test. The antiseptic performance test conditions and results of the mouthwash containing a natural plant essential oil composition in Example 2 are shown in Tables 12 to 14 below:

[0090] Table 12 Test conditions and results of the antiseptic properties of mouthwash containing natural plant essential oil composition in Example 2

[0091]

[0092]

[0093] Table 13 Test conditions and results of the antiseptic properties of mouthwash containing natural plant essential oil composition in Example 2

[0094]

[0095] Table 14 Example 2 Antiseptic performance test conditions and results of mouthwash containing natural plant essential oil composition

[0096]

[0097] The test results in Tables 12-14 show that the mouthwash containing the natural plant essential oil composition in Example 2 has an excellent inhibitory effect on common microorganisms, can effectively avoid microbial contamination caused by daily use, and therefore has good antiseptic properties.

[0098] VI. Irritation Test of Mouthwash Containing Natural Plant Essential Oil Composition

[0099] The mouthwash containing the natural plant essential oil composition of Example 2 was subjected to an irritation test (eye irritation test).

[0100] 1. Test Method

[0101] The toxicity and irritation of the product were assessed using the standard SN / T 2329-2009, "Chicken Embryo Chorioallantoic Membrane Test for Eye Irritation / Corrosion of Cosmetics." SPF embryos should be used, unfertilized within 7 days, and incubation conditions strictly adhered to the standard. At least six embryos were tested in parallel per group, with one embryo in each control group, depending on the experiment. The experimental liquid was dripped directly onto the chorioallantoic membrane (CAM) and the CAM reaction was observed. The time to onset of each toxic effect, including bleeding, coagulation, and vasolysis, was recorded within 5 minutes.

[0102] This study used the reaction time method (IS) to conduct the experiment. The stimulus score (IS) was calculated using formula (1), and the result was rounded to two decimal places:

[0103]

[0104] Where:

[0105] secH (bleeding time) - the average time to the onset of bleeding observed on the CAM membrane, in seconds (s);

[0106] secL (vascular lysis time) - the average time to the onset of vascular lysis observed on the CAM membrane, in seconds (s);

[0107] secC (clotting time) - the average time in seconds to observe the onset of clotting on the CAM membrane.

[0108] Based on the calculated IS values, classify the eye irritation of the test substance according to Table 15.

[0109] Table 15 Irritation score and irritation classification

[0110] Stimulus score Irritation classification IS<1 Non-irritating 1≤IS<5 Mild irritation 5≤IS<9 Moderate irritation IS≥10 Strong irritant / corrosive

[0111] 2. Experimental Results

[0112] The irritation test results of the mouthwash containing the natural plant essential oil composition in Example 2 are shown in Table 16 below:

[0113] Table 16 Example 2 Mouthwash irritation test results containing natural plant essential oil composition

[0114]

[0115]

[0116] From the test results in Table 16, it can be seen that the IS values ​​of the six chicken embryos are all less than 1. According to the scoring criteria, the mouthwash containing the natural plant essential oil composition in Example 2 is non-irritating.

[0117] 7. Cleansing Performance Test of Mouthwash Containing Natural Plant Essential Oil Composition

[0118] The cleaning performance test was conducted on the mouthwashes containing the natural plant essential oil compositions of Example 2 and Comparative Examples 7-9. The cleaning performance test requirements are as follows:

[0119] 1. Crowd selection

[0120] There were 10 test subjects who did not brush their teeth after getting up in the morning, aged 25 to 50 years old; 5 test subjects who smoked; 5 test subjects who drank alcohol; and 20 test subjects who ate lunch, aged 25 to 50 years old.

[0121] 2. Testing requirements

[0122] Lunch consists of common foods with strong flavors, including: hot pot, garlic, durian, and stinky tofu.

[0123] 3. Test Method

[0124] The test method will be divided into four categories of people: A, B, C, and D for evaluation.

[0125] A. For those who did not brush their teeth after getting up in the morning: rinse with 10g of mouthwash for 30 seconds and spit it out. After half an hour, assess whether the mouth is fresh (do not eat or drink anything during this period).

[0126] B. Smoking tester: After smoking, use 10g of mouthwash, rinse your mouth for 30 seconds and spit it out. After half an hour, evaluate whether the mouth is fresh (no food should be consumed during this period).

[0127] C. Drinking tester: After drinking, use 10g of mouthwash, rinse your mouth for 30 seconds and spit it out. After half an hour, evaluate whether the mouth is fresh (no food should be consumed during this period).

[0128] D. Lunch test subjects: The test subjects' lunch consisted of hot pot, garlic, durian, and stinky tofu. After eating, they sat quietly for half an hour, rinsed their mouths with 10g of mouthwash for 30 seconds, and spit it out. After half an hour, the freshness of the mouth was assessed (no food was allowed during this period).

[0129] 4. Scoring requirements

[0130] The total score is 10 points, 1 point is the worst, no cleaning effect, 5 points are average, no significant cleaning effect, 10 points are the full score, with significant cleaning effect, and an average score of ≥ 7 points is considered to have cleaning performance.

[0131] The cleaning performance tests of the mouthwashes containing natural plant essential oil compositions of Example 2 and Comparative Examples 16-18 are shown in Table 17 below:

[0132] Table 17 Test results of cleaning performance of mouthwash containing natural plant essential oil composition in Example 2 and Comparative Examples 7-9

[0133]

[0134] The test results in Table 17 demonstrate that the mouthwash containing the natural plant essential oil composition of Example 2 of the present invention exhibits significant cleaning performance. However, mouthwashes containing the natural plant essential oil composition lacking a single component (Comparative Examples 7 and 8) or multiple components (Comparative Example 9) of the nonionic surfactant exhibited poor cleaning performance.

[0135] In summary, the mouthwash containing the natural plant essential oil composition of the present invention has good antiseptic properties, the irritation performance test results are non-irritating, and the cleaning performance test results show significant cleaning performance. Therefore, the mouthwash containing the natural plant essential oil composition of the present invention not only solves the solubility problem of natural plant essential oils in aqueous products, but is also mild and non-irritating, and has significant efficacy.

[0136] The above description is merely a specific embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any person skilled in the art can easily conceive of various equivalent modifications or substitutions within the technical scope disclosed in the present invention, and such modifications or substitutions are intended to be within the scope of protection of the present invention. Therefore, the scope of protection of the present invention shall be subject to the scope of protection of the claims.

Claims

1. A mouthwash containing a natural plant essential oil composition, characterized in that: Contains a natural plant essential oil composition, wherein the natural plant essential oil composition is composed of a compound essential oil and a non-ionic surfactant, wherein the compound essential oil is composed of peppermint essential oil, eucalyptus essential oil, ginger essential oil, methyl salicylate, lemon essential oil and Melaleuca alternifolia oil; and the non-ionic surfactant is composed of poloxamer 407, polysorbate 20 and PEG-60 hydrogenated castor oil. By mass percentage, the peppermint essential oil accounts for 0.1%-0.3% of the mouthwash, the eucalyptus essential oil accounts for 0.05%-0.1% of the mouthwash, the ginger essential oil accounts for 0.05%-0.1% of the mouthwash, the methyl salicylate accounts for 0.05%-0.1% of the mouthwash, the lemon essential oil accounts for 0.1%-0.3% of the mouthwash, and the Melaleuca alternifolia oil accounts for 0.02%-0.05% of the mouthwash; By mass percentage, the poloxamer 407 accounts for 0.5%-2.0% of the mouthwash, polysorbate-20 accounts for 1.0%-2.0% of the mouthwash, and PEG-60 hydrogenated castor oil accounts for 0.2%-0.8% of the mouthwash.

2. The mouthwash containing natural plant essential oil composition as claimed in claim 1, wherein The mouthwash also includes a sweetener and a pH regulator.

3. The mouthwash containing natural plant essential oil composition as claimed in claim 2, wherein The mouthwash also includes glycerin, xylitol and deionized water.

4. The mouthwash containing natural plant essential oil composition as claimed in claim 3, wherein The sweetener is one or more of saccharin sodium, aspartame, acesulfame potassium, and sucralose.

5. The mouthwash containing natural plant essential oil composition as claimed in claim 4, wherein The pH regulator is one or more of citric acid and sodium citrate.

6. A method for preparing a mouthwash containing a natural plant essential oil composition according to any one of claims 3 to 5, characterized in that: The following steps are involved: S1. Add Poloxamer 407 and deionized water to an emulsifier, heat to 50-55°C, stir until completely dissolved, and then cool to 35-38°C; S2. After fully stirring and solubilizing the compound essential oil, polysorbate 20, and PEG-60 hydrogenated castor oil to obtain a solution, the solution is added to an emulsifying pot and stirred evenly. Then, glycerin, xylitol, a sweetener, and a pH adjuster are added to the emulsifying pot to adjust the pH to 5.5-6.

5. After stirring evenly, the material is discharged.

7. The method for preparing a mouthwash containing a natural plant essential oil composition as claimed in claim 6, wherein: The droplet size of the solution obtained in step S2 is less than 100 nm.

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