Composition of nutritional additive and traditional Chinese medicine for removing pet tear marks and preparation method thereof

Abstract

The present invention provides a composition of a nutritional additive and a Chinese medicine for removing tear marks of pets and a preparation method thereof, wherein the composition comprises the following components: a nutritional additive and a compound Chinese medicine extract; the nutritional additive is composed of the following raw materials: vitamin A, vitamin E, taurine, and arginine; and the compound Chinese medicine extract is prepared from the following raw materials: prepared rehmannia, yam, tuckahoe, orientalis, angelica, ligusticum chuanxiong, wolfberry, chrysanthemum, and cassia bark. The composition of the present invention can effectively remove tear marks of pets, has a low recurrence rate, and has good long-term effectiveness; at the same time, the composition does not need to be taken for a long time, and has little side effects on pets.

Description

Technical Field

[0001] The invention relates to the field of removing pet tear marks, in particular to a composition of a nutritional additive and a traditional Chinese medicine for removing pet tear marks and a preparation method thereof. Background Art

[0002] The lacrimal gland is an accessory organ of the eye, and its main function is to secrete tears to moisten the eyeball. Under normal circumstances, the lacrimal gland secretes tears to moisten the eyeball and then enters the nasal cavity through the lacrimal duct, and will not flow out from the corner of the eye; however, if the lacrimal gland secretes too many tears or cannot be discharged from the nasolacrimal duct, tears will flow out from the corner of the eye. Excessive tears react with microorganisms attached to the hair around the eyes of pets (such as dogs, cats, etc.), forming brown, brown or pink tear marks, which may be accompanied by bad odor, moist dermatitis, skin ulcers, etc. in severe cases. Tear marks, also known as epiphora, not only affect the facial beauty of pets (such as dogs, cats, etc.), but continuous tearing will also cause the area around the pet's eyes to become a breeding ground for a series of bacteria, fungi, and fleas, causing eye infections and inflammation, causing physical pain to the pet.

[0003] The inventors have found that the causes of tear stains mainly include the following: eye infection, lacrimal gland inflammation, conjunctivitis, nasolacrimal duct obstruction, entropion, heredity, ear canal infection, excessive internal heat, dusty breeding environment, high salt content in feed, etc. It can be divided into the following aspects:

[0004] A. Dietary factors: Foods containing allergic substances or certain minerals lead to increased tear secretion, thus causing the tear marks to worsen.

[0005] B. Genetic factors: Some breeds of pets, especially dogs and cats with short noses, have large curvature of their nasolacrimal ducts. Even normal tears cannot be discharged through the nasolacrimal ducts, and flow out from the corners of the eyes, forming tear marks or even black eye sockets.

[0006] C. Pathological factors: (1) Ear infection. When the pet's ear canal is infected by bacteria, viruses, mold, or parasites, or the ear is swollen, painful, or itchy, the pet will frequently scratch its ear with its hind legs. (2) Nasal tear duct obstruction. When the nasolacrimal duct is infected and becomes inflamed and swollen, the entire tear duct is completely or partially blocked, and tears cannot be discharged from the nasolacrimal duct normally, thus forming unsightly tear marks. (3) Eye inflammation. When the tear glands, conjunctiva, or cornea of ​​the pet's eyes are infected by viruses or bacteria, it often causes abnormal and excessive tear secretion. The nasolacrimal duct is unable to drain the excess tears in time, thus causing tears to flow out.

[0007] D. Non-pathological factors: If the pet's eyelids are turned inward, the eyelashes will constantly irritate the cornea, which can also cause very serious tear stains. If the hair around the pet's eyes is too long, it will always poke into the eyes, constantly irritating the cornea or conjunctiva, which can also cause abnormal tear flow and form tear stains.

[0008] At present, the common methods for removing tear stains are mainly drug treatment and surgical treatment, but the recurrence rate is very high for both drug treatment and surgical treatment. The mechanism of action of systemic antibiotics and some newly developed tear stain removal drugs at home and abroad is similar. They all use drugs to control the content of microorganisms in tears to reduce the color of tear stains. If there is no tearing or the tearing is not serious, then this type of drug product can indeed achieve a good tear stain removal effect during the application process, but once the drug is stopped, the recurrence rate exceeds 90%, and the long-term effect of the drug is poor. At the same time, antibiotics and existing tear stain removal drugs have great side effects on pets. Long-term use will increase the burden on the liver and kidneys, greatly increase the risk of pets suffering from liver and kidney diseases, and have a great impact on the health and life span of pets. Summary of the invention

[0009] In order to solve the technical problems existing in the prior art, the present invention provides a composition of a nutritional additive and a traditional Chinese medicine for removing tear marks of pets and a preparation method, which can effectively remove tear marks of pets, has a low recurrence rate and good long-term effect; at the same time, the composition does not need to be taken for a long time and has little side effects on pets.

[0010] In order to solve the above technical problems, the technical solution adopted by the present invention is as follows:

[0011] The composition of nutritional additives and traditional Chinese medicine for removing pet tear stains includes the following components: nutritional additives, compound traditional Chinese medicine extracts;

[0012] Nutritional additives, composed of the following raw materials: vitamin A, vitamin E, taurine, arginine;

[0013] The compound Chinese medicine extract is made from the following raw materials: cooked rehmannia, yam, tuckahoe, oriental water plantain, angelica, ligusticum chuanxiong, wolfberry, chrysanthemum and cassia bark.

[0014] Preferably, the weight ratio of the nutritional additive to the composite Chinese herbal medicine extract is 1:1-1.2.

[0015] Furthermore, the nutritional additive is composed of the following raw materials: vitamin A 60000IU-100000IU, vitamin E 500-1000IU, taurine 10-15g, and arginine 10-20g.

[0016] Furthermore, the composite Chinese medicine extract is prepared from the following raw materials in parts by weight: 2-6 parts of cooked rehmannia, 1-4 parts of yam, 1-5 parts of poria, 1-4 parts of oriental alisma, 1-5 parts of angelica, 1-4 parts of ligusticum chuanxiong, 1-5 parts of wolfberry, 1-6 parts of chrysanthemum, and 2-6 parts of cassia bark.

[0017] The preparation method of the composition of the nutritional additive and Chinese medicine for removing pet tear stains comprises the following steps: preparing the composite Chinese medicine extract and the preparation;

[0018] The method for preparing the composite Chinese medicine extract comprises the following raw materials in parts by weight: 2-6 parts of cooked rehmannia, 1-4 parts of yam, 1-5 parts of poria, 1-4 parts of oriental rhizome, 1-5 parts of angelica, 1-4 parts of ligusticum chuanxiong, 1-5 parts of wolfberry, 1-6 parts of chrysanthemum, and 2-6 parts of cassia bark, extracting 2-3 times by water extraction, and combining the extracts; the extracts are concentrated, dried, and granulated to obtain the composite Chinese medicine extract;

[0019] The preparation method comprises mixing the composite Chinese herbal extract and the nutritional additive at a weight ratio of 1-1.2:1 to obtain a composition of the nutritional additive and the Chinese herbal medicine for removing tear stains of pets;

[0020] The nutritional additive is composed of the following raw materials: 60000IU-100000IU of vitamin A, 500-1000IU of vitamin E, 10-15g of taurine, and 10-20g of arginine.

[0021] Preferably, the preparation of the composite Chinese medicine extract consists of the following steps: preparing a first decoction, preparing a second decoction, preparing a third decoction, and post-processing;

[0022] The method for preparing the first decoction liquid comprises weighing each raw material by weight, mixing them evenly, soaking them in water, decocting them, and filtering them to obtain a filter residue and the first decoction liquid;

[0023] The method for preparing the second decoction liquid is to add water to soak the filter residue, decoct, and filter to obtain the second decoction liquid;

[0024] The method for preparing the third decoction is to combine the first decoction and the second decoction, and filter to obtain the third decoction;

[0025] The post-treatment method is as follows: the third decoction is concentrated by heating, filtered to remove solid impurities, mixed with maltodextrin and stevioside, dried, and granulated to obtain the composite Chinese medicine extract.

[0026] Preferably, in the preparation of the first decoction, the amount of water added is 12-16 times the total weight of the raw materials, the soaking time is 0.2-1h, and the decoction time is 0.2-1h.

[0027] Preferably, in the preparation of the second decoction, the amount of water added is 10-14 times the total weight of the raw materials, the soaking time is 0.2-1h, and the decoction time is 0.2-1h.

[0028] Preferably, in the post-treatment, the heating and concentrating temperature is 60-65°C, and the heating and concentrating process is performed until the relative density of the concentrated solution is 1.09-1.11 g / cm 3。

[0029] Preferably, in the post-treatment, the dry paste rate after concentration is 25.2-30.8%.

[0030] Preferably, in the post-treatment, the drying is spray drying, and after spray drying, the mixture is sieved through a 80-120 mesh screen and then granulated.

[0031] Compared with the prior art, the present invention has the following beneficial effects:

[0032] (1) The composition of the nutritional additive and Chinese medicine for removing tear marks of pets of the present invention maintains the integrity of cell membranes and epithelial tissues through the nutritional additives of specific composition, maintains the health of the conjunctival epithelium, corneal epithelium and nasolacrimal duct epithelium, prevents damage to these epithelial tissues, avoids inflammation and blockage; protects membrane tissues, prevents the production of peroxides, and prevents oxidation of tears to color tear marks; and enhances the repair ability of the cornea and promotes the formation of photoreceptor cells; and protects related tissues from damage caused by free radicals, improves the immunity of epithelial cells, and resists environmental invasion of epithelial cells; and regulates protein metabolism, promotes collagen synthesis, and is beneficial to the repair and health of mucosal injuries;

[0033] Through the specific composition of the compound Chinese herbal extract, for the liver blood deficiency; Qi and blood deficiency, or liver and kidney deficiency; evil toxin invasion, targeted prescription. The specific explanation of the prescription is: cooked rehmannia is sweet and moisturizing, enters the liver and kidney meridians, and is good at nourishing yin blood; Chinese yam can nourish the spleen and kidney, nourishing kidney yin and spleen to help the source of postnatal metabolism; any method of nourishing kidney essence must drain its "turbidity" in order to preserve its "clearness" and replenish yin essence, so adding Alisma can not only drain dampness to drain turbidity, but also prevent cooked rehmannia from being greasy; Poria strengthens the spleen and eliminates dampness, tastes sweet and is neutral in nature, and is used together with Chinese yam to nourish the spleen to build up circulation; Chinese angelica nourishes Blood circulation, Chuanxiong is pungent, dispersing, warming and unblocking, activating blood circulation and promoting qi, the combination of Rehmannia glutinosa, Angelica sinensis and Chuanxiong nourishes blood and replenishes the middle, so that blood replenishment does not stagnate blood and blood circulation does not cause bleeding; Lycium barbarum is sweet and mild in nature, nourishes the liver and kidney, and improves the eyesight; Chrysanthemum suppresses liver yang, clears the liver and improves eyesight, and Semen Cassiae cools the liver, calms the liver and improves eyesight. The combination of Lycium barbarum, Chrysanthemum and Semen Cassiae enhances the effect of improving the eyesight, and the combination of these medicines replenishes qi and blood, nourishes the liver and kidney and improves eyesight;

[0034] By combining a specific composition of nutritional additives with a specific formula of compound Chinese herbal extracts, pet tear stains can be effectively removed with a low recurrence rate and good long-term effectiveness; at the same time, the composition does not require long-term ingestion and has little side effects on pets.

[0035] (2) The composition of the nutritional additive and traditional Chinese medicine for removing tear stains of pets of the present invention has good long-term effect, is better than antibiotic treatment, and is less likely to relapse. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] Figure 1 This is a schematic diagram of tear stain classification of a test example of the present invention;

[0037] Figure 2 This is the result analysis diagram of the test case where the tear marks are level 0 (no abnormality);

[0038] Figure 3 This is the result analysis diagram of the tear mark level 1 in the test example;

[0039] Figure 4 This is the result analysis diagram of the tear mark level 2 in the test example;

[0040] Figure 5 This is the result analysis diagram of the tear mark level 3 in the test example;

[0041] Figure 6 This is the result analysis diagram of the tear mark level 4 in the test example;

[0042] Figure 7 This is the result analysis diagram of the tear mark level 5 in the test example;

[0043] Figure 2-7 In the table, within the same time range (such as 7d, 14d, 21d, 28d), the left column is the result of the antibiotic control group, and the right column is the result of the combination group; the lowercase letters marked on the top of the two columns are the same, indicating that there is no significant difference between the different treatment groups (p>0.05); the lowercase letters marked on the top of the two columns are different, indicating that there is a significant difference between the different treatment groups (p<0.05). DETAILED DESCRIPTION

[0044] In order to have a clearer understanding of the technical features, purposes and effects of the present invention, the specific implementation methods of the present invention are now described.

[0045] Example 1

[0046] This embodiment provides a composition of a nutritional additive and a traditional Chinese medicine for removing tear stains of pets, comprising the following components: a nutritional additive and a composite traditional Chinese medicine extract.

[0047] Among them, the nutritional additives are composed of the following raw materials: vitamin A 60000IUIU, vitamin E 500IU, taurine 10g, and arginine 10g.

[0048] The composite Chinese medicine extract is prepared from the following raw materials in parts by weight: 2 parts of cooked rehmannia, 1 part of yam, 1 part of tuckahoe, 1 part of oriental rhizome, 1 part of angelica, 1 part of ligusticum chuanxiong, 1 part of wolfberry, 1 part of chrysanthemum and 2 parts of cassia bark.

[0049] This embodiment also provides a method for preparing a composition of a nutritional additive and a traditional Chinese medicine for removing pet tear stains, specifically:

[0050] 1. Preparation of compound Chinese herbal medicine extracts

[0051] ①Preparation of the first decoction

[0052] Weigh the components of the aforementioned composite Chinese medicine, mix them evenly, put them in a decoction pot, soak them in water, decoct them, and filter them to obtain a first decoction liquid;

[0053] Among them, the amount of water added is 12 times the total weight of the components of the composite Chinese medicine, the soaking time is 0.2h, and the decoction time is 0.2h.

[0054] ②Preparation of the second decoction

[0055] Continue to add water to the decoction pot and boil, and filter to obtain the second decoction;

[0056] The amount of water added is 10 times the total weight of the components of the composite Chinese medicine, and the decoction time is 0.2h.

[0057] ③Preparation of the third decoction

[0058] The first decoction and the second decoction are combined and filtered to obtain a third decoction;

[0059] ④ Post-processing

[0060] The third decoction is heated and concentrated, filtered, and then maltodextrin and stevioside are added, dried, and granulated to obtain a composite Chinese medicine extract.

[0061] The heating concentration temperature is 60°C, and the relative density of the concentrated liquid is 1.09 g / cm 3 ; The dry cream rate after concentration is 25.2%.

[0062] Maltodextrin is added in an amount of 10% by mass of the concentrate.

[0063] The amount of steviol glycoside added is 0.005% of the concentrate mass.

[0064] In this embodiment, the drying method is spray drying, and after spray drying, the mixture is sieved through an 80-mesh sieve and then granulated.

[0065] 2. Preparation

[0066] The compound Chinese herbal medicine extract and the nutritional additive are mixed at a weight ratio of 1.1:1 to prepare a composition of the nutritional additive and Chinese herbal medicine for removing pet tear stains.

[0067] Example 2

[0068] This embodiment provides a composition of a nutritional additive and a traditional Chinese medicine for removing tear stains of pets, comprising the following components: a nutritional additive and a composite traditional Chinese medicine extract.

[0069] Among them, the nutritional additives are composed of the following raw materials: vitamin A 80000IU, vitamin E 750IU, taurine 12g, and arginine 15g.

[0070] The composite Chinese medicine extract is prepared from the following raw materials in parts by weight: 4 parts of cooked rehmannia, 3 parts of yam, 2.5 parts of tuckahoe, 2 parts of oriental alisma, 3 parts of angelica, 2 parts of ligusticum chuanxiong, 2.5 parts of wolfberry, 4 parts of chrysanthemum and 3.5 parts of cassia bark.

[0071] This embodiment also provides a method for preparing a composition of a nutritional additive and a traditional Chinese medicine for removing pet tear stains, specifically:

[0072] 1. Preparation of compound Chinese herbal medicine extracts

[0073] ①Preparation of the first decoction

[0074] Weigh the components of the aforementioned composite Chinese medicine, mix them evenly, put them in a decoction pot, soak them in water, decoct them, and filter them to obtain a first decoction liquid;

[0075] Among them, the amount of water added is 14 times the total weight of the components of the composite Chinese medicine, the soaking time is 0.5h, and the decoction time is 0.7h.

[0076] ②Preparation of the second decoction

[0077] Continue to add water to the decoction pot and boil, and filter to obtain the second decoction;

[0078] The amount of water added is 12 times the total weight of the components of the composite Chinese medicine, and the decoction time is 0.7h.

[0079] ③Preparation of the third decoction

[0080] The first decoction and the second decoction are combined and filtered to obtain a third decoction;

[0081] ④ Post-processing

[0082] The third decoction is heated and concentrated, filtered, and then maltodextrin and stevioside are added, dried, and granulated to obtain a composite Chinese medicine extract.

[0083] The heating concentration temperature is 61°C, and the relative density of the concentrated liquid is 1.10 g / cm 3 ; The dry paste rate after concentration is 28.7%.

[0084] Maltodextrin is added in an amount of 12% of the concentrate mass.

[0085] The amount of steviol glycoside added is 0.006% of the concentrate mass.

[0086] In this embodiment, the drying method is spray drying, and after spray drying, the mixture is sieved through a 100-mesh sieve and then granulated.

[0087] 2. Preparation

[0088] The compound Chinese herbal medicine extract and the nutritional additive are mixed at a weight ratio of 1:1 to prepare a composition of the nutritional additive and Chinese herbal medicine for removing pet tear stains.

[0089] Example 3

[0090] This embodiment provides a composition of a nutritional additive and a traditional Chinese medicine for removing tear stains of pets, comprising the following components: a nutritional additive and a composite traditional Chinese medicine extract.

[0091] Among them, the nutritional additives are composed of the following raw materials: vitamin A 100000IU, vitamin E 1000IU, taurine 15g, and arginine 20g.

[0092] The composite Chinese medicine extract is prepared from the following raw materials in parts by weight: 6 parts of cooked rehmannia, 4 parts of yam, 5 parts of tuckahoe, 4 parts of oriental alisma, 5 parts of angelica, 4 parts of ligusticum chuanxiong, 5 parts of wolfberry, 6 parts of chrysanthemum and 6 parts of cassia bark.

[0093] This embodiment also provides a method for preparing a composition of a nutritional additive and a traditional Chinese medicine for removing pet tear stains, specifically:

[0094] 1. Preparation of compound Chinese herbal medicine extracts

[0095] ①Preparation of the first decoction

[0096] Weigh the components of the aforementioned composite Chinese medicine, mix them evenly, put them in a decoction pot, soak them in water, decoct them, and filter them to obtain a first decoction liquid;

[0097] Among them, the amount of water added is 16 times the total weight of each component of the composite Chinese medicine, the soaking time is 1 hour, and the decoction time is 1 hour.

[0098] ②Preparation of the second decoction

[0099] Continue to add water to the decoction pot and boil, and filter to obtain the second decoction;

[0100] The amount of water added is 14 times the total weight of the components of the compound Chinese medicine, and the decoction time is 1 hour.

[0101] ③Preparation of the third decoction

[0102] The first decoction and the second decoction are combined and filtered to obtain a third decoction;

[0103] ④ Post-processing

[0104] The third decoction is heated and concentrated, filtered, and then maltodextrin and stevioside are added, dried, and granulated to obtain a composite Chinese medicine extract.

[0105] The heating concentration temperature is 65°C, and the relative density of the concentrated liquid is 1.11 g / cm 3 ; The dry paste rate after concentration is 30.8%.

[0106] Maltodextrin is added in an amount of 15% of the concentrate mass.

[0107] The amount of steviol glycoside added is 0.008% of the concentrate mass.

[0108] In this embodiment, the drying method is spray drying, and after spray drying, the mixture is sieved through a 120-mesh sieve and then granulated.

[0109] 2. Preparation

[0110] The compound Chinese herbal medicine extract and the nutritional additive are mixed at a weight ratio of 1.2:1 to prepare a composition of the nutritional additive and Chinese herbal medicine for removing pet tear stains.

[0111] Example 4

[0112] This embodiment provides a composition of a nutritional additive and a traditional Chinese medicine for removing tear stains of pets, comprising the following components: a nutritional additive and a composite traditional Chinese medicine extract.

[0113] Among them, the nutritional additives are composed of the following raw materials: vitamin A 80000IU, vitamin E 750IU, taurine 12g, and arginine 15g.

[0114] The composite Chinese medicine extract is prepared from the following raw materials in parts by weight: 4 parts of cooked rehmannia, 3 parts of yam, 2.5 parts of tuckahoe, 2 parts of oriental alisma, 3 parts of angelica, 2 parts of ligusticum chuanxiong, 2.5 parts of wolfberry, 4 parts of chrysanthemum and 3.5 parts of cassia bark.

[0115] This embodiment also provides a method for preparing a composition of a nutritional additive and a traditional Chinese medicine for removing pet tear stains, specifically:

[0116] 1. Preparation of compound Chinese herbal medicine extracts

[0117] ①Preparation of the first decoction

[0118] Weigh the components of the aforementioned composite Chinese medicine, mix them evenly, put them in a decoction pot, soak them in water, decoct them, and filter them to obtain a first decoction liquid;

[0119] Among them, the amount of water added is 14 times the total weight of the components of the composite Chinese medicine, the soaking time is 0.5h, and the decoction time is 0.7h.

[0120] ②Preparation of the second decoction

[0121] Continue to add water to the decoction pot and boil, and filter to obtain the second decoction;

[0122] The amount of water added is 12 times the total weight of the components of the composite Chinese medicine, and the decoction time is 0.7h.

[0123] ③Preparation of the third decoction

[0124] The first decoction and the second decoction are combined and filtered to obtain a third decoction;

[0125] ④ Post-processing

[0126] The third decoction is heated and concentrated, filtered, and then a synergist, konjac glucomannan, locust bean gum, and stevioside are added, dried, and granulated to obtain a composite Chinese medicine extract.

[0127] The heating concentration temperature is 61°C, and the relative density of the concentrated liquid is 1.10 g / cm 3 ; The dry paste rate after concentration is 28.7%.

[0128] The synergist is prepared by the following method:

[0129] A. Pretreatment

[0130] Calcium phosphate, hydroxyapatite and activated carbon are added into 5 times volume of pretreatment liquid, and after being evenly dispersed, acetic acid is added dropwise under stirring to adjust the pH to 5.5, the temperature is raised to 50°C, and after being kept warm and refluxed for 12 hours, the solid is filtered out, and the solid is washed with sufficient deionized water until it is neutral, and dried to obtain a pretreated material.

[0131] The pretreatment liquid is an ethanol solution in which γ-aminopropyltriethoxysilane and γ-glycidyloxypropyltrimethoxysilane are dissolved.

[0132] In the pretreatment solution, the concentration of γ-aminopropyltriethoxysilane is 5.2 wt %, the concentration of γ-glycidyloxypropyltrimethoxysilane is 2 wt %, and the volume concentration of the ethanol solution is 70%.

[0133] The weight ratio of calcium phosphate, hydroxyapatite and activated carbon is 2:5:0.8.

[0134] B. Composite treatment

[0135] The pretreated material was added into 30 times the weight of chitosan solution (concentration of 5wt%), and after being evenly dispersed, stirring was continued for 90 minutes; under stirring conditions, mulberry leaf extract, orange peel extract, and Pleurotus eryngii extract were added, and stirring was continued for 60 minutes; then, 0.4 times the volume of polyvinyl alcohol solution (concentration of 16wt%) was added at a feeding rate of 4mL / min, the temperature was controlled at 30°C, and stirring was carried out at 250rpm for 3.5h; 2.5 times the volume of glutaraldehyde solution (concentration of 45wt%) was continued to be added, the pH was adjusted to 5, stirring was continued for 2h, and freeze-drying was carried out to obtain a synergist.

[0136] Among them, the weight ratio of the pretreated material, mulberry leaf extract, orange peel extract and Pleurotus eryngii extract is 100:8:10:4.

[0137] Mulberry leaf extract, orange peel extract, and Pleurotus eryngii extract were all purchased from commercial sources, with mesh specifications of 100 mesh and extraction ratios of 10:1.

[0138] The amount of synergist added is 3.5% of the concentrate mass.

[0139] The amount of konjac glucomannan added was 0.9% of the concentrate mass.

[0140] The amount of locust bean gum added was 0.2 wt% of the concentrate.

[0141] The amount of steviol glycoside added is 0.006% of the concentrate mass.

[0142] In this embodiment, the drying method is freeze drying, and after freeze drying, the mixture is crushed and ground evenly, and granulated after passing through a 100-mesh sieve.

[0143] 2. Preparation

[0144] The compound Chinese herbal medicine extract and the nutritional additive are mixed at a weight ratio of 1.2:1 to prepare a composition of the nutritional additive and Chinese herbal medicine for removing pet tear stains.

[0145] Test Example 1

[0146] In 2022, 120 small white poodles who came to the Animal Hospital of Shandong Animal Husbandry and Veterinary Vocational College for treatment of tear stains were selected. At the same time, the small white poodles were clinically examined to have no inverted eyelashes, nasolacrimal duct hypoplasia or obstruction, and were fed in a standardized manner. The 120 small white poodles were divided into Group 1 and Group 2. Group 1 was treated with the composition of Example 2, and Group 2 was treated with chloramphenicol (antibiotic control group); a course of treatment was 7 days, and after four courses of treatment, the treatment effects were compared.

[0147] The method of using the composition of Example 2 is to add 0.8 wt % of the composition to the dog food of a small white poodle per day for conventional feeding.

[0148] Since there is no relevant standard for grading tear stains at home and abroad, this test example grades the tear stains of small poodles according to their color (dark brown, dark brown, light brown, pink, light pink), properties (dry, moist), and shape, which are divided into 5 levels:

[0149] Among them, level 1 means that only the hair near the inner corner of the eye changes color, the color is lighter, and the texture is dry or moist;

[0150] Level 2: The hair changes color linearly from the inner corner of the eye to the corner of the mouth, with a color change range of less than 1 cm, and the color is pink, light brown, dark brown or dark brown, and the texture is dry or moist;

[0151] Level 3: The linear discoloration range is greater than 1 cm, the color is darker, and the texture is moist;

[0152] Level 4: Not only is there linear discoloration, but also the hair on the lower eyelid from the inner corner to the outer corner of the eye changes color to a "crescent" shape, with a darker color and moist texture;

[0153] Level 5 means linear discoloration and a wider range of discoloration of the lower eyelid hair, which is like a "panda eye". Even the hair in the triangular area surrounded by the inner corner of the eye, the outer corner of the eye and the corner of the mouth changes color. The color is darker and moist, and may be accompanied by an unpleasant odor. See the attached manual for details. Figure 1 .

[0154] The therapeutic effects of the composition of Example 2 are shown in the following table:

[0155]

[0156] The treatment effects of the antibiotic control group are shown in the following table:

[0157]

[0158] Analyze the above effects:

[0159] Through comparative analysis of the preventive effect of pet tear stains at level 0 (no abnormality), such as Figure 2 As shown, starting from the 14th day, the number of normal animals in the composition group of Example 2 was significantly higher than that in the antibiotic control group (p<0.05), that is, the composition group of the present invention had a more significant preventive effect than the antibiotic control group.

[0160] Through comparative analysis of the first-level therapeutic effects on pet tear stains, the antibiotic control group had a better therapeutic effect in the first 14 days of treatment, and the therapeutic effect from the 7th day to the 14th day was significantly better than that of the combination group of Example 2 (p<0.05); but as time went on, the therapeutic effect of the combination group of Example 2 was equivalent to that of the antibiotic control group on the 21st day (p>0.05); by the 28th day, the combination group of Example 2 showed a more significant therapeutic effect (p<0.05).

[0161] Through comparative analysis of the secondary therapeutic effects on pet tear stains, from day 0 to day 21, there was no significant difference in the secondary therapeutic effect on pet tear stains between the antibiotic control group and the combination group of Example 2 (p>0.05), and on the 28th day, the appearance of the combination group of Example 2 decreased significantly (p<0.05).

[0162] Through comparative analysis of the 3-level therapeutic effects on pet tear stains, it was found that the therapeutic effect of the composition group of Example 2 was slightly worse than that of the antibiotic control group in the first 14 days; compared with the antibiotic control group in the same time period, the number of pet tear stains of 3 levels was relatively large, but from day 21 to day 28, the number of pet tear stains of 3 levels in the composition group of Example 2 was significantly decreased compared with that in the antibiotic control group (p<0.05).

[0163] Through comparative analysis of the 4-level therapeutic effects on pet tear stains, the number of 4-level tear stains of pets in the composition group of Example 2 in the first 14 days was higher than that in the antibiotic control group, and the therapeutic effect of the composition group of Example 2 was slightly worse than that of the antibiotic control group; however, from day 21 to day 28, the number of 3-level tear stains of pets in the composition group of Example 2 decreased significantly compared with the antibiotic control group (p<0.05), indicating that the composition of the present invention has a better therapeutic effect.

[0164] Through comparative analysis of the treatment effects on pet tear stains at level 5, the treatment effects of the antibiotic control group and the composition group of Example 2 on pet tear stains at level 5 increased with the increase in days, and there was no significant difference in the treatment effects between the two groups (p>0.05). On the 28th day, the pet tear stains at level 5 in the composition group of Example 2 were completely cured, and the effect was significantly better than that in the antibiotic control group.

[0165] Furthermore, a follow-up investigation was conducted on small white poodles treated with the composition of Example 2 and the antibiotic control group. One month after the end of the treatment with the composition of Example 2, there was no recurrence of tear marks, and the composition had good long-term effectiveness; while after the end of the antibiotic treatment, tear marks recurred one week after the drug was stopped.

[0166] It can be seen that the use of antibiotics for tear stain treatment has better immediacy, which is specifically manifested in that the effect is obvious in the first course of treatment, but the therapeutic effect of subsequent courses is significantly lower than that of the composition of the present invention; at the same time, compared with antibiotics, the composition of the present invention has good long-term effect and low recurrence rate, and the composition does not need to be taken for a long time, and has little side effects on pets.

[0167] Test Example 2

[0168] The same experimental conditions as those in Experimental Example 1 were used to select 60 small white poodles with the same conditions as those in Group 1-2. At the same time, each small white poodle was clinically examined to have no inverted eyelashes, nasolacrimal duct hypoplasia or obstruction, and was fed in a standardized manner. The 60 small white poodles were used as Group 3, and Group 3 was treated with the composition of Example 4; one course of treatment was 7 days, and after four courses of treatment, the treatment effects were compared.

[0169] The composition of Example 4 is used in the following manner: adding the composition to the dog food of a small white poodle at an addition amount of 0.6 wt % per day for conventional feeding.

[0170] The therapeutic effects of the composition of Example 4 are shown in the following table:

[0171]

[0172] It can be seen that the therapeutic effect of the pet tear stains using the composition of Example 4 is further improved on the basis of the therapeutic effect of the composition of Example 2. At the same time, after a follow-up investigation, there was no recurrence of tear stains four months after the end of the treatment with the composition of Example 4, and the composition had good long-term effectiveness. Further, after testing, the composition of Example 4 was stored at room temperature without being shielded from light for 6 months, and the therapeutic effect on pet tear stains was not significantly different from that of the newly prepared composition. The composition of Example 4 further improves the performance of removing pet tear stains, maintains a low recurrence rate, and has excellent long-term effectiveness, while further improving the storage stability of the composition.

[0173] Unless otherwise specified, all percentages used in the present invention are by mass.

[0174] Finally, it should be noted that the above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A composition of a nutritional additive and a Chinese medicine for removing tear stains of pets, characterized in that: It includes the following ingredients: nutritional additives, compound Chinese herbal medicine extracts; The nutritional additive is composed of the following raw materials: vitamin A 60000IU-100000IU, vitamin E 500-1000IU, taurine 10-15g, arginine 10-20g; The composite Chinese medicine extract is prepared from the following raw materials in parts by weight: 2-6 parts of cooked rehmannia, 1-4 parts of yam, 1-5 parts of tuckahoe, 1-4 parts of oriental rhizome, 1-5 parts of angelica, 1-4 parts of ligusticum chuanxiong, 1-5 parts of wolfberry, 1-6 parts of chrysanthemum, and 2-6 parts of cassia bark; The weight ratio of the nutritional additive to the composite Chinese medicine extract is 1:1-1.

2.

2. A method for preparing a composition of a nutritional additive and a traditional Chinese medicine for removing tear marks of pets, characterized in that: The method comprises the following steps: preparation and formulation of compound Chinese medicine extract; The method for preparing the composite Chinese medicine extract comprises the following raw materials in parts by weight: 2-6 parts of cooked rehmannia, 1-4 parts of yam, 1-5 parts of poria, 1-4 parts of oriental rhizome, 1-5 parts of angelica, 1-4 parts of ligusticum chuanxiong, 1-5 parts of wolfberry, 1-6 parts of chrysanthemum, and 2-6 parts of cassia bark, extracting 2-3 times by water extraction, and combining the extracts; the extracts are concentrated, dried, and granulated to obtain the composite Chinese medicine extract; The preparation method comprises mixing the composite Chinese herbal extract and the nutritional additive at a weight ratio of 1-1.2:1 to obtain a composition of the nutritional additive and the Chinese herbal medicine for removing tear stains of pets; The nutritional additive is composed of the following raw materials: 60000IU-100000IU of vitamin A, 500-1000IU of vitamin E, 10-15g of taurine, and 10-20g of arginine.

3. The method for preparing the composition of the nutritional additive and traditional Chinese medicine for removing tear marks of pets according to claim 2, characterized in that: The preparation of the composite Chinese medicine extract comprises the following steps: preparing a first decoction, preparing a second decoction, preparing a third decoction, and post-processing; The method for preparing the first decoction liquid comprises weighing each raw material by weight, mixing them evenly, soaking them in water, decocting them, and filtering them to obtain a filter residue and the first decoction liquid; The method for preparing the second decoction liquid is to add water to soak the filter residue, decoct, and filter to obtain the second decoction liquid; The method for preparing the third decoction is to combine the first decoction and the second decoction, and filter to obtain the third decoction; The post-treatment method is as follows: the third decoction is concentrated by heating, filtered to remove solid impurities, mixed with maltodextrin and stevioside, dried, and granulated to obtain the composite Chinese medicine extract.

4. The method for preparing the composition of the nutritional additive and traditional Chinese medicine for removing tear marks of pets according to claim 3, characterized in that: In the preparation of the first decoction, the amount of water added is 12-16 times the total weight of the raw materials, the soaking time is 0.2-1h, and the decoction time is 0.2-1h.

5. The method for preparing the composition of the nutritional additive and traditional Chinese medicine for removing pet tear marks according to claim 3, characterized in that: In the preparation of the second decoction, the amount of water added is 10-14 times the total weight of the raw materials, the soaking time is 0.2-1h, and the decoction time is 0.2-1h.

6. The method for preparing the composition of the nutritional additive and traditional Chinese medicine for removing pet tear marks according to claim 3, characterized in that: In the post-treatment, the heating and concentrating temperature is 60-65°C, and the relative density of the concentrated liquid is 1.09-1.11 g / cm 3 .

7. The method for preparing the composition of the nutritional additive and traditional Chinese medicine for removing pet tear marks according to claim 3, characterized in that: In the post-treatment, the dry paste rate after concentration is 25.2-30.8%.

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