A method for controlling the pomponia operculella

Abstract

The present application belongs to the technical field of pest control, and particularly relates to a method for preventing and treating leptopharae of guavas. A kind of insecticidal composition is prepared by compounding effective component I and effective component II; the effective component I is pyridalyl, and the effective component II is acetoprole, emodin or spirodines. The mass ratio of the effective component I and the effective component II is 1-100:100-1. The method for preventing and treating leptopharae of guavas sprays the insecticidal composition on the leaves, and simultaneously, the guava fruits are bagged, so that the leptopharae of guavas can be effectively prevented and controlled. When the effective components in the insecticidal composition are compounded, the synergistic effect on the leptopharae of guavas is achieved, compared with a single effective component, the prevention and control effect on the leptopharae of guavas can be improved, the use dose of pesticides can be reduced, the influence on the environment can be reduced, the generation and development of drug resistance can be delayed, the service life of the pesticides can be prolonged, and the comprehensive prevention and control of agricultural pests is beneficial.

Description

Technical Field

[0001] This invention belongs to the field of pest control technology, specifically a method for controlling the guava leafroller. Background Technology

[0002] The horned leafroller (Spilonotarhothia), belonging to the family Spilonidae in the order Lepidoptera, is a pest that damages guava. The larvae can damage guava shoots and young fruits year-round. Adult females lay eggs on the undersides of leaves on new shoots. After hatching, the larvae feed on and damage the epidermis. After 3-4 days, they begin to spin silk to roll up new leaves or bind several leaves together, hiding inside to feed on the leaf tissue. A single insect can have several nests, and larvae can communalize or exchange nests. When disturbed, they push backward or spin silk to escape. Older larvae spin loose, thin cocoons and pupate inside. In high densities, there may be overlapping of generations. Damaged guava shoots stop developing, significantly impacting plant growth. Besides feeding on new shoots and leaves, mature larvae can also feed on flower spikes and the epidermis of young fruits, causing large brown scars on the fruit surface and inducing fruit diseases, severely affecting the commercial value and yield of the fruit.

[0003] In agricultural production, chemical pesticides can be used to control the horned leafroller on guavas, such as 50% chlorpyrifos EC at a dilution of 2000 times, 40% profenofos EC at a dilution of 2000 times, or 20% high-efficiency cypermethrin Emulsifiable Concentrate at a dilution of 2000 times. However, due to the repeated and large-scale use of these pesticides, the horned leafroller has gradually developed resistance, leading to a year-on-year decrease in the control efficacy of various existing pesticides against the horned leafroller, while the resistance problem is also gradually worsening. Therefore, screening new and highly effective compound formulations for controlling the guava horned leafroller is of great significance.

[0004] Acetaminophen, also known as trifluralin, is a highly effective and low-toxicity insecticide developed by Sumitomo Chemicals of Japan, primarily used to control lepidopteran larvae. Studies have shown that the effect of acetamiprid is highly correlated with its inhibitory effect on insect ovarian cells. Combining different pesticides is a common method for controlling agricultural pests, diseases, and weeds. Screening appropriate pesticide combinations can effectively improve actual control efficacy and delay the development of pesticide resistance in pests and weeds, making it an important means of integrated pesticide management. Through research on the combination of acetamiprid with other pesticides, the inventors discovered that acetamiprid has a synergistic effect against the leafroller moth when combined with acetaminophen, emodin, or pendimethalin. Currently, there are no reports of acetamiprid being combined with acetaminophen, emodin, or pendimethalin.

[0005] The information disclosed in this background section is intended only to enhance the understanding of the overall background of the invention and should not be construed as an admission or in any way implying that the information constitutes prior art known to those skilled in the art. Summary of the Invention

[0006] The purpose of this invention is to provide a method for controlling the guava leafroller, which involves using an insecticidal composition with synergistic effects for foliar spraying and bagging the guava fruit, which can effectively control the guava leafroller.

[0007] To achieve the above objectives, the present invention provides the following technical solution:

[0008] An insecticidal composition comprising a binary compound of active ingredient I and active ingredient II; wherein active ingredient I is acetamiprid, and active ingredient II is acetamiprid, emodin, or pendimethalin; wherein the mass ratio of active ingredient I to active ingredient II is 1-100:100-1.

[0009] Preferably, the active ingredient II is acetamiprid, and the mass ratio of active ingredient I to active ingredient II is 1-3:15-1.

[0010] Preferably, the active ingredient II is emodin, and the mass ratio of active ingredient I to active ingredient II is 1-20:20-1.

[0011] Preferably, the active ingredient II is pendimethalin, and the mass ratio of the active ingredient I to the active ingredient II is 1-5:10-1.

[0012] A second objective of this invention is to provide an insecticide comprising the aforementioned insecticidal composition. Those skilled in the art, given the known components of an insecticidal composition, can prepare insecticides of different formulations by selecting suitable auxiliary components. These auxiliary components can be selected from one or more of wetting agents, dispersants, fillers, antifreeze agents, defoamers, disintegrants, solvents, binders, and preservatives.

[0013] A third object of the present invention is to provide the application of the insecticidal composition in the control of the guava leafroller.

[0014] Finally, the present invention also provides a method for controlling the guava leafroller, specifically: spraying the insecticide mentioned above once every 7 days for 2 consecutive times; at the same time, bagging the guava fruit and sealing the bag opening tightly with the fruit stalk to reduce the number of leafroller larvae that can enter and damage the guava fruit.

[0015] Compared with the prior art, the present invention has the following beneficial effects:

[0016] The control method of this invention uses an insecticidal composition for foliar spraying, while simultaneously bagging the guava fruit, which can effectively control the guava leafroller. When the active ingredients in the insecticidal composition are combined, they have a synergistic effect against the guava leafroller. Compared with a single active ingredient, this method can improve the control effect against the guava leafroller, reduce the dosage of pesticides used, and reduce environmental impact. Furthermore, it can delay the development and spread of pesticide resistance, extend the lifespan of the pesticide, and is beneficial for integrated pest management. Detailed Implementation

[0017] The present invention can be better understood from the following embodiments. The description of the embodiments is for illustrative purposes only and should not and will not limit the present invention as described in detail in the claims.

[0018] Example Indoor bioactivity test of acetamiprid binary compound:

[0019] Experimental subject: Spironotarhothia leafroller, collected from a guava plantation, and continuously reared for multiple generations in the laboratory. Healthy 3rd instar larvae were selected as experimental subjects.

[0020] Test reagents: 91% acetamiprid technical grade (Shandong Hailier Chemical Co., Ltd.), 95% acetamiprid technical grade (Shenzhen Aituo Chemical Co., Ltd.), 95% emodin (Chengdu Ruifensidedan Biotechnology Co., Ltd.), 96% pendimethalin technical grade (Hefei Xingyu Chemical Co., Ltd.)

[0021] Test method: (Refer to "NY / T1154.6-2006 Guidelines for Indoor Bioassay of Pesticides - Part 6: Insecticidal Activity Test - Immersion Method")

[0022] 1. Dissolve the test reagent in dimethyl sulfoxide to prepare a single-agent stock solution, then dilute it with 0.1% Tween-80 aqueous solution. Set up multiple ratios, and set up 7 gradient mass concentrations for each single agent and each ratio mixture according to the proportional method, for later use.

[0023] 2. Immerse the test insects in the drug solution for 8 seconds, absorb excess solution with filter paper, and then transfer them to normal conditions for continued rearing with fresh, clean guava shoots and leaves. Each treatment is repeated 4 times, with 20 insects immersed in each replicate. A treatment with 0.1% Tween-80 aqueous solution is included as a blank control. Observe the mortality of the test insects 24 hours after treatment, record the total number of insects and the number of dead insects for each treatment, and calculate the corrected mortality rate for each treatment.

[0024]

[0025] In the above formula: P -- mortality rate, in %; K -- number of dead insects; N -- total number of insects treated.

[0026]

[0027] In the above formula: P1 -- corrected mortality rate, in %; P t --Treatment mortality rate, in %; P0--Control mortality rate, in %.

[0028] 3. Using DPS software, regression analysis was performed on the logarithmic values ​​of the drug concentrations for each treatment and the corrected mortality probability values ​​for each treatment to calculate the LC ratio of the drug for each treatment. 50 Then, the co-toxicity coefficient (CTC value) of the mixture was calculated according to Sun Yunpei's method, and the synergistic effect of the agent was evaluated based on the calculated co-toxicity coefficient (CTC). CTC≤80 indicates antagonistic effect, 80<CTC<120 indicates additive effect, and CTC≥120 indicates synergistic effect. The results are shown in Table 1-3.

[0029] Table 1. Indoor bioactivity assay of acetamiprid and acetamiprid in the horned leafroller.

[0030] Drug Name and Proportion <![CDATA[LC 50 (mg / L)]]> ATI TTI CTC Acetaminophen 5.7964 100.0000 -- -- Acetaminophen 16.1341 35.9264 -- -- Acetaminophen 1: Acetaminophen 15 7.1090 81.5361 39.9310 204.1925 Acetaminophen 1: Acetaminophen 10 6.3460 91.3394 41.7513 218.7704 Acetaminophen 1: Acetaminophen 5 4.4789 129.4157 46.6053 277.6844 Acetaminophen 1: Acetaminophen 3 9.0947 63.7338 51.9448 122.6953 Acetaminophen 1: Acetaminophen 1 5.6974 101.7376 67.9632 149.6952 Acetaminophen 3: Acetaminophen 1 4.5060 128.6374 83.9816 153.1733

[0031] As shown in Table 1, within a mass ratio of 1-3:15-1, the co-toxicity coefficients of acetamiprid and acetamiprid against the leafroller moth are both greater than 120, indicating a synergistic effect.

[0032] Table 2. Indoor bioactivity assay of acetamiprid and emodin combination in the leafroller moth.

[0033] Drug Name and Proportion <![CDATA[LC 50 (mg / L)]]> ATI TTI CTC Acetaminophen 5.7964 100.0000 -- -- emodin 84.5526 6.8554 -- -- Acetaminophen 1: Emodin 20 32.8887 17.6243 11.2908 156.0938 Acetaminophen 1: Emodin 15 25.0160 23.1708 12.6769 182.7792 Acetaminophen 1: Emodin 10 16.7065 34.6955 15.3231 226.4264 Acetaminophen 1: Emodin 5 7.3052 79.3462 22.3795 354.5490 Acetaminophen 1: Emodin 3 4.4407 130.5290 30.1415 433.0535 Acetaminophen 1: Emodin 1 8.5738 67.6060 53.4277 126.5373 Acetaminophen 3: Emodin 1 4.9072 118.1203 76.7138 153.9752 Acetaminophen 5: Emodin 1 1.3626 425.3926 84.4759 503.5669 Acetaminophen 10: Emodin 1 4.5584 127.1587 91.5323 138.9222 Acetaminophen 15: Emodin 1 3.3630 172.3580 94.1785 183.0121 Acetaminophen 20: Emodin 1 5.0314 115.2045 95.5645 120.5515

[0034] As shown in Table 2, within a mass ratio of 1-20:20-1, the co-toxicity coefficients of acetamiprid and emodin against the leafroller moth are both greater than 120, indicating a synergistic effect.

[0035] Table 3. Indoor bioactivity assays of acetamiprid and acetamiprid in the treatment of leafroller moth.

[0036] Drug Name and Proportion <![CDATA[LC 50 (mg / L)]]> ATI TTI CTC Acetaminophen 5.7964 100.0000 -- -- Acetaminophen 13.0208 44.5165 -- -- Acetaminophen 1: Acetaminophen 10 3.9506 146.7220 49.5604 296.0467 Acetaminophen 1: Acetaminophen 5 2.2278 260.1849 53.7637 483.9415 Acetaminophen 1: Acetaminophen 3 1.0239 566.1100 58.3873 969.5764 Acetaminophen 1: Acetaminophen 1 3.1918 181.6029 72.2582 251.3248 Acetaminophen 3: Acetaminophen 1 5.5226 104.9578 86.1291 121.8610 Acetaminophen 5: Acetaminophen 1 4.9425 117.2767 90.7527 129.2266

[0037] As shown in Table 3, within a mass ratio of 1-5:10-1, the co-toxicity coefficients of acetamiprid and acetamiprid against the leafroller moth are both greater than 120, indicating a synergistic effect.

[0038] In summary, when the active ingredients in the insecticidal composition of the present invention are combined, they have a synergistic effect on the guava leafroller. Compared with a single active ingredient, it can improve the control effect on the guava leafroller, reduce the dosage of pesticides, and reduce the impact on the environment.

[0039] The foregoing description of specific exemplary embodiments of the invention is for illustrative and explanatory purposes. These descriptions are not intended to limit the invention to the precise forms disclosed, and it will be apparent that many changes and variations can be made in accordance with the foregoing teachings. The exemplary embodiments were chosen and described in order to explain the specific principles of the invention and its practical application, thereby enabling those skilled in the art to implement and utilize various different exemplary embodiments of the invention, as well as various different choices and variations. The scope of the invention is intended to be defined by the claims and their equivalents.

Claims

1. An insecticidal composition, characterized in that, The insecticidal composition is a binary compound of active ingredient I and active ingredient II; active ingredient I is acetamiprid, and active ingredient II is acetamiprid; the mass ratio of active ingredient I to active ingredient II is 1-3:15-1.

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