Scalp care anti-hair loss compositions and uses thereof, shampoo products and methods of making same

Abstract

The application relates to the field of cosmetics, in particular to a scalp care anti-hair loss composition and application thereof, a shampoo product and a preparation method thereof. The scalp care anti-hair loss composition comprises, in percentage by mass, ginger juice 1.0-35%, allantoin 0.01-2.0%, panthenol 0.1-10.0%, cypress leaf extract 0.1-5%, hydrolyzed chitin 0.005-0.1% and salicylic acid 0.01-0.5%, and the rest is water. The scalp care anti-hair loss composition provided by the application realizes synergistic effects through synergistic cooperation between the components; the components are maximally utilized, the scalp is nursed in multiple dimensions from multiple channels of the scalp hair follicle and scalp inflammation, the scalp is more nutritious, the scalp is healthier, the anti-hair loss effect is in the best ideal state, and finally the anti-hair loss effect is achieved.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of cosmetics, in particular, to a scalp care anti-hair loss composition and application thereof, a shampoo product and a preparation method thereof. BACKGROUND

[0002] At present, due to increasing life pressure, scalp and hair loss problems are more and more common. There are many shampoo products on the market, but most of them are mainly used for cleaning and hair care. There are also many anti-hair loss shampoo products, but many of them only make the hair smoother and reduce the pulling force when combing, so that the hair loss appears less. However, this is only a superficial phenomenon.

[0003] In order to prevent hair loss, surgical treatment or drug treatment methods can also be used, but the cost is high and the safety is poor. If the care is not proper and is stimulated, it may also cause hair loss again. SUMMARY

[0004] The purpose of the embodiments of the present application is to provide a scalp care anti-hair loss composition and application thereof, a shampoo product and a preparation method thereof.

[0005] The scalp is different from the skin of other parts of the body. It has a large number of large hair follicles that can produce long hair fibers, and each hair follicle is attached to a large sebaceous gland. In addition, the hair follicle is also closely connected with a complex nerve network and blood vessel network. Therefore, there are many reasons for hair loss. First, the sebaceous gland can store a considerable amount of sebum. In normal individuals, the sebaceous gland presents a complex secretion rhythm. The pores rich in sebum provide an aerobic environment for lipophilic microorganisms and parasites, such as propionibacteria, staphylococcus epidermidis, Malassezia, and helminth mites, which can cause inflammation of the scalp and also cause hair loss. Second, if the blood vessel network connected to the hair follicle has poor blood circulation, that is, the hair follicle is deficient in nutrients, which can also cause hair loss. Third, when the scalp has inflammation, it can also cause hair loss.

[0006] Therefore, the present application mainly performs daily care and repair of the scalp from various target points of the hair loss mechanism through the shampoo product in multiple dimensions, so as to make the scalp healthier and achieve the purpose of preventing hair loss.

[0007] In a first aspect, the present application provides a scalp care anti-hair loss composition, which comprises, by mass percentage:

[0008] Ginger juice 1.0% to 35%, allantoin 0.01% to 2.0%, panthenol 0.1% to 10.0%, cypress leaf extract 0.1% to 5%, hydrolyzed chitin 0.005% to 0.1%, salicylic acid 0.01% to 0.5%; and the balance is water.

[0009] The scalp care anti-hair loss composition provided in the application has synergistic effects among the components, and the components can be maximally utilized to care for the scalp from multiple pathways and multiple dimensions, so that the scalp is more nutritious and healthy, and the best ideal state of anti-hair loss effect is achieved, and finally the effect of preventing hair loss is achieved.

[0010] The scalp care anti-hair loss composition provided in the application can be applied to a shampoo product, so that the shampoo product can achieve the effect of hair care and anti-hair loss, thereby replacing conventional anti-hair loss chemical treatment drugs and medical treatment methods, and embodying the concept of convenience and health safety.

[0011] In other embodiments of the application, the method for using the scalp care anti-hair loss composition includes directly compounding the scalp care anti-hair loss composition in a shampoo product for use, and daily shampooing.

[0012] In other embodiments of the application, the scalp care anti-hair loss composition includes, by mass percentage:

[0013] ginger juice 5% to 20%, allantoin 0.05% to 1.0%, panthenol 0.2% to 5%, cypress leaf extract 0.3% to 3%, hydrolyzed chitin polysaccharide 0.01% to 0.08%, and salicylic acid 0.05% to 0.3%; and the balance is water.

[0014] In other embodiments of the application, the scalp care anti-hair loss composition includes, by mass percentage:

[0015] ginger juice 12%, allantoin 0.3%, panthenol 1.0%, cypress leaf extract 0.9%, hydrolyzed chitin polysaccharide 0.016%, salicylic acid 0.1%, and the balance is water.

[0016] In other embodiments of the application, the effective components in the ginger juice include gingerols, para-gingerone, zingerone, and ginger di-ketone.

[0017] In other embodiments of the application, the effective components in the cypress leaf extract include taxifolin, new sugi biflavone, oleinera biflavone, quercetin, quercitrin, myricetin, and kaempferol.

[0018] In other embodiments of the application, the scalp care anti-hair loss composition does not contain purslane extract.

[0019] In the second aspect, the application provides the use of the scalp care anti-hair loss composition provided in the first aspect in a shampoo product.

[0020] In a third aspect, the present application provides a shampoo product comprising the scalp care anti-hair loss composition provided in the first aspect.

[0021] In other embodiments of the present application, the shampoo product comprises:

[0022] 10-15 parts of the scalp care anti-hair loss composition;

[0023] 10-18 parts of ammonium laureth sulfate;

[0024] 1-5 parts of ammonium lauryl sulfate;

[0025] 1-6 parts of cocamidopropyl betaine;

[0026] 1-5 parts of dimethicone;

[0027] 0.8-1.5 parts of cocamide MEA;

[0028] 0.8-2.0 parts of ethylene glycol distearate;

[0029] 0.1-0.4 parts of guar hydroxypropyltrimonium chloride;

[0030] 0.1-0.5 parts of sodium chloride;

[0031] 0.05-0.2 parts of disodium EDTA.

[0032] In a fourth aspect, the present application provides a method for preparing a shampoo product, comprising:

[0033] adding uniformly dispersed guar hydroxypropyltrimonium chloride, disodium EDTA, and citric acid to a water bath pot at 80-85°C;

[0034] then adding ammonium laureth sulfate, ammonium lauryl sulfate, and cocamidopropyl betaine to the water bath pot and dissolving them uniformly;

[0035] then adding cocamide MEA and ethylene glycol distearate to the water bath pot and keeping it at temperature for 15-25 min;

[0036] then cooling it to below 45°C, adding dimethicone, sodium chloride, and the scalp care anti-hair loss composition to the water bath pot, and stirring until they are dissolved uniformly. BRIEF DESCRIPTION OF DRAWINGS

[0037] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings needed in the embodiments will be briefly introduced as follows. It should be understood that the following drawings only show some embodiments of the present application, and therefore should not be regarded as a limitation on the scope.

[0038] Figure 1A column chart showing the results of the zebrafish embryo dorsal aorta blood vessel (DA) relative width test is shown;

[0039] Figure 2 A column chart showing the results of the soothing efficacy test is shown;

[0040] Figure 3 A column chart showing the results of the repair efficacy test is shown;

[0041] Figure 4 A column chart showing the relative expression amount of zebrafish 5a-reductase genes is shown. DETAILED DESCRIPTION

[0042] In order to make the objectives, technical solutions, and advantages of the embodiments of the present application clearer, the technical solutions in the embodiments of the present application will be described clearly and completely below. Obviously, the described embodiments are only some of the embodiments of the present application, rather than all the embodiments of the present application.

[0043] Therefore, the following detailed description of the embodiments of the present application is not intended to limit the scope of the claimed application, but merely represents the selected embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative labor fall within the scope of the present application. The shampoo raw materials, reagents, and the like used in the present application are commercially available raw materials and reagents, unless otherwise specified.

[0044] The present application provides a scalp care anti-hair loss composition, which comprises, by mass percentage:

[0045] Ginger juice 1.0% to 35%, allantoin 0.01% to 2.0%, panthenol 0.1% to 10.0%, cypress leaf extract 0.1% to 5%, hydrolyzed chitin 0.005% to 0.1%, salicylic acid 0.01% to 0.5%; and the balance is water.

[0046] The scalp care anti-hair loss composition provided by the present application has synergistic effects among the components; the effects of the components are maximized, the scalp is cared in multiple dimensions from the scalp hair follicles, multiple pathways of scalp inflammation, and the scalp is more nourished, making the scalp healthier, thereby achieving the best ideal state of anti-hair loss effect, and ultimately achieving the effect of preventing hair loss.

[0047] The scalp care anti-hair loss composition of the present application can be applied to a shampoo product, so that the shampoo product can achieve a hair care anti-hair loss effect. Thus, the conventional anti-hair loss chemical treatment drugs and medical treatment methods can be replaced, embodying the concept of convenience, health, and safety.

[0048] In other embodiments of the present application, the method for using the scalp care anti-hair loss composition comprises: directly compounding the scalp care anti-hair loss composition in a shampoo product for use, and washing hair daily.

[0049] Further, in some embodiments of the present application, the scalp care anti-hair loss composition comprises, by mass percentage:

[0050] ginger juice 5% to 20%, allantoin 0.05% to 1.0%, panthenol 0.2% to 5%, cypress leaf extract 0.3% to 3%, hydrolyzed chitin 0.01% to 0.08%, salicylic acid 0.05% to 0.3%; and the balance is water.

[0051] Further, in some embodiments of the present application, the scalp care anti-hair loss composition comprises, by mass percentage:

[0052] ginger juice 5% to 20%, allantoin 0.05% to 1.0%, panthenol 0.2% to 5%, cypress leaf extract 0.3% to 3%, hydrolyzed chitin 0.01% to 0.08%, salicylic acid 0.05% to 0.3%; and the balance is water.

[0053] Further, in some embodiments of the present application, the effective ingredients in the ginger juice include: gingerols, shogaols, zingerone, and zingeridines.

[0054] In some embodiments of the present application, the ginger juice can be from fresh ginger.

[0055] Fresh ginger is the fresh rhizome of Zingiber officinale Roscoe. Gingerols, shogaols, zingerone, and zingeridines are effective ingredients in fresh ginger, and all of them contain a 3-methoxy-4-hydroxyphenyl functional group. Fresh ginger has anti-inflammatory and antifungal effects, and can be used to treat infections caused by Malassezia furfur and Pityrosporum ovale. The present application compounding ginger juice with other components to treat seborrhea and dandruff achieves a synergistic effect, effectively prevents hair loss, and inhibits scalp itching.

[0056] Further, in some embodiments of the present application, allantoin is an amphoteric compound that can bind to various substances to form complex salts, has the effects of light protection, bactericidal preservation, pain relief, and antioxidant, can keep the skin moist, nourishing and soft, and is an effective additive for cosmetics such as freckle cream, acne liquid, shampoo, soap, toothpaste, shaving lotion, hair conditioner, astringent, anti-sweating and deodorant lotion, etc. The cosmetics containing allantoin have the effects of protecting tissues, hydrophilic, water absorption, and preventing moisture loss, etc. The lip balm and face cream containing allantoin can make the skin and lips soft and elastic, and have a beautiful luster. Allantoin promotes tissue growth, cell metabolism, and softens keratin proteins. For example, the functions of softening keratin, moisturizing, and increasing luster of the magic care lip balm are mainly due to the effect of allantoin.

[0057] The present application creatively combines allantoin with other components to be used in hair products such as hair cream, hair paste, and shampoo, achieves a synergistic effect, protects the hair, and makes the hair not split and grow continuously.

[0058] Further, in some embodiments of the present application, panthenol can be used as a moisturizing agent to improve the water content of the stratum corneum, reduce TEWL, improve skin roughness, and soothe the skin after irritation. In addition, panthenol can activate fibroblast proliferation and accelerate epithelial cell regeneration, and thus can help wound healing.

[0059] The present application creatively combines panthenol with other components to be used in hair care, achieves a synergistic effect, can well enhance the toughness of the hair, reduce the damage caused by mechanical processes such as combing, and keep the moisture content of the hair to prevent the hair from becoming dry and frizzy.

[0060] Further, in some embodiments of the present application, the effective components in the extract of Platycladus orientalis include: cypress double flavones, new sugi double flavones, spikemoss double flavones, quercetin, quercitrin, myricetin, and kaempferol.

[0061] Further, in some embodiments of the present application, the main components of Platycladus orientalis are tannins and flavonoid compounds, which include cypress double flavones, new sugi double flavones, spikemoss double flavones, quercetin, quercitrin, myricetin, and kaempferol, among which quercitrin has the highest content. The tannins in Platycladus orientalis are tannic acid. The leaf of Platycladus orientalis also contains volatile oil components, the highest content of which is 2,2-tetramethyl-3,7-cyclodecadien-1-methanol and cedrol. In addition, the leaf of Platycladus orientalis also contains cypressene, thujaketone, a-pinene, β-pinene, terpilenyl acetate, isocarophyllene, and α-carophyllene, etc.

[0062] Further, in some embodiments of the present application, the extract of Platycladus orientalis has inhibitory effect on Staphylococcus aureus, Escherichia coli, Cateballus, Streptococcus A, Pneumococcus, Dysentery bacillus, Typhoid bacillus, etc., and has inhibitory effect on the growth of tubercle bacillus. The phenolic substance in the extract of Platycladus orientalis has anti-pathogenic fungal effect on human body, and can better inhibit the activity of candida. The extract of Platycladus has anti-inflammatory effect, as shown by the inhibition test on rat toe edema and the inhibition of urocanase.

[0063] The Platycladus extract is creatively compounded with other components in the scheme of the present application, which is used in hair washing and care products, realizes synergistic effect, and can improve hair follicles and inflammation of scalp.

[0064] Further, in some embodiments of the present application, the hydrolyzed chitin is a completely biodegradable natural organic matter, which is a deacetylation product of chitin and has certain bacteriostatic effect. It is an ideal bacteriostatic raw material, especially excellent in inhibiting Malassezia, and has certain conditioning effect without accumulation effect.

[0065] The hydrolyzed chitin is creatively compounded with other components in the scheme of the present application, which is used in hair washing and care products, realizes synergistic effect, and can improve hair follicles and inflammation of scalp.

[0066] Further, in some embodiments of the present application, salicylic acid can dissolve the interkeratin constituting material, so as to cause the keratin layer to fall off, thereby removing the accumulated keratin layer, promoting metabolism, and making the epidermal cells fresh and full of life. Salicylic acid is liposoluble, can penetrate into the deep layer of pores along the sebaceous glands, is beneficial to dissolve the old keratin layer accumulated in the pores, and can improve the situation of pore blockage, thereby blocking the formation of acne and reducing the enlarged pores. The salicylic acid acts on the hair follicle wall cells, can help to remove the blocked hair follicles, correct abnormal cell shedding, prevent the pores from being blocked, and is most effective for blackhead acne. It can reduce the abnormal shedding of hair follicle wall and prevent the generation of new lesions.

[0067] The salicylic acid is creatively compounded with other components in the scheme of the present application, which is used in hair washing and care products, realizes synergistic effect, and can improve hair follicles, promote hair follicle growth, and reduce hair follicle shedding.

[0068] Further, exemplarily, in some embodiments of the present application, the scalp care anti-hair loss composition comprises, by mass percentage:

[0069] Ginger juice 5%, 6%, 8%, 10%, 15%, 18%, 20%, or a range between any two of the foregoing values;

[0070] allantoin 0.05%, 0.06%, 0.08%, 0.1%, 0.5%, 0.6%, 0.8%, 1.0%, or a range between any two of the aforementioned values;

[0071] panthenol 0.2%, 0.3%, 0.5%, 0.8%, 1%, 2%, 3%, 4%, 5%, or a range between any two of the aforementioned values;

[0072] thuja extract 0.3%, 0.5%, 0.8%, 1%, 1.5%, 1.8%, 2%, 2.5%, 2.8%, 3%, or a range between any two of the aforementioned values;

[0073] hydrolyzed chitin 0.01%, 0.02%, 0.03%, 0.04%, 0.05%, 0.06%, 0.07%, 0.08%, or a range between any two of the aforementioned values;

[0074] salicylic acid 0.05%, 0.06%, 0.08%, 0.1%, 0.15%, 0.2%, 0.25%, 0.26%, 0.28%, 0.3%, or a range between any two of the aforementioned values;

[0075] a balance of water.

[0076] Further, in some embodiments of the present application, the scalp care anti-hair loss composition does not contain the extract of purslane.

[0077] In some embodiments of the present application, the method for preparing the scalp care anti-hair loss composition described above comprises: mixing and stirring the components described above uniformly, i.e. the scalp care anti-hair loss composition.

[0078] Further, in some embodiments of the present application, the use of the scalp care anti-hair loss composition provided by any of the aforementioned embodiments in a shampoo product is provided.

[0079] Some embodiments of the present application provide a shampoo product comprising the scalp care anti-hair loss composition provided by any of the aforementioned embodiments.

[0080] Further, in some embodiments of the present application, the shampoo product comprises:

[0081] 10-15 parts of the scalp care anti-hair loss composition;

[0082] 10-18 parts of ammonium laureth sulfate;

[0083] 1-5 parts of ammonium lauryl sulfate;

[0084] 1-6 parts of cocamidopropyl betaine;

[0085] 1 to 5 parts of dimethicone;

[0086] 0.8 to 1.5 parts of cocamide MEA;

[0087] 0.8 to 2.0 parts of ethylene glycol distearate;

[0088] 0.1 to 0.4 parts of guar hydroxypropyltrimonium chloride;

[0089] 0.1 to 0.5 parts of sodium chloride;

[0090] 0.05 to 0.2 parts of disodium EDTA.

[0091] Exemplarily, in some embodiments of the present application, the shampoo product comprises:

[0092] 10, 11, 12, 13, 14, or 15 parts of a scalp care anti-hair loss composition;

[0093] 10, 11, 12, 13, 14, 15, 16, 17, or 18 parts of ammonium laureth sulfate;

[0094] 1, 2, 3, 4, or 5 parts of ammonium lauryl sulfate;

[0095] 1, 2, 3, 4, 5, or 6 parts of cocamidopropyl betaine;

[0096] 1, 2, 3, 4, or 5 parts of dimethicone;

[0097] 0.8, 0.9, 1, 1.1, 1.2, 1.3, 1.4, or 1.5 parts of cocamide MEA;

[0098] 0.8, 0.9, 1, 1.1, 1.2, 1.3, 1.4, 1.6, 1.8, or 2.0 parts of ethylene glycol distearate;

[0099] 0.1, 0.2, 0.3, or 0.4 parts of guar hydroxypropyltrimonium chloride;

[0100] 0.1, 0.2, 0.3, 0.4, or 0.5 parts of sodium chloride;

[0101] 0.05, 0.06, 0.08, 0.1, 0.12, 0.15, 0.18, or 0.2 parts of disodium EDTA.

[0102] In other alternative embodiments of the present application, the above-mentioned shampoo product further comprises: 0.08 to 0.1 parts by weight of a preservative, 0.01 to 0.05 parts by weight of citric acid, 0.2 to 0.5 parts by weight of a fragrance; and the balance of water (supplemented with water to 100 parts by weight).

[0103] Some embodiments of the present application provide a method for preparing a shampoo product, comprising:

[0104] adding uniformly dispersed guar hydroxypropyltrimonium chloride, disodium EDTA, citric acid to a water bath pot at 80-85°C;

[0105] Then add lauryl polyoxyethylene sulfate ammonium, lauryl sulfate ammonium, cocamide propyl betaine to the water bath pot and dissolve uniformly;

[0106] Then add cocamide MEA, ethylene glycol distearate to the water bath pot and keep warm for 15-25 min;

[0107] Then cool down to below 45°C, add dimethicone, sodium chloride, and hair loss prevention composition with scalp care to the water bath pot and stir until dissolved uniformly.

[0108] Further, in some embodiments of the present application, the method for preparing a shampoo product comprises the following steps:

[0109] Step S1, adding uniformly dispersed guar hydroxypropyltrimonium chloride, disodium EDTA, citric acid to a water bath pot at 80-85°C.

[0110] Further, in some embodiments of the present application, uniformly dispersed guar hydroxypropyltrimonium chloride, disodium EDTA, and citric acid are added to a water bath pot at 80°C, 81°C, 82°C, 83°C, 84°C, or 85°C.

[0111] Illustratively, in some embodiments of the present application, after heating the water in the water bath pot to 80-85°C and keeping the temperature constant, then add guar hydroxypropyltrimonium chloride, disodium EDTA, and citric acid that have been previously dispersed in water.

[0112] Step S2, then add lauryl polyoxyethylene sulfate ammonium, lauryl sulfate ammonium, and cocamide propyl betaine to the water bath pot obtained in step S1 and dissolve uniformly.

[0113] Illustratively, in some embodiments of the present application, lauryl polyoxyethylene sulfate ammonium, lauryl sulfate ammonium, and cocamide propyl betaine are added to the water bath pot obtained in step S1 and completely dissolved.

[0114] Step S3, then add cocamide MEA and ethylene glycol distearate to the water bath pot obtained in step S2 and keep warm for 15-25 min.

[0115] Exemplarily, in some embodiments of the present application, the water bath obtained in step S2 is added with cocamide MEA, ethylene glycol distearate, and kept at a constant temperature for 15 min, 16 min, 17 min, 18 min, 19 min, 20 min, 21 min, 22 min, 23 min, 24 min or 25 min.

[0116] Step S4, then the water bath obtained in step S3 is cooled to below 45℃, and the water bath is added with dimethicone, sodium chloride, and a scalp care anti-hair loss composition; and stirred until dissolved uniformly.

[0117] Exemplarily, in some embodiments of the present application, the water bath obtained in step S3 is cooled to below 45℃, and the remaining dimethicone, sodium chloride, preservative and fragrance are added, and finally the scalp care anti-hair loss composition is added, and the stirring is continued for 15 minutes.

[0118] The present application adds a scalp care anti-hair loss composition to a shampoo product. The scalp care anti-hair loss composition in the present application repairs the scalp function through scalp care, making the scalp healthier, thereby achieving the effect of preventing or reducing hair loss.

[0119] Some specific examples are listed below to better illustrate the present application.

[0120] Examples

[0121] A scalp care anti-hair loss composition is provided, and the specific composition is shown in Table 1.

[0122] Table 1

[0123]

[0124] Comparative Examples

[0125] A composition is provided, and the specific composition is shown in Table 2.

[0126] Table 2

[0127]

[0128] The performance of the composition provided in each example or each comparative example is detected.

[0129] Experimental Examples

[0130] The composition provided in Examples 1-7 and Comparative Examples 1-7 is tested by a zebrafish test model in four dimensions of repair promotion rate, neutrophil aggregation resistance rate, 5α-reductase gene expression inhibition rate, and blood vessel expansion rate.

[0131] 1. Zebrafish embryo tail fin repair promotion test: Zebrafish embryo tail fin amputation model is used for testing. 24 tail fin amputated fish embryos are exposed to 0.01 g / L test sample solution, while a blank control group, a positive control group and a model control group are set. After 48 h of exposure, the fish embryos are photographed under a microscope, the tail fin regeneration length is measured and statistical analysis is performed.

[0132]

[0133] In the formula: S - the average value of the tail fin length of the fish embryos in the test treatment group;

[0134] M - the average value of the tail fin length of the fish embryos in the model control group;

[0135] B - the average value of the tail fin length of the fish embryos in the blank control group.

[0136] The average value and standard error of each test group are calculated, and the results are statistically processed and expressed as mean ± standard error. Statistical software is used to perform variance analysis on the data, and a two-tailed T test is performed on the tail fin length between the test group and the model control group to obtain the p value. p < 0.05 indicates a significant difference.

[0137] The higher the repair promotion rate, the better the repair promotion effect of the composition.

[0138] 2. Zebrafish 5α-reductase gene expression test: 36 5-day-old zebrafish are divided into 3 groups and exposed to 0.05 g / L test sample solution, while a blank control group is set. After 24 h of exposure, the zebrafish are subjected to RNA extraction, cDNA synthesis, and real-time PCR amplification. β-actin is used as the housekeeping gene, and Ct is used as the amplification result. The relative expression of 5α-reductase gene is calculated and statistically analyzed.

[0139]

[0140] In the formula: 2 -ΔΔCt - relative expression of the gene.

[0141] The average value and standard error of each test group are calculated, and the results are statistically processed and expressed as mean ± standard error. Statistical software is used to perform variance analysis on the data, and a two-tailed T test is performed on the relative expression of the target gene between the test group and the blank control group to obtain the p value. p < 0.05 indicates a significant difference.

[0142] The greater the 5α-reductase gene expression inhibition rate, the better the composition's ability to inhibit male hormones and reduce androgenic ability.

[0143] 3. Zebrafish embryo neutrophil aggregation inhibition test: The model of neutrophil aggregation caused by copper sulfate-induced damage to the lateral line region of the neural crest cells of zebrafish embryos was used for testing. Twenty-four fish embryos were exposed to 0.16 mg / L copper sulfate pentahydrate and 0.05 g / L test sample solution, and a blank control group, a positive control group, and a model control group were set up. After 40 min of exposure, the fish embryos were fixed and stained with Sudan black, the number of neutrophils in the lateral line region was counted, and statistical analysis was performed.

[0144]

[0145] In the formula:

[0146] S - the average number of neutrophils in the fish embryos of the test substance treatment group, in units of particles per fish (particles / fish);

[0147] M - the average number of neutrophils in the fish embryos of the model control group, in units of particles per fish (particles / fish);

[0148] B - the average number of neutrophils in the fish embryos of the blank control group, in units of particles per fish (particles / fish).

[0149] The average and standard error of each test group were calculated, and the results were expressed as mean ± standard error. Statistical software was used to perform analysis of variance on the data, and a two-tailed T test was performed on the number of neutrophils between the test substance group and the model control group to obtain the p value. p < 0.05 indicates a significant difference.

[0150] The higher the neutrophil aggregation resistance rate, the better the soothing performance of the composition.

[0151] 4. Zebrafish embryo vasodilation efficacy test: Twelve 3-day-old Fli1:EGFP transgenic zebrafish embryos were exposed to 0.01 g / L test sample solution, and a blank control group was set up. After 3 h of exposure, each site was photographed under a microscope, the width was measured, the average value was taken, and statistical analysis was performed. The dorsal aorta of the zebrafish embryo. Then use software to measure the width of the dorsal aorta of each fish embryo 5 times, take the average value, and perform statistical analysis.

[0152]

[0153] In the formula:

[0154] S - the average width of the dorsal aorta of the fish embryos in the test substance treatment group;

[0155] B - the average width of the dorsal aorta of the fish embryos in the blank control group;

[0156] The average and standard deviation of each test group were calculated, and the results were expressed as mean ± standard deviation. Statistical software was used to perform analysis of variance on the data, and a two-tailed T test was performed on the dorsal aortic blood vessel width between the test group and the blank control group to obtain the p value. A p value of less than 0.05 indicates a significant difference.

[0157] The higher the rate of blood vessel expansion, the better the composition's ability to expand blood vessels.

[0158] The results of the zebrafish four-dimensional test are shown in Table 3.

[0159] Table 3

[0160]

[0161]

[0162] The results of the zebrafish four-dimensional test are shown in Table 3.

[0163] As can be seen from the test results in Table 3, Examples 1-7 have certain promoting effects in the four dimensions of repair promotion rate, neutrophil aggregation resistance rate, 5α-reductase gene expression inhibition rate, and blood vessel expansion rate. It can be seen that the scheme of the present application can repair the scalp from multiple dimensions, make the scalp more nutritious, make the scalp healthier, achieve the best ideal state of anti-hair loss effect, and ultimately achieve the effect of preventing hair loss.

[0164] Comparing the test results of Comparative Examples 1-7 shows that the components of the examples have a synergistic effect, repair the scalp from multiple dimensions, make the scalp more nutritious, make the scalp healthier, achieve the best ideal state of anti-hair loss effect, and ultimately achieve the effect of preventing hair loss.

[0165] Compared with Example 4, the values of the four dimensions of repair promotion rate, neutrophil aggregation resistance rate, 5α-reductase gene expression inhibition rate, and blood vessel expansion rate of each comparative example are significantly lower than those of Example 4; it can be seen that the anti-hair loss effect of the comparative examples is significantly lower than that of the examples. This can indicate that the components of the composition of the present application synergistically cooperate, have a synergistic effect, repair the scalp from multiple dimensions, make the scalp more nutritious, make the scalp healthier, achieve the best ideal state of anti-hair loss effect, and ultimately achieve the effect of preventing hair loss. Further, comparing Comparative Example 7 with Example 4 of the present application shows that when other ingredients, such as purslane extract, are added to the composition of the present application, the efficacy of the composition of the present application is inhibited.

[0166] Therefore, the components of the composition of the present application synergistically cooperate, and the components cannot be arbitrarily added or reduced, otherwise it may have an inhibitory effect.

[0167] Application Example

[0168] The composition provided in Example 4 above was applied to a shampoo product as a sample treatment group to test the anti-hair loss effect of the shampoo product.

[0169] The shampoo formula of the composition provided in Example 4 is shown in Table 4 below.

[0170] Table 4

[0171] Water up to 100 g Laureth-9 11.0g Ammonium lauryl sulfate 8.0g Cocamidopropyl betaine 5.0g Dimethicone 4.0g Cocamide MEA 1.2g Ethylene glycol distearate 1.5g Guar hydroxypropyltrimonium chloride 0.3g Sodium chloride 0.3g Disodium EDTA 0.1g Preservative 0.1g Citric acid 0.05g Fragrance 0.5g The composition provided in Example 4 14.516g

[0172] A shampoo formula without the addition of the above-mentioned anti-hair loss composition with scalp care was used as a blank control example. The specific composition is shown in Table 5.

[0173] Table 5

[0174] Water up to 100 g Laureth-9 11.0g Ammonium lauryl sulfate 8.0g Cocamidopropyl betaine 5.0g Dimethicone 4.0g Cocamide MEA 1.2g Ethylene glycol distearate 1.5g Guar hydroxypropyltrimonium chloride 0.3g Sodium chloride 0.3g Disodium EDTA 0.1g Preservative 0.1g Citric acid 0.05g Fragrance 0.5g

[0175] Shampoo product preparation method:

[0176] In a water bath, water was heated to 80-85°C and then kept at a constant temperature. Then, guar gum hydroxypropyltrimonium chloride, disodium EDTA, and citric acid, which were previously dispersed in water, were added. Then, ammonium lauryl polyoxyethylene sulfate, ammonium lauryl sulfate, and cocamidopropyl betaine were added, and after complete dissolution, cocamide MEA and ethylene glycol distearate were added. After constant temperature for 20 minutes, the temperature was lowered to below 45°C, and the remaining dimethicone, sodium chloride, preservatives, and fragrance were added. Finally, the composition of Example 4 (or no addition for the application example) was added, and stirring was continued for 15 minutes.

[0177] The performance of the above-mentioned shampoo product was tested.

[0178] 1. Twenty volunteers (10 people for each formula, aged 25-60 years, with scattered, non-health-related hair loss and fine and lifeless hair) were tested with the samples of Example 4 and the blank control example, respectively, to test the anti-hair loss effect according to the evaluation method, and the final result was taken as the average of the scores. The shampoo was used twice a week for 12 weeks, and the anti-hair loss effect before and after 12 weeks of use was compared, and the improvement in hair loss of each volunteer was tracked and tested.

[0179] Test score standard: The score is divided into 1, 2, 3, 4, and 5 levels, where 1 is no improvement in hair loss, 2 is no obvious improvement in hair loss, 3 is slight improvement in hair loss, 4 is good improvement in hair loss, and 5 is obvious improvement in hair loss, with 0.1 points as an interval to assess the improvement. After 12 weeks of use, the average score of 10 people in each group was taken, and the results are shown in Table 6.

[0180] Table 6 Formulation and test results of shampoo provided by Example 4 and blank comparative example

[0181]

[0182] As can be seen from Table 6, the anti-hair loss composition for scalp care in Example 4 can achieve the same effect in shampoo, making the scalp healthier, improving the ideal condition of hair loss, and ultimately achieving the effect of preventing hair loss.

[0183] The anti-hair loss effect of the blank comparative example is significantly lower than that of the anti-hair loss effect of the scheme of Example 4.

[0184] 2. Zebrafish multi-dimensional test of Example 4 sample treatment group and blank comparative example.

[0185] (1) Zebrafish embryo dorsal aorta blood vessel (DA) relative width test: 12 3-day-old Fli1:EGFP transgenic zebrafish embryos were exposed to 0.01 g / L test sample solution, and a blank control group was set up. After 3h of exposure, each site was photographed under a microscope for width measurement, and the average value was taken and statistically analyzed. The dorsal aorta blood vessel of the zebrafish embryo. Then use software to measure the width of each zebrafish embryo dorsal aorta blood vessel 5 times, take the average value and statistically analyze.

[0186] The test results are shown in the attached Figure 1 .

[0187] As can be seen from Figure 1 : The vasodilation rate of the sample treatment group reached 14% and p<0.05, indicating that it can significantly dilate the aorta blood vessel of the zebrafish embryo, indicating that the sample group has the effect of dilating blood vessels.

[0188] (2) Soothing effect test: neutrophil number characterization: zebrafish embryo neutrophil aggregation inhibition test: a model of neutrophil aggregation caused by copper sulfate-induced zebrafish embryo lateral line area neural crest cell damage was used for testing. 24 fish embryos were exposed to 0.16 mg / L copper sulfate pentahydrate and 0.05 g / L test sample solution, while a blank control group, a positive control group and a model control group were set up. After 40 min of exposure, the fish embryos were fixed and stained with Sudan black, the number of neutrophils in the lateral line area was counted and statistically analyzed.

[0189] The test results are shown in the attached Figure 2 .

[0190] As can be seen from Figure 2 : The aggregation inhibition rate of the sample treatment group was reduced by 23% compared with the blank control group and p<0.05, and the sample treatment group could significantly inhibit the aggregation of neutrophils in zebrafish embryos, indicating that the sample group had a soothing effect.

[0191] (3) Repair efficacy test: using zebrafish tail fin relative length as an indicator: a zebrafish embryo tail fin amputation model was used for testing. 24 tail fin amputated fish embryos were exposed to 0.01 g / L test sample solution, while a blank control group, a positive control group and a model control group were set up. After 48 h of exposure, the fish embryos were photographed under a microscope, the tail fin regeneration length was measured and statistical analysis was performed.

[0192] The test results are shown in the attached Figure 3 .

[0193] From Figure 3 it can be seen that the zebrafish embryo tail fin repair promotion rate of the sample treatment group was 30% and p < 0.05, the sample treatment group could significantly promote the regeneration of zebrafish embryo tail fin, indicating that the sample had repair performance.

[0194] (4) Zebrafish 5α-reductase gene expression test: 36 5-day-old zebrafish were divided into 3 groups and exposed to 0.05 g / L test sample solution, while a blank control group was set up. After 24 h of exposure, the zebrafish were subjected to RNA extraction, cDNA synthesis, real-time PCR amplification, with β-actin as the housekeeping gene and Ct as the amplification result. The relative expression of 5α-reductase gene was calculated and statistical analysis was performed.

[0195] The test results are shown in the attached Figure 4 .

[0196] From Figure 4 it can be seen that the expression of srd5a1 and srd5a2b genes in the sample treatment group was lower, indicating that the inhibition rate of zebrafish 5α-reductase type I gene (srd5a1) expression and zebrafish 5α-reductase type II gene (srd5a2b) expression was higher than that of the blank control group, indicating that the sample had the performance of inhibiting androgen to reduce male alopecia.

[0197] In summary, the various performances of the sample of Example 4 of the present application were better than those of the blank control group, thus it can be shown that the scalp care anti-hair loss composition of the present application applied in shampoo can achieve the same effect as the composition, making the scalp healthier and the hair loss condition improved ideally, ultimately achieving the effect of preventing hair loss.

[0198] The above-described embodiments are part of the embodiments of the present application, rather than all the embodiments. The detailed description of the embodiments of the present application is not intended to limit the scope of the claimed application, but only represents selected embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative labor fall within the scope of protection of the present application.

Claims

1. A scalp care anti-hair loss composition characterized in that, The hair loss prevention composition with scalp care according to the application consists of the following ingredients in percentage by mass: ginger juice 12% to 20%, allantoin 0.5% to 1.5%, panthenol 1% to 8%, cypress leaf extract 0.9% to 3%, hydrolyzed chitin 0.016% to 0.08%, salicylic acid 0.1% to 0.4%; the balance being water. The hair loss prevention composition with scalp care according to the application does not contain the extract of purslane.

2. A scalp care anti-hair loss composition according to claim 1, characterized in that, The hair loss prevention composition with scalp care according to the application consists of the following ingredients in percentage by mass: ginger juice 12%, allantoin 0.5%, panthenol 1%, cypress leaf extract 0.9%, hydrolyzed chitin 0.016%, salicylic acid 0.1%, the balance being water.

3. The hair loss prevention composition with scalp care according to claim 1 or 2, wherein the ginger juice contains the following active ingredients: gingerols, paragingerone, zingerone, ginger dienone.

4. The hair loss prevention composition with scalp care according to claim 1 or 2, wherein the cypress leaf extract contains the following active ingredients: hinokiflavone, new sylvestroflavone, amentoflavone, quercetin, quercitrin, myricetin, kaempferol.

5. Use of the hair loss prevention composition with scalp care according to any one of claims 1 to 4 in a shampoo product. The shampoo product comprises the hair loss prevention composition with scalp care according to any one of claims 1 to 4. in parts by weight, 6. A shampoo product characterized in that, The shampoo product comprises:

7. The shampoo product according to claim 6, wherein 10 to 15 parts of the hair loss prevention composition with scalp care; 10 to 18 parts of ammonium laureth sulfate; 1 to 5 parts of ammonium lauryl sulfate; 1 to 6 parts of cocamidopropyl betaine; 1 to 5 parts of dimethicone; 0.8 to 1.5 parts of cocamide MEA; 0.8 to 2.0 parts of ethylene glycol distearate; 0.1 to 0.4 parts of guar hydroxypropyltrimonium chloride; 0.1 to 0.5 parts of sodium chloride; 0.05 to 0.2 parts of disodium EDTA. The shampoo product comprises: The guar hydroxypropyltrimonium chloride, disodium EDTA and citric acid are added to a water bath at 80 to 85°C; 8. The method of preparing a shampoo product according to claim 7, characterized in that, Then ammonium laureth sulfate, ammonium lauryl sulfate and cocamidopropyl betaine are added to the water bath and dissolved uniformly; Then cocamide MEA and ethylene glycol distearate are added to the water bath and kept at 80 to 85°C for 15 to 25 minutes; Then the temperature is lowered to below 45°C, dimethicone, sodium chloride and the hair loss prevention composition with scalp care are added to the water bath and stirred until dissolved uniformly. ​ ​

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