Polyether sulfone microspheres, preparation method and application

Abstract

The application discloses a kind of polyether sulfone microspheres, preparation method and application, comprising the following steps: step 1: vinyl imidazole bromide salt, acrylic acid and azobisdimethylvaleronitrile are dissolved in solvent, stirring is carried out under nitrogen protection condition, fully reacts, precipitates, and is filtered to obtain polymer;Step 2: the polymer obtained in step 1 is dissolved in solvent to obtain polymer solution;Step 3: the polymer solution obtained in step 2 and polyether sulfone solution are blended, and polyether sulfone microspheres are obtained by electrospinning;The polyether sulfone microspheres obtained by the application have a diameter of 500-800 μm, have a loose porous structure, have a large specific surface area, and have good bilirubin adsorption effect.

Description

Technical Field

[0001] This invention relates to the field of biomaterials technology, specifically to a polyethersulfone microsphere, its preparation method, and its application. Background Technology

[0002] Liver failure is a severe liver injury caused by multiple factors, leading to serious impairment or decompensation of synthetic, detoxification, metabolic, and biotransformation functions. It presents as a group of clinical syndromes, primarily manifested by jaundice, coagulation dysfunction, hepatorenal syndrome, hepatic encephalopathy, and ascites. According to 2020 statistics, the global prevalence of acute-on-chronic liver failure (ACLF) among patients with decompensated cirrhosis is 35%, with a global 90-day mortality rate of 58%, compared to only 14% for non-ACLF patients. Data from 2019 indicates that there are approximately 7 million cirrhosis patients in my country. Currently, there are no specific drugs or treatments for liver failure in medical practice. The principle of treatment emphasizes early diagnosis and early treatment, implementing appropriate etiological and comprehensive treatment measures, and actively preventing and treating complications. While there are currently no effective medical treatments for liver failure, liver transplantation is an effective treatment for patients with intermediate to advanced liver failure. Liver transplantation has been performed in China for over 40 years, with more than 20,000 surgeries performed. The 1-, 3-, and 5-year survival rates for liver transplant patients are 84%, 75%, and 71%, respectively. However, due to widespread issues with sourcing and affordability, less than 1% of patients have the opportunity to receive a liver transplant. Therefore, artificial liver support systems, which can temporarily replace some functions of the failing liver, create conditions for hepatocyte regeneration and liver function recovery, or allow patients to await a liver transplant, are particularly important.

[0003] Currently, my country primarily employs the Double Plasma Molecular Absorber System (DPMAS) treatment modality, utilizing a combination of two adsorbents: neutral macroporous resin (HA330-Ⅱ) and ion exchange resin (BS330). The resin in the HA330-Ⅱ hemoperfusion unit is a relatively broad-spectrum adsorbent with a macroporous structure and extremely large specific surface area. It adsorbs medium to large molecular toxins, including inflammatory factors, through van der Waals forces and molecular sieve action. The resin in the BS330 bilirubin adsorbent is a specific adsorbent for bilirubin, adsorbing bilirubin, bile acids, and other substances through electrostatic interactions and lipophilic binding specificity. These technologies involve complex treatment modalities, cumbersome procedures, and high costs. Furthermore, they often require the supplementation of blood products such as albumin or fresh plasma. However, blood products are currently scarce in China, and the transfusion process may lead to bloodborne infections, limiting the widespread clinical application of these artificial liver systems. Summary of the Invention

[0004] This invention addresses the problems existing in the prior art by providing a polyethersulfone microsphere that can efficiently adsorb bilirubin, its preparation method, and its application.

[0005] The technical solution adopted in this invention is as follows:

[0006] A method for preparing polyethersulfone microspheres includes the following steps:

[0007] Step 1: Dissolve vinylimidazolium bromide, acrylic acid, and initiator in a solvent to form a mixed solution. Stir under nitrogen protection to allow the reaction to proceed fully. After precipitation and filtration, the polymer is obtained. The molar ratio of vinylimidazolium bromide to acrylic acid is 3–10:1.

[0008] Step 2: Dissolve the polymer obtained in Step 1 in a solvent to obtain a polymer solution;

[0009] Step 3: Blend the polymer solution and polyethersulfone solution obtained in Step 2 to form a blend solution, and obtain polyethersulfone microspheres by electrospinning; wherein the mass ratio of polymer to polyethersulfone is 1 to 4:3.

[0010] Furthermore, in step 1, the reaction temperature is 80°C and the reaction time is 24 hours.

[0011] Furthermore, in step 1, the vinylimidazolium bromide is one of 1-vinyl-3-butylimidazolium bromide, 1-vinyl-3-octylimidazolium bromide, and 1-vinyl-3-benzylimidazolium bromide.

[0012] Furthermore, the initiator in step 1 is azobisisobutyronitrile, and the mass concentration of azobisisobutyronitrile in the mixed solution is 2 wt.%.

[0013] Furthermore, in step 3, the coagulation bath solution for electrospinning is an aqueous ethanol solution; the injection speed of the electrospinning process is 1.5 mm / min, and the voltage is 6V to 8V.

[0014] Furthermore, in step 3, the polymer concentration in the blend solution is 4–16 wt.%, and the polyethersulfone solution concentration is 12 wt.%.

[0015] Furthermore, the polyethersulfone microspheres obtained by electrospinning in step 3 are subjected to deprotonation treatment; the deprotonation treatment process is as follows:

[0016] The obtained polyethersulfone microspheres were soaked in 0.1M sodium hydroxide solution for 24 hours.

[0017] A polyethersulfone microsphere, wherein the polyethersulfone microsphere has a diameter of 500-800 μm and the interior of the microsphere has a loose porous structure; the interior of the microsphere has tubular pores and the walls of the tubular pores have micropores.

[0018] An application of polyethersulfone microspheres, characterized in that the polyethersulfone microspheres are used in the preparation of medical devices.

[0019] Furthermore, the medical device is a device for treating and / or improving hyperbilirubinemia.

[0020] The beneficial effects of this invention are:

[0021] (1) The polyethersulfone microspheres obtained by the present invention have a diameter of 500-800 μm, a loose and porous structure, and a large specific surface area, which can improve the bilirubin adsorption effect.

[0022] (2) The polyethersulfone microspheres obtained in this invention have nitrogen positive ions, which combine with negatively charged bilirubin molecules through electrostatic interaction and have strong π-π conjugation interaction, thereby increasing the adsorption effect of polymer microspheres on bilirubin.

[0023] (3) The substituents of vinylimidazolium bromide in the polyethersulfone microspheres obtained in this invention can form a large number of hydrogen bonds with bilirubin, which can further increase the adsorption effect of the adsorbent on bilirubin. Attached Figure Description

[0024] Figure 1 This is a SEM image of the polyethersulfone microspheres obtained in Example 7 of the present invention.

[0025] Figure 2 This is a schematic diagram of the hemolysis results of the polyethersulfone microspheres obtained in Example 7 of the present invention.

[0026] Figure 3 The results show the statistical results of cell viability after 7 days of co-culturing polyethersulfone microspheres obtained in Example 7 of this invention with cells.

[0027] Figure 4 The results of fluorescence staining of cells after co-culturing polyethersulfone microspheres obtained in Example 7 of this invention with cells for 7 days are shown. Detailed Implementation

[0028] The present invention will be further described below with reference to the accompanying drawings and specific embodiments.

[0029] A method for preparing polyethersulfone microspheres includes the following steps:

[0030] Step 1: Vinylimidazolium bromide, acrylic acid, and the initiator azobisisobutyronitrile are dissolved in N,N-dimethylformamide to obtain a mixed solution. The solution is stirred under nitrogen purging at room temperature for 30 min, then reacted under vacuum and nitrogen protection at 80°C for 24 h. The reaction solution is precipitated with acetone, filtered, and dried. The vinylimidazolium bromide in Step 1 is one of 1-vinyl-3-butylimidazolium bromide, 1-vinyl-3-octylimidazolium bromide, or 1-vinyl-3-benzylimidazolium bromide.

[0031] The molar ratio of vinylimidazolium bromide to acrylic acid is 3–10:1; the mass concentration of azobisisobutyronitrile in the mixed solution is 2 wt.%.

[0032] Step 2: Dissolve the polymer (imidazolium quaternary ammonium salt polymer) obtained in Step 1 in the solvent N,N-dimethylformamide to obtain a polymer solution;

[0033] Step 3: Blend the polymer solution obtained in Step 2 with the polyethersulfone solution to obtain a blended solution. The mass concentration of the polymer in the blended solution is 4-16 wt.% and the mass concentration of the polyethersulfone solution is 12 wt.%.

[0034] Polyethersulfone microspheres were obtained by electrospinning; the coagulation bath solution for electrospinning was an aqueous ethanol solution; the injection speed during the electrospinning process was 1.5 mm / min, and the voltage was 6V to 8V. The mass ratio of polymer to polyethersulfone was 1 to 4:3.

[0035] The polyethersulfone microspheres obtained by electrospinning were deprotonated. The deprotonation process is as follows:

[0036] The obtained polyethersulfone microspheres were soaked in 0.1M sodium hydroxide solution for 24 hours.

[0037] A polyethersulfone microsphere, wherein the polyethersulfone microsphere has a diameter of 500-800 μm and the interior of the microsphere has a loose porous structure; the interior of the microsphere has tubular pores and the walls of the tubular pores have micropores.

[0038] An application of polyethersulfone microspheres in the manufacture of medical devices. These medical devices are for the treatment and / or improvement of hyperbilirubinemia. The polyethersulfone microspheres are used as bilirubin adsorbents in hemoperfusion devices.

[0039] Example 1

[0040] A method for preparing polyethersulfone microspheres includes the following steps:

[0041] Step 1: Dissolve 20 mmol of 1-vinyl-3-butylimidazolium bromide, 4 mmol of acrylic acid, and azobisisobutyronitrile in 20 mL of N,N-dimethylformamide to form a mixed solution with a mass concentration of 2 wt.% for azobisisobutyronitrile. Purge with nitrogen and stir for 30 min at room temperature, then react under vacuum and nitrogen protection at 80 °C for 24 h. Precipitate the reaction solution with acetone, filter, and dry at 80 °C to obtain the polymer.

[0042] Step 2: Dissolve the polymer obtained in Step 1 in the solvent N,N-dimethylformamide to obtain a polymer solution.

[0043] Step 3: Blend the polymer solution and polyethersulfone solution obtained in Step 2 to form a blend solution; wherein the mass concentration of polyethersulfone in the blend solution is 12 wt.% and the mass concentration of polymer is 4 wt.%.

[0044] Polyethersulfone microspheres were obtained by electrospinning; the coagulation bath solution for electrospinning was a 15% (v / v) aqueous ethanol solution; the injection speed during the electrospinning process was 1.5 mm / min, and the voltage was 6 V. Microspheres with a diameter of 500-800 μm were obtained, and after being immersed in 0.1 M sodium hydroxide solution for 24 hours for deprotonation, the desired polyethersulfone microspheres were obtained.

[0045] Example 2

[0046] A method for preparing polyethersulfone microspheres includes the following steps:

[0047] Step 1: Dissolve 20 mmol of 1-vinyl-3-butylimidazolium bromide, 4 mmol of acrylic acid, and azobisisobutyronitrile in 20 mL of N,N-dimethylformamide to form a mixed solution with a mass concentration of 2 wt.% of azobisisobutyronitrile. Purge with nitrogen and stir for 30 min at room temperature, then react under vacuum and nitrogen protection at 80 °C for 24 h. Precipitate the reaction solution with acetone, filter, and dry at 80 °C to obtain the polymer.

[0048] Step 2: Dissolve the polymer obtained in Step 1 in the solvent N,N-dimethylformamide to obtain a polymer solution.

[0049] Step 3: Blend the polymer solution and polyethersulfone solution obtained in Step 2 to form a blend solution; wherein the mass concentration of polyethersulfone in the blend solution is 12 wt.% and the mass concentration of polymer is 8 wt.%.

[0050] Polyethersulfone microspheres were obtained by electrospinning; the coagulation bath solution for electrospinning was a 15% (v / v) aqueous ethanol solution; the injection speed during the electrospinning process was 1.5 mm / min, and the voltage was 6 V. Microspheres with a diameter of 500-800 μm were obtained, and after being immersed in 0.1 M sodium hydroxide solution for 24 hours for deprotonation, the desired polyethersulfone microspheres were obtained.

[0051] Example 3

[0052] A method for preparing polyethersulfone microspheres includes the following steps:

[0053] Step 1: Dissolve 20 mmol of 1-vinyl-3-butylimidazolium bromide, 4 mmol of acrylic acid, and azobisisobutyronitrile in 20 mL of N,N-dimethylformamide to form a mixed solution with a mass concentration of 2 wt.% of azobisisobutyronitrile. Purge with nitrogen and stir for 30 min at room temperature, then react under vacuum and nitrogen protection at 80 °C for 24 h. Precipitate the reaction solution with acetone, filter, and dry at 80 °C to obtain the polymer.

[0054] Step 2: Dissolve the polymer obtained in Step 1 in the solvent N,N-dimethylformamide to obtain a polymer solution.

[0055] Step 3: Blend the polymer solution and polyethersulfone solution obtained in Step 2 to form a blend solution; wherein the mass concentration of polyethersulfone in the blend solution is 12 wt.% and the mass concentration of polymer is 12 wt.%.

[0056] Polyethersulfone microspheres were obtained by electrospinning; the coagulation bath solution for electrospinning was a 15% (v / v) aqueous ethanol solution; the injection speed during the electrospinning process was 1.5 mm / min, and the voltage was 8 V. Microspheres with a diameter of 500-800 μm were obtained, and after being immersed in 0.1 M sodium hydroxide solution for 24 hours for deprotonation, the desired polyethersulfone microspheres were obtained.

[0057] Example 4

[0058] A method for preparing polyethersulfone microspheres includes the following steps:

[0059] Step 1: Dissolve 20 mmol of 1-vinyl-3-butylimidazolium bromide, 4 mmol of acrylic acid, and azobisisobutyronitrile in 20 mL of N,N-dimethylformamide to form a mixed solution with a mass concentration of 2 wt.% of azobisisobutyronitrile. Purge with nitrogen and stir for 30 min at room temperature, then react under vacuum and nitrogen protection at 80 °C for 24 h. Precipitate the reaction solution with acetone, filter, and dry at 80 °C to obtain the polymer.

[0060] Step 2: Dissolve the polymer obtained in Step 1 in the solvent N,N-dimethylformamide to obtain a polymer solution.

[0061] Step 3: Blend the polymer solution and polyethersulfone solution obtained in Step 2 to form a blend solution; wherein the mass concentration of polyethersulfone in the blend solution is 12 wt.% and the mass concentration of polymer is 16 wt.%.

[0062] Polyethersulfone microspheres were obtained by electrospinning; the coagulation bath solution for electrospinning was a 15% (v / v) aqueous ethanol solution; the injection speed during the electrospinning process was 1.5 mm / min, and the voltage was 7.5 V. Microspheres with a diameter of 500-800 μm were obtained, and after being immersed in 0.1 M sodium hydroxide solution for 24 hours for deprotonation, the desired polyethersulfone microspheres were obtained.

[0063] Example 5

[0064] A method for preparing polyethersulfone microspheres includes the following steps:

[0065] Step 1: Dissolve 20 mmol of 1-vinyl-3-butylimidazolium bromide, 2 mmol of acrylic acid, and azobisisobutyronitrile in 20 mL of N,N-dimethylformamide to form a mixed solution with a mass concentration of 2 wt.% of azobisisobutyronitrile. Purge with nitrogen and stir for 30 min at room temperature, then react under vacuum and nitrogen protection at 80 °C for 24 h. Precipitate the reaction solution with acetone, filter, and dry at 80 °C to obtain the polymer.

[0066] Step 2: Dissolve the polymer obtained in Step 1 in the solvent N,N-dimethylformamide to obtain a polymer solution.

[0067] Step 3: Blend the polymer solution and polyethersulfone solution obtained in Step 2 to form a blend solution; wherein the mass concentration of polyethersulfone in the blend solution is 12 wt.% and the mass concentration of polymer is 8 wt.%.

[0068] Polyethersulfone microspheres were obtained by electrospinning; the coagulation bath solution for electrospinning was a 15% (v / v) aqueous ethanol solution; the injection speed during the electrospinning process was 1.5 mm / min, and the voltage was 6 V. Microspheres with a diameter of 500-800 μm were obtained, and after being immersed in 0.1 M sodium hydroxide solution for 24 hours for deprotonation, the desired polyethersulfone microspheres were obtained.

[0069] Example 6

[0070] A method for preparing polyethersulfone microspheres includes the following steps:

[0071] Step 1: Dissolve 30 mmol of 1-vinyl-3-butylimidazolium bromide, 2 mmol of acrylic acid, and azobisisobutyronitrile in 20 mL of N,N-dimethylformamide to form a mixed solution with a mass concentration of 2 wt.% of azobisisobutyronitrile. Purge with nitrogen and stir for 30 min at room temperature, then react under vacuum and nitrogen protection at 80 °C for 24 h. Precipitate the reaction solution with acetone, filter, and dry at 80 °C to obtain the polymer.

[0072] Step 2: Dissolve the polymer obtained in Step 1 in the solvent N,N-dimethylformamide to obtain a polymer solution.

[0073] Step 3: Blend the polymer solution and polyethersulfone solution obtained in Step 2 to form a blend solution; wherein the mass concentration of polyethersulfone in the blend solution is 12 wt.% and the mass concentration of polymer is 8 wt.%.

[0074] Polyethersulfone microspheres were obtained by electrospinning; the coagulation bath solution for electrospinning was a 15% (v / v) aqueous ethanol solution; the injection speed during the electrospinning process was 1.5 mm / min, and the voltage was 6 V. Microspheres with a diameter of 500-800 μm were obtained, and after being immersed in 0.1 M sodium hydroxide solution for 24 hours for deprotonation, the desired polyethersulfone microspheres were obtained.

[0075] Example 7

[0076] A method for preparing polyethersulfone microspheres includes the following steps:

[0077] Step 1: Dissolve 20 mmol of 1-vinyl-3-octylimidazolium bromide, 4 mmol of acrylic acid, and azobisisobutyronitrile in 20 mL of N,N-dimethylformamide to form a mixed solution with a mass concentration of 2 wt.% of azobisisobutyronitrile. Purge with nitrogen and stir for 30 min at room temperature, then react under vacuum and nitrogen protection at 80 °C for 24 h. Precipitate the reaction solution with acetone, filter, and dry at 80 °C to obtain the polymer.

[0078] Step 2: Dissolve the polymer obtained in Step 1 in the solvent N,N-dimethylformamide to obtain a polymer solution.

[0079] Step 3: Blend the polymer solution and polyethersulfone solution obtained in Step 2 to form a blend solution; wherein the mass concentration of polyethersulfone in the blend solution is 12 wt.% and the mass concentration of polymer is 8 wt.%.

[0080] Polyethersulfone microspheres were obtained by electrospinning; the coagulation bath solution for electrospinning was a 15% (v / v) aqueous ethanol solution; the injection speed during the electrospinning process was 1.5 mm / min, and the voltage was 7.5 V. Microspheres with a diameter of 500-800 μm were obtained, and after being immersed in 0.1 M sodium hydroxide solution for 24 hours for deprotonation, the desired polyethersulfone microspheres were obtained.

[0081] Figure 1 The image shows an SEM image of the polyethersulfone microspheres obtained in this embodiment. As can be seen from the image, the polyethersulfone microspheres have a diameter of about 500 μm and a loose porous structure inside, which can increase the specific surface area of ​​the microspheres and improve the adsorption effect.

[0082] Figure 2 The hemolysis results of the polyethersulfone microspheres obtained in this embodiment show that the hemolysis results are similar to those of PES (microspheres obtained by electrospinning only polyethersulfone, i.e., polyethersulfone microspheres in the prior art), indicating that it has good biocompatibility.

[0083] Figure 3 The figure shows the cell viability results after co-culturing the polyethersulfone microspheres obtained in this embodiment with cells for 7 days. The comparative example is PBS solution, and p(VI-8-AA) is the polyethersulfone microsphere obtained in this embodiment. As can be seen from the figure, it has no obvious toxicity to cells and is similar to PBS solution, indicating that the obtained polyethersulfone microspheres have good biocompatibility.

[0084] Figure 4 The results of cell viability fluorescence staining after 7 days of co-culturing polyethersulfone microspheres with cells obtained in this embodiment are shown. Figure 3 The statistical results are consistent.

[0085] Example 8

[0086] A method for preparing polyethersulfone microspheres includes the following steps:

[0087] Step 1: Dissolve 20 mmol of 1-vinyl-3-benzylimidazolium bromide, 4 mmol of acrylic acid, and azobisisobutyronitrile in 20 mL of N,N-dimethylformamide to form a mixed solution with a mass concentration of 2 wt.% of azobisisobutyronitrile. Purge with nitrogen and stir for 30 min at room temperature, then react under vacuum and nitrogen protection at 80 °C for 24 h. Precipitate the reaction solution with acetone, filter, and dry at 80 °C to obtain the polymer.

[0088] Step 2: Dissolve the polymer obtained in Step 1 in the solvent N,N-dimethylformamide to obtain a polymer solution with a mass concentration of 8 wt.%.

[0089] Step 3: Blend the polymer solution obtained in Step 2 with an equal volume of a polyethersulfone solution with a mass concentration of 12 wt.%.

[0090] Polyethersulfone microspheres were obtained by electrospinning; the coagulation bath solution for electrospinning was a 15% (v / v) aqueous ethanol solution; the injection speed during the electrospinning process was 1.5 mm / min, and the voltage was 6 V. Microspheres with a diameter of 500-800 μm were obtained, and after being immersed in 0.1 M sodium hydroxide solution for 24 hours for deprotonation, the desired polyethersulfone microspheres were obtained.

[0091] The bilirubin adsorption rate of the polyethersulfone microspheres obtained in the above examples and the control group polyethersulfone microspheres (obtained by electrospinning directly from polyethersulfone solution) was tested, and the results are shown in Table 1.

[0092] The adsorption rate test process is as follows:

[0093] First, 100 mg of each sample from each example and control group was taken and added to 4 mL of PBS solution with a bilirubin concentration of 200 mg / L. 4 mL of PBS solution with a bilirubin concentration of 200 mg / L without adding the sample was taken as the blank group. After shaking at 37°C for 3 hours, the OD438 nm of each sample was measured using an ELISA reader, and the adsorption rate was calculated.

[0094] Table 1. Results of bilirubin adsorption rate test in Examples 1-8 and the control group

[0095]

[0096] The polyethersulfone microspheres obtained in this invention, after undergoing a substitution reaction, acquire nitrogen ions, which can combine with negatively charged bilirubin molecules through electrostatic interactions and exhibit strong π-π conjugated interactions, thereby increasing the adsorption efficiency of the polymer microspheres for bilirubin. The substituents of vinylimidazolium bromide form numerous hydrogen bonds with bilirubin, further enhancing the adsorption effect of the adsorbent. The polyethersulfone microspheres exhibit selectivity; substituents with carbon chains of different lengths combine with bilirubin molecules at different angles, reducing the adsorption of other normal blood components during the clearance process. The presence of carboxyl groups in the polyethersulfone microspheres improves their blood compatibility and reduces protein adhesion during adsorption.

[0097] Meanwhile, the polyethersulfone microspheres have a diameter of 500–800 μm and a loose, porous internal structure; the microspheres contain tubular pores with micropores on their walls. This porous structure increases the specific surface area of ​​the microspheres, thus enhancing the adsorption effect.

Claims

1. A method for preparing polyethersulfone microspheres, characterized in that, Includes the following steps: Step 1: Dissolve vinylimidazolium bromide, acrylic acid, and initiator in a solvent to form a mixed solution. Stir under nitrogen protection to allow the reaction to proceed fully. After precipitation and filtration, obtain the polymer. The molar ratio of vinylimidazolium bromide to acrylic acid is 3–10:

1. Step 2: Dissolve the polymer obtained in Step 1 in a solvent to obtain a polymer solution; Step 3: Blend the polymer solution and polyethersulfone solution obtained in Step 2 to form a blend solution, and obtain polyethersulfone microspheres by electrospinning; wherein the mass ratio of polymer to polyethersulfone is 1 to 4:

3.

2. The method for preparing polyethersulfone microspheres according to claim 1, characterized in that, In step 1, the reaction temperature is 80 °C and the reaction time is 24 h.

3. The method for preparing polyethersulfone microspheres according to claim 1, characterized in that, In step 1, the vinylimidazolium bromide is one of 1-vinyl-3-butylimidazolium bromide, 1-vinyl-3-octylimidazolium bromide, or 1-vinyl-3-benzylimidazolium bromide.

4. The method for preparing polyethersulfone microspheres according to claim 1, characterized in that, The initiator in step 1 is azobisisobutyronitrile, and the mass concentration of azobisisobutyronitrile in the mixed solution is 2 wt.%.

5. The method for preparing polyethersulfone microspheres according to claim 1, characterized in that, In step 3, the coagulation bath solution for electrospinning is an aqueous ethanol solution; the injection speed of the electrospinning process is 1.5 mm / min, and the voltage is 6 V to 8 V.

6. The method for preparing polyethersulfone microspheres according to claim 1, characterized in that, The polymer concentration in the blend solution formed in step 3 is 4–16 wt.%, and the polyethersulfone solution concentration is 12 wt.%.

7. The method for preparing polyethersulfone microspheres according to claim 1, characterized in that, The polyethersulfone microspheres obtained by electrospinning in step 3 undergo deprotonation treatment; the deprotonation treatment process is as follows: The obtained polyethersulfone microspheres were soaked in 0.1 M sodium hydroxide solution for 24 h.

8. The polyethersulfone microspheres obtained by any one of the preparation methods described in claims 1 to 7, characterized in that, The polyethersulfone microspheres have a diameter of 500–800 μm and a loose, porous internal structure; the microspheres have tubular pores inside, and the walls of the tubular pores have micropores.

9. The application of the polyethersulfone microspheres as described in claim 8, characterized in that, Application of the polyethersulfone microspheres in the preparation of medical devices.

10. The application of the polyethersulfone microspheres according to claim 9, characterized in that, The medical device is a device for treating and / or improving hyperbilirubinemia.

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