A phyllotreta striolata attractant and phyllotreta striolata lure

Abstract

The application discloses a phyllotreta striolata attractant formed by diluting verbenone and / or verbenol in n-hexane to 10 mg / L-1000 mg / L. The application also discloses a phyllotreta striolata attractant comprising active components in the following weight parts: verbenone and / or verbenol 30-100 parts, butyl isothiocyanate 0-25 parts, allyl isothiocyanate 0-25 parts. The application further discloses a phyllotreta striolata lure core. The application provides the use of verbenone and verbenol as components of the phyllotreta striolata attractant, which has the characteristics of simple operation, convenient use, high attraction efficiency, etc.; the attraction rate of the phyllotreta striolata attractant to adult phyllotreta striolata is higher than 80%, the number of adult phyllotreta striolata in the field is significantly reduced, and the insect population density is reduced; the popularization of the application will significantly reduce the use of chemical pesticides, improve the problem of serious phyllotreta striolata damage, and improve the quality of cruciferous vegetables.

Description

Technical Field

[0001] This invention belongs to the field of biological control technology, and in particular relates to a flea beetle attractant and a flea beetle lure. Background Technology

[0002] The yellow-striped flea beetle (Phyllotreta striolata) is a global pest widely distributed in my country, found in all provinces, especially in the southern regions where it is particularly severe, surpassing the diamondback moth to become the number one pest in vegetable production. The yellow-striped flea beetle damages a variety of plants, especially cruciferous vegetables, primarily mustard greens, cabbage, radish, kale, cauliflower, rapeseed, and other cruciferous vegetables, as well as other crops containing glucosinolates. Both larvae and adults of the yellow-striped flea beetle damage vegetables. Larvae damage the root system, causing root rot; newly emerged seedlings often die after being damaged. Adults feed on the tender stems and leaves, creating holes and notches in the leaves, severely affecting vegetable quality and leading to significant economic losses.

[0003] For a long time, the control of the striped flea beetle has mainly relied on chemical agents, which has led to the beetle developing resistance to pesticides, reducing the effectiveness of control, and causing many environmental and social problems. With people's increasing awareness of ecology, environment, and food safety, reducing the amount of chemical pesticides used and developing "green pest control" strategies has become a strategic goal for the development of plant protection science.

[0004] Currently, chemical control of the striped flea beetle mainly relies on aggregation pheromones, the main component of which is (+)-(6R,7S)-himachala-9,11-diene. However, the synthesis process of these pheromones is complex and costly, making them difficult to promote. Verbenone and verbenasol are aggregation pheromones for many forestry pests and are commonly used as repellents for these pests both domestically and internationally. Although the striped flea beetle and many other forestry pests belong to the order Coleoptera, verbenaone, verbenasol, and similar components have not been detected in the pheromone composition of the striped flea beetle. Therefore, there are currently no reports on the research and application of verbenaone and verbenasol in the striped flea beetle and other vegetable pests. Summary of the Invention

[0005] The purpose of this invention is to provide the application of verbenatenone and verbenatenol as attractants for the yellow-striped flea beetle, aiming to provide a yellow-striped flea beetle control attractant that is highly attractive, environmentally friendly, and safe for humans and animals.

[0006] The technical solution adopted by the present invention to solve its technical problem is: a flea attractant for yellow striped flea beetles, which is formed by diluting verbenae and / or verbenae alcohol in n-hexane to 10 mg / L-1000 mg / L.

[0007] Further, it is formed by diluting verbenaone and / or verbenasol in n-hexane to 10 mg / L, or to 100 mg / L, or to 1000 mg / L.

[0008] The present invention also discloses a flea beetle attractant, comprising the following active components in parts by weight: verbena ketone and / or verbena ketol 30-100 parts, butyl isothiocyanate 0-25 parts, and allyl isothiocyanate 0-25 parts.

[0009] Furthermore, it also includes plant-derived fragrances; the active ingredient has a weight of 100-300 mg, and the fragrance has a weight of 0.1-0.3 mL.

[0010] Furthermore, the flavoring is one or two of lemongrass flavoring, mustard oil flavoring, and floral flavoring.

[0011] The present invention also discloses a yellow flea beetle attractant, comprising a capillary tube sealed at both ends, wherein the capillary tube is encapsulated with the aforementioned yellow flea beetle attractant.

[0012] Furthermore, the capillary is made of one or both of polyethylene and polypropylene plastics.

[0013] Furthermore, the wall thickness of the capillary is 0.2 to 0.25 mm.

[0014] The present invention also discloses the application of attractants in attracting yellow-striped flea beetles.

[0015] This invention also discloses the application of attractants in lures for attracting yellow-striped flea beetles.

[0016] The beneficial effects of this invention are as follows: The verbenacenone and verbenacol provided by this invention, as components of the yellow-striped flea beetle attractant, are simple to operate, convenient to use, and have high attraction efficiency; the attraction rate for adult yellow-striped flea beetles is higher than 80%, significantly reducing the number of adult yellow-striped flea beetles in the field and lowering the insect population density; the promotion of this invention will significantly reduce the use of chemical pesticides, improve the serious problem of yellow-striped flea beetle damage, and improve the quality of cruciferous vegetables; this invention has broad economic, social, and ecological benefits in the green control of yellow-striped flea beetles; verbenacenone and verbenacol are natural compounds with stable structures, are non-toxic and pollution-free, providing a new approach for environmentally friendly and efficient control of yellow-striped flea beetle damage to cruciferous vegetables, which is of great significance for the control of flea beetles in vegetables in my country and has broad economic, social, and ecological benefits. Attached Figure Description

[0017] Figure 1 This is a schematic diagram of the Y-shaped olfactory sensor in this invention.

[0018] Figure 2This invention provides an analysis of the attraction results of the yellow-striped flea beetle.

[0019] Figure 3 This invention provides an analysis of the attraction results of the yellow-striped flea beetle. Detailed Implementation

[0020] To enable those skilled in the art to better understand the present invention, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of the present invention, and not all of them. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort should fall within the scope of protection of the present invention.

[0021] A flea attractant is prepared by diluting verbena in hexane to a concentration of 10 mg / L-1000 mg / L. More specifically, it is prepared by diluting verbena in hexane to a concentration of 10 mg / L, 100 mg / L, or 1000 mg / L.

[0022] A flea attractant is prepared by diluting verbenacol in hexane to a concentration of 10 mg / L to 1000 mg / L. More specifically, it is prepared by diluting verbenacol in hexane to a concentration of 10 mg / L, 100 mg / L, or 1000 mg / L.

[0023] A flea attractant for yellow-striped flea beetles is formed by diluting verbenae and verbenaenool in hexane to a concentration of 10 mg / L-1000 mg / L, wherein the ratio of verbenae to verbenaenool is 1:1.

[0024] A flea beetle attractant comprises the following active components in parts by weight: verbenacenone and / or verbenaenool 30-100 parts, butyl isothiocyanate 0-25 parts, and allyl isothiocyanate 0-25 parts. This invention does not specify the source of verbenacenone, verbenaenool, butyl isothiocyanate, and allyl isothiocyanate; commercially available products are acceptable.

[0025] The flea beetle attractant of the present invention comprises the above-mentioned active component and a plant-extracted fragrance. The active component weighs 100-300 mg, and the fragrance is 0.1-0.3 mL, wherein the fragrance is one or two selected from lemongrass fragrance, mustard oil fragrance, and floral fragrance. Mixing the active composition with the fragrance yields the flea beetle attractant.

[0026] The attractant for *Stropharia flavomarginata* is loaded into a capillary tube, which constitutes the attractant core of this invention. The capillary tube is made of one or both of polyethylene and polypropylene plastic. The wall thickness of the capillary tube is 0.2–0.25 mm. This invention does not have a specific limitation on the amount of encapsulated *Stropharia flavomarginata* attractant used; the amount of encapsulated attractant can be determined based on the attraction time for *Stropharia flavomarginata*.

[0027] The yellow-striped flea beetle lure can be used alone or in combination with yellow sticky traps to release yellow-striped flea beetle attractants and trap adult yellow-striped flea beetles.

[0028] This invention provides the use of verbenaconin and verbenacol as attractant components for flea beetles. To verify that verbenaconin or verbenacol has an attractant effect on flea beetles, the following experiments were conducted.

[0029] use Figure 1 The Y-type olfactory instrument shown was used to measure the behavioral response of adult yellow-striped flea beetles to verbenatenone or verbenatenol.

[0030] The Y-shaped olfactory device is made of glass, with a main arm 12cm long and an inner diameter of 1.5cm; the two side arms are 10cm long and 1.5cm in inner diameter, with an included angle of 75°. The Y-shaped olfactory device consists of, in order: an air sampler, an activated carbon tube, a humidifier bottle, a flow meter, a odor source bottle, and a Y-shaped tube, with all components connected by polytetrafluoroethylene tubing.

[0031] The attractant for the yellow-striped flea beetle was prepared into solutions with concentrations of 10 mg / L, 100 mg / L, and 1000 mg / L using hexane. 10 μL of each solution was dropped onto a 20 × 30 mm filter paper strip. After the solvent evaporated, the filter paper strip was placed in the odorant bottle, and aeration was maintained for 2 minutes at a flow rate of 200 mL / min to facilitate compound evaporation. One starved yellow-striped flea beetle was placed into the main arm of a Y-shaped tube to observe its selection behavior. Each flea beetle was observed for 2 minutes; those that entered the end of the selection arm were considered selected, otherwise, they were considered unselected. Each group consisted of 20 adult yellow-striped flea beetles, and each group was repeated 3 times. The positions of the two arms of the Y-shaped tube were rotated every 10 beetles tested to eliminate the influence of ambient light and tube arm position on the selection behavior of the flea beetles. After each test, the tube was thoroughly cleaned with alcohol and dried before proceeding to the next test.

[0032] Selection rate (%) = (Total number of insects in the odor arm or total number of insects in the control arm / Total number of insects tested) × 100.

[0033] Data analysis: Experimental data were analyzed using SPSS 19.0 statistical software. The chi-square test was used to compare the significance of the behavioral responses of adult yellow-striped flea beetles to verbenatenone or verbenatenol.

[0034] The results analysis is as follows: Figure 2At a concentration of 10 mg / L, verbenazone attracted 81.7% of adult flea beetles. At a concentration of 100 mg / L, the attraction rate reached 76.7%. At a concentration of 1000 mg / L, the attraction rate reached 73.8%. The results indicate that verbenazone has a significant attractant effect on adult flea beetles within its concentration range.

[0035] The results analysis is as follows: Figure 3 At a concentration of 10 mg / L, verbenafil showed an attraction rate of 82.5% for adult flea beetles. At 100 mg / L, the attraction rate was 77.5%. At 1000 mg / L, the attraction rate was 75.8%. These results indicate that verbenafil has a significant attraction effect on adult flea beetles within its concentration range.

[0036] Example 1

[0037] A flea attractant composed solely of verbenae, at a concentration of 10 mg / L-1000 mg / L.

[0038] Example 2

[0039] A flea attractant composed solely of verbenaenoyl alcohol at a concentration of 10 mg / L-1000 mg / L.

[0040] Example 3

[0041] A flea attractant for yellow-striped flea beetles, comprising the following components in parts by weight: 50 parts verbenacenone and 50 parts verbenacenol.

[0042] Example 4

[0043] A flea attractant for yellow striped flea beetles, comprising the following components in parts by weight: 50 parts verbenaconin and 50 parts butyl isothiocyanate.

[0044] Example 5

[0045] A flea attractant for yellow striped flea beetles, comprising the following components in parts by weight: 50 parts verbenaenoyl alcohol and 50 parts butyl isothiocyanate.

[0046] Example 6

[0047] A flea attractant for yellow striped flea beetles, comprising the following components in parts by weight: 50 parts verbena, 25 parts butyl isothiocyanate, and 25 parts allyl isothiocyanate.

[0048] Example 7

[0049] A flea attractant for yellow striped flea beetles, comprising the following components in parts by weight: 50 parts verbenaenoyl alcohol, 25 parts butyl isothiocyanate, and 25 parts allyl isothiocyanate.

[0050] Example 8

[0051] A flea attractant for yellow striped flea beetles, comprising the following components in parts by weight: 30 parts verbenatenone, 30 parts verbenatenol, 15 parts butyl isothiocyanate, and 15 parts allyl isothiocyanate.

[0052] Example 9

[0053] A flea attractant for yellow striped flea beetles, comprising the following components in parts by weight: 39 parts verbenacenone, 39 parts verbenacenol, 2 parts butyl isothiocyanate, and 20 parts allyl isothiocyanate.

[0054] Example 10

[0055] Dissolve 25 mg of the active composition of Examples 1-9 of this invention in n-hexane, add 0.1-0.3 mL of fragrance to obtain a yellow flea beetle attractant. Load the prepared yellow flea beetle attractant into a capillary tube as a yellow flea beetle lure. The capillary tube is 8 mm long, with an inner diameter of 1.3-1.4 mm, an outer diameter of 1.3-1.4 mm, and a wall thickness of 0.2-0.25 mm.

[0056] Example 11

[0057] To further illustrate the feasibility of the yellow-striped flea beetle attractant provided by this invention, an experiment on the trapping effect of the yellow-striped flea beetle attractant was conducted at Nuantian Agricultural Co., Ltd., Wucheng District, Jinhua City, Zhejiang Province, from April 23 to June 15, 2023.

[0058] A uniformly growing Shanghai bok choy field was selected as the experimental field. Different formulations of flea beetle lures obtained in Example 10 were fixed in yellow sticky traps to trap adult flea beetles. Six points were randomly and evenly set up for each formulation of flea beetle attractant. The number of adult flea beetles trapped was investigated every two days for six consecutive days.

[0059] The experimental results are as follows:

[0060] The attraction effects of different formulations of flea beetle attractants on adult flea beetles.

[0061] Implementation Cases Average number of insects attracted Implementation Case 1 502 Implementation Case 2 478 Implementation Case 3 534 Implementation Case 4 602 Implementation Case 5 586 Implementation Case 6 611 Implementation Case 7 630 Implementation Case 8 832 Implementation Case 9 928 Comparison of Implementation Cases 262

[0062] The comparative implementation case used a decoy prepared with a solvent control (n-hexane) and attached to a yellow plate to create a trapping device.

[0063] The experimental results show that the flea beetle trapping capacity provided by this invention is significantly higher than that of the comparative examples. Among them, examples 8 and 9 show the best trapping effect for flea beetles.

[0064] This yellow-striped flea beetle attractant is highly specific, has a strong attraction effect, does not induce resistance, does not harm natural enemies, is harmless to humans and animals, is environmentally friendly, and is simple to prepare and use.

[0065] The above specific embodiments are used to explain and illustrate the present invention, but not to limit the present invention. Any modifications and changes made to the present invention within the spirit and scope of the claims shall fall within the protection scope of the present invention.

Claims

1. A yellow-striped flea beetle attractant, characterized in that: It is formed by diluting verbenatenone and verbenatenol in hexane to a concentration of 10 mg / L-1000 mg / L.

2. The flea beetle attractant according to claim 1, characterized in that: It is formed by diluting verbenaone and verbenaenool in hexane to 10 mg / L, or to 100 mg / L, or to 1000 mg / L.

3. A flea beetle attractant, characterized in that, The active ingredients include the following parts by weight: verbenatenone and / or verbenatenol 30-100 parts, butyl isothiocyanate 2-25 parts, and allyl isothiocyanate 15-25 parts.

4. The flea beetle attractant according to claim 3, characterized in that: It also includes plant-derived fragrances; the active ingredient has a weight of 100-300 mg, and the fragrance has a weight of 0.1-0.3 mL.

5. The flea beetle attractant according to claim 4, characterized in that: The flavoring is one or two of lemongrass flavoring, mustard oil flavoring, and floral flavoring.

6. A yellow-striped flea beetle lure, characterized in that: It includes a capillary tube sealed at both ends, wherein the capillary tube contains the yellow striped flea attractant as described in any one of claims 1-5.

7. The yellow-striped flea beetle decoy according to claim 6, characterized in that: The capillary is made of one or both of polyethylene and polypropylene plastics.

8. The yellow-striped flea beetle decoy according to claim 6, characterized in that: The capillary has a wall thickness of 0.2 to 0.25 mm.

9. The use of the attractant according to any one of claims 1 to 5 in attracting flea beetles.

10. The use of the lure according to any one of claims 6 to 8 in attracting yellow-striped flea beetles.

11. Application of verbenatenone and / or verbenatenol in the preparation of attractants for flea beetles.

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