A growth regulator for promoting female and reducing male of chinese chestnut and its preparation method and application

Abstract

The present application relates to the field of Chinese chestnut planting, and in particular, relates to a Chinese chestnut female-promoting and male-reducing growth regulator as well as a preparation method and application thereof. The Chinese chestnut female-promoting and male-reducing growth regulator comprises the following components: paclobutrazol, 6-benzylaminoadenine and a surfactant; wherein the concentration of the paclobutrazol is 20-100 mg / L; the concentration of the 6-benzylaminoadenine is 20-100 mg / L; and the volume concentration of the surfactant is 1%-3%. The Chinese chestnut female-promoting and male-reducing growth regulator is safe and reliable, has obvious effects, is good in stability and easy to operate, can be applied to the production of chestnut farmers, promotes the increase of female flowers and the reduction of male flowers of Chinese chestnut, promotes the differentiation of female flowers of Chinese chestnut, increases the female-male ratio of Chinese chestnut, and obviously increases the yield of a single Chinese chestnut tree.

Description

Technical Field

[0001] This invention relates to the field of chestnut cultivation, and more specifically, to a chestnut growth regulator that promotes female growth and reduces male growth, its preparation method, and its application. Background Technology

[0002] Chestnuts originated in my country and are mainly distributed in mountainous and hilly areas with poor soil. They are an important ecological and economic forest tree species for farmers in mountainous areas of my country to increase their income. my country is a major chestnut producer in the world, but low productivity per unit area is one of the key issues hindering the development of the chestnut industry.

[0003] Chestnuts are monoecious plants with separate male and female flowers. Structurally, their flowers can be divided into purely male inflorescences and mixed inflorescences. Mixed inflorescences contain both mixed male and female flower clusters. The normal ratio of female to male inflorescences in chestnuts is 1:5-10, and the ratio of female to male individual flowers is typically 1:2000-3000. A low number of female flowers and an excessive number of male flowers consuming too much of the tree's nutrients are the main reasons for low yield. Therefore, regulating sex differentiation to increase female flowers and decrease male flowers is the core strategy for improving chestnut yield.

[0004] In view of this, the present invention is hereby proposed. Summary of the Invention

[0005] The primary objective of this invention is to provide a chestnut growth regulator that promotes female growth and reduces male growth. This chestnut growth regulator has synergistic effects among its components, is safe and reliable, has significant effects, good stability, and is easy to operate. It can be applied to chestnut farmers' production to promote female growth and reduce male growth in chestnuts; it promotes the differentiation of female flowers in chestnuts, increases the female-to-male ratio of chestnuts, and significantly increases the yield of a single chestnut tree.

[0006] The second objective of this invention is to provide a method for preparing the aforementioned chestnut growth regulator that promotes female growth and reduces male growth. This method is simple, can be mass-produced, and does not require complex instruments and equipment.

[0007] The third objective of this invention is to provide a method for planting chestnuts, in which the chestnut growth regulator that promotes female growth and reduces male growth is sprayed, resulting in high yield and good quality chestnuts.

[0008] The fourth objective of this invention is to provide an application of the aforementioned chestnut growth regulator that promotes female growth and reduces male growth in chestnut cultivation.

[0009] In order to achieve the above-mentioned objectives of the present invention, the following technical solution is adopted:

[0010] A chestnut growth regulator that promotes female growth and reduces male growth, comprising the following components by weight:

[0011] Paclobutrazol, 6-benzylaminopurine, and surfactants;

[0012] The concentration of paclobutrazol is 20–100 mg / L; the concentration of 6-benzylaminopurine is 20–100 mg / L; and the volume concentration of the surfactant is 1%–3%.

[0013] Preferably, the chestnut growth regulator that promotes female growth and reduces male growth comprises the following components:

[0014] Paclobutrazol, 6-benzylaminopurine, and surfactants;

[0015] The concentration of paclobutrazol is 40–80 mg / L; the concentration of 6-benzylaminopurine is 40–80 mg / L; and the volume concentration of the surfactant is 1.5%–2.5%.

[0016] Preferably, the surfactant includes Tween 20.

[0017] The preparation method of the chestnut growth regulator that promotes female growth and reduces male growth includes the following steps:

[0018] Mix all components thoroughly.

[0019] Preferably, the mixing specifically includes:

[0020] The paclobutrazol and the 6-benzylaminopurine are dissolved in a solution of the surfactant.

[0021] Preferably, the volume concentration of the surfactant solution is 1.5% to 2.5%.

[0022] Preferably, the volume concentration of the surfactant solution is 1.7% to 2.3%.

[0023] A method for cultivating chestnuts, using the aforementioned chestnut growth regulator that promotes female growth and reduces male growth.

[0024] The application of the chestnut growth regulator that promotes female growth and reduces male growth in chestnut cultivation.

[0025] Preferably, the chestnut growth regulator that promotes female growth and reduces male growth is first sprayed during the chestnut budding stage, and then sprayed a second time one week later.

[0026] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0027] (1) The chestnut growth regulator that promotes female growth and reduces male growth provided by the present invention has synergistic effects among its components, is safe and reliable, has obvious effects, good stability, and is easy to operate. It can be applied to chestnut farmers’ production to promote female growth and reduce male growth in chestnuts; promote the differentiation of female flowers in chestnuts, increase the female-to-male ratio of chestnuts, and significantly increase the yield of a single chestnut tree.

[0028] (2) The method for preparing the chestnut growth regulator that promotes female growth and reduces male growth provided by the present invention is simple, can be produced on a large scale, and does not require complicated instruments and equipment.

[0029] (3) The chestnut planting method provided by the present invention, by spraying the chestnut female-promoting and male-reducing growth regulator, results in high chestnut yield and good quality. Attached Figure Description

[0030] To more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the specific embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of the present invention. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0031] Figure 1 This image shows the spraying effect of a growth regulator that promotes female growth and reduces male growth in chestnuts. Detailed Implementation

[0032] The technical solution of the present invention will be clearly and completely described below with reference to the accompanying drawings and specific embodiments. However, those skilled in the art will understand that the embodiments described below are some embodiments of the present invention, but not all embodiments, and are only used to illustrate the present invention, and should not be regarded as limiting the scope of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention. Where specific conditions are not specified in the embodiments, conventional conditions or conditions recommended by the manufacturer shall be followed. Where the manufacturers of reagents or instruments are not specified, they are all conventional products that can be purchased commercially.

[0033] The content of all reagents in the formula of this invention complies with the "National Standard of the People's Republic of China - National Food Safety Standard / Limits of Contaminants in Food" and the "National Standard of the People's Republic of China - Chestnut Quality Grade". The estrogen-promoting agent is stable, safe, convenient to use, and easy to operate.

[0034] A chestnut growth regulator that promotes female growth and reduces male growth includes the following components:

[0035] Paclobutrazol, 6-benzylaminopurine, and surfactants;

[0036] The concentration of paclobutrazol is 20–100 mg / L; the concentration of 6-benzylaminopurine is 20–100 mg / L; and the volume concentration of the surfactant is 1%–3%.

[0037] In some specific embodiments, the concentration of paclobutrazol may be, for example, but not limited to, 20 mg / L, 40 mg / L, 60 mg / L, 80 mg / L or 100 mg / L.

[0038] In some specific embodiments, the concentration of 6-benzylaminopurine may be, for example, but not limited to, 20 mg / L, 40 mg / L, 60 mg / L, 80 mg / L, or 100 mg / L.

[0039] In some specific embodiments, the volume concentration of the surfactant may be, for example, but not limited to, 1%, 1.5%, 2%, 2.5%, or 3%.

[0040] The chestnut growth regulator that promotes female growth and reduces male growth exhibits synergistic effects among its components, is safe and reliable, has significant effects, good stability, and is easy to operate. It can be applied to chestnut farmers' production to promote female growth and reduce male growth in chestnuts; it promotes the differentiation of female flowers in chestnuts, which can increase the female-to-male ratio of chestnuts and significantly increase the yield of a single chestnut tree.

[0041] Paclobutrazol is a plant growth regulator that can delay plant growth, inhibit stem elongation, shorten internodes, promote tillering, increase plant stress resistance, and improve yield.

[0042] The main function of 6-benzylaminopurine is to promote bud formation and induce callus formation. It can be used to improve the quality and yield of tea and tobacco; preserve vegetables and fruits; and cultivate rootless bean sprouts, significantly improving the quality of fruits and leaves.

[0043] Preferably, the chestnut growth regulator that promotes female growth and reduces male growth comprises the following components:

[0044] Paclobutrazol, 6-benzylaminopurine, and surfactants;

[0045] The concentration of paclobutrazol is 40–80 mg / L; the concentration of 6-benzylaminopurine is 40–80 mg / L; and the volume concentration of the surfactant is 1.5%–2.5%.

[0046] Preferably, the surfactant includes Tween 20.

[0047] Tween 20 is an oil-in-water (O / W) emulsifier that can be used as a solubilizer, dispersant, stabilizer, antistatic agent, and lubricant. It is used in mineral oil emulsifiers, cosmetic emulsifiers, pharmaceutical emulsifiers, dye co-solvents, foam plastic stabilizers, photographic emulsion auxiliaries, shampoo viscosity modifiers, antistatic and synergistic agents in spinning oils, and fiber processing auxiliaries. It is particularly suitable for use in conjunction with Span-20. In this invention, it works synergistically with paclobutrazol and 6-benzylaminopurine to promote female growth and reduce male growth in chestnuts, thereby increasing yield.

[0048] The preparation method of the chestnut growth regulator that promotes female growth and reduces male growth includes the following steps:

[0049] Mix all components thoroughly.

[0050] Preferably, the mixing specifically includes:

[0051] The paclobutrazol and the 6-benzylaminopurine are dissolved in a solution of the surfactant.

[0052] Preferably, the volume concentration of the surfactant solution is 1.5% to 2.5%.

[0053] In some specific embodiments, the volume concentration of the surfactant solution may be, for example, but not limited to, 1.5%, 1.7%, 1.9%, 2.1%, 2.3%, or 2.5%.

[0054] Preferably, the volume concentration of the surfactant solution is 0.17% to 2.3%.

[0055] A method for cultivating chestnuts, using the aforementioned chestnut growth regulator that promotes female growth and reduces male growth.

[0056] The application of the chestnut growth regulator that promotes female growth and reduces male growth in chestnut cultivation.

[0057] Preferably, the chestnut growth regulator that promotes female growth and reduces male growth is first sprayed during the chestnut budding stage, and then sprayed a second time one week later.

[0058] The embodiments of the present invention will now be described in detail with reference to specific examples and comparative examples.

[0059] Example 1

[0060] The chestnut growth regulator that promotes female growth and reduces male growth provided in this embodiment includes the following components:

[0061] Paclobutrazol, 6-benzylaminopurine, and surfactants;

[0062] The concentrations of paclobutrazol, 6-benzylaminopurine, and surfactant were 60 mg / L and 2.1% respectively.

[0063] The preparation method of the chestnut growth regulator that promotes female growth and reduces male growth provided in this embodiment includes the following steps:

[0064] 1. First, prepare a Tween 20 solution;

[0065] 2. Add paclobutrazol powder slowly while stirring rapidly until completely dissolved;

[0066] 3. Then add 6-benzylaminopurine and stir until completely dissolved to obtain a white transparent liquid, which is the chestnut estrogen-promoting and androgen-reducing growth regulator.

[0067] The prepared female-promoting and male-reducing growth regulator for chestnuts was sprayed onto the entire plant of the 'Yanshan Zaofeng' variety. The first spray was applied during the budding stage, followed by a second spray one week later. The entire plant was sprayed until the leaves were dripping with water. To avoid photodegradation of the growth regulator, the spraying was done in the evening. The control group was not sprayed with the female-promoting and male-reducing growth regulator.

[0068] Table 1. Effects of the regulator in Example 1 on the yield indicators of chestnut 'Yanshan Zaofeng'

[0069]

[0070] As shown in Table 1, the chestnut growth regulator that promotes female growth and reduces male growth in Example 1 has a significant effect on promoting female growth in chestnuts, increasing the number of female flowers / fruiting branches by 0.78 and the number of chestnut burrs / fruiting branches by 0.72.

[0071] Example 2

[0072] The chestnut growth regulator that promotes female growth and reduces male growth provided in this embodiment includes the following components:

[0073] Paclobutrazol, 6-benzylaminopurine, and surfactants;

[0074] The concentration of paclobutrazol was 20 mg / L, the concentration of 6-benzylaminopurine was 100 mg / L, and the volume concentration of the surfactant was 1.0%.

[0075] The preparation method of the chestnut growth regulator that promotes female growth and reduces male growth provided in this embodiment is the same as in Embodiment 1.

[0076] The prepared female-promoting and male-reducing growth regulator for chestnuts was sprayed onto the chestnut variety 'Jingshuhong'. The first spray was applied during the budding stage, followed by a second spray one week later. The entire plant was sprayed until the leaves dripped with water. To prevent the growth regulator from decomposing in light, the spraying was done in the evening. The control group was not sprayed with the female-promoting and male-reducing growth regulator.

[0077] Table 2. Effects of the regulator in Example 2 on the yield indicators of chestnut 'Jingshuhong'.

[0078]

[0079] As shown in Table 2, the female-promoting and male-reducing growth regulator for chestnuts in Example 2 has a moderate effect on promoting female growth in chestnuts, increasing the number of female flowers / fruiting branches by 0.47 and the number of chestnut burrs / fruiting branches by 0.44.

[0080] Example 3

[0081] The chestnut growth regulator that promotes female growth and reduces male growth provided in this embodiment includes the following components:

[0082] Paclobutrazol, 6-benzylaminopurine, and surfactants;

[0083] The concentration of paclobutrazol was 100 mg / L, the concentration of 6-benzylaminopurine was 20 mg / L, and the volume concentration of the surfactant was 3%.

[0084] The preparation method of the chestnut growth regulator that promotes female growth and reduces male growth provided in this embodiment is the same as in Embodiment 1.

[0085] The prepared female-promoting and male-reducing growth regulator for chestnuts was sprayed onto the chestnut variety 'Jingshuhong'. The first spray was applied during the budding stage, followed by a second spray one week later. The entire plant was sprayed until the leaves dripped with water. To prevent the growth regulator from decomposing in light, the spraying was done in the evening. The control group was not sprayed with the female-promoting and male-reducing growth regulator.

[0086] Table 3 shows the effect of the regulator in Example 3 on the yield standard of chestnut 'Jingshuhong'.

[0087]

[0088] As shown in Table 3, the chestnut female-promoting and male-reducing growth regulator in Example 3 has a moderate effect on promoting female growth in chestnuts, increasing the number of female flowers / fruiting branches by 0.46 and the number of chestnut burrs / fruiting branches by 0.42.

[0089] Example 4

[0090] The chestnut growth regulator that promotes female growth and reduces male growth provided in this embodiment includes the following components:

[0091] Paclobutrazol, 6-benzylaminopurine, and surfactants;

[0092] The concentration of paclobutrazol was 40 mg / L, the concentration of 6-benzylaminopurine was 80 mg / L, and the volume concentration of the surfactant was 2.1%.

[0093] The preparation method of the chestnut growth regulator that promotes female growth and reduces male growth provided in this embodiment is the same as in Embodiment 1.

[0094] The prepared female-promoting and male-reducing growth regulator for chestnuts was sprayed onto the chestnut variety 'Jingshuhong'. The first spray was applied during the budding stage, followed by a second spray one week later. The entire plant was sprayed until the leaves dripped with water. To prevent the growth regulator from decomposing in light, the spraying was done in the evening. The control group was not sprayed with the female-promoting and male-reducing growth regulator.

[0095] Table 4. Effects of the regulator in Example 4 on the number of female flowers in chestnut 'Jingshuhong'.

[0096]

[0097] As shown in Table 4, the female-promoting and male-reducing growth regulator for chestnuts in Example 4 has a moderate effect on promoting female growth in chestnuts, increasing the number of female flowers / fruiting branches by 0.43 and the number of chestnut burrs / fruiting branches by 0.41.

[0098] Example 5

[0099] The chestnut growth regulator that promotes female growth and reduces male growth provided in this embodiment includes the following components:

[0100] Paclobutrazol, 6-benzylaminopurine, and surfactants;

[0101] The concentration of paclobutrazol was 80 mg / L, the concentration of 6-benzylaminopurine was 40 mg / L, and the volume concentration of the surfactant was 1.0%.

[0102] The preparation method of the chestnut growth regulator that promotes female growth and reduces male growth provided in this embodiment is the same as in Embodiment 1.

[0103] The prepared female-promoting and male-reducing growth regulator for chestnuts was sprayed onto the chestnut variety 'Jingshuhong'. The first spray was applied during the budding stage, followed by a second spray one week later. The entire plant was sprayed until the leaves dripped with water. To prevent the growth regulator from decomposing in light, the spraying was done in the evening. The control group was not sprayed with the female-promoting and male-reducing growth regulator.

[0104] Table 5. Effects of the regulator in Example 5 on the number of female flowers in chestnut 'Jingshuhong'.

[0105]

[0106] As shown in Table 5, the female-promoting and male-reducing growth regulator for chestnuts in Example 5 has a moderate effect on promoting female growth in chestnuts, increasing the number of female flowers / fruiting branches by 0.34 and the number of chestnut burrs / fruiting branches by 0.31.

[0107] Comparative Example 1

[0108] The chestnut growth regulator that promotes female growth and reduces male growth provided in this comparative example includes the following components:

[0109] Paclobutrazol, 6-benzylaminopurine, and surfactants;

[0110] The concentration of paclobutrazol was 10 mg / L, the concentration of 6-benzylaminopurine was 120 mg / L, and the volume concentration of the surfactant was 1.0%.

[0111] The preparation method of the chestnut growth regulator that promotes female growth and reduces male growth provided in this comparative example is the same as in Example 1.

[0112] The prepared female-promoting and male-reducing growth regulator for chestnuts was sprayed onto the chestnut variety 'Jingshuhong'. The first spray was applied during the budding stage, followed by a second spray one week later. The entire plant was sprayed until the leaves dripped with water. To prevent the growth regulator from decomposing in light, the spraying was done in the evening. The control group was not sprayed with the female-promoting and male-reducing growth regulator.

[0113] Table 6 shows the effects of the regulators in Comparative Example 1 on the yield indicators of chestnut 'Jingshuhong'.

[0114]

[0115] As shown in Table 6, the chestnut female-promoting and male-reducing growth regulator in Comparative Example 1 had a moderate effect on promoting female growth in chestnuts, increasing the number of female flowers / fruiting branches by 0.27 and the number of chestnut burrs / fruiting branches by 0.23.

[0116] Comparative Example 2

[0117] The chestnut growth regulator that promotes female growth and reduces male growth provided in this comparative example includes the following components:

[0118] Paclobutrazol and surfactants;

[0119] The concentration of paclobutrazol was 120 mg / L and the volume concentration of the surfactant was 1.0%.

[0120] The preparation method of the chestnut growth regulator that promotes female growth and reduces male growth provided in this comparative example is the same as in Example 1.

[0121] The prepared female-promoting and male-reducing growth regulator for chestnuts was sprayed onto the chestnut variety 'Jingshuhong'. The first spray was applied during the budding stage, followed by a second spray one week later. The entire plant was sprayed until the leaves dripped with water. To prevent the growth regulator from decomposing in light, the spraying was done in the evening. The control group was not sprayed with the female-promoting and male-reducing growth regulator.

[0122] Table 7 shows the effects of the regulators in Comparative Example 2 on the yield indicators of chestnut 'Jingshuhong'.

[0123]

[0124] As shown in Table 7, the chestnut female-promoting and male-reducing growth regulator in Comparative Example 2 had a moderate effect on promoting female growth in chestnuts, increasing the number of female flowers / fruiting branches by 0.16 and the number of chestnut burrs / fruiting branches by 0.12.

[0125] Comparative Example 3

[0126] The chestnut growth regulator that promotes female growth and reduces male growth provided in this embodiment does not contain surfactants, compared to that in Example 2.

[0127] The preparation method of the chestnut growth regulator that promotes female growth and reduces male growth provided in this embodiment is the same as in Embodiment 1.

[0128] The prepared female-promoting and male-reducing growth regulator for chestnuts was sprayed onto the chestnut variety 'Jingshuhong'. The first spray was applied during the budding stage, followed by a second spray one week later. The entire plant was sprayed until the leaves dripped with water. To prevent the growth regulator from decomposing in light, the spraying was done in the evening. The control group was not sprayed with the female-promoting and male-reducing growth regulator.

[0129] Table 8 shows the effects of the regulators in Comparative Example 3 on the yield indicators of chestnut 'Jingshuhong'.

[0130]

[0131] As shown in Table 8, the female-promoting and male-reducing growth regulator for chestnuts in Comparative Example 3 had a moderate effect on promoting female growth in chestnuts, increasing the number of female flowers / fruiting branches by only 0.29 and the number of chestnut burrs / fruiting branches by only 0.21.

[0132] Figure 1 The image shows the spraying effect of a female-promoting and male-reducing growth regulator on two chestnut varieties. Figure 1 Image a is a comparison picture of the 'Jingshuhong' chestnut variety; Figure 1 Image b shows the chestnut 'Jingshuhong' after treatment with a female-promoting and male-reducing growth regulator; Figure 1 c is a comparison image of the 'Yanshan Zaofeng' chestnut variety; Figure 1 Image d shows the chestnut 'Yanshan Zaofeng' after treatment with a female-promoting and male-reducing growth regulator. From... Figure 1 The study shows that, under the same conditions of tree vigor, age, and site environment, the female-promoting and male-reducing growth regulator for chestnuts can significantly increase the number of chestnut burrs, resulting in a significant increase in yield.

[0133] Although the present invention has been illustrated and described with specific embodiments, it should be understood that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them; those skilled in the art should understand that modifications can be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some or all of the technical features therein, without departing from the spirit and scope of the present invention; and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention; therefore, this means that all such substitutions and modifications that fall within the scope of the present invention are included in the appended claims.

Claims

1. Application of female-promoting and male-reducing growth regulators in chestnut cultivation; The chestnut growth regulator that promotes female growth and reduces male growth includes the following components: Paclobutrazol, 6-benzylaminopurine, and surfactants; in, The concentration of paclobutrazol is 20-100 mg / L; the concentration of 6-benzylaminopurine is 20-100 mg / L; and the volume concentration of the surfactant is 1%-3%.

2. The application according to claim 1, characterized in that, The chestnut growth regulator that promotes female growth and reduces male growth includes the following components: Paclobutrazol, 6-benzylaminopurine, and surfactants; The concentration of paclobutrazol is 40-80 mg / L; the concentration of 6-benzylaminopurine is 40-80 mg / L; and the volume concentration of the surfactant is 1.5%-2.5%.

3. The application according to claim 1, characterized in that, The surfactant includes Tween 20.

4. The application according to claim 1, characterized in that, The preparation method of the chestnut growth regulator that promotes female growth and reduces male growth includes the following steps: Mix all components thoroughly.

5. The application according to claim 4, characterized in that, The mixing specifically includes: The paclobutrazol and the 6-benzylaminopurine are dissolved in a solution of the surfactant.

6. The application according to claim 5, characterized in that, The volume concentration of the surfactant solution is 1.5% to 2.5%.

7. The application according to claim 5, characterized in that, The volume concentration of the surfactant solution is 1.7% to 2.3%.

8. The application according to claim 1, characterized in that, The chestnut growth regulator that promotes female growth and reduces male growth is first sprayed during the chestnut budding stage, and then sprayed a second time one week later.

9. A method for cultivating chestnuts, characterized in that, Use the chestnut growth regulator that promotes female growth and reduces male growth as described in any one of claims 1 to 8.

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