A prebiotic composition for regulating skin flora and its preparation method and use

Abstract

The invention discloses a prebiotic composition for regulating skin flora and its preparation method and use, relates to the field of biomedical technology, including inulin, α-glucan oligosaccharide, white willow bark extract, honeysuckle extract; the preparation method is: after white willow bark extract and honeysuckle extract are mixed, inulin is added to stir and mix, and finally α-glucan oligosaccharide is added to mix to obtain a prebiotic composition; it can be used for the preparation of male and female private parts washing liquid. The formula of the prebiotic composition is used in external products, can play an effective antibacterial effect, inhibit Staphylococcus aureus, Candida albicans, Escherichia coli, competitively provide energy for private parts beneficial bacteria, protect private parts beneficial lactobacillus, and can produce the effect of reducing sensitivity and improving private parts discomfort to male and female private parts respectively.

Description

Technical Field

[0001] The present invention relates to the field of biomedical technology, and in particular to a prebiotic composition for regulating skin flora, and a preparation method and use thereof. Background Art

[0002] Human skin is a large heterogeneous organ. Under normal physiological conditions, it can protect the body from the invasion of pathogenic microorganisms and maintain the balance of microbial flora that affect human health and disease [Elizabeth AG et al, Science, 2009, 324 (5931): 1190-1192]. Prebiotics can have a beneficial effect on the host by selectively stimulating the growth and activity of probiotic bacteria, thereby improving the health of the host. Studies have shown that prebiotics can help maintain the natural protective barrier of the skin surface, increase the skin's resistance to harmful bacteria, maintain the skin's natural balance, and reduce the occurrence of skin problems.

[0003] The vaginal flora is dominated by Lactobacillus. For a long time, the presence of Lactobacillus has been considered a necessary condition for the healthy vaginal microbial flora of women of childbearing age. A decrease or lack of lactobacilli in the vagina is associated with a high risk of endometritis and pelvic inflammatory disease. Various types of female vaginitis have varying degrees of vaginal flora imbalance.

[0004] The male private reproductive tract may also have bacterial flora problems. The National Institutes of Health (NIH) of the United States announced that the presence of microbial flora in the upper urogenital tract is an important cause of prostatitis. The main pathogens of acute bacterial prostatitis and chronic bacterial prostatitis are Escherichia coli and Staphylococcus.

[0005] There are currently many raw materials for regulating skin flora, but when added to topical products, they cannot actually achieve the effect of regulating flora and alleviating discomfort in the private parts. Summary of the invention

[0006] The technical problem to be solved by the present invention is that the current raw materials for regulating skin flora cannot actually achieve the effect of regulating flora and alleviating discomfort in private parts when added to external products. The purpose is to provide a prebiotic composition for regulating skin flora and a preparation method and use thereof, which solves the problem that the current raw materials for regulating skin flora cannot actually achieve the effect of regulating flora and alleviating discomfort in private parts when added to external products.

[0007] The present invention is achieved through the following technical solutions:

[0008] In a first aspect, the present application provides a prebiotic composition for regulating skin flora, comprising inulin, α-glucan oligosaccharide, white willow bark extract, and honeysuckle extract.

[0009] The formula of the prebiotic composition is used in external products, and can exert an effective antibacterial effect, inhibit Staphylococcus aureus, Candida albicans, and Escherichia coli, while competitively providing energy for beneficial bacteria in the private parts, protecting beneficial lactobacilli in the private parts, and having the effects of reducing sensitivity and improving discomfort in the private parts of men and women, respectively.

[0010] Furthermore, in terms of mass percentage, the amount of inulin in the prebiotic composition is 50% to 70%, the amount of α-glucan oligosaccharide is 10% to 30%, the amount of white willow bark extract is 0% to 15%, and the amount of honeysuckle extract is 5% to 20%.

[0011] Furthermore, by mass percentage, the amount of inulin in the prebiotic composition is 60%, the amount of α-glucan oligosaccharide is 15%, the amount of white willow bark extract is 10%, and the amount of honeysuckle extract is 15%.

[0012] Furthermore, the extraction method of the white willow bark extract is:

[0013] Step 1: The dried bark of the white willow tree is selected, processed, crushed, and filtered to obtain a medicinal powder;

[0014] Step 2: Add alcohol to the drug powder, stir in a closed manner, extract at a constant temperature and filter to obtain a first filtrate and a first filter residue;

[0015] Step 3: Add alcohol to the first filter residue, stir in a closed manner, extract at a constant temperature and filter to obtain a second filtrate and a second filter residue;

[0016] Step 4: combining the first filtrate and the second filtrate, standing for more than 12 hours to obtain a supernatant, recovering the alcohol, and filtering the supernatant to obtain a crude extract of white willow bark;

[0017] Step 5: prepare the crude extract of white willow bark into acid solution, add chloroform, stir in a closed container and let stand, then extract and separate to obtain an upper filtrate and a lower chloroform solution;

[0018] Step 6: Add the upper filtrate into chloroform, stir it in a sealed container and let it stand, extract and separate to obtain the upper medicinal solution, and filter the upper medicinal solution to obtain the white willow bark extract.

[0019] Furthermore, the amount of alcohol added in step 2 is 3 times that of the medicinal powder, and the alcohol concentration is 70%; the amount of alcohol added in step 3 is 2.5 times that of the first filter residue, and the alcohol concentration is 70%; the constant temperature in steps 2 and 3 is 40°C; the pH value of the acid solution in step 5 is 4.0-5.0; the amount of chloroform added in step 5 is 3 times that of the crude liquid extracted from white willow bark; the amount of chloroform added in step 6 is 3 times that of the upper filtrate.

[0020] Furthermore, the extraction method of the honeysuckle extract is:

[0021] Step 1: Use the dried flowers of honeysuckle as raw materials, select, process, crush and filter them to obtain medicinal powder;

[0022] Step 2: Add alcohol to the drug powder, stir in a closed manner, extract at a constant temperature and filter to obtain a first filtrate and a first filter residue;

[0023] Step 3: Add alcohol to the first filter residue, stir in a closed manner, extract at a constant temperature and filter to obtain a second filtrate and a second filter residue;

[0024] Step 4: combining the first filtrate and the second filtrate, standing for more than 12 hours to obtain a supernatant, recovering the alcohol, and filtering the supernatant to obtain a crude honeysuckle extract;

[0025] Step 5: prepare the crude extract of honeysuckle into acid solution, add chloroform, stir in a closed container and let stand, then extract and separate to obtain an upper filtrate and a lower chloroform solution;

[0026] Step 6: Add the upper filtrate into chloroform, stir in a sealed container and let it stand to obtain an upper medicinal solution after extraction and separation. Filter the upper medicinal solution to obtain the honeysuckle extract.

[0027] Furthermore, the amount of alcohol added in step 2 is 3 times that of the medicinal powder, and the alcohol concentration is 65%; the amount of alcohol added in step 3 is 1.5 times that of the first filter residue, and the alcohol concentration is 65%; the constant temperature in steps 2 and 3 is 60°C; the pH value of the acid solution in step 5 is 5.0-6.0; the amount of chloroform added in step 5 is 3 times that of the crude liquid extracted from white willow bark; the amount of chloroform added in step 6 is 3 times that of the upper filtrate.

[0028] In a second aspect, the present application provides a method for preparing a prebiotic composition for regulating skin flora, comprising mixing white willow bark extract and honeysuckle extract, adding inulin and stirring, and finally adding α-glucan oligosaccharide and stirring to obtain the prebiotic composition.

[0029] In a third aspect, the present application proposes a prebiotic composition for regulating skin flora and a preparation method thereof, wherein the prebiotic composition for regulating skin flora is used to prepare female private parts wash.

[0030] In a fourth aspect, the present application proposes the above-mentioned prebiotic composition for regulating skin flora and the preparation method thereof. The prebiotic composition for regulating skin flora is used to prepare male private parts wash.

[0031] Compared with the prior art, the present invention has the following advantages and beneficial effects:

[0032] The formula of the prebiotic composition is used in external products, and can exert an effective antibacterial effect, inhibit Staphylococcus aureus, Candida albicans, and Escherichia coli, while competitively providing energy for beneficial bacteria in the private parts, protecting beneficial lactobacilli in the private parts, and having the effects of reducing sensitivity and improving discomfort in the private parts of men and women, respectively. DETAILED DESCRIPTION

[0033] Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of them.

[0034] Example 1

[0035] This embodiment provides a prebiotic composition for regulating skin flora, which contains, by mass percentage, 60% inulin, 15% α-glucan oligosaccharide, 10% white willow bark extract, and 15% honeysuckle flower extract.

[0036] Wherein, the preparation process of white willow bark extract is:

[0037] Step 1: The dried bark of the white willow tree is selected, processed, crushed, and filtered with a 50-mesh cloth to obtain a medicinal powder;

[0038] Step 2: Add 3 times 70% alcohol to the powder, stir in a sealed container for 0.5 hours, extract at 40°C for 2 hours and filter to obtain the first filtrate and the first filter residue;

[0039] Step 3: Add 2.5 times 70% alcohol to the first filter residue, stir in a sealed container, extract at 40°C for 2 hours and filter to obtain a second filtrate and a second filter residue;

[0040] Step 4: combining the first filtrate and the second filtrate, standing for more than 12 hours to obtain a supernatant, recovering the alcohol, and filtering the supernatant to obtain a crude extract of white willow bark;

[0041] Step 5: prepare the crude extract of white willow bark into 3 times acid solution with a pH value of 4.0, add chloroform, stir in a closed container for 30 minutes, let stand for 3 hours, and then extract and separate to obtain an upper filtrate and a lower chloroform solution;

[0042] Step 6: Add the upper filtrate into 3 times chloroform and stir for 30 minutes, then let it stand for 3 hours to obtain the upper drug solution after extraction and separation. Filter the upper drug solution at 0.22 μm to obtain the white willow bark extract.

[0043] Wherein, the preparation process of honeysuckle flower extract is:

[0044] Step 1: Use the dried flowers of honeysuckle as raw materials, select, process and crush them, and then filter them with a 50-mesh cloth to obtain medicinal powder;

[0045] Step 2: Add 3 times 65% alcohol to the powder, stir in a sealed container for 0.5 hour, extract at 60°C for 2 hours and filter to obtain the first filtrate and the first filter residue;

[0046] Step 3: Add 1.5 times 65% alcohol to the first filter residue, stir in a sealed container, extract at 60°C for 2 hours and filter to obtain a second filtrate and a second filter residue;

[0047] Step 4: combining the first filtrate and the second filtrate, standing for more than 12 hours to obtain a supernatant, recovering the alcohol, and filtering the supernatant to obtain a crude honeysuckle extract;

[0048] Step 5: The crude extract of honeysuckle flower is prepared into 3 times of acid solution with a pH value of 5.0, and 3 times of chloroform is added. After stirring for 30 minutes in a closed container, it is allowed to stand for 3 hours, and then extracted and separated to obtain an upper filtrate and a lower layer of chloroform solution;

[0049] Step 6: Add the upper filtrate into 3 times chloroform and stir for 30 minutes, then let it stand for 3 hours to obtain the upper drug solution after extraction and separation. Filter the upper drug solution at 0.22 μm to obtain the honeysuckle extract.

[0050] The preparation method of the prebiotic composition comprises the following steps: mixing the willow bark extract and the honeysuckle flower extract, adding inulin and stirring and mixing, and finally adding alpha-glucan oligosaccharide and mixing to obtain the prebiotic composition.

[0051] Example 2

[0052] Based on Example 1, this example provides a prebiotic composition for regulating skin flora, which is different from Example 1 in that the formula of this example contains 70% inulin, 10% α-glucan oligosaccharide, and 20% honeysuckle extract by mass percentage.

[0053] In the preparation process of the honeysuckle flower extract, the pH value of the acid solution in step 5 is 5.5. Other process conditions and methods are the same as those in Example 1.

[0054] Example 3

[0055] Based on Example 1, this example provides a prebiotic composition for regulating skin flora, which is different from Example 1 in that the formula of this example contains 50% inulin, 30% α-glucan oligosaccharide, 10% white willow bark extract, and 10% honeysuckle extract by mass percentage.

[0056] In the preparation process of the white willow bark extract, the pH value of the acid solution in step 5 is 4.5; in the preparation process of the honeysuckle flower extract, the pH value of the acid solution in step 5 is 6. Other process conditions and methods are the same as those in Example 1.

[0057] Example 4

[0058] Based on Example 1, this example provides a prebiotic composition for regulating skin flora, which is different from Example 1 in that the formula of this example contains 60% inulin, 10% α-glucan oligosaccharide, 10% white willow bark extract, and 20% honeysuckle extract by mass percentage.

[0059] In the preparation process of the white willow bark extract, the pH value of the acid solution in step 5 is 5.0; in the preparation process of the honeysuckle flower extract, the pH value of the acid solution in step 5 is 5.0. Other process conditions and methods are the same as those in Example 1.

[0060] Example 5

[0061] Based on Example 1, this example provides a prebiotic composition for regulating skin flora, which is different from Example 1 in that the formula of this example contains 60% inulin, 30% α-glucan oligosaccharide, 5% white willow bark extract, and 5% honeysuckle extract by mass percentage.

[0062] In the preparation process of the white willow bark extract, the pH value of the acid solution in step 5 is 5.0; in the preparation process of the honeysuckle flower extract, the pH value of the acid solution in step 5 is 6.0. Other process conditions and methods are the same as those in Example 1.

[0063] Experimental Example 1

[0064] 50 g of the prebiotic composition prepared in Example 1 was mixed and dissolved in the basic intimate wash formula, and 0.3% by mass of chlorhexidine acetate was added to prepare 1000 g of intimate wash.

[0065] The formula of the basic intimate wash is: by mass fraction, 3% glycerin, 2.4% sodium lauroyl sarcosinate, 5% decyl glucoside, 1.5% cocamidopropyl betaine, 1% propylene glycol, 0.4% Thor PHCH preservative, and the rest water.

[0066] Experimental Example 2

[0067] 50 g of the prebiotic composition prepared in Example 1 was mixed and dissolved in a basic intimate wash formula to prepare 1000 g of intimate wash.

[0068] The formula of the basic intimate wash is: by mass fraction, 3% glycerin, 2.4% sodium lauroyl sarcosinate, 5% decyl glucoside, 1.5% cocamidopropyl betaine, 1% propylene glycol, 0.4% Thor PHCH preservative, and the rest water.

[0069] Experimental Example 3

[0070] 50 g of the prebiotic composition prepared in Example 1 was dissolved in purified water to prepare 1000 g of private care wash.

[0071] Experimental Example 4

[0072] 50 g of the prebiotic composition prepared in Example 2 was dissolved in purified water to prepare 1000 g of private care wash.

[0073] Experimental Example 5

[0074] 50 g of the prebiotic composition prepared in Example 3 was mixed and dissolved in the basic private area wash formula to prepare 1000 g of private area wash.

[0075] The formula of the basic intimate wash is: by mass fraction, 3% glycerin, 2.4% sodium lauroyl sarcosinate, 5% decyl glucoside, 1.5% cocamidopropyl betaine, 1% propylene glycol, 0.4% Thor PHCH preservative, and the rest water.

[0076] Experimental Example 6

[0077] 50 g of the prebiotic composition prepared in Example 4 was mixed and dissolved in the basic private area wash formula to prepare 1000 g of private area wash.

[0078] The formula of the basic intimate wash is: by mass fraction, 3% glycerin, 2.4% sodium lauroyl sarcosinate, 5% decyl glucoside, 1.5% cocamidopropyl betaine, 1% propylene glycol, 0.4% Thor PHCH preservative, and the rest water.

[0079] Experimental Example 7

[0080] 50 g of the prebiotic composition prepared in Example 5 was mixed and dissolved in the basic private area wash formula to prepare 1000 g of private area wash.

[0081] The formula of the basic intimate wash is: by mass fraction, 3% glycerin, 2.4% sodium lauroyl sarcosinate, 5% decyl glucoside, 1.5% cocamidopropyl betaine, 1% propylene glycol, 0.4% Thor PHCH preservative, and the rest water.

[0082] Comparative Example 1

[0083] Basic intimate wash, the formula is: by mass fraction, 3% glycerin, 2.4% sodium lauroyl sarcosinate, 5% decyl glucoside, 1.5% cocamidopropyl betaine, 1% propylene glycol, 0.4% Thor PHCH preservative, and the rest is water.

[0084] Comparative Example 2

[0085] The antibacterial private wash was prepared by adding 0.3% by mass of chlorhexidine acetate into the basic private wash formula.

[0086] The basic intimate wash has the following formula: by mass fraction, 3% glycerin, 2.4% sodium lauroyl sarcosinate, 5% decyl glucoside, 1.5% cocamidopropyl betaine, 1% propylene glycol, 0.4% Thor PHCH preservative, and the rest water.

[0087] In vitro testing

[0088] 1. Materials

[0089] Sample: Private care wash prepared in Experimental Example 2.

[0090] Test strains: Escherichia coli (8099), take its 6th generation fresh slant culture for testing; Staphylococcus aureus (ATCC 6538), take its 6th generation fresh slant culture for testing; Candida albicans (ATCC10231), take its 6th generation fresh slant culture for testing. The above strains are provided by Guangdong Food Microbiological Safety Engineering Technology Research and Development Center.

[0091] Instruments and equipment: KC-SP-YQ-179 electric constant temperature incubator (DHP-9271), KC-SP-YQ-720 biological safety cabinet (BSC-1604II A2)

[0092] 2. Methods

[0093] 1) Inspection basis: Appendix C4 of GB15979-2002 Hygiene Standards for Disposable Sanitary Products.

[0094] 2) Test conditions: temperature 21-23°C, relative humidity 52-55%.

[0095] 3) Sample preparation: as is.

[0096] 3. Results

[0097] The inhibition rate data of the original sample against Escherichia coli (8099), Staphylococcus aureus (ATCC 6538), and Candida albicans (ATCC10231) at 2 minutes, 5 minutes, 10 minutes, and 20 minutes are shown in the following Tables 1 to 3, respectively.

[0098] Table 1 Antibacterial effect of original samples on Escherichia coli (8099)

[0099]

[0100] Note: Negative control showed no sterile growth.

[0101] Table 2 Antibacterial effect of the original samples on Staphylococcus aureus (ATCC 6538)

[0102]

[0103] Note: Negative control showed no sterile growth.

[0104] Table 3 Antibacterial effect of original samples on Candida albicans (ATCC 10231)

[0105]

[0106] Note: Negative control showed no sterile growth.

[0107] Conclusion: According to GB 15979-2002 "Hygiene Standard for Disposable Sanitary Products", under experimental conditions, the average inhibition rate of the original sample for 2min, 5min, 10min, and 20min against Escherichia coli was greater than 99.98%, and all had a strong antibacterial effect; the average inhibition rate against Staphylococcus aureus was greater than 99.97%, and all had a strong antibacterial effect; the average inhibition rate against Candida albicans was greater than 99.97%, and all had a strong antibacterial effect.

[0108] The private care washes prepared in Experimental Examples 3 to 7 were subjected to competitive growth inhibition experiments with Lactobacillus rhamnosus and Candida albicans, wherein the private care wash prepared in Example 3 was subjected to two repeated experiments, namely Group A and Group B.

[0109] Bacterial count: After the bacteria are cultured to the logarithmic growth phase, 100 μL is taken and diluted with sterile water to 10 -5 , 10 -6 , 10 -7 Dilution gradient was spotted onto PDA agar plate (Candida albicans), and 10 -2 , 10 -3 , 10 -4 The dilution gradient was dripped onto TSA + 5% sterile defibrinated sheep blood agar plate (Gardnerella vaginalis), with 100 μL added each time. Two parallel controls were performed for each dilution. After 24 to 48 hours, the viable bacterial concentration (CFU / mL) of each group of bacteria was calculated and converted into the logarithm of the colony count (logCFU).

[0110] Table 4

[0111]

[0112]

[0113] The private care wash in Experimental Example 3 was used to conduct two repeated experiments on vaginal Gardnerella, namely Group A and Group B. The inhibition test results are shown in Table 5 below.

[0114] Table 5

[0115]

[0116] Conclusion: The above competitive growth inhibition experiment of bacterial flora shows that after co-cultivation of Lactobacillus rhamnosus and Candida albicans, after adding 2% of the private care wash in Experimental Example 3 of the present invention, the concentration of live bacteria of Lactobacillus rhamnosus increased significantly, while the concentration of live bacteria of Candida albicans in mixed culture decreased. The results of statistical analysis show that 2% of the private care wash in Experimental Example 3 of the present invention can promote the growth of Lactobacillus rhamnosus, and at the same time, Lactobacillus rhamnosus, as the dominant flora, can inhibit the growth of Candida albicans.

[0117] The above results of the inhibition experiment on vaginal Gardnerella show that after adding 2% of the private care wash prepared by the composition of the present invention, the concentration of live vaginal Gardnerella increased from 4.69×10 7 CFU / mL was reduced to 2.78×10 5 CFU / mL / 3.19×10 5 CFU / mL, the statistical analysis results show that 2% of the gel of the present invention can inhibit the growth of vaginal Gardnerella.

[0118] Human sensory experiment

[0119] 60 volunteers aged 30 to 70 were selected, including 24 males and 36 females. Volunteer selection criteria: claiming that the private parts feel obvious itching, and usually feel discomfort and irritation with private lotions. They were divided into 4 groups, with 6 males and 9 females in each group. Control Example 1, Control Example 2, Experimental Example 1 and Experimental Example 2 were used daily for four weeks. After four weeks of use in the first phase, the use of 3 axes was stopped, and the results were statistically analyzed after 4 weeks of use in the second phase. This method can detect coincidental reactions or non-coincidental reactions, and this process also indicates possible signs of allergies.

[0120] Evaluation method:

[0121] The volunteers were asked whether they experienced any burning, stinging, or itching sensations.

[0122] - No response +？ Little to no irritation, burning, stinging, itching (+) Mild irritation, burning, stinging, itching + Obvious irritation, burning, stinging, itching NT Untested

[0123] The results are shown in Table 6 below.

[0124] Table 6

[0125]

[0126] Conclusion: Among the volunteers who felt uncomfortable in the private parts, only 2 volunteers thought that they had improved after using the lotion of control example 1; 8 / 15 volunteers were improved after using the lotion of control example 2, and 1 of them felt uncomfortable again in the second test phase. After using the lotions of experimental examples 1 and 2, all 15 volunteers were improved and did not feel uncomfortable symptoms such as itching and burning in the private parts. This shows that the prebiotic formula can improve the discomfort of the private parts of men and women when used in basic cleaning products; and it can enhance its own improvement effect when used in antibacterial products containing antibacterial agents.

[0127] The specific implementation methods described above further illustrate the objectives, technical solutions and beneficial effects of the present invention in detail. It should be understood that the above description is only a specific implementation method of the present invention and is not intended to limit the scope of protection of the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A prebiotic composition for regulating skin flora, characterized in that: It is composed of inulin, α-glucan oligosaccharide, white willow bark extract, and honeysuckle flower extract; In terms of mass percentage, the amount of inulin in the prebiotic composition is 50% to 70%, the amount of α-glucan oligosaccharide is 10% to 30%, the amount of white willow bark extract is 5% to 15%, and the amount of honeysuckle extract is 5% to 20%; The extraction method of the white willow bark extract is: Step 1: The dried bark of the white willow tree is selected, processed, crushed, and filtered to obtain a medicinal powder; Step 2: Add alcohol to the powder, stir in a closed manner, extract and filter at a constant temperature to obtain the first filtrate and the first filter residue; the amount of alcohol added is 3 times that of the powder, and the alcohol concentration is 70%; Step 3: Add alcohol to the first filter residue, stir in a closed manner, extract at a constant temperature and filter to obtain a second filtrate and a second filter residue; the amount of alcohol added is 2.5 times that of the first filter residue, and the alcohol concentration is 70%; Step 4: combining the first filtrate and the second filtrate, standing for more than 12 hours to obtain a supernatant, recovering the alcohol, and filtering the supernatant to obtain a crude extract of white willow bark; Step 5: prepare the crude extract of white willow bark into acid solution, add chloroform, stir in a closed container and let stand, then extract and separate to obtain an upper filtrate and a lower chloroform solution; the pH value of the acid solution is 4.0-5.0; Step 6: Add the upper filtrate into chloroform, stir it in a sealed container, and then let it stand to extract and separate to obtain an upper drug solution, and filter the upper drug solution to obtain the white willow bark extract; The extraction method of the honeysuckle flower extract is: Step 1: Use the dried flowers of honeysuckle as raw materials, select, process, crush and filter them to obtain medicinal powder; Step 2: Add alcohol to the powder, stir in a closed manner, extract and filter at a constant temperature to obtain the first filtrate and the first filter residue; the amount of alcohol added is 3 times that of the powder, and the alcohol concentration is 65%; Step 3: Add alcohol to the first filter residue, stir in a closed manner, extract at a constant temperature and filter to obtain a second filtrate and a second filter residue; the amount of alcohol added is 1.5 times that of the first filter residue, and the alcohol concentration is 65%; Step 4: combining the first filtrate and the second filtrate, standing for more than 12 hours to obtain a supernatant, recovering the alcohol, and filtering the supernatant to obtain a crude honeysuckle extract; Step 5: prepare the crude extract of honeysuckle into acid solution, add chloroform, stir in a closed container and let stand, then extract and separate to obtain an upper filtrate and a lower chloroform solution; the pH value of the acid solution is 5.0-6.0; Step 6: Add the upper filtrate into chloroform, stir in a sealed container and let it stand to obtain an upper medicinal solution after extraction and separation. Filter the upper medicinal solution to obtain the honeysuckle extract.

2. A prebiotic composition for regulating skin flora according to claim 1, characterized in that: By mass percentage, the amount of inulin in the prebiotic composition is 60%, the amount of α-glucan oligosaccharide is 15%, the amount of white willow bark extract is 10%, and the amount of honeysuckle extract is 15%.

3. A prebiotic composition for regulating skin flora according to claim 1, characterized in that: In the extraction method of the white willow bark extract, the constant temperature in step 2 and step 3 is 40° C.; the amount of chloroform added in step 5 is 3 times the amount of the white willow bark extract crude solution; and the amount of chloroform added in step 6 is 3 times the amount of the upper filtrate.

4. A prebiotic composition for regulating skin flora according to claim 1, characterized in that: In the extraction method of the honeysuckle flower extract, the constant temperature in step 2 and step 3 is 60° C.; the amount of chloroform added in step 5 is 3 times that of the crude solution extracted from white willow bark; and the amount of chloroform added in step 6 is 3 times that of the upper filtrate.

5. A method for preparing a prebiotic composition for regulating skin flora according to any one of claims 1 to 4, characterized in that: The willow bark extract and the honeysuckle extract are mixed evenly, inulin is added and stirred evenly, and finally α-glucan oligosaccharide is added and mixed evenly to obtain a prebiotic composition.

6. A prebiotic composition for regulating skin flora according to any one of claims 1 to 4 and a prebiotic composition for regulating skin flora prepared by the preparation method of claim 5 for preparing female private parts wash.

7. A prebiotic composition for regulating skin flora according to any one of claims 1 to 4 and a prebiotic composition for regulating skin flora prepared by the preparation method of claim 5 for preparing male private parts wash.

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