A probiotic fermented Chinese medicine product for treating swine Escherichia coli diarrhea and its preparation method and application

Abstract

The present invention discloses a probiotic fermented Chinese medicine product for treating Escherichia coli diarrhea from pigs, and its preparation method and application, which belong to the field of Chinese veterinary medicine. The probiotic fermented Chinese medicine product is prepared by fermenting a Chinese medicine compound mixture with probiotics as the fermentation substrate; wherein the Chinese medicine compound mixture includes a Chinese medicine composition and soybean meal, and the Chinese medicine composition includes: Pueraria root, Scutellaria baicalensis, Coptis chinensis, Codonopsis pilosula, Poria cocos, stir-fried Atractylodes macrocephala, charred Terminalia chebula, Alisma orientalis, dried ginger and licorice; in the Chinese medicine compound mixture, the mass percentage of the Chinese medicine composition is ≥80%; the probiotics include lactobacillus, yeast, Bacillus subtilis and Bacillus licheniformis. It has been found through experiments that the probiotic fermented Chinese medicine product can significantly reduce liver and spleen enlargement caused by Escherichia coli, and can control mouse inflammation by reducing the levels of pro-inflammatory cytokines and increasing the levels of anti-inflammatory cytokines, and improve the immune function of mice, thereby effectively treating diarrhea caused by Escherichia coli.

Description

Technical Field

[0001] The invention relates to the technical field of traditional Chinese veterinary medicines, and in particular to a probiotic fermented traditional Chinese medicine product for treating swine Escherichia coli diarrhea, and a preparation method and application thereof. Background Art

[0002] Swine edema disease, diarrhea in newborn piglets and diarrhea in weaned piglets caused by Escherichia coli cause huge economic losses to the pig farming industry, mainly affecting newborn piglets and piglets in the first two weeks after weaning, characterized by sudden death or diarrhea, dehydration and growth retardation in surviving piglets. The traditional method to prevent Escherichia coli disease in pigs is to supplement antibiotics in piglet diets, but long-term overuse of antibiotics has led to increased bacterial resistance and antibiotic residues in animal products, which seriously threatens human health; it also causes intestinal flora dysbiosis and superinfection of piglets, so my country has banned the addition of antibiotics to livestock diets. In this context, it has become a top priority to find alternatives to antibiotics to protect the health of piglets during the critical weaning period.

[0003] With the prohibition of antibiotics in animal husbandry and the demand for alternatives, Chinese herbal medicine additives have become a popular choice for feed additives due to their non-drug resistance and diverse probiotic functions. However, natural Chinese herbal medicines currently have disadvantages such as large dosage, difficulty in dissolving active ingredients, and poor utilization of Chinese herbal medicine resources, which affect their competitiveness in the veterinary drug and feed additive market. Therefore, the effective development of Chinese herbal medicines through biocatalysis technology has become a must. Summary of the invention

[0004] The purpose of the present invention is to provide a probiotic fermented Chinese medicine product for treating porcine Escherichia coli diarrhea and its preparation method and application, so as to solve the problems existing in the above-mentioned prior art. The probiotic fermented Chinese medicine product at this dose can control inflammation in mice and improve the immune function of mice by reducing the levels of pro-inflammatory cytokines (IL-1β, IL-6, IFN-γ, TNF-α) and increasing the levels of anti-inflammatory cytokines (IL-4, IL-10), thereby effectively treating diarrhea caused by Escherichia coli.

[0005] To achieve the above object, the present invention provides the following solutions:

[0006] The present invention provides a probiotic fermented Chinese medicine product for treating porcine Escherichia coli diarrhea, wherein the probiotic fermented Chinese medicine product is prepared by fermenting a Chinese medicine compound mixture as a fermentation substrate using probiotics;

[0007] The Chinese medicine compound mixture comprises a Chinese medicine composition and soybean meal, wherein the Chinese medicine composition comprises the following Chinese medicine raw materials in parts by weight: 10-25 parts of Pueraria root, 7-15 parts of Scutellaria baicalensis, 7-15 parts of Coptis chinensis, 10-25 parts of Codonopsis pilosula, 10-25 parts of Poria cocos, 10-25 parts of stir-fried Atractylodes macrocephala, 10-25 parts of charred Terminalia chebula, 6-17 parts of Alisma orientalis, 6-17 parts of dried ginger and 6-17 parts of Licorice; in the Chinese medicine compound mixture, the mass percentage of the Chinese medicine composition is ≥80%;

[0008] The probiotics include lactobacillus, yeast, bacillus subtilis and bacillus licheniformis.

[0009] Preferably, the Chinese medicine composition comprises the following Chinese medicine raw materials in parts by weight: 15 parts of Pueraria root, 9 parts of Scutellaria baicalensis, 9 parts of Coptis chinensis, 15 parts of Codonopsis pilosula, 15 parts of Poria cocos, 15 parts of stir-fried Atractylodes macrocephala, 15 parts of charred Terminalia chebula, 10 parts of Alisma orientalis, 10 parts of dried ginger and 10 parts of Licorice.

[0010] Preferably, the lactobacillus is Lactobacillus fermentum, with a preservation number of CGMCC No.4648; the yeast is Saccharomyces cerevisiae, with a preservation number of CGMCC No.16952; the Bacillus subtilis has a preservation number of CGMCC No.3440; and the Bacillus licheniformis has a preservation number of CGMCC No.7388.

[0011] Preferably, the viable counts of the fermented Lactobacillus, Saccharomyces cerevisiae, Bacillus subtilis and Bacillus licheniformis are not less than 1×10 9 CFU / mL.

[0012] The present invention also provides a method for preparing the probiotic fermented Chinese medicine product for treating swine Escherichia coli diarrhea, comprising the following steps:

[0013] (1) According to the dosage of the formula, kudzu root, scutellaria baicalensis, coptis root, codonopsis pilosula, poria, stir-fried atractylodes macrocephala, charred terminalia chebula, oriental water chestnut, dried ginger and liquorice are crushed and sieved, and then soybean meal is added and mixed evenly to obtain a traditional Chinese medicine compound mixture;

[0014] Mixing and culturing a probiotic mixed bacterial liquid containing lactobacillus, yeast, Bacillus subtilis and Bacillus licheniformis with the traditional Chinese medicine compound mixture to obtain a fermentation product of the compound traditional Chinese medicine and the probiotic mixture;

[0015] (3) freeze-drying and crushing the fermented product of the compound Chinese medicine and the probiotic mixture to obtain a probiotic fermented Chinese medicine product for treating porcine Escherichia coli diarrhea.

[0016] Preferably, in (1), sieving is performed using a 60-mesh sieve.

[0017] Preferably, in (1), 5-80 mL of the probiotic mixed bacterial solution is added to every 1 kg of the traditional Chinese medicine compound mixture, and 320-395 mL of distilled water is added at the same time, and the mixture is mixed evenly and then mixed and cultured.

[0018] Preferably, the probiotic mixed bacterial liquid is obtained by fermenting lactobacillus, yeast, bacillus subtilis and bacillus licheniformis separately, and then mixing the lactobacillus fermentation liquid, yeast fermentation liquid, bacillus subtilis fermentation liquid and bacillus licheniformis fermentation liquid in a volume ratio of 1:1:1:1.

[0019] Preferably, the mixed culture conditions are: fermentation at 35° C. for 48 hours.

[0020] The present invention also provides the use of the probiotic fermented Chinese medicine product in preparing medicine or feed additive for treating diarrhea caused by Escherichia coli.

[0021] The present invention discloses the following technical effects:

[0022] The probiotic fermented Chinese medicinal product for treating swine Escherichia coli diarrhea prepared by the present invention adopts freeze-drying technology, can retain the probiotics in the fermentation process to the maximum extent, and can achieve better probiotic effects by being mixed into animal feed.

[0023] The present invention verifies through animal mouse experiments that the diarrhea rate and mortality rate of mice in the high-dose group of probiotic fermented Chinese medicine products for treating porcine Escherichia coli diarrhea are lower than those in the florfenicol group, and are much lower than those in the Escherichia coli model group. This shows that the probiotic fermented Chinese medicine products provided by the present invention have a good therapeutic effect on diarrhea caused by Escherichia coli. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0025] Figure 1 The figures show the effects of different groups of experiments on the functions of various organs. Different lowercase letters in the figure indicate significant differences for the same organ index, while the same lowercase letters indicate insignificant differences. DETAILED DESCRIPTION

[0026] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as limiting the present invention, but should be understood as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0027] It should be understood that the terms described in the present invention are only for describing a particular embodiment and are not intended to limit the present invention. In addition, for the numerical range in the present invention, it should be understood that each intermediate value between the upper and lower limits of the scope is also specifically disclosed. The intermediate value in any stated value or stated range, and each smaller range between any other stated value or intermediate value in the described range is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded in the scope.

[0028] Unless otherwise indicated, all technical and scientific terms used herein have the same meanings as those generally understood by those skilled in the art. Although the present invention describes only preferred methods and materials, any methods and materials similar or equivalent to those described herein may also be used in the implementation or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials associated with the documents. In the event of a conflict with any incorporated document, the content of this specification shall prevail.

[0029] It will be apparent to those skilled in the art that various modifications and variations may be made to the specific embodiments of the present invention description without departing from the scope or spirit of the present invention. Other embodiments derived from the present invention description will be apparent to those skilled in the art. The present invention description and examples are exemplary only.

[0030] The words “include,” “including,” “have,” “contain,” etc. used in this document are open-ended terms, meaning including but not limited to.

[0031] Used in the following examples, lactobacillus fermentum (preservation number is CGMCC No.4648), saccharomyces cerevisiae (preservation number is CGMCC No.16952), bacillus subtilis (preservation number is CGMCC No.3440), and bacillus licheniformis (preservation number is CGMCC No.7388) are all purchased from the General Microbiological Center of China National Committee for the Collection of Microorganisms.

[0032] The following examples prepare a mixed bacterial solution of Lactobacillus fermentum, Saccharomyces cerevisiae, Bacillus subtilis and Bacillus licheniformis, and the method is as follows:

[0033] (1) inoculating 3% of Lactobacillus fermentum into MRS medium (purchased from Guangdong Huankai Microbiological Technology Co., Ltd.), and fermenting at 35° C. for 36 h to obtain Lactobacillus fermentum fermentation liquid;

[0034] The brewer's yeast was inoculated into YPD medium at 5%, and fermented at 35°C for 48 hours to obtain the brewer's yeast fermentation liquid; the YPD medium was: 10g yeast extract, 20g peptone and 20g glucose, and the volume was fixed to 1L;

[0035] Inoculate the fermentation medium with Bacillus subtilis at 3%, and ferment and culture at 35°C for 36 hours to obtain Bacillus subtilis fermentation liquid; the fermentation medium is: 20g glucose, 15g peptone, 5g sodium chloride and 20g beef extract, and the volume is fixed to 1L;

[0036] Inoculate the fermentation medium with 4% Bacillus licheniformis, and ferment and culture at 37°C for 36 hours to obtain Bacillus licheniformis fermentation liquid; the fermentation medium is 10g of peptone, 5g of yeast extract and 10g of sodium chloride, and the volume is fixed to 1L;

[0037] (2) The fermentation liquid of Lactobacillus fermentation, the fermentation liquid of Saccharomyces cerevisiae, the fermentation liquid of Bacillus subtilis and the fermentation liquid of Bacillus licheniformis prepared above are uniformly mixed in a volume ratio of 1:1:1:1 to obtain a mixed bacterial liquid of Lactobacillus fermentation, Saccharomyces cerevisiae, Bacillus subtilis and Bacillus licheniformis.

[0038] Example 1

[0039] The preparation method of a probiotic fermented Chinese medicine product for treating swine Escherichia coli diarrhea comprises the following steps:

[0040] Step A: 15 g of Pueraria root, 9 g of Scutellaria baicalensis, 9 g of Coptis chinensis, 15 g of Codonopsis pilosula, 15 g of Poria, 15 g of stir-fried Atractylodes macrocephala, 15 g of charred Terminalia chebula, 10 g of Orientalis, 10 g of dried ginger, and 10 g of Licorice are mixed by weight, then crushed and passed through a 60-mesh sieve to obtain a Chinese medicine composition, and then 20% soybean meal is added (i.e., 20 g of soybean meal is added to every 80 g of the Chinese medicine composition), and mixed in a mixer for 15 minutes to obtain a Chinese medicine compound mixture;

[0041] Step B: Add 30 mL of fermented lactic acid bacteria (viable count 1×10 9 CFU / mL), Saccharomyces cerevisiae (viable count 1×10 9 CFU / mL), Bacillus subtilis (viable count 1×10 9 CFU / mL), Bacillus licheniformis (viable count 1×10 9 CFU / mL), and 370 mL of sterile distilled water was added, and the mixture was stirred and mixed to obtain a mixture of the compound Chinese medicine and probiotics;

[0042] Step C: placing the mixture of the compound Chinese medicine and the probiotics obtained in step B in a fermentation bag with a one-way valve, and fermenting at 35° C. for 48 hours to obtain a fermentation product of the mixture of the compound Chinese medicine and the probiotics;

[0043] Step D: The fermented product of the mixture of the compound Chinese medicine and probiotics obtained in step C is taken out, placed in a freeze dryer for freeze drying, then crushed and bagged to obtain a probiotic fermented Chinese medicine product for treating porcine Escherichia coli diarrhea.

[0044] Step E: Weigh an appropriate amount of the probiotic fermented Chinese medicine product for treating swine Escherichia coli diarrhea obtained in step D, dissolve it in sterile distilled water to make the concentration of the fermented Chinese medicine reach 31.25 mg / mL, and obtain a mixed solution 1 of the fermented Chinese medicine product and sterile distilled water.

[0045] Example 2

[0046] The preparation method of a probiotic fermented Chinese medicine product for treating swine Escherichia coli diarrhea comprises the following steps:

[0047] Step A: 15 g of Pueraria root, 9 g of Scutellaria baicalensis, 9 g of Coptis chinensis, 15 g of Codonopsis pilosula, 15 g of Poria, 15 g of stir-fried Atractylodes macrocephala, 15 g of charred Terminalia chebula, 10 g of Orientalis, 10 g of dried ginger, and 10 g of Licorice are mixed by weight, then crushed and passed through a 60-mesh sieve to obtain a Chinese medicine composition, and then 20% soybean meal is added (i.e., 20 g of soybean meal is added to every 80 g of the Chinese medicine composition), and mixed in a mixer for 15 minutes to obtain a Chinese medicine compound mixture;

[0048] Step B: Add 30 mL of fermented lactic acid bacteria (viable count 1×10 9 CFU / mL), Saccharomyces cerevisiae (viable count 1×10 9 CFU / mL), Bacillus subtilis (viable count 1×10 9 CFU / mL), Bacillus licheniformis (viable count 1×10 9 CFU / mL), and 370 mL of sterile distilled water was added, and the mixture was stirred and mixed to obtain a mixture of the compound Chinese medicine and probiotics;

[0049] Step C: placing the mixture of the compound Chinese medicine and the probiotics obtained in step B in a fermentation bag with a one-way valve, and fermenting at 35° C. for 48 hours to obtain a fermentation product of the mixture of the compound Chinese medicine and the probiotics;

[0050] Step D: The fermented product of the mixture of the compound Chinese medicine and probiotics obtained in step C is taken out, placed in a freeze dryer for freeze drying, then crushed and bagged to obtain a probiotic fermented Chinese medicine product for treating porcine Escherichia coli diarrhea.

[0051] Step E: Weigh an appropriate amount of the probiotic fermented Chinese medicine product for treating swine Escherichia coli diarrhea obtained in step D, dissolve it in sterile distilled water to make the concentration of the fermented Chinese medicine reach 62.5 mg / mL, and obtain a mixed solution 2 of the fermented Chinese medicine product and sterile distilled water.

[0052] Example 3

[0053] The preparation method of a probiotic fermented Chinese medicine product for treating swine Escherichia coli diarrhea comprises the following steps:

[0054] Step A: 15 g of Pueraria root, 9 g of Scutellaria baicalensis, 9 g of Coptis chinensis, 15 g of Codonopsis pilosula, 15 g of Poria, 15 g of stir-fried Atractylodes macrocephala, 15 g of charred Terminalia chebula, 10 g of Orientalis, 10 g of dried ginger, and 10 g of Licorice are mixed by weight, then crushed and passed through a 60-mesh sieve to obtain a Chinese medicine composition, and then 20% soybean meal is added (i.e., 20 g of soybean meal is added to every 80 g of the Chinese medicine composition), and mixed in a mixer for 15 minutes to obtain a Chinese medicine compound mixture;

[0055] Step B: Add 30 mL of fermented lactic acid bacteria (viable count 1×109 CFU / mL) and 30 mL of brewer's yeast (viable count 1×10 9 CFU / mL), Bacillus subtilis (viable count 1×10 9 CFU / mL), Bacillus licheniformis (viable count 1×10 9 CFU / mL), and 370 mL of sterile distilled water was added, and the mixture was stirred and mixed to obtain a mixture of the compound Chinese medicine and probiotics;

[0056] Step C: placing the mixture of the compound Chinese medicine and the probiotics obtained in step B in a fermentation bag with a one-way valve, and fermenting at 35° C. for 48 hours to obtain a fermentation product of the mixture of the compound Chinese medicine and the probiotics;

[0057] Step D: The fermented product of the mixture of the compound Chinese medicine and probiotics obtained in step C is taken out, placed in a freeze dryer for freeze drying, then crushed and bagged to obtain a probiotic fermented Chinese medicine product for treating porcine Escherichia coli diarrhea.

[0058] Step E: Weigh an appropriate amount of the probiotic fermented Chinese medicine product for treating swine Escherichia coli diarrhea obtained in step D, dissolve it in sterile distilled water to make the concentration of the fermented Chinese medicine reach 125 mg / mL, and obtain a mixed solution 3 of the fermented Chinese medicine product and sterile distilled water.

[0059] Comparative experiment

[0060] Using florfenicol as a positive control drug, the mixed solution of the probiotic fermented Chinese medicine product for treating porcine Escherichia coli diarrhea prepared in Examples 1, 2 and 3 and sterile distilled water was observed for the treatment of porcine Escherichia coli-induced diarrhea in mice.

[0061] Methods: A number of female Kunming mice aged 6 to 8 weeks (more than 56) were used. 8 mice were reserved for intraperitoneal injection of 200 μL saline as the blank group; the rest were intraperitoneally injected with 1×10 8CFU / mL of porcine Escherichia coli 200 μL, after the onset of diarrhea symptoms (about 6h after the attack), 48 diarrhea mice were selected and divided into model group, florfenicol group, unfermented Chinese medicine group (62.5mg / mL), Example 3 group (125mg / mL), Example 2 group (62.5mg / mL) and Example 1 group (31.25mg / mL), 8 in each group. The corresponding medium-dose unfermented Chinese medicine solution, 3 doses of fermented Chinese medicine solution and 1% florfenicol solution were prepared with sterile distilled water. The mice in the medium-dose group of unfermented Chinese medicine were gavaged with 1mL of Chinese medicine every day, the mice in the fermented Chinese medicine group were gavaged with 1mL of fermented Chinese medicine every day, the mice in the blank group and the model group were gavaged with 1mL of distilled water, and the mice in the florfenicol group were gavaged with 1% florfenicol 1mL, and the continuous gavage was continued for 5 days, and the last gavage was fasted for 12h, and the mice were weighed on an empty stomach, and the mice in each group were killed and sampled.

[0062] Blood was collected by removing the eyeballs and aseptically collecting whole blood in a 2 mL centrifuge tube. The mice were quickly killed by short neck when they stopped bleeding. The whole blood was placed in a 4 °C refrigerator for 2 h, centrifuged at 3000 r / min for 10 min, and the upper serum was collected in a 1.5 mL centrifuge tube and stored at -20 °C for later use.

[0063] The ELISA method was used to detect the IL-1β, IL-4, IL-6, TNF-α, IL-10, and IFN-γ indicators in the serum of each group of mice. The specific method was carried out strictly according to the instructions.

[0064] After the mice were killed, they were immediately dissected and the heart, liver, spleen, lungs and kidneys were collected and weighed, and the organ index (mg / g) was calculated.

[0065] The test results are as follows:

[0066] Table 1 Diarrhea and death of mice in each group

[0067]

[0068] As can be seen from Table 1, except for the blank group, the mortality rate of Example 3 group was the lowest, with only one mouse dying, and no mice had diarrhea after administration on the third day; the results showed that the mixed solution 3 of the fermented Chinese medicine product and sterile distilled water had a good therapeutic effect on Escherichia coli diarrhea;

[0069] Depend on Figure 1 It can be seen that: (1) the liver index of the blank group, the Chinese medicine group and the Example 3 group was significantly lower than that of the model group; (2) the spleen index of the blank group and the Example 3 group was significantly lower than that of the model group. The results show that the mixed solution 3 of the fermented Chinese medicine product and sterile distilled water can reduce the enlargement of the liver and spleen caused by Escherichia coli;

[0070] Table 2 Cytokine content in serum of mice in each group (pg / mL)

[0071]

[0072] Note: Different lowercase letters in the same row indicate significant differences, while the same lowercase letters indicate insignificant differences.

[0073] It can be seen from Table 2 that: (1) the IL-1β content in the serum of the model group was significantly higher than that of the other groups (229.00 pg / mL), the lowest in the blank group (66.95 pg / mL), the Example 3 group and the antibiotic group were closest to the blank group and the difference was not significant (P>0.05); (2) the IL-4 content was the highest in the blank group (83.87 pg / mL), which was significantly higher than that of the model group (50.39 pg / mL, P<0.05), the Example 3 group and the antibiotic group were closest to the blank group and the difference was not significant (P>0.05); (3) the Example 2 group, the Example 3 group, The content of IL-6 in the serum of the antibiotic group was significantly lower than that of the model group (P<0.05), and was not significantly different from that of the blank group (P>0.05); (4) the content of IL-10 in Example 2 group, Example 3 group, and antibiotic group was significantly higher than that of the model group (P<0.05), and was also higher than that of the blank group, but the difference was not significant (P>0.05); (5) the content of TNF-α in the blank group was significantly lower than that of the other groups (P<0.05); (6) the content of IFN-γ in Example 2 group, Example 3 group, and antibiotic group was significantly lower than that of the model group (P<0.05), and was higher than that of the blank group, but the difference was not significant (P>0.05);

[0074] The above results indicate that the mixed solution 3 of the fermented Chinese medicine product and sterile distilled water can control inflammation in mice and improve the immune function of mice by reducing the levels of pro-inflammatory cytokines (IL-1β, IL-6, IFN-γ, TNF-α) and increasing the levels of anti-inflammatory cytokines (IL-4, IL-10), thereby effectively treating diarrhea caused by Escherichia coli.

[0075] The probiotic fermented Chinese medicine provided by the present invention is based on the traditional Chinese medicine fermentation process. The content of active ingredients can be increased by microbial fermentation of Chinese medicine. The extracellular enzymes such as cellulase and pectinase produced by the microorganisms can cause the Chinese medicine cells to rupture and expose the active ingredients, and the effective macromolecular substances are degraded into small molecules that are more easily absorbed by the human body or animals. At the same time, it has the advantages of enhancing the efficacy, expanding the indications of Chinese medicine, reducing the toxicity of Chinese medicine, regulating intestinal flora, improving the taste and improving the utilization of Chinese medicine resources. It can be seen from the above-mentioned animal experimental results that the present invention can be added to the Chinese medicine composition after the four microorganisms are compounded, and the Chinese medicine composition and soybean meal can be used as fermentation substrates to convert them into small molecules that are easy to be absorbed by animals, and then the levels of pro-inflammatory cytokines and anti-inflammatory cytokines are reduced by the effective components produced to achieve control of animal inflammation, improve animal immunity, and effectively treat diarrhea caused by Bacillus cereus.

[0076] The embodiments described above are only descriptions of the preferred modes of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should all fall within the protection scope determined by the claims of the present invention.

Claims

1. A probiotic fermented Chinese medicine product for treating porcine Escherichia coli diarrhea, characterized in that: The probiotic fermented Chinese medicine product is prepared by fermenting a Chinese medicine compound mixture with probiotics as a fermentation substrate; The Chinese medicine compound mixture is composed of a Chinese medicine composition and soybean meal, and the Chinese medicine composition is made of the following Chinese medicine raw materials in parts by weight: 10-25 parts of Pueraria root, 7-15 parts of Scutellaria baicalensis, 7-15 parts of Coptis chinensis, 10-25 parts of Codonopsis pilosula, 10-25 parts of Poria cocos, 10-25 parts of stir-fried Atractylodes macrocephala, 10-25 parts of charred Terminalia chebula, 6-17 parts of Alisma orientalis, 6-17 parts of dried ginger and 6-17 parts of Licorice; in the Chinese medicine compound mixture, the mass percentage of the Chinese medicine composition is 80%; The probiotics are composed of lactobacillus, yeast, bacillus subtilis and bacillus licheniformis. After fermenting lactobacillus, yeast, bacillus subtilis and bacillus licheniformis respectively, the lactobacillus fermentation liquid, the yeast fermentation liquid, the bacillus subtilis fermentation liquid and the bacillus licheniformis fermentation liquid are mixed at a volume ratio of 1:1:1:1 to obtain a probiotic fermentation liquid, which is then added to a fermentation substrate for fermentation, and the fermentation product is freeze-dried and crushed to obtain a probiotic fermentation Chinese medicine product for treating porcine Escherichia coli diarrhea; The lactobacillus is Lactobacillus fermentum, with a preservation number of CGMCC No.4648; the yeast is Saccharomyces cerevisiae, with a preservation number of CGMCC No.16952; the Bacillus subtilis has a preservation number of CGMCC No.3440; and the Bacillus licheniformis has a preservation number of CGMCC No.7388; The viable counts of the fermented lactobacillus, saccharomyces cerevisiae, bacillus subtilis and bacillus licheniformis are not less than 1×10 9 CFU / mL.

2. The probiotic fermented Chinese medicine product for treating swine Escherichia coli diarrhea according to claim 1, characterized in that: The Chinese medicine composition is prepared from the following Chinese medicine raw materials in parts by weight: 15 parts of Pueraria root, 9 parts of Scutellaria baicalensis, 9 parts of Coptis chinensis, 15 parts of Codonopsis pilosula, 15 parts of Poria cocos, 15 parts of stir-fried Atractylodes macrocephala, 15 parts of charred Terminalia chebula, 10 parts of Alisma orientalis, 10 parts of dried ginger and 10 parts of liquorice.

3. A method for preparing a probiotic fermented Chinese medicine product for treating swine Escherichia coli diarrhea as claimed in any one of claims 1 to 2, characterized in that: The following steps are involved: (1) According to the dosage of the formula, kudzu root, scutellaria baicalensis, coptis root, codonopsis pilosula, poria, stir-fried atractylodes macrocephala, charred terminalia chebula, oriental water chestnut, dried ginger and liquorice are crushed and sieved, and then soybean meal is added and mixed evenly to obtain a traditional Chinese medicine compound mixture; Mixing and culturing a probiotic mixed bacterial liquid containing lactobacillus, yeast, Bacillus subtilis and Bacillus licheniformis with the traditional Chinese medicine compound mixture to obtain a fermentation product of the compound traditional Chinese medicine and the probiotic mixture; (2) freeze-drying and crushing the fermented product of the compound Chinese medicine and the probiotic mixture to obtain a probiotic fermented Chinese medicine product for treating porcine Escherichia coli diarrhea; The probiotic mixed bacterial liquid is prepared by fermenting lactobacillus, yeast, bacillus subtilis and bacillus licheniformis respectively, and then mixing the lactobacillus fermentation liquid, yeast fermentation liquid, bacillus subtilis fermentation liquid and bacillus licheniformis fermentation liquid in a volume ratio of 1:1:1:

1.

4. The preparation method according to claim 3, characterized in that: (1) was sieved with a 60-mesh sieve.

5. The preparation method according to claim 3, characterized in that: (1), 5-80 mL of the probiotic mixed bacterial solution is added to every 1 kg of the traditional Chinese medicine compound mixture, and 320-395 mL of distilled water is added at the same time, and the mixture is mixed evenly and then mixed and cultured.

6. The preparation method according to claim 3, characterized in that: The mixed culture conditions are: fermentation at 35° C. for 48 hours.

7. Use of the probiotic fermented Chinese medicinal product according to any one of claims 1 to 2 in the preparation of a medicine or feed additive for treating diarrhea caused by porcine Escherichia coli.

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