Multi-mouse fixation device and multi-mouse magnetic resonance imaging method

Abstract

The application discloses a multi-mouse fixing device and a multi-mouse magnetic resonance imaging method, and belongs to the technical field of magnetic resonance imaging. The multi-mouse fixing device comprises a fixing device, at least two cavities are arranged in the fixing device, and the fixing device can be fixed in a magnetic resonance birdcage coil. The multi-mouse supporting module comprises at least two mouse supporting beds, the mouse supporting beds are slidingly arranged in the cavities, the mouse supporting beds are concavely provided with accommodating grooves, anesthetic masks are slidingly arranged on groove edge portions of opposite sides of the accommodating grooves, the anesthetic masks are provided with anesthetic openings, first ends of telescopic tooth bars are connected with groove bottom portions of the accommodating grooves, second ends of the telescopic tooth bars pass through the anesthetic openings, and the second ends are used for fixing the heads of experimental mice. The application can quickly adjust the positions of the experimental mice according to actual use conditions, so that imaging results of different organs are obtained, at least two experimental mice can be imaged at one time, the experimental efficiency is improved, and large sample quantity repetitive experiments can be facilitated.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of nuclear magnetic resonance imaging, in particular to a multi-mouse fixing device and a multi-mouse nuclear magnetic resonance imaging method. BACKGROUND

[0002] Small animal nuclear magnetic resonance is very suitable for morphological and pathophysiological research of experimental mice due to its high spatial resolution, good imaging effect on soft tissue, and the ability to perform whole-body scanning on experimental mice in a non-destructive manner.

[0003] During the imaging process of a large number of experimental mice tissue morphological research using a small animal nuclear magnetic resonance instrument, two problems were found that need to be solved. First, when conducting in vivo imaging research of drug metabolism or new nanomaterials, it is necessary to track the target organs and metabolic pathways of the drugs, which often requires imaging of multiple organs such as the liver, lungs, spleen, kidneys, and bladder. This requires that the experimental mice be within the effective imaging range of the birdcage coil of the small animal nuclear magnetic resonance. The conventional mouse bed requires manual replacement of different lengths of adjusting tooth bars to change the position of the experimental mice in the birdcage coil of the nuclear magnetic resonance, thereby obtaining imaging results of different organs. Second, in the development of new drugs, multiple experimental groups and control groups are required, with at least 3 experimental mice in each group. The conventional experimental mouse fixing device can only satisfy the imaging of one experimental mouse at a time, and the experimental efficiency is relatively low when facing large sample size repetitive experiments.

[0004] Therefore, it is urgent to provide a multi-mouse fixing device and a multi-mouse nuclear magnetic resonance imaging method to solve the above problems. SUMMARY

[0005] The purpose of the present application is to provide a multi-mouse fixing device and a multi-mouse nuclear magnetic resonance imaging method that can quickly adjust the position of experimental mice according to actual use, thereby obtaining imaging results of different organs, satisfying the imaging of at least two experimental mice at a time, improving experimental efficiency, and facilitating large sample size repetitive experiments.

[0006] To achieve the above purpose, the following technical solutions are provided:

[0007] The multi-mouse fixing device comprises:

[0008] A fixing device is provided with at least two cavities inside; the fixing device can be fixed in the birdcage coil of the nuclear magnetic resonance;

[0009] A multi-mouse support module comprises at least two mouse support beds, which are slidingly arranged in the cavities. The mouse support bed is concavely provided with a receiving groove, and anesthetic masks are slidingly arranged on the groove edges of the opposite sides of the receiving groove. The anesthetic masks are provided with anesthetic openings. The first end of an extendable tooth bar is connected to the groove bottom of the receiving groove, and the second end of the extendable tooth bar passes through the anesthetic opening to fix the head of the experimental mouse.

[0010] As an alternative to the multi-mouse fixing device, the outer wall surface of the opposite sides of the anesthesia mask is provided with a guide protrusion, which can slide along the groove edge, and the groove edge is provided with a scale line.

[0011] As an alternative to the multi-mouse fixing device, a hose is further included, the anesthesia mask is provided with a gas passage, the exhaust port of the gas passage is communicated with the anesthesia port, one end of the hose is communicated with the air inlet of the gas passage, and the other end of the hose is communicated with the anesthesia machine.

[0012] As an alternative to the multi-mouse fixing device, the anesthesia port includes a first opening and a second opening in communication, the inner diameter of the second opening is larger than that of the first opening, and the exhaust port of the gas passage is communicated with the second opening.

[0013] As an alternative to the multi-mouse fixing device, the first buckle plate part is provided on one end of the fixing device in a circumferential direction, the first buckle plate part is stopped at the socket of the nuclear magnetic birdcage coil, and a horizontal marker line is provided on the first buckle plate part.

[0014] As an alternative to the multi-mouse fixing device, the inner wall surface of the cavity is recessed with a guide chute, and the outer edge of the groove edge is slidably arranged in the guide chute.

[0015] As an alternative to the multi-mouse fixing device, the mouse support bed is provided with a second buckle plate part on one end away from the groove bottom in a circumferential direction, the cavity is recessed with a blocking part on one end close to the first buckle plate part, and the second buckle plate part is stopped at the blocking part.

[0016] As an alternative to the multi-mouse fixing device, the outer wall surface of the groove bottom is recessed with a limiting groove, the groove bottom of the limiting groove is provided with a through hole, the tooth rod fixing body of the telescopic tooth rod is arranged through the through hole, and the first end of the telescopic tooth rod is embedded in the limiting groove.

[0017] As an alternative to the multi-mouse fixing device, a fastener is further included, the first end of the telescopic tooth rod is provided with a first connecting hole, the groove bottom of the limiting groove is provided with a second connecting hole, and the fastener is connected through the first connecting hole and the second connecting hole.

[0018] A multi-mouse nuclear magnetic resonance imaging method, using a small animal nuclear magnetic resonance imaging instrument and a multi-mouse fixing device as described above to perform multi-mouse imaging, includes the following steps:

[0019] S1, according to the required number of animal experiments, the experimental mice are fixed on the mouse support bed through the telescopic tooth rod;

[0020] S2, pull down the anesthesia mask connected with the hose and wrap the head of the experimental mouse, adjust the mixed gas of anesthesia gas and oxygen to the set ratio through the anesthesia machine, maintain the anesthesia state of the experimental mouse, and fix the anesthesia mask on the mouse support bed through the adhesive tape;

[0021] S3, respectively insert the three mouse support beds into the corresponding cavities and push into the preset position of the fixer;

[0022] S4, push the whole multi-mouse fixing device into the nuclear magnetic birdcage coil of the small animal nuclear magnetic resonance imaging instrument, and buckle the fixer with the second connecting hole nuclear magnetic birdcage coil;

[0023] S5, push the nuclear magnetic birdcage coil containing the multi-mouse fixing device into the imaging center of the magnet of the small animal nuclear magnetic resonance imaging instrument, and perform nuclear magnetic scanning.

[0024] Compared with the prior art, the beneficial effects of the present application are:

[0025] The multi-mouse fixing device provided by the present application installs a multi-mouse support module into a fixer for simultaneous detection of multiple experimental mice. At least two cavities are arranged in the fixer, and a mouse support bed is placed in each cavity to satisfy imaging of at least two experimental mice at a time, improve experimental efficiency, and facilitate large sample size repetitive experiments. An experimental mouse is placed in a containing groove of the mouse support bed, an anesthesia mask is slidingly arranged at the groove edge of the containing groove, a first end of a telescopic tooth bar is installed at the groove bottom of the containing groove, and a second end of the telescopic tooth bar is used to fix the head of the experimental mouse. After the experimental mouse is placed in the containing groove, the head and teeth of the experimental mouse are fixed to the second end, and then the anesthesia mask is pulled to anesthetize the head of the experimental mouse with gas, so that the experimental mouse enters an anesthesia state. The length of the telescopic tooth bar is adjustable, and the position of the experimental mouse can be quickly adjusted according to the body length of the experimental mouse, without the need to replace tooth bars of different lengths, and imaging results of different organs can also be obtained.

[0026] The multi-mouse nuclear magnetic resonance imaging method provided by the present application not only satisfies imaging of at least two experimental mice at a time, improves experimental efficiency, and facilitates large sample size repetitive experiments, but also facilitates quick change of the position of the experimental mouse to realize clear imaging of different organs. BRIEF DESCRIPTION OF DRAWINGS

[0027] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings needed in the description of the embodiments of the present application will be briefly introduced. Obviously, the drawings in the following description are only some embodiments of the present application, and other drawings can be obtained by those skilled in the art without creative labor on the basis of the contents of the embodiments of the present application and these drawings.

[0028] Figure 1 Structure diagram of the fixed device for the multi-mouse supporting module in the embodiment of the present application;

[0029] Figure 2 Structure diagram of the fixed device for the multi-mouse supporting module in the embodiment of the present application;

[0030] Figure 3 Structure diagram of the fixed device for the multi-mouse supporting module in the embodiment of the present application;

[0031] Figure 4 Structure diagram of the fixed device for the multi-mouse supporting module in the embodiment of the present application;

[0032] Figure 5 Structure diagram of the fixed device for the multi-mouse supporting module in the embodiment of the present application;

[0033] Figure 6 Structure diagram of the fixed device for the multi-mouse supporting module in the embodiment of the present application;

[0034] Figure 7 Structure diagram of the fixed device for the multi-mouse supporting module in the embodiment of the present application;

[0035] Figure 8 Structure diagram of the fixed device for the multi-mouse supporting module in the embodiment of the present application;

[0036] Figure 9 Structure diagram of the fixed device for the multi-mouse supporting module in the embodiment of the present application;

[0037] Reference signs:

[0038] 1, fixed device; 2, multi-mouse supporting module;

[0039] 11, cavity; 12, first buckle plate part; 13, horizontal mark line; 14, guide sliding groove; 15, blocking part; 16, avoiding slot;

[0040] 21, mouse supporting bed; 22, anesthesia mask; 23, telescopic tooth bar; 24, hose; 25, fastener;

[0041] 211, accommodating groove; 212, groove edge part; 213, groove bottom part; 214, scale line; 215, second buckle plate part; 216, limiting slot; 217, through hole; 218, second connecting hole;

[0042] 221, anesthesia port; 2211, first opening; 2212, second opening; 222, gas passage; 223, exhaust port; 224, air inlet; 225, guide protrusion;

[0043] 231, first end; 232, tooth bar fixed body; 233, second end; 234, first connecting hole; 235, fixing hole; 236, tooth bar telescopic body. DETAILED DESCRIPTION

[0044] In order to make the objects, technical solutions and advantages of the embodiments of the present application clearer, the technical solutions in the embodiments of the present application will be described clearly and completely below in conjunction with the accompanying drawings in the embodiments of the present application. Obviously, the described embodiments are only some of the embodiments of the present application, rather than all the embodiments. The components of the embodiments of the present application described and shown in the accompanying drawings can be arranged and designed in various different configurations.

[0045] In the description of the present application, it should be noted that the terms "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, or the orientation or positional relationship when the product of the present application is usually placed, and are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the indicated device or element must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as a limitation on the present application. In addition, the terms "first", "second", "third" and the like are only used for differentiation in description and cannot be understood as indicating or implying relative importance. In the description of the present application, unless otherwise specified, the meaning of "a plurality of" is two or more.

[0046] In the description of the present application, it should also be noted that, unless otherwise specified and limited, the terms "arrangement", "connection" should be understood broadly, for example, it can be fixedly connected, or it can be detachably connected, or integrally connected; it can be mechanically connected, or it can be electrically connected. For those skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

[0047] The embodiments of the present application will be described in detail below, and examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals represent the same or similar elements or elements having the same or similar functions throughout. The embodiments described below by reference to the accompanying drawings are exemplary and are only used to explain the present application, and cannot be understood as a limitation on the present application.

[0048] In order to be able to quickly adjust the position of the experimental mouse according to the actual use, so as to obtain the imaging results of different organs; meet at least two experimental mouse imaging at a time, improve the experimental efficiency, help to carry out large sample quantity repetitive experiment, the present embodiment provides a multi-mouse fixing device and a multi-mouse magnetic resonance imaging method, which will be described below in conjunction with Figures 1 to 9 The specific content of the present embodiment will be described in detail.

[0049] As Figures 1 to 8As shown, the multi-mouse fixing device in the embodiment includes a fixer 1 and a multi-mouse supporting module 2. The fixer 1 is in a cylindrical structure and is made of polytetrafluoroethylene material. Different sizes of the fixer 1 can be designed according to the inner diameter and length of the nuclear magnetic birdcage coil. In order to fully support and fill, the length and outer diameter of the fixer 1 located in the nuclear magnetic birdcage coil can be adapted and manufactured according to the size of the nuclear magnetic birdcage coil of the corresponding small animal magnetic resonance imaging instrument. At least two cavities 11 are arranged in the fixer 1. The fixer 1 can be fixed in the nuclear magnetic birdcage coil. The multi-mouse supporting module 2 is composed of a mouse supporting bed 21, a telescopic jaw 23 and an anesthesia mask 22. The multi-mouse supporting module 2 includes at least two mouse supporting beds 21. The mouse supporting bed 21 is made of polytetrafluoroethylene material. The mouse supporting bed 21 can be pushed into the cavity 11 of the fixer 1, and the nuclear magnetic imaging detection of multiple experimental mice can be performed at the same time. The mouse supporting bed 21 is slidingly arranged in the cavity 11. The mouse supporting bed 21 is concavely provided with an elongated accommodating groove 211. The inner diameter and depth of the accommodating groove 211 are designed according to the circumference of the experimental mouse to be accommodated. The bed length of the mouse supporting bed 21 is designed according to the effective imaging range of the nuclear magnetic birdcage coil and the body length of the experimental mouse. The anesthesia mask 22 is slidingly arranged on the groove edge part 212 of the relative two sides of the accommodating groove 211.

[0050] Specifically, the anesthesia mask 22 is in a cylindrical structure. Two guide protrusions 225 are protrudingly arranged on the outer walls of the relative two sides of the anesthesia mask 22. The guide protrusions 225 can slide along the groove edge part 212. The scale line 214 is arranged on the groove edge part 212. The bed body has the scale line 214, which is convenient for determining the body length of the experimental mouse and the imaging part. The anesthesia mask 22 is provided with an anesthesia port 221. The telescopic jaw 23 includes a first end 231, a jaw fixed body 232, a jaw telescopic body 236 and a second end 233. The jaw telescopic body 236 is movably sleeved in the pipe opening of the jaw fixed body 232. The first end 231 is arranged on the side of the jaw fixed body 232 away from the jaw telescopic body 236 (in the embodiment, the first end 231 is in an integrated structure with the jaw fixed body 232). The second end 233 is arranged on the side of the jaw telescopic body 236 away from the jaw fixed body 232 (in the embodiment, the second end 233 is in an integrated structure with the jaw telescopic body 236. The second end 233 is provided with a fixing hole 235). The first end 231 of the telescopic jaw 23 is connected with the groove bottom part 213 of the accommodating groove 211. The second end 233 of the telescopic jaw 23 penetrates through the anesthesia port 221 and is used for fixing the head of the experimental mouse.

[0051] In the embodiment, the inside of the fixer 1 includes three cylindrical cavities 11 capable of accommodating the mouse supporting bed 21. The length of the cavity 11 is consistent with the length of the fixer 1.

[0052] Further, the inner wall surface of the cavity 11 on both sides is concavely provided with a guide chute 14, and the outer edge of the groove edge part 212 is slidingly arranged in the guide chute 14. By additionally arranging the guide chute 14 in the cavity 11, the groove edge part 212 at both ends of the mouse supporting bed 21 can be inserted, and the mouse supporting bed 21 can slide in the cavity 11 of the fixer 1. The fixer 1 is circumferentially provided with a first buckle plate part 12 at one end, and the first buckle plate part 12 is stopped at the insertion port of the nuclear magnetic birdcage coil. The first buckle plate part 12 is inlaid with the insertion port at the bottom of the nuclear magnetic birdcage coil, and is used to fix the multi-mouse fixing device in the nuclear magnetic birdcage coil. The horizontal marker line 13 is arranged on the first buckle plate part 12, which is used to adjust the fixer 1 and the nuclear magnetic birdcage coil to the same horizontal position.

[0053] In short, the multi-mouse fixing device provided by the embodiment is used for simultaneous detection of multiple experimental mice. The multi-mouse supporting module 2 is installed in the fixer 1, and at least two cavities 11 are arranged in the fixer 1. The mouse supporting bed 21 is arranged in each cavity 11, which can satisfy the imaging of at least two experimental mice at a time, improve the experimental efficiency, and help to conduct large sample quantity and repetitive experiments. The experimental mouse is placed in the accommodation groove 211 of the mouse supporting bed 21, the anesthesia mask 22 is slidingly arranged on the groove edge part 212 of the accommodation groove 211, the first end 231 of the telescopic tooth bar 23 is installed on the groove bottom 213 of the accommodation groove 211, and the second end 233 of the telescopic tooth bar 23 is used to fix the head of the experimental mouse. After the experimental mouse is placed in the accommodation groove 211, the head and teeth of the experimental mouse are fixed to the second end 233, and then the anesthesia mask 22 is pulled to gas anesthetize the head of the experimental mouse, so that the experimental mouse enters an anesthetized state. The length of the telescopic tooth bar 23 is adjustable, and the position of the experimental mouse can be quickly adjusted according to the size of the experimental mouse, without the need to replace tooth bars of different lengths, and imaging results of different organs can also be obtained.

[0054] Further, as Figure 5 , Figure 6 and Figure 9As shown, the multi-mouse fixing device further comprises a hose 24, the anesthesia mask 22 is provided with a gas passage 222, an exhaust port 223 of the gas passage 222 is communicated with an anesthesia port 221, one end of the hose 24 is communicated with an air inlet 224 of the gas passage 222, and the other end of the hose 24 is communicated with an anesthesia machine. Further, the anesthesia port 221 comprises a first opening 2211 and a second opening 2212 communicated with each other, an inner diameter of the second opening 2212 is larger than that of the first opening 2211, and the exhaust port 223 of the gas passage 222 is communicated with the second opening 2212. The first opening 2211 is used for penetrating the tooth bar fixing body 232 of the telescopic tooth bar 23. The diameter of the second opening 2212 is designed according to the head circumference of the experimental mouse, and is used for accommodating the head of the experimental mouse. The exhaust port 223 of the gas passage 222 is communicated with the second opening 2212 for accommodating the head of the experimental mouse, and is used for supplying anesthetic gas. The air inlet 224 of the gas passage 222 is connected with a commercial anesthesia machine through the hose 24, the anesthesia machine is used for adjusting the mixed gas of anesthetic gas and oxygen to a suitable proportion, and maintaining the anesthesia state of the experimental mouse. The guide protrusions 225 on both sides of the anesthesia mask 22 can be placed on the mouse support bed 21 to avoid rolling over, and then fixed in a suitable position by a tape. In other embodiments, the anesthesia mask 22 can also be fixed in a suitable position of the mouse support bed 21 by using a screw.

[0055] Further, the mouse support bed 21 is provided with a second buckle plate portion 215 at an end away from the groove bottom portion 213, the cavity 11 is provided with a blocking portion 15 at an end close to the first buckle plate portion 12, and the second buckle plate portion 215 is stopped at the blocking portion 15. Through the cooperation of the two buckle plate portions and the blocking portion 15, the position of the mouse support bed 21 is limited, so that the mouse support bed 21 is prevented from being inserted into the cavity 11 too much.

[0056] Further, the outer wall surface of the groove bottom portion 213 is concavely provided with a limiting groove 216, the limiting groove 216 is provided with a through hole 217, the tooth bar fixing body 232 of the telescopic tooth bar 23 penetrates through the through hole 217, and the first end 231 of the telescopic tooth bar 23 is embedded in the limiting groove 216. Specifically, the limiting groove 216 and the first end 231 are both in a gourd shape, and the position of the first end 231 in the limiting groove 216 is limited.

[0057] Further, the multi-mouse fixing device further comprises a fastener 25, the first end 231 of the telescopic tooth bar 23 is provided with a first connecting hole 234, the groove bottom of the limiting groove 216 is provided with a second connecting hole 218, and the fastener 25 penetrates through the first connecting hole 234 and the second connecting hole 218 to be connected. When the fastener 25 is a screw, the fastener 25 is threadedly connected through the first connecting hole 234 and the second connecting hole 218; when the fastener 25 is a rivet, the fastener 25 is riveted through the first connecting hole 234 and the second connecting hole 218.

[0058] The embodiment also provides a multi-mouse nuclear magnetic resonance imaging method using a small animal nuclear magnetic resonance imaging instrument and the multi-mouse fixing device mentioned above, and the multi-mouse nuclear magnetic resonance imaging method comprises the following steps:

[0059] S1, according to the required number of animal experiments, fixing the experimental mice on the mouse support bed 21 through the telescopic tooth bar 23;

[0060] S2, pulling down the anesthesia mask 22 connected with the hose 24, fully wrapping the head of the experimental mouse, adjusting the mixed gas of anesthetic gas and oxygen to a set ratio through the anesthesia machine, maintaining the anesthesia state of the experimental mouse, and fixing the anesthesia mask 22 on the mouse support bed 21 through the adhesive tape;

[0061] S3, respectively inserting the three mouse support beds 21 into the corresponding cavities 11 and pushing into the preset position of the fixer 1;

[0062] S4, pushing the whole multi-mouse fixing device into the nuclear magnetic birdcage coil, and tightly buckling the fixer 1 and the second connecting hole 218 of the nuclear magnetic birdcage coil;

[0063] S5, pushing the nuclear magnetic birdcage coil containing the multi-mouse fixing device into the imaging center of the magnet, and performing nuclear magnetic scanning.

[0064] Compared with the prior art, the multi-mouse fixing device has the following beneficial effects:

[0065] (1) Compared with the existing detection method, the multi-mouse fixing device can simultaneously fix, anesthetize and image scan multiple experimental mice. Under the same scanning sequence and scanning times, more sample quantities can be scanned at one time, the imaging detection efficiency is improved, and the complexity of the later data processing and image fusion is greatly reduced.

[0066] (2) Compared with the existing experimental mouse bed, each mouse support bed 21 of the multi-mouse fixing device has a scale prompt, which helps to determine the placement position of the experimental mouse, such as the positioning of the imaging main body (subcutaneous tumor, head and abdominal surgical site, lower limb joint, etc.) and the imaging center of the magnetic field, and improves the imaging accuracy.

[0067] (3) Compared with the existing experimental mouse bed, the multi-mouse fixing device designs an additional fixer 1, which can tightly fix each mouse support bed 21 in the nuclear magnetic birdcage coil, avoiding motion artifacts caused by scanning vibration. The guide sliding groove 14 in the fixer 1 and the horizontal marker line 13 at the tail can ensure that the experimental mouse is horizontally prone or supine in the nuclear magnetic birdcage coil, and will not produce a large turning angle, thereby improving the imaging quality.

[0068] (4) Compared with the existing detection method, the telescopic tooth bar 23 is adopted in the present application, and only the upper teeth of the experimental mouse need to be buckled into the fixing hole 235 of the second end 233 during imaging, the anesthesia mask 22 is pulled down, and the position of the anesthesia mask 22 is fixed by using a screw or adhesive tape. The imaging of the whole body of the experimental mouse at any position can be completed without the need of adjusting or replacing the position of the tooth bar on the mouse support bed 21. This greatly simplifies the manual positioning process and the number of repeated positioning scans, and improves the positioning accuracy and efficiency.

[0069] It should be noted that the above are only the preferred embodiments of the present application and the principles of the technology applied. Those skilled in the art will understand that the present application is not limited to the specific embodiments described herein, and those skilled in the art can make various obvious changes, readjustments and substitutions without departing from the scope of the present application. Therefore, although the present application has been described in more detail through the above embodiments, the present application is not limited to the above embodiments, and can include more other equivalent embodiments without departing from the concept of the present application, and the scope of the present application is determined by the scope of the appended claims.

Claims

1. A multi-mouse restraint device, characterized in that, include: Fixture (1), wherein the fixture (1) is provided with at least two cavities (11); the fixture (1) can be fixed inside the nuclear magnetic birdcage coil; one end of the fixture (1) is provided with a first buckle plate (12) protruding circumferentially, the first buckle plate (12) stops at the insertion port of the nuclear magnetic birdcage coil, and a horizontal marking line (13) is provided on the first buckle plate (12); A multi-rodent support module (2) includes at least two rodent support beds (21), which are slidably disposed within the cavity (11). Each rodent support bed (21) has a recessed receiving groove (211). Anesthesia masks (22) are slidably disposed on the groove edges (212) on opposite sides of the receiving groove (211). The anesthesia masks (22) have anesthesia ports (221). The first end (231) of a telescopic dental rod (23) is connected to the bottom (213) of the receiving groove (211). The telescopic dental rod (23) includes a first end (231), a dental rod fixing body (232), a dental rod telescopic body (236), and a second end (233). The telescopic rod (236) is movably fitted inside the opening of the rod fixation body (232); the second end (233) of the telescopic rod (23) passes through the anesthesia port (221) and is used to fix the head of the experimental mouse; the inner wall of the cavity (11) is recessed with a guide groove (14), and the outer edge of the groove edge (212) is slidably disposed in the guide groove (14); the end of the mouse support bed (21) away from the bottom (213) of the groove is circumferentially provided with a second buckle plate (215), and the end of the cavity (11) near the first buckle plate (12) is recessed with a blocking part (15), and the second buckle plate (215) stops at the blocking part (15); The anesthesia mask (22) has a gas channel (222) inside, the exhaust port (223) of the gas channel (222) is connected to the anesthesia port (221), one end of the hose (24) is connected to the air inlet (224) of the gas channel (222), the air inlet (224) is located on the end face of the anesthesia mask (22) away from the bottom of the groove (213), and the other end of the hose (24) is connected to the anesthesia machine; The anesthesia mask (22) has guide protrusions (225) on the outer wall surfaces on both sides. The guide protrusions (225) can slide along the groove edge (212). The groove edge (212) is provided with scale lines (214). The multi-mouse immobilization device is applied to a multi-mouse magnetic resonance imaging method, including the following steps: S1. Based on the number required for the animal experiment, fix the experimental mice to the mouse support bed (21) using telescopic tooth rods (23); S2. Pull down the anesthesia mask (22) of the connecting tube (24) and wrap it around the head of the experimental mouse. Adjust the mixture of anesthetic gas and oxygen to the set ratio through the anesthesia machine to maintain the anesthesia state of the experimental mouse. Fix the anesthesia mask (22) on the mouse support bed (21) with tape. S3. Insert the three rodent support beds (21) into the corresponding cavities (11) and push them into the preset positions in the fixture (1); S4. Push the entire multi-mouse fixing device into the nuclear magnetic birdcage coil of the small animal nuclear magnetic resonance imaging instrument, and fasten the fixing device (1) to the nuclear magnetic birdcage coil of the second connecting hole (218). S5. The magnetic resonance imaging coil containing the multi-mouse restraint device is pushed into the magnetic imaging center of the small animal magnetic resonance imaging instrument for magnetic resonance scanning.

2. The multi-rat restraint device according to claim 1, characterized in that, The anesthesia port (221) includes a first opening (2211) and a second opening (2212) that are connected. The inner diameter of the second opening (2212) is larger than the inner diameter of the first opening (2211). The exhaust port (223) of the gas channel (222) is connected to the second opening (2212).

3. The multi-rat restraint device according to claim 1, characterized in that, The outer wall surface of the bottom (213) of the groove is recessed with a limiting groove (216), the bottom of the limiting groove (216) is provided with a through hole (217), the tooth rod fixing body (232) of the telescopic tooth rod (23) passes through the through hole (217), and the first end (231) of the telescopic tooth rod (23) is embedded in the limiting groove (216).

4. The multi-mouse restraint device according to claim 3, characterized in that, It also includes a fastener (25), the first end (231) of the telescopic toothed rod (23) is provided with a first connecting hole (234), the bottom of the limiting groove (216) is provided with a second connecting hole (218), and the fastener (25) passes through the first connecting hole (234) and connects with the second connecting hole (218).

Images