A method for efficiently extracting corn husk feruloyl arabinoxylan

Abstract

The invention discloses a method for efficiently extracting feruloylarabinoxylan, comprising the following steps: pre-processing agricultural product processing byproducts through ball milling, then sieving and separating to obtain ball milled agricultural product processing byproducts; mixing the ball milled agricultural product processing byproducts with water, then performing microwave heating extraction; and filtering the obtained extract through a filter membrane after centrifugation to obtain a clarified feruloylarabinoxylan extract. The extraction method adopted by the invention has no intervention of exogenous chemical or biological reagents, has few steps, and has the advantages of being green, efficient, and highly operable. The extraction method of the invention has excellent effects. The corn husk is used as a raw material, pre-processed through ball milling, and then microwaved. The concentration of feruloylarabinoxylan in the obtained extract is significantly increased, and the molecular weight is only 16% of that of the non-ball milled group. Under preferred conditions, the content of feruloylarabinoxylan in the extract can reach 5.9 mmol / L, which is much higher than the existing technology level.

Description

Technical Field

[0001] The invention belongs to the technical field of high-value utilization of agricultural and sideline products, and particularly relates to a method for efficiently extracting corn husk feruloyl arabinoxylan. Background Art

[0002] Feruloylarabinoxylan refers to a type of soluble functional oligosaccharides in which part of the oligosaccharide side chain is replaced by ferulic acid. It is widely found in cereal bran and is usually obtained by bioenzymatic method, heat treatment or weak acid hydrolysis. Feruloylarabinoxylan has been reported to have stronger antioxidant properties than ferulic acid and can effectively inhibit the oxidation of human low-density lipoprotein. In addition, feruloylarabinoxylan has good anti-thrombotic, intestinal function prebiotic and immunostimulatory effects. And feruloylarabinoxylan is stable under low pH and high temperature conditions, which determines that it has a wide range of applications in the food, pharmaceutical and cosmetic industries.

[0003] my country's agricultural product processing byproduct resources are rich, and the annual production of wheat bran, corn bran, rice bran and other cereal brans exceeds 80 million tons. These byproducts are good natural sources of feruloyl arabinoxylan. The efficient extraction of feruloyl arabinoxylan is one of the effective ways to utilize agricultural and sideline products at a high value. However, the main component of these cereal brans is lignocellulose, which has a complex and intertwined anti-degradation structure. The extraction efficiency of feruloyl arabinoxylan using the prior art is low, and the substrate is not fully utilized. For example, patent application CN 1840673A uses a multi-step enzymatic hydrolysis method to prepare wheat bran feruloyl arabinoxylan, and its concentration is only 1.546mmol / L. Therefore, it is urgent to combine an effective extraction technology with a suitable pretreatment method to break the stubborn wood fiber structure and efficiently extract the target product.

[0004] Microwave extraction has good effects in extracting active compounds such as natural pigments, flavonoids, alkaloids, etc. It is a new type of extraction technology with low energy consumption, fast and green, and has good prospects. Ball milling technology breaks the plant cell structure through mechanical crushing, causing mechanical fracture of macromolecular substances. It is considered to be an effective solution to open the anti-degradation barrier of lignocellulose, and it has been proven that no adverse inhibitors are formed. The existing common preparation technology of feruloylarabinoxylan is dominated by enzyme method, supplemented by physical and chemical means to enhance the yield, but it still cannot achieve efficient extraction. There are no reports on the extraction of feruloylarabinoxylan by ball milling pretreatment and ball milling pretreatment combined with microwave extraction. Summary of the invention

[0005] In view of the above problems and / or the problems existing in the prior art, the present invention is proposed. The purpose of the present invention is to provide a method for efficiently extracting corn husk feruloyl arabinoxylan in view of the problems that the existing feruloyl arabinoxylan extraction efficiency is low and the process is complicated.

[0006] The present invention provides a method for efficiently extracting feruloyl arabinoxylan, which comprises the following steps:

[0007] Step 1) ball milling pretreatment: adding the agricultural product processing byproduct into the sample chamber of the ball mill, and adding grinding media, and sieving to separate the grinding media and the sample after ball milling, so as to obtain the ball milled agricultural product processing byproduct;

[0008] Step 2) extracting feruloylarabinoxylan: mixing the ball-milled agricultural product processing byproduct with water and then performing microwave heating extraction;

[0009] Step 3) Obtaining a feruloyl arabinoxylan extract: Pass the supernatant of the centrifuged extract after the heat treatment through a 0.1-0.80 μm filter membrane (preferably a 0.1-0.45 μm filter membrane, more preferably a 0.22 μm filter membrane) to obtain a clarified feruloyl arabinoxylan extract.

[0010] Specifically, the agricultural product processing by-products include one or more of corn brans, wheat bran, rice bran and other cereal brans.

[0011] Preferably, in the ball milling pretreatment, the ball milling medium is zirconia balls, the ball-to-material volume ratio is 2:1, and the effective ball milling time is 5-480 min, preferably 20-100 min, most preferably 50-70 min, and more specifically 60 min.

[0012] More preferably, the ball-milled corn husk is mixed with water, wherein the solid-liquid ratio is 1:50 to 1:2, preferably 1:20 to 1:4.

[0013] In a preferred embodiment, the microwave heating temperature is 60-200°C, preferably 110-140°C.

[0014] Further preferably, in the microwave heating, the heating time is 1-300 min, preferably, the heating time is 30-90 min, more preferably, the heating time is 60 min, and the temperature is naturally lowered to room temperature after the heating is completed.

[0015] More preferably, during the microwave heating, the initial pressure of the microwave heating system is not less than 4.5 bar.

[0016] In addition, preferably, the centrifugation condition in step 3) is 3000-10000 rpm for 5-30 min, preferably 5000 rpm for 10 min.

[0017] The invention provides a product containing feruloyl arabinoxylan obtained by the method for efficiently extracting feruloyl arabinoxylan.

[0018] Beneficial effects of the present invention:

[0019] (1) Ball milling pretreatment is a physical pretreatment method. No adverse by-products are formed. Ball milling destroys the physical structure of the material, increasing the contact area between the substrate and the extraction solvent. At the same time, the macromolecular substances are mechanically broken, which promotes the release of active ingredients in the material, improves the utilization efficiency of raw materials and the quality of products. After ball milling, the molecular weight of feruloyl arabinoxylan in the extract is reduced to 16% of that before ball milling, and the product quality is greatly improved.

[0020] (2) The present invention adopts microwave heating and uses water as the extraction solvent. No chemical or biological reagents are introduced in the whole process, and the extraction steps are few and simple, which conforms to the principles of green, healthy and sustainable development and has strong operability.

[0021] (3) The present invention combines microwave heating with ball milling pretreatment to form a synergistic effect. Compared with the conventional high-temperature extraction method of conventional coarse powder, the combination of ball milling and microwave can increase the content of feruloyl arabinoxylan in the extract by 7.85 times and the antioxidant property of the extract by 2.76 times. The content of feruloyl arabinoxylan in the extract can reach 5.9 mmol / L, which is much higher than the existing technical level. It is an efficient and advanced extraction method. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following briefly introduces the drawings required for describing the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative labor. Among them:

[0023] Figure 1 This is a graph showing the effects of ball milling pretreatment and heating methods (Examples 1 to 4) on the content of feruloylarabinoxylan in the extract.

[0024] Figure 2 This is a graph showing the effects of ball milling pretreatment and heating methods (Examples 1 to 4) on the antioxidant properties of the extract.

[0025] Figure 3 This is a graph showing the effect of different microwave temperatures (Example 1, Examples 5-8) on the extraction of feruloylarabinoxylan from ball-milled corn husk. DETAILED DESCRIPTION

[0026] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the specific implementation methods of the present invention are described in detail below in conjunction with the embodiments of the specification.

[0027] In the following description, many specific details are set forth to facilitate a full understanding of the present invention, but the present invention may also be implemented in other ways different from those described herein, and those skilled in the art may make similar generalizations without violating the connotation of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0028] Secondly, the term "one embodiment" or "embodiment" as used herein refers to a specific feature, structure, or characteristic that may be included in at least one implementation of the present invention. The term "in one embodiment" that appears in different places in this specification does not necessarily refer to the same embodiment, nor does it refer to a separate or selective embodiment that is mutually exclusive with other embodiments.

[0029] The feruloyl arabinoxylan assay of the present invention: the feruloyl arabinoxylan content is determined according to the molar concentration of bound ferulic acid in the extract. The method for determining the bound ferulic acid content is as follows: take 0.5 mL of the extract, add 2.5 mL of the sample and 3 mL of 1M NaOH, and place in a water bath at 100°C for 90 min. After cooling, adjust the pH to neutral with 1M HCl and then filter through a 0.22 μm filter membrane. The filtrate is measured by high performance liquid chromatography (with an ultraviolet detector) to determine the total ferulic acid content. The free ferulic acid content is measured by taking the extract diluted 12 times. The bound ferulic acid content is the difference between the total ferulic acid and the free ferulic acid content. The specific liquid phase parameters are as follows: injection volume 20 μL, mobile phase A: formic acid-water (0.05%) and mobile phase B: formic acid-acetonitrile (0.05%), flow rate 0.5 mL / min, and detection wavelength 320 nm.

[0030] The antioxidant determination method of the extract of the present invention is as follows: 3 mL of the extract diluted 36 times is added with 3 mL of DPPH solution, and the mixture is reacted in the dark for 30 minutes, and then the absorbance is determined at 517 nm. Ethanol is used to replace the sample to determine the DPPH absorbance. The absorbance determined by using ethanol instead of DPPH eliminates the sample background error, and the DPPH clearance rate (%) is calculated based on the change in the absorbance.

[0031] Materials used in the present invention: Corn husks were provided by Baolingbao Biological Co., Ltd.

[0032] Equipment used in the present invention: ball mill: CJM-SY-B; China Hebei Qinhuangdao Taijihuan Nano Co., Ltd.; microwave chemistry platform: MA167-002, Italy Milestone; high performance liquid chromatograph: Waters e2695, USA Waters; other raw materials and equipment are commercially available unless otherwise specified.

[0033] Embodiment 1:

[0034] The method for extracting feruloylarabinoxylan by ball milling and microwave assisted extraction of feruloylarabinoxylan in this embodiment comprises:

[0035] (1) Corn husk ball milling pretreatment: Corn husk and zirconia beads were mixed in a volume ratio of 1:2 and added into the sample chamber of a ball mill. After ball milling for 60 min, the sample and zirconia balls were separated by sieving to obtain ball-milled corn husk.

[0036] (2) Microwave extraction of feruloylarabinoxylan from ball-milled corn husk: Take 1 part of ball-milled corn husk and add 10 parts of water. Mix well and put it into a microwave device. Pass nitrogen gas to make the initial pressure exceed 4.5 bar, raise the temperature to 120°C, and heat for 60 min.

[0037] (3) Obtaining feruloylarabinoxylan extract: The cooled extract was centrifuged at 5000 rpm for 10 min and then filtered through a 0.22 μm filter membrane to obtain a clarified feruloylarabinoxylan extract.

[0038] Comparative Example 1:

[0039] The method for extracting feruloylarabinoxylan by microwave in this comparative example comprises:

[0040] (1) Microwave extraction of feruloylarabinoxylan: Take 1 part of coarse corn husk and add 10 parts of water, mix well and put into a microwave device, introduce nitrogen to make the initial pressure exceed 4.5 bar, set to 120°C, and heat for 60 min.

[0041] (2) Obtaining feruloylarabinoxylan extract: The cooled extract was centrifuged at 5000 rpm for 10 min and then filtered through a 0.22 μm filter membrane to obtain a clarified feruloylarabinoxylan extract.

[0042] Comparative Example 2:

[0043] The conventional heating extraction method of ball-milled corn husk feruloyl arabinoxylan in this comparative example comprises:

[0044] (1) Corn husk ball milling pretreatment: Corn husk and zirconia beads were mixed in a volume ratio of 1:2 and added into the sample chamber of a ball mill. After ball milling for 60 min, the sample and zirconia balls were separated by sieving to obtain ball-milled corn husk.

[0045] (2) Traditional heating extraction of feruloyl arabinoxylan from ball-milled corn husk: Take 1 part of ball-milled corn husk and add 10 parts of water. Mix well and put into the autoclave. Set the target temperature to 120°C and the heating time to 60 min.

[0046] (3) Obtaining feruloylarabinoxylan extract: The cooled extract was centrifuged at 5000 rpm for 10 min and then filtered through a 0.22 μm filter membrane to obtain a clarified feruloylarabinoxylan extract.

[0047] Comparative Example 3:

[0048] The conventional heating method for extracting corn husk feruloyl arabinoxylan in this comparative example comprises:

[0049] (1) Traditional heating extraction of feruloyl arabinoxylan from corn husk: Take 1 part of coarse corn husk and add 10 parts of water, mix well and put into the autoclave, set the target temperature to 120℃ and the heating time to 60min.

[0050] (2) Obtaining feruloylarabinoxylan extract: The cooled extract was centrifuged at 5000 rpm for 10 min and then filtered through a 0.22 μm filter membrane to obtain a clarified feruloylarabinoxylan extract.

[0051] The feruloylarabinoxylan extracts obtained in Example 1 and Comparative Examples 2 to 4 were subjected to feruloylarabinoxylan content and antioxidant activity tests. Figure 1 and Figure 2 .

[0052] Depend on Figure 1 It can be seen that the content of feruloyl arabinoxylan in the extract of corn husk conventionally heated at 120°C is 0.65 mmol / L. After the corn husk is ball-milled and then subjected to conventional heating and water extraction, the content of feruloyl arabinoxylan increases by 2.03 times. After the extraction heating method is changed from conventional heating to microwave heating, the content of feruloyl arabinoxylan in the extract increases significantly. The content of feruloyl arabinoxylan in the microwave ball-milled corn husk extract is 3.87 times that of the conventional ball-milled corn husk extract and 7.85 times that of the conventional coarse corn husk extract. Feruloyl imparts antioxidant properties to oligosaccharides, and the DPPH scavenging rates of the extracts described in Example 1 and Comparative Examples 2 to 4 are measured to characterize their antioxidant properties. Figure 2 It can be seen that ball milling pretreatment can enhance the antioxidant activity of the extract. The DPPH removal rate increased by 1.15 times under the traditional heating extraction method and increased by 1.58 times under microwave extraction. In addition, microwave extraction is also an effective way to improve the antioxidant activity of the extract. Compared with traditional heating extraction, microwave energy can increase the antioxidant activity of coarse corn husk extract by 1.76 times and the antioxidant activity of ball milled corn husk by 2.42 times. The antioxidant enhancement effect of microwave technology on ball milled corn husk extract is more obvious. Therefore, the combination of ball milling pretreatment and microwave heating has a synergistic enhancement effect on the extraction of feruloyl arabinoxylan and the antioxidant activity of the extract, and is a preferred and efficient extraction method.

[0053] The molecular weight of the feruloylarabinoxylan extracts described in Example 1 and Comparative Example 1 was measured, and the results are shown in Table 1:

[0054] Table 1 Molecular weight of feruloylarabinoxylan under different extraction conditions

[0055] Extraction method Weight average molecular weight Number average molecular weight Comparative Example 1 (Microwave) 34505 24782 Example 1 (Ball Milling-Microwave) 5534 1628

[0056] Combining Table 1 and Figure 1 It can be seen that after ball milling of corn husk, not only the concentration of feruloyl arabinoxylan in the extract was greatly increased, but also the molecular weight of the obtained feruloyl arabinoxylan was only 16% of that of the unmilled group. It is well known that the beneficial functionality of oligosaccharides increases with the decrease of molecular weight, so ball milling-microwave extraction is a promising means to improve the yield and quality of corn husk feruloyl arabinoxylan.

[0057] Embodiment 2:

[0058] The method for extracting feruloylarabinoxylan by ball milling and microwave assisted extraction of feruloylarabinoxylan in this embodiment comprises:

[0059] (1) Corn husk ball milling pretreatment: Corn husk and zirconia beads were mixed in a volume ratio of 1:2 and added into the sample chamber of a ball mill. After ball milling for 60 min, the sample and zirconia balls were separated by sieving to obtain ball-milled corn husk.

[0060] (2) Microwave extraction of feruloylarabinoxylan from ball-milled corn husk: Take 1 part of ball-milled corn husk and add 10 parts of water. Mix well and put it into a microwave device. Pass nitrogen gas to make the initial pressure exceed 4.5 bar, raise the temperature to 100°C, and heat for 60 min.

[0061] (3) Obtaining feruloylarabinoxylan extract: The cooled extract was centrifuged at 5000 rpm for 10 min and then filtered through a 0.22 μm filter membrane to obtain a clarified feruloylarabinoxylan extract.

[0062] Embodiment 3:

[0063] The method for extracting feruloylarabinoxylan by ball milling and microwave assisted extraction of feruloylarabinoxylan in this embodiment comprises:

[0064] (1) Corn husk ball milling pretreatment: Corn husk and zirconia beads were mixed in a volume ratio of 1:2 and added into the sample chamber of a ball mill. After ball milling for 60 min, the sample and zirconia balls were separated by sieving to obtain ball-milled corn husk.

[0065] (2) Microwave extraction of feruloylarabinoxylan from ball-milled corn husk: Take 1 part of ball-milled corn husk and add 10 parts of water. Mix well and put it into a microwave device. Pass nitrogen gas to make the initial pressure exceed 4.5 bar, raise the temperature to 110°C, and heat for 60 min.

[0066] (3) Obtaining feruloylarabinoxylan extract: The cooled extract was centrifuged at 5000 rpm for 10 min and then filtered through a 0.22 μm filter membrane to obtain a clarified feruloylarabinoxylan extract.

[0067] Embodiment 4:

[0068] The method for extracting feruloylarabinoxylan by ball milling and microwave assisted extraction of feruloylarabinoxylan in this embodiment comprises:

[0069] (1) Corn husk ball milling pretreatment: Corn husk and zirconia beads were mixed in a volume ratio of 1:2 and added into the sample chamber of a ball mill. After ball milling for 60 min, the sample and zirconia balls were separated by sieving to obtain ball-milled corn husk.

[0070] (2) Microwave extraction of feruloylarabinoxylan from ball-milled corn husk: Take 1 part of ball-milled corn husk and add 10 parts of water. Mix well and put it into a microwave device. Pass nitrogen gas to make the initial pressure exceed 4.5 bar, raise the temperature to 130°C, and heat for 60 min.

[0071] (3) Obtaining feruloylarabinoxylan extract: The cooled extract was centrifuged at 5000 rpm for 10 min and then filtered through a 0.22 μm filter membrane to obtain a clarified feruloylarabinoxylan extract.

[0072] Embodiment 5:

[0073] The method for extracting feruloylarabinoxylan by ball milling and microwave assisted extraction of feruloylarabinoxylan in this embodiment comprises:

[0074] (1) Corn husk ball milling pretreatment: Corn husk and zirconia beads were mixed in a volume ratio of 1:2 and added into the sample chamber of a ball mill. After ball milling for 60 min, the sample and zirconia balls were separated by sieving to obtain ball-milled corn husk.

[0075] (2) Microwave extraction of feruloylarabinoxylan from ball-milled corn husk: Take 1 part of ball-milled corn husk and add 10 parts of water. Mix well and put it into a microwave device. Pass nitrogen gas to make the initial pressure exceed 4.5 bar, raise the temperature to 140°C, and heat for 60 min.

[0076] (3) Obtaining feruloylarabinoxylan extract: The cooled extract was centrifuged at 5000 rpm for 10 min and then filtered through a 0.22 μm filter membrane to obtain a clarified feruloylarabinoxylan extract.

[0077] Prior art example 1

[0078] The extraction technology of feruloyl arabinoxylan refers to the prior art 1 (Jie Chunyan, You Xuejiao, Hou Xiaoqiang, et al. Effect of physical treatment of corn husk residue on enzymatic extraction of feruloyl oligosaccharides [J]. Food Industry Science and Technology, 2016, 37(08)). The content of corn husk-derived feruloyl arabinoxylan extracted was 0.253 mmol / L.

[0079] Prior art example 2

[0080] The extraction technology of feruloyl arabinoxylan refers to the prior art 2 (Gao Xiaoli, Cheng Ziyue. Extraction of feruloyl oligosaccharides from millet bran and study on its antioxidant activity [J]. Grain and Oil, 2023, 36 (10)). The content of feruloyl arabinoxylan from millet bran obtained by extraction is 0.0045 mmol / L.

[0081] Prior art example 3

[0082] The extraction technology of feruloyl arabinoxylan refers to the prior art 2 (Lingxiao Gong, Danning Feng, Jie Liu, et al. Ionic liquid depolymerize the lignocellulose for the enzymatic extraction of feruloylated oligosaccharide from corn bran [J]. Food Chemistry: X, 2022, 15 100381). The content of feruloyl arabinoxylan from corn bran obtained by extraction is 0.906 mmol / L.

[0083] The feruloylarabinoxylan extracts obtained in Examples 1 to 5 were subjected to determination of feruloylarabinoxylan content ( Figure 3 ), and compared and analyzed with the existing technology:

[0084] Depend on Figure 3It can be seen that when the microwave temperature is between 100 and 130 ° C, the concentration of feruloyl arabinoxylan in the extract increases with the increase of temperature, reaching a maximum value at 130 ° C, and the content of feruloyl arabinoxylan decreases slightly when the temperature is further increased from 130 ° C to 140 ° C. When the extraction temperature is 130 ° C, the concentration of feruloyl arabinoxylan in the ball-milled corn husk extract is 5.9 mmol / L, and when the microwave temperature is 100 ° C, the concentration of feruloyl arabinoxylan obtained is the lowest, which is 1.681 mmol / L. The existing mainstream technology requires enzymatic method to obtain feruloyl arabinoxylan, but the enzyme is expensive and difficult to recover. And because the cell wall structure is complex, different components are intertwined and entangled, which makes it difficult for the enzyme to act accurately and efficiently on the corresponding glycosidic bond sites. Therefore, it is difficult to obtain feruloyl arabinoxylan by a single enzyme method, and other means are often needed to enhance the catalytic efficiency of the enzyme, including cellulase assistance (prior art 2), high pressure cooking treatment and cellulase assistance (prior art 1), ionic liquid pretreatment (prior art 3). The concentration of feruloyl arabinoxylan obtained after strengthening is 0.0045-0.906 mmol / L. Compared with these technologies, the present invention uses a ball milling-microwave method to avoid the use of high-priced enzymes, no exogenous chemical or biological reagents are involved, and the efficiency of extracting feruloyl arabinoxylan is significantly higher, with a concentration of 1.681-5.9 mmol / L, which has the advantages of being green, efficient and low-cost.

[0085] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the spirit and scope of the technical solutions of the present invention, which should all be included in the scope of the claims of the present invention.

Claims

1. A method for extracting feruloylarabinoxylan, characterized in that: The following steps are included: Step 1) Ball milling pretreatment: Add the agricultural product processing by-product into the sample chamber of the ball mill, and add grinding media. After ball milling, the grinding media and the sample are separated by sieving to obtain the ball milled agricultural product processing by-product; the ball milling medium is zirconia ball, the ball-to-material volume ratio is 2:1, and the effective ball milling time is 20-100 minutes; Step 2) Extracting feruloyl arabinoxylan: mixing the ball-milled agricultural product processing byproduct with water and then performing microwave heating extraction; the microwave heating temperature is 110-140°C; the heating time is 30-90 min, and the temperature is naturally lowered to room temperature after the heating is completed; the initial pressure of the microwave heating system is not less than 4.5 bar; the ball-milled agricultural product processing byproduct is mixed with water, wherein the solid-liquid ratio is 1:50-1:2; Step 3) obtaining a feruloyl arabinoxylan extract: filtering the supernatant of the heat-treated extract after centrifugation through a 0.1-0.80 µm filter membrane to obtain a clarified feruloyl arabinoxylan extract; the centrifugation condition is 3000-10000 rpm for 5-30 min; The agricultural product processing by-product is corn husk.

2. The method for extracting feruloyl arabinoxylan according to claim 1, characterized in that: In the step 1), the effective ball milling time is 50-70 minutes.

3. The method for extracting feruloyl arabinoxylan according to claim 1, characterized in that: In the step 1) of ball milling pretreatment, the effective ball milling time is 60 min.

4. The method for extracting feruloyl arabinoxylan according to claim 2, characterized in that: In the step 1), the ball-milled corn husk is mixed with water, wherein the solid-liquid ratio is 1:20-1:

4.

5. The method for extracting feruloyl arabinoxylan according to claim 1, characterized in that: In the step 2), the heating time is 60 min, and the temperature is naturally cooled to room temperature after the heating is completed.

6. The method for extracting feruloylarabinoxylan according to any one of claims 1 to 5, characterized in that: In step 3), the supernatant of the centrifuged extract after the heat treatment is filtered through a 0.1-0.45 µm filter membrane.

7. The method for extracting feruloylarabinoxylan according to any one of claims 1 to 5, characterized in that: The supernatant of the centrifuged extract was filtered through a 0.22 µm filter membrane.

8. The method for extracting feruloyl arabinoxylan according to claim 1, characterized in that: The centrifugal condition in step 3) is 4000-6000 rpm.

9. The method for extracting feruloyl arabinoxylan according to claim 1, characterized in that: The centrifugation condition in step 3) is 5000 rpm for 10 min.

10. A product containing feruloylarabinoxylan obtained by the method for extracting feruloylarabinoxylan according to any one of claims 1 to 9.

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