Additive for preserving cells, tissues and / or food
By using synthetic peptide additives containing specific amino acid sequences and cell-penetrating peptide tails, the problem of preserving cells, tissues, and food has been solved, achieving the effects of preventing harmful organisms from attacking and extending shelf life.
Patent Information
- Application Number
- CN202280013788.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Priority Date
- 2021-02-08
- Filing Date
- 2022-02-04
- Publication Date
- 2026-05-15
- Estimated Expiration
- 2042-02-04
AI Technical Summary
There is a lack of effective methods in the current technology for preserving cells, tissues and food, preventing the invasion of harmful organisms, extending shelf life, and improving food safety and plant quality.
Synthetic peptides are used as additives, containing peptides with specific amino acid sequences and cell-penetrating peptide tails. They are applied externally to cells, tissues, and food to prevent harmful organisms from attacking, extend shelf life, and regulate germination time.
It significantly extends the shelf life of fruits, plants, and food, prevents rot and wilting, and improves food safety and plant preservation.
Smart Images
Figure CN116887674B_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the fields of biotechnology, phytochemistry, and food processing, and provides an additive for preserving cells, tissues, and / or food, as well as the use of synthetic peptides in the preparation of said additive. Among other applications, this invention can be used to increase the shelf life of food and provide enhanced food safety. In one embodiment, the additive of this invention provides substantial preservation of fruit through a single application. This invention provides treatments for plants or food to prevent the presence of harmful organisms such as bacteria or fungi, including those associated with the decay of fruits (including strawberries) and Xylella fastidiosa in coffee and orange trees. In one embodiment, the additive of the present invention provides substantial preservation of flowers or inflorescences by a single application, providing an alternative to rapid wilting, decay, or fungal growth associated with the storage, transport, and / or pharmaceutical processing of flowers or inflorescences of phytochemical value (including gerbera, cannabis, etc.). In one embodiment, the additive of the present invention provides protection for buds or seedlings when applied. Substantial preservation of metabolic state and slowing down seed germination The process can therefore be used for synchronized germination, adjusting the timing of planting or germination, and has advantages involving other agronomic or botanical aspects. Background Technology
[0002] The phytochemical and food markets need to continuously evolve to meet the demands of production, logistics, and consumption. In this context, solutions that benefit any of these points in the chain are highly desirable. This invention provides a solution applicable to all these elements across various parts of the chain.
[0003] While searching prior art scientific and patent literature, the following documents related to this topic were found:
[0004] The inventor's patent application BR 102014000521-0 is considered the closest prior art to this invention. However, neither this document nor any other document found discloses, suggests, or teaches the external use of peptides for cell preservation and / or food (uso tópico), nor additives containing said synthetic peptides.
[0005] As can be seen from the literature reviewed, no literature was found that anticipated or suggested the teachings of this invention. Therefore, in the view of the inventors, the solution proposed in this application is novel and inventive relative to the prior art. Summary of the Invention
[0006] This invention addresses the problems of the prior art by using synthetic peptides for preserving cells, tissues and / or food, and additives containing said peptides, and can be used to improve the production, logistics and / or consumption of food and / or plants.
[0007] One of the objectives of this invention is to provide a method to increase the shelf life of food and provide greater food safety.
[0008] Another object of the present invention is to provide a method to improve the quality and / or shelf life of plants or parts thereof, thereby providing improvements to the production chain of phytochemical products.
[0009] In one embodiment, the present invention provides a topical additive comprising the peptide and water or excipient for preserving cells, tissues and / or food.
[0010] In one embodiment, the present invention provides an additive for use in the hydration solution of stems or branches of flowers or inflorescences that have ornamental or phytochemical value.
[0011] The synthetic peptides of this invention are selected from the peptides of SEQ ID NO:1 and SEQ ID NO:4 to 14, and optionally additionally include a cell-penetrating peptide tail at the N-terminal and / or C-terminal portions of the first and / or last amino acids. The peptide of SEQ ID NO:3 represents a peptide comprising the peptide of SEQ ID NO:1 and the cell-penetrating peptide of SEQ ID NO:2 in the C-terminal region. The peptide of SEQ ID NO:15 represents a peptide comprising the peptide of SEQ ID NO:9 and the cell-penetrating peptide of SEQ ID NO:2.
[0012] In one embodiment, the additive of this application provides substantial preservation of fruit when applied in at least one dose.
[0013] In one embodiment, the additive of this application provides a treatment of plants or food to prevent the presence of harmful organisms (pragas) such as bacteria or fungi, for example those associated with the rot or contamination of strawberries.
[0014] In one embodiment, when the additive is applied, the additive of this application provides substantial preservation of the metabolic state of buds or seedlings, as well as the seed germination process, and can therefore be used for synchronized germination, adjustment of planting or germination timing, and other agronomic or botanical advantages.
[0015] In one embodiment, the additive of this application provides a treatment of flowers or inflorescences of ornamental or phytochemical value to prevent or delay wilting, rotting, or the appearance of harmful organisms (such as bacteria or fungi), and to maintain the quality and integrity of the flowers or inflorescences for a longer period of time.
[0016] The foregoing and other objectives of this invention will soon be recognized by those skilled in the art and by companies interested in this part, and will be described in full detail in order to reproduce them below. Attached Figure Description
[0017] To better define and illustrate the contents of this patent application, the following images are provided:
[0018] Figure 1 Visual results are shown for experiments involving the application of 100 μM preservative peptide Ktut, SEQ ID NO:3, or water (control) to fresh strawberries in the Ktut implementation scheme. All strawberries were from the same batch and the same commercial product packaging. Results for each group of 10 strawberries under each condition (experiment and control) at 0, 12, 24, 36, 48, and 60 h are shown in A, B, C, D, E, and F, respectively.
[0019] Figure 2 The figure shows the time (horizontal axis) and percentage of spoiled fruit (vertical axis (ordenadas)) after applying 100 μM preservative peptide (Ktut, SEQ ID NO:3) or water to fresh strawberries.
[0020] Figure 3 Visual results of experiments involving the application of the additive of the present invention are shown, wherein an aqueous solution of one embodiment of the preservative peptide (Ktut, SEQ ID NO:3) was applied to eight fresh strawberries in each group, or water was applied as a control. All strawberries were from the same batch and the same commercial product packaging. Representative results of strawberries under each condition (experimental and control) at 0h, 24h, 48h, and 5 days are shown in A, B, C, and D, respectively.
[0021] Figure 4Visual results are shown of experiments involving the application of a single dose of 100 μM (aqueous solution) of the preservative peptide (Ktut, SEQ ID NO:3) or water (control) to legume seeds. All seeds were from the same batch and packaged in the same commercial product and stored at room temperature (28°C). Germination results of each group of 14 seeds in moist cotton at 0, 3, and 4 days under each condition (control and experimental) are shown in A, B, and C, respectively. The images illustrate the differences in germination patterns among the legumes.
[0022] Figure 5 It shows in Figure 4 Under the conditions shown, time in days (horizontal axis) and number of germinated legume seeds (vertical axis) after application of 100 μM preservative peptide in one implementation scheme (Ktut, SEQ ID NO:3).
[0023] Figure 6 Visual results are shown of an experiment in which a single dose of a 100 μM (aqueous solution) preservative peptide (Ktut, SEQ ID NO: 15) or water (control) was applied to two types of flowers. All flowers were from the same batch and acquired on the same day, and stored at room temperature (28°C). Four flowers were used in each container, with the stems kept submerged in the liquid at least 5 cm deep during the experiment. Aluminum foil was used around the containers where the stems were submerged to prevent light exposure. Photographic results of the flowers stored under each condition (control and experimental) at 0, 3, and 6 days are shown. Images of roses at 0, 3, and 6 days are shown in A, C, and E, with the rose on the left stored in water and the rose on the right stored in the aforementioned preservative peptide. Images of gerberas at 0, 3, and 6 days are shown in B, D, and F, with the gerberas on the left stored in water and the gerberas on the right stored in the aforementioned preservative peptide. Detailed Implementation
[0024] This invention addresses the problems of the prior art by using synthetic peptides for preserving cells, tissues and / or food and additives containing said peptides, which helps to improve the production, logistics and / or consumption of food and / or plants.
[0025] The synthetic peptides of this invention are selected from the peptides of SEQ ID NO:1 and SEQ ID NO:4 to 14, and optionally additionally include a cell-penetrating peptide tail at the N-terminal and / or C-terminal portions of the first and / or last amino acids. The peptide of SEQ ID NO:3 represents a peptide comprising the peptide of SEQ ID NO:1 and the cell-penetrating peptide of SEQ ID NO:2 in the C-terminal region. The peptide of SEQ ID NO:15 represents a peptide comprising the peptide of SEQ ID NO:9 and the cell-penetrating peptide of SEQ ID NO:2.
[0026] In one embodiment, the present invention provides a topical additive comprising the peptide and water and / or excipients for preserving cells, tissues and / or food.
[0027] In one embodiment, the present invention provides an additive for use in the hydration solution of stems or branches of flowers or inflorescences that have ornamental or phytochemical value.
[0028] In one embodiment, the peptide has a cell-penetrating peptide tail at the N-terminal and / or C-terminal portions of the first and / or last amino acids.
[0029] This invention is also defined by the following provisions.
[0030] Synthetic peptides selected from peptides of SEQ ID NO:1 and SEQ ID NO:4 to 14, for use in the preparation of preservative additives for the preservation of cells, tissues and / or food.
[0031] As described above, the peptide additionally includes a cell-penetrating peptide tail at the N-terminal and / or C-terminal portions of the first and / or last amino acids.
[0032] As described above, the cell-penetrating peptide tail has SEQ ID NO:2.
[0033] As described above, the peptide comprises SEQ ID NO:3.
[0034] As described above, the peptide comprises SEQ ID NO:15.
[0035] As described above, it is used to prepare preservative additives for plants and / or fruits with agronomic, botanical and / or medicinal value, or to increase the shelf life and safety of food.
[0036] As described above, it is used to prepare additives for regulating the germination and / or development of plants with agronomic, botanical and / or medicinal value.
[0037] As described above, it is used to prepare additives for treating flowers or inflorescences of ornamental or phytochemical value to prevent or delay wilting, rotting, or the appearance of harmful organisms (such as bacteria or fungi), and / or to maintain the quality and integrity of the flowers or inflorescences for a longer period of time.
[0038] Additives for the preservation of cells, tissues and / or food, including water and / or excipients, and synthetic peptides selected from peptides of SEQ ID NO:1 and SEQ ID NO:4 to 14.
[0039] The additive described above, wherein the peptide additionally includes a cell-penetrating peptide tail at the N-terminal and / or C-terminal portions of the first and / or last amino acids.
[0040] The additive described above, wherein the cell-penetrating peptide tail has SEQ ID NO:2.
[0041] The additive as described above, wherein the synthetic peptide has SEQ ID NO:3.
[0042] The additive as described above, wherein the peptide comprises SEQ ID NO:15.
[0043] The additives described above are used for post-harvest preservation of flowers and / or inflorescences with ornamental or phytochemical value.
[0044] The terms used in this patent application are defined as follows:
[0045] For internalization peptides to intracellular mediators In the context of this patent application, the term should be understood as any peptide that has the function of helping / allowing the internalization of a bioactive peptide into the intracellular media and thus exhibiting a biological effect in the intracellular media.
[0046] Cell cycle In the context of this patent application, the cell cycle should be understood as a series of events that occur within a cell and result in cell division and replication. It should be understood that the cell cycle includes any state of the cell cycle in microorganisms or plants, such as quiescent / senescent states and stages of interphase and cell division. Therefore, it should be understood that cell stages G0, G1, S, G2, and mitosis (M) are also part of the concept of the cell cycle.
[0047] Cell-penetrating peptide tail (CPP) In the context of this patent application, a cell-penetrating peptide tail (CPP) refers to a short amino acid sequence that facilitates the entry of the principal peptide into the intracellular medium, thereby serving as a means of peptide transport to the intracellular medium. Still within the context of this patent application, CPP also includes hydrophilic tails known in the art, such as tails TAT, SynB1, SynB3, PTD-4, PTD-5, D-Tat, R9-Tat, etc. It also includes amphiphilic tails, such as tails MAP, SBP, FBP, etc.
[0048] Cell division or proliferationIn the context of this patent application, cell division or proliferation should be understood to occur in any type of cell line, whether mammalian, plant, or microbial. Still within the context of this patent application, it should be understood that dividing cells may also be related to microbial contamination of food or plants and / or seed germination and / or plant development.
[0049] Inhibit cell proliferation In the context of this patent application, the term should be understood as inhibiting the proliferation of any type or lineage of mammalian, plant, or microbial cells, which may be at any stage of cell division and may or may not be synchronized within the cell cycle. In the context of this patent application, inhibiting cell proliferation means interrupting the cell division process, resulting in or not resulting in cell death.
[0050] Harmful substances or contaminants in plants or food In the context of this patent application, pests or contamination of plants or food are considered to be those biological agents that contaminate plants or food, are associated with the decay of plants or food, and / or cause damage to the quality or shelf life of the plant or food product. This definition includes microbial contamination that causes decay, those that form toxins, and those that alter the plant or food. Examples include, but are not limited to, *Phytophthora nicotiana* and *P. idaei*, *Botrytis cinerea*, *Colletotrichum spp. Rhizopus stolonifer*, *Sclerotiorum*, *Pestalotia longisetula Rhizoctonia sp.*, *Geotrichum sp.*, *Gnomonia comari*, (among others) *Pilidium concavum* associated with strawberry rot, *Xylella fastidiosa* in coffee and orange trees, and fungi associated with the storage, transportation, and / or pharmaceutical processing of industrial hemp.
[0051] "pep5" or "pep5" In the context of this patent application, the term should be understood to refer to the peptide represented by SEQ ID NO:1, with the amino acid sequence WELVVLGKL.
[0052] "cpp" In the context of this patent application, the term should be understood as a peptide tail, which may be represented by SEQ ID NO:2, with the amino acid sequence YGRKKRRQRRR.
[0053] pep5-cpp or Pep5-cpp or "pep5-cpp" In the context of this patent application, the term should be understood as the peptide represented by SEQ ID NO:1 linked to a peptide tail represented by sequence SEQ ID NO:2.
[0054] ΔN-pep5-cpp In the context of this patent application, the term should be understood as the peptide represented by SEQ ID NO:1, but without the last amino acid of the N-terminal portion and attached to the peptide tail represented by SEQ ID NO:2.
[0055] ΔNi-pep5-cpp In the context of this patent application, the term should be understood as the peptide represented by SEQ ID NO:1, but without the last i amino acids of the N-terminal portion, where i = 1, 2, 3, 4 or 5, and connected to the peptide tail represented by SEQ ID NO:2.
[0056] ΔC-pep5-cpp In the context of this patent application, the term should be understood as the peptide represented by SEQ ID NO:1, but without the last amino acid of the C-terminal portion and connected to the peptide tail represented by SEQ ID NO:2.
[0057] ΔCi-pep5-cpp In the context of this patent application, the term should be understood as the peptide represented by SEQ ID NO:1, but without the last i amino acids of the C-terminal portion, where i = 1, 2, 3, 4, or 5, and attached to the peptide tail represented by SEQ ID NO:2. An example is SEQ ID NO:9.
[0058] Due to minor variations in the amino acid sequences associated with SEQ ID NO:1 and SEQ ID NO:9 (both of which have been validly tested in this application specification), the peptides of SEQ ID NO:4-14 are considered to be within the inventive concept of this invention.
[0059] Polypeptide synthesis The polypeptide disclosed in this patent application was synthesized using solid-phase peptide synthesis technology (FastMocChemistr, purchased from Applied Biosystems).
[0060] The embodiments shown herein are intended to illustrate only one of many ways of carrying out the invention, and are not intended to limit its scope.
[0061] Example 1 - Preparation of plant or food preservatives using the peptides described
[0062] The peptide of SEQ ID NO:3 (which includes pep5 (SEQ ID NO:1) and cell-penetrating peptide (SEQ ID NO:2)) was prepared in a 100 μM aqueous solution for direct external application to food to be preserved.
[0063] The peptide of SEQ ID NO:15 (which includes the peptide of SEQ ID NO:9 and the cell-penetrating peptide of SEQ ID NO:2) is prepared in a 100 μM aqueous solution for use in food or plants to be preserved.
[0064] Those skilled in the art will readily understand that, in addition to water, other excipients may be incorporated into the additives of this invention to improve the preservation properties shown herein.
[0065] Example 2 - Test of an additive including the peptide in preserving strawberries from fungal contamination
[0066] The additive prepared according to Example 1 was tested for food preservation through a single external application.
[0067] Figure 1 Visual results are shown for experiments involving the application of 100 μM preservative peptide (SEQ ID NO:3) or water (control) to fresh strawberries, all from the same batch and commercial product packaging. Results for each group of 10 strawberries under each condition (experiment and control) at 0, 12, 24, 36, 48, and 60 h are shown in A, B, C, D, E, and F, respectively.
[0068] exist Figure 1 In samples D) and E), the presence of fungi with white hyphae can be observed, which are undetectable in treated strawberries. Although these contaminants have not yet been characterized, their appearance is consistent with Rhizopus stolonifer or Sclerotinia sclerotiorum.
[0069] Figure 2 The graph shows the time (horizontal axis) and percentage of spoiled fruit (vertical axis) in hours after applying 100 μM preservative peptide (SEQ ID NO:3) or water to fresh strawberries.
[0070] Figure 1 and Figure 2 The results clearly demonstrate the great potential of additives applied in aqueous solutions for fruit preservation.
[0071] Similar results were obtained by administering the peptide of SEQ ID NO:15, which includes the peptides of SEQ ID NO:9 and SEQ ID NO:2.
[0072] Example 3 - Test of additives containing the peptide in preventing strawberries from rotting
[0073] A new experiment was conducted using strawberries, and the test and control groups were now stored at 4°C (in a refrigerator) to observe the preservation under conditions unfavorable to microbial growth.
[0074] Figure 3 Visual results of experiments involving the application of the additive of the present invention are shown, wherein eight fresh strawberries in each group were treated with a 100 μM aqueous solution of the preservative peptide (SEQ ID: 3), or with water as a control only. All strawberries were from the same batch and commercial product packaging. Representative results of strawberries under each condition (experimental and control) at 0 h, 24 h, 48 h, and 5 days are shown in A, B, C, and D, respectively.
[0075] exist Figure 3 In D), the presence of severe rot in the untreated strawberry group was very evident, to the point that it could not be detected even in the strawberry group treated with the additive of the present invention after 5 days. This rot was significantly different from the microbial contamination observed in the experiment of Example 2.
[0076] Those skilled in the art will immediately understand the great technical and economic value of this invention, and can apply the additives of this invention to the preservation of other plants or foods.
[0077] Example 4—Experiment with the use of preservative additives containing the peptide in regulating the germination rate of legume seeds
[0078] To evaluate the effects of the additive of the present invention on regulating cell division or proliferation in plants (particularly in regulating seed germination and / or plant development), an experiment was conducted to observe the germination and subsequent seedling development of legume seeds. Each group of 14 black bean seeds was placed in moist cotton and subjected to one condition: a control group treated with water; and an experimental group treated with the additive of the present invention as described below. Seed status was observed and recorded at 0, 3, and 4 days, and the germination patterns and profiles of the legumes were analyzed.
[0079] Figure 4 Visual results are shown of experiments involving the application of a single dose of 100 μM (aqueous solution) of the preservative peptide (Ktut SEQ ID:3) or water (control) to legume seeds. All seeds were from the same batch and packaged in the same commercial product and stored at room temperature (28°C). Germination results of 14 seeds per group in each condition (control and experimental) at 0, 3, and 4 days are shown in A, B, and C, respectively. The images illustrate the differences in germination patterns among the legumes.
[0080] Figure 5 As shown Figure 4As shown, the time in days (horizontal axis) and the number of germinated legume seeds (vertical axis) after application of 100 μM preservative peptide (Ktut, SEQ ID NO:3) are displayed.
[0081] The results demonstrated a substantial regulation of the metabolic state of buds or seedlings upon application of the additive, as well as a delay in the seed germination process. Therefore, the present invention has proven useful for regulating germination, adjusting planting or germination time, and other agronomical or botanical advantages.
[0082] Similar results were obtained by administering the peptide of SEQ ID NO:15, which contains the peptide of SEQ ID NO:9 and the peptide of SEQ ID NO:2.
[0083] Example 5 - Experiment on the preservation of post-harvest flowers using additives containing preservative peptides
[0084] The surprising results obtained in the foregoing embodiments prompted the inventors to evaluate whether the additive of the present invention could also preserve flowers after harvest. Flowers and inflorescences are known to be particularly sensitive and prone to rapid wilting or decay, which leads to technical and economic difficulties in utilizing these plant parts. Particular emphasis is placed on the market for ornamental flowers, and especially on the market for herbs or pharmaceuticals that use flowers or inflorescences as raw materials. In these fields, there is a particular need for a method to maintain the quality and / or integrity of harvested flowers or inflorescences so as to suffer less negative impact during subsequent logistics or industrial processing.
[0085] In this context, as an experimental model for evaluating the preservation of flowers or inflorescences, two different types of flowers and their decomposition / preservation were evaluated by incubating them in water or with the preservative additive solution of the present invention (with the flowers placed at room temperature for several days).
[0086] The selected flowers are roses (Rosa-rubra) and gerberas (Gerbera). Roses are among the most cultivated ornamental flowers in the world. Gerbera species are used as experimental organisms in studies of flowering and floral meristem development, and because they contain natural coumarin derivatives, they are flowers with phytochemical and biocontrol value.
[0087] In this embodiment, the peptide of SEQ ID NO:15 is used, which comprises the peptide of SEQ ID NO:9 and the peptide of SEQ ID NO:2.
[0088] Figure 6Visual results are shown of experiments involving the application of a single dose of a 100 μM (aqueous solution) preservative peptide (SEQ ID NO: 15) or water (control group) to two types of flowers. All flowers were from the same batch and acquired on the same day, and stored at room temperature (28°C). Four flowers were used in each container, with the stems kept submerged in the liquid for at least 5 cm throughout the experiment. Aluminum foil was used around the containers containing the submerged stems to prevent light exposure. Photographic results of the flowers preserved under each condition (control and experimental) at 0, 3, and 6 days are shown. Images of roses at 0, 3, and 6 days are shown in A), C), and E), with the rose on the left preserved in H2O and the rose on the right preserved in the preservative peptide. Images of gerberas at 0, 3, and 6 days are shown in B), D), and F), with the gerberas on the left preserved in H2O and the gerberas on the right preserved in the aforementioned preservative peptide. The images clearly show a significant difference in the preservation patterns of the flowers preserved in the preservatives.
[0089] Figure 6 The results demonstrate the effective preservation of these two different flower species and provide proof of concept for the use of the additives of the present invention in ornamental flowers and inflorescences and in flowers and inflorescences with phytochemical value, such as gerberas, industrial hemp and other plant species.
[0090] Those skilled in the art will appreciate the knowledge provided herein and will be able to replicate the invention in the given embodiments and other variations covered by the scope of the appended claims. sequence list <110> PROTEIMAX BIO TECHNOLOGY ISRAEL LTD <120> Additives used for preserving cells, tissues, and / or food. <130> Proteimax‑Ktut preservante <150> BR 10 2021 002393 7 <151> 2021-02-08 <160> 15 <170> PatentIn version 3.5 <210> 1 <211> 9 <212> PRT <213> Artificial sequence <220> <223> Artificial sequence <400> 1 Trp Glu Leu Val Val Leu Gly Lys Leu 1 5 <210> 2 <211> 11 <212> PRT <213> Artificial sequence <220> <223> Artificial sequence <400> 2 Tyr Gly Arg Lys Lys Arg Arg Gln Arg Arg Arg 1 5 10 <210> 3 <211> 20 <212> PRT <213> Artificial sequence <220> <223> Artificial sequence <400> 3 Trp Glu Leu Val Val Leu Gly Lys Leu Tyr Gly Arg Lys Lys Arg Arg 1 5 10 15 Gln Arg Arg Arg 20 <210> 4 <211> 8 <212> PRT <213> Artificial sequence <220> <223> Artificial sequence <400> 4 Glu Leu Val Val Leu Gly Lys Leu 1 5 <210> 5 <211> 7 <212> PRT <213> Artificial sequence <220> <223> Artificial sequence <400> 5 Leu Val Val Leu Gly Lys Leu 1 5 <210> 6 <211> 6 <212> PRT <213> Artificial sequence <220> <223> Artificial sequence <400> 6 Val Val Leu Gly Lys Leu 1 5 <210> 7 <211> 8 <212> PRT <213> Artificial sequence <220> <223> Artificial sequence <400> 7 Trp Glu Leu Val Val Leu Gly Lys 1 5 <210> 8 <211> 7 <212> PRT <213> Artificial sequence <220> <223> Artificial sequence <400> 8 Trp Glu Leu Val Val Leu Gly 1 5 <210> 9 <211> 6 <212> PRT <213> Artificial sequence <220> <223> Artificial sequence <400> 9 Trp Glu Leu Val Val Leu 1 5 <210> 10 <211> 5 <212> PRT <213> Artificial sequence <220> <223> Artificial sequence <400> 10 Trp Glu Leu Val Val 1 5 <210> 11 <211> 6 <212> PRT <213> Artificial sequence <220> <223> Artificial sequence <400> 11 Trp Glu Leu Val Val Ala 1 5 <210> 12 <211> 6 <212> PRT <213> Artificial sequence <220> <223> Artificial sequence <400> 12 Trp Glu Ala Val Val Leu 1 5 <210> 13 <211> 6 <212> PRT <213> Artificial sequence <220> <223> Artificial sequence <400> 13 Trp Glu Ala Val Val Ala 1 5 <210> 14 <211> 6 <212> PRT <213> Artificial sequence <220> <223> Artificial sequence <220> <221> MOD_RES <222> (1)..(1) <223> acetylation <400> 14 Trp Glu Leu Val Val Leu 1 5 <210> 15 <211> 17 <212> PRT <213> Artificial sequence <220> <223> Artificial sequence <400> 15 Trp Glu Leu Val Val Leu Tyr Gly Arg Lys Lys Arg Arg Gln Arg Arg 1 5 10 15 Angry
Claims
1. A method for processing plant cells, tissues, and / or food products derived therefrom, characterized in that... It includes the application of preservative additives, said preservative additives comprising: At least one synthetic peptide containing a cell-penetrating peptide tail, The synthetic peptide is selected from SEQ ID NO: 3 and / or SEQ ID NO: 15; The method comprises the following: preventing or delaying the rotting of fruits and / or fungal contamination; delaying the germination of beans; and / or preventing or delaying the wilting of flowers for a longer period of time.
2. The method according to claim 1, characterized in that... This includes applying a preservative additive containing SEQ ID NO: 3 to prevent or delay the rotting and / or fungal contamination of strawberries.
3. The method according to claim 1, characterized in that... This includes applying a preservative containing SEQ ID NO: 3 to delay the germination of legume seeds.
4. The method according to claim 1, characterized in that... This includes applying a preservative additive containing SEQ ID NO: 15 to prevent or delay the wilting of roses and / or gerberas for a longer period of time.
5. The method according to claim 1, characterized in that... This includes the application of a preservative additive containing SEQ ID NO: 15 for post-harvest preservation of roses and / or gerberas.