Method for promoting eucalyptus seedling rooting by using water extract of gueldenstaedtia arborescens
By treating eucalyptus seedlings with micrantha aqueous extract, the problems of micrantha spread and eucalyptus root growth were solved, resulting in a significant improvement in root growth and resource utilization, reduced costs, and no environmental pollution.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- GUANGXI UNIV
- Filing Date
- 2024-02-19
- Publication Date
- 2026-04-24
AI Technical Summary
Existing technologies lack effective methods to control the rapid spread of Mikania micrantha and to utilize its resources, and have failed to significantly promote the root growth of eucalyptus seedlings, thus affecting their growth quality and afforestation benefits.
Eucalyptus seedlings were treated with water extracts of Mikania micrantha. The Mikania micrantha powder was dried, crushed, dissolved, and shaken to prepare water extracts of different concentrations, which were then irrigated onto the eucalyptus seedlings to promote root growth.
It significantly increases the root surface area, average root diameter, and total root length of eucalyptus seedlings, reduces production costs, is environmentally friendly, and provides a new direction for the resource utilization of Mikania micrantha.
Smart Images
Figure CN117814062B_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of plant cultivation technology, specifically to a method for promoting root growth in eucalyptus seedlings using a water extract of Mikania micrantha. Background Technology
[0002] Mikania micrantha, a plant belonging to the genus Eupatorium in the family Asteraceae, is an invasive alien weed in southern my country. It exhibits strong allelopathic activity, readily forming dominant populations and expanding rapidly. As an invasive species, Mikania micrantha is rich in resources and has broad prospects for development and utilization. Its allelopathic effects effectively enrich nitrogen-fixing and ammonifying bacteria, accelerating nutrient cycling in the rhizosphere. Simultaneously, it promotes the mineralization of soil nutrients such as nitrogen, phosphorus, and potassium through its allelopathic substances. Therefore, Mikania micrantha plays a crucial role in maintaining soil nutrient balance. In improving soil and the ecological environment, it can invade by altering soil nutrient structure and restoring site conditions. It can also adsorb heavy metals, addressing heavy metal pollution in the soil and becoming an important plant for environmental purification. Furthermore, its abundant allelopathic and antibacterial substances can be utilized in the production of antibacterial agents, insecticides, herbicides, and biopesticides to control other harmful organisms.
[0003] Eucalyptus, belonging to the Myrtaceae family, is one of the three major fast-growing timber species in my country. Eucalyptus is characterized by its rapid growth, high yield, excellent timber quality, wide range of uses, strong resistance to adverse conditions, and high economic benefits. Seedlings are the foundation of afforestation; planting eucalyptus generally relies on transplanting seedlings, and the quality of these seedlings directly affects the survival rate of afforestation, thus influencing the growth and overall economic benefits of the eucalyptus trees. The root system, as a crucial organ for absorption, synthesis, fixation, and support in plants, plays a vital role in crop growth and development. A robust root system provides sufficient nutrients and water for plant growth, forming the basis for high yields. Plant roots are not only important organs for absorbing nutrients and water but also sites for the assimilation, transformation, and synthesis of many substances, as well as metabolic organs for exchanging substances with the above-ground parts. Their growth and vitality directly affect the overall growth, development, nutritional level, and yield of the plant. The quality of root growth directly impacts the quality of seedlings.
[0004] Currently, there is an urgent need to control the rapid spread of Mikania micrantha. Resource utilization is an excellent approach. This method is safe and efficient, controlling the biosecurity risks of invasive plants while being environmentally friendly, and has significant practical implications. Summary of the Invention
[0005] To achieve the resource utilization of Mikania micrantha, the technical problem to be solved by this invention is to provide a method for promoting the rooting of eucalyptus seedlings using Mikania micrantha aqueous extract, which can significantly increase the root surface area and root diameter of eucalyptus seedlings, effectively increase the total root length, and has no environmental pollution.
[0006] The objective of this invention is achieved through the following technical solution: a method for promoting root growth in eucalyptus seedlings using a water extract of Mikania micrantha, comprising the following steps:
[0007] (1) Dried Mikania micrantha samples: Fresh Mikania micrantha branches and leaves were collected, washed, and dried in an oven at 70°C until constant weight. The dried Mikania micrantha branches and leaves were crushed and passed through a 2mm sieve to obtain Mikania micrantha powder.
[0008] (2) Dissolve Mikania powder: Take 5g of Mikania powder obtained in step (1) and add it to a 1000mL volumetric flask. Then add 500mL of pure water and stir with a glass rod until dissolved. Finally, add pure water and make up to 1000mL.
[0009] (3) Preparation of Mikania micrantha aqueous extract: Place the volumetric flask with the adjusted volume in a shaker and shake for 24 hours to ensure thorough mixing and dissolution. Filter to obtain a Mikania micrantha aqueous extract with a concentration of 5 g / L. Store the prepared Mikania micrantha aqueous extract at 4°C.
[0010] (4) Prepare seedling materials: Select eucalyptus seedlings with similar growth and height, plant them in planting pots of the same size, and fill them with 2L of nutrient soil.
[0011] (5) Apply Mikania micrantha aqueous extract: Take 100 mL of the Mikania micrantha aqueous extract prepared in step (3) and irrigate it onto the eucalyptus seedlings in step (4).
[0012] (6) Subsequent management: The eucalyptus seedlings that were watered with Mikania micrantha extract in step (5) were placed in an outdoor environment for cultivation, and watered once every 2 days.
[0013] Furthermore, the eucalyptus seedlings mentioned in step (4) are healthy eucalyptus seedlings cultivated using conventional methods, and their specifications are preferably 35-40cm in diameter and 2.0-3.0cm in diameter at ground level.
[0014] Furthermore, the preferred size of the cultivation pot in step (4) is 16cm × 16cm (diameter × height), and the volume of the nutrient soil is 2L.
[0015] The present invention has the following advantages and effects compared with the prior art:
[0016] (1) This invention first proposed and proved that the aqueous extract of Mikania micrantha can promote the root growth of eucalyptus seedlings, significantly increase the root surface area and average root diameter, and effectively increase the total root length.
[0017] (2) The raw materials for the water extract of Mikania micrantha are abundant and the preparation method is simple. Compared with traditional eucalyptus fertilizer, it can reduce production costs.
[0018] (3) The water extract of Mikania micrantha uses only Mikania micrantha as raw material and does not add other biochemical reagents. Previous studies have shown that it does not interfere with the green manure effect. It is convenient, simple and safe to use.
[0019] (4) The use of Mikania micrantha water extract does not pollute the environment.
[0020] (5) The method of the present invention also provides a new approach to the study of eucalyptus root growth, which has great practical significance. At the same time, it realizes the utilization of the invasive species Mikania micrantha, opening up a new direction for its future resource development and utilization. Attached Figure Description
[0021] Figure 1 Comparison of eucalyptus seedling root systems after irrigation with 5g / L Mikania micrantha aqueous extract and sterile water. Detailed Implementation
[0022] To make the above-mentioned objects, features, and advantages of the present invention more apparent and understandable, the present invention will be further described in detail below with reference to specific embodiments, but the embodiments of the present invention are not limited thereto. The examples given are only for explaining the present invention and are not intended to limit the scope of the present invention. Unless otherwise specified, the various raw materials and reagents used in the embodiments of the present invention are not limited in source and can all be obtained through commercial purchase.
[0023] The test soil involved in this invention is nutrient soil, which was purchased from the market; the eucalyptus seedlings were purchased from the Guangxi Zhuang Autonomous Region Forestry Research Institute, with a seedling height of 35-40cm and a ground diameter of 2.0-3.0cm; and the Mikania micrantha was taken from the Liuli Branch of the Guangxi State-owned Gaofeng Forest Farm.
[0024] Example 1:
[0025] (1) Collect fresh Mikania micrantha branches and leaves samples, and dry them in an oven at 70℃ until constant weight. After washing, dry the Mikania micrantha branches and leaves, crush them, and pass them through a 2mm sieve to obtain Mikania micrantha powder.
[0026] (2) Take 5g, 10g, 20g and 50g of Mikania powder respectively and add them to a 1000mL volumetric flask. Add 500mL of pure water and stir with a glass rod until dissolved. Then add pure water to the volumetric flask and make up to 1000mL.
[0027] (3) Place the volumetric flask in a shaker and shake for 24 hours to ensure thorough mixing and dissolution. Then filter to obtain Mikania micrantha aqueous extracts with concentrations of 5 g / L, 10 g / L, 20 g / L, and 50 g / L. Store the prepared Mikania micrantha aqueous extracts at 4°C.
[0028] (4) Select 60 eucalyptus seedlings with similar growth and height (35-40cm in height and 2.0-3.0cm in diameter), plant them in 16cm×16cm (diameter×height) plastic flower pots, add 2L of nutrient soil to each pot, keep the soil moist after transplanting, and set aside for later use.
[0029] (5) After the eucalyptus seedlings were well established, watering pots with different concentration gradients of Mikania micrantha extract was applied, with 100 mL applied to each pot. The concentrations of Mikania micrantha extract were 5 g / L, 10 g / L, 20 g / L, and 50 g / L, respectively. 100 mL of sterile water was used as a control for watering. Each treatment was repeated 12 times.
[0030] (6) Eucalyptus seedlings that had been irrigated with Mikania micrantha extract were placed outdoors and labeled with numbers indicating the concentration of the extract and the cultivation date. They were watered every two days. The experiment lasted 45 days.
[0031] (7) After the experiment, the eucalyptus seedlings were harvested whole, rinsed clean with tap water, and the roots were repeatedly rinsed with distilled water and dried with absorbent paper. The total root length, average root diameter and root surface area were measured using a plant root analysis system, calculated using Image-Pro Plus 6.0 software, and the data were statistically analyzed using Microsoft Excel and SPSS 26.0.
[0032] The results of Example 1 are shown in Table 1. Table 1 shows the effects of the five treatments in Example 1 on the root growth of eucalyptus seedlings.
[0033] Table 1. Effects of different treatments on root growth of eucalyptus seedlings
[0034]
[0035] Note: a, b, and c represent the significance level of P at 0.05.
[0036] As shown in Table 1, compared with the control group, irrigating eucalyptus seedlings with aqueous extracts of Mikania micrantha at concentrations of 5 g / L, 10 g / L, 20 g / L, and 50 g / L increased the root surface area, total root length, and average root diameter to varying degrees. Among these, the 5 g / L aqueous extract resulted in the largest root surface area and average root diameter, showing significant differences compared to the control and the other three treatments. Simultaneously, the 50 g / L Mikania micrantha aqueous extract resulted in the largest total root length, but its effect on the total root length was not significantly different from the three aqueous extracts and the control. Considering all factors, the Mikania micrantha aqueous extract significantly promotes root growth in eucalyptus seedlings, with the 5 g / L concentration showing the best effect.
[0037] As shown in the attached figure. Figure 1As shown, the left image shows the rooting of eucalyptus seedlings irrigated with 5g / L Mikania micrantha extract, while the right image shows the rooting of eucalyptus seedlings irrigated with sterile water. The comparison shows that irrigating with Mikania micrantha extract can promote the root growth of eucalyptus seedlings.
[0038] The embodiments described above are merely preferred embodiments of the present invention and are not intended to limit the scope of the present invention. Various modifications and improvements made by those skilled in the art to the technical solutions of the present invention without departing from the spirit of the present invention should fall within the protection scope defined by the claims of the present invention.
Claims
1. A method for promoting rooting of eucalyptus seedlings using a water extract of Mikania micrantha, characterized in that, Using 5g / L of Mikania micrantha aqueous extract can promote the root growth of eucalyptus seedlings, specifically by increasing the root surface area, average root diameter, and total root length. Includes the following steps: (1) Prepare an aqueous extract of Mikania micrantha powder with a concentration of 5 g / L; (2) Irrigate the roots of the eucalyptus seedlings with the aqueous extract of Mikania prepared in step (1); (3) Seedling management: Eucalyptus seedlings that have been irrigated with Mikania micrantha extract are placed in outdoor environments for cultivation, and watered once every 2 days. The aforementioned Mikania micrantha aqueous extract requires the collection of fresh Mikania micrantha branches and leaves, which are washed, dried in an oven at 70°C, pulverized, and then passed through a 2mm sieve. The preparation method of the Mikania micrantha aqueous extract is as follows: according to the required concentration, add 5g of Mikania micrantha powder to a 1000mL volumetric flask, then add 500mL of pure water and stir with a glass rod until dissolved. Finally, add pure water to make up to 1000ml, place in a shaker and shake for 24 hours to fully mix and dissolve. After filtration, it is ready for use.