Uses of L-histidine in the preparation of products that increase the number of implantation sites in animal embryos.

Abstract

This invention belongs to the field of animal nutrition technology, and specifically relates to the use of L-histidine in the preparation of products that increase the number of embryo implantation sites in animals. This invention provides, on the one hand, the use of L-histidine in the preparation of products that increase the number of embryo implantation sites in animals, and on the other hand, the use of a combination of L-histidine and a basal diet in the preparation of products that increase the number of embryo implantation sites in animals. This invention has demonstrated through rat experiments that, compared to a control group of rats fed a basal diet supplemented with a small amount of L-histidine, the experimental group of pregnant rats fed a basal diet supplemented with additional L-histidine showed a significantly increased number of embryo implantation sites.

Description

Technical Field

[0001] This invention belongs to the field of animal nutrition technology, and specifically relates to the use of L-histidine in the preparation of products that increase the number of implantation sites in animal embryos. Background Technology

[0002] Sows are the core of the pig industry, and their reproductive performance is a crucial factor determining the overall production level and economic benefits of pig farming. Embryo loss during gestation is a significant factor affecting sow reproductive performance. Approximately 30% to 50% of gestation losses occur in early gestation, and 75% of these losses are due to embryo implantation failure. Therefore, finding suitable ways to increase the number of early embryo implantations is of great importance for improving sow reproductive performance.

[0003] Existing technologies attempt to promote embryo implantation and increase the number of implantation sites by adding specific components to animal feed. For example, invention patent 202210849502.6 describes a composition, feed additive, and its preparation method and application for promoting sow embryo implantation. This composition includes a traditional Chinese medicine monomer and a traditional Chinese medicine; the monomer includes astragalus polysaccharide, astragaloside A, angelica polysaccharide, ferulic acid, total saponins of American ginseng, 6-gingerol, and dipsacusin IV; the traditional Chinese medicine includes cardamom and nutmeg. While this invention can promote embryo implantation and increase the number of implantation sites, it contains multiple components, making the combination of these components laborious, time-consuming, and expensive. Existing technologies also attempt to increase the number of implantation sites by adding a single component to feed, but the provided single components are expensive and lack economic viability. Summary of the Invention

[0004] This invention addresses the problems of existing technologies that require multiple components to be added to increase the number of implantation sites in animal embryos, which are laborious, time-consuming, and expensive, and that adding a single component is expensive and not economically viable. It provides the use of L-histidine in the preparation of products that increase the number of implantation sites in animal embryos, which is labor-saving, time-saving, and cost-effective, and can solve the above problems by using a single low-cost component.

[0005] The technical solution protected by this invention is as follows:

[0006] Uses of L-histidine in the preparation of products that increase the number of implantation sites in animal embryos.

[0007] The animal in question is a rat.

[0008] The products are selected from: feed, feed additives, and veterinary drugs.

[0009] Preferably, the content of L-histidine in the product is 0.4%-1.2% by mass.

[0010] Preferably, the content of L-histidine in the product is 0.8% or 1.2% by mass.

[0011] The use of a combination of L-histidine and basal feed in the preparation of products that increase the number of animal embryo implantation sites.

[0012] Preferably, the content of L-histidine in the product is 0.4%-1.2% by mass;

[0013] Preferably, the content of L-histidine in the product is 0.8% or 1.2% by mass.

[0014] The feed is selected from breeding feed.

[0015] Preferably, the feed is selected from the growth and reproduction feed for rats and mice.

[0016] The animal in question is a rat.

[0017] The products are selected from: feed, feed additives or veterinary drugs.

[0018] The beneficial effects of this invention are:

[0019] L-histidine is an important amino acid that plays a major role in protein synthesis, regulation of nervous system function, maintenance of cellular pH homeostasis, and health regulation (cardioprotection, improved insulin sensitivity, antioxidation, etc.). However, there are no reports in the field regarding L-histidine increasing the number of implantation sites in animal embryos. This invention provides the use of L-histidine in the preparation of products that increase the number of implantation sites in animal embryos, and provides the use of a combination of L-histidine and a basal diet in the preparation of such products. This invention finds that increasing the L-histidine content in a basal diet can significantly increase the number of implantation sites in rat embryos and promote embryo implantation in early pregnancy. This invention, verified through rat experiments, shows that compared to the control group containing only 0.4% L-His, the experimental groups with additional 0.4% and 0.8% L-His significantly increased the number of rat embryo implantation sites. This solves the problem of laborious, time-consuming, and expensive component combinations when using existing technologies to increase the number of animal embryo implantation sites. Furthermore, L-histidine is inexpensive and economical, solving the problem of high cost and poor cost-effectiveness of adding a single component to increase the number of animal embryo implantation sites using existing technologies. Attached Figure Description

[0020] Figure 1 This study investigated the effect of dietary supplementation with L-histidine during early pregnancy on the number of embryonic implantation sites on day 7 of gestation in rats. n = 11, and data were statistically analyzed using a t-test. *P < 0.05.

[0021] CON represents control rats fed a standard diet supplemented with 0.4% L-histidine by weight;

[0022] 0.4% L-His represents rats in the experimental group fed a standard diet supplemented with 0.8% L-histidine by weight;

[0023] 0.8% L-His represents rats in the experimental group fed a standard diet supplemented with 1.2% L-histidine by weight. Detailed Implementation

[0024] To further illustrate the present invention, the technical solutions provided by the present invention will be described in detail below with reference to the embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0025] Unless otherwise specified, all components described in this invention are readily available to those skilled in the art.

[0026] Sources of biomaterials

[0027] The rats used in the experimental examples of this invention are commercially available.

[0028] Group 1 Examples

[0029] This set of examples provides the use of histidine in the preparation of products that increase the number of implantation sites in animal embryos.

[0030] Those skilled in the art, based on the teachings of this invention, can use L-histidine to increase the number of embryo implantations or implantation sites in any animal (e.g., rats, pigs, cattle, sheep, rabbits, horses, donkeys, etc.). Any act of using L-His to increase the number of embryo implantations or implantation sites in animals, and / or any act of manufacturing L-His into a product that increases the number of embryo implantations or implantation sites in animals, and / or any act of including L-His in product packaging labeled as intended to increase the number of embryo implantations or implantation sites in animals, and any act of selling, offering for sale, using, producing, or preparing a product containing L-His and having the effect of increasing the number of embryo implantations or implantation sites in animals, falls within the scope of protection of this invention.

[0031] In a specific embodiment, the animal is a mouse.

[0032] Those skilled in the art, based on the teachings of this invention, can use L-His to increase the number of embryo implantations or implantation sites in animals other than mice (e.g., pigs, cattle, sheep, rabbits, horses, donkeys, etc.). Any act of using L-His to increase the number of animal embryo implantations or implantation sites, and / or any act of manufacturing L-His into a product that increases the number of animal embryo implantations or implantation sites, and / or any act of including L-His in product packaging labeled as intended for increasing the number of animal embryo implantations or implantation sites, and any act of selling, offering for sale, using, producing, or preparing a product containing L-His and having the effect of increasing the number of animal embryo implantations or implantation sites, falls within the scope of protection of this invention.

[0033] In some embodiments of the present invention, the product is selected from: feed, feed additives, and veterinary drugs.

[0034] Group 2 Examples

[0035] This set of examples provides the use of a combination of L-histidine and a basal diet in the preparation of products that increase the number of animal embryo implantation sites.

[0036] Those skilled in the art, based on the teachings of this invention, can use the combination of L-His and a basal feed to increase the number of implantation sites or implantation sites in embryos of any animal (e.g., rats, pigs, cattle, sheep, rabbits, horses, donkeys, etc.). Any act of using L-His and a basal feed to increase the number of implantation sites or implantation sites in animals, and / or any act of making a product from L-His and a basal feed to increase the number of implantation sites or implantation sites in animals, and / or any act of placing L-His and a basal feed in product packaging labeled as intended to increase the number of implantation sites or implantation sites in animals, and any act of selling, offering for sale, using, producing, or preparing a product containing L-His and a basal feed that has the effect of increasing the number of implantation sites or implantation sites in animals, falls within the scope of protection of this invention.

[0037] In a specific embodiment of the present invention, the content of L-His in the product is 0.4%-1.2% by mass.

[0038] In a preferred embodiment of the present invention, the content of L-His in the product is 0.8% or 1.2% by mass.

[0039] In some embodiments of the present invention, the base feed is selected from breeding feed.

[0040] In other embodiments of the present invention, the breeding feed is selected from rat and mouse growth and breeding feed.

[0041] In a specific embodiment, the animal is a mouse.

[0042] Those skilled in the art, based on the teachings of this invention, can use the combination of L-His and a basal feed to increase the number of embryo implantations or implantation sites in animals other than mice (e.g., pigs, cattle, sheep, rabbits, horses, donkeys, etc.). Any act of using the combination of L-His and a basal feed to increase the number of animal embryo implantations or implantation sites, and / or any act of making a product from L-His and a basal feed to increase the number of animal embryo implantations or implantation sites, and / or any act of placing L-His and a basal feed in product packaging labeled as intended for increasing the number of animal embryo implantations or implantation sites, and any act of selling, offering for sale, using, producing, or preparing a product containing L-His and a basal feed that has the effect of increasing the number of animal embryo implantations or implantation sites, falls within the scope of protection of this invention.

[0043] In other embodiments, the product is selected from feed, feed additives, or veterinary drugs.

[0044] Experimental Examples: Verification of the New Application of L-His in this Invention

[0045] 1. Test Methods

[0046] This study investigated the effect of dietary L-His supplementation on rat embryo implantation. Eight-week-old female Sprague Dawley rats (SD rats, purchased from Beijing Huafukang Biotechnology Co., Ltd.) were selected and housed under standard conditions (controlled temperature and humidity, 12 h light: 12 h darkness, free access to food and water). Males and females were mated together, and the presence of sperm in vaginal smears was defined as day 1 of gestation. Pregnant rats were fed standard rat / macula reproductive feed (control group, 0.4% L-His, CON group) or rat / macula reproductive feed supplemented with 0.4% (0.8% L-His) or 0.8% (1.2% L-His) (n = 11 per group). On day 7 of gestation, all rats were anesthetized by intravenous injection of 2.0% sodium pentobarbital (30 mg / kg BW), and the abdominal cavity was opened along the midline to expose the uterine horns. The number of implantation sites was recorded. The rat breeding feed was a commercially available rat and mouse breeding feed, and its nutritional value should meet the nutritional standards required for rat reproduction (AIN-93). It was purchased from Beijing Huafukang Biotechnology Co., Ltd.

[0047] 2. Experimental Results

[0048] L-His increased the implantation rate of rat embryos.

[0049] To determine the application of L-His in embryo implantation, the number of implanted embryos was measured on day 7 of gestation in rats in this experiment. Figure 1As shown, compared with the control group (CON), the addition of 0.4% L-His to the diet during early pregnancy tended to increase the number of embryo implantation sites (P = 0.05), and the addition of 0.8% L-His significantly increased the number of embryo implantation sites (P < 0.05). These results indicate that in early pregnancy, dietary supplementation with L-His can effectively increase the number of implantation sites in rat embryos and has a positive impact on rat embryo implantation.

[0050] Although the present invention has been disclosed above with reference to preferred embodiments, it is not intended to limit the present invention. Anyone skilled in the art can make various modifications and alterations without departing from the spirit and scope of the present invention. Therefore, the scope of protection of the present invention should be determined by the claims.

Claims

1. The use of L-histidine in the preparation of products that increase the number of implantation sites in animal embryos, characterized in that, The L-histidine content in the product is 0.8% or 1.2% by mass; the animal is a mouse; the product is selected from: feed, feed additives, and veterinary drugs.

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