A method for determining the mating and insemination interval between Yaoshan chicken hens and roosters of different breeds

Abstract

The present invention discloses a method capable of accurately determining the insemination interval between Yaoshan chicken hens and roosters of different breeds. The determination method first uses Yaoshan chicken roosters and Yaoshan chicken hens for pure breeding, and then uses Zhuxiang chicken roosters with large genetic background differences and Yaoshan chicken hens for hybridization after stopping insemination. The proportion of hybrid offspring of Zhuxiang chicken × Yaoshan chicken can be intuitively and simply judged according to the appearance characteristics and genotypes of the chicks. When the proportion of hybrid offspring of Zhuxiang chicken × Yaoshan chicken reaches 100%, it can be determined that the semen of the Zhuxiang chicken rooster has completely replaced the semen of the Yaoshan chicken rooster that was previously residual in the body of the Yaoshan chicken hen, thereby determining the appropriate insemination interval between Yaoshan chicken hens and roosters of different breeds. The method is simple, fast and effective, significantly improving the utilization efficiency of the effective production cycle of Yaoshan chicken breed hens and improving the economic value. It is suitable for promotion and application in the field of poultry breeding technology.

Description

Technical Field

[0001] The invention relates to the technical field of poultry breeding, and in particular to a method for determining the mating and insemination interval between Yaoshan chicken hens and roosters of different breeds. Background Art

[0002] Yaoshan chicken is mainly produced in Yaoshan Yao Township, Libo County, Qiannan Prefecture, Guizhou Province. It is a meat and egg dual-purpose breed that has been domesticated for a long time under the unique breeding customs and management methods of ethnic minorities. It has been listed in the "Chinese Livestock and Poultry Genetic Resources: Poultry". Yaoshan chicken has a red upright single comb, white skin, blue or dark brown shanks, red feathers and black tails for roosters, dark hemp feathers for hens, and light brown eggs. Yaoshan chicken has become one of the main featured poultry breeds in Guizhou Province because of its bright fur color, tolerance to roughage, strong resistance to adversity, and good meat and egg quality. After years of breeding of this breed, the genetics of Yaoshan chicken is stable, but the improvement of production performance has encountered bottlenecks. In order to flexibly respond to diversified market demands and fully develop and utilize the germplasm resources of Yaoshan chicken, during the egg-laying cycle of Yaoshan chicken breed hens, it is often necessary to replace different breeds of breeding roosters through artificial insemination to obtain different hybrid offspring.

[0003] Zhuxiang Chicken is another Guizhou local chicken breed listed in the "Chinese Livestock and Poultry Genetic Resources: Poultry". It is named after its origin, Chishui City, Zunyi City, Guizhou Province, which is known as the "Hometown of Nanzhu". It is a local breed for both meat and eggs. Because the locals prefer black-bone individuals, they are intentionally selected and retained, resulting in the appearance characteristics of black hair, skin, meat, bones, beaks, crowns, and toes. It has the characteristics of fast growth, strong resistance to adversity, tender meat, and green-shelled eggs. It is an extremely precious breeding material, but its development and utilization level is still relatively low.

[0004] There is a special structure at the junction of the uterus and vagina of the hen - the sperm storage gland, which can store the sperm of the rooster and maintain the fertilization ability of the sperm for a period of time, so that the hen can continue to produce fertilized eggs. Even if the semen of another rooster is re-injected, the semen of the previous rooster may not be completely inactivated in the hen's body due to insufficient insemination intervals. The hen may also have a probability of producing offspring with the previous rooster, resulting in the inability to completely obtain 100% of the binary hybrid offspring group, which also causes great interference to targeted breeding. At present, in actual production, Yaoshan chicken hens have the phenomenon of short egg-laying peak duration, which is only 6 to 7 weeks, which is common in most local breeds. Some farms are eager for quick results and start to re-inject semen from new roosters on the 10th day after the Yaoshan chicken hens stop insemination, thereby producing offspring chicks with mixed appearance. If the appearance of the new breed rooster is similar to that of the Yaoshan chicken, it will cause practitioners to make wrong judgments about the genetic characteristics of the Yaoshan chicken, which is not conducive to the orderly advancement of breeding work. In order to ensure the target offspring, Yaoshan chicken hens and Yaoshan chicken roosters are usually stopped from mating for more than 21 days, and then the semen of another rooster is re-injected. Although the accuracy of the breeding target can be guaranteed, the insemination interval is longer than the most suitable insemination interval for Yaoshan chicken hens to mate with new breed roosters and obtain 100% binary hybrid offspring, which causes the Yaoshan chicken breed hens to be idle for a long time, greatly reducing the utilization efficiency of the effective production cycle of Yaoshan chicken breed hens, causing economic losses. How to accurately determine the most suitable insemination interval for Yaoshan chicken hens and Yaoshan chicken roosters to mate with different breeds of roosters after normal mating and obtain 100% binary hybrid offspring is a problem that needs to be solved urgently. Summary of the invention

[0005] The technical problem to be solved by the present invention is to provide a method for determining the mating and insemination interval of Yaoshan chicken hens and Yaoshan chicken roosters with different breeds, which can accurately determine the most suitable insemination interval for Yaoshan chicken hens and Yaoshan chicken roosters to mate with different breeds of roosters after normal mating and obtain 100% binary hybrid offspring.

[0006] The technical solution adopted by the present invention to solve the technical problem is: the method for determining the mating and insemination interval between Yaoshan chicken hens and roosters of different breeds comprises the following steps:

[0007] 1) Father line selection:

[0008] Select existing Yaoshan chicken roosters aged 34 weeks, with a weight standard of 1.8-2.0kg, and appearance standards of red upright single comb, bright red and yellow neck feathers, red back, black abdomen and tail, and green shanks; a single semen collection volume of more than 0.8mL, milky white and thick, pH of 7-8, density of 3-5 billion / mL, and vitality of more than 0.7;

[0009] Select existing 34-week-old Zhuxiang chicken roosters, and select green-shell egg-positive homozygous individuals through molecular identification. The weight standard is 1.9-2.1kg, and the appearance standard is black upright single comb, black feathers, black legs, and black skin; the single semen collection volume is more than 0.8mL, milky white, thick, pH 7-8, density 3-5 billion / mL, and vitality is more than 0.7;

[0010] 2) Maternal selection:

[0011] Select existing Yaoshan chicken hens of 34 weeks old, with a weight standard of 1.5-1.7 kg, a weekly egg production rate of more than 60%, and a cumulative egg production of more than 55 eggs at 34 weeks of age; the appearance standard is dark hemp feathers and green shanks;

[0012] 3) The Yaoshan chicken roosters selected from the paternal line and the Yaoshan chicken hens selected from the maternal line are purebred, and the Yaoshan chicken roosters and Yaoshan chicken hens are artificially inseminated in a certain ratio, repeated once every 4 days, and repeated insemination ≥ 10 times. After the last insemination, the breeding eggs are collected for 2 consecutive days for incubation;

[0013] 4) On the Nth day and the N+2th day after the fertilization of the Yaoshan chicken rooster and the Yaoshan chicken hen was stopped, the Yaoshan chicken hen and the Zhuxiang chicken rooster selected from the paternal line were artificially inseminated twice in a certain ratio, and the breeding eggs of 2 days were continuously collected on the N+3rd and N+4th days for incubation;

[0014] 5) Determine the appearance and genotype of the chicks after hatching the hatching eggs obtained in step 3). If the appearance of the chicks meets the appearance characteristics of Yaoshan chicken × Yaoshan chicken chicks, proceed to step 6); if the appearance of the chicks does not meet the appearance characteristics of Yaoshan chicken × Yaoshan chicken chicks, terminate the determination; the appearance characteristics of Yaoshan chicken × Yaoshan chicken chicks are flower feathers, white skin, green legs, and the genotype is a green shell egg negative homozygous genotype;

[0015] 6) Determine the appearance and genotype of the chicks after hatching the hatching eggs obtained in step 4), and determine the number of chicks that meet the appearance characteristics of Yaoshan chicken × Yaoshan chicken chicks and the number of chicks that meet the appearance characteristics of Zhuxiang chicken × Yaoshan chicken chicks according to the collection time of the hatching eggs, and calculate the proportion of chicks that meet the appearance characteristics of Zhuxiang chicken × Yaoshan chicken chicks; the appearance characteristics of Yaoshan chicken × Yaoshan chicken chicks are flower feathers, white skin, green shanks, and the genotype is a green shell egg negative homozygous genotype; the appearance characteristics of Zhuxiang chicken × Yaoshan chicken chicks are black feathers, black skin, black shanks or green shanks, and the genotype is a green shell egg positive heterozygous genotype;

[0016] 7) Determine the time for collecting breeding eggs corresponding to 100% proportion of chicks that meet the appearance characteristics and genotypes of Zhuxiang chicken × Yaoshan chicken, and then determine the corresponding N value, and determine the minimum N value, which is the most suitable insemination interval for Yaoshan chicken hens and Yaoshan chicken roosters to mate with roosters of different breeds after normal mating and obtain 100% binary hybrid offspring.

[0017] Furthermore, in step 3), artificial insemination is performed on Yaoshan chicken roosters and Yaoshan chicken hens in a ratio of 1:9.

[0018] Furthermore, in step 3), the artificial insemination volume is 30 μL / head, the insemination depth is 1.5 cm, and the insemination time is 15:30-16:30 on the day of insemination.

[0019] Furthermore, in step 4), the Yaoshan chicken hens were artificially inseminated twice with the Zhuxiang chicken roosters selected from the paternal line at a ratio of 9:1.

[0020] Furthermore, in step 4), the artificial insemination volume is 30 μL / head, the insemination depth is 1.5 cm, and the insemination time is 15:30-16:30 on the day of insemination.

[0021] Furthermore, in step 4), N=4, 7, 10, 13, 16, 19, 22, 25.

[0022] The beneficial effects of the present invention are as follows: the method for determining the appropriate insemination interval for mating Yaoshan chicken hens with roosters of different breeds first uses Yaoshan chicken roosters and Yaoshan chicken hens for pure breeding, and then uses Bamboo-xiang chicken roosters with relatively large genetic background differences and Yaoshan chicken hens for hybridization after stopping insemination, and the sperm of the Yaoshan chicken rooster can still survive in the oviduct of the Yaoshan chicken hen for a period of time and maintain fertilization ability, and the semen of the newly-injected Bamboo-xiang chicken rooster will gradually replace the semen of the Yaoshan chicken rooster previously remaining in the body of the Yaoshan chicken hen, and there will be a probability of producing two kinds of offspring, Yaoshan chicken×Yaoshan chicken chicks and Bamboo-xiang chicken×Yaoshan chicken chicks, because Yaoshan chickens do not carry the green-shell egg gene, the appearance characteristics of Yaoshan chicken×Yaoshan chicken chicks are flower feathers, white skin, blue legs, and the genotype is green-shell egg-negative homozygote; the genotype of the Bamboo-xiang chicken rooster is green-shell egg-positive homozygote, and the green-shell egg, black feathers, and black skin are all dominant. The hybrid offspring chicks of Zhuxiang Chicken × Yaoshan Chicken inherit the characteristics of the father Zhuxiang Chicken, showing black feathers, black skin, black or green shanks and green-shelled egg-positive heterozygous genotype, which are significantly different from Yaoshan Chicken × Yaoshan Chicken chicks. Using this method, the proportion of hybrid offspring of Zhuxiang Chicken × Yaoshan Chicken chicks can be intuitively and simply judged according to the appearance characteristics and genotypes of the chicks. When the proportion of hybrid offspring of Zhuxiang Chicken × Yaoshan Chicken chicks reaches 100%, it can be determined that the semen of the Zhuxiang Chicken rooster has completely replaced the semen of the Yaoshan Chicken rooster remaining in the body of the Yaoshan Chicken hen, thereby determining the appropriate insemination interval for mating Yaoshan Chicken hens with roosters of different breeds. The method is simple, fast and effective. Compared with determining whether it is a binary hybrid offspring through paternity testing at the genetic level, its cost is greatly reduced, which reduces the cost of breeding and provides great convenience for breeding work. Through experimental verification, it was found that the appropriate insemination interval for Yaoshan chicken hens to mate with different breeds of roosters is 16 days. Compared with the traditional method of re-injecting semen from a new rooster on the 10th day after the Yaoshan chicken hens stopped insemination, resulting in mixed appearance and blood of offspring chicks, the accuracy of breeding goals has been greatly improved; compared with the traditional method of stopping Yaoshan chicken hens from mating for more than 21 days and then re-injecting semen from another rooster, the utilization efficiency of the effective production cycle of Yaoshan chicken breed hens has been effectively improved, and the economic value has been improved. The present invention has created a binary hybridization model of Zhuxiang chicken and Yaoshan chicken for the first time, and used this model to further determine the appropriate insemination interval for Yaoshan chicken hens to mate with different breeds of roosters, and can also improve the development and utilization of Yaoshan chicken and Zhuxiang chicken, two Guizhou characteristic local breeds. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 This is a schematic diagram of gel electrophoresis during genotype screening of the Zhuxiang chicken rooster of the present invention;

[0024] Figure 2 This is a schematic diagram of the appearance of the offspring of Yaoshan chicken×Yaoshan chicken and Zhuxiang chicken×Yaoshan chicken according to the present invention;

[0025] Figure 3is a schematic diagram of gel electrophoresis during genotype screening of chicks according to the present invention;

[0026] Figure 4 It is a schematic diagram of the proportion of the offspring of Zhuxiang chicken × Yaoshan chicken described in the present invention. DETAILED DESCRIPTION

[0027] like Figure 1-4 As shown, the method for determining the suitable insemination interval for mating Yaoshan chicken hens with roosters of different breeds comprises the following steps:

[0028] 1) Father line selection:

[0029] Select existing Yaoshan chicken roosters aged 34 weeks, with a weight standard of 1.8-2.0kg, and appearance standards of red upright single comb, bright red and yellow neck feathers, red back, black abdomen and tail, and green shanks; a single semen collection volume of more than 0.8mL, milky white and thick, pH of 7-8, density of 3-5 billion / mL, and vitality of more than 0.7; select 27 Yaoshan chicken roosters;

[0030] The existing 34-week-old bamboo chicken roosters were selected, and green-shell egg-positive homozygous genotype individuals were selected by molecular identification. The weight standard was 1.9-2.1 kg, and the appearance standard was jet-black upright single comb, black feathers, black legs, and black skin; the single semen collection volume was more than 0.8 mL, milky white and thick, with a pH of 7-8, a density of 3-5 billion / mL, and a vitality of more than 0.7; the green-shell egg-positive homozygous genotype bamboo chicken roosters were obtained in the following way: according to the characteristic that the green-shell egg trait is autosomal dominant inheritance, molecular marker technology was used to perform group detection on bamboo chicken roosters, such as Figure 1 As shown, in which only one 206bp band appears during gel electrophoresis, it is a positive homozygous genotype; two 206bp and 312bp bands appear, which is a heterozygous genotype, and the Zhuxiang chicken rooster with only one 206bp band of positive homozygous genotype is selected, wherein the molecular marker technology is implemented by the patented technology with application number 201510194180.6, application date 2015-04-22, and patent name "Primer Composition for Identifying the Genotype of Green Shell Laying Hens and Its Application"; 27 Zhuxiang chicken roosters are selected;

[0031] 2) Maternal line selection:

[0032] Select existing Yaoshan chicken hens of 34 weeks old, with a weight standard of 1.5-1.7 kg, a weekly egg production rate of more than 60%, and a cumulative egg production of more than 55 eggs at 34 weeks old; the appearance standard is dark hemp feathers and green shanks; select 243 Yaoshan chicken hens and randomly divide them into 9 groups, with 3 replicates in each group and 9 hens in each replicate;

[0033] 3) The Yaoshan chicken roosters selected from the paternal line and the Yaoshan chicken hens selected from the maternal line were purebred, and the Yaoshan chicken roosters and Yaoshan chicken hens were artificially inseminated at a ratio of 1:9, repeated once every 4 days, the insemination volume was 30μL / hen, the insemination depth was 1.5cm, the insemination time was 15:30-16:30 on the day of insemination, and the insemination was repeated ≥10 times. After the last insemination, the breeding eggs were collected for 2 consecutive days for hatching; the day when the Yaoshan chicken hens stopped inseminating the Yaoshan chicken semen was recorded as d0;

[0034] 4) On the Nth day and the N+2th day after the fertilization of the Yaoshan chicken rooster and the Yaoshan chicken hen was stopped, the Yaoshan chicken hen and the Zhuxiang chicken rooster selected from the paternal line were artificially inseminated twice in a ratio of 9:1, N was the insemination interval, N=4, 7, 10, 13, 16, 19, 22, 25, the insemination volume was 30 μL / hen, the insemination depth was 1.5 cm, the insemination time was 15:30-16:30 on the day of insemination, and the breeding eggs for 2 consecutive days were collected on the N+3rd and N+4th days for incubation;

[0035] 5) Determine the appearance of the chicks after hatching the eggs obtained in step 3), such as Figure 2 , Figure 3 As shown, the Yaoshan chicken × Yaoshan chicken chicks have colorful feathers, white skin, green legs, and a green-shell egg-negative homozygous genotype;

[0036] 6) Determine the appearance of the chicks after hatching the hatching eggs obtained in step 4), and count the number of chicks that meet the appearance characteristics of Yaoshan chicken × Yaoshan chicken chicks and the number of chicks that meet the appearance characteristics of Zhuxiang chicken × Yaoshan chicken chicks according to the time of egg collection, and calculate the proportion of chicks that meet the appearance characteristics of Zhuxiang chicken × Yaoshan chicken chicks; Figure 2 , Figure 3 As shown, the appearance characteristics of Yaoshan chicken × Yaoshan chicken chicks are colorful feathers, white skin, green shanks, and the genotype is a green shell egg negative homozygous genotype; the appearance characteristics of Zhuxiang chicken × Yaoshan chicken chicks are black feathers, black skin, black shanks or green shanks, and the genotype is a green shell egg positive heterozygous genotype; Figure 3 As shown, the heterozygous genotype appears when two bands of 206bp and 312bp appear during gel electrophoresis; the negative homozygous genotype appears when only one band of 312bp appears, and the genotype of the chicks is determined by using the patented technology of application number 201510194180.6, application date 2015-04-22, and patent name "Primer Composition for Identifying the Genotype of Green Shell Laying Hens and Its Application";

[0037] The results of the appearance determination and genotype ratio of the offspring chicks at different insemination intervals are shown in Table 1 and Figure 4 As shown:

[0038] Table 1 Appearance determination results and genotype ratios of offspring chicks at different insemination intervals

[0039]

[0040]

[0041] It can be seen from Table 1 that when N≥16, the proportion of hybrid chickens of Zhuxiang chicken×Yaoshan chicken offspring is 100%, which can determine that the semen of Zhuxiang chicken roosters has completely replaced the semen of Yaoshan chicken roosters that previously remained in the bodies of Yaoshan chicken hens. Therefore, it is concluded that the appropriate insemination interval for Yaoshan chicken hens and Yaoshan chicken roosters to mate with other breeds of roosters after normal mating is at least 16 days, that is, the most suitable insemination interval for Yaoshan chicken hens and Yaoshan chicken roosters to mate with different breeds of roosters after normal mating and obtain 100% binary hybrid offspring is 16 days.

[0042] The method for determining the appropriate insemination interval for mating Yaoshan chicken hens with roosters of different breeds is to first use Yaoshan chicken roosters and Yaoshan chicken hens for pure breeding, and then use Zhuxiang chicken roosters with relatively large genetic background differences to crossbreed Yaoshan chicken hens after stopping insemination. The sperm of the Yaoshan chicken rooster can still survive in the oviduct of the Yaoshan chicken hen for a period of time and maintain fertilization ability. The newly injected semen of the Zhuxiang chicken rooster will gradually replace the semen of the Yaoshan chicken rooster that previously remained in the body of the Yaoshan chicken hen. During this period, there will be a probability of producing two types of offspring, Yaoshan chicken×Yaoshan chicken chicks and Zhuxiang chicken×Yaoshan chicken chicks. Because Yaoshan chickens do not carry the green shell egg gene, the appearance characteristics of Yaoshan chicken×Yaoshan chicken chicks are colorful feathers, white skin, and blue legs, and the genotype is green shell egg negative homozygous; the genotype of the Zhuxiang chicken rooster is green shell egg positive homozygous, and the green shell eggs, black feathers, and black skin are all dominant. The hybrid offspring chicks of Zhuxiang Chicken × Yaoshan Chicken inherit the characteristics of the father Zhuxiang Chicken, showing black feathers, black skin, black or green shanks and green-shelled egg-positive heterozygous genotype, which are significantly different from Yaoshan Chicken × Yaoshan Chicken chicks. Using this method, the proportion of hybrid offspring of Zhuxiang Chicken × Yaoshan Chicken chicks can be intuitively and simply judged according to the appearance characteristics and genotypes of the chicks. When the proportion of hybrid offspring of Zhuxiang Chicken × Yaoshan Chicken chicks reaches 100%, it can be determined that the semen of the Zhuxiang Chicken rooster has completely replaced the previous residual semen of the Yaoshan Chicken rooster, thereby determining the appropriate insemination interval for Yaoshan Chicken hens to mate with roosters of different breeds. The method is simple, fast and effective. Compared with paternity testing at the genetic level to determine whether it is a binary hybrid offspring, its cost is greatly reduced, which reduces the cost of breeding and provides great convenience for breeding work. Empirical experiments have verified that the appropriate insemination interval for Yaoshan chicken hens to mate with different breeds of roosters is 16 days. Compared with the traditional method of re-injecting semen from a new rooster on the 10th day after the Yaoshan chicken hens stopped insemination, resulting in mixed appearance and blood of offspring chicks, the accuracy of breeding goals has been greatly improved; compared with the traditional method of stopping Yaoshan chicken hens from mating for more than 21 days and then re-injecting semen from another rooster, the utilization efficiency of the effective production cycle of Yaoshan chicken breed hens has been effectively improved, and the economic value has been improved. The present invention has created a binary hybridization model of Zhuxiang chicken and Yaoshan chicken for the first time, and used this model to further determine the appropriate insemination interval for Yaoshan chicken hens to mate with different breeds of roosters, and can also improve the development and utilization of Yaoshan chicken and Zhuxiang chicken, two Guizhou characteristic local breeds.

[0043] The above description shows and describes several preferred embodiments of the invention, but as mentioned above, it should be understood that the invention is not limited to the form disclosed herein, and should not be regarded as excluding other embodiments, but can be used in various other combinations, modifications and environments, and can be modified within the scope of the invention concept described herein through the above teachings or the technology or knowledge of the relevant field. Changes and modifications made by those skilled in the art should be within the scope of protection of the claims attached to the invention without departing from the spirit and scope of the invention.

Claims

1. A method for determining the mating and insemination interval between Yaoshan chicken hens and roosters of different breeds, characterized in that: The steps include: 1) Father line selection: Select existing Yaoshan chicken roosters aged 34 weeks, with a weight standard of 1.8-2.0 kg, and appearance standards of red upright single comb, bright red and yellow neck feathers, red back, black abdomen and tail, and green shanks; a single semen collection volume of more than 0.8 mL, milky white and thick, pH of 7-8, density of 3-5 billion / mL, and vitality of more than 0.7; Select existing 34-week-old Zhuxiang chicken roosters, and select green-shell egg-positive homozygous individuals through molecular identification. The weight standard is 1.9-2.1 kg, and the appearance standard is black upright single comb, black feathers, black legs, and black skin; the single semen collection volume is more than 0.8 mL, milky white and thick, pH is 7-8, density is 3-5 billion / mL, and vitality is more than 0.7; 2) Maternal selection: Select existing Yaoshan chicken hens of 34 weeks old, with a weight standard of 1.5-1.7 kg, a weekly egg production rate of more than 60%, and a cumulative egg production of more than 55 eggs at 34 weeks of age; the appearance standard is dark hemp feathers and green shanks; 3) The Yaoshan chicken roosters selected from the paternal line and the Yaoshan chicken hens selected from the maternal line are bred in pure form. The Yaoshan chicken roosters and Yaoshan chicken hens are artificially inseminated in a certain ratio, repeated every 4 days, and repeated insemination ≥ 10 times. After the last insemination, the breeding eggs are collected for 2 consecutive days for incubation; 4) On the Nth day and the N+2th day after the fertilization of Yaoshan chicken roosters and Yaoshan chicken hens stopped, the Yaoshan chicken hens were artificially inseminated twice with the Zhuxiang chicken roosters selected from the paternal line according to a certain ratio, and the breeding eggs of two days were collected and incubated on the N+3rd and N+4th days, N=4, 7, 10, 13, 16, 19, 22, 25; 5) Determine the appearance and genotype of the chicks after hatching the eggs obtained in step 3). If the appearance of the chicks meets the appearance characteristics of Yaoshan chicken × Yaoshan chicken chicks, proceed to step 6); if the appearance of the chicks does not meet the appearance characteristics of Yaoshan chicken × Yaoshan chicken chicks, terminate the determination; the appearance characteristics of Yaoshan chicken × Yaoshan chicken chicks are flower feathers, white skin, green legs, and the genotype is a green shell egg negative homozygous genotype; 6) Determine the appearance and genotype of the chicks hatched from the eggs obtained in step 4), and count the number of chicks that meet the appearance characteristics of Yaoshan chicken × Yaoshan chicken chicks and the number of chicks that meet the appearance characteristics of Zhuxiang chicken × Yaoshan chicken chicks according to the time of egg collection, and calculate the proportion of chicks that meet the appearance characteristics of Zhuxiang chicken × Yaoshan chicken chicks; the appearance characteristics of Yaoshan chicken × Yaoshan chicken chicks are flower feathers, white skin, green shanks, and the genotype is a green shell egg negative homozygous genotype; the appearance characteristics of Zhuxiang chicken × Yaoshan chicken chicks are black feathers, black skin, black shanks or green shanks, and the genotype is a green shell egg positive heterozygous genotype; 7) Determine the time for collecting breeding eggs when the proportion of chicks that meet the appearance characteristics of Zhuxiang chicken × Yaoshan chicken is 100%, and then determine the corresponding N value, and determine the minimum N value, which is the most suitable insemination interval for Yaoshan chicken hens and Yaoshan chicken roosters to mate with roosters of different breeds after normal mating and obtain 100% binary hybrid offspring.

2. The method for determining the mating and insemination interval between Yaoshan chicken hens and roosters of different breeds according to claim 1, characterized in that: In step 3), Yaoshan chicken roosters and Yaoshan chicken hens are artificially inseminated in a ratio of 1:

9.

3. The method for determining the mating and insemination interval between Yaoshan chicken hens and roosters of different breeds according to claim 1, characterized in that: In step 3), the artificial insemination volume is 30 μL / animal, the insemination depth is 1.5 cm, and the insemination time is 15:30-16:30 on the day of insemination.

4. The method for determining the mating and insemination interval between Yaoshan chicken hens and roosters of different breeds according to claim 1, characterized in that: In step 4), the Yaoshan chicken hens were artificially inseminated twice with the Zhuxiang chicken roosters selected from the paternal line at a ratio of 9:

1.

5. The method for determining the mating and insemination interval between Yaoshan chicken hens and roosters of different breeds according to claim 1, characterized in that: In step 4), the artificial insemination volume is 30 μL / animal, the insemination depth is 1.5 cm, and the insemination time is 15:30-16:30 on the day of insemination.

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