A suspension sample culture plate

Abstract

The application provides a kind of suspension sample culture plate, including the culture plate main body of closed rectangular shell structure;The culture plate main body is arranged along horizontal direction, and its inside is set to culture area and liquid change area from top to bottom;Culture isolation layer is arranged between the culture area and the liquid change area;The end of the culture plate main body corresponding to the culture area and the liquid change area is provided with interface.The device provided by the application meets the special requirements of suspension sample culture by setting the culture plate main body in a closed state and installing the interface on the culture plate main body, which meets the requirement of sealing state, at the same time, the device can meet the requirement of not polluting the experimental environment during the suspension sample culture process, and the device can be used to culture different sizes and different types of suspension samples, and through the separation of culture area and liquid change area, the culture solution can be directly replaced as needed during the suspension sample culture process, which is simple to operate, and the suspension sample is not lost during the replacement of culture solution.

Description

Technical Field

[0001] This invention relates to the technical field of cell culture plates applied in research fields such as space life science, aerospace medicine and cell biology, and specifically to a suspension sample culture plate. Background Technology

[0002] Research in fields such as space life sciences, aerospace medicine, and cell biology involves the culture of numerous different types of suspended samples, including cells, tissues, organoids, bacteria, and algae. Currently, the conventional culture method uses standard cell culture flasks or petri dishes. However, these standard culture materials have significant limitations in the culture of suspended samples:

[0003] Different types of samples require regular and quantitative replacement of the culture medium during culture. Direct replacement of the culture medium results in the loss of suspended samples. To minimize the loss of suspended samples during conventional culture, the samples need to be removed from the culture medium and centrifuged, then the centrifuged samples are added to new culture medium and resuspended for reculture. This process involves multiple steps, carries a high risk of sample contamination, and centrifugation inevitably causes damage to the samples.

[0004] Suspended samples are cultured using conventional culture materials. Sample collection requires centrifugation, which is inconvenient and requires the assistance of peripheral experimental equipment.

[0005] Moreover, some existing conventional culture supplies do not have inlet / outlet connectors or have open structures that are not sealed, making it difficult to meet the special requirements for sealed suspension sample culture, such as in space experiments, dynamic experiments, or automated culture. Summary of the Invention

[0006] To address the problems of numerous operational steps, high risk of sample contamination, and unavoidable damage to samples during existing culture medium replacement processes, this invention provides a suspension sample culture plate, comprising a culture plate body with a closed rectangular shell structure.

[0007] The culture plate body is arranged horizontally, and its interior is divided into a culture area and a medium exchange area from top to bottom;

[0008] A culture isolation layer is provided between the culture zone and the medium exchange zone;

[0009] An interface is provided at one end of the culture plate body corresponding to the culture zone and the medium exchange zone.

[0010] Preferably, the fluid exchange zone is provided with a plurality of baffles arranged in parallel;

[0011] The baffle extends along the extension direction of the culture plate body and is installed vertically on the inner bottom wall of the culture plate body.

[0012] Preferably, the length of the baffle is less than the extension length of the culture plate body.

[0013] Preferably, the culture isolation layer includes a culture isolation membrane arranged in a horizontal direction and a plate-shaped separator layer;

[0014] The separator layer is disposed on the upper side of the baffle and is fixedly installed on the middle of the inner side of the culture plate body;

[0015] The culture isolation membrane is laid on the upper side of the baffle and is coplanar with the separation layer.

[0016] Preferably, the upper side of the culture area is provided as a cover-like culture bag;

[0017] The culture bag is sealed and installed on the upper side of the culture plate body.

[0018] Preferably, the end where the fluid exchange zone is located is provided with at least two interfaces, namely a fluid inlet interface and a fluid outlet interface.

[0019] Preferably, the liquid inlet interface is threadedly connected to a liquid inlet connector;

[0020] The liquid outlet interface is threadedly connected to a liquid outlet connector.

[0021] Preferably, both the inlet connector and the outlet connector are threaded with connector caps.

[0022] Preferably, the interface at the end where the culture area is located is equipped with a suspended sample injection connector;

[0023] When collecting suspended samples in the culture area, a collection connector is installed on the suspended sample injection connector.

[0024] Preferably, the collection connector includes a cylindrical outer shell, a damping filter block, an outer interface, and an inner interface;

[0025] The damping filter block is interference-fitted into the cylindrical housing and is coaxial with the cylindrical housing;

[0026] The external interface and the internal interface are respectively fixedly disposed at both ends of the columnar shell, and both communicate with the inner cavity of the columnar shell;

[0027] The internal interface is connected to the interface at the end where the culture area is located.

[0028] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0029] This invention provides a suspension sample culture plate, comprising a culture plate body with a closed rectangular shell structure; the culture plate body is arranged horizontally, and its interior is divided into a culture zone and a medium exchange zone from top to bottom; a culture isolation layer is provided between the culture zone and the medium exchange zone; an interface is provided at one end of the culture plate body corresponding to the culture zone and the medium exchange zone. The device provided by this invention, by setting up a closed culture plate body and installing interfaces on the culture plate body, meets the special requirements for the culture of suspension samples requiring a sealed state. Simultaneously, this device can meet the requirement of not causing pollution to the experimental environment during the culture of suspension samples. Furthermore, this device can be used to culture suspension samples of different sizes and types. Moreover, by separating the culture zone and the medium exchange zone, the culture medium can be directly replaced as needed during the culture of suspension samples, making the operation simple and preventing loss of suspension samples during culture medium replacement. Attached Figure Description

[0030] Figure 1 This is a schematic diagram of the overall structure of the suspension sample culture plate of the present invention;

[0031] Figure 2 This is a schematic diagram of the structure of the culture plate body of the present invention;

[0032] Figure 3 This is a schematic diagram of the structure of the collection connector of the present invention;

[0033] Figure 4 This is a schematic diagram of the structure of the present invention, showing the separation of the suspended sample injection connector and the collection connector;

[0034] Figure 5 This is a schematic diagram of the assembly of the suspended sample injection connector and the collection connector of the present invention.

[0035] The components are as follows: 1. Culture plate body; 2. Culture zone; 3. Medium exchange zone; 4. Baffle; 5. Culture isolation membrane; 6. Separator layer; 7. Culture soft bag; 8. Connector cap; 9. Collection connector; 10. Columnar shell; 11. Damping filter block; 12. External interface; 13. Internal interface. Detailed Implementation

[0036] To better understand this invention, the following description, in conjunction with the accompanying drawings and examples, will further illustrate the invention.

[0037] This invention provides a suspension sample culture plate. By setting up a closed culture plate body and installing an interface on the culture plate body, it meets the special requirements of culturing suspension samples in a sealed state. At the same time, this device can meet the requirement of not causing pollution to the experimental environment during the culture of suspension samples. Moreover, this device can be used to culture suspension samples of different sizes and types. Furthermore, by separating the culture zone and the medium replacement zone, the culture medium can be directly replaced as needed during the culture of suspension samples. The operation is simple and the suspension samples are not lost when the culture medium is replaced.

[0038] Example:

[0039] A suspension sample culture plate, such as Figure 1 and Figure 2 As shown, the culture plate body 1 includes a closed rectangular shell structure. The culture plate body 1 is arranged horizontally, and its interior is divided into a culture zone 2 and a medium exchange zone 3 from top to bottom. A culture isolation layer is provided between the culture zone 2 and the medium exchange zone 3. An interface is provided at one end of the culture plate body 1 corresponding to the culture zone 2 and the medium exchange zone 3. The function of the culture isolation layer is to divide the culture plate body 1 into upper and lower parts, namely, the culture zone 2 and the medium exchange zone 3 located on the upper side. The culture medium in the medium exchange zone 3 can be continuously refreshed, and nutrients in the fresh culture medium diffuse through the culture isolation layer to the cell culture zone 2, achieving the goal of direct medium exchange during suspension sample culture. Simultaneously, the interface can be connected to other devices, such as inlet and outlet pipes, to prevent contamination of the experimental environment when using the suspension sample culture plate.

[0040] The medium replacement zone 3 is equipped with multiple parallel baffles 4. The baffles 4 extend along the extension direction of the culture plate body 1 and are installed vertically on the inner bottom wall of the culture plate body 1. In this embodiment, the length of the baffle 4 is shorter than the extension length of the culture plate body 1. One end of the baffle 4 is fixedly connected to the end of the culture plate body 1 with an interface, and the other end has a gap with the end of the culture plate body 1 opposite to the interface. When changing the medium, the new nutrient solution can flow through the gap, and the used nutrient solution can flow through the gap and be discharged, forming a cycle.

[0041] The culture isolation layer includes a horizontally arranged culture isolation membrane 5 and a plate-shaped separator layer 6. The separator layer 6 is located on the upper side of the baffle 4 and is fixedly installed in the middle of the inner side of the culture plate body 1. The culture isolation membrane 5 is laid on the upper side of the baffle 4 and is coplanar with the separator layer 6. The separator layer 6 is located above the baffle 4 and at one end of the culture plate body 1, and is welded to the baffle 4 and the culture plate body 1 using a high-frequency welding process. The culture isolation membrane 5 is located above the baffle 4 and at the end of the culture plate body 1 near the joint, and is adhered to the upper side of the baffle 4 and the inner side of the culture plate body 1 using biological-grade double-sided adhesive.

[0042] The upper side of the culture area 2 is provided with a cover-like culture bag 7; the culture bag 7 is sealed and installed on the upper side of the culture plate body 1. In this embodiment, the culture bag 7 is located on the upper part of the separator layer 6 and the culture isolation membrane 5, and its periphery is heat-sealed around the separator layer 6 and the culture isolation membrane 5 of the culture plate body 1 to form a suspended sample culture area 2.

[0043] At least two interfaces are provided at the end of the medium replacement zone 3: an inlet interface and an outlet interface. The inlet interface is threaded with an inlet connector; the outlet interface is threaded with an outlet connector. The inlet and outlet connectors are responsible for the entry and exit of the culture medium in the medium replacement zone 3, respectively. When new nutrient solution enters through the inlet interface via the inlet connector, the old nutrient solution in the culture plate body 1 flows through the gap along with the injection of new nutrient solution, thereby allowing the old nutrient solution to be discharged through the outlet connector, thus achieving the replacement of the nutrient solution.

[0044] Both the inlet and outlet connectors are threaded with connector caps 8. The function of connector caps 8 is to protect the connectors during sample culture in the culture plate, prevent reagent leakage, and maintain the sterility of the connectors. They are tightened onto the connectors by screwing them on.

[0045] An interface located at the end of the culture zone 2 is equipped with a suspended sample injection connector; when collecting suspended samples within the culture zone 2, a collection connector 9 is installed on the suspended sample injection connector. The suspended sample injection connector is used for injecting suspended samples into the culture zone 2, and the collection connector 9 is used for collecting suspended samples within the culture zone 2. In this embodiment, the collection connector 9 is installed on the suspended sample injection connector.

[0046] like Figure 3 As shown, the collection connector 9 includes a cylindrical shell 10, a damping filter block 11, an outer interface 12, and an inner interface 13. The damping filter block 11 is interference-fitted into the cylindrical shell 10 and is coaxial with the cylindrical shell 10. The outer interface 12 and the inner interface 13 are respectively fixed at both ends of the cylindrical shell 10 and are both connected to the inner cavity of the cylindrical shell 10. The inner interface 13 is connected to the suspended sample injection connector. After the collection connector 9 is responsible for collecting the suspended sample, the damping filter block 11 is interference-fitted into one end of the shell, and the interface is installed onto the culture plate body 1 using a hot-melt process. The collection connector 9 is threaded to the interface at the end where the culture zone 2 is located. A screw-in syringe is installed at the other end of the collection connector 9. By pulling the syringe, when the suspended sample and culture medium in the culture zone 2 flow through the damping filter block 11, the suspended sample is trapped, the culture medium is drawn into the syringe after passing through the damping filter block 11, and the suspended sample is retained in the collection connector 9 for subsequent processing.

[0047] This invention provides a suspension sample culture plate, such as... Figure 4 and Figure 5As shown, it can be used to culture suspension samples of different sizes and types, such as suspended cells, tissue blocks, organoid clumps, bacteria, and various algae. While supporting the culture of multiple types of suspension samples, it can also achieve non-destructive online culture medium updates for suspension samples. The configured sample collection connector 9 enables convenient and efficient collection of cultured suspension samples. Its closed structure with connectors meets the special requirements for suspension sample culture under sealed conditions. The configured interface allows for manual medium replacement and sample collection, and can also be connected to an automatic medium replacement pipeline to achieve the goal of automatic medium replacement culture.

[0048] The specific structure of the suspension sample culture plate provided in this embodiment is as follows:

[0049] The main body 1 of the culture plate is made of polystyrene injection molding, which can ensure the transparency of the culture plate to meet the requirements of sample observation, and also ensure the biocompatibility of the materials required for cell culture. The main body 1 of the culture plate is 80mm long, 38mm wide and 10mm high, with the lower medium exchange area 3 being 5mm high and the baffle 4 being 1mm thick.

[0050] The separator layer 6 is machined from 1mm polystyrene sheet and welded to one end of the culture plate body 1, above the baffle 4, using a high-frequency heat sealing process. The culture isolation membrane 5 is a hydrophilic porous membrane made of 20um nylon material. After being cut to a suitable size, it is fixed to the baffle 4 with bio-particle double-sided tape, and together with the separator layer 6, it divides the culture plate into two non-directly connected upper and lower parts.

[0051] The culture bag 7, made of EVA material, is cut and molded, and then heat-sealed onto the culture isolation membrane 5 and the separator layer 6 to form a 10ml volume three-dimensional soft suspended sample culture space. The edges around the upper part of the culture plate body 1 protect the culture bag 7.

[0052] The inlet and outlet connectors are standard polypropylene Luer connectors with 1 / 4-28 threaded connections. They are connected to the threaded holes on the culture plate body 1 via threads, with an internal hole diameter of 2mm.

[0053] The connector cap 8 can be a standard internal spiral Luer plug made of polypropylene.

[0054] The outer shell and interface of the collection connector 9 are made of polystyrene injection molding. The outer interface 12 is a Luer female connector and the inner interface 13 is a Luer male connector. The internal damping filter block 11 is a nylon powder compressed filter block with a filter pore size of 20um, which is pressed into the outer shell with an interference fit.

[0055] Its specific implementation method:

[0056] Using a 10ml screw-type syringe, inject culture medium containing the suspended sample into culture zone 2 of the culture plate through the suspension sample injection connector, and tighten the connector cap 8. Then, using the screw-type syringe, inject fresh culture medium into the medium replacement zone 3 through an inlet connector. After the medium replacement zone 3 is full of culture medium, remove the syringe and screw connector caps 8 onto both the inlet and outlet connectors. The culture plate can then be placed in a cell culture incubator to culture the sample.

[0057] When the culture medium needs to be changed, remove the connector caps 8 from both the inlet and outlet connectors. Use a screw-type syringe to draw 10 ml of fresh culture medium and connect it to the inlet connector. Push the fresh culture medium into the medium replacement zone 3. The original culture medium in the replacement zone 3 will flow out from the outlet connector. After the medium replacement is complete, remove the syringe, screw the connector cap 8 back on, and the suspension sample can be cultured again. The components of the fresh culture medium in the replacement zone 3 will diffuse through the culture isolation membrane 5 to the culture zone 2 due to the concentration difference, providing the necessary nutrients for sample growth.

[0058] After the experiment, remove the suspended sample injection connector and connect the collection connector 9. Connect the empty screw-in syringe to the other end of the collection connector 9, draw the syringe, and inject the culture medium in the culture zone 2 into the syringe through the damping filter block 11 in the collection connector 9. The suspended sample is retained in the collection connector 9 and can be used for subsequent processing.

[0059] Obviously, the described embodiments are only some, not all, of the embodiments of the present invention. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without inventive effort are within the scope of protection of the present invention.

[0060] The above are merely embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention are included within the scope of the claims of the present invention pending approval.

Claims

1. A suspension sample culture plate, characterized in that, The culture plate body (1) includes a closed rectangular shell structure; The culture plate body (1) is arranged in a horizontal direction, and its interior is divided into a culture area (2) and a medium exchange area (3) from top to bottom; A culture isolation layer is provided between the culture zone (2) and the medium exchange zone (3); An interface is provided at one end of the culture plate body (1) corresponding to the culture zone (2) and the liquid exchange zone (3); The fluid exchange zone (3) is provided with multiple parallel baffles (4); The baffle (4) extends along the extension direction of the culture plate body (1) and is installed vertically on the inner bottom wall of the culture plate body (1); The length of the baffle (4) is less than the extension length of the culture plate body (1); The culture isolation layer includes a culture isolation membrane (5) arranged in a horizontal direction and a plate-shaped partition layer (6); The separator layer (6) is disposed on the upper side of the baffle (4) and is fixedly installed on the middle of the inner side of the culture plate body (1); The culture isolation membrane (5) is laid on the upper side of the baffle (4) and is coplanar with the separation layer (6); The interface at the end of the culture area (2) is equipped with a suspended sample injection connector; When collecting suspended samples in the culture area (2), a collection connector (9) is installed on the suspended sample injection connector; The collection connector (9) includes a cylindrical shell (10), a damping filter block (11), an outer interface (12), and an inner interface (13); The damping filter block (11) is interference-fitted into the cylindrical shell (10) and is coaxial with the cylindrical shell (10); The external interface (12) and the internal interface (13) are respectively fixedly disposed at both ends of the columnar shell (10), and both are in communication with the inner cavity of the columnar shell (10); The inner interface (13) is connected to the interface at the end where the culture area (2) is located.

2. The suspension sample culture plate according to claim 1, characterized in that, The upper side of the culture area (2) is provided with a cover-like culture bag (7); The culture bag (7) is sealed and installed on the upper side of the culture plate body (1).

3. The suspension sample culture plate according to claim 1, characterized in that, The end of the fluid exchange zone (3) is provided with at least two interfaces, namely a fluid inlet interface and a fluid outlet interface.

4. A suspension sample culture plate according to claim 3, characterized in that, The inlet port is threaded with an inlet connector; The liquid outlet interface is threaded with a liquid outlet connector.

5. A suspension sample culture plate according to claim 4, characterized in that, Both the inlet connector and the outlet connector are threaded with connector caps (8).

Images