A fungicidal composition containing clofentezine acetate and thiabendazole

Abstract

A bactericidal composition containing cinnamycin acetate and thiamethoxam, the bactericidal composition comprising cinnamycin acetate, thiamethoxam and a complex synergist. The complex synergist is D-limonene and rhamnose ester, and the mass ratio of D-limonene and rhamnose ester is 4:1 to 1:1. When D-limonene and rhamnose ester are combined in a certain ratio, a synergistic effect is exhibited, and the bactericidal effect of the combined cinnamycin acetate and thiamethoxam on cucumber bacterial angular leaf spot can be significantly improved.

Description

Technical Field

[0001] This invention relates to the field of pesticide technology, and in particular to a fungicidal composition comprising cymoxanil acetate and thiamethoxam. Background Technology

[0002] Bacterial angular leaf spot of cucumber is a bacterial disease caused by *Pseudomonas syringae*, a pathogen of cucumber angular leaf spot. This disease can occur in both seedling and mature plant stages. In seedlings, it primarily affects the cotyledons, while in mature plants, it mainly damages the leaves, petioles, and tendrils. Severe outbreaks can also damage stems and fruits, significantly impacting cucumber yield. Currently, control of cucumber bacterial angular leaf spot mainly relies on chemical agents, such as kasugamycin, streptomycin, kasugamycin-copper hydroxide, and kasugamycin-quinoline copper hydroxide, but their efficacy is not ideal.

[0003] Oxylenol acetate is an alkyl polyamine bactericide. Its bactericidal mechanism is that it can ionize in aqueous solution. Its hydrophilic part contains strong positive charge, which adsorbs various bacteria and viruses that are usually negatively charged, thereby inhibiting the reproduction of bacteria and viruses and coagulating bacterial proteins, thus achieving the best bactericidal effect.

[0004] Thiamethoxam is a novel, broad-spectrum fungicide mainly used to prevent and treat various bacterial and fungal diseases, such as cucumber downy mildew, pear scab, apple scab, citrus anthracnose, and grape black rot. Its fungicidal mechanism primarily involves two aspects: disrupting the nuclear structure of pathogen cells, causing them to lose their vital organs and die; and interfering with the metabolism of pathogen cells, disrupting their physiological processes and ultimately leading to death.

[0005] Existing patent applications (Hailier Pharmaceutical Group Co., Ltd., CN201510733560.2) disclose the use of a combination of thiamethoxam and cymoxanil acetate for the prevention and control of diseases such as bacterial angular leaf spot of cucumber, but the control effect is generally poor and cannot meet the current needs of agricultural production, and needs to be further improved.

[0006] With the increasing use of chemical pesticides, their side effects on the environment and food safety have become a major concern. Reducing the use of chemical pesticides is also an important task that urgently needs to be addressed in pesticide development and application. Summary of the Invention

[0007] The purpose of this invention is to provide a bactericidal composition containing octochlor acetate and thiamethoxam to solve the technical problems of the prior art, which has limited effectiveness in preventing and controlling bacterial angular leaf spot of cucumber and requires large amounts of chemical agents.

[0008] The specific technical solution is as follows:

[0009] A bactericidal composition containing octochlor acetate and thiamethoxam, the bactericidal composition comprising octochlor acetate, thiamethoxam and a compound synergist; the compound synergist being D-limonene and rhamnosyl ester, wherein the mass ratio of D-limonene and rhamnosyl ester is 4:1 to 1:1.

[0010] The bactericidal composition comprises, by weight, 1-10 parts of octochlor acetate, 1-10 parts of thiamethoxam and 1-10 parts of compound synergist.

[0011] The bactericidal composition comprises the following components in weight percentage: 1-10% octochlor acetate, 1-10% thiamethoxam, 1-10% compound synergist, 1-6% dispersant, 1-10% solvent, 1-6% antifreeze, 0.1-2.0% preservative, 0.1-5% thickener, 0.1-0.5% defoamer, and deionized water to make up to 100%.

[0012] Furthermore, the mass ratio of D-limonene to rhamnosyl ester in the compound synergist is 2:1.

[0013] The bactericidal composition is used for the prevention and treatment of bacterial angular leaf spot disease in cucumbers.

[0014] Beneficial technical effects:

[0015] D-Limonene is derived from citrus oil, and rhamnosyl esters are microbial metabolites; both are natural components. When D-limonene and rhamnosyl esters are combined in a certain ratio, they exhibit a synergistic effect, significantly enhancing the fungicidal efficacy of the combined octochlor acetate and thiamethoxam against bacterial angular leaf spot in cucumbers. This significantly reduces the amount of chemically active pesticide ingredients used, improves environmental protection and food safety, and slows down the development of pesticide resistance, making it of significant value for widespread application. Detailed Implementation

[0016] The specific embodiments of the present invention will be described in detail below with reference to the examples, but it should be understood that the scope of protection of the present invention is not limited to the specific embodiments.

[0017] Formulation Examples

[0018] Formulation Example 1

[0019] By weight percentage, octenylamine acetate 1.8%, thiamethoxam 1.2%, fatty alcohol polyoxyethylene ether 2%, cyclohexanone 3%, xanthan gum 3%, ethylene glycol 4%, sodium benzoate 1%, defoamer 0.3%, and deionized water to make up to 100%.

[0020] Formulation Example 2

[0021] By weight percentage, the composition is as follows: 1.8% octochlor acetate, 1.2% thiamethoxam, 3% compound synergist (D-limonene: rhamnosyl ester = 4:1), 2% fatty alcohol polyoxyethylene ether, 3% cyclohexanone, 3% xanthan gum, 4% ethylene glycol, 1% sodium benzoate, 0.3% defoamer, and deionized water to make up to 100%.

[0022] Formulation Example 3

[0023] By weight percentage, 1.8% octochlor acetate, 1.2% thiamethoxam, 3% compound synergist (D-limonene: rhamnosyl ester = 1:1), 2% fatty alcohol polyoxyethylene ether, 3% cyclohexanone, 3% xanthan gum, 4% ethylene glycol, 1% sodium benzoate, 0.3% defoamer, and deionized water to make up to 100%.

[0024] Formulation Example 4

[0025] By weight percentage, 1.8% octochlor acetate, 1.2% thiamethoxam, 3% compound synergist (D-limonene: rhamnosyl ester = 2:1), 2% fatty alcohol polyoxyethylene ether, 3% cyclohexanone, 3% xanthan gum, 4% ethylene glycol, 1% sodium benzoate, 0.3% defoamer, and deionized water to make up to 100%.

[0026] Comparative Example 1

[0027] By weight percentage, octenylamine acetate 1.8%, thiamethoxam 1.2%, D-limonene 3%, fatty alcohol polyoxyethylene ether 2%, cyclohexanone 3%, xanthan gum 3%, ethylene glycol 4%, sodium benzoate 1%, defoamer 0.3%, deionized water to make up 100%.

[0028] Comparative Example 2

[0029] By weight percentage, octenylamine acetate 1.8%, thiamethoxam 1.2%, rhamnosyl ester 3%, fatty alcohol polyoxyethylene ether 2%, cyclohexanone 3%, xanthan gum 3%, ethylene glycol 4%, sodium benzoate 1%, defoamer 0.3%, and deionized water to make up to 100%.

[0030] Comparative Example 3

[0031] By weight percentage, the following ingredients are included: 1.8% octochlor acetate, 1.2% thiamethoxam, 3% synergist (D-limonene: rhamnosyl ester: other synergist chlorogenic acid = 2:1:1), 2% fatty alcohol polyoxyethylene ether, 3% cyclohexanone, 3% xanthan gum, 4% ethylene glycol, 1% sodium benzoate, 0.3% defoamer, and deionized water to make up to 100%.

[0032] Comparative Example 4

[0033] By weight percentage, octenylamine acetate 1.8%, thiamethoxam 1.2%, synergist 3% (D-limonene: rhamnosyl ester = 5:1), fatty alcohol polyoxyethylene ether 2%, cyclohexanone 3%, xanthan gum 3%, ethylene glycol 4%, sodium benzoate 1%, defoamer 0.3%, and deionized water to make up to 100%.

[0034] Comparative Example 5

[0035] By weight percentage, octochlor acetate 1.8%, thiamethoxam 1.2%, synergist 3% (D-limonene: other surfactant sodium dodecyl sulfate = 2:1), fatty alcohol polyoxyethylene ether 2%, cyclohexanone 3%, xanthan gum 3%, ethylene glycol 4%, sodium benzoate 1%, defoamer 0.3%, deionized water to make up to 100%.

[0036] Comparative Example 6

[0037] By weight percentage, octenylamine acetate 1.8%, thiamethoxam 1.2%, synergist 3% (rhamnosyl ester: other synergist chlorogenic acid = 2:1), fatty alcohol polyoxyethylene ether 2%, cyclohexanone 3%, xanthan gum 3%, ethylene glycol 4%, sodium benzoate 1%, defoamer 0.3%, deionized water to make up to 100%.

[0038] Indoor bioassay test

[0039] Test pathogen: Bacterium angularis of cucumber was isolated from diseased leaves in a vegetable greenhouse in Jingyang County, Shaanxi Province. The bacteria were purified by plate culture, and the purified strain was inoculated onto test tube slant culture medium. After being cultured at 28°C for 48 hours, the culture was stored in a refrigerator for later use.

[0040] Experimental steps:

[0041] (1) Preparation of bacterial suspension: The bacterial angular leaf spot pathogen of cucumber was activated on NA / LB solid plates. After incubation at 28℃, single colonies were picked and transferred to NB / LB liquid medium, and then incubated on a shaker at 28℃ and 160r / min for 36h to prepare a bacterial suspension for later use.

[0042] (2) Inoculation and cultivation: Potted cucumber plants that have grown to two leaves and one bud were sprayed with a suspension of cucumber bacterial angular leaf spot pathogen, including a control without the agent. After inoculation, the cucumber seedlings were placed in a humidifier with a relative humidity of 90% and a temperature of 26-28℃ for humidification cultivation.

[0043] (3) Dissolve and dilute the above-mentioned agents with anhydrous ethanol to form a mother liquor, and then use 0.1% Tween 80 aqueous solution to prepare solutions with 5 concentration gradients.

[0044] (4) Chemical treatment: After 24 hours of inoculation and culture, the above-mentioned chemical agent was evenly sprayed onto the cucumber plants. Each treatment was repeated 3 times, with 6 pots per replicate, and a blank control without chemical agent was set up.

[0045] (5) Disease survey: After application of the pesticide, wait until the control group without the pesticide has fully developed the disease, and then calculate the disease index and control effect. Grading is based on the leaf surface area occupied by bacterial angular leaf spot lesions in cucumbers. Grade 0: No lesions; Grade 1: 0%-5%; Grade 3: 5%-25%; Grade 5: 25%-50%; Grade 7: 50%-75%; Grade 9: Over 75%.

[0046] (6) Data Statistical Analysis

[0047] Based on the survey data, the disease index and prevention and control effects of each treatment were calculated.

[0048] Disease index = 100 × ∑(number of diseased leaves at each level × relative level value) / (total number of leaves surveyed × 9)

[0049] Prevention and control effect (%) = 100 × (control disease index - treatment disease index) / control disease index.

[0050] Regression analysis was performed based on the logarithmic values ​​of each pesticide concentration and the corresponding probability values ​​of control efficacy to calculate the EC50 of each pesticide. 50 The synergistic effect ratio is calculated as follows: Synergistic effect ratio = (Inhibition concentration EC of fungicide used alone) 50 / The use of a mixture of bactericide and synergist to inhibit medium concentration EC 50 .

[0051] Table 1. Results of indoor bioassays on the effects of chemical treatments on *Cucumber angular leaf spot*.

[0052]

[0053] Table 1 shows that the compound synergist (D-limonene and rhamnosyl ester) significantly improved the fungicidal effect of the combination of octochlor acetate and thiamethoxam against bacterial angular leaf spot of cucumber. When the ratio of D-limonene to rhamnosyl ester was between 4:1 and 1:1, the synergistic ratio was greater than 1.43, indicating a significant synergistic effect; especially when the ratio was 2:1, the synergistic ratio reached 2.77, showing a remarkable synergistic effect. The synergistic ratios of adding D-limonene and rhamnosyl ester alone were 1.23 and 1.12, respectively. When the ratio was 5:1, the synergistic ratio was 1.20, comparable to that of adding D-limonene and rhamnosyl ester alone. Therefore, the compound synergist exhibits a higher synergistic effect than the individual addition of D-limonene and rhamnosyl ester within a certain ratio range, demonstrating a synergistic effect.

[0054] When the ratio of D-limonene to rhamnosyl ester in the compound synergist is 2:1, the addition of a certain proportion of other synergist chlorogenic acid results in a synergistic ratio of 1.93, which is significantly lower than the synergistic ratio of 2.77 of the compound synergist (D-limonene to rhamnosyl ester in a 2:1 ratio).

[0055] When the ratio of D-limonene to rhamnosyl ester in the compound synergist is 2:1, and rhamnosyl ester is replaced by other surfactants such as sodium dodecyl sulfate, the synergistic ratio is 1.15, which is comparable to the synergistic ratio of adding D-limonene and rhamnosyl ester alone.

[0056] When the ratio of D-limonene to rhamnosyl ester in the compound synergist is 2:1, and D-limonene is replaced by chlorogenic acid, another synergist, the synergistic ratio is 1.18, which is comparable to the synergistic ratio of adding D-limonene and rhamnosyl ester alone.

[0057] In summary, the compound synergist (D-limonene and rhamnosyl ester) exhibits a significant synergistic effect. Simultaneously, it significantly reduces the amount of chemically active ingredients used in pesticides, improves environmental protection and food safety, and slows the development of pesticide resistance in diseases, making it of significant value for widespread application.

[0058] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit it.

Claims

1. A bactericidal composition containing octochlor acetate and thiamethoxam, characterized in that: The bactericidal composition includes octochlor acetate, thiamethoxam and a compound synergist; the compound synergist is D-limonene and rhamnosyl ester, and the mass ratio of D-limonene and rhamnosyl ester is 4:1 to 1:

1.

2. The bactericidal composition according to claim 1, characterized in that: The bactericidal composition comprises, by weight, 1-10 parts of octochlor acetate, 1-10 parts of thiamethoxam and 1-10 parts of compound synergist.

3. The bactericidal composition according to claim 1, characterized in that: The bactericidal composition comprises the following components in weight percentage: 1-10% octochlor acetate, 1-10% thiamethoxam, 1-10% compound synergist, 1-6% dispersant, 1-10% solvent, 1-6% antifreeze, 0.1-2.0% preservative, 0.1-5% thickener, 0.1-0.5% defoamer, and deionized water to make up to 100%.

4. The bactericidal composition according to claim 1, characterized in that: The mass ratio of D-limonene to rhamnosyl ester is 2:

1.

5. Use of the bactericidal composition according to any one of claims 1 to 4 for the prevention and control of bacterial angular leaf spot disease of cucumber.

Images