A compound long-lasting deodorant and its application

Abstract

This invention discloses a compound long-lasting deodorant and its application. The deodorant comprises a compound solution of plant extracts and a compound solution of microorganisms. The compound solution of plant extracts includes plant extracts, plant essential oils, surfactants, organic solvents, and water. The plant extracts include one or a combination of two of the following: monk fruit leaf extract and black tiger leaf extract. The compound solution of microorganisms includes Pichia pastoris, plant essential oils, and corresponding culture media. The compound solution of plant extracts and the compound solution of microorganisms are used sequentially or in combination. This application provides a deodorant that is both environmentally friendly and achieves both high deodorization efficiency and long-lasting effect. The deodorant of this invention can achieve a removal rate of over 98% for odors. Furthermore, because the bacterial strains can further reproduce and grow in an environment containing organic matter, the deodorizing effect is maintained for a long time.

Description

Technical Field

[0001] This application belongs to the field of chemistry, specifically providing a compound long-lasting deodorant and its application. Background Technology

[0002] Odors are always present in daily life and production. These foul-smelling gases are produced by bacteria decomposing organic matter. 75% to 80% of household waste is organic matter, mainly consisting of fruit peels, vegetable leaves and stalks, leftover food, poultry, animal and fish skin, hair, viscera, fat, feces, offal / blood, leaves, waste paper, flowers, and animal carcasses, along with a certain amount of moisture. During natural digestion, aerobic / anaerobic fermentation produces foul odors, especially in hot weather when fermentation accelerates and the odor becomes more severe. In addition, livestock farms, bio-fertilizer plants, urban sewage treatment plants, septic tanks, and public restrooms also generate large amounts of odors, affecting human health. These sources of odor not only harm the workers involved but also disrupt the lives of nearby residents. The chemical components in these odors include inorganic substances such as hydrogen sulfide and ammonia, as well as volatile organic compounds. These foul-smelling components have a significant adverse impact on human health and the environment.

[0003] The national industry standard CJT 516-2017, "Technical Requirements for Deodorizers for Municipal Solid Waste," states that ammonia and hydrogen sulfide are the most important markers of malodorous gases. Ammonia is a highly irritating gas. Low concentrations of ammonia can irritate the eyes, nose, and respiratory tract mucous membranes, causing burning eyes, nausea, coughing, and sore throat; higher concentrations can cause serious damage to the respiratory and endocrine systems. Hydrogen sulfide is another malodorous and highly toxic substance. Low concentrations of hydrogen sulfide can harm the respiratory system, while higher concentrations (greater than 250 ppm) can lead to death. Given continuous urbanization, the intensification of animal husbandry, and the ever-growing industrial and service activities, the treatment of malodorous gases is essential.

[0004] Currently, the deodorizers sold on the market mainly fall into the following categories: physical deodorizers, chemical deodorizers, microbial deodorizers, and plant-based deodorizers.

[0005] Physical deodorizers deodorize through physical methods and can be divided into masking and adsorption types. Masking deodorizers are mainly products with fragrances and flavorings as their main ingredients. Their mechanism of action is to mask the odor of foul gases with the fragrance. However, their drawback is that they do not fundamentally remove the odor molecules, and inhaling too much may be harmful to human health. Adsorption deodorizers are mainly products with a loose and porous structure. Their mechanism of action is to absorb odor gas molecules through their porous structure.

[0006] Chemical deodorizers are a mixture of various chemically synthesized substances. Their mechanism of action is to deodorize by reacting the chemically synthesized substances with malodor molecules. The disadvantage is that they are harmful to both the human body and the environment.

[0007] Microbial deodorizers contain microorganisms that have deodorizing functions. Their mechanism of action is achieved through the ammonia assimilation, nitrification, and biochemical processes of microorganisms, or by converting organic sulfur odorous substances into sulfates through desulfurization and other processes. However, they are slow to take effect and have high costs.

[0008] Plant-based deodorizers are refined from natural bactericidal and deodorizing factors found in plants. Their deodorizing mechanism involves the active ingredients in the plants reacting with volatile odor molecules through addition, oxidation-reduction, neutralization, and condensation reactions. This alters the structure of the odor molecules, transforming them into non-toxic and odorless substances, resulting in low irritation, non-toxicity, and high deodorizing efficiency. Plant-based deodorizers offer significant advantages in being natural, green, and low-carbon, representing a future direction for deodorizing products. However, their primary principle remains chemical reaction-based deodorization, making them still a consumable deodorizer with insufficient long-lasting effectiveness. Summary of the Invention

[0009] To address the aforementioned problems, the inventors of this application have researched various plant extracts and deodorizing microorganisms. The aim is to prepare a plant extract-based deodorant using a combination of these substances.

[0010] However, considering that plant extracts generally need to remove odors through antibacterial action or reaction with ammonia-containing substances, they lack long-lasting effectiveness. Therefore, the inventors combined microbial-based deodorants with plant-based deodorants in their research and development. A key challenge in this development was that chemical deodorants or plant extracts often possess antibacterial properties, which in turn inhibit microbial growth. The inventors screened various plant extracts and essential oils, identifying those that both deodorized and did not inhibit microbial growth. Simultaneously, by controlling the pH value, they ensured that microbial growth was not inhibited.

[0011] During their research on plant extracts, the inventors noticed that monk fruit leaves (confirmed through chemical testing) contain flavonoids, amino acids, and tea polyphenols. Representative compounds include kaempferol-3-O-α-L-rhamnoside, ferulic acid, emodin, and quercetin, which can react with ammonia or amino-containing compounds. The chemical components in black tiger leaves mainly include sugars, total protein, flavonoids, organic acids, phenols, and triterpenes. Ultraviolet-spectrophotometry analysis revealed the following main chemical components: total sugars 1.12%, total protein 0.40%, polyphenols 1.81%, flavonoids 1.79%, triterpenes 7.65%, and lignans 8.81%. Both can react with ammonia or amino-containing compounds, thus deodorizing.

[0012] Gas chromatography-mass spectrometry analysis showed that rosemary essential oil is mainly composed of terpenoids, including 1,8-cineole (20.21%–29.84%), α-pinene (5.42%–15.79%), camphene (4.18%–5.87%), β-pinene (2.88%–4.8%), camphor (3.65%–16.62%), and bornyl acetate (3.57%–8.94%). The main components of cedarwood essential oil are α-cedrene, β-cedrene, α-atlanticone, γ-cedrene, β-atlanticone, and α-cedrene, both of which have the function of masking odors.

[0013] These substances not only have good deodorizing effects and can be used alone, but their deodorizing principle mainly relies on reaction with ammonia-like substances. Therefore, they can coexist with microbial deodorizers.

[0014] Specifically, on the one hand, the present invention provides a compound long-acting deodorant, which includes: a compound liquid of plant extracts and a compound liquid of microorganisms. The compound liquid of plant extracts includes: plant extracts, plant essential oils, surfactants, organic solvents and water. The plant extracts include one or a combination of two of the following: monk fruit leaf extract and black tiger leaf extract. The compound liquid of microorganisms includes: Pichia pastoris and the corresponding culture medium. The compound liquid of plant extracts and the compound liquid of microorganisms are used sequentially or in combination.

[0015] In one implementation, the plant extract compound solution and the microbial compound solution are used together, and the pH value of the culture medium in the microbial compound solution is adjusted to be less than 5.

[0016] In one implementation, the plant essential oil in the plant extract compound liquid includes at least one of rosemary essential oil and cypress essential oil, and the organic solvent is ethanol.

[0017] In one implementation, the surfactant is Tween 80 and sodium lauryl ether sulfate.

[0018] In one implementation, the plant extract complex liquid contains, by volume, 3% to 8% of the plant extract, 1% to 5% of rosemary essential oil, 1% to 5% of cypress essential oil, 1% to 5% of ethanol, 2% to 4% of surfactant, and 75% to 92% of water.

[0019] In one implementation, the plant extract compound liquid contains, by volume, 3% rosemary essential oil, 3% cypress essential oil, 4% monk fruit leaf extract, 4% black tiger leaf extract, 2% ethanol, 3% surfactant, and 81% water.

[0020] In one implementation, the plant extract compound liquid contains, by volume, 3% rosemary essential oil, 3% cypress essential oil, 3% monk fruit leaf extract, 3% black tiger leaf extract, 2% ethanol, 3% surfactant, and 83% water.

[0021] In one implementation, the microbial composite liquid contains an odor-degrading strain with accession number CCTCC NO: M2020285, and a viable count of 1.5-2.5 OD. 560 .

[0022] On the other hand, the present invention provides a method for preparing a deodorant, the method comprising: extracting an extract from monk fruit leaves / black tiger leaves, and preparing a compound liquid of plant extract components in the following proportions: the content of the plant extract is 3% to 8%, the content of rosemary essential oil is 1% to 5%, the content of cypress essential oil is 1% to 5%, the content of ethanol is 1% to 5%, the content of surfactant is 2% to 4%, and the content of water is 81% to 92%; and cultivating a sterilizing solution using Pichia pastoris strain, adding Pichia pastoris strain (preservation number: CCTCC NO: M2020285) to the fermentation broth (culture medium), allowing the strain to multiply, and testing the strain quantity. When the strain content exceeds 3.0 (OD... 560 Stop culturing when the time is right. The fermentation broth can be made of glucose aqueous solution (6-30 g / L) with 20%-30% by mass of ammonium sulfate added (this mass percentage is the mass percentage of ammonium sulfate relative to glucose).

[0023] In one specific embodiment, the preparation process of the plant extract includes: water extraction of monk fruit leaves / black tiger leaves, concentration of the water extract under reduced pressure, microfiltration using a ceramic membrane, passing the filtrate through a macroporous resin, eluting with 30-50% ethanol, further concentration, filtration of the concentrate using a nanofiltration membrane, and retaining substances with a molecular weight of less than 1000, which are monk fruit leaf extract and black tiger leaf extract.

[0024] In one specific embodiment, the rosemary essential oil and cypress essential oil are obtained by steam distillation. The addition of plant extracts to the rosemary and cypress essential oils enhances their deodorizing effect.

[0025] Another aspect of the present invention provides the application of the aforementioned deodorizing composition in landfills, waste transfer stations, public restrooms, urban septic tanks, polluted water bodies, organic fertilizer plants, and livestock farms.

[0026] The beneficial effects of this application are:

[0027] This application provides an environmentally friendly deodorizer that also offers high deodorization efficiency and long-lasting effectiveness. The deodorizer of this invention can achieve a removal rate of over 97% for odors. Furthermore, because the bacterial strain can further multiply and grow in environments containing organic matter, it maintains long-lasting deodorization. The deodorizer of this invention is particularly suitable for landfills, waste transfer stations, public restrooms, urban septic tanks, polluted water bodies, organic fertilizer plants, or livestock farms. Attached Figure Description

[0028] Figure 1 This is a schematic diagram of the deodorant testing equipment used in the embodiments of the present invention;

[0029] Figure 2 This is a physical image of a deodorization effect testing device used in an embodiment of the present invention;

[0030] Figure 3 This is a picture of a fermenter used to cultivate microbial strains; Figures 4-6 The image shows the results after 24 hours of growth when Pichia pastoris and its culture medium were used in a symbiotic experiment with plant extracts containing rosemary and cypress essential oils. Figures 7-9 The image shows the results after 48 hours of growth when Pichia pastoris and its culture medium were used in a symbiotic experiment with plant extracts containing rosemary and cypress essential oils.

[0031] Figures 10-12 The image shows the results after 24 hours in a symbiotic experiment involving Pichia pastoris and its culture medium with a plant extract containing peppermint essential oil. Figures 13-15 The image shows the results after 48 hours when Pichia pastoris and its culture medium were used in a symbiotic experiment with plant extracts containing peppermint essential oil. Detailed Implementation

[0032] The present invention will be described in detail below with reference to the accompanying drawings and embodiments, but the scope of protection of the present invention is not limited to the scope described in the embodiments.

[0033] Example 1

[0034] The present invention will be further described below with reference to the embodiments. However, the embodiments of the present invention are merely illustrative examples and should not be construed as limiting the present invention under any circumstances.

[0035] Unless otherwise specified, all reagents used in this application are commercially available.

[0036] Monk fruit leaf extract / black tiger leaf extract are available commercially or can be prepared at home.

[0037] The Pichia kudriavzevii OHCC-1 deodorizing strain was collected by our company in the field using PDA medium through steps such as strain culture, strain classification, strain purification, and strain domestication. It is deposited at the China Center for Type Culture Collection (CCTCCC), CCTCC accession number: M2020285, dated July 6, 2020, at Wuhan University, China.

[0038] Detailed preparation process of monk fruit leaf extract / black tiger leaf extract: monk fruit leaves / black tiger leaves are extracted with water, concentrated under reduced pressure, microfiltered with a ceramic membrane, the filtrate is passed through a macroporous resin, then eluted with 30-50% ethanol, concentrated again, and the concentrate is filtered with a nanofiltration membrane. The substance with a molecular weight of less than 1000 is the monk fruit leaf extract / black tiger leaf extract.

[0039] Rosemary essential oil and cypress essential oil are commercially available or can be prepared at home through steam distillation.

[0040] The testing process for the deodorant in this invention will be described below.

[0041] Ammonia removal efficiency test: Use a syringe to draw 5 mL of gas from above the ammonia solution surface and inject it into a gas collection bag; then use a gas sampling pump to draw 5 L of air into the gas collection bag, and detect it using an ammonia detector. Adjust the ammonia concentration to within 70-99 ppm (the specific concentration depends on actual measurement), record it as the initial ammonia concentration. Place the sample solution into a bubble absorption tube and press... Figure 1-2 As shown, a gas collection bag, gas sampling pump, bubble absorption tube, and ammonia detector are connected with rubber tubing to form an ammonia removal performance testing device. The ammonia detector and gas sampling pump are started (flow rate set to 0.5 L / min). -1 Preliminary experiments revealed that the detector reading for ammonia stabilized at 60 seconds and beyond. Therefore, it was necessary to record the readings at 15, 30, and 60 seconds after the gas sampling pump was turned on, repeating the experiment three times and taking the average clearance rate. The clearance rate of ammonia by 10 mL of deionized water was used as a negative control. The clearance rate of ammonia by the test solution is shown in the formula below:

[0042] Ammonia removal rate % =

[0043] In the formula:

[0044] C0 represents the initial concentration of ammonia (ppm).

[0045] C1 represents the ammonia concentration (ppm) after passing through the sample solution for 15s, 30s, and 60s.

[0046] Hydrogen sulfide removal efficiency test: Take 7.5g of ferrous sulfide solid particles (appropriately crushed to increase the reaction rate) into a 1000mL suction flask, add 100g of 9.8% sulfuric acid aqueous solution (take 9.8g of concentrated sulfuric acid, diluted with water to 100g), react for 5min, then use a syringe to draw 5mL of synthesized hydrogen sulfide gas and inject it into a gas collection bag; then use a gas sampling pump to draw 5L of air into the gas collection bag, and detect it with a hydrogen sulfide detector. Adjust the hydrogen sulfide concentration to within 70-99 ppm (the specific concentration is subject to actual measurement), and record it as the hydrogen sulfide concentration before treatment. Take 10mL of sample solution and place it in a 10mL bubble absorption tube, according to... Figure 1-2 Set up a hydrogen sulfide performance testing device. Start the hydrogen sulfide detector and gas sampling pump (flow rate 0.5 L·min). -1 Preliminary experiments revealed that the detector reading for ammonia stabilized after 60 seconds. Therefore, readings were recorded at 15, 30, and 60 seconds after the gas sampling pump was turned on. The experiment was repeated three times, and the average clearance rate was taken. The clearance rate of hydrogen sulfide in a 10 mL sample solution was measured, with an equal volume of deionized water as a negative control. The hydrogen sulfide clearance rate was calculated using the following formula:

[0047] Hydrogen sulfide removal rate % =

[0048] In the formula:

[0049] C0 represents the initial concentration of ammonia (ppm).

[0050] C1 represents the hydrogen sulfide concentration (ppm) after passing through the sample solution for 15s, 30s, and 60s.

[0051] Plant extract complex liquids were tested and experimented on separately. The content of each component in the following formula is by volume.

[0052] Experiment 1

[0053] Preparation method: 1% rosemary essential oil, 1% cypress essential oil, 2% ethanol, 1.5% Tween 80, 1.5% sodium lauryl ether sulfate, and 93% water.

[0054] The removal rate of ammonia (hydrogen sulfide) by 10 mL of sample solution was determined. The deodorization effect was determined by the ammonia (hydrogen sulfide) removal efficiency test. The results are shown in Table 1.

[0055] Experiment 2

[0056] Preparation method: 2% rosemary essential oil, 1% cypress essential oil, 2% ethanol, 1.5% Tween 80, 1.5% sodium lauryl ether sulfate, 91% water.

[0057] The removal rate of ammonia (hydrogen sulfide) by 10 mL of sample solution was determined. The deodorization effect was determined by the ammonia (hydrogen sulfide) removal efficiency test. The results are shown in Table 1.

[0058] Experiment 3

[0059] Preparation method: 3% rosemary essential oil, 3% cypress essential oil, 2% ethanol, 1.5% Tween 80, 1.5% sodium lauryl ether sulfate, 89% water.

[0060] The removal rate of ammonia (hydrogen sulfide) by 10 mL sample solution was determined. The deodorization effect was determined by the ammonia (hydrogen sulfide) removal efficiency test. The results are shown in Table 1.

[0061] Experiment 4

[0062] Preparation method: 4% rosemary essential oil, 4% cypress essential oil, 2% ethanol, 1.5% Tween 80, 1.5% sodium lauryl ether sulfate, 87% water.

[0063] The removal rate of ammonia (hydrogen sulfide) by 10 mL sample solution was determined. The deodorization effect was determined by the ammonia (hydrogen sulfide) removal efficiency test. The results are shown in Table 1.

[0064] Experiment 5

[0065] Preparation method: 5% rosemary essential oil, 5% cypress essential oil, 2% ethanol, 1.5% Tween 80, 1.5% sodium lauryl ether sulfate, 85% water.

[0066] The removal rate of ammonia (hydrogen sulfide) by 10 mL sample solution was determined. The deodorization effect was determined by the ammonia (hydrogen sulfide) removal efficiency test. The results are shown in Table 1.

[0067] Experiment 6

[0068] Preparation method: 3% rosemary essential oil, 3% cypress essential oil, 1% monk fruit leaf extract, 2% ethanol, 1.5% Tween 80, 1.5% sodium lauryl ether sulfate, 88% water.

[0069] The removal rate of ammonia (hydrogen sulfide) by 10 mL sample solution was determined. The deodorization effect was determined by the ammonia (hydrogen sulfide) removal efficiency test. The results are shown in Table 1.

[0070] Experiment 7

[0071] Preparation method: Rosemary essential oil 3%, cypress essential oil 3%, black tiger leaf extract 1%, ethanol 2%, Tween 80 1.5%, sodium lauryl ether sulfate 1.5%, water: 88%.

[0072] The removal rate of ammonia (hydrogen sulfide) by 10 mL sample solution was determined. The deodorization effect was determined by the ammonia (hydrogen sulfide) removal efficiency test. The results are shown in Table 1.

[0073] Experiment 8

[0074] Preparation method: 3% rosemary essential oil, 3% cypress essential oil, 1% monk fruit leaf extract, 1% black tiger leaf extract, 2% ethanol, 1.5% Tween 80, 1.5% sodium lauryl ether sulfate, 87% water.

[0075] The removal rate of ammonia (hydrogen sulfide) by 10 mL sample solution was determined. The deodorization effect was determined by the ammonia (hydrogen sulfide) removal efficiency test. The results are shown in Table 1.

[0076] Experiment 9

[0077] Preparation method: 3% rosemary essential oil, 3% cypress essential oil, 2% monk fruit leaf extract, 2% black tiger leaf extract, 2% ethanol, 1.5% Tween 80, 1.5% sodium lauryl ether sulfate, 85% water.

[0078] The removal rate of ammonia (hydrogen sulfide) by 10 mL sample solution was determined. The deodorization effect was determined by the ammonia (hydrogen sulfide) removal efficiency test. The results are shown in Table 1.

[0079] Experiment 10

[0080] Preparation method: 3% rosemary essential oil, 3% cypress essential oil, 3% monk fruit leaf extract, 3% black tiger leaf extract, 2% ethanol, 1.5% Tween 80, 1.5% sodium lauryl ether sulfate, 83% water.

[0081] The removal rate of ammonia (hydrogen sulfide) by 10 mL sample solution was determined. The deodorization effect was determined by the ammonia (hydrogen sulfide) removal efficiency test. The results are shown in Table 1.

[0082] Experiment 11

[0083] Preparation method: 3% rosemary essential oil, 3% cypress essential oil, 4% monk fruit leaf extract, 4% black tiger leaf extract, 2% ethanol, 1.5% Tween 80, 1.5% sodium lauryl ether sulfate, 81% water.

[0084] The removal rate of ammonia (hydrogen sulfide) by 10 mL sample solution was determined. The deodorization effect was determined by the ammonia (hydrogen sulfide) removal efficiency test. The results are shown in Table 1.

[0085] Experiment 12

[0086] Preparation method: 3% rosemary essential oil, 3% cypress essential oil, 5% monk fruit leaf extract, 5% black tiger leaf extract, 2% ethanol, 1.5% Tween 80, 1.5% sodium lauryl ether sulfate, 79% water.

[0087] The removal rate of ammonia (hydrogen sulfide) by 10 mL sample solution was determined. The deodorization effect was determined by the ammonia (hydrogen sulfide) removal efficiency test. The results are shown in Table 1.

[0088] Table 1. Removal of ammonia (hydrogen sulfide) by 10 mL sample solution

[0089]

[0090] Based on the results in Table 1, the average ammonia removal rates in experiments 1-5 show that higher concentrations of rosemary and cypress essential oils are not necessarily better. The optimal range for adding rosemary and cypress essential oils is 1%–5%, with the highest ammonia removal rate achieved at 3% each. Experiments 6 and 7 show that the deodorant solutions containing monk fruit leaf extract and black tiger leaf extract exhibit better average ammonia removal rates than those containing only rosemary and cypress essential oils. Furthermore, in experiment 8, the combination of monk fruit leaf extract and black tiger leaf extract showed a synergistic effect, enhancing the deodorizing effect. Comparative experiments 8-12 also indicate that higher concentrations of the combined monk fruit leaf extract and black tiger leaf extract are not always better. The optimal concentrations for the combined extracts are 3%–5%, with the best results achieved at 4% each.

[0091] Similarly, according to the results in Table 1, the average hydrogen sulfide removal rates in experiments 1-5 show that higher concentrations of rosemary and cypress essential oils are not necessarily better. The optimal range for adding rosemary and cypress essential oils is 1%–5%, with the highest hydrogen sulfide removal rate achieved at a concentration of 3% each. Experiments 6 and 7 show that the deodorant solutions with added monk fruit leaf extract and black tiger leaf extract exhibit better average hydrogen sulfide removal rates than those containing only rosemary and cypress essential oils. Furthermore, experiments 9-12 demonstrate that the combination of monk fruit leaf extract and black tiger leaf extract produces a synergistic effect, enhancing the deodorizing efficacy. Moreover, when combining monk fruit leaf extract and black tiger leaf extract, higher concentrations are not always better. The optimal concentrations for the combination are 2%–4%, with the best results achieved at 3% each.

[0092] In the compound test, the dosage of the plant extract compound solution that yielded the best effect was selected.

[0093] Experiment 13

[0094] Preparation method: 3% rosemary essential oil, 3% cypress essential oil, 4% monk fruit leaf extract, 4% black tiger leaf extract, 2% ethanol, 1.5% Tween 80, 1.5% sodium lauryl ether sulfate, 81% water.

[0095] Preparation method of microbial compound solution: Prepare a slightly acidic culture medium containing a nitrogen source (ammonia sulfate, amino acids, etc.) and a carbon source (glucose, etc.) in the fermenter. After sterilization and cooling, inoculate with 1% liquid inoculum to cultivate the strain. After cultivation, subsequent bacterial solutions are taken from the concentration adjustment solution of the initial culture. A picture of the fermenter is shown below. Figure 3 As shown.

[0096] The concentration of the bacterial culture medium was adjusted by adding culture medium, rosemary essential oil, or cypress essential oil to achieve a viable cell count of approximately 1.0 (OD). 560 ), and use it as a microbial complex liquid.

[0097] The removal rates of ammonia (hydrogen sulfide) by 5 mL of plant extract compound solution and 5 mL of microbial compound solution were determined. The deodorization effect was determined by the ammonia (hydrogen sulfide) removal efficiency test. The results are shown in Table 2.

[0098] In this test, two bubble receiving tubes were used. 5 mL of plant extract compound solution was added to the first bubble receiving tube, and 5 mL of microbial compound solution was added to the second bubble receiving tube. The length of the vent tube in the two bubble receiving tubes extending below the liquid surface was controlled to be half that of the first group of experiments, so as to ensure that the test conditions in the two rounds were roughly the same, that is, the deodorizing liquid path of the bubble was roughly the same.

[0099] Experiment 14

[0100] Preparation method: 3% rosemary essential oil, 3% cypress essential oil, 4% monk fruit leaf extract, 4% black tiger leaf extract, 2% ethanol, 1.5% Tween 80, 1.5% sodium lauryl ether sulfate, 81% water.

[0101] Microbial complex solution: Weigh 5 mL to achieve a viable bacterial count of approximately 2.0 (OD). 560 The microbial composite solution was used as the test liquid.

[0102] The removal rates of ammonia (hydrogen sulfide) by 5 mL of plant extract compound solution and 5 mL of microbial compound solution were determined. The deodorization effect was determined by the ammonia (hydrogen sulfide) removal efficiency test. The results are shown in Table 2.

[0103] Experiment 15

[0104] Preparation method: 3% rosemary essential oil, 3% cypress essential oil, 4% monk fruit leaf extract, 4% black tiger leaf extract, 2% ethanol, 1.5% Tween 80, 1.5% sodium lauryl ether sulfate, 81% water.

[0105] Microbial complex solution: Weigh 5 mL to achieve a viable bacterial count of approximately 2.5 (OD). 560 The microbial composite solution was used as the test liquid.

[0106] The removal rates of ammonia (hydrogen sulfide) by 5 mL of plant extract compound solution and 5 mL of microbial compound solution were determined. The deodorization effect was determined by the ammonia (hydrogen sulfide) removal efficiency test. The results are shown in Table 2.

[0107] Experiment 16

[0108] Preparation method: 3% rosemary essential oil, 3% cypress essential oil, 4% monk fruit leaf extract, 4% black tiger leaf extract, 2% ethanol, 1.5% Tween 80, 1.5% sodium lauryl ether sulfate, 81% water.

[0109] Microbial complex solution: Weigh 5 mL to achieve a viable bacterial count of approximately 3.0 (OD). 560 The microbial composite solution was used as the test liquid.

[0110] The removal rates of ammonia (hydrogen sulfide) by 5 mL of plant extract compound solution and 5 mL of microbial compound solution were determined. The deodorization effect was determined by the ammonia (hydrogen sulfide) removal efficiency test. The results are shown in Table 2.

[0111]

[0112] Since the deodorizing effect of plant extracts combined with fragrance is already very high, the data shows that using a combination of the two deodorizers does not significantly improve the effect of rapid deodorization in ventilation systems.

[0113] Long-term effectiveness test

[0114] Experiment 17

[0115] Preparation method: 3% rosemary essential oil, 3% cypress essential oil, 4% monk fruit leaf extract, 4% black tiger leaf extract, 2% ethanol, 1.5% Tween 80, 1.5% sodium lauryl ether sulfate, 81% water.

[0116] Microbial complex solution: Weigh 5 mL to achieve a viable bacterial count of approximately 1.0 (OD). 560 The microbial composite solution was used as the test liquid.

[0117] The removal effects of 5 mL of plant extract compound solution and 5 mL of microbial compound solution on excess ammonia (hydrogen sulfide) were determined.

[0118] During the test, ammonia and hydrogen sulfide were continuously introduced into the liquid to be tested until the content of ammonia and hydrogen sulfide no longer decreased. After the test liquid was left to stand at room temperature for 24 hours, ammonia was introduced into the bubble receiving tube again and the test was carried out. The results are shown in Table 3.

[0119] Experiment 18

[0120] Preparation method: 3% rosemary essential oil, 3% cypress essential oil, 4% monk fruit leaf extract, 4% black tiger leaf extract, 2% ethanol, 1.5% Tween 80, 1.5% sodium lauryl ether sulfate, 81% water.

[0121] Microbial complex solution: Weigh 5 mL to achieve a viable bacterial count of approximately 1.5 (OD). 560 The microbial composite solution was used as the test liquid.

[0122] The removal effects of 5 mL of plant extract compound solution and 5 mL of microbial compound solution on excess ammonia (hydrogen sulfide) were determined.

[0123] During the test, ammonia and hydrogen sulfide were continuously introduced into the liquid to be tested until the content of ammonia and hydrogen sulfide no longer decreased. After the test liquid was left to stand at room temperature for 24 hours, ammonia and hydrogen sulfide were introduced into the bubble receiving tube again and the test was carried out. The results are shown in Table 3.

[0124] Experiment 19

[0125] Preparation method: 3% rosemary essential oil, 3% cypress essential oil, 4% monk fruit leaf extract, 4% black tiger leaf extract, 2% ethanol, 1.5% Tween 80, 1.5% sodium lauryl ether sulfate, 81% water.

[0126] Microbial complex solution: Weigh 5 mL to achieve a viable bacterial count of approximately 2.0 (OD). 560 The microbial composite solution was used as the test liquid.

[0127] The removal effects of 5 mL of plant extract compound solution and 5 mL of microbial compound solution on excess ammonia (hydrogen sulfide) were determined.

[0128] During the test, ammonia and hydrogen sulfide were continuously introduced into the liquid to be tested until the content of ammonia and hydrogen sulfide no longer decreased. After the test liquid was left to stand at room temperature for 24 hours, ammonia was introduced into the bubble receiving tube again and the test was carried out. The results are shown in Table 3.

[0129] Experiment 20

[0130] Preparation method: 3% rosemary essential oil, 3% cypress essential oil, 4% monk fruit leaf extract, 4% black tiger leaf extract, 2% ethanol, 1.5% Tween 80, 1.5% sodium lauryl ether sulfate, 81% water.

[0131] The test results were determined when 10 mL of plant extract compound solution was used to remove excess ammonia (hydrogen sulfide) and then ammonia was introduced a second time.

[0132] During the test, ammonia and hydrogen sulfide were continuously introduced into the liquid to be tested until the content of ammonia and hydrogen sulfide no longer decreased. After the test liquid was left to stand at room temperature for 24 hours, ammonia was introduced into the bubble receiving tube again and the test was carried out. The results are shown in Table 3.

[0133] Experiment 21

[0134] Microbial complex solution: Weigh 10 mL to achieve a viable bacterial count of approximately 2.0 (OD). 560 The microbial composite solution was used as the test liquid.

[0135] During the test, ammonia and hydrogen sulfide were continuously introduced into the liquid to be tested until the content of ammonia and hydrogen sulfide no longer decreased. After the test liquid was left to stand at room temperature for 24 hours, ammonia was introduced into the bubble receiving tube again and the test was carried out. The results are shown in Table 3.

[0136] Table 3 Long-term test results

[0137]

[0138] Based on this data, the combination of deodorizing bacteria and two deodorizing agents significantly improves the effect of secondary deodorization after supersaturation compared to using only plant extract compound solution, with a significantly higher viable bacterial count (OD). 560 A value of 2 can achieve a good long-lasting deodorizing effect. However, in general, the sterilization efficiency decreases to some extent after supersaturation.

[0139] Mixed testing

[0140] Experiment 22

[0141] Preparation method: 3% rosemary essential oil, 3% cypress essential oil, 4% monk fruit leaf extract, 4% black tiger leaf extract, 2% ethanol, 1.5% Tween 80, 1.5% sodium lauryl ether sulfate, 81% water.

[0142] Microbial complex solution: Weigh 5 mL to achieve a viable bacterial count of approximately 2.0 (OD). 560 The microbial composite solution was used as the test liquid.

[0143] The solution was mixed with 5 mL of plant extract compound solution and 5 mL of microbial compound solution. Acetic acid or a small amount of dilute hydrochloric acid was added to adjust the pH value of the solution to 3, and a scavenging test was performed. The test results are shown in Table 4.

[0144] Experiment 23

[0145] Preparation method: 3% rosemary essential oil, 3% cypress essential oil, 4% monk fruit leaf extract, 4% black tiger leaf extract, 2% ethanol, 1.5% Tween 80, 1.5% sodium lauryl ether sulfate, 81% water.

[0146] Microbial complex solution: Weigh 5 mL to achieve a viable bacterial count of approximately 2.0 (OD). 560 The microbial composite solution was used as the test liquid.

[0147] The solution was mixed with 5 mL of plant extract compound solution and 5 mL of microbial compound solution, and the pH value of the solution was adjusted to 5. The scavenging test was then performed, and the test results are shown in Table 4.

[0148] Experiment 24

[0149] Preparation method: 3% rosemary essential oil, 3% cypress essential oil, 4% monk fruit leaf extract, 4% black tiger leaf extract, 2% ethanol, 1.5% Tween 80, 1.5% sodium lauryl ether sulfate, 81% water.

[0150] Microbial complex solution: Weigh 5 mL to achieve a viable bacterial count of approximately 2.0 (OD). 560 The microbial composite solution was used as the test liquid.

[0151] The solution was mixed with 5 mL of plant extract compound solution and 5 mL of microbial compound solution, and the pH value of the solution was adjusted to 7. The scavenging test was then performed, and the test results are shown in Table 4.

[0152] Experiment 25

[0153] Preparation method: 3% rosemary essential oil, 3% cypress essential oil, 4% monk fruit leaf extract, 4% black tiger leaf extract, 2% ethanol, 1.5% Tween 80, 1.5% sodium lauryl ether sulfate, 81% water.

[0154] Microbial complex solution: Weigh 5 mL to achieve a viable bacterial count of approximately 2.0 (OD). 560 The microbial composite solution was used as the test liquid.

[0155] The solution was mixed with 5 mL of plant extract compound solution and 5 mL of microbial compound solution, and the pH value of the solution was adjusted to 9. The scavenging test was then performed, and the test results are shown in Table 4.

[0156] Experiment 26

[0157] Preparation method: 3% rosemary essential oil, 3% cypress essential oil, 4% monk fruit leaf extract, 4% black tiger leaf extract, 2% ethanol, 1.5% Tween 80, 1.5% sodium lauryl ether sulfate, 81% water.

[0158] Microbial complex solution: Weigh 5 mL to achieve a viable bacterial count of approximately 2.0 (OD). 560 The microbial composite solution was used as the test liquid.

[0159] The solution was mixed with 5 mL of plant extract compound solution and 5 mL of microbial compound solution, and the pH value of the solution was adjusted to 11. The scavenging test was then performed, and the test results are shown in Table 4.

[0160] Experiment 27

[0161] Preparation method: 3% tea tree oil, 3% peppermint oil, 4% monk fruit leaf extract, 4% black tiger leaf extract, 2% ethanol, 1.5% Tween 80, 1.5% sodium lauryl ether sulfate, 81% water.

[0162] Microbial complex solution: Weigh 5 mL to achieve a viable bacterial count of approximately 2.0 (OD). 560 The microbial composite solution was used as the test liquid.

[0163] The solution was mixed with 5 mL of plant extract compound solution and 5 mL of microbial compound solution, and the pH value of the solution was adjusted to 5. The scavenging test was then performed, and the test results are shown in Table 4.

[0164] Experiment 28

[0165] Preparation method: 3% rosemary essential oil, 3% cypress essential oil, 8% yucca extract, 2% ethanol, 1.5% Tween 80, 1.5% sodium lauryl ether sulfate, 81% water.

[0166] Microbial complex solution: Weigh 5 mL to achieve a viable bacterial count of approximately 2.0 (OD). 560 The microbial composite solution was used as the test liquid.

[0167] The solution was mixed with 5 mL of plant extract compound solution and 5 mL of microbial compound solution, and the pH value of the solution was adjusted to 5. The scavenging test was then performed, and the test results are shown in Table 4.

[0168] Table 4 Results of Mixed Tests

[0169]

[0170] The data shows that in an acidic environment with a pH of 3-5, the combination of these two ingredients can effectively remove odors. However, when the pH rises to neutral or even alkaline, the odor removal rate drops significantly. Therefore, the deodorizing composition formed by combining the strain of this invention with monk fruit leaf extract and black tiger leaf extract can only be used alone or in combination under acidic conditions; it cannot be used under alkaline conditions.

[0171] Furthermore, even under the same acidic conditions, the deodorization rate decreased slightly when the essential oil was replaced with a combination of tea tree oil and peppermint oil. The decrease was even greater when the deodorizing plant extract was replaced with yucca extract, indicating poor coexistence with microorganisms and the inability to exert a synergistic effect.

[0172] To further verify the symbiotic relationship between Pichia pastoris and essential oils in this invention, a symbiotic experiment was conducted.

[0173] like Figures 4-6 The image shows the results after 24 hours of a symbiotic experiment involving a culture medium containing Pichia pastoris strain and plant extracts (monk fruit leaf extract and black tiger leaf extract) containing 3% rosemary essential oil and 3% cypress essential oil. Figures 4-6 The percentages of each numbered microbial culture medium are as follows: No. 1 (5%), No. 2 (10%), No. 3 (20%). Here, 20% refers to the content of the culture medium containing the bacterial strain (bacterial concentration 1.6 billion / mL, cultured to the highest concentration using yeast enrichment medium). Figures 7-9 The image shows the results after 48 hours when a culture medium containing Pichia pastoris strain was used in a symbiotic experiment with plant extracts containing rosemary and cypress essential oils.

[0174] like Figures 10-12The image shows the results after 24 hours when a culture medium containing Pichia pastoris was used in a symbiotic experiment with a plant extract containing 6% peppermint oil. Figures 10-12 The percentages of microbial culture medium in the medium are as follows: Figure 10 (5%) Figure 11 (10%) Figure 12 (20%). For example... Figures 13-15 The image shows the results after 48 hours when a culture medium containing Pichia pastoris was used in a symbiotic experiment with a plant extract containing peppermint essential oil.

[0175] contrast Figure 4-9 as well as Figures 10-15 It is evident that when plant extracts containing rosemary and cypress essential oils were used in the symbiotic experiment with Pichia pastoris culture, the bacterial strains grew and reproduced very well, with a uniform and dense distribution of microbial concentration and high activity. However, the symbiotic experiment with peppermint essential oil and Pichia pastoris culture was less successful. After 24 hours of standing, sedimentation occurred, and the microbial concentration distribution was uneven. After 48 hours, although the bacterial strains also grew and reproduced, the amount of reproduction was visibly less than that of the group containing rosemary and cypress essential oils.

[0176] In summary, this invention provides a deodorant that can be prepared based on pure natural plant extracts. By combining the water extract of monk fruit leaves / black tiger leaves with essential oils whose main component is terpenoids, this invention can achieve high deodorization efficiency. Furthermore, by combining the deodorizing components based on plant extracts with the deodorizing components based on microorganisms, a longer-lasting deodorizing effect can be achieved.

[0177] Although the principles of the present invention have been described in detail above with reference to preferred embodiments, those skilled in the art should understand that the above embodiments are merely illustrative explanations of the implementation of the present invention and are not intended to limit the scope of the present invention. The details in the embodiments do not constitute a limitation on the scope of the present invention. Any obvious changes, such as equivalent transformations or simple substitutions, based on the technical solutions of the present invention without departing from the spirit and scope of the present invention fall within the protection scope of the present invention.

Claims

1. A compound long-lasting deodorant, comprising: The compound solution comprises a plant extract and a microbial compound solution. The plant extract compound solution includes plant extracts, plant essential oils, surfactants, organic solvents, and water. The plant extracts include one or a combination of two of the following: monk fruit leaf extract and black tiger leaf extract. The microbial compound solution includes a Pichia pastoris deodorizing strain and a corresponding culture medium. The preservation number of the Pichia pastoris deodorizing strain is CCTCC NO: M2020285. The plant extract compound solution and the microbial compound solution are used together to adjust the pH value of the culture medium in the microbial compound solution to be less than 5. The plant essential oils in the plant extract compound solution include rosemary essential oil and cypress essential oil. The organic solvent is ethanol, and the surfactants are Tween 80 and sodium lauryl ether sulfate.

2. The deodorant with compound long-lasting effect according to claim 1, characterized in that, The volume ratio of the plant extract compound solution and the microbial compound solution is between 1:1 and 6:

1.

3. The deodorant with compound long-lasting effect according to claim 2, characterized in that, The volume ratio of the plant extract compound solution and the microbial compound solution is 3:1 to 5:

1.

4. The deodorant with compound long-lasting effect according to claim 1, characterized in that, The plant extract compound liquid contains, by volume, 3% to 8% of the plant extract, 1% to 5% of the rosemary essential oil, 1% to 5% of the cypress essential oil, 1% to 5% of the ethanol, 2% to 4% of the surfactant, and 75% to 92% of the water.

5. The deodorant with compound long-lasting effect according to claim 4, characterized in that, The plant extract compound liquid contains, by volume, 3% rosemary essential oil, 3% cypress essential oil, 4% monk fruit leaf extract, 4% black tiger leaf extract, 2% ethanol, 3% surfactant, and 81% water.

6. The deodorant with compound long-lasting effect according to claim 4, characterized in that, The plant extract compound liquid contains, by volume, 3% rosemary essential oil, 3% cypress essential oil, 3% monk fruit leaf extract, 3% black tiger leaf extract, 2% ethanol, 3% surfactant, and 83% water.

7. The deodorant with compound long-lasting effect according to claim 4, characterized in that, The viable bacteria content is 1.5-2.5 OD. 560 .

8. A method for deodorizing using a compound long-acting deodorant according to any one of claims 1 to 7, characterized in that, The method involves first spraying a compound solution of plant extracts, including monk fruit leaf extract, black tiger leaf extract, rosemary essential oil, and cypress essential oil, onto the target area for rapid deodorization. The rosemary essential oil and cypress essential oil have a synergistic effect to enhance the deodorization effect. The rosemary essential oil and cypress essential oil are obtained by steam distillation. The pH value of the microbial compound solution is adjusted to slightly acidic. The microbial compound solution is used in conjunction with the plant extracts to enhance the long-lasting effect.

9. The application of the compound long-acting deodorant according to any one of claims 1 to 7, characterized in that, The deodorizing agent is used to deodorize one of the following: landfills, garbage transfer stations, public toilets, urban septic tanks, black and odorous water bodies, organic fertilizer plants, and livestock farms.

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