Traditional Chinese medicine composition and oral liquid for preventing and treating chicken synoviae mycoplasma disease, and preparation method and application thereof

Abstract

The invention provides a Chinese medicine composition, an oral liquid, a preparation method and an application thereof for preventing and treating chicken synoviae mycoplasma disease, and belongs to the field of veterinary products. The active ingredients thereof include the following composition in parts by weight: 200-600 parts of Caulis Sinomenii, 200-600 parts of Spatholobi Caulis, and 20-600 parts of Cyathula officinalis. The prescription cost of the invention is low, and the prescription synergistic advantage is provided. The prevention and treatment of chicken synoviae mycoplasma disease has obvious effect, the number of prescription flavors is simplified, and the preparation is convenient, so as to improve the cost performance of preventing and treating chicken synoviae mycoplasma disease and improve the economic benefit.

Description

Technical Field

[0001] The invention relates to the technical field of veterinary drugs, and in particular to a traditional Chinese medicine composition for preventing and treating chicken synoviae mycoplasma disease, a traditional Chinese medicine composition oral liquid, and a preparation method and application thereof. Background Art

[0002] Mycoplasma Synoviae (MS) can cause respiratory diseases, infectious synovitis, abnormal eggshell tops, and growth retardation in poultry, and manifest as arthritis symptoms, affecting feeding and drinking, and can cause body weakness and death in severe cases; it is mainly characterized by exudative arthritis, tenosynovitis, and bursitis, which affect egg production: because of substandard weight and uniformity, the start of laying and peak egg production in chickens are delayed by 7 to 15 days, and the impact on the overall egg production rate is about 5%. It brings huge economic losses to the poultry industry. The pathogen has no cell wall and only one serotype. It has strict requirements for isolation and culture. Its genome is smaller than that of bacteria, and it has a strong antigenic variation ability. It can be transmitted horizontally and vertically, mainly through the respiratory tract, direct contact, and blood. MS adheres to host cells through membrane proteins, releases toxic products, evades immunity, and plunders nutrients, thereby causing damage or death to host cells. The inactivated MS vaccine is safe and has a high protection rate. It can prevent the disease from colonizing in the air sac and trachea, while the live vaccine cannot protect the infected chickens. After vaccination, it will induce the disease or aggravate the clinical symptoms. The infection rate of MS in China is increasing year by year. According to reports, the overall average serum positive rate of chickens in some areas of Jiangsu is 57.7%, and the highest can reach 82.4%. The economic losses caused to domestic chicken farming companies are also increasing year by year.

[0003] Traditional Chinese medicine is widely used in veterinary medicine and has irreplaceable advantages in certain aspects. It has unique advantages such as being less likely to develop drug resistance and treating both the symptoms and the root causes. Traditional Chinese veterinary medicine believes that the lesions of Mycoplasma synoviae are mainly caused by "cold and dampness", which can easily lead to blockage of tendons, muscles, and joints, and blockage of meridians, affecting the circulation of qi, blood, and body fluids. It is a kind of arthritis and should be treated with traditional Chinese medicine that can promote blood circulation, remove blood stasis, warm the meridians and dredge the collaterals.

[0004] However, the existing Chinese herbal medicine formulas for treating arthritis and paralysis, such as Coptis chinensis, Atractylodes macrocephala, Gentiana macrophylla, Stephania tetrandra, etc., are expensive, costing hundreds or even thousands of yuan per kilogram, which greatly increases the maintenance costs for farmers.

[0005] The development of a Chinese veterinary medicine that is effective, safe, and inexpensive has become an urgent market demand at present and for a long time to come. Summary of the invention

[0006] Purpose of the Invention

[0007] The object of the present invention is to provide a Chinese medicine composition for preventing and treating chicken synoviae mycoplasma disease, a Chinese medicine composition oral liquid, and a preparation method and application thereof. The present invention provides a pure Chinese medicine preparation with obvious effect in preventing and treating chicken synoviae mycoplasma disease by analyzing the symptoms of chicken synoviae mycoplasma and screening and optimizing the formula. The formula has synergistic advantages, obvious clinical effects, simplified formula ingredients, and convenient preparation, thereby improving the cost-effectiveness of preventing and treating chicken synoviae mycoplasma disease and improving economic benefits.

[0008] Solution

[0009] To achieve the purpose of the present invention, the technical solution adopted by the present invention is as follows:

[0010] In a first aspect, the present invention provides a traditional Chinese medicine composition for preventing and treating chicken synovia mycoplasma disease, wherein the active ingredients include the following composition in parts by weight: 200-600 parts of Caulis Sinomenii, 200-600 parts of Millettia reticulata, and 20-600 parts of Cyathula capitata.

[0011] Furthermore, the active ingredients include the following composition in parts by weight: 200-600 parts of Sinomenium spatholobi, 200-600 parts of Millettia reticulata, and 20-60 parts of Cyathula capitata.

[0012] Furthermore, the active ingredients include the following composition in parts by weight: 200-400 parts of Sinomenium spatholobi, 200-400 parts of Millettia reticulata, and 30-50 parts of Cyathula capitata.

[0013] Furthermore, the active ingredients include the following composition in parts by weight: 300 parts of Sinomenium spatholobi, 300 parts of Millettia reticulata, and 30-50 parts of Cyathula capitata.

[0014] Furthermore, the active ingredients include the following composition in parts by weight: 300 parts of Caulis Sinomenii, 300 parts of Millettia Spatholobi, and 40 parts of Cyathula officinalis.

[0015] Furthermore, the active ingredients include the following composition in parts by weight: 300 parts of Caulis Sinomenii, 300 parts of Millettia Spatholobi, and 30 parts of Cyathula officinalis.

[0016] Furthermore, the active ingredients include the following composition in parts by weight: 300 parts of Caulis Sinomenii, 300 parts of Millettia Spatholobi, and 50 parts of Cyathula officinalis.

[0017] Furthermore, the dosage form of the Chinese medicine composition is any one of oral liquid, powder, spray, paste, injection, and capsule; optionally, it is an oral liquid.

[0018] Furthermore, the amount of crude drug in the oral solution is 0.5-5 g / mL, optionally 0.8-2 g / mL, optionally 1-2 g / mL.

[0019] In a second aspect, a Chinese medicine composition oral liquid is provided, which is prepared according to the Chinese medicine composition described in the first aspect.

[0020] In a third aspect, a method for preparing an oral liquid of the Chinese medicine composition described in the first aspect or the Chinese medicine composition described in the second aspect is provided, comprising: mixing Caulis Sinomenii, Millettia reticulata, and Cyathula capitata in proportion, performing hot extraction and water extraction to obtain a concentrated liquid.

[0021] Furthermore, the hot extraction and water extraction method is: the extraction temperature is 70-100°C.

[0022] Furthermore, add 5 to 15 times the amount of water, decoct 1 to 3 times, and combine.

[0023] In a fourth aspect, a chicken feed is provided, comprising a basic chicken feed and also comprising the Chinese medicine composition described in the first aspect, or the oral liquid of the Chinese medicine composition described in the second aspect, or a product prepared by the preparation method described in the third aspect.

[0024] In a fifth aspect, a use of the Chinese medicine composition described in the first aspect, or the Chinese medicine composition powder described in the second aspect, or the product prepared by the preparation method described in the third aspect, or the chicken feed described in the fourth aspect in the preparation of a product for preventing and treating chicken synovia bursa mycoplasma disease is provided.

[0025] Beneficial Effects

[0026] The invention provides a pure traditional Chinese medicine preparation with obvious effect in preventing and treating Mycoplasma synoviae by analyzing the symptoms of Mycoplasma synoviae and by screening and optimizing the prescription. The prescription has synergistic advantages, obvious clinical effects and simple preparation, and improves the cost performance of preventing and treating Mycoplasma synoviae disease in chickens and improves the economic benefits. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] One or more embodiments are exemplarily described by the pictures in the accompanying drawings, and these exemplary descriptions do not constitute limitations on the embodiments. The special word "exemplary" here means "used as an example, embodiment or illustrative". Any embodiment described as "exemplary" here is not necessarily interpreted as being superior or better than other embodiments.

[0028] Figure 1 The photos of the chicken joints used for judging the lesion scores in the test example 1 of the present invention. Among them, A is a photo of the tarsal joint of a normal chicken; B is a photo of the tarsal joint of an affected chicken, which is evaluated as 1 point; C is a photo of the tarsal joint of an affected chicken, which is evaluated as 2 points; D is a photo of the tarsal joint of an affected chicken, which is evaluated as 3 points.

[0029] Figure 2 The PCR test results of the sample submitted for test example 2, where the channels are: M: Marker

[0030] 1: NDV results 2: NDV positive control 3: H9 results 4: H9 positive control

[0031] 5: IBV results 6: IBV positive control 7: MG results 8: MG positive control

[0032] 9: MS results 10: MS positive control 11: FAV results 12: FAV positive control

[0033] 13: A1 results 14: A1 positive control 15: A2 results 16: A2 positive control

[0034] Figure 3 This is a post-mortem examination diagram of a diseased chicken in Test Example 2 of the present invention.

[0035] Figure 4 This is a diagram of the status of the chicken flock before and after the medication of Test Example 2 of the present invention, wherein A is a diagram of the status of the chicken flock in the 7th chicken house before the medication; and B is a diagram of the status of the chicken flock in the 7th chicken house after the medication is completed. DETAILED DESCRIPTION

[0036] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention will be described clearly and completely below. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0037] In addition, in order to better illustrate the present invention, numerous specific details are provided in the specific embodiments below. It should be understood by those skilled in the art that the present invention can also be implemented without certain specific details. In some embodiments, raw materials, elements, methods, means, etc. well known to those skilled in the art are not described in detail, so as to highlight the main purpose of the present invention.

[0038] The pure Chinese medicine used to prevent and treat chicken synoviae mycoplasma disease is composed of: Caulis Sinomenii, Millettia reticulata, Cyathula officinalis, among which:

[0039] Sinomenium acuturn: It is the dried vine stems of Sinomenium acuturn (Thunb.) Rehd. et Wils. and Sinamenium acutum (Thunb.) Rehd. et Wils. var. cinereum Rehd. et Wils. of the Menispermaceae family. It is bitter, pungent and neutral in nature. It enters the liver and spleen meridians, has the effects of removing rheumatism, unblocking meridians, and promoting urination. It is mainly used to treat rheumatic arthritis and edema and beriberi.

[0040] Millettia reticulata: It is the dried vine stem of Spatholobus suberectus Dunn, a plant of the Leguminosae family. It has the effects of promoting blood circulation and replenishing blood, regulating menstruation and relieving pain, and relaxing muscles and activating collaterals. It is also effective for rheumatic pain, numbness and paralysis, blood deficiency and chlorosis, irregular menstruation, dysmenorrhea, amenorrhea and gynecological diseases. It is bitter, sweet and warm in nature. It has the effects of promoting blood circulation and replenishing blood, regulating menstruation and relieving pain, and relaxing muscles and activating blood circulation. It is mainly used to treat irregular menstruation, dysmenorrhea, amenorrhea; rheumatic pain, numbness and paralysis, blood deficiency and chlorosis.

[0041] Cyathula officinalis Kuan, a Chinese medicine. It is the dried root of Cyathula officinalis Kuan, a plant of the Amaranthaceae family. It tastes sweet and slightly bitter, and is neutral in nature. It has the effects of removing blood stasis and promoting menstruation, promoting joint function, promoting diuresis and relieving stranguria. It is mainly used for symptoms such as amenorrhea, retained placenta, traumatic injury, rheumatic arthralgia, foot paralysis and muscle spasm, hematuria and stranguria.

[0042] Test Example 1 Prescription Screening Test

[0043] Formula preparation

[0044] According to the dialectical analysis of traditional Chinese veterinary medicine, chicken synoviae Mycoplasma belongs to arthralgia. According to the theory of traditional Chinese medicine, it is advisable to use Chinese medicine that can promote blood circulation and remove blood stasis, warm the meridians and dredge the collaterals. Select low-priced Chinese medicine from the Chinese medicines that can remove rheumatism and promote blood circulation and remove blood stasis, and use the orthogonal test method to screen the best formula. The orthogonal test grouping is shown in Table 1.

[0045] Table 1. Orthogonal test grouping table

[0046] Grouping Sichuan Achyranthes Ivy Motherwort Millettia reticulata Zeeland Sea breeze vine Vaccaria Group 1 1 1 1 1 1 1 1 Group 2 1 1 1 2 2 2 2 Group 3 1 2 2 1 1 2 2 Group 4 1 2 2 2 2 1 1 Group 5 2 1 2 1 2 1 2 Group 6 2 1 2 2 1 2 1 Group 7 2 1 1 2 2 2 1 Group 8 2 2 1 2 1 1 2

[0047] Note: In Table 1, "1" represents the choice of using this drug; "2" represents the choice of not using this drug.

[0048] The preparation method of each group of prescriptions is:

[0049] Group 1: Take equal parts by weight of Cyathula officinalis, Caulis Sinomenii, Leonurus japonicus, Millettia reticulata, Eupatorium fortunei, Caulis Piperis damispheres and Vaccaria segetalis, add 10 times the amount of water, decoct twice, each time for 1.5 hours, combine the filtrates and concentrate to contain 1 g / mL of crude drug.

[0050] Group 2: Take the same weight proportions of Cyathula officinalis, Caulis Sinomenii and Leonurus japonicus, add 10 times the amount of water, decoct twice, each time for 1.5 hours, combine the filtrates, and concentrate to contain 1 g / mL of crude drug.

[0051] Group 3: Take the same weight proportions of Cyathula officinalis, Millettia reticulata, and Eupatorium fortunei, add 10 times the amount of water, decoct twice, each time for 1.5 hours, combine the filtrates, and concentrate to contain 1 g / mL of crude drug.

[0052] Group 4: Take the same weight proportions of Cyathula capitata, Caulis Piperis ternata and Vaccaria segetalis, add 10 times the amount of water, decoct twice, each time for 1.5 hours, combine the filtrates and concentrate to contain 1 g / mL of crude drug.

[0053] Group 5: Take the same weight proportions of Caulis Sinomenii, Millettia Spatholobi and Caulis Piperis Kadsurii, add 10 times the amount of water, decoct twice, each time for 1.5 hours, combine the filtrates, and concentrate to contain 1 g / mL of crude drug.

[0054] Group 6: Take the same weight proportions of Sinomenium sinensis, Eupatorium fortunei and Vaccaria segetalis, add 10 times the amount of water, decoct twice, each time for 1.5 hours, combine the filtrates, and concentrate to contain 1 g / mL of crude drug.

[0055] Group 7: Take the same weight proportions of Sinomenium sinensis, Leonurus japonicus and Vaccaria segetalis, add 10 times the amount of water, decoct twice, each time for 1.5 hours, combine the filtrates, and concentrate to contain 1 g / mL of crude drug.

[0056] Group 8: Take the same weight proportions of Leonurus japonicus, Eupatorium fortunei and Caulis damicornis, add 10 times the amount of water, decoct twice, each time for 1.5 hours, combine the filtrates, and concentrate to contain 1 g / mL of crude drug.

[0057] Formula screening

[0058] Experimental animals: 36-60 day old SPF chickens were purchased from Beijing Boehringer Ingelheim Weitong Biotechnology Co., Ltd.

[0059] Culture medium: Modified Frey's medium, porcine serum.

[0060] Grouping: SPF chickens were divided into 10 groups, each with 20 SPF chickens. The 10 groups were: blank control group, model group, and drug groups 1 to 8 (used to be gavaged with 0.5 mL of the drug solution of groups 1 to 8 above). The chickens in the model group and drug groups 1 to 8 were first challenged with poison before the experiment. Five days after the challenge, drug groups 1 to 8 were gavaged with 0.5 mL of the drug solution of groups 1 to 8 above. The drug was given continuously for 7 days. The lesions were observed on the second day of the last administration, and the lesions of each group were scored. The blank group and the model group were gavaged with the same dose of water.

[0061] The chickens in the model group and drug groups 1 to 8 were challenged with toxic substances before the experiment: 36-60-day-old SPF chickens were selected and infected with fresh bacterial liquid (1×10 9 CCU / mL)0.3mL. Observe joint lesions after infection. The tarsal joints and surrounding muscles of the infected chickens are swollen, the swollen part has obvious elastic texture, and there is a large amount of synovial fluid in the joint cavity. In seriously infected chickens, the swelling extends to the soles of the legs or feet, and the chickens lie on the ground and cannot get up.

[0062] Lesion assessment and scoring method:

[0063] 0 points: No visible changes in the joint at the injection site (see Figure 1 A).

[0064] 1 point: Slight swelling at the injection site (see Figure 1 B).

[0065] 2 points: The injection site is swollen and elastic, and there is a significant amount of synovial fluid in the joint cavity (see Figure 1 C).

[0066] 3 points: Severe swelling at the inoculation site, with a large amount of synovial fluid in the joint cavity, and the chicken cannot get up (see Figure 1 D).

[0067] Evaluation of drug effects, lesion scoring:

[0068] The lesion scores of each chicken in each group were added together and divided by the total number of chickens to calculate the lesion score for each group.

[0069] The average score of the blank group was 0, and the score of the model group was 2.34, indicating that the model was successfully established. Drug groups 1 to 8 were administered for 7 consecutive days. The lesions were observed on the second day of the last administration, and the lesion scores of each group were determined. The results are shown in Table 2.

[0070] Table 2. Average lesion scores of drug groups 1 to 8 and orthogonal test analysis results

[0071]

[0072] Note: In Groups 1 to 8 in Table 2, "1" represents the choice of using this drug; "2" represents the choice of not using this drug.

[0073] As can be seen from Table 2, Caulis Sinomenii, Cyathula capitata and Millettia reticulata have more obvious effects in the prescription, and the effect size is Millettia reticulata > Caulis Sinomenii > Cyathula capitata, so the prescription selected is Caulis Sinomenii, Cyathula capitata and Millettia reticulata.

[0074] In order to further test the therapeutic effects of Caulis Sinomenii, Cyathula capitata, and Millettia reticulata, different formulas were obtained by adjusting different ratios. Some of the formulas are as follows:

[0075] Example 1

[0076] Take 30 parts of Caulis Sinomenii, 300 parts of Millettia Spatholobi, and 300 parts of Cyathula officinalis in weight, add 10 times the amount of water, decoct twice, each time for 1.5 hours, combine the filtrate, and concentrate to contain 1 g / mL of crude drug.

[0077] Example 2

[0078] Take 300 parts of Caulis Sinomenii, 30 parts of Millettia Spatholobi, and 300 parts of Cyathula officinalis in weight, add 10 times the amount of water, decoct twice, each time for 1.5 hours, combine the filtrate, and concentrate to contain 1 g / mL of crude drug.

[0079] Example 3

[0080] Take 300 parts of Caulis Sinomenii, 300 parts of Millettia Spatholobi, and 30 parts of Cyathula capitata in weight, add 10 times the amount of water, decoct twice, each time for 1.5 hours, combine the filtrate, and concentrate to contain 1 g / mL of crude drug.

[0081] Example 4

[0082] Take 300 parts of Caulis Sinomenii, 300 parts of Millettia Spatholobi, and 300 parts of Cyathula officinalis in weight, add 10 times the amount of water, decoct twice, each time for 1.5 hours, combine the filtrate, and concentrate to contain 1 g / mL of crude drug.

[0083] Example 5

[0084] Take 300 parts of Caulis Sinomenii, 300 parts of Millettia Spatholobi, and 20 parts of Cyathula capitata in weight, add 10 times the amount of water, decoct twice, each time for 1.5 hours, combine the filtrate, and concentrate to contain 1 g / mL of crude drug.

[0085] Example 6

[0086] Take 300 parts of Caulis Sinomenii, 300 parts of Millettia Spatholobi, and 30 parts of Cyathula capitata in weight, add 10 times the amount of water, decoct twice, each time for 1.5 hours, combine the filtrate, and concentrate to contain 1 g / mL of crude drug.

[0087] Example 7

[0088] Take 300 parts of Caulis Sinomenii, 300 parts of Millettia Spatholobi, and 40 parts of Cyathula officinalis in weight, add 10 times the amount of water, decoct twice, each time for 1.5 hours, combine the filtrate, and concentrate to contain 1 g / mL of crude drug.

[0089] Example 8

[0090] Take 300 parts of Caulis Sinomenii, 300 parts of Millettia Spatholobi, and 50 parts of Cyathula capitata in weight, add 10 times the amount of water, decoct twice, each time for 1.5 hours, combine the filtrate, and concentrate to contain 1 g / mL of crude drug.

[0091] Comparative Example 1

[0092] Take 300 parts of cinnamon twig, 300 parts of Millettia reticulata, and 50 parts of Cyathula capitata in weight, add 10 times the amount of water, decoct twice, each time for 1.5 hours, combine the filtrate, and concentrate to contain 1 g / mL of crude drug.

[0093] Comparative Example 2

[0094] Take 300 parts of Caulis Sinomenii, 300 parts of Caulis Sargentodoxae, and 50 parts of Cyathula capitata in weight, add 10 times the amount of water, decoct twice, each time for 1.5 hours, combine the filtrate, and concentrate to contain 1 g / mL of crude drug.

[0095] Comparative Example 3

[0096] Take 300 parts of Caulis Sinomenii and 300 parts of Millettia Spatholobi in weight, add 10 times the amount of water, decoct twice, each time for 1.5 hours, combine the filtrate, and concentrate to contain 1 g / mL of crude drug.

[0097] Test Example 1

[0098] The SPF chickens were divided into 14 groups, each with 20 SPF chickens. The 14 groups were: blank control group, model group, positive drug group, and drug groups 1 to 10 (used to administer 0.5 mL of the drug solution of Examples 1 to 8 and Comparative Examples 1, 2, and 3, respectively). The chickens in the positive drug group, model group, and drug groups 1 to 10 were challenged with synoviae (SCL strain of Mycoplasma synoviae, with a bacterial solution concentration of 1×10 9 CCU / mL), 5 days after the attack, the drug groups 1 to 10 were respectively gavaged with 0.5 mL of the drug solution of the above-mentioned Examples 1 to 8, Comparative Examples 1, 2, and 3, and the drug was administered continuously for 7 days. The lesions were observed on the second day of the last administration to determine the lesion scores of each group. The positive drug group took tylosin tartrate (administered according to the product instructions, purchased from Edison (Beijing) Biotechnology Co., Ltd.), and the blank group and the model group were gavaged with the same dose of water. The results were statistically analyzed according to the lesion scoring method of Test Example 1, and the results are shown in Table 3.

[0099] Table 3 Statistical results of lesion scores in each group

[0100]

[0101]

[0102] Note: Different letters indicate significant differences among the groups (P<0.05), and the same letters indicate no significant differences.

[0103] As shown in Table 3, the lesion scores of Examples 6 to 8 are close to those of the positive drug group, and can achieve the therapeutic effect of antibiotics. Compared with antibiotic treatment, the Chinese medicine composition of the present invention can also enhance the resistance of chickens and reduce the occurrence of recurrence.

[0104] Test case 2: The effect of clinical prevention and treatment of chicken synoviae mycoplasma in a large region

[0105] The incidence of Mycoplasma synoviae was determined by clinical symptoms of chickens and laboratory PCR detection, and the experimental site was selected. The experimental site was selected in a breeding group in Liaoning Province, with about 50,000 99-day-old young chickens, a total of 5 chicken houses, each with about 10,000 chickens.

[0106] Among them, sick chickens appeared in the flocks of Buildings 7 and 10 one after another, with an increase in the proportion of joint swelling and chest cysts, slow growth in feed intake, and stunted growth and development of chickens. PCR testing of the diseased samples found that the pathogen detection of the samples was positive for MS (synoviae Mycoplasma) (see Table 4, Figure 2 ).

[0107] Table 4. Pathogen detection results

[0108]

[0109] Note: “+” means positive; “-” means negative; “±” means doubtful.

[0110] The results showed that the chicken flocks in Buildings 7 and 10 were determined to be infected with Mycoplasma synoviae.

[0111] Medication test :

[0112] A chicken farm diagnosed with Mycoplasma synoviae infection was selected to carry out the effect verification of the present invention, and group comparison was used to test the clinical effect of the present invention.

[0113] The medication scheme is as follows: Building 7 is the experimental group, taking the liquid medicine of Example 7, 1L of the liquid medicine is diluted with 750L of water, and it is used up in 4 hours according to the daily drinking water; Building 10 is the control group, which is not given any medicine (in the past, the conventional treatment drugs (e.g., tylosin tartrate) used in farms with chicken synoviae mycoplasma disease were not ideal, so the control group did not use conventional drugs this time), and was fed daily.

[0114] The mental state of the chickens was observed by observing their heads after being frightened, and the state of the chickens before taking the medicine. Figure 4 As shown, the results showed that the mental state of the chickens improved after medication.

[0115] The specific clinical trial example verification results can be seen in Table 5 below.

[0116] Table 5: Production data and culled chickens

[0117]

[0118] As can be seen from Table 5, after the occurrence of synoviae Mycoplasma disease in the 7th chicken group, Example 7 was given drinking water for medication, and the mental state of the medicated chickens improved, the feed intake was significantly increased compared with the control group 10, and the number of chickens eliminated every day gradually decreased, while the number of chickens eliminated in the 10th chicken house of the control group did not show a significant decrease, and there was an increasing trend. The experiment proved that Example 7 can reduce the incidence of synoviae Mycoplasma in the chicken group, reduce the number of eliminated chickens, and solve the problem of difficult control when synoviae Mycoplasma disease occurs in the chicken group during breeding.

[0119] This application selects relatively low-priced preventive and therapeutic medicinal materials through dialectical analysis combined with the actual cost usage of veterinary drugs in clinical practice. The obtained formula is pure Chinese herbal ingredients with little toxic and side effects, no drug residue problems, no drug resistance problems, and a low price for clinical use.

[0120] Preferably, the weight proportions of the components of the pharmaceutical composition for preventing and treating chicken synoviae mycoplasma disease are: 300 parts of Caulis Sinomenii, 300 parts of Millettia reticulata and 40 parts of Cyathula capitata.

[0121] Clinical verification shows that the formula composition of the present invention has obvious effects of preventing and treating chicken synoviae mycoplasma disease, and it is verified that the optimal formula ratio of the present invention has obvious clinical effects.

[0122] The prescription of the invention has low cost and has the synergistic advantage of the prescription, has obvious effect in preventing and treating chicken synoviae disease, has the advantages of simplified prescription ingredients and convenient preparation, improves the cost performance of preventing and treating chicken synoviae disease, and improves economic benefits.

[0123] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A Chinese medicine composition for preventing and treating chicken synoviae mycoplasma disease, characterized in that: The active ingredients are composed of the following composition in parts by weight: 200-600 parts of Sinomenium spatholobi, 200-600 parts of Millettia spatholobi and 20-600 parts of Cyathula officinalis.

2. The Chinese medicine composition according to claim 1, characterized in that: The active ingredients are composed of the following composition in parts by weight: 200-600 parts of Sinomenium spatholobi, 200-600 parts of Millettia spatholobi and 20-60 parts of Cyathula officinalis.

3. The Chinese medicine composition according to claim 1, characterized in that The active ingredients are composed of the following composition in parts by weight: 200-400 parts of Sinomenium spatholobi, 200-400 parts of Millettia repens and 30-50 parts of Cyathula officinalis.

4. The Chinese medicine composition according to claim 1, characterized in that: The active ingredients are composed of the following composition in parts by weight: 300 parts of Sinomenium spatholobi, 300 parts of Millettia spatholobi and 30-50 parts of Cyathula capitata.

5. The Chinese medicine composition according to claim 1, characterized in that: The active ingredients are composed of the following composition in parts by weight: 300 parts of Sinomenium spatholobi, 300 parts of Millettia spatholobi and 40 parts of Cyathula capitata.

6. The Chinese medicine composition according to claim 1, characterized in that: The active ingredients are composed of the following composition in parts by weight: 300 parts of Sinomenium spatholobi, 300 parts of Millettia spatholobi and 30 parts of Cyathula capitata.

7. The Chinese medicine composition according to claim 1, characterized in that: The active ingredients are composed of the following composition in parts by weight: 300 parts of Sinomenium spatholobi, 300 parts of Millettia spatholobi and 50 parts of Cyathula capitata.

8. The Chinese medicine composition according to any one of claims 1 to 7, characterized in that: The dosage form of the Chinese medicine composition is any one of oral liquid, powder, spray, paste and capsule.

9. The Chinese medicine composition according to claim 8, characterized in that: The dosage form of the Chinese medicine composition is oral liquid.

10. The Chinese medicine composition according to claim 8, characterized in that: The amount of crude drug in the oral solution is 0.5-5 g / mL.

11. The Chinese medicine composition according to claim 8, characterized in that: The amount of crude drug in the oral solution is 0.8-2 g / mL.

12. The Chinese medicine composition according to claim 8, characterized in that: The amount of crude drug in the oral solution is 1-2 g / mL.

13. An oral liquid, characterized in that: Prepared according to any one of claims 1 to 12.

14. A method for preparing the Chinese medicine composition according to any one of claims 1 to 12, characterized in that: include: Caulis Sinomenii, Millettia Spatholobi and Cyathula capitata are mixed in proportion, and hot extraction and water extraction are performed to obtain a concentrated solution.

15. The preparation method according to claim 14, characterized in that: The hot extraction and water extraction method is: the extraction temperature is 70~100℃.

16. The preparation method according to claim 14 or 15, characterized in that: Add 5 to 15 times the amount of water, boil 1 to 3 times, and combine.

17. A chicken feed, characterized in that: The invention comprises a basic chicken feed and the Chinese medicine composition according to any one of claims 1 to 12, the oral liquid according to claim 13 or a product prepared by the preparation method according to any one of claims 14 to 16.

18. Use of the traditional Chinese medicine composition according to any one of claims 1 to 12, the oral liquid according to claim 13, the product prepared by the preparation method according to any one of claims 14 to 16, or the chicken feed according to claim 17 in the preparation of veterinary medicine products for preventing and treating chicken synoviae mycoplasma disease.

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