Preparation method and application of double-sided tissue adhesive

Abstract

The application provides a preparation method and application of a double-sided tissue adhesive, which is based on dopamine chemistry and hydrogel coating technology, uses a xenogeneic acellular dermal matrix dressing as a substrate, and polydopamine as a coating. First, a xenogeneic acellular dermal matrix dressing with polydopamine nanoparticles deposited thereon is obtained, anisotropic xenogeneic acellular dermal matrix dressing is prepared through hydrophobic modification, and then a hydrogel coating is assembled to the hydrophilic side of the modified xenogeneic acellular dermal matrix dressing to obtain a double-layer tissue adhesive with asymmetric adhesive properties. The method for preparing the double-layer tissue adhesive has the advantages of reusability, low cost, simple operation and convenience for large-scale production, and the double-sided tissue adhesive prepared has the properties of anti-bursting, asymmetric wettability, asymmetric adhesion and anti-oxidation, can promote tissue repair and resist postoperative adhesion, and can be applied in the preparation of a dressing for promoting tissue repair.

Description

Technical Field

[0001] This invention belongs to the field of biomaterials technology, specifically relating to a method for preparing and applying a double-sided tissue adhesive, which can be used in technical fields such as promoting tissue repair. Background Technology

[0002] Hydrogels are three-dimensional polymeric networks with high water content that can promote cell migration and proliferation. In recent years, hydrogel tissue adhesives have been widely used in the biomedical field. However, due to their high water content, hydrogels often lack sufficient mechanical strength. When used on tissue surfaces under significant mechanical stress, such as in the repair of gastrointestinal injuries, they are easily damaged, thus losing their ability to protect and repair damaged tissues. Furthermore, traditional hydrogel tissue adhesives lack selective adhesion; this indiscriminate adhesion leads to unavoidable adhesion to adjacent tissues during in vivo application.

[0003] Based on dopamine chemistry and hydrogel coating techniques, we propose a double-sided tissue adhesive with high mechanical strength, high burst pressure strength, and resistance to tissue adhesion, and apply it to promote the healing of gastric perforation. Acellular dermal matrix dressings are widely used bio-dressings in clinical practice, possessing good biocompatibility, skin-like structure and strength, making them excellent skin substitutes. However, their lack of protective function limits their development and application in many clinical treatments. Dopamine is a "sticky" biomolecule containing adhesion proteins typical of mussels, which can self-polymerize into nano-coatings on various surfaces. Furthermore, this coating can provide a reaction platform for further chemical reactions, such as hydrophobic modification. In addition, hydrogel coating technology is a method of organically combining hydrogels with various substrates. By combining hydrogel coatings, the substrate can acquire various properties of hydrogels, making it an excellent approach for generating double-sided tissue adhesives.

[0004] Therefore, in this invention, we manufactured a double-sided tissue adhesive by combining dopamine chemistry and hydrogel coating technology, and studied its practical effects on tissue healing. Summary of the Invention

[0005] This invention addresses the problems existing in the prior art by providing a double-sided tissue adhesive, its preparation method, and its application, thus solving the shortcomings of traditional tissue adhesives, such as insufficient mechanical strength and easy tissue adhesion.

[0006] To achieve the above objectives, the present invention adopts the following technical solution:

[0007] (I) This invention provides a method for preparing a double-sided tissue adhesive, comprising the following steps:

[0008] S1. The decellularized dermal matrix dressing is soaked in anhydrous ethanol, then removed and placed in a hydrochloric acid dopamine solution and stirred. After removal, it is repeatedly rinsed with deionized water to remove residual polydopamine on the surface and dried.

[0009] S2. Seal one side of the decellularized dermal matrix dressing obtained in S1 to isolate it from external reactions, and perform hydrophobic modification on the other side of the decellularized dermal matrix dressing.

[0010] S3. A pre-gel solution is coated on the protected side of the decellularized dermal matrix dressing prepared in S2, and then placed in a vacuum-sealed environment. After curing, a double-sided tissue adhesive is obtained.

[0011] Furthermore, in S1, the method for preparing the dopamine hydrochloride solution is as follows: dissolve dopamine hydrochloride in Tris-HCl buffer, adjust the pH, and obtain the dopamine hydrochloride solution.

[0012] Furthermore, the concentration of the Tris-HCl buffer is 50 mmol / L, and the pH is adjusted to 8.5; the concentration of dopamine hydrochloride in the prepared dopamine hydrochloride solution is 2 mg / ml.

[0013] Furthermore, in S1, after the decellularized dermal matrix dressing is placed in the dopamine hydrochloride solution, it is vigorously stirred in a 30°C water bath for 8 hours.

[0014] Furthermore, in S1, the drying method is natural drying under ventilation at room temperature of 25°C.

[0015] Furthermore, in S2, the hydrophobic modification method is as follows: the decellularized dermal matrix dressing, which is sealed on one side, and the triethoxy-1H,1H,2H,2H-perfluorodecylsilane are placed in the same sealed container and placed in an oven at 60°C for vapor deposition for 4 hours. After vapor deposition, the mixture is cooled to room temperature and the surface is repeatedly rinsed with deionized water to remove unreacted residues.

[0016] Furthermore, in S3, the pregel solution comprises 30 w / v of acrylic acid, 5 w / v of quaternary ammonium chitosan, 3 w / v of ammonium persulfate, and 0.3 w / v of tetramethylethylenediamine.

[0017] Furthermore, in step S3, the pre-gel solution is injected onto the surface of the decellularized dermal matrix dressing using a syringe; the curing time of the pre-gel solution is 2 hours.

[0018] (ii) The present invention also provides a double-sided tissue adhesive, which is prepared by the preparation method described above.

[0019] (III) The present invention also provides the application of the double-sided tissue adhesive prepared by the above preparation method in the preparation of wound repair materials.

[0020] The beneficial effects of this invention are:

[0021] (1) This invention uses dopamine chemically modified xenogeneic decellularized dermal matrix as a base to prepare a double-sided tissue adhesive using hydrogel coating technology. The method is simple, easy to operate, inexpensive, reusable, does not require high technical requirements, and the structure and properties of the double-sided tissue adhesive are easy to control.

[0022] (2) The present invention designs a double-sided tissue adhesive that achieves asymmetric wetting and antibacterial staining properties, high mechanical strength and high burst pressure strength, and is highly practical.

[0023] (3) The double-sided tissue adhesive prepared by the present invention has good biocompatibility and good antioxidant function, and can be used to promote the repair of damaged tissue. Attached Figure Description

[0024] Figure 1 The images show a comparison of xenogeneic acellular dermal matrix before and after polydopamine coating. In Figure a, from left to right, the images are a visual image and an electron microscope image of the xenogeneic acellular dermal matrix before polydopamine coating. In Figure b, from left to right, the images are a visual image and an electron microscope image of the xenogeneic acellular dermal matrix after polydopamine coating.

[0025] Figure 2 This is a schematic diagram illustrating the stability of a polydopamine coating on xenogeneic acellular dermal matrix. In Figure a, from left to right, the polydopamine-coated xenogeneic acellular dermal matrix is ​​placed underwater, twisted into a spiral shape, and then unfolded. In Figure b, from left to right, the polydopamine-coated xenogeneic acellular dermal matrix is ​​placed underwater, and its surface is scraped with a scalpel and then scraped repeatedly three times.

[0026] Figure 3 The image shows the results of the asymmetric wettability test of the modified xenogeneic acellular dermal matrix. From top to bottom, the images show the two sides of the modified xenogeneic acellular dermal matrix and their corresponding contact angle test results.

[0027] Figure 4 Figure 1 shows the results of the antibacterial staining test of the modified xenogeneic acellular dermal matrix. Figure 2 shows the fluorescent staining results of bacterial growth and reproduction on the hydrophilic surface of the modified xenogeneic acellular dermal matrix after co-culturing with Escherichia coli for 24 hours, 48 ​​hours and 72 hours, respectively. Figure 3 shows the fluorescent staining results of bacterial growth and reproduction on the hydrophobic surface of the modified xenogeneic acellular dermal matrix after co-culturing with Escherichia coli for 24 hours, 48 ​​hours and 72 hours, respectively.

[0028] Figure 5 The diagram shows the structure of a double-sided tissue adhesive, with electron micrograph and magnified structural image of the double-sided tissue adhesive from left to right.

[0029] Figure 6 Figure 1 shows a schematic diagram of the mechanical strength of a double-sided tissue adhesive. Figure 2a is a comparison of the mechanical strength of a double-sided tissue adhesive and a hydrogel tissue adhesive; Figure 3b is a comparison of the burst pressure strength of a double-sided tissue adhesive and a hydrogel tissue adhesive.

[0030] Figure 7 The diagram shows the antioxidant properties of the double-sided tissue adhesive. From left to right, it represents the fluorescence staining results of cell survival after treatment with hydrogen peroxide, hydrogen peroxide + double-sided tissue adhesive, and normal control group.

[0031] Figure 8 This is a diagram of the anti-postoperative adhesion test of double-sided tissue adhesive. From left to right, it shows the surgical intervention of suture suturing, cyanoacrylate adhesive, hydrogel tissue adhesive and double-sided tissue adhesive on rats with gastric perforation. From top to bottom, it shows the intra-abdominal adhesion results on the day of the surgical intervention and 14 days after the operation.

[0032] Figure 9 The image shows the effect of double-sided tissue adhesive on promoting tissue repair. From left to right, the images represent the results of surgical intervention on rats with gastric perforation 14 days after the animals were euthanized and their tissue samples were collected for HE staining, including suture suturing, cyanoacrylate adhesive, hydrogel tissue adhesive, and double-sided tissue adhesive.

[0033] Figure 10 This is a schematic diagram illustrating the application of the double-sided tissue adhesive of the present invention. Detailed Implementation

[0034] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the embodiments and accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0035] Example 1: Preparation of double-sided tissue adhesive

[0036] This embodiment provides a method for preparing a double-sided tissue adhesive, including the following steps:

[0037] (1) Preparation of modified xenogeneic decellularized dermal matrix:

[0038] First, the xenogeneic decellularized dermal matrix was soaked in anhydrous ethanol.

[0039] Dilute 1 mol / L Tris-HCl buffer to 50 mmol / L with deionized water and adjust the pH of the buffer to 8.5 by adding hydrochloric acid dropwise; then dissolve dopamine hydrochloride powder at a concentration of 2 mg / ml in Tris-HCl buffer.

[0040] The xenogeneic decellularized dermal matrix, after being soaked in anhydrous ethanol, was placed in the above-mentioned hydrochloric acid dopamine solution and stirred vigorously in a 30°C water bath. After reacting for 8 hours, it was removed and repeatedly rinsed with deionized water to remove residual polydopamine on the surface. Finally, it was placed in a ventilated room at 25°C to air dry naturally.

[0041] Subsequently, one side of the naturally dried xenogeneic acellular dermal matrix dressing containing deposited polydopamine nanoparticles was sealed with tape to prevent reaction with external chemicals. The other side was left untreated and exposed. After this operation, 5 μL of triethoxy-1H,1H,2H,2H-perfluorodecylsilane was uniformly dispersed in a 2 mL dichloromethane solution, and the solution and the sealed xenogeneic acellular dermal matrix dressing were placed in a sealed plastic box. The plastic box was then placed in a 60°C oven for vapor deposition. After 4 hours, the xenogeneic acellular dermal matrix dressing was removed and its surface was washed with deionized water.

[0042] like Figure 1 As shown, Figure 1 This is a comparison image of polydopamine coating on xenogeneic decellularized dermal matrix before and after treatment. Figure 1 From left to right, images a) are a visual representation and an electron microscope image of xenogeneic decellularized dermal matrix before polydopamine coating. Figure 1 b. From left to right: a direct visual image and an electron microscope image of the xenogeneic decellularized dermal matrix coated with polydopamine. Figure 1 As can be seen, the surface of the obtained xenogeneic decellularized dermal matrix dressing is black, with a large number of polydopamine nanoparticles deposited on it.

[0043] Figure 2 This image shows the results of a stability test of a polydopamine coating on a xenogeneic decellularized dermal matrix. Figure 2 From left to right, image a shows a decellularized xenogeneic dermal matrix coated with polydopamine placed underwater, twisted into a spiral shape, and then unfolded. The water color remained unchanged during this process, proving that the polydopamine coating did not peel off. Additionally... Figure 2From left to right, step b shows a polydopamine-coated acellular dermal matrix placed underwater. The surface was then scraped off with a scalpel, repeated three times. During this process, the water color remained unchanged, and no polydopamine nanoparticles remained on the scalpel, demonstrating the extremely high stability of the polydopamine coating. Therefore, from... Figure 2 It can be seen that the polydopamine coating prepared in this embodiment has good stability and is not easily damaged by external forces.

[0044] (2) Preparation of hydrogel tissue adhesive:

[0045] Prepare a 30% (w / v) acrylic acid solution by dissolving quaternary ammonium chitosan powder at a concentration of 5% (w / v) in the acrylic acid solution. Stir vigorously at room temperature for 3 hours to form a clear and transparent mixed solution. Then, add ammonium persulfate powder at a concentration of 3% (w / v) and tetramethylethylenediamine at a concentration of 0.3% (w / v) to the above mixed solution. Stir vigorously at room temperature for 5 minutes to form a clear and transparent mixed solution.

[0046] (3) Preparation of double-sided tissue adhesive:

[0047] The hydrogel solution prepared in step (2) was drawn using a syringe and slowly and evenly injected onto the protected side of the modified xenogeneic acellular dermal matrix dressing prepared in step (1). The pre-gel solution and the modified xenogeneic acellular dermal matrix dressing prepared in step (1) were then placed together in a vacuum environment. After 2 hours, the hydrogel naturally solidified and bonded tightly with the modified xenogeneic acellular dermal matrix dressing prepared in step (1), forming a double-sided structure.

[0048] like Figure 5 As shown, from left to right, are an electron microscope image and a magnified structural image of the double-sided tissue adhesive. It can be seen that the obtained tissue adhesive has two different sides, and no gaps were observed between the two sides; they are tightly linked together to form a whole.

[0049] The double-sided tissue adhesive obtained in this embodiment combines the characteristics of hydrogels and xenogeneic acellular dermal matrix dressings. It integrates the asymmetric wetting and antibacterial staining properties of modified xenogeneic acellular dermal matrix dressings, and exhibits skin-like high mechanical strength and high burst pressure strength, antioxidant properties, anti-tissue adhesion, and tissue repair promotion effects.

[0050] Example 2: Physical and biological effects of double-sided tissue adhesives

[0051] (1) Asymmetric wettability of modified xenogeneic decellularized dermal matrix

[0052] The modified xenogeneic acellular dermal matrix prepared in Example 1(1) was placed underwater and then removed to observe the wettability of both sides of the modified xenogeneic acellular dermal matrix. Then, the modified xenogeneic acellular dermal matrix was placed on a contact angle measuring instrument to detect the contact angles on both sides.

[0053] The results are as follows Figure 3 As shown, Figure 3 The images show the asymmetric wettability of the modified xenogeneic acellular dermal matrix. From top to bottom, they are visual representations of both sides of the modified xenogeneic acellular dermal matrix and the corresponding contact angle test results. It is evident that the two sides of the modified xenogeneic acellular dermal matrix exhibit diametrically opposed hydrophilic and hydrophobic properties, demonstrating asymmetric wettability.

[0054] (2) Antibacterial and antistaining properties of modified xenogeneic acellular dermal matrix

[0055] According to McFarland standards, resuscitated *E. coli* (ATCC 25922) was diluted to 0.5 McFarland turbidity with sterile PBS. It was then cultured in Luria-Bertani (LB) liquid medium. Simultaneously, the modified xenogeneic acellular dermal matrix prepared in Example 1 (1) was placed in the liquid medium. The modified xenogeneic acellular dermal matrix was removed at 24, 48, and 72 hours after co-culturing with the bacteria, and the bacteria were stained with SYTO 9 green fluorescent nucleic acid dye.

[0056] The results are as follows Figure 4 As shown, Figure 4 This image shows the antibacterial and antistaining properties of modified xenogeneic decellularized dermal matrix. Figure 4 a. From left to right, these are the fluorescent staining results of bacterial growth and reproduction on the hydrophilic surface of the modified xenogeneic decellularized dermal matrix after co-culturing with Escherichia coli for 24 hours, 48 ​​hours, and 72 hours, respectively. Figure 4 b, from left to right, shows the fluorescent staining results of bacterial growth and reproduction on the hydrophobic surface of the modified xenogeneic acellular dermal matrix after co-culturing with *E. coli* for 24 hours, 48 ​​hours, and 72 hours, respectively. It can be seen that the hydrophobic surface of the modified xenogeneic acellular dermal matrix possesses antibacterial and antifouling properties.

[0057] (3) Mechanical strength of double-sided adhesive

[0058] The hydrogel tissue adhesive prepared in Example 1(2) and the double-sided tissue adhesive prepared in Example 1(3) were subjected to tensile fracture tests on a universal mechanical testing machine (CMT2103).

[0059] Experimental results are as follows Figure 6 As shown in a, Figure 6a represents a comparison of the mechanical strength of double-sided tissue adhesives and hydrogel tissue adhesives. Compared with simple hydrogel tissue adhesives, the mechanical strength of double-sided tissue adhesives is significantly improved, reaching 0.7 MPa, which is close to the strength of skin tissue.

[0060] (4) Bursting compressive strength of double-sided adhesive

[0061] The hydrogel tissue adhesive prepared in Example 1(2) and the double-sided tissue adhesive prepared in Example 1(3) were adhered to the surface of an isolated pig stomach with a rupture. Air was then injected into the pig stomach through one end (cardiac sphincter), and a pressure testing instrument was connected to the other end (pylorus). The burst pressure displayed by the pressure testing instrument when the rupture in the pig stomach was ruptured was the burst pressure strength of the tested material.

[0062] Experimental results are as follows Figure 6 As shown in b. Figure 6 b shows a comparison of the burst pressure strength of the double-sided tissue adhesive and the hydrogel tissue adhesive. Compared with the hydrogel tissue adhesive alone, the burst pressure strength of the double-sided tissue adhesive is significantly improved, reaching 24 kPa.

[0063] (5) Antioxidant properties of double-sided adhesive

[0064] The double-sided tissue adhesive prepared in Example 1(3) was placed in a 24-well plate and soaked in 2 mL of DMEM medium for 24 hours. Additionally, a density of 5 × 10⁻⁶ was used. 5 L929 fibroblasts were cultured in 1 mL DMEM at a concentration of 1 cell / well in 24-well plates for 24 hours. Hydrogen peroxide was added to the culture medium to prepare a 0.1 mmol / L hydrogen peroxide solution. This 0.1 mmol / L hydrogen peroxide solution was used to replace the culture medium in the 24-well plates. Cell counts were determined using a fluorescence microscope after 24 hours. The experimental results are shown below. Figure 7 As shown.

[0065] Figure 7 This image shows the antioxidant effect of the double-sided tissue adhesive. From left to right, the images depict the fluorescence staining results of cell survival after treatment with hydrogen peroxide, hydrogen peroxide + double-sided tissue adhesive, and the normal control group. Hydrogen peroxide treatment resulted in significant cell death, while treatment with the double-sided tissue adhesive significantly increased the number of surviving cells, approaching the level of the normal cell culture control group. This confirms that the double-sided tissue adhesive possesses good antioxidant properties.

[0066] Example 3: The anti-postoperative adhesion and tissue repair effects of double-sided tissue adhesive

[0067] (1) Animal modeling

[0068] Rats were anesthetized by intraperitoneal injection of Sertazone 50 (40 mg / kg), and then the abdominal skin and muscles were incised along the midline of the abdomen with a scalpel to open the abdominal cavity. A 0.5 cm full-thickness incision was made at the greater curvature of the rat's stomach with a scalpel.

[0069] (2) Injury intervention

[0070] Twenty male SD rats, weighing 180–200 g, were randomly divided into four groups. The model was established as described in Example 3(1). Subsequently, sutures were applied, followed by cyanoacrylate adhesive, hydrogel tissue adhesive prepared in Example 1(2), and double-sided tissue adhesive prepared in Example 1(3), respectively. The abdominal cavity was then closed by interrupted sutures. Specimens were observed and stained with hematoxylin and eosin (HE) on day 14. The experimental results are shown in […]. Figure 8 and Figure 9 .

[0071] Figure 8 This image shows the results of a postoperative adhesion test using a double-sided tissue adhesive. From left to right, the results represent surgical interventions in rats with gastric perforation using sutures, cyanoacrylate adhesives, hydrogel tissue adhesives, and double-sided tissue adhesives. From top to bottom, the results show intra-abdominal adhesions on the day of surgery and 14 days post-surgery. Figure 9 The effect of double-sided tissue adhesive on promoting tissue repair: From left to right, the results are shown for rats with gastric perforation who underwent surgical intervention 14 days after surgery, with the animals euthanized and tissue samples collected for HE staining. The double-sided tissue adhesive effectively repaired gastric perforation damage and prevented postoperative adhesions. Figure 8 and Figure 9 It can be seen that the double-sided tissue adhesive prepared in this invention can repair gastric perforation damage and prevent postoperative adhesions.

[0072] The double-sided tissue adhesive prepared by this invention combines the characteristics of hydrogel and xenogeneic acellular dermal matrix dressing. It integrates the asymmetric wetting and antibacterial staining properties of modified xenogeneic acellular dermal matrix dressing, and exhibits skin-like high mechanical strength and high burst pressure resistance. It also has good antioxidant properties, anti-tissue adhesion and tissue repair promotion effects.

[0073] This invention discloses the preparation and application of a double-sided hydrogel adhesive. The method is based on dopamine chemistry and hydrogel coating technology. Using a xenogeneic decellularized dermal matrix dressing as a substrate and polydopamine as a coating, a xenogeneic decellularized dermal matrix dressing with polydopamine nanoparticle deposition is first obtained. The polydopamine nanoparticles on the surface of the xenogeneic decellularized dermal matrix dressing are then selectively hydrophobically modified with triethoxy-1H,1H,2H,2H-perfluorodecylsilane to prepare an anisotropic xenogeneic decellularized dermal matrix dressing. The hydrogel coating is then assembled onto the hydrophilic side of the modified xenogeneic decellularized dermal matrix dressing (the other side being the hydrophobic side) to obtain a double-layer tissue adhesive with asymmetric adhesion properties. The xenogeneic acellular dermal matrix dressing prepared in this invention achieves anisotropic wettability through dopamine chemistry. The resulting tissue adhesive, combined with a hydrogel coating, absorbs the characteristics and advantages of both the acellular dermal matrix dressing and the hydrogel, demonstrating potential value in tissue repair. This method for preparing a bilayer tissue adhesive based on dopamine chemistry and hydrogel coating technology offers advantages such as reusability, low cost, ease of operation, and suitability for large-scale production. The prepared bilayer tissue adhesive exhibits burst resistance, asymmetric wettability, asymmetric adhesion, and antioxidant properties, promoting tissue repair and preventing postoperative adhesions, making it applicable in the preparation of dressings that promote tissue repair.

[0074] The above are merely preferred embodiments of the present invention. The scope of protection of the present invention is not limited to the above embodiments. All technical solutions falling within the scope of the present invention's concept are within the scope of protection of the present invention. It should be noted that for those skilled in the art, any improvements and modifications made without departing from the principles of the present invention should be considered within the scope of protection of the present invention.

Claims

1. A method for preparing a double-sided adhesive, characterized in that, Includes the following steps: S1. Soak the decellularized dermal matrix dressing in anhydrous ethanol, remove it, then place it in a dopamine hydrochloride solution and stir. After removing it, wash it with deionized water and dry it. S2. Seal and protect one side of the decellularized dermal matrix dressing obtained in S1, and perform hydrophobic modification on the other side of the decellularized dermal matrix dressing. S3. Coat the protected side of the decellularized dermal matrix dressing prepared in S2 with a pregel solution, and then place it in a vacuum-sealed environment. After curing, a double-sided tissue adhesive is obtained. In S2, the hydrophobic modification method is as follows: the decellularized dermal matrix dressing, sealed on one side, and triethoxy-1H,1H,2H,2H-perfluorodecylsilane are placed in the same sealed container and placed in an oven at 60°C for vapor deposition for 4 hours. After vapor deposition, the mixture is cooled to room temperature and the surface is repeatedly rinsed with deionized water to remove unreacted residues. In S3, the pregel solution includes 30 w / v acrylic acid, 5 w / v quaternary ammonium chitosan, 3 w / v ammonium persulfate, and 0.3 w / v tetramethylethylenediamine.

2. The method for preparing the double-sided adhesive according to claim 1, characterized in that, In S1, the preparation method of dopamine hydrochloride solution is as follows: dissolve dopamine hydrochloride in Tris-HCl buffer, adjust the pH, and obtain dopamine hydrochloride solution.

3. The method for preparing the double-sided adhesive according to claim 2, characterized in that, The Tris-HCl buffer solution was prepared with a concentration of 50 mmol / L and the pH was adjusted to 8.

5. The concentration of dopamine hydrochloride in the prepared dopamine hydrochloride solution was 2 mg / ml.

4. The method for preparing the double-sided adhesive according to claim 1, characterized in that, In step S1, the decellularized dermal matrix dressing is placed in a dopamine hydrochloride solution and then vigorously stirred in a 30°C water bath for 8 hours.

5. The method for preparing the double-sided adhesive according to claim 1, characterized in that, In S1, the drying method is natural drying under ventilation at room temperature of 25°C.

6. The method for preparing the double-sided adhesive according to claim 1, characterized in that, In step S3, the pre-gel solution is injected onto the surface of the decellularized dermal matrix dressing using a syringe. The curing time for the pregel solution is 2 hours.

7. The double-sided tissue adhesive prepared by the preparation method according to any one of claims 1 to 6.

8. The application of the double-sided tissue adhesive prepared by the preparation method according to any one of claims 1 to 6 in the preparation of wound repair materials.

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