Synergistic insecticidal composition and method for preventing or controlling harmful organisms

Abstract

The present application relates to a synergistic insecticidal composition, which contains a synergistically effective amount of a combination of an active compound (A) isoxathiapyramide (Isocycloseram) and an active compound (B), wherein the active compound (B) is selected from at least one of spinosad and spinetoram. When the application rates of the active compound (A) and the active compound (B) are reduced, even when the active compound (A) and the active compound (B) become completely ineffective at a low application rate range, the insecticidal composition of the present application still exhibits a highly effective pest control effect, and an unexpected synergistic effect is obtained.

Description

Technical Field

[0001] The present invention relates to a synergistic insecticidal composition which is highly effective in controlling harmful organisms such as insects, mites, nematodes and mollusks. Background Art

[0002] The active ingredient of isocycloseram is known from WO2011067272A1. Its structural formula is represented by formula I. Isocycloseram can be used as a nematicide, acaricide, insecticide, acaricide, and / or molluscicide, for example.

[0003]

[0004] Although alternate application of pesticides with different modes of action can be adopted in good pest management practice, this method may not always achieve satisfactory pest control effects. In addition, the insecticide combinations that have been studied for pest control show that it is not easy to obtain high synergistic control effects.

[0005] In order to reduce or avoid adverse environmental or toxicological effects caused by the application of pesticides, the present application is intended to provide an insecticidal composition comprising isoxathiapiprole, which can provide effective pest control effects at a reduced dosage rate of the active ingredient. Summary of the invention

[0006] The present application provides a synergistic insecticidal composition, which contains a synergistically effective amount of a combination of an active compound (A) isoxathiapyr (Isocycloseram) and an active compound (B), wherein the active compound (B) is selected from at least one of spinosad and spinetoram.

[0007] Surprisingly, when the application rates of active compound (A) and active compound (B) are reduced, even when active compound (A) and active compound (B) become completely ineffective at a low application rate range, the synergistic insecticidal composition of the present application still exhibits a highly effective pest control effect, achieving an unexpected synergistic effect.

[0008] As used herein, the term "synergistic effect" refers to and includes the synergistic effect encountered when two or more active compounds are combined, wherein the combined activity of the two or more active compounds exceeds the sum of the activities of each active compound alone.

[0009] The presence of a synergistic effect was determined using the method described in Colby SR, "Calculating Synergistic and Antagonistic Responses of Herbicide Combinations," Weeds, 1967, 15, 20-22.

[0010] A synergistic effect exists when the effect of the combination of active ingredients is greater than the sum of the effects of the individual components.

[0011] For a given active compound combination, the effect to be expected using p+q ppm of active compound (A)+(B) is:

[0012] E = X + YX·Y / 100;

[0013] X = the percentage effect (%) of active ingredient (A) using p ppm of active ingredient;

[0014] Y = the percentage effect (%) of the active ingredient (B) using q ppm of active ingredient;

[0015] ppm = milligrams of active ingredient per litre of spray mixture (= ai).

[0016] If the actual observed effect (O) is greater than the expected effect (E), then the effect of the combination is superadditive.

[0017] As used herein, the term "synergistically effective amount" means and includes an amount of two or more active compounds that provides a synergistic effect as defined above.

[0018] In one embodiment, the synergistic insecticidal composition comprises a combination of an active compound (A) isocycloseram and an active compound (B), wherein the active compound (B) is selected from spinosad.

[0019] In one embodiment, the synergistic insecticidal composition comprises a combination of an active compound (A) isocycloseram and an active compound (B), wherein the active compound (B) is selected from spinetoram.

[0020] The weight ratio of the active compound (A) to the active compound (B) is generally in the range of 50:1-1:50, more preferably 25:1-1:25, more preferably 20:1-1:20, more preferably 10:1-1:10, more preferably 5:1-1:5, more preferably 4:1-1:4, more preferably 3:1-1:3, more preferably 2:1-1:2, more preferably 1:1 to obtain a synergistic effect.

[0021] The weight ratio of the active compound (A) to the active compound (B) described in the present application can also be, for example, 50:1, 45:1, 40:1, 35:1, 30:1, 25:1, 20:1, 19:1, 18:1, 17:1, 16:1, 15:1, 14:1, 13:1, 12:1, 11:1, 10:1, 9:1, 8:1, 7:1, 6:1, 5:1 :1, 4:1, 3:1, 2:1, 1:1, 1:2, 1:3, 1:4, 1:5, 1:6, 1:7, 1:8, 1:9, 1:10, 1:11, 1:12, 1:13, 1:14, 1:15, 1:16, 1:17, 1:18, 1:19, 1:20, 1:25, 1:30, 1:35, 1:40, 1:45, 1:50.

[0022] A synergistically effective amount of the combination of active compounds can be determined based on prevailing conditions including pest species, crop species, application mode, application timing, weather conditions, soil conditions, or terrain features.

[0023] The present application provides a use of the synergistic insecticidal composition for preventing or controlling insect, acarid, nematode or mollusk pests.

[0024] The synergistic insecticidal composition of the present application can be used to control at least one or more of the following harmful organisms: Class Insecta, Class Arachnida, Phylum Nematoda.

[0025] In some embodiments, the synergistic insecticidal composition of the present application can be used to control at least one or more of the following pests: Lepidoptera, Coleoptera, Hemiptera, Thysanoptera, Isoptera, Orthoptera, Diptera, Hymenoptera and Acari.

[0026] Pests of the order Thysanoptera, including, but not limited to, Thrips spp., e.g., onion thrips, Thrips hawaiiensis, Hawaiian flower thrips, western flower thrips, yellow tea thrips, tobacco thrips, Calio thrips phaseoli, greenhouse thrips, Riphiphoro thrips cruentatus, citrus thrips.

[0027] The order of Hemiptera includes, but is not limited to, grapephylloxera, Sitobio navenae, cornleaf aphid, Rhapalosiphumpadi, potato aphid, Macrosiphumrosae, rose grain aphid, Myzus persicae, Brevicorynebrassicae, Acrythosiphon pisum, woolly apple aphid, Aphis gossypii, apple aphid, citricola scale, Icerya purchasi, Nephotettix cinctipes, mango leaf hopper hopper), Laodelphax striatellus, brown planthopper, Paratrioza cockerelli, tomato psyllid, spruce budscale, citrus mealybug, grape mealybug, Pseudococcus spp. (mealybug), Pseudococcus brevipes (pineapple mealybug), bug), pear orchard shield scale (San Jose scale), white-backed planthopper, greenhouse whitefly, banded wing whitefly (Trialeurodes abutiloneus), cabbage whitefly (Aleurodes proletella), spiral whitefly (Aleurodicus disperses), velvet whitefly (Aleurothrixus floccosus), cotton whitefly (woolly whitefly), silverleaf whitefly (Bemisia argentifolii), sweet potato whitefly (Bemisia tabaci).

[0028] Lepidoptera pests: including but not limited to Pyralidae, such as Chilo suppressalis (walker), Tryporyza incertulas (walker), Cnaphalocrocismedinalis Guenee, Hellullaundalis, Conogethes punctiferlis; Pieridae, such as Pieris rapae, rapae, papilioxuthus, pierisrapae crucivora, parnaraguttata; Arctiidae, such as the white moth (hyphantriacunea); Noctuidae, such as the cabbage looper (trichoplusiani), cabbage looper (mamestrabrassicae), beet armyworm (spodoptera exigua), cutworm (spodopteralitura), cotton bollworm (helicoverpa armigera), oriental armyworm (Pseudaletiaseparate), cutworm (Agrotis ipsilon); Tortricidae, such as the tea leaf roller (adoxophyes orana fasciata), archips fuscocureanus, homona magnanima; Plutellidae, for example, Plutellax sylotella; Gelechiidae, for example, Pectinophora gossypiella, Pink bollworm;

[0029] Acari: includes, but is not limited to, Olignychus coffee, Olignychus silicus, southern red mite, Panonychus citri, citrus red mite, Panonychus ulmi, Phyllocoptruta oleivora, citrus rust mite, two spotted spidermite, Acarus siro, grain mite, mango bud mite, tomato russet mite, Aculus pelekassi, apple rustmite, privet mite, Eotetranychus carpini, yellows pider mite), Epitimerus spp., Eriophyes spp., Ixodes spp. (ticks).

[0030] Nematoda pests: including, but not limited to, Ditylenchus spp. (stem bulb nematodes), Heterodera spp. (cyst nematodes), Heteroderazeae (corn cyst nematodes), Hirschmanniella spp. (root nematodes), Hoplolaimus spp. (lance nematodes), Meloidogyne spp. (root knot nematodes), notnematodes), Meloidogyne incognita (root-knot nematodes), Aphelenchoides spp. (leaf nematodes), Belonolaimus spp. (sting nematodes), Criconemella spp. (ring nematodes), Onchocerca volvulus (hook-tailworm), Pratylenchus spp. (lesion nematodes), burrowing nematodes.

[0031] In a further embodiment, the synergistic insecticidal composition described herein is applied near an insect population, wherein the pests include at least one of the following: diamondback moth, aphids, and thrips.

[0032] The present application provides a method for preventing or controlling pests, comprising applying the synergistic insecticidal composition described in the present application to the pests, the locations of the pests, or plants susceptible to attack by the pests.

[0033] A method for preventing or controlling harmful organisms comprises applying the synergistic insecticidal composition described in the present application to leaves, fruit parts, and soil of plants.

[0034] A method for preventing or controlling pests, comprising directly contacting the synergistic pesticidal composition described in the present application with the pests.

[0035] The area to which the synergistic insecticidal composition of the present application is applied may be any area infested by pests, for example, areas where crops, trees, fruits, cereals, fodder, vines, grasses and ornamental plants grow.

[0036] Specific crop areas to which the synergistic insecticidal composition of the present application is applied include areas where apples, corn, sunflowers, cotton, soybeans, canola, wheat, rice, sorghum, barley, oats, potatoes, oranges, alfalfa, lettuce, strawberries, tomatoes, peppers, crucifers, pears, tobacco, almonds, beets, beans and other valuable crops are grown or their seeds are about to be sown.

[0037] The present application provides a method for preventing or controlling pests, comprising applying the synergistic pesticidal composition of the present application in an insecticidal effective amount to the pests, the locations of the pests, or to plants susceptible to attack by the pests.

[0038] The synergistic insecticidal composition of the present application can be applied to the leaves and fruit parts of plants to control pests, and the insecticidal composition will directly contact the pests.

[0039] The synergistic insecticidal composition of the present application can also be applied to the soil and when applied in this manner, can control pests that attack roots and stems. The roots can absorb the active compound molecules and absorb them into the leaf parts of the plant to control above-ground chewing mouthparts pests and sap-feeding pests.

[0040] The synergistic insecticidal composition of the present application can also be applied to different parts of the plant, and pests can be controlled in another part of the plant by means of the systemic movement of the active compound molecules in the plant. For example, control of leaf-feeding insects can be achieved by treating seeds before planting, by drip irrigation application or furrow application, by watering the soil with, for example, soil before or after planting.

[0041] The synergistic insecticidal composition of the present application is generally used for control at an application rate of 1 g ai / ha to about 500 g ai / ha, preferably at an application rate of 5 g ai / ha to about 250 g ai / ha, and more preferably at an application rate of 10 g ai / ha to about 150 g ai / ha.

[0042] The application rate of the synergistic insecticidal composition of the present application will depend on different factors, such as the compound used; the object of treatment, such as, for example, plants, soil or seeds; the type of treatment, such as, for example, spraying, dusting or seed dressing; the purpose of treatment, such as, for example, preventive or curative; the type of pest to be controlled or the time of application.

[0043] In order to facilitate application, handling, transportation, storage and maximize the activity of the pesticide, other auxiliary ingredients are usually added to the active ingredient so that the pesticide can be used at the required concentration and in an appropriate form.

[0044] The formulation type selected in any embodiment will depend on the specific purpose under consideration and the physical, chemical and biological properties of the active ingredients. The pesticidal composition of the present application can be selected from a variety of formulation forms, including powderable powders (DP), soluble powders (SP), water-soluble granules (SG), water-dispersible granules (WG), wettable powders (WP), granules (GR) (slow release or fast release), soluble concentrates (SL), oil-soluble liquids (OL), ultra-low volume liquids (UL), emulsifiable concentrates (EC), dispersible concentrates (DC), emulsions (both oil-in-water (EW) and water-in-oil (EO)), microemulsions (ME), suspension concentrates (SC), sprays, atomizer / smoke formulations, capsule suspensions (CS) and seed treatment formulations.

[0045] The synergistic insecticidal composition of the present application may optionally contain auxiliary ingredients such as agronomically acceptable surfactants and / or carriers or diluents. Preferably, the auxiliary ingredients are one or a mixture of emulsifiers, dispersants, wetting agents, penetrants, antifreeze agents, anti-drift agents, thickeners, defoamers, disintegrants, binders, and auxiliary carriers. Generally speaking, these formulations include from 0.01% to 90% of active ingredients, from 0 to 20% of agriculturally acceptable surfactants, and 10% to 99.99% of carriers or diluents and one (or more) adjuvants by weight.

[0046] Surfactants are sometimes used alone or with other additives (such as mineral or vegetable oils as adjuvants in spray tank mixes) to improve the biological performance of pesticides against target species. The type of surfactant used for bioenhancement generally depends on the nature and mode of action of the pesticide.

[0047] Examples of surfactants that can be used include fatty alcohol polyoxyethylene ethers, polyoxyethylene alkyl aryl ethers, polyoxyethylene higher fatty acid esters, phosphoric acid esters of polyoxyethylene alcohols or phenols, fatty acid esters of polyols, naphthalenesulfonic acid polymers, lignin sulfonates, high molecular weight comb-shaped branched copolymers, butylnaphthalenesulfonates, alkylarylsulfonates, alkylsulfosuccinates, oils and fats, condensates of fatty alcohols and ethylene oxide, polyacrylates of alkylnaphthalenesulfonates, protein hydrolysates, suitable oligosaccharides or polymers, for example based on single vinyl monomers, acrylic acid, polyoxyethylene and / or polyoxypropylene or combinations thereof with, for example, (poly)alcohols or (poly)amines.

[0048] The carrier or diluent in an agricultural formulation is a substance added to the pesticide to obtain a product of the desired strength. A carrier is generally a substance with a high absorption capacity, while a diluent is generally a substance with a low absorption capacity. Therefore, the carrier or diluent is preferably inert and at least agronomically acceptable. The carrier or diluent may be solid or liquid.

[0049] As the solid carrier or diluent, there may be used, for example, talc, bentonite, clay, kaolin, diatomaceous earth, white carbon, calcium carbonate, acid clay, silica, zeolite, attapulgite, pumice, ammonium sulfate, sodium sulfate and urea.

[0050] Examples of liquid carriers or diluents that can be used include water, isopropanol, butanol, tetrahydrofuran, ethylene glycol, propylene glycol, ethyl acetate, methyl oleate, dimethylformamide, dimethylacetamide, dimethyl sulfoxide, and vegetable oils.

[0051] The synergistic insecticidal composition described herein exhibits a synergistic effect, and a lower insecticidally effective amount of the composition provides a superior pest control effect when compared to using the active compound (A) or the active compound (B) alone.

[0052] The synergistic pesticidal composition described herein can have high synergistic pest control and allow for lower effective dosage rates, increased environmental safety, and reduced incidence of pest resistance.

[0053] In addition, the synergistic insecticidal composition of the present application can effectively combat pests without substantially causing any harmful side effects to the planted plants. The application of the composition of the present application can increase the harvest yield and improve the quality of the harvested crops.

[0054] Compared with the prior art, this application has at least the following beneficial effects:

[0055] The synergistic insecticidal composition provided in the present application still exhibits a highly effective pest control effect when the application rates of active compound (A) and active compound (B) are reduced, and even when active compound (A) and active compound (B) become completely ineffective at a low application rate range, thereby achieving an unexpected synergistic effect. DETAILED DESCRIPTION

[0056] To facilitate understanding of the present application, the present application lists the following embodiments. Those skilled in the art should understand that the embodiments are only to help understand the present application and should not be regarded as specific limitations of the present application.

[0057] Biological test cases

[0058] Test 1: Thrips test

[0059] Preparation of drug solution: prepare the original drugs of isoxathiapiprolin, spinosad and ethyl spinetoram into mother solution with acetone, and then dilute it with 0.1% Tween-80 aqueous solution to the required concentration.

[0060] Test object: Flower thrips. The test site is located in the cowpea planting area of ​​Yangmai Village, Dingcheng Town, Ding'an County, Hainan Province. The village grows cowpeas every winter, and over the years, thrips have caused serious damage. The planting site is red soil, the soil is fertile, the terrain is relatively flat, and the drainage and irrigation conditions are good. The planting method of the test site is ridge planting, the ridge is covered with plastic film, and double rows and double plants are planted. When the pesticide was applied, the cowpea growth period in the test site was the early fruiting period, and the cowpea plants grew normally. The area of ​​the plot is 36m 2 . Repeat 3 times.

[0061] The whole plant was sprayed with a backpack WS-12D electric sprayer, a conical nozzle, a nozzle diameter of 1.5mm, and a flow rate of 0.85L / min. The electric sprayer was used for uniform spraying, and the spray volume was such that the leaves and fruits were slightly dripping. The test used a diagonal five-point sampling method to investigate, with 5 points investigated in each plot, 2 plants marked at each point, and the upper, middle, and lower flowers of each cowpea plant investigated respectively. Ten cowpea plants were fixedly investigated in each plot, and the number of thrips was counted. The base number of thrips in the field was investigated before application, and the control effect was investigated 7 days after the application. A blank control of clean water was used.

[0062] The efficacy calculation method is as follows:

[0063]

[0064]

[0065] Table 1 Control effect on flower thrips

[0066]

[0067] Test 2 Diamondback moth test

[0068] Preparation of drug solution: prepare isoxathiapiprolin, spinosad and spinetoram with acetone to prepare mother solution, and then dilute with 0.1% Tween-80 aqueous solution to the required concentration.

[0069] Choice of crops and cultivars: Cruciferous vegetable kale (variety Qiuqi).

[0070] Selection of experimental pests: Plutella xylostella.

[0071] The test site was arranged at the Vegetable Research Institute of Zhongluotan Academy of Agricultural Sciences in Guangdong Province. The test plot was sandy loam with the same water and fertilizer management level.

[0072] Plot setting: The experiment has 9 treatments and 3 replicates. The plot area is about 20m 2 , arranged in randomized blocks.

[0073] Application equipment: Linong HD400 backpack manual sprayer.

[0074] When applying the pesticide, spray evenly on the upper and lower surfaces of the leaves of the whole plant. Investigate the insect population before applying the pesticide. Investigate the number of live insects 3 days after applying the pesticide.

[0075] Calculation method of drug efficacy:

[0076]

[0077]

[0078] Table 2 Control effect on diamondback moth

[0079]

[0080] Test 3: Aphid test

[0081] Preparation of drug solution: prepare isoxathiapiprolin, spinosad and spinetoram with acetone to prepare mother solution, and then dilute with 0.1% Tween-80 aqueous solution to the required concentration.

[0082] Crop and cultivar selection: Cabbage, Jingfeng No. 1 BBCH 15-17.

[0083] Selection of test pests: wingless aphids of natural populations in the field of radish aphids (Lipaphis erysimi).

[0084] Cultivation conditions: The test site was arranged in the experimental field of Kunshan Dongfang Village Farm, and the previous crop was wasteland. The planting density per mu was 2,500 plants / mu, and other fertilization and irrigation were consistent with local conditions.

[0085] Area: 4.32m 2 / cell; number of repetitions: 3 times, with random block arrangement.

[0086] Application equipment: Spray: CO2 sprayer, application pressure 3BAR, water consumption 450L / ha.

[0087] Application method: Spray evenly on the upper and lower surfaces of leaves of the whole plant.

[0088] Aphid counts were conducted on all leaves of the plant before and 7 days after application of the pesticide, with 8 plants surveyed in each plot. Plants with fixed insects were selected before the test and marked with small yellow clips.

[0089] Calculation method of drug efficacy:

[0090]

[0091]

[0092] Table 3 Control effect on radish aphid

[0093]

[0094] The test results in Tables 1 to 3 show that the combination of isoxathiapiprolin and spinosad or spinetoram has an unexpected synergistic effect on the control of thrips, diamondback moth and aphids.

Claims

1. A synergistic insecticidal composition, wherein the active components are composed of the active compound (A) isoxathiapiprole and the active compound (B) as shown in formula I; The active compound (B) is at least one selected from spinosad and ethyl spinosad; The weight ratio of the active compound (A) to the active compound (B) is 10:1-1:

10.

2. The synergistic insecticidal composition according to claim 1, wherein: The weight ratio of the active compound (A) to the active compound (B) is 5:1-1:

5.

3. The synergistic insecticidal composition according to claim 1, wherein: The weight ratio of the active compound (A) to the active compound (B) is 2:1-1:

2.

4. The synergistic insecticidal composition according to claim 1, wherein: The pesticidal composition further comprises at least one of a surfactant, a carrier or a diluent.

5. Use of the synergistic insecticidal composition according to claim 1 for preventing or controlling diamondback moth, thrips or aphids.

6. A method for preventing or controlling pests, comprising applying the synergistic insecticidal composition of claim 1 to the pests, the locations of the pests, or plants susceptible to attack by the pests; The harmful organisms are diamondback moth, thrips or aphids.

7. A method for preventing or controlling harmful organisms, comprising applying the synergistic insecticidal composition of claim 1 to leaves, fruit parts or soil of plants; The harmful organisms are diamondback moth, thrips or aphids.

8. A method for preventing or controlling harmful organisms, comprising directly contacting the synergistic insecticidal composition of claim 1 with harmful organisms; the harmful organisms are diamondback moth, thrips or aphids.

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