A pesticide composition containing Cyclobutrifluram and its application

Abstract

This invention belongs to the field of pesticide fungicide technology and discloses a pesticide composition containing Cyclobutrifluram. The pesticide composition comprises active ingredient A and active ingredient B, wherein active ingredient A is Cyclobutrifluram, and active ingredient B is either prothioconazole or flutriafol. The mass ratio of active ingredient A to active ingredient B is 1:50 to 50:1. The pesticide composition of this invention has a good control effect on plant diseases caused by Fusarium fungi, effectively controlling the spread and development of diseases and reducing the dosage of pesticides used.

Description

Technical Field

[0001] This invention belongs to the field of pesticide fungicide technology, and discloses a pesticide composition containing Cyclobutrifluram and its application. Background Technology

[0002] Prothioconazole is a triazole thiophene fungicide discovered by Bayer Crop Science. It belongs to the sterol demethylation (ergosterol biosynthesis) inhibitor class and possesses selectivity, protective, curative, and long-lasting effects. Due to its high activity, broad spectrum, and relatively safe properties, prothioconazole is widely used globally in crops such as wheat, soybeans, rapeseed, rice, peanuts, and sugar beets.

[0003] Epixaconazole is a triazole fungicide developed by BASF. It has a broad spectrum and long-lasting effect, and has a good fungicidal effect on a variety of pathogens in cereal crops.

[0004] Fusarium is a large genus of fungi. It is widely distributed in nature, living both parasitic and saprophytic lives. It is one of the most important plant pathogens discovered by humans. It can infect a variety of plants (food crops, oil crops, cash crops, medicinal plants, and ornamental plants), causing various types of rot diseases such as wilting, root rot, and ear rot, leading to severe yield reduction and significant economic losses. Some Fusarium species can also produce fungal toxins, which can cause food poisoning or even death if ingested by humans or animals.

[0005] Rice seedling blight is mainly caused by Fusarium moniliforme and Fusarium moniliforme, which can cause a loss of 3% to 70% of rice yield and also produce toxins.

[0006] Fusarium head blight (FHB) is a fungal disease caused by various Fusarium species, and can occur at any stage of wheat growth, from seedling to heading. FHB causes wheat ears to wilt, grains to become wrinkled and shrunken, and flour yield to decrease. Grains with milder infection show a sharp drop in germination rate, affecting seedling quality. Even if germination occurs, seedlings are particularly prone to death. Severely infected wheat grains should not be used for seed. Furthermore, FHB has a significant impact on wheat yield, as infected grains readily produce gibberellin, a toxin that can cause adverse symptoms such as dizziness, diarrhea, and vomiting.

[0007] Wheat stem rot is a fungal, soil-borne disease. The pathogen invades from the base of the wheat stem or roots, primarily damaging the 1-2 leaf sheaths and stem at the base, causing lodging and premature death, resulting in whiteheads. Yields are typically reduced by 5%-10%, but in severe cases, losses can exceed 50%, or even total crop failure. Currently, wheat varieties promoted in agricultural production have poor resistance to stem rot. Wheat straw carrying large amounts of pathogens is often returned to the field without sufficient decomposition, and the long-term excessive application of nitrogen fertilizers in some wheat fields has exacerbated the disease in recent years.

[0008] To develop new agents for the control of wheat scab, wheat stem rot, and rice bakanae disease, the inventors of this application used the mycelial growth rate method to test the in vitro toxicity of Cyclobutrifluram with prothioconazole, flutriafol, and Cyclobutrifluram with prothioconazole and flutriafol against the main pathogens of rice bakanae disease, wheat stem rot, and wheat scab. The combined action types of the mixtures were analyzed, and field trials were conducted to evaluate the efficacy and safety of the selected mixtures against wheat scab, wheat stem rot, and wheat bakanae disease. This provides more agent options for reducing pesticide use and increasing efficacy in controlling diseases caused by Fusarium and rice, as well as for managing pesticide resistance. Summary of the Invention

[0009] To address the above problems, this invention provides a pesticide composition containing Cyclobutrifluram. The active ingredient of this pesticide composition is a compound of Cyclobutrifluram and any one of prothioconazole and flutriafol. It has excellent control effects on diseases caused by Fusarium, especially showing significant synergistic effects against wheat scab, wheat stem rot and rice bakanae disease, slowing down the development of pathogen resistance and effectively reducing the amount of pesticide used.

[0010] The present invention also provides an application of a pesticide composition containing Cyclobutrifluram for the control of pathogenic Fusarium spp.

[0011] The present invention further provides the use of the pesticide composition for the prevention and control of wheat scab, rice bakanae disease and wheat stem rot.

[0012] To achieve the above objectives, the present invention adopts the following technical solution: a pesticide composition containing Cyclobutrifluram, wherein the pesticide composition comprises active ingredient A and active ingredient B, wherein active ingredient A is Cyclobutrifluram, and active ingredient B is either prothioconazole or flutriafol, and the mass ratio of active ingredient A to active ingredient B is 1:50 to 50:1.

[0013] Furthermore, the active ingredient B is prothioconazole, and the mass ratio of active ingredient A to active ingredient B is 1:35 to 40:1.

[0014] Furthermore, the active ingredient B is prothioconazole, and the mass ratio of active ingredient A to active ingredient B is 1:30 to 30:1.

[0015] Furthermore, the active ingredient B is flutriafol, and the mass ratio of active ingredient A to active ingredient B is 1:45 to 40:1;

[0016] Furthermore, the active ingredient B is flutriafol, and the mass ratio of active ingredient A to active ingredient B is 1:30 to 20:1.

[0017] Furthermore, based on a total weight of 100 wt%, the total weight of active ingredient A and active ingredient B accounts for 0.5% to 80% of the total weight of the pesticide composition;

[0018] Furthermore, in addition to the active ingredient, the pesticide composition also contains agriculturally permitted auxiliary ingredients, which are selected from one or more of the following: wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoamers, solvents, preservatives, stabilizers, synergists, binders, fillers, or carriers.

[0019] Furthermore, the wetting agent is selected from one or more of the following: sodium dodecyl sulfate, sodium dodecylbenzene sulfonate, pull-opening powder BX, wetting and penetrating agent F, soapberry powder, silkworm excrement, or soapberry powder;

[0020] Furthermore, the dispersant is selected from one or more of the following: polycarboxylate, lignin sulfonate, alkylphenol polyoxyethylene ether formaldehyde condensate sulfate, calcium alkylbenzene sulfonate, sodium naphthalene sulfonate formaldehyde condensate, alkylphenol polyoxyethylene ether, fatty amine polyoxyethylene ether, fatty acid polyoxyethylene ether, or glycerol fatty acid ester polyoxyethylene ether.

[0021] Furthermore, the emulsifier is selected from one or a mixture of multiple of the following: calcium alkylbenzene sulfonate, OP series phosphate esters (nonylphenol polyoxyethylene ether phosphate ester), phenylphenol polyoxyethylene ether phosphate ester, styrene polyoxyethylene ether ammonium sulfate, alkyl biphenyl ether magnesium disulfonate, triethanolamine salt, benzyl dimethylphenol polyoxyethylene ether, alkylphenol formaldehyde resin polyoxyethylene ether, phenethylphenol formaldehyde resin polyoxyethylene ether, phenethylphenol polyoxyethylene polypropylene ether, ethylene oxide-propylene oxide block copolymer, OP series (nonylphenol polyoxyethylene ether), castor oil polyoxyethylene ether, alkyl aryl polyoxyethylene polyoxypropylene ether, sorbitan monostearate, dehydrated sorbitan fatty acid ester polyoxyethylene ether, or fatty alcohol polyoxyethylene ether.

[0022] Furthermore, the thickener is selected from one or more of xanthan gum, polyvinyl alcohol, bentonite, carboxymethyl cellulose, or magnesium aluminum silicate;

[0023] Furthermore, the disintegrant is selected from one or more of the following: bentonite, urea, ammonium sulfate, aluminum chloride, low-substituted hydroxypropyl cellulose, lactose, citric acid, succinic acid, or sodium bicarbonate;

[0024] Furthermore, the antifreeze is selected from one or more of ethylene glycol, propylene glycol, glycerol, or urea, or a mixture thereof;

[0025] Furthermore, the defoamer is selected from silicone oil, silicone compounds, and C. 10 ~C 20 Saturated fatty acid compounds or C8-C 10 A mixture of one or more fatty alcohol compounds;

[0026] Further, the solvent is selected from one or more of the following: N,N-dimethylformamide, cyclohexanone, butyl ether, xylene, dimethyl sulfoxide, methanol, ethylene glycol, ethanol, propanol, butanol, trimethylcyclohexanone, N-octylpyrrolidone, toluene, ethanolamine, triethanolamine, isopropylamine, N-methylpyrrolidone, diethylene glycol, ethylene glycol methyl ether, ethyl acetate, or acetonitrile.

[0027] Furthermore, the stabilizer is selected from one or more of the following: epoxidized soybean oil, epichlorohydrin, BHT, ethyl acetate, and triphenyl phosphate;

[0028] Furthermore, the penetrant is selected from one or more of penetrant JFC, penetrant T, azone, or organosilicon;

[0029] Furthermore, the carrier is one, two, or three of the solvent or filler, and the water is preferably deionized water;

[0030] Furthermore, the filler is selected from one or more of the following: kaolin, diatomaceous earth, bentonite, attapulgite, silica, starch, or light calcium carbonate.

[0031] All of the above substances are commercially available;

[0032] Furthermore, the pesticide composition can be prepared into a pesticide-acceptable formulation, wherein the formulation is a suspension concentrate, emulsion, emulsifiable concentrate, soluble concentrate, water-dispersible granule, wettable powder, dispersible oil suspension, or seed treatment suspension.

[0033] Furthermore, the formulation is a suspension concentrate, emulsifiable concentrate, dispersible oil suspension, seed treatment suspension, water-dispersible granules, or wettable powder.

[0034] The present invention also discloses the application of the pesticide composition described above for the prevention and control of plant diseases caused by Fusarium fungi.

[0035] Furthermore, the diseases mentioned are wheat scab, rice bakanae disease, or wheat stem base rot.

[0036] The beneficial effects of this invention are as follows:

[0037] 1) This invention combines compounds with different mechanisms of action, which have significant preventive effects against plant diseases caused by Fusarium, effectively slowing down the occurrence and spread of the diseases;

[0038] 2) The pesticide composition of the present invention is safe for crops, slows down the development of pathogen resistance, and increases crop yield. Detailed Implementation

[0039] To make the technical solutions and advantages of the present invention clearer, the present invention is further illustrated by the following specific formulation preparation examples and embodiments. However, the scope of protection of the present invention should not be limited by the specific embodiments described herein.

[0040] Formulation preparation example:

[0041] Preparation Example 1: 30% Cyclobutrifluram·Prothioconazole suspension (1:5)

[0042] Formula composition: 5% Cyclobutrifluram, 25% Prothioconazole, 1% Sodium Dodecyl Sulfate, 1% Succinate Sulfonate, 3% Alkylphenol Polyoxyethylene Ether Phosphate, 0.25% Xanthan Gum, 1% Magnesium Aluminum Silicate, 4% Propylene Glycol, 0.1% Sodium Paraben, 0.5% Silicone Oil, Deionized Water to make up the balance;

[0043] Preparation method: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in the reaction vessel in sequence, water is added and mixed evenly, and then subjected to high-speed shearing, wet sand milling and finally homogenization filtration to obtain the suspension product.

[0044] Preparation Example 2: 20% Cyclobutrifluram·Prothioconazole EC (4:1)

[0045] Formula composition: 16% Cyclobutrifluram, 4% prothioconazole, 15% decanoamide, 12% styrene-phenol polyoxyethylene ether, 2% calcium dodecylbenzenesulfonate, 20% propylene carbonate, xylene to make up the balance.

[0046] Preparation method: According to the formula ratio, add the active ingredient, solvent and co-solvent into the mixing tank and stir to dissolve them. Then add the emulsifier, and use the remaining solvent to make up the balance. Stir evenly in the mixing tank, and filter to obtain the emulsifiable oil required by the present invention.

[0047] Preparation Example 3: 28% Cyclobutrifluram·Prothioconazole Dispersible Oil Suspension (1:3)

[0048] Formula composition: 7% Cyclobutrifluram, 21% prothioconazole, 5% fatty alcohol polyoxyethylene ether, 12% castor oil polyoxyethylene ether, 2% calcium dodecylbenzenesulfonate, 2% sodium polycarboxylate, 0.5% organic bentonite, methyl oleate to make up the balance;

[0049] Preparation method: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in the reaction vessel in sequence, oil is added and mixed evenly, and then subjected to high-speed shearing, wet sand milling and finally homogenization filtration to obtain the dispersible oil suspension product.

[0050] Preparation Example 4: 18% Cyclobutrifluram·Prothioconazole Seed Treatment Suspension (8:1)

[0051] Formula composition: 16% Cyclobutrifluram, 2% Prothioconazole, 1% Fatty alcohol polyoxyethylene ether, 5% Fatty alcohol polyoxyethylene ether sulfate, 2% Sodium polycarboxylate, 1% Polyacrylic acid, 0.25% Xanthan gum, 5% Rose red pigment, 5% Ethylene glycol, 1% Magnesium aluminum silicate, 0.5% Organosilicon defoamer, 0.45% Kathon, deionized water to make up the balance.

[0052] Preparation method: The active ingredients, additives and water are mixed and stirred evenly under high shear rate according to the formula, and then sanded for 2.5 hours in a sand mill to make the average particle size reach 1-5 micrometers, thus obtaining the seed treatment suspension.

[0053] Preparation Example 5: 20% Cyclobutrifluram·fluticasone suspension (2:3)

[0054] Formula composition: 8% Cyclobutrifluram, 12% Fluopyram, 1% Alkylphenol Polyoxyethylene Ether, 1% Sodium Lignosulfonate, 3% Fatty Alcohol Polyoxyethylene Ether Sulfate, 3% Alkyl Aryl Polyoxyethylene Ether Polyoxypropylene Ether, 0.5% Magnesium Aluminum Silicate, 0.20% Xanthan Gum, 4% Propylene Glycol, 0.2% Sodium Benzoate, 0.5% Silicone Oil, Deionized Water to make up the balance;

[0055] Preparation method: Same as in preparation example 1.

[0056] Preparation Example 6: 42% Cyclobutrifluram·fluconazole water-dispersible granules (5:1)

[0057] Formula composition: 35% Cyclobutrifluram, 7% Fluconazole, 10% Lignosulfonate, 4% Naphthalenesulfonate formaldehyde condensate, 2% Sodium dodecyl sulfate, 30% Starch, and Kaolin as a supplement.

[0058] Preparation method: According to the formula ratio, add the active ingredients to the carrier, and add surfactants and other functional additives to it. Mix, and after air jet pulverization, add 10-25% water. Then knead, granulate, dry and sieve to obtain water-dispersible granules; or spray water, granulate and dry the pulverized powder in a fluidized bed granulator, and then sieve to obtain the product.

[0059] Preparation Example 7: 49% Cyclobutrifluram·Fluorazole wettable powder (6:1)

[0060] Formula composition: 42% Cyclobutrifluram, 7% Flutriazole, 6% Sodium polycarboxylate, 5% NNO dispersant, 2% Sodium dodecyl sulfate, and kaolin to make up the balance.

[0061] Preparation method: According to the formula ratio, the active ingredients, dispersant, wetting agent and filler are mixed and stirred evenly in a stirring tank. The mixture is then pulverized and mixed evenly multiple times by an air jet mill to prepare the wettable powder of the composition of the present invention.

[0062] Preparation Example 8: 22% Cyclobutrifluram·fluticasone emulsifiable concentrate (1:10)

[0063] Formula composition: 2% Cyclobutrifluram, 20% Flutriazole, 20% Propylene Glycol Methyl Ether, 10% Fatty Alcohol Polyoxyethylene Ether, 3% Calcium Dodecylbenzene Sulfonate, 10% DMF, and methyl oleate to make up the balance.

[0064] Preparation method: Same as in preparation example 2.

[0065] All the preparations prepared in the above examples have been tested and found to meet the quality and technical indicators required for the corresponding preparations. The preparations obtained are qualified preparations recognized in this field.

[0066] Example 1: Indoor bioactivity assay of Cyclobutrifluram combined with prothioconazole or flutriafol as the pathogen of wheat scab. Test method: Indoor toxicity was determined by mycelial growth rate method.

[0067] Experimental material: The pathogen tested was Fusarium graminearum, the pathogen causing wheat scab.

[0068] Test culture medium: Potato glucose agar (PDA): 200g potato, 20g glucose, 18g agar, 1000mL water, natural pH. Used for the isolation, preservation, and indoor chemical testing of Fusarium graminearum, the causal agent of wheat blight.

[0069] Test reagents: 90% Cyclobutrifluram technical grade, 95% prothioconazole technical grade, 97% flutriafol technical grade.

[0070] Drug preparation: Dissolve the active ingredient in a suitable solvent and dilute with a 0.1% Tween 80 aqueous solution. Set up 5 series of mass concentration gradients for each drug treatment.

[0071] Drug preparation: Under aseptic conditions, pre-melted sterile culture medium was added to sterile Erlenmeyer flasks according to the experimental treatment. Quantitative amounts of drug solution were sequentially pipetted from low to high concentration and added to the respective Erlenmeyer flasks, then thoroughly mixed. Equal volumes were then poured into petri dishes to prepare agar plates containing the corresponding drug concentrations. A drug-free treatment was included as a blank control, and each treatment was repeated four times.

[0072] Indoor virulence determination: Under aseptic conditions, the cultured pathogens were punched along the edge of the colony using a 0.6 cm punch. The fungal cakes were then gently placed on the prepared drug-containing plates and incubated in a constant temperature incubator at 25°C. When the diameter of the control colony reached about 2 / 3 of the diameter of the petri dish, the diameter of the colony for each treatment was measured using the cross-crossing method, and the mycelial growth inhibition rate was calculated.

[0073] Data processing and computation:

[0074] According to statistics and analysis: Based on the survey results, the inhibition rate of mycelial growth of the tested target bacteria by each treatment concentration was calculated, in percentage (%). The calculation results were retained to two decimal places.

[0075] D = D1 - D2

[0076] In the formula:

[0077] D – Colony growth diameter;

[0078] D1—colony diameter;

[0079] D2 – Diameter of the mushroom cake.

[0080]

[0081] In the formula:

[0082] I – Mycelial growth inhibition rate;

[0083] D0—Correlation diameter of the blank control group;

[0084] D T — Diameter of colonies grown after chemical treatment.

[0085] The DPS statistical analysis system was used to analyze the virulence regression line and EC. 50 The value is used to evaluate the activity of the test reagent on the biological sample.

[0086] Sun Yunpei's method: The synergistic effect of drug mixtures is evaluated based on the co-toxicity coefficient (CTC). A CTC ≥ 120 indicates a synergistic effect; a CTC ≤ 80 indicates an antagonistic effect; and a CTC < 120 indicates an additive effect.

[0087] Calculation of the co-toxicity coefficient (CTC value) of the mixture:

[0088]

[0089] In the formula:

[0090] ATI – Actual Measured Toxicity Index of Mixtures;

[0091] S—EC of standard reagent 50 The unit is milligrams per liter (mg / L);

[0092] M – EC of the mixture 50 The unit is milligrams per liter (mg / L).

[0093] TTI = TI A ×P A +TI B ×P B

[0094] In the formula:

[0095] TTI – Theoretical Toxicity Index of Mixtures;

[0096] TI A —A. Toxicity index of drug A;

[0097] P A —Percentage content of drug A in the mixture, expressed as percentage (%);

[0098] TI B —Toxicity index of drug B;

[0099] P B —Percentage content of agent B in the mixture, expressed as percentage (%).

[0100]

[0101] In the formula:

[0102] CTC – Cotoxicity Coefficient;

[0103] ATI – Actual Measured Toxicity Index of Mixtures;

[0104] TTI – Theoretical Toxicity Index of Mixtures.

[0105] The test results are shown in the table below:

[0106] Table 1. Results of indoor bioactivity assay of Cyclobutrifluram combined with prothioconazole against wheat scab pathogen.

[0107]

[0108] Table 2. Results of the indoor bioactivity assay of Cyclobutrifluram combined with flutriafol against wheat scab pathogen.

[0109]

[0110]

[0111] Indoor bioactivity assays showed that a well-balanced combination of Cyclobutrifluram with prothioconazole or flutriafol exhibited excellent inhibitory effects on the mycelial growth of *Burkholderia bakanae*, the causal agent of rice seedling blight. When the mass ratio of Cyclobutrifluram to prothioconazole was 1:35–30:1, the co-toxicity coefficient was greater than 120, indicating a synergistic effect. Similarly, when the mass ratio of Cyclobutrifluram to flutriafol was 1:30–20:1, the co-toxicity coefficient was also greater than 120, demonstrating a synergistic effect.

[0112] Example 2: Indoor bioactivity assay of Cyclobutrifluram combined with prothioconazole or flutriafol against rice bakanae disease pathogen. Test method: The toxicity of the agent to the strain was determined by the indoor growth rate method.

[0113] Test strain: *Fusarium fujikuroi*, the pathogen of rice seedling blight.

[0114] Test culture medium: PDA medium, used for the culture, preservation and indoor virulence determination of rice bakanae disease pathogen.

[0115] Test reagents: 90% Cyclobutrifluram technical grade, 95% prothioconazole technical grade, 97% flutriafol technical grade.

[0116] Drug preparation: Dissolve the active ingredient in a suitable solvent and dilute with a 0.1% Tween 80 aqueous solution. Set up 5 series of mass concentration gradients for each drug treatment.

[0117] Drug preparation: Under aseptic conditions, according to the test treatment, add pre-melted sterile culture medium to a sterile Erlenmeyer flask. Take quantitative amounts of drug solution sequentially from low to high concentration and add them to the Erlenmeyer flasks, shaking thoroughly. Then pour equal volumes into petri dishes to prepare drug-containing plates of the corresponding concentrations.

[0118] Indoor virulence determination: The mycelial growth rate method was used. The preserved pathogen was transferred to PDA plates and activated at 26℃ for 72 h. Then, 0.6 cm diameter mycelial discs were cut near the colony edge using a punch and transferred to prepared drug-containing PDA plates. Drug-free PDA medium was used as a blank control. Each treatment was repeated four times. The plates were incubated at 26℃. When the colonies in the control plates grew to approximately 4 / 5 of the plate diameter, the colony diameter was measured using the cross-sectional method.

[0119] Data processing and computation:

[0120] Based on statistics and analysis: Based on the survey results, the inhibition rate of mycelial growth of the tested target bacteria by each treatment concentration was calculated, in percentage (%). The calculation results were retained to two decimal places.

[0121] D = D1 - D2

[0122] In the formula:

[0123] D – Colony growth diameter;

[0124] D1—colony diameter;

[0125] D2 – Diameter of the mushroom cake.

[0126]

[0127] In the formula:

[0128] I – Mycelial growth inhibition rate;

[0129] D0—Correlation diameter of the blank control group;

[0130] D T — Diameter of colonies grown after chemical treatment.

[0131] The DPS statistical analysis system was used to analyze the virulence regression line and EC. 50 The value is used to evaluate the activity of the test reagent on the biological sample.

[0132] Sun Yunpei's method: The synergistic effect of drug mixtures is evaluated based on the co-toxicity coefficient (CTC). A CTC ≥ 120 indicates a synergistic effect; a CTC ≤ 80 indicates an antagonistic effect; and a CTC < 120 indicates an additive effect.

[0133] Calculation of the co-toxicity coefficient (CTC value) of the mixture:

[0134]

[0135] In the formula:

[0136] ATI – Actual Measured Toxicity Index of Mixtures;

[0137] S—EC of standard reagent 50 The unit is milligrams per liter (mg / L);

[0138] M – EC of the mixture 50 The unit is milligrams per liter (mg / L).

[0139] TTI = TI A ×P A +TI B ×P B

[0140] In the formula:

[0141] TTI – Theoretical Toxicity Index of Mixtures;

[0142] TI A —A. Toxicity index of drug A;

[0143] P A —Percentage content of drug A in the mixture, expressed as percentage (%);

[0144] TI B —Toxicity index of drug B;

[0145] P B —Percentage content of agent B in the mixture, expressed as percentage (%).

[0146]

[0147] In the formula:

[0148] CTC – Cotoxicity Coefficient;

[0149] ATI – Actual Measured Toxicity Index of Mixtures;

[0150] TTI – Theoretical Toxicity Index of Mixtures.

[0151] The test results are shown in the table below:

[0152] Table 3. Results of the indoor bioactivity assay of Cyclobutrifluram combined with prothioconazole against rice bakanae disease pathogen.

[0153]

[0154] Table 4. Results of the indoor bioactivity assay of Cyclobutrifluram combined with flutriafol against rice seedling blight pathogen.

[0155]

[0156] Indoor bioactivity assays showed that Cyclobutrifluram, in combination with prothioconazole or flutriafol, exhibited good inhibitory effects on the mycelial growth of the pathogen causing rice bakanae disease. The combination of Cyclobutrifluram and prothioconazole at a mass ratio of 1:30–40:1 showed a synergistic effect; the combination of Cyclobutrifluram and flutriafol at a mass ratio of 1:45–30:1 also showed a synergistic effect.

[0157] Example 3: Field efficacy trial of pesticides for controlling wheat scab

[0158] Experimental location: Yanzhuang Village, Lichuan Town, Huixian County, Longnan City, Gansu Province. The soil fertility is moderate, and the previous crop was corn. The cultivation and management conditions of all experimental plots are uniform and consistent, which is in line with local scientific agricultural practices.

[0159] Experimental target: Wheat scab.

[0160] Experimental crop: wheat.

[0161] Experimental design: The experiment included 6 treatments, 5 chemical treatments, and 1 water control. The experimental plots were arranged in a randomized block design.

[0162] Application time: The experiment was conducted once during the wheat flowering stage (April 28, 2022).

[0163] Experimental survey: The experiment was conducted during the wheat grain-filling stage (May 24, 2022). Five samples were taken from each plot, and 100 ears were surveyed at each point. The ears were graded according to the percentage of dead ears to the total ear area, and the number of diseased ears at each grade and the total number of ears were recorded.

[0164] Grading method:

[0165] Grade 0: Disease-free entire ear of grain;

[0166] Grade 1: The area of ​​dead ears accounts for less than 1 / 4 of the total ear area;

[0167] Grade 3: The area of ​​dead ears accounts for 1 / 4 to 1 / 2 of the total ear area;

[0168] Grade 5: The area of ​​dead ears accounts for 1 / 2 to 3 / 4 of the total ear area;

[0169] Level 7: The area of ​​dead ears accounts for more than 3 / 4 of the total ear area.

[0170] Methods for calculating drug efficacy:

[0171]

[0172]

[0173]

[0174] Results and analysis of efficacy tests:

[0175] Table 5 Results of field efficacy trials for controlling wheat scab.

[0176] Drug Name <![CDATA[Application rate of active ingredient (g / hm 2 )]]> Disease index Prevention and control efficacy (%) 30% Cyclobutrifluram·Prothioconazole Suspension (1:5) 80 1.35 91.95 42% Cyclobutrifluram·Fluorazole water-dispersible granules (5:1) 80 2.06 87.73 480g / L prothioconazole suspension 112.5 3.22 80.79 30% Flutriazole Suspension 112.5 4.09 75.64 25% Cyclobutrifluram water dispersible granules 100 4.21 74.91 Blank control / 16.77 -

[0177] Field efficacy trials showed that combining Cyclobutrifluram with either prothioconazole or flutriafol in a reasonable ratio effectively controlled wheat scab.

[0178] Example 4: Field efficacy trial of pesticides for controlling rice bakanae disease

[0179] Experimental site: Dangli Village, Hengshanqiao Town, Changzhou City, Jiangsu Province. The experimental site has flat terrain and moderate soil organic matter content.

[0180] The test crop was rice (Wuyunjing 24), a rice variety that was naturally infected with rice bakanae disease.

[0181] Experimental target: Rice bakanae disease.

[0182] Experimental design: A total of 6 soaking treatments were designed for the experiment, and the test agents were used to soak the seeds according to the experimental design.

[0183] Soaking treatment: According to the experimental design, the rice seeds were soaked at room temperature for 60 h, and then placed at 28℃ for dark germination for 48 h.

[0184] Seedling raising treatment: Rice seeds for each treatment were sown into seedling trays for seedling raising. Each seedbed plot was 2.5m² in size. 2 Randomized block designation. Each block is approximately 0.1m in size. 2 Single-seed planting areas (200 seeds / plot) were set up within the area to investigate the germination rate.

[0185] Transplanting: When the seedlings are 30 days old, transplant them manually to the field, with each plot covering an area of ​​18m². 2 The rice was randomly assigned to a block design. Throughout the rice growing season, fertilization, irrigation, and other pest and disease management were carried out according to local practices.

[0186] Efficacy investigation of rice bakanae disease control: During the seedling stage (20 days old), five diagonal sampling points were taken from each treatment, with 100 seedlings surveyed at each point. The number of diseased seedlings was investigated, and the disease incidence and control effect were calculated. During the heading stage, five random sampling points were taken from each plot, with 10 clumps at each point. The total number of seedlings and the number of diseased seedlings were investigated, and the disease incidence and control effect of each treatment were calculated.

[0187] Methods for calculating drug efficacy:

[0188]

[0189]

[0190]

[0191] Experimental results and analysis:

[0192] Table 6 Results of field efficacy trials for controlling rice seedling disease.

[0193]

[0194] The field efficacy trials showed that both compound treatments were more effective than the single-agent control in controlling rice bakanae disease during both the seedbed and field stages. In terms of emergence rate, all treatments had higher emergence rates than the control group.

[0195] Example 5: Field efficacy test for controlling wheat stem base rot

[0196] Experimental site: Wheat fields in Dingzhuang Village, Beisu Town, Zoucheng City, Jining City, Shandong Province. The experimental area is planted with wheat year-round, and straw return to the field is a serious problem. The previous crop was corn. The terrain is relatively flat and the soil fertility is moderate.

[0197] Experimental crop: Wheat (Shannong 32).

[0198] Experimental target: Wheat stem rot.

[0199] Experimental Methods: Eight treatments were included in the experiment. Wheat seeds were treated with the respective treatment agents, then dried in a cool, shaded place before sowing. Field plots were arranged in a randomized block design, with each plot measuring 20 m². 2 Each treatment was repeated four times, and other agricultural measures in each community remained consistent.

[0200] Field survey for stem base rot control: A survey of wheat stem base rot was conducted during the milk stage of wheat. Five diagonal sampling points were used in each plot, and 100 plants were surveyed at each point. The severity of wheat disease was graded and recorded according to the following grading method, and the disease index and control effect were calculated.

[0201] The condition is classified according to the following criteria:

[0202] Grade 0: No browning symptoms observed throughout the plant;

[0203] Grade 1: The roots show signs of browning;

[0204] Grade 3: The first node of the above-ground part of the stem shows signs of browning and rotting;

[0205] Level 5: The second stem node above ground shows signs of browning and rotting;

[0206] Grade 7: Lesions extend beyond the second internode, but there are no white ears;

[0207] Grade 9: Disease spots extend beyond the second stem node, with white ears.

[0208] Formulas for calculating disease index and prevention and control effectiveness:

[0209]

[0210]

[0211] Wheat yield measurement: During the wheat harvest period, 5 points were selected in each treatment plot, and 1m was taken at each point. 2 Twenty ears of wheat were randomly selected to investigate the number of grains per ear, and the weight of 1,000 grains per ear was weighed.

[0212] Experimental Results and Analysis:

[0213] Table 7 Results of field efficacy trials for controlling wheat stem rot

[0214]

[0215] As shown in Table 7, the results of the experiment indicate that after seed treatment with the tested compound pesticides, the stem rot disease on wheat was reduced to varying degrees, and the yield components such as the number of grains per ear and the thousand-grain weight were improved compared with the blank control, which resulted in a significant increase in yield.

[0216] Although this application describes specific embodiments in detail with the aid of examples, the disclosure of this application can be modified and substituted in various ways. However, it should be understood that the disclosure of this application is not limited to the specific form disclosed. Rather, the disclosure of this application covers all modifications, equivalents, and substitutions within the scope of the disclosure of this application, the scope of which is defined by the appended claims and their legal equivalents.

Claims

1. A pesticide composition containing Cyclobutrifluram, characterized in that, The pesticide composition comprises active ingredient A and active ingredient B, wherein active ingredient A is Cyclobutrifluram and active ingredient B is prothioconazole, and the mass ratio of active ingredient A to active ingredient B is 1:30 to 30:

1.

2. The pesticide composition according to claim 1, characterized in that, The mass ratio of active ingredient A to active ingredient B is 1:30, 1:20, 1:15, 1:10, 1:8, 1:5, 1:3, 3:1, 4:1, 5:1, 7:1, 8:1, 10:1, 15:1, 20:1, or 30:

1.

3. The pesticide composition according to claim 1, characterized in that, The total weight of the pesticide composition is calculated as 100 wt%, and the total weight of active ingredient A and active ingredient B accounts for 0.5% to 80% of the total weight of the pesticide composition.

4. The pesticide composition according to claim 1, characterized in that, In addition to the active ingredient, the pesticide composition contains agriculturally permitted auxiliary ingredients, which are selected from one or more of the following: wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoamers, solvents, preservatives, stabilizers, synergists, binders, fillers, or carriers.

5. The pesticide composition according to claim 1, characterized in that, The pesticide composition is prepared into a pesticide-acceptable formulation, wherein the formulation is a suspension concentrate, a dispersible oil suspension concentrate, a water emulsion, an emulsifiable concentrate, a soluble concentrate, a water-dispersible granule, a wettable powder, or a seed treatment suspension concentrate.

6. The application of the pesticide composition according to any one of claims 1-5 for the control of plant diseases caused by Fusarium fungi, characterized in that, The diseases mentioned are wheat scab, rice bakanae disease, or wheat stem base rot.

Images