Artificial breeding method of horrid bug and application thereof in prevention and control of mulberry pyralid

Abstract

The application discloses a breeding method of Harpactor fuscipes and application thereof in prevention and control of mulberry pyralid, and belongs to the technical field of pest control. The application comprises the following steps: (1) capturing adult Harpactor fuscipes from the wild, feeding, oviposition, and then hatching and breeding the eggs; (2) 1st instar nymphs: from hatching to the first molting, a mixed solution is used to dip a cotton ball to obtain a mixed solution cotton ball, the mixed solution cotton ball is placed in a hatching box, and the hatched Harpactor fuscipes feeds by itself; (3) after 2nd instar: in addition to the mixed solution cotton ball in the step (2), the 2nd instar to 4th instar Harpactor fuscipes is fed with artificial feed of corresponding instar silkworm, and the 5th instar Harpactor fuscipes and adult Harpactor fuscipes is fed with 4th instar artificial feed of silkworm. The method has high breeding rate and low cost, and the bred Harpactor fuscipes has remarkable effect in application in prevention and control of mulberry pyralid.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of pest control, in particular to a breeding method of Orius sauteri and its application in the control of the mulberry pyralid. BACKGROUND

[0002] Orius sauteri belongs to the family of Reduviidae and is an important predatory natural enemy. The predatory range of the bug is wide, and it particularly likes to eat Lepidoptera larvae. At present, the experimental research on the agricultural pests such as Spodoptera frugiperda, armyworm, Spodoptera litura, and corn borer and the green control of the mulberry pyralid in the production of Yunnan flue-cured tobacco are mainly carried out at home and abroad. Some biological characteristics and artificial feeding of Orius sauteri have been studied by experts of South China Agricultural University and Guizhou University and other colleges and universities. However, the above researches are all laboratory theoretical researches, and there is still a lack of efficient artificial breeding technology, which cannot achieve the application in actual agricultural production. The exploration of the efficient breeding, predatory function and field application mode of Orius sauteri shows that the application research of biological control of pests is still in the blank.

[0003] The mulberry pyralid is an insect of the genus of the wild silkworm moth and mainly feeds on mulberry trees. The mulberry pyralid is one of the most serious pest species in mulberry gardens, and it is easy to cause disaster due to its huge quantity, multiple generations, fast reproduction and strong resistance. Moreover, the mulberry pyralid carries pathogens such as viruses, microsporidia and bacteria, which can infect silkworms and induce silkworm diseases. The mulberry pyralid damages summer and autumn mulberry leaves with its larvae, and the damage to late autumn mulberry leaves is the heaviest. When the damage is serious, it causes the decrease of the yield and the deterioration of the quality of mulberry leaves, which seriously affects the production of sericulture.

[0004] The research on the efficient breeding technology of Orius sauteri and the field control technology of the mulberry pyralid has the significance of achieving the purpose of practical popularization and application, continuously reducing the base number of the mulberry pyralid in the garden, reducing the amount of pesticide use and realizing the reduction of the use of chemical pesticides.

[0005] The current artificial breeding method of Orius sauteri mostly uses yellow mealworms as the main food source, and some use the larvae of Spodoptera frugiperda as the food source of Orius sauteri. The breeding efficiency of the bug is low when yellow mealworms are used as the food source, and the average breeding rate of Orius sauteri to the 5th instar nymph or adult does not reach 50%. The cumulative egg production of a single female bug is less than 100. The cost of using Spodoptera frugiperda as the food source of the bug is extremely high, and the technical breeding of Spodoptera frugiperda is difficult, so it is difficult to achieve the effect of industrialized production and application. SUMMARY

[0006] The present application aims to solve the above problems in the prior art and provides a breeding method of Orius sauteri and its application in the control of the mulberry pyralid. The present application uses artificial feed to breed silkworms and uses the corresponding formula as the feeding food source and the innovative feeding method, which has a high breeding rate, reduces the cost, uses the silkworm bred by artificial feed as the food source, solves the problem of factory production in four seasons, achieves the efficient artificial breeding of Orius sauteri and meets the needs of large-scale industrialized production.

[0007] To achieve the above objectives, the technical solution of the present invention is as follows:

[0008] An artificial breeding method for the forked-horned bug includes the following steps:

[0009] (1) Capture adult Forked Horned Bugs from the wild, raise them, let them lay eggs, and then hatch and reproduce the eggs;

[0010] (2) First instar nymphs: From hatching to the first molt, use the mixture to soak dehydrated cotton balls to obtain the mixture cotton balls. Place the mixture cotton balls in the hatching box and let the forked bugs feed on themselves after hatching.

[0011] The mixture is prepared by uniformly mixing honey water, silkworm fluid, L1, and L2 in a mass ratio of 1-2:1-2:2-4:1-2.

[0012] The honey water is prepared by mixing honey and water at a mass ratio of 1:40-60.

[0013] The silkworm fluid is prepared by mixing silkworm body fluid and water at a mass ratio of 1:2-5.

[0014] L1 is prepared by mixing Lactobacillus acidophilus with 40-60 times the amount of water and honey with 40-60 times the amount of water until homogeneous.

[0015] The L2 is prepared by dissolving 0.3-0.8g of propolis, 600-700 units of vitamin A, 10-20mg of vitamin C, 300-500 units of vitamin D, 80-120mg of vitamin B, and 10-15g of excipients in 350ml of water. The excipients are made of sugar, sugarcane juice, and gelatin in a mass ratio of 1-2:2-4:0.5-1. The sugar can be white sugar or brown sugar.

[0016] (3) After the 2nd instar: In addition to feeding the mixed liquid cotton balls mentioned in step (2), the 2nd to 4th instar forked bugs are fed with the corresponding artificial feed for the silkworms, and the 5th instar forked bugs and adult forked bugs are fed with the 4th instar artificial feed for the silkworms.

[0017] Furthermore, in step (1), the rearing and hatching environment of the adult Forked Horned Bug is controlled as follows: temperature 26-28℃, humidity 80%, light 16L:8D.

[0018] Furthermore, in step (2), the silkworm body fluid is obtained by crushing live silkworms and centrifuging them.

[0019] Furthermore, the live silkworms are selected from those raised on artificial feed, and the specific rearing method is as follows: 1st and 2nd instar silkworms are fed artificial compound feed; 3rd instar silkworms are fed artificial compound feed and mulberry leaf powder, with artificial compound feed accounting for 90-95% and mulberry leaf powder accounting for 5-10%; 4th instar silkworms are fed artificial compound feed and mulberry leaf powder, with artificial compound feed accounting for 80-85% and mulberry leaf powder accounting for 15-20%.

[0020] Furthermore, in step (2), the Lactobacillus acidophilus count is 200 million / ml.

[0021] Furthermore, during the rearing process, the forked-horned bugs were raised separately at different ages, with potted hanging baskets placed in the rearing cages to artificially create a habitat for natural enemies, with a temperature of 27±1℃, humidity of 80%, and light intensity of 16L:8D.

[0022] The present invention also provides the application of the forked-horned bug bred by the artificial breeding method in the control of mulberry moth. When the number of adult female moths of mulberry moth is expected to reach 10-20 per day, 90-120 3rd-4th instar nymphs of the forked-horned bug bred by the artificial breeding method are released per mulberry orchard within 10-15 days after the moths emerge.

[0023] The beneficial effects of this invention are as follows:

[0024] 1. The artificial breeding method of the present invention results in high egg production and survival rate of the forked-horned bug. Experiments show that the adult rate of the forked-horned bug bred using the present invention reaches 74.55%, which is 21.10% higher than the control (fed with mealworms) adult rate of 53.45%; the egg production is also high, with an average cumulative egg production of 224 eggs per female adult, an increase of 156 eggs compared to the control (68 eggs) fed with mealworms, representing a 3.29-fold increase.

[0025] 2. The artificial breeding method of this invention is low-cost, highly efficient, and can be industrialized. Calculating the cost of breeding 100,000 *Pterocarya stenoptera*, the input is 0.55 yuan per insect. The cost of using fall armyworms for breeding is 3.50 yuan per insect, meaning this invention saves 6.36 times the cost. The cost of using mealworms for breeding is 2.13 yuan per insect, meaning this invention saves 3.87 times the cost.

[0026] 3. The artificial breeding method of this invention produces larger, more active, and more predatory *Pterocarya stenoptera* individuals. A comparison of the effects of *Pterocarya stenoptera* raised using different methods on controlling the mulberry leafminer showed that the *Pterocarya stenoptera* bred using the method of this invention achieved a mulberry leafminer population reduction rate of 73.81% and 97.61% after 7 and 17 days of application, respectively, which were 13-18 percentage points higher than those raised using fall armyworm and mealworm.

[0027] 4. The forked-horned bug is a widely used natural enemy insect. It can be used not only for the control of major mulberry tree pests such as the mulberry borer, but this invention has also shown that the forked-horned bug can control lepidopteran pests on other crops, such as the fall armyworm, beet armyworm, armyworm, corn borer, rice stem borer, cabbage caterpillar, cutworm larvae, rice weevils, and many other pests. Attached Figure Description

[0028] Figure 1 Summary table of comparative experiments on the culture of the forked-horned bug;

[0029] Figure 2 This is a statistical table of the application experiment of the forked-horned bug in the control of the mulberry leafminer. Detailed Implementation

[0030] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to the embodiments and accompanying drawings.

[0031] Example 1

[0032] An artificial breeding method for the forked-horned bug includes the following steps:

[0033] (1) Adult dominant forked bugs were captured from the local wild and raised in an environment with a temperature of 26-28℃, humidity of 80%, and light intensity of 16L:8D (16h day and 8h night). They were fed with artificial feed for 4th instar silkworms to lay eggs, and then the eggs were placed in a rearing room under the above environmental conditions to promote hatching and reproduction.

[0034] (2) First instar nymphs: From hatching to the first molt, use the mixture to soak dehydrated cotton balls to obtain the mixture cotton balls. Place the mixture cotton balls in the hatching box and let the forked bugs feed on themselves after hatching.

[0035] The mixture is prepared by mixing honey water, silkworm fluid, L1, and L2 in a mass ratio of 1:1:2:1.

[0036] The honey water is prepared by mixing honey and water at a mass ratio of 1:50.

[0037] The silkworm fluid is prepared by mixing silkworm body fluid and water at a mass ratio of 1:3. The silkworm body fluid is prepared by crushing live silkworms and centrifuging them. The live silkworms are selected from domesticated silkworms that have been raised on artificial feed in their fourth instar.

[0038] The L1 was prepared by mixing Lactobacillus acidophilus (200 million cells / ml) with water at a ratio of 50 by weight and honey at a ratio of 50 by weight.

[0039] The L2 is made by mixing 0.5g of propolis, 620 units of vitamin A, 15mg of vitamin C, 400 units of vitamin D, 100mg of vitamin B, and 12g of adjuvants in 350ml of water. The adjuvants are made of brown sugar, sugarcane juice, and gelatin in a mass ratio of 2:4:1.

[0040] (3) After the 2nd instar: In addition to feeding the mixed liquid cotton balls described in step (2), the 2nd to 4th instar forked bugs are fed with the corresponding artificial feed for the silkworms raised. The 5th instar forked bugs and adult forked bugs are fed with the 4th instar artificial feed for the silkworms raised. The adult forked bugs obtained in step (3) can be used as adult forked bugs from step (1) for oviposition and subsequent artificial breeding.

[0041] The silkworms raised on artificial feed in steps (1)-(3) are raised in the following specific ways: 1st and 2nd instar silkworms are fed artificial compound feed; 3rd instar silkworms are fed artificial compound feed and mulberry leaf powder, with artificial compound feed accounting for 90% and mulberry leaf powder accounting for 10%; 4th instar silkworms are fed artificial compound feed and mulberry leaf powder, with artificial compound feed accounting for 80% and mulberry leaf powder accounting for 20%. The artificial compound feed is selected from the silkworm compound feed of Chongqing Zhengjia Feed Co., Ltd.

[0042] During the rearing process, the aforementioned forked-horned bugs need to be raised separately for each age group, and different ages should not be mixed in the same rearing cage to avoid cannibalism. Potted hanging baskets should be placed in the rearing cages to artificially create a living environment for natural enemies, with a temperature of 27±1℃, humidity of 80%, and light intensity of 16L:8D (16 hours of daylight and 8 hours of nightlight).

[0043] Example 2

[0044] An artificial breeding method for the forked-horned bug includes the following steps:

[0045] (1) Adult dominant forked bugs were captured from the local wild and raised in an environment with a temperature of 26-28℃, humidity of 80%, and light intensity of 16L:8D (16h day and 8h night). They were fed with artificial feed for 4th instar silkworms to lay eggs, and then the eggs were placed in a rearing room under the above environmental conditions to promote hatching and reproduction.

[0046] (2) First instar nymphs: From hatching to the first molt, use the mixture to soak dehydrated cotton balls to obtain the mixture cotton balls. Place the mixture cotton balls in the hatching box and let the forked bugs feed on themselves after hatching.

[0047] The mixture is prepared by mixing honey water, silkworm fluid, L1, and L2 in a mass ratio of 1:2:2:1.

[0048] The honey water is prepared by mixing honey and water at a mass ratio of 1:40.

[0049] The silkworm fluid is prepared by mixing silkworm body fluid and water at a mass ratio of 1:2. The silkworm body fluid is prepared by crushing live silkworms and centrifuging them. The live silkworms are selected from 4th instar domestic silkworms fed with artificial feed.

[0050] The L1 was prepared by mixing Lactobacillus acidophilus (200 million / ml) with water at a 40-fold dilution and honey with water at a 40-fold dilution.

[0051] The L2 is made by dissolving 0.8g of propolis, 700 units of vitamin A, 20mg of vitamin C, 500 units of vitamin D, 20mg of vitamin B1, and 15g of adjuvants in 350ml of water. The adjuvants are made of white sugar, sugarcane juice, and gelatin in a mass ratio of 2:3:0.8.

[0052] (3) After the 2nd instar: In addition to feeding the mixed liquid cotton balls mentioned in step (2), the 2nd to 4th instar forked bugs are fed with the corresponding artificial feed for the silkworms, and the 5th instar forked bugs and adult forked bugs are fed with the 4th instar artificial feed for the silkworms.

[0053] The specific feeding methods for silkworms raised with artificial feed in steps (1)-(3) are as follows: 1st and 2nd instar silkworms are fed artificial compound feed; 3rd instar silkworms are fed artificial compound feed and mulberry leaf powder, of which artificial compound feed accounts for 95% and mulberry leaf powder accounts for 5%; 4th instar silkworms are fed artificial compound feed and mulberry leaf powder, of which artificial compound feed accounts for 85% and mulberry leaf powder accounts for 15%.

[0054] During the rearing process, the aforementioned forked-horned bugs need to be raised separately for each age group, and different ages should not be mixed in the same rearing cage to avoid cannibalism. Potted hanging baskets should be placed in the rearing cages to artificially create a living environment for natural enemies, with a temperature of 27±1℃, humidity of 80%, and light intensity of 16L:8D (16 hours of daylight and 8 hours of nightlight).

[0055] Example 3

[0056] An artificial breeding method for the forked-horned bug includes the following steps:

[0057] (1) Adult dominant forked bugs were captured from the local wild and raised in an environment with a temperature of 26-28℃, humidity of 80%, and light intensity of 16L:8D (16h day and 8h night). They were fed with artificial feed for 4th instar silkworms to lay eggs, and then the eggs were placed in a rearing room under the above environmental conditions to promote hatching and reproduction.

[0058] (2) First instar nymphs: From hatching to the first molt, use the mixture to soak dehydrated cotton balls to obtain the mixture cotton balls. Place the mixture cotton balls in the hatching box and let the forked bugs feed on themselves after hatching.

[0059] The mixture is prepared by mixing honey water, silkworm fluid, L1, and L2 in a mass ratio of 1:1:2:1.

[0060] The honey water is prepared by mixing honey and water at a mass ratio of 1:60.

[0061] The silkworm fluid is prepared by mixing silkworm body fluid and water at a mass ratio of 1:5. The silkworm body fluid is prepared by crushing live silkworms and then centrifuging them. The live silkworms are selected from domesticated silkworms that have been raised on artificial feed in their fourth instar.

[0062] The L1 was prepared by mixing Lactobacillus acidophilus (200 million / ml) with water at a ratio of 60 and honey at a ratio of 60;

[0063] The L2 is made by dissolving 0.3g of propolis, 600 units of vitamin A, 10mg of vitamin C, 300 units of vitamin D, 80mg of vitamin B, and 10g of adjuvants in 350ml of water. The adjuvants are made of brown sugar, sugarcane juice, and gelatin in a mass ratio of 1.5:2:0.5.

[0064] (3) After the 2nd instar: In addition to feeding the mixed liquid cotton balls mentioned in step (2), the 2nd to 4th instar forked bugs are fed with the corresponding artificial feed for the silkworms, and the 5th instar forked bugs and adult forked bugs are fed with the 4th instar artificial feed for the silkworms.

[0065] The specific feeding methods for silkworms raised with artificial feed in steps (1)-(3) are as follows: 1st and 2nd instar silkworms are fed artificial compound feed; 3rd instar silkworms are fed artificial compound feed and mulberry leaf powder, with artificial compound feed accounting for 90% and mulberry leaf powder accounting for 10%; 4th instar silkworms are fed artificial compound feed and mulberry leaf powder, with artificial compound feed accounting for 80% and mulberry leaf powder accounting for 20%.

[0066] During the rearing process, the aforementioned forked-horned bugs need to be raised separately for each age group, and different ages should not be mixed in the same rearing cage to avoid cannibalism. Potted hanging baskets should be placed in the rearing cages to artificially create a living environment for natural enemies, with a temperature of 27±1℃, humidity of 80%, and light intensity of 16L:8D (16 hours of daylight and 8 hours of nightlight).

[0067] Example 4

[0068] An artificial breeding method for the forked-horned bug includes the following steps:

[0069] (1) Adult dominant forked bugs were captured from the local wild and raised in an environment with a temperature of 26-28℃, humidity of 80%, and light intensity of 16L:8D (16h day and 8h night). They were fed with artificial feed for 4th instar silkworms to lay eggs, and then the eggs were placed in a rearing room under the above environmental conditions to promote hatching and reproduction.

[0070] (2) First instar nymphs: From hatching to the first molt, use the mixture to soak dehydrated cotton balls to obtain the mixture cotton balls. Place the mixture cotton balls in the hatching box and let the forked bugs feed on themselves after hatching.

[0071] The mixture is prepared by mixing honey water, silkworm fluid, L1, and L2 in a mass ratio of 1:1:2:2.

[0072] The honey water is prepared by mixing honey and water at a mass ratio of 1:50.

[0073] The silkworm fluid is prepared by mixing silkworm body fluid and water at a mass ratio of 1:4. The silkworm body fluid is prepared by crushing live silkworms and centrifuging them. The live silkworms are selected from domesticated silkworms that have been raised on artificial feed in their fourth instar.

[0074] The L1 was prepared by mixing Lactobacillus acidophilus (200 million cells / ml) with water at a 50-fold dilution and honey at a 50-fold dilution.

[0075] The L2 is made by dissolving 0.4g of propolis, 650 units of vitamin A, 12mg of vitamin C, 400 units of vitamin D, 90mg of vitamin B, and 10g of adjuvants in 350ml of water. The adjuvants are made of white sugar, sugarcane juice, and gelatin in a mass ratio of 1:3:0.6.

[0076] (3) After the 2nd instar: In addition to feeding the mixed liquid cotton balls mentioned in step (2), the 2nd to 4th instar forked bugs are fed with the corresponding artificial feed for the silkworms, and the 5th instar forked bugs and adult forked bugs are fed with the 4th instar artificial feed for the silkworms.

[0077] The specific feeding methods for silkworms raised with artificial feed in steps (1)-(3) are as follows: 1st and 2nd instar silkworms are fed artificial compound feed; 3rd instar silkworms are fed artificial compound feed and mulberry leaf powder, with artificial compound feed accounting for 90% and mulberry leaf powder accounting for 10%; 4th instar silkworms are fed artificial compound feed and mulberry leaf powder, with artificial compound feed accounting for 80% and mulberry leaf powder accounting for 20%.

[0078] During the rearing process, the aforementioned forked-horned bugs need to be raised separately for each age group, and different ages should not be mixed in the same rearing cage to avoid cannibalism. Potted hanging baskets should be placed in the rearing cages to artificially create a living environment for natural enemies, with a temperature of 27±1℃, humidity of 80%, and light intensity of 16L:8D (16 hours of daylight and 8 hours of nightlight).

[0079] Comparative Example 1

[0080] A method for artificially breeding *Triplophysa fasciata* is based on Example 1, wherein the mixture is prepared by uniformly mixing honey water and silkworm sap in a mass ratio of 1:1, and the rest is the same as in Example 1.

[0081] Comparative Example 2

[0082] A method for artificially breeding *Triplophysa fasciata*, based on Example 1, wherein the mixture is prepared by uniformly mixing honey water, silkworm sap, and L1 in a mass ratio of 1:1:2, and the rest is the same as in Example 1.

[0083] Comparative Example 3

[0084] An artificial breeding method for the forked-horn bug is based on Example 1, wherein the mixture is prepared by uniformly mixing honey water, silkworm sap, and L2 in a mass ratio of 1:1:1, and the rest is the same as in Example 1.

[0085] Comparative Example 4

[0086] An artificial breeding method for *Tridentus cristatus* is based on Example 1, except that the mixture is replaced with water, while everything else remains the same as in Example 1.

[0087] Comparative Example 5

[0088] An artificial breeding method for the forked-horn bug is based on Example 1, except that the mixture is replaced with clean water. After the second instar, in addition to feeding the bug with clean water cotton balls, the bug is fed with mealworm pupae. Otherwise, the method is the same as in Example 1.

[0089] like Figure 1 The table shown is a summary of the comparative experiments on the breeding of *Triplophysa fasciata* in 2023. The adult survival rate in the table is the percentage of adults that did not die by the time egg-laying began. The adult rate is the number of adults divided by the number of adults at hatching. The number of adults refers to the number of fifth-instar nymphs that molt into adults. The data in the table shows that the method in Example 1 of this invention resulted in high reproductive and adult rates for *Triplophysa fasciata*. Comparative Examples 1-4 showed a decrease in both adult and reproductive rates due to changes in the mixed solution formula. Comparative Example 5, which used mealworms, had the lowest reproductive and adult rates. The adult rate of *Triplophysa fasciata* bred using this invention reached 74.55%, which is 21.10% higher than the 53.45% adult rate achieved with mealworms. Egg production was also high, with an average of 224 eggs laid per female adult, an increase of 156 eggs (3.29 times) compared to the 68 eggs laid by mealworms.

[0090] Application Examples

[0091] When the number of female mulberry borers is expected to reach 10-20 per day, 100 3rd-4th instar nymphs of the forked-horned bug are released per mulberry orchard within 10-15 days after the borers emerge.

[0092] like Figure 2The table shown is a statistical table of the application experiment of *Triplophysa gracilis* in the control of mulberry leafminer using the above method. Experimental area 1 in the table shows *Triplophysa gracilis* bred using the method of Example 1 of this invention; experimental area 2 shows *Triplophysa gracilis* reared using *Pseudomonas aeruginosa*; experimental area 3 shows *Triplophysa gracilis* reared using mealworms; and the control group uses the local conventional control method (i.e., applying the pesticide dichlorvos only once a year during silkworm rearing in spring or autumn). Figure 2 Data comparison shows that the population decline rate of the mulberry borer after 7 days and 17 days of application of the *Triplophysa gracilis* bred by the method of this invention was 73.81% and 97.61%, respectively, which was 13-18 percentage points higher than that of the *Triplophysa gracilis* raised using fall armyworm and mealworm.

[0093] Furthermore, calculating the cost of breeding 100,000 *Triplophysa gracilis*, the input is 0.55 yuan per insect, while the cost of using fall armyworm for breeding is 3.50 yuan per insect. This invention saves 6.36 times the cost. Using mealworms for breeding costs 2.13 yuan per insect, resulting in a cost saving of 3.87 times the cost of this invention. In summary, the method of this invention not only improves the breeding rate of *Triplophysa gracilis* but also significantly reduces breeding costs, and its application in controlling mulberry leafminer is also more effective.

[0094] Although the present invention has been disclosed above with reference to preferred embodiments, it is not intended to limit the present invention. Any person skilled in the art can make some modifications and improvements without departing from the spirit and scope of the present invention. Therefore, the scope of protection of the present invention shall be defined by the claims.

Claims

1. A method for the artificial breeding of the forked-horned bug, characterized in that, Includes the following steps: (1) Capture adult Forked Horned Bugs from the wild, raise them, let them lay eggs, and then hatch and reproduce the eggs; (2) First instar nymphs: From hatching to the first molt, use the mixture to soak dehydrated cotton balls to obtain the mixture cotton balls. Place the mixture cotton balls in the hatching box and let the forked bugs feed on themselves after hatching. The mixture is prepared by uniformly mixing honey water, silkworm fluid, L1, and L2 in a mass ratio of 1-2:1-2:2-4:1-2. The honey water is prepared by mixing honey and water at a mass ratio of 1:40-60. The silkworm fluid is prepared by mixing silkworm body fluid and water at a mass ratio of 1:2-5; the silkworm body fluid is prepared by crushing live silkworms and then centrifuging them. L1 is prepared by mixing Lactobacillus acidophilus with 40-60 times the amount of water and honey with 40-60 times the amount of water until homogeneous. The L2 is made by dissolving 0.3-0.8g of propolis, 600-700 units of vitamin A, 10-20mg of vitamin C, 300-500 units of vitamin D, 80-120mg of vitamin B, and 10-15g of adjuvants in 350ml of water. The adjuvants are made of sugar, sugarcane juice, and gelatin in a mass ratio of 1-2:2-4:0.5-1. (3) After the 2nd instar: In addition to feeding the mixed liquid cotton balls mentioned in step (2), the 2nd to 4th instar forked bugs are fed with the corresponding artificial feed for the silkworms, and the 5th instar forked bugs and adult forked bugs are fed with the 4th instar artificial feed for the silkworms.

2. The method for artificially breeding *Trigonella foetida* according to claim 1, characterized in that: In step (1), the rearing and hatching environment of the adult Forked Horned Bug is controlled as follows: temperature 26-28℃, humidity 80%, light 16L:8D.

3. The method for artificially breeding *Trigonella foetida* according to claim 1, characterized in that: The live silkworms are domesticated silkworms raised on artificial feed. The specific rearing methods are as follows: 1st and 2nd instar silkworms are fed artificial compound feed; 3rd instar silkworms are fed artificial compound feed and mulberry leaf powder, with artificial compound feed accounting for 90-95% and mulberry leaf powder accounting for 5-10%; 4th instar silkworms are fed artificial compound feed and mulberry leaf powder, with artificial compound feed accounting for 80-85% and mulberry leaf powder accounting for 15-20%.

4. The method for artificially breeding *Trigonella foetida* according to claim 1, characterized in that: In step (2), the Lactobacillus acidophilus count is 200 million / ml.

5. The method for artificially breeding *Trigonella foetida* according to claim 1, characterized in that: During the rearing process, each age group of the forked-horned bug was raised separately. Potted spider plants were placed in the rearing cages to artificially create a living environment for natural enemies. The temperature was 27±1℃, the humidity was 80%, and the light intensity was 16L:8D.

6. The application of the forked-horned bug in the control of the mulberry leafminer, characterized by: When the number of adult female mulberry moths is expected to reach 10-20 per day, 90-120 3rd-4th instar nymphs of the artificially bred *Pterocarya stenoptera* (as described in claim 1) should be released per mulberry orchard within 10-15 days after the moths emerge.

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